JPH05201843A - Composition for oral cavity application - Google Patents

Abstract

PURPOSE:To provide a composition for oral cavity application such as candy, chewing gum, dentifrice and mouth wash effective for preventing dental caries and periodontosis. CONSTITUTION:The objective composition contains 0.01-1.0wt.%, preferably 0.05-0.5wt.% (in terms of dry solid) of an extract extracted from birch known as a crude herb drug by conventional extraction method. The extraction solvent is e.g. a low-polarity solvent such as hexane and ether, a high-polarity solvent such as acetone, ethyl acetate, ethanol and water or their mixture. The use of a low-polarity solvent such as hexane and ether is preferable from the viewpoint of antibacterial property.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an oral composition such as candy, gum, toothpaste and mouthwash which is effective in preventing dental caries and periodontal disease.

[0002]

2. Description of the Related Art In recent years, as microbiological results regarding the causes of dental caries and periodontal disease, it has been clarified that these two major diseases in the oral and dental fields are caused by oral bacteria. became. That is, glucosyltransferase (hereinafter referred to as GTase), which is an extracellular enzyme of oral microorganisms, particularly Streptococcus mutans (hereinafter referred to as S. mutans).
By this, sucrose in the food is converted into a sticky polysaccharide (glucan), and this glucan adheres to the tooth surface to form an aggregate of bacterial cells, that is, plaque. Microorganisms (lactic acid bacteria and gram-negative bacteria) propagate in the dental plaque, and the glycolytic system of these microorganisms further fermentatively produces organic acids such as lactic acid.

When the pH of the tooth surface is lowered by this organic acid, the acid causes demineralization of teeth, induces dental caries, and causes toxins of Gram-negative bacteria to cause periodontal disease. Therefore, in order to prevent the occurrence of this dental caries and periodontal disease, S. It is most effective to stop the activity of mutans. That is, S. When the activity of mutans is stopped, the production of GTase, and thus the production of sticky glucan, is blocked, and naturally plaque does not occur.

Conventionally, the S. Various fungicides and antibiotics have been used for the purpose of suppressing the activity of mutans, but they have their respective drawbacks, and the satisfactory results are not always obtained. For example, antibiotics are not suitable for long-term continuous use, and bactericides such as chlorhexidine are highly toxic and have the drawback of coloring teeth and tongue. Furthermore, since antibiotics and bactericides generally kill normal bacteria in the oral cavity, the normal bacterial flora is changed to cause abnormal growth of fungi and the like, often resulting in an unfavorable situation for the living body.

[0005]

The object of the present invention is to eliminate the above-mentioned drawbacks in the prior art, to be non-toxic,
Moreover, it is to provide an oral composition capable of preventing the occurrence of dental caries and periodontal disease without killing normal bacteria in the oral cavity.

[0006]

The present inventors have found that S. In order to obtain an active ingredient having a high antibacterial activity against mutans, as a result of a screening study on various plant active ingredients, a certain herbal crude drug extract was identified as S.
The inventors have found that they have a high antibacterial action against mutans and have reached the present invention.

That is, the oral composition of the present invention is characterized by containing a birch extract as an active ingredient. Hereinafter, the present invention will be described in detail.

The birch extract used in the present invention is an extract extracted from birch known as an herbal crude drug according to a known method, and is a liquid (as an extract, or as a diluent or concentrated extract). It may be in the form of paste, or powder.

In this case, the extraction solvent is hexane,
Examples include low polar solvents such as ether, polar solvents such as acetone, ethyl acetate, ethanol, methanol and water, and mixtures thereof, but low polar solvents such as hexane and ether are preferable from the viewpoint of antibacterial properties. The extraction method is a conventional method, that is, heating under reflux in an extraction solvent, or heating,
A method such as immersion at room temperature or under cooling is adopted. In the case of a water extract, it can be used as it is or after being appropriately diluted, but it is made into a powder or paste by concentration, freeze-drying, etc., and then formulated into a suitable oral composition. Is preferably used.

The production example of the birch extract is shown below. <Production Example 1> About 1000 ml of hexane is added to 100 g of dried birch powder, and the mixture is heated under reflux for 3 hours for extraction. After filtering the extract, the solvent was distilled off using an evaporator to obtain about 1.2 g of a birch extract.

<Production Example 2> The procedure of Production Example 1 was repeated except that methanol was used in place of hexane to obtain about 1
0.6 g of birch extract was obtained.

[0012] The content of the birch extract is usually 0.01 to 1.0% by weight, preferably 0.05 to 0.5% by weight, based on the total amount of the composition as a dry solid content. In addition,
If it is less than 0.01%, the preventive effect against caries and periodontal disease is small,
Further, even if it exceeds 1.0%, it may not be possible to expect an increase in the effect commensurate with the increase in the amount.

Other components of the composition of the present invention include
What is used for the usual composition for oral cavity can be used. For example, for candy, 50-90% sugar, 10-50% starch syrup, 0-3% acid, 0.05-1
% Gum, 10-30% gum base (vegetable resin, vinyl acetate resin, ester gum, etc.), 5%
0-85% sugar, 3-10% starch syrup, other appropriate amount of fragrance, softener, toothpaste 20-90% abrasive (calcium hydrogen phosphate, calcium carbonate, etc.), 10-40
% Wetting agent (glycerin, sorbit, etc.), 0.5-2
% Surfactant (such as lauryl sulfate salt), 0.0
5-0.2% sweetener, 0.5-1.5% flavor, 0.
2 to 1.5% thickener (carboxymethyl cellulose,
Sodium alginate, carrageenan, methyl cellulose, etc.) can be added.

To the oral composition of the present invention, in addition, a bactericide such as chlorhexidine or benzalkonium is added in order to further improve the preventive effect against dental caries or periodontal disease, or a preservative, a fragrance or a coloring agent is added. Agents and the like can be added.

The dosage form of the composition for oral cavity of the present invention may be any of liquid, solid and semi-solid preparations, specifically, dentifrice, troche, liquid or powder mouthwash, coating liquid, It can be used in the form of chewing gum.

[0016]

In order to know the antibacterial action of the birch extract used in the composition of the present invention, the following test was conducted.

<Test Example (Measurement of Antibacterial Activity)> (Test Method) 9.9 ml of Brain Heart Infusion (BHI) liquid medium was added to a test tube, and then 10 m.
As a sample of g or a control, extracts of rosemary, chamomile, and mung bean were added, dispersed, and 100 μl of a culture solution of the following strain pre-cultured beforehand was added, followed by anaerobic culture at 37 ° C. After leaving it for 24 hours, it was plated on TSA (tryptosoy agar) medium and the viable cell count was measured. Strains used in the test: 1. Streptococcus mutans
mutans) IFO-13955 2. Bacillus subtilis IFO-13
721 3. Staphylococcus
aureus) IFO-12732 4. Escherichia coli IFO-3972 5. Pseudomonas aeruginosa
uginosa) IFO-13275 The bacterial strains 1 to 5 shown above are suspended in physiological saline, and 100 μl of a stock solution having a turbidity adjusted so that the initial number of bacteria is 1 × 10 ⁶ cells / ml is used as a bacterial solution. The antibacterial activity against various strains (measurement result of viable cell count) is shown in Table 1 below.

[0018]

[Table 1]

(Evaluation result) 1. S. causing caries and periodontal disease It selectively shows antibacterial activity against mutans and has no adverse effect on other bacteria. 2. Regarding the type of solvent, those extracted using a low polar solvent have high antibacterial activity. 3. Extracts of other herbal crude drugs do not show antibacterial activity, whereas extracts of birch have remarkable antibacterial activity.

[0020]

[Examples] The oral composition of the present invention will be described below with reference to Examples. The mixing ratios (parts) in the examples are all parts by weight.

[0021]

Example 1 (Candy) The components shown in Table 2 were mixed to produce a candy composition. The birch extract used was produced in the same manner as in Production Example 1.

[0022]

[Table 2]

[0023]

Example 2 A toothpaste composition was prepared by mixing the ingredients shown in Table 3. The birch extract used was produced in the same manner as in Production Example 1.

[0024]

[Table 3]

[0025]

Example 3 (Candy) A candy composition was prepared by mixing the ingredients shown in Table 4. The birch extract used was produced in the same manner as in Production Example 1.

[0026]

[Table 4]

[0027]

Example 4 (Toothpaste) The ingredients shown in Table 5 were mixed to produce a toothpaste composition. In addition,
The birch extract used was produced in the same manner as in Production Example 1.

[0028]

[Table 5]

[0029]

Since the oral composition of the present invention contains the birch extract having the above-mentioned antibacterial activity, it can effectively prevent cavities and periodontal diseases.
It can be used as toothpaste, mouthwash, etc.

Claims (2)

[Claims] 1. An oral composition comprising a birch extract. 2. The oral composition according to claim 1, wherein the content of the birch extract is 0.01 to 1% by weight.