JPH05281109A - Manufacture of specimen - Google Patents
Manufacture of specimenInfo
- Publication number
- JPH05281109A JPH05281109A JP8118192A JP8118192A JPH05281109A JP H05281109 A JPH05281109 A JP H05281109A JP 8118192 A JP8118192 A JP 8118192A JP 8118192 A JP8118192 A JP 8118192A JP H05281109 A JPH05281109 A JP H05281109A
- Authority
- JP
- Japan
- Prior art keywords
- adhesive tape
- adhesive
- skin
- cover glass
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、皮膚標本の作製方法に
関するものであり、詳しくは、皮膚表面形態を評価する
ための平面性、観察性のよい標本を作製する方法を提供
するものである。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for preparing a skin sample, and more particularly to a method for preparing a sample having good flatness and observability for evaluating the skin surface morphology. ..
【０００２】[0002]
【従来の技術】皮膚表面の微少な形態を評価し、その特
徴を的確に識別することは、美容、化粧などにとって皮
膚の性状や肌質を知る指標を得る上で、極めて重要なこ
とである。2. Description of the Related Art It is extremely important for cosmetics, makeup, etc. to evaluate the minute morphology of the skin surface and to accurately identify its characteristics, in order to obtain an index to know the properties and skin quality of the skin. ..
【０００３】従来、皮膚性状・肌質などを把握するため
の皮膚表面形態を知るための方法の１つとして、粘着テ
ープを皮膚表面に貼着、剥離して粘着テープの粘着面に
皮膚角質を移し取り、必要に応じて染色した後、顕微鏡
での観察性をよくするために、試料とカバーガラスとの
間に空気が入らないようにするためにシリコン材を封入
する方法が行われている。[0003] Conventionally, as one of the methods for knowing the skin surface morphology for grasping the skin properties and skin quality, etc., an adhesive tape is attached to the skin surface and peeled off, and keratin skin is attached to the adhesive surface of the adhesive tape. After transferring and staining if necessary, a method of encapsulating a silicon material to prevent air from entering between the sample and the cover glass is used in order to improve the observability under a microscope. ..
【０００４】[0004]
【発明が解決しようとする課題】しかしながら、粘着テ
ープを平らに保つために、カバーガラスの上から適当な
圧力を加える必要がある。However, in order to keep the pressure-sensitive adhesive tape flat, it is necessary to apply an appropriate pressure on the cover glass.
【０００５】そのために長時間を要し、さらに、カバー
ガラスを割らないようにするために相当の注意を必要と
するなど作業効率がよくない。また、このように注意し
てもシリコン材が完全に硬化しないために粘着テープの
しわがもとにもどり、出来上がった標本の平面性が悪い
ために、顕微鏡撮影の際にフォーカスを合わせるのに手
間取り、作業者の目に疲労が蓄積するなどの問題があっ
た。For this reason, it takes a long time, and in addition, it requires great care in order not to break the cover glass, resulting in poor work efficiency. In addition, even if you take such precautions, the silicon material will not completely cure, and the wrinkles of the adhesive tape will return to the original shape, and the flatness of the finished sample will be poor. However, there was a problem such as fatigue accumulated in the eyes of workers.
【０００６】本発明は、このような問題を解決するため
に、撮影する際にフォーカスを合わせやすいように平面
性、観察性にすぐれた標本を作製する方法であって、さ
らに、機械による作製の自動化が可能な簡便な方法を提
供することを課題とする。In order to solve such a problem, the present invention is a method for producing a specimen excellent in flatness and observability so that it can be easily focused during photographing. An object is to provide a simple method that can be automated.
【０００７】[0007]
【課題を解決するための手段】本発明者らは、前記課題
を解決するために鋭意研究を行った結果、粘着テープに
移し取った皮膚角質層を紫外線硬化材料で固定すること
により、迅速に、かつ、平面性、観察性のよい標本を作
製することができることを見出し、本発明に至った。Means for Solving the Problems As a result of intensive studies for solving the above-mentioned problems, the inventors of the present invention rapidly fixed the skin stratum corneum transferred to an adhesive tape with an ultraviolet curable material. Further, they have found that a sample having good flatness and observability can be produced, and have completed the present invention.
【０００８】以上のような経緯から本発明は皮膚標本の
作製用に開発されたが、観察対象を皮膚に限る必要はな
く、例えば毛髪、爪、動物や植物の組織などの観察にも
好適に使用できることが判明した。Although the present invention was developed for the preparation of skin specimens from the above background, it is not necessary to limit the observation object to the skin, and it is suitable for observation of hair, nails, tissues of animals or plants, etc. It turned out to be usable.
【０００９】よって、本発明は、樹脂フィルムを基材と
した粘着テープに観察対象物を貼着し、この粘着テープ
の粘着面に紫外線硬化性樹脂を塗布し、その上にカバー
ガラスをのせ、カバーガラス及び粘着テープを平坦面で
挟圧しつつ紫外線を照射して紫外線硬化性樹脂を硬化さ
せることを特徴とする標本の作製方法であるといえる。Therefore, according to the present invention, an object to be observed is attached to an adhesive tape having a resin film as a base material, an ultraviolet curable resin is applied to the adhesive surface of the adhesive tape, and a cover glass is placed on the adhesive surface. It can be said that the method for producing a specimen is characterized in that the cover glass and the pressure-sensitive adhesive tape are sandwiched between the flat surfaces to irradiate ultraviolet rays to cure the ultraviolet curable resin.
【００１０】さらに、樹脂フィルムを基材とした粘着テ
ープに観察対象物を貼着するとともに、窓孔を有するホ
ルダーを用意し、前記観察対象物を貼着した粘着テープ
の粘着面が前記窓孔に対応するように粘着テープをホル
ダーに貼着し、観察対象を粘着した粘着テープの粘着面
に紫外線硬化性樹脂を塗布し、その上にカバーガラスを
のせ、カバーガラス及び粘着テープを平坦面で挟圧しつ
つ紫外線を照射して紫外線硬化性樹脂を硬化させること
を特徴とする標本の作製方法である。Further, an object to be observed is adhered to an adhesive tape having a resin film as a base material, and a holder having a window hole is prepared, and the adhesive surface of the adhesive tape to which the object to be observed is adhered is the window hole. Adhesive tape is attached to the holder so that the observation target is adhered, UV-curable resin is applied to the adhesive surface of the adhesive tape, the cover glass is placed on it, and the cover glass and adhesive tape are placed on a flat surface. It is a method for producing a specimen, which comprises irradiating ultraviolet rays while pinching it to cure the ultraviolet curable resin.
【００１１】また本発明は、窓孔を有し、この窓孔に対
応するように樹脂フィルムを基材とした粘着テープを貼
着したホルダーを用意し、この粘着テープの粘着面に観
察対象物を貼着し、この粘着面に紫外線硬化性樹脂を塗
布し、その上にカバーガラスをのせ、カバーガラス及び
粘着テープを平坦面で挟圧しつつ紫外線を照射して紫外
線硬化性樹脂を硬化させることを特徴とする標本の作製
方法、及び、前記の各方法において、前記観察対象物が
皮膚角質であり、観察対象物の粘着テープへの貼着を、
粘着テープを皮膚表面に貼着、剥離して粘着面に皮膚角
質を移し取ることにより行うことを特徴とする標本の作
成方法を提供する。Further, according to the present invention, a holder having a window hole and an adhesive tape having a resin film as a base material adhered to the window hole is prepared, and an object to be observed is attached to the adhesive surface of the adhesive tape. A), apply an ultraviolet curable resin to this adhesive surface, put a cover glass on it, irradiate ultraviolet rays while pressing the cover glass and the adhesive tape with a flat surface to cure the ultraviolet curable resin. In the method for producing a specimen, characterized in that, and in each of the above methods, the observation object is skin keratin, adhesion of the observation object to an adhesive tape,
Provided is a method for preparing a specimen, which is characterized in that it is carried out by applying an adhesive tape to the surface of the skin, peeling it off, and transferring the skin keratin to the adhesive surface.
【００１２】以下、本発明を皮膚標本の作製について述
べるが、他の観察対象物にも適用されることはいうまで
もない。The present invention will be described below with reference to the preparation of a skin sample, but it goes without saying that the present invention can be applied to other objects to be observed.
【００１３】＜１＞本発明に用いる紫外線硬化性樹脂 本発明に用いる樹脂は、カバーガラス及び粘着テープに
接着でき、硬化したときに透明であれば使用することが
できるが、作業能率を高めるためには、速硬性の樹脂、
例えば紫外線照射により硬化する紫外線硬化性樹脂が好
ましい。また、樹脂が硬化する際に収縮すると、標本の
平面性が悪くなるので、収縮を防ぐために無溶剤型のも
のが適している。さらに、使用時に混合・調製する必要
のない一液性の樹脂を用いると、作業時間が短縮される
ので、標本作製を自動化する点で特に好ましい。<1> Ultraviolet curable resin used in the present invention The resin used in the present invention can be used as long as it can be adhered to a cover glass and an adhesive tape and is transparent when cured, but in order to improve work efficiency. Is a fast-curing resin,
For example, an ultraviolet curable resin that is cured by ultraviolet irradiation is preferable. Further, if the resin shrinks when it hardens, the flatness of the sample deteriorates, so a solventless type is suitable for preventing the shrinkage. Furthermore, the use of a one-part resin that does not need to be mixed / prepared at the time of use shortens the working time, and is therefore particularly preferable from the viewpoint of automating the sample preparation.
【００１４】このような樹脂として、（株）スリーボン
ド製の光硬化性樹脂３０４２Ｃが挙げられる。An example of such a resin is photocurable resin 3042C manufactured by ThreeBond Co., Ltd.
【００１５】＜２＞本発明の皮膚標本の作製方法 標本を作製しようとする皮膚表面に、樹脂フィルムを基
材とした粘着テープを貼着し剥離する。この粘着テープ
には透光性のものを使用し、標本作成後に粘着テープ側
から顕微鏡で観察、撮影する場合には透明なものを使用
する。<2> Method for Preparing Skin Specimen of the Present Invention An adhesive tape having a resin film as a base material is adhered and peeled off on the surface of the skin to be prepared. A transparent material is used for this adhesive tape, and a transparent one is used for observing and photographing with a microscope from the adhesive tape side after preparing the specimen.
【００１６】皮膚から剥離した粘着テープを、窓孔を有
するホルダーに、試料が窓孔に対応するように粘着面を
ホルダーに向けて貼着し、必要に応じて角質を染色す
る。このホルダーはそれ自身で枠体を形成し、紫外線硬
化性樹脂が広がらないようにするとともに、作業をし易
くし、さらに機械による自動化を可能とするために用い
るが、ホルダーを用いずに以下の操作を同様に行っても
平面性、観察性のよい標本を作製することができる。ま
た、予め窓孔に粘着テープの粘着面を向けて貼着したホ
ルダーを用意しておき、このホルダーごと粘着面を皮膚
にあて、裏面から粘着テープを皮膚に押し当てて、皮膚
角質を貼着させてもよい。The adhesive tape peeled from the skin is attached to a holder having a window hole with the adhesive surface facing the holder so that the sample corresponds to the window hole, and the keratin is dyed if necessary. This holder forms a frame by itself, prevents the ultraviolet curable resin from spreading, makes it easier to work, and is used to enable automation by a machine. Even if the same operation is performed, a sample having good flatness and observability can be prepared. Also, prepare a holder in which the adhesive side of the adhesive tape is attached to the window hole in advance, put the adhesive side on the skin together with this holder, and press the adhesive tape from the back side against the skin to attach skin keratin. You may let me.
【００１７】次に、このホルダーを平面上に置き、窓孔
から紫外線硬化性樹脂をホルダーの上面に適下し、前記
樹脂の上に窓孔よりもわずかに小さいカバーガラスを載
せ、このカバーガラスを上方から押圧しながら、カバー
ガラスの上から紫外線照射装置を用いて紫外線照射する
ことにより樹脂を硬化させる。Next, the holder is placed on a flat surface, an ultraviolet curable resin is appropriately applied to the upper surface of the holder through the window hole, and a cover glass slightly smaller than the window hole is placed on the resin. While pressing from above, the resin is cured by irradiating ultraviolet rays from above the cover glass using an ultraviolet irradiating device.
【００１８】紫外線の照度、照射時間は、使用する樹脂
により適宜設定する。例えば、前記（株）スリーボンド
製の光硬化性樹脂３０４２Ｃを使用する場合には、紫外
線照度１４０ｍＷ／ｃｍ2、試料との距離５０ｍｍ、照
射時間６秒程度で照射する。The illuminance of ultraviolet rays and the irradiation time are appropriately set depending on the resin used. For example, when the photocurable resin 3042C manufactured by ThreeBond Co., Ltd. is used, irradiation is performed with an ultraviolet illuminance of 140 mW / cm 2 , a distance to the sample of 50 mm, and an irradiation time of about 6 seconds.
【００１９】このようにして作成した皮膚標本を顕微鏡
で観察、撮影する際には、標本の粘着テープ側から行
う。ただし、対物レンズ表面とフォーカス面との距離が
長い対物レンズを使用する場合には、カバーガラス側か
ら観察、撮影することも可能である。When the skin sample prepared in this manner is observed and photographed with a microscope, it is performed from the adhesive tape side of the sample. However, when an objective lens having a long distance between the surface of the objective lens and the focus surface is used, it is possible to observe and photograph from the cover glass side.
【００２０】[0020]
【作用】本発明の方法では、皮膚角質を移し取った粘着
テープを硬化した樹脂で固定するため、粘着テープがた
るまないので、平面性のよい皮膚標本を作製することが
できる。また、紫外線硬化性樹脂を使用するので、樹脂
の硬化を短時間で行うことができ、作業能率を高めるこ
とができる。In the method of the present invention, since the adhesive tape from which the skin keratin has been transferred is fixed with the cured resin, the adhesive tape does not sag, so that a skin specimen having good flatness can be prepared. Further, since the ultraviolet curable resin is used, the resin can be cured in a short time and the work efficiency can be improved.
【００２１】[0021]
【実施例】以下に、本発明の実施例を図面に基づいて説
明する。皮膚角質２１を移し取った透明粘着テープ２２
を、粘着面を上にして図１、２に示したホルダー１０の
裏面に、試料が窓孔１１に対応するように貼着する。次
いで、ゲンチアナバイオレットとブリリアントグリーン
の２種類の染色剤を用いて皮膚角質２１を染色する。Embodiments of the present invention will be described below with reference to the drawings. Transparent adhesive tape 22 with skin keratin 21 removed
Is attached to the back surface of the holder 10 shown in FIGS. 1 and 2 with the adhesive surface facing upward so that the sample corresponds to the window hole 11. Next, the keratin 21 of the skin is dyed with two kinds of dyes, gentian violet and brilliant green.
【００２２】本実施例のホルダー１０は長方形形状の樹
脂板であり、正方形の窓孔１１を、取扱いに便利なよう
に一方の端部に近い部位に穿けてある。窓孔１１の大き
さは、使用するカバーガラスよりもわずかに大きく設定
する。ホルダー１０の厚さは、試料、カバーガラスの厚
さを含め、樹脂の量によって適宜設定する。The holder 10 of this embodiment is a rectangular resin plate, and a square window hole 11 is formed in a portion near one end for convenient handling. The size of the window hole 11 is set to be slightly larger than the cover glass used. The thickness of the holder 10 is appropriately set depending on the amount of resin, including the thickness of the sample and the cover glass.
【００２３】このホルダー１０を平面上に置き、窓孔１
１に、ホルダー１０の上面まで（株）スリーボンド製の
光硬化性樹脂３０４２Ｃを０．０２〜０．２ｍｌ注入
し、この上に１８ｍｍ×１８ｍｍ×２ｍｍのカバーガラ
ス３０を、樹脂２３とカバーガラスとの間に空気が入ら
ないように載せ、カバーガラス３０を上方から押圧しな
がら、株式会社オーク製作所製のＨＷＭ−６２３型紫外
線硬化用スポットＵＶ装置を用いて、カバーガラスの上
から紫外線照度１４０ｍＷ／ｃｍ2、標本との距離５０
ｍｍ、照射時間６秒で紫外線照射する。この条件で紫外
線を照射させると樹脂が硬化し、ホルダー１０、粘着テ
ープ２２、樹脂２３、カバーガラス３０が一体となって
標本を構成する。他の樹脂を用いる場合には、照射条件
は適宜設定する。The holder 10 is placed on a flat surface and the window hole 1
1, 0.02 to 0.2 ml of photocurable resin 3042C manufactured by ThreeBond Co., Ltd. is injected up to the upper surface of the holder 10, and a cover glass 30 of 18 mm × 18 mm × 2 mm is placed on the resin 23 and the cover glass. Between the cover glass and the cover glass 30 while pressing the cover glass 30 from above, using an HWM-623 type UV curing spot UV device manufactured by Oak Manufacturing Co., Ltd., an ultraviolet illuminance of 140 mW / cm 2 , distance to the specimen 50
mm and irradiation time 6 seconds. When ultraviolet rays are irradiated under this condition, the resin is cured and the holder 10, the adhesive tape 22, the resin 23, and the cover glass 30 are integrated to form a sample. When another resin is used, the irradiation conditions are set appropriately.
【００２４】このようにして作製した皮膚標本を粘着テ
ープ側から顕微鏡で観察し（倍率２００倍）、視野の中
心部にフォーカスを合わせたところ、ほぼ全視野にわた
ってフォーカスが合っていた。The skin sample thus produced was observed from the adhesive tape side with a microscope (magnification: 200), and the focus was adjusted to the center of the visual field.
【００２５】[0025]
【発明の効果】本発明により、平面性、観察性の良い標
本を作製することができる。その結果、標本の顕微鏡撮
影時のフォーカス設定が容易となる。EFFECTS OF THE INVENTION According to the present invention, it is possible to produce a specimen having good flatness and observability. As a result, it becomes easy to set the focus at the time of microscopic photography of the sample.
【００２６】さらに、標本の作製が簡便かつ短時間で行
えるため、作業効率を向上することができ、標本作製の
自動化も可能となる。Further, since the preparation of the sample can be performed easily and in a short time, the working efficiency can be improved and the sample preparation can be automated.
【図１】ホルダーの裏面斜視図FIG. 1 is a rear perspective view of a holder.
【図２】ホルダーの側面断面図FIG. 2 is a side sectional view of the holder.
１０・・・ホルダー １１・・・窓孔 ２１・・・皮膚角質 ２２・・・粘着テープ ２３・・・紫外線硬化樹脂 ２４．・・透明粘着剤 ３０・・・カバーガラス 10 ... Holder 11 ... Window hole 21 ... Skin keratin 22 ... Adhesive tape 23 ... UV curable resin 24. ..Transparent adhesive 30 ... Cover glass
Claims (4)
観察対象物を貼着し、この粘着テープの粘着面に紫外線
硬化性樹脂を塗布し、その上にカバーガラスをのせ、カ
バーガラス及び粘着テープを平坦面で挟圧しつつ紫外線
を照射して紫外線硬化性樹脂を硬化させることを特徴と
する標本の作製方法。1. An object to be observed is attached to an adhesive tape having a resin film as a base material, an ultraviolet curable resin is applied to the adhesive surface of the adhesive tape, and a cover glass is placed on the adhesive surface of the adhesive tape. A method for producing a specimen, which comprises irradiating ultraviolet rays while pressing the tape on a flat surface to cure the ultraviolet curable resin.
観察対象物を貼着するとともに、窓孔を有するホルダー
を用意し、前記観察対象物を貼着した粘着テープの粘着
面が前記窓孔に対応するように粘着テープをホルダーに
貼着し、観察対象を粘着した粘着テープの粘着面に紫外
線硬化性樹脂を塗布し、その上にカバーガラスをのせ、
カバーガラス及び粘着テープを平坦面で挟圧しつつ紫外
線を照射して紫外線硬化性樹脂を硬化させることを特徴
とする標本の作製方法。2. An adhesive tape having a resin film as a base material is adhered with an object to be observed, and a holder having a window hole is prepared, and the adhesive surface of the adhesive tape to which the object to be observed is adhered is the window hole. Adhere the adhesive tape to the holder so that it corresponds to, apply the UV curable resin to the adhesive surface of the adhesive tape that adhered the observation target, put the cover glass on it,
A method for producing a specimen, which comprises irradiating ultraviolet rays while pressing a cover glass and an adhesive tape between flat surfaces to cure the ultraviolet curable resin.
樹脂フィルムを基材とした粘着テープを貼着したホルダ
ーを用意し、この粘着テープの粘着面に観察対象物を貼
着し、この粘着面に紫外線硬化性樹脂を塗布し、その上
にカバーガラスをのせ、カバーガラス及び粘着テープを
平坦面で挟圧しつつ紫外線を照射して紫外線硬化性樹脂
を硬化させることを特徴とする標本の作製方法。3. A holder having a window hole to which an adhesive tape having a resin film as a base material is attached so as to correspond to the window hole, and an object to be observed is attached to the adhesive surface of the adhesive tape. Then, this adhesive surface is coated with an ultraviolet curable resin, a cover glass is placed on the adhesive surface, and the ultraviolet curable resin is cured by irradiating ultraviolet rays while pressing the cover glass and the adhesive tape with a flat surface. To prepare a specimen.
が皮膚角質であり、観察対象物の粘着テープへの貼着
を、粘着テープを皮膚表面に貼着、剥離して粘着面に皮
膚角質を移し取ることにより行うことを特徴とする標本
の作成方法。4. The skin according to any one of claims 1 to 3, wherein the object to be observed is keratinous skin, and the object to be observed is adhered to an adhesive tape by adhering the adhesive tape to the skin surface and peeling it off. A method for preparing a specimen, which is characterized in that it is carried out by transferring keratin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4081181A JP2997361B2 (en) | 1992-04-02 | 1992-04-02 | Specimen preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4081181A JP2997361B2 (en) | 1992-04-02 | 1992-04-02 | Specimen preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH05281109A true JPH05281109A (en) | 1993-10-29 |
| JP2997361B2 JP2997361B2 (en) | 2000-01-11 |
Family
ID=13739304
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4081181A Expired - Lifetime JP2997361B2 (en) | 1992-04-02 | 1992-04-02 | Specimen preparation method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2997361B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005345191A (en) * | 2004-06-01 | 2005-12-15 | Pola Chem Ind Inc | Non-dyeing observation method for horny layer cell |
| US7666671B2 (en) * | 2005-10-11 | 2010-02-23 | Cytyc Corporation | Methods for fixing cytological samples |
| JP2017156152A (en) * | 2016-02-29 | 2017-09-07 | リンテック株式会社 | Evidence collection method, evidence collecting sheet, and method of manufacturing evidence collecting sheet |
-
1992
- 1992-04-02 JP JP4081181A patent/JP2997361B2/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005345191A (en) * | 2004-06-01 | 2005-12-15 | Pola Chem Ind Inc | Non-dyeing observation method for horny layer cell |
| US7666671B2 (en) * | 2005-10-11 | 2010-02-23 | Cytyc Corporation | Methods for fixing cytological samples |
| JP2017156152A (en) * | 2016-02-29 | 2017-09-07 | リンテック株式会社 | Evidence collection method, evidence collecting sheet, and method of manufacturing evidence collecting sheet |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2997361B2 (en) | 2000-01-11 |
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