JPH0524317Y2 - - Google Patents
Info
- Publication number
- JPH0524317Y2 JPH0524317Y2 JP1986156759U JP15675986U JPH0524317Y2 JP H0524317 Y2 JPH0524317 Y2 JP H0524317Y2 JP 1986156759 U JP1986156759 U JP 1986156759U JP 15675986 U JP15675986 U JP 15675986U JP H0524317 Y2 JPH0524317 Y2 JP H0524317Y2
- Authority
- JP
- Japan
- Prior art keywords
- neck
- culture
- shape
- opening
- diameter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000010586 diagram Methods 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 4
- 229920003002 synthetic resin Polymers 0.000 description 3
- 239000000057 synthetic resin Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Description
【考案の詳細な説明】
〈産業上の利用分野〉
本考案は、細菌培養、細胞培養、組織培養等に
用いられる培養容器であつて、特にその開口部と
なる頸部の構造に関するものである。[Detailed description of the invention] <Industrial application field> The present invention relates to a culture vessel used for bacterial culture, cell culture, tissue culture, etc., and particularly relates to the structure of the neck, which is the opening of the culture vessel. .
〈従来技術〉
従来の培養容器でキヤツプ付き頸部を持つ形状
のものは、第４図に示したように、頸部Ｃの開口
端Ａ、および容器本体Ｄと頸部Ｃとの接続部Ｂの
開口部は、いずれも円形であつて、内径もほゞ同
じ大きさであるため、接続部Ｂの開口面積が限ら
れていた。その理由は次の通りである。即ち、開
口端Ａと接続部Ｂの両方の開口面積を大きくすれ
ば、培地液の導入、ピペツト類の挿入などの操作
には至便であるが、培養容器全体の高さが増大し
形状が不安定になつて、例えば容器の積重ねが出
来ない等の欠点があつた。一方、開口端Ａと接続
部Ｂを小さくしたり頸部Ｃを細長くすれば、積重
ね等はし易く形状も安定化するが、上記の諸操作
には全く不便で、培地の試薬散布、培地からの細
胞の吸引で、培地に死角を生じてしまうという欠
点があつた。<Prior art> As shown in FIG. 4, a conventional culture container having a neck with a cap has an open end A of the neck C and a connecting part B between the container body D and the neck C. The openings are all circular and have approximately the same inner diameter, so the opening area of the connecting portion B is limited. The reason is as follows. That is, if the opening area of both the opening end A and the connecting part B is increased, operations such as introducing the culture medium and inserting pipettes will be convenient, but the height of the entire culture container will increase and the shape will be awkward. Although it became stable, there were drawbacks such as the inability to stack containers. On the other hand, if the opening end A and the connecting part B are made smaller or the neck part C is made longer and thinner, it will be easier to stack them and the shape will be stabilized. The drawback was that a dead zone was created in the culture medium when the cells were aspirated.
このような欠点は、容器本体がガラス製の培養
容器であれ、合成樹脂製の培養容器であれ必ず発
生しており、これまで最適な形状、構造として評
価の定まつたものがないのが実情である。 Such defects always occur whether the container body is made of glass or synthetic resin, and the reality is that until now there has been no established evaluation of the optimal shape or structure. It is.
〈考案の目的〉
本考案の目的は、前述の諸欠点を完全に排除
し、培養液の導入や試料採取のためのピペツト類
の挿入などの諸操作がし易く、且つ、安定した形
状の積重ねし易い構造の培養容器を提供すること
にある。<Purpose of the invention> The purpose of the invention is to completely eliminate the above-mentioned drawbacks, to make it easy to perform various operations such as introducing a culture solution and inserting pipettes for sample collection, and to create a stack with a stable shape. The purpose of the present invention is to provide a culture container with an easy-to-use structure.
〈考案の構成〉
即ち本考案は、側部壁面に開口頸部を有する培
養容器であつて、キヤツプを嵌着する頸部Ｃの開
口端Ａの内壁形状が円形であり、容器本体Ｄと頸
部Ｃとの接続部Ｂの内壁開口部は最大径Ｇが開口
端Ａの内側直径に等しく、且つ最小径Ｆがその最
大径Ｇの20〜90％の大きさの楕円形もしくは長円
形であることを特徴とする培養容器である。<Structure of the invention> That is, the present invention is a culture container having an open neck on the side wall surface, and the inner wall shape of the opening end A of the neck C into which the cap is fitted is circular, and the container body D and the neck are connected to each other. The inner wall opening of the connecting part B with the part C has an oval or oblong shape with a maximum diameter G equal to the inner diameter of the opening end A and a minimum diameter F of 20 to 90% of the maximum diameter G. This is a culture container characterized by the following.
本考案で言う培養容器とは培養瓶、培養フラス
コ、培養ローラーボトル等である。本考案は、ガ
ラス製の培養容器とか合成樹脂製培養容器とかを
問わず適用することが出来るが、培養容器頸部の
構造がとかく精度を要求されるため、キヤツプ、
容器本体とも合成樹脂製であることが望ましい。 The culture vessels referred to in the present invention include culture bottles, culture flasks, culture roller bottles, etc. The present invention can be applied to both glass culture vessels and synthetic resin culture vessels, but since the structure of the neck of the culture vessel requires precision,
It is desirable that both the container body be made of synthetic resin.
以下、図面により本考案による培養容器につい
て詳細に説明する。 Hereinafter, the culture container according to the present invention will be explained in detail with reference to the drawings.
第１図および第２図は本考案の一実施例となる
培養容器を示す図で、第１図ａは頸部の構造を示
す外観図、第２図は頸部の構造を示す断面図、第
１図ｂ，ｃは夫々開口端および接続部の形状を示
す図である。開口部となる頸部Ｃは容器本体Ｄの
側部壁面に付設されており、キヤツプを嵌着する
ための開口端Ａの内壁は円形をなしている。 Figures 1 and 2 are diagrams showing a culture vessel as an embodiment of the present invention, where Figure 1a is an external view showing the structure of the neck, Figure 2 is a sectional view showing the structure of the neck, FIGS. 1b and 1c are diagrams showing the shapes of the open end and the connecting portion, respectively. A neck C serving as an opening is attached to the side wall surface of the container body D, and the inner wall of the opening end A into which the cap is fitted has a circular shape.
これに対して、容器本体Ｄと頸部Ｃとの接続部
Ｂにおける内壁開口部は、上下方向の寸法が左右
方向に比べて小さい形状をなしており、そのよう
な形状としては、第１図ｃのような楕円形の他、
長円形、第３図ｂのような両端が円弧状をなす長
方形などがあり、その形状は特に限定されるもの
ではないが、楕円形もしくは長円形とするのが好
ましい。第３図に示したように、接続部Ｂ，Ｅの
最大径Ｇは、頸部Ｃの開口端Ａの内側直径に等し
く、また、最小径Ｆはその最大径の20〜90％、好
ましくは30〜70％の大きさとするのがよい。接続
部の最大径に対する最小径の比率が20％を下回る
ときは、接続部における内壁開口部の最小径が小
さくなりすぎて、培地液の導入、ピペツト類の挿
入が不可能になる。一方、接続部Ｂの最小径Ｆは
一定以上に大きく出来ないため、Ｆ／Ｇの比率が
90％を越えると、開口端Ａが相対的に小さくなり
易く、ピペツト挿入時に培地に死角が生じ易いと
いう前述のような従来の培養容器の諸欠点が如実
に現れる。本考案の特徴は、開口端Ａの開口面積
を、接続部Ｂで取りうる最大断面積より更に大き
くとることにより、ピペツトの操作、範囲を広
げ、且つ容易にしている点にある。 On the other hand, the inner wall opening at the connection part B between the container body D and the neck C has a shape in which the dimension in the vertical direction is smaller than in the horizontal direction, and such a shape is as shown in FIG. In addition to oval shapes like c,
The shape may be an ellipse or a rectangle with arcuate ends as shown in FIG. 3b, and although the shape is not particularly limited, it is preferably an ellipse or an oblong shape. As shown in FIG. 3, the maximum diameter G of the connecting portions B and E is equal to the inner diameter of the open end A of the neck C, and the minimum diameter F is 20 to 90% of the maximum diameter, preferably It is best to set the size to 30-70%. When the ratio of the minimum diameter to the maximum diameter of the connection part is less than 20%, the minimum diameter of the inner wall opening at the connection part becomes too small, making it impossible to introduce a culture medium or insert a pipette. On the other hand, since the minimum diameter F of the connection part B cannot be larger than a certain value, the ratio of F/G is
If it exceeds 90%, the disadvantages of conventional culture vessels as described above become apparent, such as the opening end A tends to become relatively small and blind spots are likely to occur in the culture medium when inserting a pipette. A feature of the present invention is that by making the opening area of the open end A larger than the maximum cross-sectional area that can be taken at the connecting part B, the operation range of the pipette is expanded and facilitated.
〈考案の効果〉
本考案に従うと、ピペツトが開口部に挿入しに
くいという従来の欠点をことごとく排除出来、ピ
ペツトが前後左右に亘り広範囲に操作が出来るの
で、培養容器内に死角を生じることがなく、ま
た、試料細胞類の取り残しが少なく、さらに、培
養容器の高さを大きくする必要がないので、安定
した形状の積重ねし易い構造の培養容器を提供す
ることが出来、好適である。<Effects of the invention> According to the invention, the conventional drawback that the pipette is difficult to insert into the opening can be completely eliminated, and the pipette can be operated over a wide range of directions, front and back, left and right, so there are no blind spots inside the culture container. In addition, there are few sample cells left behind, and since there is no need to increase the height of the culture container, it is possible to provide a culture container with a stable shape and a structure that is easy to stack.
第１図および第２図は本考案の一実施例となる
培養容器を示す図で、第１図ａは頸部の構造を示
す概観図、第２図は頸部の構造を示す断面図、第
１ｂ，ｃは夫々開口端および接続部の形状を示す
図である。第３図は開口端および接続部の内壁開
口部の形状を示す図である。また、第４図ａは従
来の培養容器の頸部の構造を示す断面図で、第４
図ｂ，ｃは夫々その開口端および接続部の形状を
示す図である。
Figures 1 and 2 are diagrams showing a culture container as an embodiment of the present invention, in which Figure 1a is an overview diagram showing the structure of the neck, Figure 2 is a sectional view showing the structure of the neck, 1b and 1c are diagrams showing the shapes of the open end and the connecting portion, respectively. FIG. 3 is a diagram showing the shape of the opening end and the inner wall opening of the connecting portion. In addition, Fig. 4a is a cross-sectional view showing the structure of the neck of a conventional culture container.
Figures b and c are diagrams showing the shapes of the open end and the connecting portion, respectively.
Claims (1)
て、キヤツプを嵌着する頸部Ｃの開口端Ａの内壁
形状が円形であり、容器本体Ｄと頸部Ｃとの接続
部Ｂの内壁開口部は最大径Ｇが開口端Ａの内側直
径に等しく、且つ最小径Ｆがその最大径Ｇの20〜
90％の大きさの楕円形もしくは長円形であること
を特徴とする培養容器。 The culture container has an open neck on the side wall surface, and the inner wall shape of the open end A of the neck C into which the cap is fitted is circular, and the inner wall opening of the connection part B between the container body D and the neck C is circular. The maximum diameter G of the part is equal to the inner diameter of the open end A, and the minimum diameter F is 20 to 20% of the maximum diameter G.
A culture container characterized by being 90% oval or oblong in size.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1986156759U JPH0524317Y2 (en) | 1986-10-15 | 1986-10-15 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1986156759U JPH0524317Y2 (en) | 1986-10-15 | 1986-10-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6363400U JPS6363400U (en) | 1988-04-26 |
| JPH0524317Y2 true JPH0524317Y2 (en) | 1993-06-21 |
Family
ID=31078681
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1986156759U Expired - Lifetime JPH0524317Y2 (en) | 1986-10-15 | 1986-10-15 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0524317Y2 (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62180753A (en) * | 1986-02-03 | 1987-08-08 | コスタ−・コ−ポレ−シヨン | Flask |
-
1986
- 1986-10-15 JP JP1986156759U patent/JPH0524317Y2/ja not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62180753A (en) * | 1986-02-03 | 1987-08-08 | コスタ−・コ−ポレ−シヨン | Flask |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6363400U (en) | 1988-04-26 |
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