JPH0532701A - Water-soluble polysaccharides - Google Patents
Water-soluble polysaccharidesInfo
- Publication number
- JPH0532701A JPH0532701A JP3216241A JP21624191A JPH0532701A JP H0532701 A JPH0532701 A JP H0532701A JP 3216241 A JP3216241 A JP 3216241A JP 21624191 A JP21624191 A JP 21624191A JP H0532701 A JPH0532701 A JP H0532701A
- Authority
- JP
- Japan
- Prior art keywords
- water
- soluble polysaccharide
- film
- soluble
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- General Preparation And Processing Of Foods (AREA)
- Jellies, Jams, And Syrups (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は高分子でありながら水溶
性の多糖類に関する。更に詳しくは接着性、膜形成性、
膜の抗張性、増粘性（特にアルカリ域で増粘するが酸性
に戻すと粘度も下がる性質を有する）等に優れる水溶性
の多糖類を提供するものである。FIELD OF THE INVENTION The present invention relates to a polymeric but water-soluble polysaccharide. More specifically, adhesiveness, film forming property,
It is intended to provide a water-soluble polysaccharide which is excellent in film tensile strength, thickening property (in particular, it has a property of thickening in an alkaline region but decreasing in viscosity when returned to acidic).
【０００２】[0002]
【従来の技術】多糖類は多く知られ．なかでも水溶性の
多糖類は通常数千万〜千程度の広い分子量域に渡るが、
低分子すぎると増粘性、乳化安定性、膜形成性が失わ
れ、高分子すぎると高濃度溶液が得られ難い欠点を有す
る。一方、大豆の皮や細胞膜に由来する多糖類（所謂オ
カラ）やそのアルカリ分解物、酵素分解物も知られてい
るが、冷水に溶けにくく、又、一部学術的には水溶性画
分を取り出して糖組成等を研究しているが、分解法が本
発明に用いる方法と異なり、得られる画分の構造も異な
る。例えば、大豆のアルカリ分解によって得られる多糖
類は分子量が低く、本発明の水溶性多糖類のような優
れた結着材性、膜形成性、膜の抗張性がない、多糖類
の構造も、例えば比旋光度が異なる等本発明のものとは
異なる。又、収量も少なく工業的に不向きである。BACKGROUND OF THE INVENTION Many polysaccharides are known. Among them, water-soluble polysaccharides usually have a wide molecular weight range of tens of millions to 1,000s.
If the molecular weight is too low, the viscosity increase, emulsion stability, and film-forming property are lost. If the molecular weight is too high, a high-concentration solution is difficult to obtain. On the other hand, polysaccharides derived from soybean hulls and cell membranes (so-called okara) and their alkaline and enzymatic degradation products are also known, but are difficult to dissolve in cold water, and some academically Although the sugar composition is taken out and studied, the decomposition method is different from the method used in the present invention, and the structure of the obtained fraction is also different. For example, the polysaccharide obtained by alkaline decomposition of soybean has a low molecular weight, and has excellent binding material properties such as the water-soluble polysaccharide of the present invention, film-forming property, no film tensile property, and a polysaccharide structure. , Different from that of the present invention, for example, different in specific rotation. In addition, the yield is low and it is not industrially suitable.
【０００３】[0003]
【発明が解決しようとする問題点】本発明者等は天然多
糖類のなかで、水溶性で接着性に優れ且つ膜形成性を有
する安価なものは少なないことに気付いた。そこで、本
発明者等は高分子でありながら水溶性で、且つ膜形成性
や強い接着性（プルランに匹敵するか或いは凌駕する）
を有する多糖類を目的とした。DISCLOSURE OF THE INVENTION The present inventors have found that among natural polysaccharides, there are many inexpensive polysaccharides which are water-soluble, have excellent adhesiveness, and have film-forming properties. Therefore, the inventors of the present invention are water-soluble despite being a polymer, and have film-forming property and strong adhesiveness (comparable to or superior to pullulan).
Aimed at polysaccharides with.
【０００４】[0004]
【発明の構成】本発明者等は公知の多糖類を研究したが
水溶性の多糖類は膜形成性や接着性を有するものは究め
て少なく、反面、高分子の多糖類はほとんどが高濃度溶
液が困難であることから鋭意研究するなかで、入手の容
易なオカラ（水不溶性多糖類）を特定の条件で分解し、
分画・精製して目的とする水溶性多糖類を得ることに成
功し、本発明を完成するに到った。即ち、本発明は、構
成糖がラムノース、フコース、アラビノース、キシロー
ス、ガラクトース、グルコース及びウロン酸を含み、標
準プルラン（昭和電工（株）販）を用い、0.1 Ｍの硝酸
ナトリウム溶液中の粘度を測定する極限粘度法を用いて
測定した平均分子量が５〜１００万、比旋光度（２５
℃）が１５以上、水溶性で、接着性を有し、膜形成性を
有する水溶性多糖類である。DETAILED DESCRIPTION OF THE INVENTION The present inventors have studied known polysaccharides, but few water-soluble polysaccharides have film-forming properties and adhesive properties, while high-molecular polysaccharides are mostly high in concentration. In the course of diligent research because the solution is difficult, we decompose easily available okara (water-insoluble polysaccharide) under specific conditions,
The present invention has been completed by succeeding in obtaining the desired water-soluble polysaccharide by fractionation and purification. That is, in the present invention, the constituent sugars include rhamnose, fucose, arabinose, xylose, galactose, glucose and uronic acid, and a standard pullulan (available from Showa Denko KK) was used to measure the viscosity in a 0.1 M sodium nitrate solution. The average molecular weight measured using the limiting viscosity method is 5 to 1,000,000, and the specific optical rotation (25
It is a water-soluble polysaccharide having a temperature of 15 ° C. or higher, water solubility, adhesiveness, and film forming property.
【０００５】本発明の水溶性多糖類の構成糖はラムノー
ス、フコース、アラビノース、キシロース、ガラクトー
ス、グルコース及びウロン酸を含み、その他マンノー
ス、フラクトースを含むこともできる。その組成は特定
されるわけではないが、通常、ラムノース１〜４重量
％、フコース２〜８重量％、アラビノース１５〜５０重
量％、キシロース４〜１０重量％、ガラクトース２５〜
５０重量％、グルコース４重量％以下、ウロン酸１５〜
２５重量％が適当である。かかる組成の水溶性多糖類が
目的の物性に好適である。又、本発明の水溶性多糖類
は、ＨＰＬＣによるゲルろ過クロマト（東ソーG5000PWL
X 、溶離液：pH6.8 ,0.1M リン酸緩衝液）で分画すると
分子量によりＡ画分、Ｂ画分及びＣ画分から構成され
る。Ａ画分は高分子であり、増粘性、結着材、膜形成性
に優れ、Ｂ画分及びＣ画分は比較的低分子であり、増粘
性、結着材、膜形成性が減少する。The constituent sugars of the water-soluble polysaccharide of the present invention include rhamnose, fucose, arabinose, xylose, galactose, glucose and uronic acid, and may also contain mannose and fructose. Although its composition is not specified, it is usually 1 to 4% by weight of rhamnose, 2 to 8% by weight of fucose, 15 to 50% by weight of arabinose, 4 to 10% by weight of xylose, 25 to 25% of galactose.
50% by weight, glucose 4% by weight or less, uronic acid 15-
25% by weight is suitable. The water-soluble polysaccharide having such a composition is suitable for the desired physical properties. Further, the water-soluble polysaccharide of the present invention is subjected to gel filtration chromatography by HPLC (Tosoh G5000PWL
X, eluent: pH 6.8, 0.1 M phosphate buffer), it is composed of A fraction, B fraction and C fraction depending on the molecular weight. Fraction A is a polymer and is excellent in thickening property, binder and film forming property, and B and C fractions are relatively low molecules, and viscosity increasing property, binding material and film forming property are reduced. ..
【０００６】本発明の水溶性多糖類は、このＡ画分、Ｂ
画分及びＣ画分から構成されることにより増粘性が抑え
られ結着材、膜形成性に優れたものとなる。水溶性多糖
類Ａ画分及びＢ画分の糖組成はウロン酸、ラムノース、
フコース、アラビノース、キシロース、ガラクトース、
グルコースからなり、この糖組成中キシロースが１０重
量％以下が好適である。Ａ画分の糖組成と公知の水溶性
多糖類の糖組成を比較すると、例えばプルランがグルコ
ースを主成分とするポリマーであったり、アラビアガム
がフコースやキシロースを含まない多糖類であるのに比
べ、組成糖の種類は比較的多く、アラビノースとガラク
トースが主体でキシロースの占める割合も多く（組成糖
の１０重量％以上）、又、ラムノース、フコース、グル
コースの占める割合が比較的少ないところに特徴を有す
る。Ｃ画分の糖組成は、グルコースを含有せず、ウロン
酸、フコース、アラビノース、キシロース、ガラクトー
スからなるアラビノース主体の多糖類である。又、Ｃ画
分の糖組成にラムノースを含有する場合もある。The water-soluble polysaccharide of the present invention comprises the fraction A, the fraction B
By being composed of the fraction and the C fraction, the viscosity increase is suppressed and the binder and the film formability are excellent. The sugar composition of the water-soluble polysaccharides A fraction and B fraction is uronic acid, rhamnose,
Fucose, arabinose, xylose, galactose,
It is composed of glucose, and xylose in the sugar composition is preferably 10% by weight or less. Comparing the sugar composition of the A fraction with the sugar composition of known water-soluble polysaccharides, for example, pullulan is a polymer whose main component is glucose, and gum arabic is a polysaccharide that does not contain fucose or xylose. The types of sugar composition are relatively large, arabinose and galactose are the main components, and xylose accounts for a large proportion (10% by weight or more of the composition sugar), and rhamnose, fucose, and glucose account for a relatively small proportion. Have. The sugar composition of the C fraction is an arabinose-based polysaccharide composed of uronic acid, fucose, arabinose, xylose and galactose, which does not contain glucose. In addition, the sugar composition of the C fraction may contain rhamnose.
【０００７】尚、糖類の割合は次の分析法によるもので
ある。 ★ウロン酸の測定はBlumenkrantz法。 ★中性糖の測定はアルジト─ルアセテート法。 詳しい測定条件は実施例に記載している。又、分画成分
の重量割合がＡ＞Ｂ，Ａ＞Ｃであるほど低分子成分が少
なくなり水溶性でありながら目的とする接着性、膜形成
性に優れたものとなり好ましい。本発明の水溶性多糖類
の平均分子量は測定方法により異なるが、次記（Ａ法）
で測定した値が５〜１００万（好ましくは１０〜３０
万）、次記（Ｂ法）で測定した値が、平均分子量が３０
〜１２０万の水溶性多糖類Ａと３０〜１２０万の水溶性
多糖類Ａと平均分子量７〜３０万の水溶性多多糖類Ｂと
平均分子量３〜７万の水溶性多多糖類Ｃとを主成分とす
るものである。（Ａ法）標準プルラン（昭和電工（株）
販）を用い、0.1 Ｍの硝酸ナトリウム溶液での粘度を測
定する極限粘度法を用いて分子量を求めた。（Ｂ法）
標準プルラン（昭和電工（株）販）を用い、ＨＰＬＣに
よるゲルろ過クロマト（東ソーG5000PWLX 、溶離液：pH
6.8 ,0.1Mリン酸緩衝液）の保持時間から標準曲線を作
成し、試料の保持時間から分子量を求めた。The saccharide ratio is determined by the following analytical method. ★ Blumenkrantz method is used to measure uronic acid. ★ The neutral sugar is measured by the alditol acetate method. Detailed measurement conditions are described in Examples. Further, it is preferable that the weight ratios of the fractionated components are A> B and A> C, since the low molecular weight components are reduced and the water-soluble property is achieved, and the desired adhesiveness and film forming property are excellent. The average molecular weight of the water-soluble polysaccharide of the present invention varies depending on the measuring method.
The value measured by 5 to 1,000,000 (preferably 10 to 30)
10,000), the value measured by the following method (method B) is 30
-1.2 million water-soluble polysaccharide A, 300-1.2 million water-soluble polysaccharide A, water-soluble polysaccharide B having an average molecular weight of 70-300,000 and water-soluble polysaccharide C having an average molecular weight of 30,000-70,000 as main components It is what (Method A) Standard pullulan (Showa Denko KK)
Molecular weight was determined by an intrinsic viscosity method in which the viscosity in a 0.1 M sodium nitrate solution was measured. (Method B)
Using standard pullulan (sold by Showa Denko KK), gel filtration chromatography by HPLC (Tosoh G5000PWLX, eluent: pH
A standard curve was prepared from the retention times of 6.8 and 0.1 M phosphate buffer, and the molecular weight was determined from the retention times of the samples.
【０００８】本発明の水溶性多糖類の比旋光度（２５
℃）は１５以上、好ましくは３０〜７０が適当である。
後に製造法を説明するように、本発明の多糖類は酸分解
により得られるものであり、従来の方法であるアルカリ
分解によって得られる多糖類と糖組成は若干の相違しか
なくてもその構造及び分子量は異なる。この構造（比旋
光度により指標される）及び分子量の違いが、本発明の
水溶性多糖類を接着性及び膜形成性に優れたものとす
る。本発明の水溶性多糖類は水溶性である。本発明の水
溶性多糖類は接着性に優れかつ膜形成性にも優れる。
又、本発明の水溶性多糖類は蛋白質含量が１２重量％以
下、好ましくは８重量％以下が適当である。又、本発明
の水溶性多糖類は灰分含量が１〜８重量％、好ましくは
２〜７重量％が適当である。灰分の存在により水に対す
る溶解性が増加する。又、本発明の水溶性多糖類は食物
繊維として分析される含量５５重量％以上が適当であ
る。Specific rotation of the water-soluble polysaccharide of the present invention (25
(° C) is 15 or more, preferably 30 to 70 is suitable.
As will be described later in the production method, the polysaccharide of the present invention is obtained by acid decomposition, and the polysaccharide and sugar composition obtained by alkali decomposition, which is a conventional method, have a structure and a sugar composition which are slightly different from each other. The molecular weight is different. This difference in structure (indexed by specific optical rotation) and molecular weight makes the water-soluble polysaccharide of the present invention excellent in adhesiveness and film forming property. The water-soluble polysaccharide of the present invention is water-soluble. The water-soluble polysaccharide of the present invention is excellent in adhesiveness and film forming property.
The water-soluble polysaccharide of the present invention has a protein content of 12% by weight or less, preferably 8% by weight or less. The water-soluble polysaccharide of the present invention has an ash content of 1 to 8% by weight, preferably 2 to 7% by weight. The presence of ash increases the solubility in water. Further, the content of the water-soluble polysaccharide of the present invention is 55% by weight or more, which is analyzed as dietary fiber.
【０００９】以下実施例により本発明の実施態様を説明
する。 実施例１ （製造法）分離大豆蛋白製造工程で得られた生オカラに
２倍重量部の加水をし、塩酸にてｐＨを４．５に調製
し、１２０℃で１．５時間加水分解し、冷却後遠心分離
（10000g×30分間) し、上澄と沈澱部に分離し、沈澱部
を更に等重量の水で水洗し遠心分離して得た上澄と前記
上澄とを一緒にし、活性炭カラム処理をして得た液を乾
燥して水溶性多糖類（イ）を得た。一方、同様にして活
性炭カラム処理なしでも同様に水溶性多糖類（ロ）を得
た。The embodiments of the present invention will be described below with reference to examples. Example 1 (Production method) The raw okara obtained in the isolated soybean protein production step was added with 2 parts by weight of water, adjusted to pH 4.5 with hydrochloric acid, and hydrolyzed at 120 ° C. for 1.5 hours. After cooling, centrifugation (10000 g × 30 minutes) was performed to separate a supernatant and a precipitate, and the precipitate was washed with water of an equal weight and centrifuged to combine the supernatant with the supernatant, The liquid obtained by treating with an activated carbon column was dried to obtain a water-soluble polysaccharide (a). On the other hand, in the same manner, a water-soluble polysaccharide (b) was similarly obtained without the activated carbon column treatment.
【００１０】[0010]
【表１】 組成割合 ─────────────────────────── 成 分 イ ロ ─────────────────────────── 水分 5.71 5.10 粗蛋白 1.93 5.43 粗灰分 5.29 5.30 多糖類 87.07 84.17 旋光度（２５℃） ＋54.3 ＋37.4 ─────────────────────────── 活性炭処理によって色素成分や疎水性成分、低分子成分
も除かれた。次に、（イ）及び（ロ）の水溶性多糖類の
糖組成を次の方法で分析した。[Table 1] Composition ratio ─────────────────────────── Component yellow ────────────── ────────────── Moisture 5.71 5.10 Crude protein 1.93 5.43 Crude ash 5.29 5.30 Polysaccharide 87.07 84.17 Optical rotation (25 ° C) +54.3 +37.4 ────────── ────────────────── The pigment component, hydrophobic component, and low-molecular component were also removed by the activated carbon treatment. Next, the sugar composition of the water-soluble polysaccharides (a) and (b) was analyzed by the following method.
【００１１】[0011]
【表２】 [Table 2]
【００１２】実施例２ 分離大豆蛋白製造工程で得られた生オカラに２倍重量部
の加水をし、塩酸にてｐＨを２．５に調製し、１００℃
で１．５時間加水分解し、冷却後中和し遠心分離（1000
0g×30分間) し、上澄と沈澱部に分離し、沈澱部を更に
等重量の水で水洗し遠心分離して得た上澄と前記上澄と
を一緒にし、乾物と同重量部の活性炭で処理をして得た
液を乾燥して水溶性多糖類を得た。この水溶性多糖類
は、水分４．１％、粗蛋白２．７％、粗灰分４．２％で
あった。次にカラム（セファクリルＳ４００: pharmaci
a 製）を用い、溶離液として０．１Ｍリン酸緩衝液（ｐ
Ｈ６．８）を用い、３つの多糖類ピーク（Ａ、Ｂ、Ｃと
する）を分取した。Ａ、Ｂ及びＣの割合は６５；１５；
２０であった。実施例１と同様にして糖組成を測定した
結果を表−３に記す。Example 2 The raw okara obtained in the isolated soybean protein production process was mixed with 2 parts by weight of water, adjusted to pH 2.5 with hydrochloric acid, and heated to 100 ° C.
Hydrolyzed for 1.5 hours, cooled, neutralized and centrifuged (1000
(0 g × 30 minutes), separate the supernatant and the precipitate, wash the precipitate with an equal weight of water and centrifuge to obtain the supernatant, and combine the supernatant with the same weight of dry matter. The liquid obtained by treating with activated carbon was dried to obtain a water-soluble polysaccharide. This water-soluble polysaccharide had a water content of 4.1%, a crude protein of 2.7% and a crude ash content of 4.2%. Next, the column (Sephacryl S400: pharmaci
a) and 0.1 M phosphate buffer (p
H6.8) was used to separate three polysaccharide peaks (designated as A, B, and C). The ratio of A, B and C is 65; 15;
It was 20. The results of measuring the sugar composition in the same manner as in Example 1 are shown in Table 3.
【００１３】[0013]
【表３】 糖組成（重量％） ─────────────────────────────── 糖の種類 分画前 Ａ Ｂ Ｃ ─────────────────────────────── ウロン酸 19.6 23.5 26.0 9.6 ラムノース 2.1 2.5 2.5 0 フコース 4.1 4.0 3.2 10.3 アラビノース 25.8 17.7 21.0 60.0 キシロース 5.9 7.0 5.8 4.7 ガラクトース 40.4 41.4 38.5 13.9 グルコース 2.1 1.8 2.2 0 ─────────────────────────────── 次に、Ａ画分をゲルろ過クロマト（Sephadex G-25)）処
理して脱塩して、灰分をＡ画分の乾燥固形分中0.8 重量
％にした凍結乾燥Ａ画分を得た。これを、水に溶かそう
としたが溶けなかった。[Table 3] Sugar composition (% by weight) ─────────────────────────────── Sugar type Before fractionation ABC ─────────────────────────────── Uronic acid 19.6 23.5 26.0 9.6 Rhamnose 2.1 2.5 2.5 0 Fucose 4.1 4.0 3.2 10.3 Arabinose 25.8 17.7 21.0 60.0 Xylose 5.9 7.0 5.8 4.7 Galactose 40.4 41.4 38.5 13.9 Glucose 2.1 1.8 2.2 0 ─────────────────────────────── Next The A fraction was subjected to gel filtration chromatography (Sephadex G-25) for desalting to obtain a freeze-dried A fraction in which the ash content was 0.8% by weight in the dry solid content of the A fraction. I tried to dissolve it in water but it didn't.
【００１４】実験例３ 実験例１と同様にして得たイを、ＪＩＳ規格Ｋ６８４８
−1987及びＫ６８５１−1976に準じてけやきを用いて接
着強度試験を行った。加熱圧締は行わず、接着後２０℃
・ＲＨ６０％・４８時間乾燥後にレオナー（山電（株）
製）にて引っ張り専断強度を測定した。結果を表─４
に示す。Experimental Example 3 A obtained in the same manner as in Experimental Example 1 was subjected to JIS K6848.
-Adhesion strength test was performed using Keyaki according to -1987 and K6851-1976. 20 ° C after bonding without heating and pressing
・ RH 60% ・ Leoner after drying for 48 hours (Yamaden Co., Ltd.)
Tensile strength. The results are shown in Table-4.
Shown in.
【００１５】[0015]
【表４】 表─４ ────────────────────────── 接着強度 ( kgf ／平方cm ) ────────────────────────── イ 56.4 ± 12.4 プルラン 40.5 ± 15.7 アラビアガム 30.7 ± 10.8 ─────────────────────────── 実施例１で得られた水溶性多糖類イの引っ張り強度が強
いこと、即ち接着性に優れることがが分かった。[Table 4] Table -4 ────────────────────────── Adhesive strength (kgf / cm2) ───────── ───────────────── a 56.4 ± 12.4 pullulan 40.5 ± 15.7 gum arabic 30.7 ± 10.8 ──────────────────── It was found that the water-soluble polysaccharide B obtained in Example 1 has a high tensile strength, that is, excellent adhesiveness.
【００１６】実験例４ 実験例１と同様にして得たイの１５％水溶液を調製し、
合成フィルム上に薄く展延しながら６０℃にて乾燥して
合成フィルムから剥離して膜を製造し実験例３と同様に
してレオナーを用いて膜の抗張力を測定した。結果を表
─５ に示す。Experimental Example 4 A 15% aqueous solution of B obtained in the same manner as in Experimental Example 1 was prepared,
A thin film was spread on a synthetic film, dried at 60 ° C., peeled from the synthetic film to produce a film, and the tensile strength of the film was measured using a Leoner in the same manner as in Experimental Example 3. The results are shown in Table-5.
【００１７】[0017]
【表５】 表─５ ───────────────────────────────── 抗張力 ( kgf ／平方cm ) ───────────────────────────────── イ 552.1 ± 72.0 プルラン 509.4 ± 207.3 アラビアガム ─── ──── ───────────────────────────────── アラビアガムは膜を形成せず測定できなかった。しか
し、水溶性多糖類イは強い膜を形成した。[Table 5] Table -5 ───────────────────────────────── Tensile strength (kgf / cm2) ─── ────────────────────────────── ─ 552.1 ± 72.0 Pullulan 509.4 ± 207.3 Gum Arabic ─── ──── ─── ────────────────────────────── Gum arabic could not be measured because it did not form a film. However, water-soluble polysaccharide B formed a strong film.
【００１８】実験例５ 実験例１と同様にして得たイ及びロを１０％溶液とな
し、３０°Ｃにおける粘度をRheomat 115 ( Contraves
社製）を用いて測定した。尚、比較として市販プルラン
（PF-20)及びアラビアガムも１０％溶液となし、３０°
Ｃにおける粘度をRheomat 115 ( Contraves 社製）を用
いて測定した。結果を図１に示す。Experimental Example 5 A and B obtained in the same manner as in Experimental Example 1 were made into 10% solutions, and the viscosity at 30 ° C. was Rheomat 115 (Contraves).
(Manufactured by the company). For comparison, commercially available pullulan (PF-20) and gum arabic were also made into a 10% solution, and 30 °
The viscosity at C was measured using Rheomat 115 (manufactured by Contraves). The results are shown in Figure 1.
【００１９】実験例６ 実験例５と同様にして調製したイ及びロの１０％溶液に
塩化カルシウムを加えて、実験例５と同様にして粘度に
及ぼす影響を調べた。結果を図２に示す。カルシウムイ
オンに影響されないことが分かった。Experimental Example 6 Calcium chloride was added to a 10% solution of a and b prepared in the same manner as in Experimental Example 5, and the effect on viscosity was examined in the same manner as in Experimental Example 5. The results are shown in Figure 2. It was found that it was not affected by calcium ions.
【００２０】実験例７ 実験例５と同様にして粘度を測定するに当たり、溶液の
ｐＨを変化させてみた。結果を図３に示す。プルランや
アラビアガムと異なり、アルカリ域で粘度が上昇する特
徴を示した。又、ｐＨをもとに戻すと粘度のもとに戻る
性質を示した。Experimental Example 7 In measuring the viscosity in the same manner as in Experimental Example 5, the pH of the solution was changed. Results are shown in FIG. Unlike pullulan and gum arabic, it showed a characteristic that viscosity increased in the alkaline range. It also showed the property of returning to the original viscosity when the pH was returned to the original value.
【００２１】[0021]
【発明の効果】以上説明したように、下記本発明により
高平均分子量にもかかわらず水溶性を有し、接着力、抗
張力に優れる多糖類が可能になったものである。Industrial Applicability As described above, according to the present invention described below, a polysaccharide having a high average molecular weight, water solubility, and excellent adhesive strength and tensile strength has been made possible.
【図１】実験例５における粘度を示す。中空きの四角印
は（ロ）を、中空きの丸印は（イ）を、中塗りの四角印
はプルランを、中塗り丸印はアラビアガムを示す。1 shows the viscosity in Experimental Example 5. The open squares indicate (b), the open circles indicate (a), the open squares indicate pullulan, and the open circles indicate gum arabic.
【図２】実験例６における粘度を示す。中空きの四角印
は（ロ）を、中空きの丸印は（イ）を、中塗りの四角印
はプルランを、中塗り丸印はアラビアガムを示す。FIG. 2 shows the viscosity in Experimental Example 6. The open squares indicate (b), the open circles indicate (a), the open squares indicate pullulan, and the open circles indicate gum arabic.
【図３】実験例７における粘度を示す。中空きの四角印
は（ロ）を、中空きの丸印は（イ）を、中塗りの四角印
はプルランを、中塗り丸印はアラビアガムを示す。FIG. 3 shows the viscosity in Experimental Example 7. The open squares indicate (b), the open circles indicate (a), the open squares indicate pullulan, and the open circles indicate gum arabic.
Claims (1)
ース、キシロース、ガラクトース、グルコース及びウロ
ン酸を含み、標準プルラン（昭和電工（株）販）を用
い、0.1 Ｍの硝酸ナトリウム溶液中の粘度を測定する極
限粘度法を用いて測定した平均分子量が５〜１００万、
比旋光度（２５℃）が１５以上、水溶性で、接着性を有
し、膜形成性を有する水溶性多糖類。Claims: 1. The constituent sugars include rhamnose, fucose, arabinose, xylose, galactose, glucose and uronic acid, and 0.1 M sodium nitrate solution using standard pullulan (available from Showa Denko KK) The average molecular weight measured using the intrinsic viscosity method for measuring the viscosity of the medium is 5 to 1,000,000,
A water-soluble polysaccharide having a specific optical rotation (25 ° C.) of 15 or more, being water-soluble, having adhesive properties and having film-forming properties.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3216241A JP3018622B2 (en) | 1991-08-01 | 1991-08-01 | Water-soluble polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP3216241A JP3018622B2 (en) | 1991-08-01 | 1991-08-01 | Water-soluble polysaccharide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0532701A true JPH0532701A (en) | 1993-02-09 |
| JP3018622B2 JP3018622B2 (en) | 2000-03-13 |
Family
ID=16685489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP3216241A Expired - Lifetime JP3018622B2 (en) | 1991-08-01 | 1991-08-01 | Water-soluble polysaccharide |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3018622B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2492338A1 (en) | 2011-02-24 | 2012-08-29 | Fujifilm Corporation | Plate cleaning agent for lithographic printing plate and plate surface treatment method for lithographic printing plate |
| CN114907496A (en) * | 2022-06-22 | 2022-08-16 | 常熟理工学院 | Fig leaf polysaccharide and preparation method and application thereof |
-
1991
- 1991-08-01 JP JP3216241A patent/JP3018622B2/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2492338A1 (en) | 2011-02-24 | 2012-08-29 | Fujifilm Corporation | Plate cleaning agent for lithographic printing plate and plate surface treatment method for lithographic printing plate |
| CN114907496A (en) * | 2022-06-22 | 2022-08-16 | 常熟理工学院 | Fig leaf polysaccharide and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JP3018622B2 (en) | 2000-03-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2882171B2 (en) | Water-soluble polysaccharide and method for producing the same | |
| Ma et al. | Comparison of citrus pectin and apple pectin in conjugation with soy protein isolate (SPI) under controlled dry-heating conditions | |
| Fedeniuk et al. | Composition and physicochemical properties of linseed (Linum usitatissimum L.) mucilage | |
| Guillotin et al. | Populations having different GalA blocks characteristics are present in commercial pectins which are chemically similar but have different functionalities | |
| JP2772162B2 (en) | Improved polydextrose composition | |
| Zhang et al. | Fractionation, partial characterization and bioactivity of water-soluble polysaccharides and polysaccharide–protein complexes from Pleurotus geesteranus | |
| Liu et al. | Emulsifying and structural properties of polysaccharides extracted from Chinese yam by an enzyme-assisted method | |
| FR2584728A1 (en) | PROCESS FOR THE SULFATION OF GLYCOSAMINOGLYCANS AND THEIR FRAGMENTS | |
| KR102520754B1 (en) | Polypentoic acid, pharmaceutical composition and anticoagulant | |
| Steinhorn et al. | Isolation and characterisation of arabinogalactan-proteins from New Zealand kanuka honey | |
| WO2019061018A1 (en) | Method for extracting pectin rich in rg-i | |
| Capek et al. | Polysaccharides from the roots of the marsh mallow (Althaea officinalis L., var. Rhobusta): Structural features of an acidic polysaccharide | |
| Ben Amor et al. | Efficient extraction of a high molecular weight ulvan from stranded Ulva sp. biomass: Application on the active biomembrane synthesis | |
| EP0475383B1 (en) | Polysaccharide composition or polysaccharide having heparinoid activity, process for producing the same, and anticoagulant containing the same as active ingredient | |
| Lee et al. | Physicochemical properties of β-glucan from acid hydrolyzed barley | |
| JPH0532701A (en) | Water-soluble polysaccharides | |
| Ebermann et al. | Fractionation of starch by gel filtration on agarose beads | |
| Kaye et al. | Chemistry of tissues. 4. Methylation studies on hyaluronic acid | |
| KR100200547B1 (en) | Method of separation and purification for low molecular weight chitosan using multi-step membrane process | |
| JPS5945301A (en) | Polysaccharide and its preparation | |
| Hundie et al. | Extraction and characterization of pectin from lemon waste for commercial applications | |
| CA2988828C (en) | Enzymatic-assisted hydrothermal extraction of hemicelluloses | |
| Whistler et al. | Preparation and characterization of 6-amino-6-deoxyamylose | |
| JP2533237B2 (en) | Glue | |
| Godshall | Interference of plant polysaccharides and tannin in the Coomassie Blue G250 test for protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20090107 Year of fee payment: 9 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20100107 Year of fee payment: 10 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110107 Year of fee payment: 11 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20110107 Year of fee payment: 11 |
|
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120107 Year of fee payment: 12 |
|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Free format text: PAYMENT UNTIL: 20120107 Year of fee payment: 12 |