JPH05331205A - Production of branched cyclodextrin - Google Patents

Abstract

PURPOSE:To simply and inexpensively produce the branched cyclodextrin in a high yield by treating roasted dextrin with cyclodextrin glucanotransferase. CONSTITUTION:Roasted dextrin is treated with cyclodextrin glucanotransferase to produce the branched cyclodextrin. Preferably, a 4-20% aqueous solution of the roasted dextrin is treated with 5-20 units of the cyclodextrin glucanotransferase at a pH of 5-9.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing a branched cyclodextrin.

[0002]

2. Description of the Related Art Cyclodextrin (hereinafter abbreviated as CD) contains 6 or more glucose and α-1,4 in a ring.
It is a linked oligosaccharide, and because of its special inclusion action,
It is widely used in foods and other fields for the purpose of stabilizing flavors and spices, masking specific odors, and the like. Depending on the number of glucose residues forming a ring, α- (6), β- (7
Individual) and γ- (8) CDs. These CDs have a problem that their solubility in water is low. To solve this, a method for producing maltosylated CDs by utilizing a reverse reaction of CDs with a debranching enzyme, or using branched dextrin as a raw material , Cyclodextrin glucanotransferase (hereinafter, abbreviated as CGT-ase) is allowed to act to produce branched CD, and further enzymatic treatment is performed to finally produce glucosylated CD. (For example, Kozo Hara, Monthly Food Chemical, 1989-5, p2.
7-36)

[0003]

Among the above methods, the production of maltosylated CD by debranching enzyme has a problem that a large amount of debranching enzyme is required. The latter method is economically disadvantageous because it uses expensive branched dextrin obtained by enzymatically decomposing starch and fractionating and purifying. At present, there is no branched dextrin that can be produced simply and inexpensively.

[0004]

Means for Solving the Problems In order to solve the above-mentioned problems, the inventors of the present invention have conducted extensive studies and as a result, when roasted dextrin was used as a raw material and CGT-ase was allowed to act on it, branched dextrin was obtained in high yield. The inventors have found that they are generated and have completed the present invention.

[0005] Roasted dextrin is obtained by heating and roasting starch with a small amount of mineral acid using a roaster or the like, and only by acid hydrolysis by a wet process or simple hydrolysis reaction such as enzyme-modified dextrin. Instead, it has a highly branched structure due to decomposition and repolymerization reactions.

Examples of the raw material starch of the roasted dextrin of the present invention include wheat starch, potato starch, corn starch, sweet potato starch, tapioca starch, sago starch, rice starch, waxy corn starch, high amylose corn starch and the like. Examples thereof include treated starch, and those obtained by subjecting these to etherification, esterification, oxidation, acid treatment and the like. These raw material starches are roasted and dextrinized by a conventional method. Good. The degree of hydrolysis of roasted dextrin (hereinafter abbreviated as DE. DE is a value obtained by converting a reducing sugar value into dextrose) of 0.5 to 10 can be used.

There are various CGT-ases depending on their origins. macerans, B .; megat
erium, B. stearothernophilu
CGT-ase such as s can be used.

CGT-ase is added to the above-mentioned 4-20% aqueous solution of roasted dextrin in an amount of 5-1 to roasted dextrin.
After adding 50 units and reacting at pH 5 to 9 at a temperature of 30 to 60 ° C. for 10 to 160 hours, branched dextrin is produced. Ethanol was added to the reaction mixture for roasting dextrin.
Addition of 0 to 60% increases the production rate of branched CD. C
Although GT-ase is used in the form of powder or aqueous solution,
CGT-ase immobilized by a usual method can also be used.

After completion of the reaction, the reaction solution is decolorized and purified by a conventional method, concentrated and then dried using a spray dryer, a drum dryer or the like to obtain a branched CD-rich dextrin-containing powder. Branched CD can be purified from the above reaction mixture by a conventional method to obtain highly pure branched CD.

[0010]

As described above, according to the present invention, since roasted dextrin having a highly branched molecular structure is used as a raw material, C
The yield of branched CD produced by the action of GT-ase is increased.

[0011]

[Examples] The present invention will be specifically described below with reference to Examples, but the present invention is not limited thereto. Example 1 40 g of roasted dextrin of potato starch (DE4.6) was dissolved in 400 ml of water, and the pH was adjusted to 6.5 using 0.1N sodium hydroxide aqueous solution, and contizyme (B. macerans manufactured by Amano Pharmaceutical Co., Ltd.). CGT-ase, 6
(00 unit / ml) was added and reacted at 40 ° C. for 1 hour, then ethanol 24 ml was added and reacted at 40 ° C. for 48 hours. After the reaction, deactivate with acid, decolorize, and
After being concentrated, the dextrin having a high content of branched CD was obtained in the form of syrup. The yield of branched CD was determined by treating the above reaction solution with α-glucosidase and quantifying the remaining branched CD and CD using high performance liquid chromatography. Comparative Example 1 A dextrin syrup was obtained in the same manner as in Example 1 except that enzyme-modified dextrin was used instead of roasted dextrin. The yield of branched CD was measured in the same manner as in Example 1 and is shown in Table 1.

[Table 1]

[0012]

[Effect] As is clear from Table 1, according to the present invention, branched CD can be obtained in high yield from inexpensive roasted dextrin as a raw material without using expensive branched dextrin.

Claims (1)

[Claims] 1. A method for producing a branched cyclodextrin, which comprises reacting roasted dextrin with cyclodextrin glucanotransferase.