JPH05240867A - Automatic chemical analyzer - Google Patents

Abstract

PURPOSE:To obtain an analyzer, by which anybody can simply pick up cross- contamination data between reagents even if using conditions are changed. CONSTITUTION:An analysis requesting pattern, wherein an analyzing sequence for checking cross-contamination between reagent distributing devices 7 and 10 for a predetermined number of channels and a cell 5 is determined, is stored in a memory part and allocation of channels to respective items is designated. Thus, the cross-contamination between the reagents among all items is measured by repeating this steps.

Description

Detailed Description of the Invention

[0001]

The present invention relates to a sample (serum, urine, etc.)
Related to automatic chemical analyzers for analyzing biochemical items and immune-related items in it, especially so-called single-multi type automatic using a common reagent dispenser and reaction / photometric cell for various analytical items The present invention relates to an apparatus provided with a mechanism for measuring the influence of cross contamination between reagents of a chemical analyzer.

[0002]

2. Description of the Related Art In a conventional automatic chemical analyzer, in order to avoid the influence of cross-contamination between reagents, the analysis order of each item is determined in advance, and there is a principle or empirical influence on the cross-contamination. The frequency of such combinations is reduced, and the reagent dispenser or reaction cell has the function of storing information about combinations that are likely to be affected by cross-contamination, and analysis is performed when such combinations occur. There is a method such that you can skip once and insert a wash instead.

[0003]

As described above, in the prior art, even if there is a function of storing the combination information that is likely to be affected by the cross-contamination between the reagents, the information that meets the actual usage conditions of the user. It is difficult to set. In other words, because there is a difference in the items used by each user and the difference in the analysis method for each item and the composition depending on the reagent manufacturer, it is impossible to know all of them because the combination information is too complicated to store in advance. It's difficult.

For that purpose, it is necessary to collect data on the influence of cross-contamination between reagents under the conditions of the facility, but if the user himself / herself measures in any order of analysis, the data for all combinations will be obtained. It is difficult to think about what can be taken.

Although it is possible for a reagent dispenser, regarding the cell, the analysis item of one cycle before is taken into consideration, and further, in order to prevent the influence of cross contamination in the reagent dispenser, the second cycle is performed. It is very difficult to decide the analysis item. Even if the analysis order can be determined, it takes a great deal of effort to make an analysis request according to the order.

In view of the above problems, an object of the present invention is to provide an apparatus capable of easily collecting data on cross-contamination between reagents in any facility.

[0007]

The above-mentioned object is to store an analysis request pattern of an analysis order for checking cross-contamination in a reagent dispenser and a cell between a predetermined number of channels. This can be achieved by specifying which item to allocate to the channel.

That is, according to the present invention, a storage unit for storing an analysis request pattern in which an analysis order for checking cross-contamination in a reagent dispenser and a cell between a predetermined number of channels is stored, It is characterized by including a designating section for designating which item is allocated to which channel and a measuring section for measuring cross-contamination between reagents by repeating the designation of items.

In the present invention, when checking the cross-contamination between reagents, a fixed position for setting one sample used for checking is separately provided, and one container is used for all the analyzes. It is preferred to use the same sample in.

[0010]

By assigning the items as described above and executing the analysis, data of cross-contamination between the reagents between the items can be obtained.

Furthermore, by allocating and analyzing different combinations of items, data of cross-contamination between reagents between new items can be obtained. In this way, the data of cross-contamination between reagents of all combinations can be obtained only by repeating the same procedure while changing the allocation of items.

[0012]

Embodiments of the present invention will be described below with reference to FIG. In a normal analysis, a sample (serum, urine, etc.) 3 is prepared in a sample cup 2 on a sample rack 1, several specified items are requested for analysis for each sample, and the rack is a sampling probe. At the position of 4, the amount of sample determined for each item was aspirated,
It is dispensed into one of the reaction cells 5 arranged on the circumference.

The cell is driven by the reaction cell driving mechanism 6.
The first reagent 9 of the target item on the first reaction reagent tray 8 sucked by the first reagent dispenser 7 is added at that position by advancing by half a revolution and the reaction is started. 10 reaction cells
There are eight, and it is configured to advance by one half every 6 seconds.

When the previous cell reaches the second reagent dispensing position after a few minutes, the second reagent 12 of the target item on the second reagent tray 11 sucked by the second reagent dispenser 10 is added, and the reaction is performed. Goes further. Since the analysis of a plurality of different items is requested for each sample, the first reagent 9 and the second reagent 12 of the target items are positioned at the suction position by rotating the first reagent tray 8 and the second reagent tray 11. Therefore, the first reagent dispenser 7 and the second reagent dispenser 10 are cleaned for each item, and then aspirate reagents of different items.

Therefore, if the cleaning is not complete, cross-contamination between reagents in the reagent dispenser will occur.

While the cell is advancing, the reaction liquid in the cell is measured for the change in absorbance at the analysis wavelength of each item by a spectroscope (not shown), and accordingly the concentration (or activity value) of each item is determined. ) Is calculated. The cell whose analysis has been completed is reused by the cell cleaning unit 13. Since the cell may be used for analysis of a different item from the previous time, cross-contamination between reagents in the cell will occur if the washing is not perfect.

Even if cross-contamination between reagents in the reagent dispenser and the cell occurs, there is no problem if the analysis result is not affected, but if it is affected, an incorrect analysis result will be given. So the ones that have an impact are
In the case of the combination, the influence must be removed by adding washing instead of analysis.

In order to obtain the information, it is necessary to collect data on the presence or absence of the influence of cross-contamination between reagents according to the conditions (item and reagent maker) at each facility. Conventionally, in order to obtain such data, for cross-contamination between reagents by a reagent dispenser, after cleaning all cells cleanly, make an analysis request as shown in Table 1 and Table 2 from the keyboard, It was necessary to perform the analysis with the same sample.

[0019]

[Table 1]

[Table 2] Tables 1 and 2 show an example of measuring the effect of reagent cross-contamination among 14 items, but if the number of items changes, it is necessary to change the request pattern accordingly, and make no mistake from the keyboard. As you can see, entering a request was a difficult task.

Regarding cross-contamination between reagents in the cell, a request as shown in Tables 3 and 4 is made from the keyboard, and first, the analysis of the items in the first lap is performed, and then the other cross-contamination is performed in the second lap. It was necessary to perform an analysis of the item. The reason why each item is analyzed twice in succession is to remove the influence of cross-contamination between reagents by the reagent dispenser.

[0021]

[Table 3]

[Table 4] The examples in Tables 3 and 4 are examples in which four items of CH1 to CH4 were analyzed on the first lap and then the other 13 items were analyzed on the second lap. Similarly to this, CH5 on the first lap ... After analyzing
Repeated analysis of other items is required. In the example of this device, it is possible to have up to 30 items (30 CH), and it becomes more complicated as the number of items increases, so it seems that it is difficult for the user to deal with it in practice.

The present invention eliminates the above-mentioned complicated analysis request, absorbs the same sample from the separately provided sample container 14, and simultaneously collects the data of cross-contamination between reagents by both the reagent dispenser and the cell. The present invention provides a taking device and a program for executing the taking device is incorporated in the main CPU in FIG. An example is shown below.

Table 5 shows an analysis request pattern for observing the influence of cross contamination between reagents in the reagent dispenser and cell between item A and the other 10 items (B to K). 207 to 207 are fixedly stored in the memory of the main CPU.

[0024]

[Table 5] Thereby, as shown in Table 6, the influence of cross-contamination between the reagents in the reagent dispenser and the cell can be seen from the analysis result corresponding to each sample number.

[0025]

[Table 6] When the number of analysis items is 11, as shown in Table 7, A to CH1, B to K to CH2 to CH11 (first round) A to CH2, B to K CH3 to CH11, CH1 (second round) :::: CH11 for A, CH1 to CH10 for B to K, (11th round) and after washing each round cell, after inputting which channel item is to be allocated to A to K, the determined pattern By repeating the above analysis 11 times, it is possible to obtain data on the influence of reagent cross-contamination in the reagent dispenser and cell among all items.

[0026]

[Table 7] In the case of 11 items or more, it is only necessary to change the items to be assigned to A to K and repeat. Examples of 16 items, 21 items, and 24 items are shown in Tables 8, 9, and 10, respectively.

[0027]

[Table 8]

[Table 9]

[Table 10]

[0028]

According to the conventional method, it is necessary to input the analysis item (channel) for each sample number for several hundred samples from the keyboard every time analysis is performed, and the pattern also differs depending on the total number of items and the reagent The pattern of the cross-contamination check between reagents in the dispenser and the pattern of the cross-contamination check between reagents in the cell are also different, so it is difficult to input.

In the present invention, the item A and the other 10 items (B
~ K), a pattern for taking the data of the influence of cross-contamination between reagents is determined and stored, and depending on the total number of items, the number of times (several rounds) of the analysis is changed to AK for each time. You only have to enter which item (channel) to allocate, and the pattern is decided, so
It is easy to analyze the analyzed data.

Further, since the data of the cross-contamination between the reagents in the reagent dispenser and the data of the cross-contamination between the reagents in the cell can be obtained at the same time, when adding one new item, select the additional item as A. By selecting the conventional items from B to K, there is an advantage that the influence of cross-contamination between reagents can be easily checked.

By incorporating the above-mentioned dedicated program in the apparatus, the user can easily use it, and by providing a dedicated sample sampling position, sampling can be performed from one sample container, and the rack can be used. It is not necessary to arrange a large number of sample cups on the top, which not only saves time and labor, but also eliminates the need for a dead volume, so that the sample amount can be small, and at the same time, the variation between samples due to evaporation or the like can be eliminated.

[Brief description of drawings]

FIG. 1 is a schematic view of an apparatus of the present invention.

[Explanation of symbols]

 1: Sample Rack 2: Sample Cup 5: Reaction Cell 7: First Reagent Dispenser 8: First Reagent Tray 10: Second Reagent Dispenser 11: Second Reagent Tray 14: Sample Container

Claims (1)

[Claims] 1. A storage unit for storing an analysis request pattern in which an analysis order for checking cross-contamination in a reagent dispenser and a cell between a predetermined number of channels is stored, and which channel An automatic chemical analyzer, comprising: a designating section for designating whether or not to allocate an item and a measuring section for measuring cross-contamination between reagents by repeating the specification of the item.