JPH05307039A - Antibody sensing method - Google Patents

Abstract

PURPOSE:To establish effective sensing of antibody by performing processing of the other bodies which has high order of priority and is capable of being processed in the wait time after a elapse of the processing time at each processing stage of a body to be inspected. CONSTITUTION:The processing procedures for sensing are set at each processing stage together with the processing time necessary and the wait time, and the order of priority of the sensing process is set between the bodies to be inspected A, B, C. For example, the bodies A1-3, B1-3, C1-3 are selected according to the set orders of priority A>B>C and time of waiting (t), and the sensing process is started with the body, A1, which has the highest order of priority. After elapse of the processing time at this processing stage, the processing is made in the time of waiting Alt, wherein that of the other bodies B, C at the processing stage capable of performing processing with the wait time being zero or having gone, which has high order of priority, i.e., the body B1, is subjected to this processing.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antibody detection method using an automatic antibody detection device.

[0002]

2. Description of the Related Art Conventional antibody detection methods include, for example, radioimmunoassay (radioimmunoassay: RIA method) and enzyme immunoassay (enzyme immunoassay: ELIS).
A method) etc. are known. In these immunoassays, the antibody or antigen is identified and quantified by utilizing the high specificity and detection sensitivity of the antigen-antibody reaction.

The RIA method requires a safety measure against exposure to radiation and a special facility because the secondary antibody is labeled with a radioactive substance, and the storage stability of the labeled antibody is low. Therefore, at present, the ELISA method is mainly used. Has been.

There are many kinds of ELISA methods. For example, taking solid-phase ELISA used for detection and quantification of monoclonal antibody as an example, (1) an object to be measured is an antigen which is immobilized on a solid phase in advance. Indirect method in which a monoclonal antibody to be reacted is reacted, and then an enzyme-labeled second antibody is reacted, (2) The complementary antibody is immobilized on a solid phase, and then a polyvalent antigen is added and allowed to bind to the measurement target. Sandwich method in which the following monoclonal antibody is reacted, and then the enzyme-labeled second antibody is reacted, (3) competition between immobilized antigen and free antigen, or competition between immobilized antigen and hapten substance Competitive ELISA method (Competitive indirect method,
Competitive sandwich method) etc.

As described above, although there are many kinds of ELISA methods, they basically consist of a processing procedure including a plurality of processing steps from the start to the end of the detection processing, and a series of processing is sequentially (sequentially). This is how to do it. Then, in each processing stage, it is necessary to perform a certain process and then leave it for a certain time (waiting time).

[0006] For example, taking the sandwich method as an example, the antibody detection process is usually carried out by the procedure shown in FIG. (A) First, one type of specific antibody 2 is placed in each assay hole of a solid support (for example, a 96-well polystyrene plate) 1.
Is dispensed and incubated for a certain period of time to adsorb the antibody on the surface of the solid phase, followed by washing.

(B) In order to block unadsorbed sites on the solid support (prevent nonspecific reaction),
A solution containing protein 3 irrelevant to the antibody is dispensed into each assay hole of the plate on which the specific antibody is immobilized, and the plate is incubated for a certain period of time and then washed.

(C) A solution containing polyvalent antigen 4 is dispensed into each assay hole, incubated for a certain period of time to react, and then washed. (D) As a sample, a liquid containing an antibody specific to an antigen [specific antibody derived from an animal species different from the species used for adsorption in (a) above] 5 is collected and dispensed into each assay hole, Incubate for a certain period of time to react, and then wash.

(E) An enzyme-labeled second antibody showing specificity to the antibody class to which the antibody specific to the above-mentioned antigen belongs [Antibodies specific to the animal species of the antibody used in (d): enzyme A solution containing labeled anti-immunoglobulin antibody] 6 is dispensed into each assay hole, incubated for a certain period of time to react, and then washed.

(F) An enzyme substrate solution containing the enzyme substrate and the indicator 7 is dispensed into each assay well and incubated for a certain period of time to react. (G) Absorbance is measured with a spectrophotometer (ELISA plate reader) or a colorimeter, and the second antibody is detected and quantified based on the intensity of color development to detect the presence or absence and the amount of the specific antibody that has reacted with the antigen.

As seen in this example, the antibody detection method is
It is composed of a processing procedure including a plurality of sequential processing steps from the detection processing start (a) to the end (g),
In each treatment stage, it is usually necessary to perform a certain treatment and then leave it for a certain period of time for incubation or the like. This waiting time is a waiting time, but incubation or reaction often takes a long time.

On the other hand, there is also known an antibody detection device that automates the antibody detection method as described above. Specifically, it is an antibody detection device having a reagent supply unit, a sample supply unit, a preparative dispensing unit, a transport unit, a measurement unit, a result output unit, and these control units.
However, even in such a conventional automation device, a series of processes similar to the above is merely performed sequentially.

Therefore, the conventional apparatus has drawbacks such that the waiting time cannot be effectively utilized, samples having different processing procedures cannot be mixed, and samples cannot be added during the processing operation.

[0014]

DISCLOSURE OF THE INVENTION An object of the present invention is to provide a method for automatically detecting an antibody using an automated antibody detection apparatus, by effectively utilizing the waiting time to process or process other samples. It is an object of the present invention to provide an antibody detection method capable of processing a sample having a different procedure or adding a sample during the processing operation.

As a result of earnest studies to overcome the above-mentioned problems of the prior art, the present inventor found that a series of processing procedures and processing time and waiting time required at each processing step were prepared in advance according to the type of sample. Is set, and priorities are set among the samples received for the detection process, and when performing the detection process of each sample, the sample to be processed is selected according to the set priority and waiting time. After the processing time in one processing stage of the sample has passed, during the waiting time, among the other samples in the processing stage that can be processed after the waiting time has passed, by processing the sample with high priority, They have found that they can be achieved, and have completed the present invention.

[0016]

Thus, according to the present invention, there is provided an antibody detection device having a reagent supply section, a sample supply section, a preparative dispensing section, a transfer section, a measurement section, a result output section, and a control section thereof. In the method of automatically detecting an antibody using (1) a treatment procedure including a plurality of treatment steps from the start to the end of the detection treatment depending on the type of the sample (antibody to be detected) , Setting the processing time and waiting time required in each processing stage, and (2) setting the priority of the detection processing among the received samples,

(3) When performing the detection process of each sample, the sample to be processed is selected according to the set priority and waiting time, the detection process is started from the sample with the highest priority, and one sample is processed. After the lapse of the processing time in the stage, during the waiting time, a sample having a high priority is processed among other samples in the processing stage where the waiting time is zero or the waiting time has passed and can be processed. A method for detecting an antibody is provided.

The present invention will be described in detail below. The antibody detection apparatus used in the present invention has a configuration example shown in the block diagram of FIG. 4, in which a reagent supply section, a sample supply section, a dispensing section, a transfer section, a measurement section, a result output section, and the like. It has a control unit. FIG. 5 is a perspective view of a configuration example of the antibody detection device. The antibody detection device of FIG. 5 includes a reagent supply unit 52, a sample supply unit 51, a dispensing unit 53, a transport unit 54,
Measurement unit 55, result output unit 57 and control unit 56 thereof
have.

FIG. 2 is an example of a flow chart of the antibody detection method according to the present invention. First, depending on the type of sample (antibody to be detected),
Set the processing procedure including multiple sequential processing steps from the start to the end of the detection processing together with the processing time and waiting time required for each processing step, and set the priority of the detection processing between the received samples. Is set and input to the control unit.

When performing the detection process for each sample, the priority among the samples is checked, and the detection process is started from the sample having the highest priority. The sample addition check determines whether or not a new sample is added, and if it is added, the type, processing procedure, processing time and waiting time at each processing stage, and priority input for that sample. Accept. For the sample being processed, it is checked whether the processing procedure is completed, and if there is the next processing stage, the priority and waiting time are checked.

In the priority and waiting time check, it is decided to select one of the specimens currently in the apparatus which is not in waiting time and execute the next processing step.
That is, during the waiting time of a certain sample, the sample having a high priority is processed among the other samples in the processing stage where the waiting time is zero or the waiting time has elapsed. Hereinafter, the detection process is performed for all the samples according to the same flow. FIG. 3 is an example of a time chart showing a processing flow of the antibody detection method according to the present invention.

[0022]

EXAMPLE An example of the antibody detection method of the present invention will be described with reference to FIGS. For example, in the case where there are three types of samples, A, B, and C, and the processing procedure, the processing time and waiting time in each processing stage, and the priority for these three types of samples are set as follows. explain.

(A) Treatment procedure of specimens A, B and C A: A ₁ , A ₂ , A ₃ , B: B ₁ , B ₂ , B ₃ , C: C ₁ , C ₂ , C ₃ . However, in FIG. 3, A ₁ , A _2, etc. also represent the processing time at each processing stage.

(B) Waiting time for each processing step A: A ₁ t, A ₂ t, A ₃ t, B: B ₁ t, B ₂ t, B ₃ t, C: C ₁ t , C ₂ t, C ₃ t. (C) Priority (Priority) A>B> C

According to the priority check of FIG. 2, the apparatus executes the process A ₁ on the sample A. After the end of execution, wait for waiting time A ₁ t. In the meantime, the apparatus performs the sample addition check, and executes the process B _{1 of the} sample B having the next highest priority by checking the priority and the reaction waiting time. After the end of execution, wait for waiting time B ₁ t. In the meantime, it is checked whether the waiting time A ₁ t or more has passed for the sample A, and if not, the process C ₁ is executed for the sample C.
If the time has passed, the process A ₂ is executed for the sample A according to the priority of A and C. Hereinafter, similarly, the priority and the waiting time are checked for each processing stage of each sample, and each processing is executed according to the priority.

As described above, the waiting time and priority of each sample are checked every time one processing step is completed, and the sample that can be processed is selected and executed. Other samples are processed in the process, and at the same time, samples with different processing procedures and waiting times can be processed.

Further, when a sample is added every time one process is completed and a sample is added, the processing procedure, the processing time and waiting time at each processing stage, the type,
It is also possible to set priorities and add new ones during the operation.

[0028]

EFFECTS OF THE INVENTION According to the present invention, when performing antibody detection using the automatic antibody detection device, the waiting time can be effectively utilized, various samples having different processing procedures can be mixed, and samples can be added during the processing operation. Because of this, it is efficient to use it for screening and research that processes a large amount of samples in fields such as biochemistry and pharmaceuticals.

[Brief description of drawings]

FIG. 1 is an explanatory diagram showing an example of a processing procedure of a general antibody detection method.

FIG. 2 is a flowchart showing an example of the procedure of the antibody detection method according to the present invention.

FIG. 3 is a time chart showing an example of the flow of the antibody detection method according to the present invention.

FIG. 4 is a block diagram showing a configuration example of an antibody detection device used in the present invention.

FIG. 5 is a perspective view showing a configuration example of an antibody detection device used in the present invention.

[Explanation of sign]

 1 solid support 2 specific antibody 3 protein unrelated to antigen 4 polyvalent antigen 5 antibody specific to antigen 6 enzyme-labeled anti-immunoglobulin antibody 7 enzyme substrate and indicator

Claims (1)

[Claims] 1. A reagent supply section, a sample supply section, a preparative dispensing section,
In a method for automatically detecting an antibody using an antibody detection device having a transport section, a measurement section, a result output section, and a control section thereof, (1) detection is performed according to the type of sample (antibody to be detected) A processing procedure including a plurality of sequential processing steps from the start to the end of processing is set together with the processing time and waiting time required in each processing step, and (2) detection processing is performed between the received samples. Setting the priority, (3) When performing the detection process for each sample, select the sample to be processed according to the set priority and waiting time, and start the detection process from the sample with the highest priority. After the processing time in one processing stage of the sample has elapsed, during the waiting time, the sample with the higher priority is processed among the other samples in the processing stage where the waiting time is zero or the waiting time has passed. A method for detecting an antibody, comprising: