JPH053788A - Apparatus for electrical treatment of cell - Google Patents

Abstract

PURPOSE:To enable easy recognition of progress conditions of electrical treatment during the electrical treatment of cells. CONSTITUTION:An apparatus 1 for electrical treatment of cells is equipped with a chamber 2 having opposite electrodes, a controlling part 20 for controlling fusing operation of plural kinds of cells and a display panel 8 for displaying so as to enable recognition of operation during practice in fusing operation for the plural kinds of cells.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention
The present invention relates to a cell electroprocessing device for performing cell processing by applying a voltage to cells.

[0002]

2. Description of the Related Art As a conventional cell electroprocessing apparatus, a chamber having a counter electrode, a voltage applying unit connected to the counter electrode, and a control section for controlling a voltage applying operation to the voltage applying unit are provided. It has been known.

In this cell electrical processing apparatus, cells are arranged between opposing electrodes of the processing apparatus main body. Then, based on the control by the control unit, the voltage application unit performs a predetermined voltage application operation on the cell via the counter electrode. This allows
A pair of cells are in a fused state, or a new DN in the cell
A is introduced.

[0004]

The conventional cell electrical processing apparatus has a function of repeatedly applying an alternating voltage and a direct voltage to cells to be processed. However, the device does not inform the operator which process is being executed during the cell fusion process. For this reason, it is not easy for the operator to recognize the progress of cell electrical processing.

An object of the present invention is to provide a cell electroprocessing apparatus which can easily recognize the progress of processing during cell electroprocessing.

[0006]

The cell electrical processing apparatus according to the present invention is an apparatus for processing cells by applying a voltage to the cells. This apparatus has a chamber having counter electrodes, voltage applying means for applying a voltage between the counter electrodes, and control for controlling the voltage applying means so as to perform a plurality of types of voltage applying operations corresponding to a plurality of types of cell processing operations. And a display unit that displays so as to identify the operation being executed among the plurality of types of cell processing operations.

[0007]

In the cell electrical treatment apparatus according to the present invention, the cells to be treated are placed between the counter electrodes of the chamber. Then, the control means controls the voltage application means to perform a plurality of types of voltage application operations corresponding to a plurality of types of cell processing operations. Thereby, a predetermined voltage is applied between the counter electrodes,
Cell electrolysis is performed.

In the cell processing operation, the display section displays so that the operation being executed can be identified. As a result, the operator can easily recognize the progress of the process during the cell electrical process.

[0009]

EXAMPLE FIG. 1 shows a cell fusion device 1 as an example of the present invention. In the figure, the cell fusion device 1 is
Chamber 2, controller 3, power unit 4
And a temperature controller 5.

The chamber 2 is a substantially disk-shaped member, and has a counter electrode 6 consisting of a pair of electrodes arranged concentrically inside. The chamber 2 is mounted on the temperature controller 5. The temperature controller 5 is for keeping the cell suspension at a constant temperature during the cell fusion process.

The controller 3 has a key panel 7 and a display panel 8 arranged on the front part thereof, and a printer 9 arranged on the upper part thereof. The key panel 7 is shown in FIG.
, An arrow key 10 for instructing a direction, a stop key 11, a start key 12, and an input key 13
And other keys.

On the display panel 8, as shown in FIG. 3, a diagram showing various steps executed at the time of cell fusion is drawn. Further, the display panel 8 is provided with a process display lamp 14 composed of an LED corresponding to various processes performed at the time of cell fusion. Further, a numerical value display unit 15 including an LCD for displaying numerical values that need to be set in each step is provided.

The power unit 4 is composed of a transformer or the like, and can supply a voltage to the chamber 2.

The controller 3 has a control section 20 as shown in FIG. The control unit 20 includes a CPU, a ROM,
It includes a microcomputer such as a RAM.
The power unit 4, the temperature controller 5, the key panel 7, the display panel 8, and the printer 9 are connected to the I / O port 21 of the control unit 20.

Next, the operation of the above embodiment will be described with reference to FIGS.
It will be described based on the control flowchart of FIG. When the program starts, in step S1 of FIG. 5, initial settings such as display operation confirmation of the display panel 8 and initial posture control of the printer 9 are performed. Next, in step S2, it is determined whether or not the input key 13 of the key panel 7 is pressed by the operator in order to instruct to perform numerical value setting or the like in the cell fusion operation. If the input key 13 is not pressed, the process proceeds to step S3. In step S3, in order to instruct the start of the cell fusion operation, it is determined whether or not the start key 12 of the key panel 7 is pressed by the operator. If the start key 12 is not pressed,
After performing other general processing in step S4, the process returns to step S2. Here, if the input key 13 is pressed by the operator to change various set values of the cell fusion operation, the program proceeds from step S2 to step S5 and executes the input subroutine of FIG.
In step S7 of FIG. 6, it is determined whether or not the arrow key 10 has been pressed. If the arrow key 10 has not been pressed, the process proceeds to step S8, and it is determined whether the input key 13 has been pressed. If the input key 13 has not been pressed, the process proceeds to step S9, and it is determined whether the stop key 11 has been pressed. If the stop key 11 is not pressed, the program returns to step S7.

Here, if the operator presses the arrow key 10 to select the numerical value of the cell fusion process to be changed, the program proceeds from step S7 to step S1.
Move to 0. In step S10, the element to be changed is changed according to the pointing direction of the arrow key 10. Then, the numerical value display section 15 of the display panel 8 corresponding to the changed element is made to blink. Here, if the downward arrow key 10 is pressed, the selected position of the numerical value display unit 15 is lowered, and if the upward arrow key 10 is pressed, the selected position is moved upward. If the left arrow key 10 is pressed, the selected position of the numerical value display unit 15 moves to the left, and if the right arrow key 10 is pressed, the selected position moves to the right. When the input type changing process ends, the process in step S10 ends.

If the operator presses the input key 13 with the numerical display section 15 desired to be changed selected, the program moves from step S8 to step S11. Step S1
At 1, it is determined whether the up or down arrow key 10 has been pressed. If the upward or downward arrow key 10 is pressed, the process proceeds to step S12. In step S12, the set numerical value is increased when the upward arrow key 10 is pressed, and the set numerical value is decreased when the downward arrow key 10 is pressed. When the desired numerical value is obtained, the operator presses the stop key 11
push. As a result, the determination in step S13 is Yes.
Then, the numerical value changing process ends. If you complete the numerical change for all the numbers in the cell fusion process you want to change,
The operator presses the stop key 11. As a result, the determination in step S9 becomes Yes, and the input subroutine ends.

Next, after performing a preparatory operation such as setting the cells to be treated in the chamber 2, when the operator presses the start key 12, the program for starting the cell fusion operation is shown in FIG. The process moves from step S3 to step S14, and the processing subroutine of FIG. 7 is executed.

In FIG. 7, in step S16, the leftmost process display lamp 14 of the display panel 8 is turned on. As a result, the operator can easily recognize that the stage for applying the cell migration high frequency voltage has been entered. Then, in step S17, the high frequency voltage for cell migration is applied at the set voltage value and frequency for the set time.

Next, in step S18, the number of repetitions n is set to 0. Then, in step S19, the number of times n is incremented.

Then, in step S20, the process display lamp 14 corresponding to the stage for applying the square wave pulse for fusion is turned on. Then, in step S21,
DC voltage is applied at a predetermined voltage value.

When the processing in step S21 is completed, the process proceeds to step S22. In step S22, the process display lamp 14 corresponding to the next high frequency voltage application stage is turned on. Then, in step S23, a corresponding high frequency applying operation is performed.

When the processing in step S23 is completed, the process proceeds to step S24. Here, based on the number of repetitions n, it is determined whether or not all the commanded cell fusion operations have been performed. When it is determined that all the cell fusion operations have not been completed, the processing of step S19 and thereafter is repeated. In that case, the DC voltage and the high frequency voltage are attenuated based on the preset attenuation rate.

If it is determined in step S24 that all cell fusion steps have been completed, the program returns to the main routine of FIG.

Here, as the cell fusion process of plural kinds of cells progresses, the process display lamp 1 is displayed on the display panel 8.
Since the display of 4 is changed, the operator can easily recognize the process being executed.

[0026]

In the cell electrical processing apparatus according to the present invention, the display section displays so that the operation being executed among the plurality of types of cell processing operations can be recognized. Therefore, according to the present invention, it is possible to easily recognize the progress state of the treatment during the cell electrical treatment. Further, since the DC voltage application timing is known and the danger of application at that time is displayed, the safety for the operator is increased.

[Brief description of drawings]

FIG. 1 is a perspective view of a cell fusion device as one embodiment of the present invention.

FIG. 2 is a front view of the key panel.

FIG. 3 is a front view of the display panel.

FIG. 4 is a schematic block diagram of a control unit thereof.

FIG. 5 is a control flowchart thereof.

FIG. 6 is a control flowchart thereof.

FIG. 7 is a control flowchart thereof.

[Explanation of symbols]

 1 Cell fusion device 2 Chamber 4 Power unit 8 Display panel 20 Control unit

Claims (1)

Claim: What is claimed is: 1. A cell electroprocessing apparatus for treating a cell by applying a voltage to the cell, which comprises a chamber having a counter electrode and a voltage for applying a voltage between the counter electrodes. An application unit, a control unit that controls the voltage application unit to perform a plurality of types of voltage application operations corresponding to a plurality of types of cell processing operations, and an operation that is being executed among a plurality of types of cell processing operations can be identified. A cell electrical processing device comprising: