JPH05157745A - Inspecting body - Google Patents

Abstract

PURPOSE:To make excellent the performance of adsorption of an inspection reagent and a body to be inspected which are liquid, by making an inspection reagent reaction part contain an extender and a binder of hydrophobic resin and by making it be a coating layer of which the surface be formed to be rough. CONSTITUTION:In this inspecting body 1, an inspection reagent reaction part 3 contains an extender and a binder of hydro-phobic resin and is formed on one surface of a support 2, of a coating layer of which the surface is made to be rough. Since innumerable voids are formed in the reaction part 3 by the extender filled in the coating layer and, in addition, the surface of the part is rough, the performance of adsorbing and holding a liquid inspection reagent and a liquid body to be inspected such as blood is excellent. Besides, the reaction part 3 is formed of the coating layer containing the hydrophobic resin as the binder, and when the blood collected, for instance, is dropped onto the reaction part 3, as it is, and made to react, therefore, a hemocyte component can be removed by washing with water. Therefore, it is prevented that the hemocyte component produces an adverse effect on determination of the result of reaction, and the result of inspection can be obtained quickly and easily.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention is a test used for detecting various physiologically active substances contained in body fluids such as urine, blood and lymph, test target components such as virus, and determining their concentrations. Regarding the body

[0002]

2. Description of the Related Art When discovering, diagnosing or treating various diseases, for example, various physiological activities contained in the body fluid such as urine, blood and lymph are brought into contact with the test reagent reaction part. Detection of target components such as substances and viruses, and determination of their concentrations are performed. In such an inspection, an inspection sheet in which an inspection reagent reaction part is formed on a carrier is used, and by making the inspection reagent reaction part hydrophilic, a space between the inspection reagent reaction part and a liquid test object is used. The reaction is set to proceed smoothly.

When performing a blood test using this test sheet, first, the collected blood is centrifuged to take out plasma, and this is brought into contact with the test reagent in the test reagent reaction part of the test sheet. Has been taken. Therefore, there is a problem that the inspection takes a long time and the process is complicated.

Therefore, as a method of solving the above-mentioned problems, a reaction part is formed by adsorbing an antigen or an antibody on the surface of a hydrophobic sheet, and the collected blood is dropped as it is into the reaction part to form a blood component. React with the reaction part,
Next, a method of obtaining a reaction result in the reaction portion of the inspection sheet after removing the blood cell portion by washing with water has begun to be used. This test method does not require a step of collecting plasma from the collected blood, and since the blood cell portion does not remain on the test sheet, the blood cell portion has an adverse effect on the determination of the reaction result. There are merits such as nothing.

[0005]

By the way, in an inspection sheet in which an antigen or an antibody is adsorbed on the surface of the hydrophobic sheet to form a reaction part, the antigen or the antibody is not adsorbed efficiently on the hydrophobic surface. , An accurate test result may not be obtained. Thus, in order to obtain a reaction part that is easily adsorbed with an antigen or antibody and has a hydrophobic surface, a porous film made of a fluororesin or a porous part made of hydrophobic polyurethane is formed, and the antigen or antibody is adsorbed to the part. An inspection sheet has been proposed which is provided with a reaction part.

However, the former one using the fluororesin-based porous film has the drawbacks that the porous film itself is expensive and its processability is poor, and the latter hydrophobic polyurethane is used. It was found that non-specific reactions other than the intended ones markedly appeared in the substance forming the porous portion, depending on the type of antigen or antibody to be adsorbed to the porous portion.

On the other hand, according to the present invention, the hydrophobic and liquid test reagents and analytes have good adsorption performance, and nonspecific reaction does not occur depending on the type of antigen or antibody. An inspection body having an inspection reagent reaction part is provided.

[0008]

In the test body of the present invention, the test reagent reaction part of the test body contains an extender pigment and a binder made of a hydrophobic resin, and the surface thereof is a rough surface. It is composed of a coating layer, and the test reagent and the test object are brought into contact with each other in the coating layer to obtain a reaction result between them. Therefore, in the test body of the present invention, when the test reagent is pre-filled in the coating layer, or when the test reagent is pre-adsorbed in the coating layer, and further when the test is performed The target inspection is performed by sequentially adsorbing the inspection reagent and the inspection object on the layer.

In the inspection body of the present invention having the above structure,
The test reagent reaction part is usually formed on the surface of a support made of paper, plastic, or the like, and a support having a strength required for the test is used.

The test reagent reaction part comprises a coating layer containing an extender pigment and a binder made of a hydrophobic resin and having a rough surface, and examples of extender pigments include clay and carbonic acid. Calcium, titanium dioxide, barium sulfate and the like are used, and calcium carbonate is most preferable. Further, as the binder made of a hydrophobic resin, for example, polyester resin, acrylic resin, cellulose resin, vinyl resin, polyurethane resin, vinyl chloride / vinyl acetate copolymer resin, etc. are used.
Vinyl acetate copolymer resins are preferred.

As described above, the coating layer forming the reaction portion of the test reagent needs to have a rough surface. The degree of roughness of the surface of this coating layer depends on the combination of the mixing ratio of the extender pigment and the binder of the hydrophobic resin in the coating layer, the particle size of the extender pigment, the film forming performance of the binder of the hydrophobic resin, and the like. Adjusted, generally extender pigment 1
20 parts by weight of a hydrophobic resin binder to 100 parts by weight
It is possible to obtain a coating layer having a sufficient rough surface by using the composition in an amount of about 300 parts by weight, preferably about 20 to 100 parts by weight.

The coating layer forming the reaction portion of the test reagent is formed as a coating layer containing a coating agent containing an extender pigment, a binder made of a hydrophobic resin, and other optional components, and is usually about 1 to 100 μm. Formed to a thickness of.

[0013]

In the test body of the present invention, the test reagent reaction part of the test body comprises a coating layer containing an extender pigment and a binder made of a hydrophobic resin and having a rough surface. By bringing the test reagent and the test object into contact with each other in the test reagent reaction section including the coating layer, the reaction result of the test reagent and the test object can be obtained. That is, in the test body of the present invention, when the test reagent is previously filled in the coating layer, or when the test reagent is pre-adsorbed in the coating layer, the test object is tested. By bringing the reagent into contact with the reagent reaction part, the reaction result between the test reagent and the test object can be obtained. Further, when the inspection object is inspected, the test reagent and the inspection object are sequentially adsorbed to the coating layer forming the inspection reagent reaction part, whereby the reaction result of the inspection reagent and the inspection object is obtained.

In the test body of the present invention, however, the test reagent reaction part of the test body contains an extender pigment and a binder made of a hydrophobic resin, and the surface thereof is formed by a coating layer having a rough surface. Therefore, in the test reagent reaction part of the coating layer, innumerable voids are formed by the extender pigment contained in the coating layer, and moreover, the surface thereof is formed as a rough surface. Therefore, it exerts an excellent effect on the adsorption performance and the retention performance of a liquid test reagent applied to the test reagent reaction part or a liquid test object such as blood.

Further, in the test body of the present invention, since the test reagent reaction part of the test body is formed of the coating layer using the hydrophobic resin as the binder, for example, the collected blood is directly reacted to the reaction part. When the blood component and the test reagent reaction part are made to react with each other by dripping, the blood cell part can be removed by washing with water, and therefore the blood cell part adversely affects the determination of the reaction result in the test reagent reaction part. And the test results can be obtained quickly and easily.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The concrete structure of the inspection body of the present invention will be described below with reference to the illustrated embodiments.

Example 1 The inspection object indicated by reference numeral 1 in FIG. 1 has a basis weight of 3
It consists of a support 2 made of strip-shaped paper of 50 g / m ² , width 3 cm, and length 6 cm, and a circular test reagent reaction part 3 having a diameter of 3 mm and a thickness of 10 μ formed on one surface of the support 2. .. The test reagent reaction part 3 was prepared by kneading 100 parts by weight of calcium carbonate having a particle size of 0.2 to 50 μm, 35 parts by weight of a binder of vinyl chloride / vinyl acetate copolymer resin, and 170 parts by weight of cyclohexanone. It is composed of a coating layer formed by coating and drying by silk screen printing, and the surface thereof is made rough by the calcium carbonate contained in the coating layer.

Embodiment 2 The inspection body indicated by reference numeral 4 in FIG. 2 has a thickness of 5
A support 5 made of a strip-shaped polyester film having a size of 0 μ, a width of 3 cm, and a length of 6 cm, a circular test reagent reaction part 6 having a diameter of 3 mm and a thickness of 30 μ formed on one surface of the support 5, and the circle. 2m width surrounding the test reagent reaction part 6
m and a hydrophobic portion 7 having a thickness of 5 μ and forming an annular shape. The test reagent reaction part 6 was formed by the same process as the process of forming the test reagent reaction part 3 of the first embodiment. Further, the annular hydrophobic portion 7 having a width of 2 mm is formed by silk screen printing with an ink using a hydrophobic polyester resin as a vehicle, and is formed between the test reagent reaction portion 6 and the hydrophobic portion 7. The width of the concave portion 8 is set to 1 mm.

The hydrophobic part 7 in the product of this embodiment is for forming a recess 8 between the test reagent reaction part 6 and the hydrophobic part 7. Due to the existence of the recess 8, the test reagent reaction part 6 is present.
Liquid test reagents, test liquids, etc. are prevented from flowing away to other portions, and the function of holding liquid test reagents and test liquids uniformly in the test reagent reaction section 6 is fulfilled.
In order to effectively perform the above-mentioned function by the recess 8 between the reaction part 6 and the hydrophobic part 7, the width of the recess 8 between the reaction part 6 and the hydrophobic part 7 is about 1 to 2 mm. Is preferably formed. In addition, the hydrophobic portion 7 is the test reagent reaction portion 6
When, for example, is formed in an elliptical shape, it is needless to say that it is formed in a closed loop shape so as to surround the oval-shaped test reagent reaction portion 6, and the thickness is usually 1 to 200 μm,
It is sufficient if the hydrophobic part 7 is formed in a band having a width of about 1 to 5 mm. Since the hydrophobic portion 7 literally exhibits hydrophobicity, it can be easily formed by, for example, gravure printing or silk screen printing using printing ink having a hydrophobic resin such as polyester resin or vinyl resin as a vehicle. obtain.

EXAMPLE 3 An inspection object indicated by reference numeral 9 in FIG. 3 has a thickness of 5
The support 10 made of a strip-shaped polyester film having a width of 0 μ, a width of 3 cm, and a length of 6 cm, and the same step as the step of forming the test reagent reaction part 3 of Example 1 were used to cover one surface of the support 10. The formed coating layer 11 having a thickness of 30 μ and the diameter of the central portion of the surface of the coating layer 11 is 6 mm.
Except for the circular portion, a hydrophobic portion 12 having a thickness of 5 μ is formed by silk screen printing using an ink containing a hydrophobic polyester resin as a vehicle. The test reagent reaction part in the product of this example has a diameter of 6 mm whose surface is not covered by the hydrophobic part 12.
Is a coating layer 11A forming a circular portion, and is formed so as to be recessed with respect to the hydrophobic portion 12.

[Experiment] After adsorbing an antigen on the surface of the test reagent reaction part of each test body of Examples 1 to 3 above, and then dropping one drop of blood containing an antibody against the antigen, The blood cell portion in the blood dropped on the reaction part of the test reagent was washed off with water. After that, when a test reagent that turned blue due to the presence of the antibody contained in the blood was applied, the test reagent reaction parts of each test body turned blue and the target test result was obtained. was gotten.

[0022]

[Effect] In the inspection using the inspection reagent reaction part of the inspection object of the present invention, adsorption of a liquid inspection reagent or an inspection object,
Good retention and accurate test results.

Further, in the test body of the present invention, the test reagent reaction part in the test body is composed of a coating layer containing an extender pigment and a binder of a hydrophobic resin, and is supplied at a low cost. Excellent in practical value.

[Brief description of drawings]

FIG. 1 is a cross-sectional view schematically showing a main part of an example of an inspection body of the present invention.

FIG. 2 is a sectional view schematically showing a main part of another embodiment of the inspection body of the present invention.

FIG. 3 is a cross-sectional view schematically showing a main part of another embodiment of the inspection body of the present invention.

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 Test body 2 Support body 3 Test reagent reaction part comprising a coating layer containing an extender pigment and a binder made of a hydrophobic resin, the surface of which is roughened

Claims (1)

[Claims] 1. A test body having a test reagent reaction part, wherein the test reagent reaction part comprises a coating layer containing an extender pigment and a binder of a hydrophobic resin, the surface of which is roughened. Inspection body characterized by that.