JPH0499423A - Method for culturing rice ear - Google Patents
Method for culturing rice earInfo
- Publication number
- JPH0499423A JPH0499423A JP21641690A JP21641690A JPH0499423A JP H0499423 A JPH0499423 A JP H0499423A JP 21641690 A JP21641690 A JP 21641690A JP 21641690 A JP21641690 A JP 21641690A JP H0499423 A JPH0499423 A JP H0499423A
- Authority
- JP
- Japan
- Prior art keywords
- rice
- medium
- ripening
- culture
- ear
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 26
- 235000009566 rice Nutrition 0.000 title claims abstract description 26
- 238000012258 culturing Methods 0.000 title claims abstract 3
- 238000000034 method Methods 0.000 title claims description 8
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 25
- 230000005070 ripening Effects 0.000 claims abstract description 9
- 210000005069 ears Anatomy 0.000 claims description 10
- 239000002609 medium Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 235000001014 amino acid Nutrition 0.000 description 3
- 229940024606 amino acid Drugs 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
Abstract
Description
【発明の詳細な説明】 （産業上の利用分野） 不発明は、イネの登熟方法に関する。[Detailed description of the invention] (Industrial application field) The invention relates to a method for ripening rice.
（発明が解決しようとする課題）
イネの開花後の登熟過程には、温度、湿度及び日照など
の気象条件、土壌からの養分及び水分の吸収及び移行、
茎葉部（ソース器官）における光合成と同化産物の積荷
（ローディング。(Problem to be Solved by the Invention) The ripening process of rice after flowering involves climatic conditions such as temperature, humidity and sunlight, absorption and transfer of nutrients and moisture from the soil,
Photosynthesis and loading of assimilate products in the foliage (source organ).
ｌｏａｄｉｎｇ ） 、ソース−シンク間の同化産物の
転流、穂（シンク器官）における同化産物の降荷（アン
ローディング、 ｕｎｌｏａｄｉｎｇ ）並びにでんぷ
ん合成など多くの要因が複雑に関与している。そのため
１例えば、薬剤などのイネ登熟に対する作用を調べる場
合に、その要因並びに作用点については不明瞭な場合が
多（ある。Many factors are intricately involved, including loading of assimilates, translocation of assimilates between source and sink, unloading of assimilates in the panicle (sink organ), and starch synthesis. For this reason, for example, when investigating the effects of drugs on rice ripening, the factors and points of action are often unclear.
（発明の構成）
本発明は、出穂後のイネから穂を含む外植片（ｅｘｐｌ
ａｎｔ ）を取り出し、それを培地で培養し登熟させて
、完熟したイネ種子を得る方法である。(Structure of the Invention) The present invention provides explants containing ears from rice after heading.
ant), culture it in a medium and allow it to ripen to obtain fully ripened rice seeds.
以下に、本発明の方法を詳しく説明する。The method of the present invention will be explained in detail below.
本培養に供するイネの穂は培養の目的に応じて出穂前後
数日から完熟期に至るまでの任意の時期；こ採取するこ
とができるが、好ましくは出穂５日以降、更に好ましく
は出穂５日以降の穂を採取する。イネの穂を採取する際
には、穂を有する桿を地際部より切断し４直ちに水に挿
す。その状態で、出葉以外の葉をすべて除去し、１葉に
ついては葉鞘のみ残して葉身を切断する。ただし、目的
に応じて上葉葉身またはその一部を残すこともできる。Rice ears for main culture can be collected at any time from a few days before or after heading to full ripeness depending on the purpose of the culture, but preferably after the 5th day of heading, more preferably on the 5th day after heading. Collect subsequent ears. When collecting rice ears, cut the stem containing the ears from the ground and immediately place them in water. In this state, remove all leaves other than the emergent leaves, and cut the leaf blade of one leaf leaving only the leaf sheath. However, depending on the purpose, the upper leaf blade or part of it may be left.
その後止葉節の下方数ｃｍのところで切口が稈に対して
約３０°ぐらいの角度になるように斜めに、水中で切断
する。以下の操作は無菌室内で行うが、 ３００−５０
０１１ の容器にＮａ０Ｃ１水溶液（塩素成分として
約１％）を入れ約１０分間、出葉葉鞘が完全につかるよ
うに稈を浸漬する。この際７０％エタノールの浸漬を併
用してもよい。オートクレーブ滅菌済みの培地６０ ｗ
＋１を含む１００ ｍｌ 容三角フラスコに、上記滅
菌処理済みの穂の程を挿し、フラスコの口の所で綿栓で
稈を支えて、フラスコ内を無菌状態に維持させる。この
時、桿の切口の先端がフラスコの底に丁度届くようにす
る。Then, cut diagonally in water a few centimeters below the flag node so that the cut end is at an angle of about 30° to the culm. The following operations are performed in a sterile room.
Pour an aqueous Na0C1 solution (approximately 1% chlorine content) into a No. 011 container and soak the culm for about 10 minutes so that the leaf sheaths are completely immersed. At this time, immersion in 70% ethanol may be used in combination. Autoclaved medium 60w
Insert the sterilized panicle into a 100 ml Erlenmeyer flask containing +1, and support the culm with a cotton plug at the mouth of the flask to maintain a sterile condition inside the flask. At this time, make sure that the cut end of the rod just reaches the bottom of the flask.
培地としては［植物細胞組織培養、実際・応用・展望」
（原田宏、駒嶺穆編、１９７９、理工学社、３９０−
３９１頁）などに記載のあるものに炭素源として種々の
糖を添加するものが考えられる。例えば１−２０％蔗糖
、好ましくは３−１Ｏ％蔗糖を含むムラシゲ・スクーグ
（ＭＳ）培地などが適している。また培地中の窒素源に
は硝酸態窒素、アンモニア態窒素、各種アミノ酸または
これらの２種以上の組み合わせを使用することができる
が、無機窒素の場合は硝酸態とアンモニア態窒素の共存
が好ましく、アミノ酸の場合はアスパラギンなどが適し
ている。As a medium [Plant cell tissue culture, practice, application, and prospects]
(Hiroshi Harada, Mu Komamine eds., 1979, Rikogakusha, 390-
It is possible to add various sugars as a carbon source to those described in (page 391). For example, Murashige-Skoog (MS) medium containing 1-20% sucrose, preferably 3-10% sucrose is suitable. Nitrate nitrogen, ammonia nitrogen, various amino acids, or a combination of two or more of these can be used as the nitrogen source in the medium, but in the case of inorganic nitrogen, coexistence of nitrate nitrogen and ammonia nitrogen is preferred; In the case of amino acids, asparagine etc. are suitable.
このようにして調製されたイネの穂を自然光利用型の培
養室または数千〜敵方ルックスの人工光利用型の培養室
内で温度的２０−３２℃、好ましくは昼間（６：００−
１８：００１２８℃、夜間（１８：００−６：００１２
２℃の下で約３０日間培養する。The rice ears prepared in this way are placed in a culture room using natural light or a culture room using artificial light with a temperature of 20 to 32°C, preferably during the daytime (6:00-32°C).
18:00128℃, night (18:00-6:0012
Culture at 2°C for about 30 days.
（作用）
本発明は従来知られていなかった、イネのシンク器官の
一つである穂のｉｎ ｖｉｔｒｏ培養法であり、本方法
によればシンク活性すなわち穂への物質集積の能力を直
接に測定することができ、ソース等に関与する諸要因を
排除することが可能になる。(Function) The present invention is a previously unknown in vitro culture method for panicles, which is one of the rice sink organs. According to this method, sink activity, that is, the ability to accumulate substances in the panicle, can be directly measured. This makes it possible to eliminate various factors related to sources and the like.
（発明の効果）
本発明の方法によると、次のような実用的な効果がある
。(Effects of the Invention) The method of the present invention has the following practical effects.
■本発明は各種薬剤の継続的投与及び濃度のコントロー
ル等が容易になるため、又、シンク活性のみがイネ登熟
にどのような量ニ響を及ぼすかを調べられるため、イネ
の登Ｐ櫟構の解明に極めて重要な実験系となる。■The present invention facilitates the continuous administration and concentration control of various drugs, and also makes it possible to examine the influence of sink activity alone on rice ripening. This will be an extremely important experimental system for elucidating the structure.
■本発明によるシンク活性の測定すなわち籾の稔実の様
子の測定は、イネの登熟向上剤探索の上での有力で簡便
なスクリーニング方法となる。(2) The measurement of sink activity, that is, the measurement of the fertility of paddy according to the present invention, is a powerful and simple screening method for searching for ripening improvers for rice.
ここで「登熟向上剤」とは、増収剤の一種であり、シン
ク活性の向上によるものをいう。The term "ripening improver" as used herein is a type of yield increasing agent that improves sink activity.
■アミノ酸、ビタミン、植物ホルモンなどの天然生理活
性物室や化学合成された生理活性物質を培地中に入れ、
登熟中に穂に供与することによって、それらの物質を高
濃度に含むイネの種子の作出が可能である。このことに
よって、栄養価の高い、食味の向上した又は貯蔵性の良
い種子が作成できる。また、植物生長促進物質や病虫害
防除物質などを含む種子の作出は、翌年播種される苗の
生育に好都合となり、出芽および苗立ちを向上させ生育
促進効果をもたらす。■Introducing natural physiologically active substances such as amino acids, vitamins, and plant hormones as well as chemically synthesized physiologically active substances into the medium.
By applying these substances to the ears during ripening, it is possible to produce rice seeds that contain these substances in high concentrations. This allows the creation of seeds that are highly nutritious, have improved taste, or have good shelf life. In addition, the production of seeds containing plant growth promoting substances, pest control substances, etc. is favorable for the growth of seedlings sown the following year, improves germination and seedling establishment, and has a growth promoting effect.
■本発明によれば、圃場で栽培されたイネの上穂期以後
は、王として穂からなる外植片をガラス室や室内で栽培
することになる。このことは圃場の有効利用、病害虫に
おかされる恐れのないイネの種子の生産につながる。According to the present invention, after the ear stage of rice cultivated in the field, explants consisting of ears are cultivated in a glass chamber or indoors. This leads to effective use of the field and the production of rice seeds that are free from pests and diseases.
（実施例）
出穂５日後の穂（品種ともひかり）を採取し、上葉以外
の葉をすべて除去し、上葉については葉鞘のみ残して葉
身を切断した。止葉節の下方３ｃ１のところで水中にて
切断した後、３００＋ｎｌの容器中のＮａ０Ｃ１水溶液
（塩素成分として約１％）に約１０分間、出葉葉鞘が完
全につかるように浸漬した。その後、蔗糖５％を含む、
ムラシゲ・スクグ（ＭＳ）培地（ｐＨ５，ｓ に調整
）を用い、自然光利用型の培養室（６，００−１８００
，２８’Ｃ／１８００６：００，２２℃）で２８日間培
養した。(Example) Ears (variety: Tomohikari) 5 days after earing were collected, all leaves except the upper leaves were removed, and the leaf blade of the upper leaves was cut leaving only the leaf sheath. After cutting in water at 3c1 below the flag leaf node, it was immersed in a Na0C1 aqueous solution (about 1% as a chlorine component) in a 300+nl container for about 10 minutes so that the emerging leaf sheath was completely immersed. Then, containing 5% sucrose,
Using Murashige-Sukug (MS) medium (adjusted to pH 5, s), a culture room using natural light (6,00-1800
, 28'C/18006:00, 22°C) for 28 days.
（試験例１）
実施例に従って培養したイネからは、表１に示すように
、圃場やポットなどで栽培された完全体のイネと同等の
籾を得ることができる。(Test Example 1) As shown in Table 1, from rice cultivated according to the example, it is possible to obtain paddy equivalent to whole rice cultivated in a field or in a pot.
（試験例２）
実施例の穂培養法によって得られた種子は、表２に示す
ように正常に発芽し、その後も正常に生育する。(Test Example 2) The seeds obtained by the panicle culture method of the example germinated normally as shown in Table 2, and continued to grow normally thereafter.
（試験例３）
実施例に準じ０．０ｌ−１ｐｐｌｎのインドール酢酸１
１ＡＡ）またはジベレリン（ＧＡ）を含む培地中で穂培
養した種子を催芽させずに直播きした際の初期生育の様
子を表３に記す。表３から明らかなように、薬剤を供与
され登熟したイネの種子の初期生覆土３ ｃｍ＋の深播
きで
播種後２０日後
の呂芽した苗の生育状態
５％のＦ［を含むムラシゲ・スクーグ培地(Test Example 3) 0.0l-1ppln of indole acetic acid 1 according to the example
Table 3 shows the initial growth when seeds cultured in panicles in a medium containing 1AA) or gibberellin (GA) were sown directly without germination. As is clear from Table 3, the growth status of the seedlings that sprouted 20 days after seeding with the seedlings of rice seedlings that were seeded with the drug and ripened at a depth of 3 cm+ was as follows: Murashige Skoog containing 5% F[ Culture medium
Claims (1)
、それを培地で培養し、イネ種子を登熟させる方法。A method of removing explants including ears from rice, culturing them in a medium, and ripening the rice seeds.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21641690A JPH0499423A (en) | 1990-08-16 | 1990-08-16 | Method for culturing rice ear |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21641690A JPH0499423A (en) | 1990-08-16 | 1990-08-16 | Method for culturing rice ear |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0499423A true JPH0499423A (en) | 1992-03-31 |
Family
ID=16688222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21641690A Pending JPH0499423A (en) | 1990-08-16 | 1990-08-16 | Method for culturing rice ear |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0499423A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011195508A (en) * | 2010-03-19 | 2011-10-06 | Cosmo Oil Co Ltd | Agent for giving deep-seeding tolerance to rice |
-
1990
- 1990-08-16 JP JP21641690A patent/JPH0499423A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011195508A (en) * | 2010-03-19 | 2011-10-06 | Cosmo Oil Co Ltd | Agent for giving deep-seeding tolerance to rice |
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