JPH05503760A - Coupling in separation systems - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Coupling in separation systems The present invention is a separation unit removably mounted on a stand, each Has an intake opening and an extraction opening that are connected to the inlet and outlet via a coupling. Coupling in separation systems with separation units, especially gel filters Regarding. Each of the couplers is separated with a portion of the coupler mounted on the stand. and a coupler portion attached to the unit.

Separation systems for separating and purifying biomolecules, e.g. gel chromatography In this process, column packing is a very important operation. The column is poorly packed. Otherwise, the flow direction and flow rate will become non-uniform, and the separation performance will decrease. When filling the column , it is essential to create a homogeneous bed with a uniform surface. bed surface If it is non-uniform, the separation band will be non-uniform and the separation performance will be reduced. Therefore, column For example, filling gel filter media is often a time-consuming operation. It is a business.

The object of the invention is to connect the separation unit in such a way that the pressure in the separation unit can be maintained. The present invention provides a coupling for a separation system that enables connection and separation.

To achieve the above object, the coupling of the invention has the features as claimed in claim 1. have.

The present invention will now be described with reference to the accompanying drawings.

FIG. 1 is a side view of a separation system in which a filter - An exchangeable unit with a medium, e.g. a gel column, is connected to the upper coupling and the lower coupling. is connected to the separation system by a coupling. Both upper and lower cups The two systems are constructed according to the invention and are identical.

Figure 2 shows the closed position, i.e. when the separation unit is removed from the separation system. FIG. 3 is a longitudinal cross-sectional view showing the coupling in position;

Figure 3 shows the open position, i.e. the position when the separation unit is connected to the separation system. 3 is a longitudinal cross-sectional view corresponding to FIG. 2 showing the coupling in position; FIG.

FIG. 4 is a cross-sectional view taken along line IV-IV in FIG.

FIG. 5 is a sectional view taken along the line ■-■ in FIG.

FIG. 6 is a sectional view taken along line VI-VI in FIG.

FIG. 7 is a sectional view taken along the line ■--■ in FIG.

In FIG. 1, the separation unit 11 includes an upper coupling 12A and a lower coupling. It is removably attached to the stand IO by a tag 12B. Upper and lower parts Pulling +2A.

12B are the same, and each part of the coupler attached to the stand 10 + 5A, 15B and part of the coupler attached to the separation unit 11 + 6A, 16B It has Details of coupler portions 15A, 15B are shown in FIGS. 2-7. There is. Each coupler part has a connection socket I3 and also has a connection socket I3. Nokesoto 13 is provided with a passage 13A, and this passage +3A is connected to the upper coupler. Part 15A constitutes the intake port, and part 5B of the lower coupler constitutes the intake port. are doing. Each of the coupler portions further comprises a closing member 17, which closes the The chain member 17 is displaced over the coupler portion 15A (see FIG. 7) by the U-shaped portion. and the closing member includes a rod displaceably guided in the coupler portion. It is connected to one end of the head 17A. The closing member 17 is connected to the other end of the rod 17A and the upper end of the rod 17A. A tension spring 19 (see FIG. 2) installed between the coupler and a portion of the coupler held in place. In this closed position, the closing member closes the passageway 13A with a gasket. Keep it closed at 13B. Correspondingly, coupler part +6A, 1 6B each have an inlet and an outlet connection socket 14, and these connection sockets The ket is connected to the separation unit 11 and has a passageway 14A. coupler one The parts 16A, 16B furthermore have a displaceably guided closure member 18, which The chain member 18 is held in the closed position by a compression spring 20, and the passage +4A is closed by a gasket. It is closed at gate 14B (see FIG. 2).

Coupler parts 15A and 158゛ attached to stand IO and separation unit Coupler portion 16A attached to 11.

When each of 16B is separated, the passageway of the image portion is closed.

When the separation unit 11 is inserted into the separation system, the stop member 1 of both coupler parts 7.18 is pressed and as a result resists the spring force due to the parts of both couplers pushing against each other. Then, it becomes the open position. At that time, it engages with the closing member 17 or the gasket 14B, Moves away from gasket I3. At the same time, coupler part I5Δ, 15B are engaged with the closing member I8, respectively, and move the member away from the gas get 14B. move to As a result, the two couplings can be connected by a coupler section that can be interlocked. 3A and 14A are aligned and the gaskets 13B and 14B engage with each other. The passages 13A and 14A communicate with each other.

~ FIG, 4 FIG, 5 abstract A separation system having a separation unit (11) removably mounted on a stand (10) a coupling in the stem, the separation units each having a coupling in the stem; The inlet connection socket and the connection socket connected to the connection socket via the Equipped with an extraction connection socket. Each coupling is mounted on a stand. Part of the coupler (15A, 15B) and part of the coupler attached to the separation unit (16A, 16B), and furthermore, each coupler portion has a biasing action of a spring. and a spring-biased closure member held in the closed position by. in one coupler Each closing member of the two included coupler parts is connected to a separating unit ( 11) into the stand (10), some of the couplers may engage against each other. By matching, it can be displaced into the open position against the biasing action of the spring.

Procedural amendment August 8, 1992

Claims (1)

[Claims] 1. A component consisting of multiple couplings (12A, 12B) and having a separation unit. couplings in a separate system, each coupling having a stand (10) Attached to the coupler part (15A, 15B) and separation unit (11) attached to the the separation unit (11) has coupler portions (16A, 16B) separated from each other; It is removably attached to the stand (10), and the separation unit (11 ) are respectively connected to the connection unit (13) via couplings (12A, 12B). Those having an intake connection socket and an output connection socket (14) to be connected. , each coupler part (15A, 15B, 16A, 16B) is operated by spring deflection. a closing member (17, 18) which is held in a closed position depending on the use; When the knit (11) is attached to the stand (10), the coupler parts should not touch each other. The two coupler parts of each coupling are connected by resisting engagement. The closure member is displaceable into the open position against the biasing action of a spring. Coupling in remote systems.