JPH04896Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Technical Field of the Invention] The present invention relates to a shearle. More specifically, the present invention relates to a shear tray having light shielding means on the outer peripheral surface of the hanging side wall of the lid tray.

[Prior Art] Conventionally, new bacterial species are generally treated and cultured through the following nine steps.

It consists of (1) collecting soil, (2) diluting it with water, (3) planting it on an agar medium in a culture shear, (4) culturing it for a certain period of time in a warmer, and (5) propagating it on an agar medium. Visually identify colonies of various bacteria, (6) collect only colonies with the desired color using a platinum loop, (7) transfer them to a test tube or agar medium, and (8) culture them to see if they are promising. If it is a specific bacterium, it will be examined by repeating various tests, and (9) it will be applied to medicine, brewing, and other biotechnology fields.

With the recent rapid development of electronics technology, devices have been developed that automatically perform a series of operations as described above. For example, patent application No. 60-292496
An example of an automatic colony transplantation device is disclosed in the publication.

The automatic colony transplantation device developed as this type of automatic device selects a predetermined colony from a source culture medium in which various colonies are propagated in a mixed state, automatically selects colonies according to the selection, and The cells are collected one after another and transplanted into a medium on an object (culture) shear. Such an automatic transplanting device uses shears in units of several thousand sheets, but since there is no need for human intervention, the entire transplanting work can now be done extremely efficiently and in a short period of time.

An example of the automatic colony transplantation device as described above is shown in FIGS. 3 and 4.

In FIG. 3, an object shear conveyor line 10 and a source shear conveyor line 20 are arranged in parallel and separated by a predetermined interval. An object shear supply section (not shown) is disposed at a suitable position upstream of the object shear conveyor line 10. Similarly, a sauce shear supply section is disposed at an appropriate position upstream of the sauce shear conveyor line 20.

The shear dish 14 on the conveyor line 10 is advanced on the driving conveyor line and reaches the front position of the shear dish handling section 30a. The shear dish 14 that has reached this position is positioned by the shear dish handling section 30a. The positioning of the shear dish can be carried out, for example, by pinching the shear dish from the left and right sides with the pinch holding means 32a, as shown in FIG.

In addition to the shear dish holding means 32a, the shear dish handling section 30a is further provided with a shear dish lid attaching/detaching means 34a that may or may not be interlocked with the shear dish holding means 32a.

Sauce dishes are also handled in the same way as above.

A predetermined colony selected from the positioned and uncovered source shear 24 is positioned on the opposite side of the source shear 24 by the colony transplanting means 40, and
Transplant it to Object Shear 14 whose lid has been removed.

After the transplantation is completed, the object shear is covered again by the shear lid attaching/detaching means 34a. Thereafter, the pressing force applied to the shear pinch holding means 32a is released, the conveyor is driven, and the shear that has been transplanted is discharged from the transplanting work section. The same process is applied to the source sauce.

As shown in FIG.
0, the base end of the transplant arm 42 is fixed to the XYZ table 44. Furthermore, the transplant arm 42
A pick-up portion 48 having a colony collection and transplantation needle 46 is fixedly attached to the distal end of the tube. Transplant arm 4
2 can be moved up and down, back and forth, and left and right by the XYZ table 44.

[Problems to be Solved by the Invention] In the automatic transplanting device as described above, the presence of shears on the conveyor is optically detected for positioning and the like. When the optical detection system confirms the presence of shear, driving of the positioning mechanism etc. is started.

For example, as shown in FIG. 5, a conventional detection method includes a light emitting unit (for example, a light emitting element) 110 of an optical detection system 100 on the end surface 13 of the upper wall portion 16 of the lid plate 15 of the shear dish 14 (or 24). was emitting light. A light receiving section (for example, a light receiving element) 120 is disposed on the opposite side of the light emitting section 110 across the shear. The bottom of the tray is conveyor 10 or 20
It is not possible to place an optical detection system on the top and bottom of the shear, as they are in contact with each other.

A dish dish is a substantially perfect circular glass or synthetic resin dish having a predetermined depth. Shearle is generally transparent and allows light to pass through it well. Therefore, when light is irradiated onto the hanging side wall 17 of the lid plate of the petri dish, the light passes through the petri dish and is received by the light receiving section on the opposite side. This results in a detection result indicating that no shear exists even though there is actually a shear, which disrupts the consistent operation of the automatic transplantation device.

The upper wall of the lid plate of the shearer has a length equal to the outer diameter of the lid plate, so if the light beam from the light emitting part is accurately irradiated to the area within the thickness of the upper wall, , the light does not reach the light receiving section and the presence of shear is detected.

However, the thickness of the top wall of the lid plate is only about 1 mm at most. Therefore, for example, if the conveyor is bent by several millimeters for some reason, the light from the light emitting section 110 will pass through the upper part of the upper wall surface of the tray lid tray and enter the light receiving section. This will cause an error, similar to when irradiating a hanging side wall.
This hinders the smooth progress of the transplant work and reduces throughput.

Therefore, the purpose of the present invention is to use an optical detection system to ensure that colonies are transplanted using an automated machine.
Moreover, it is an object of the present invention to provide a shear stain that can be easily detected.

[Means for Solving the Problems] In order to solve the above-mentioned problems and also achieve the purpose of the present invention, this invention provides a lower plate and a lid plate that is attached to the lower plate. The lid plate is composed of an upper wall and a hanging side wall of a predetermined height following the upper wall, and the shear tray is provided with a light shielding means on the outer periphery or inner peripheral wall surface of the hanging side wall. provide.

[Function] The height of the hanging side wall of the chaare lid plate is approximately 1 cm to 2 cm.
It is about cm. Therefore, if a light shielding means is provided on this hanging side wall and the light beam from the light emitting part is irradiated thereon, the beam will not deviate from the area of the hanging side wall even if the conveyor conveying the shears is bent to some extent. Almost never happens.

In this way, shear detection errors are eliminated, so the colony transplantation process proceeds smoothly and throughput is improved.

[Example] Hereinafter, the shear plate of the present invention will be described in more detail with reference to the drawings.

FIG. 1 is a perspective view of one example of the shear plate of the present invention, and FIG. 2 is a perspective view of another example of the shear plate of the present invention.

As shown in FIG. 1, the shear tray of the present invention consists of a lower plate 1 and a lid plate 2, which is loosely fitted into the lower plate and has an inner diameter larger than the outer diameter of the lower plate. .

An embossed pattern 4 is provided on the outer peripheral surface of the hanging side wall 3 of the lid plate 2 as a light shielding means.
If desired, the embossed pattern can be placed on the inner peripheral surface of the depending side wall. When the light beam hits the embossed pattern, it is diffusely reflected and is effectively prevented from passing through the shear. The embossed pattern is not limited to what is illustrated. Does not transmit light and/
Alternatively, any pattern that can reflect light may be used.

Although it is sufficient to achieve the object of the present invention by providing the embossed pattern on the lid plate, it may also be provided on the outer or inner circumferential surface of the vertical side wall of the lower plate if necessary. However, since it may become difficult to close the lid tray, it is preferable not to provide an embossed pattern on the outer peripheral surface of the vertical side wall of the lower tray unless there is a particular need.

Since the shear tray is generally made of glass or synthetic resin, it is easy to provide an embossed pattern on the outer or inner circumferential surface of the hanging side wall of the lid plate.

Alternatively, as shown in FIG. 2, the object of the present invention can also be achieved by using a shear plate in which the outer peripheral surface or inner peripheral surface of the hanging side wall of the lid plate is treated with a matte finish.

In the case of a glass shear glass, if the surface is treated with hydrofluoric acid or the like, the surface can be made matte, so-called frosted glass.

In the case of synthetic resin shearling, it can be matted by buffing or other methods.

The upper wall portion 5 of the lid tray of the shear dish is preferably transparent so that the colonies on the culture medium in the shear dish can be visually recognized with the naked eye or with an imaging device.

It is sufficient to apply the matte treatment only to the outer or inner circumferential surface of the lid plate, but if particularly necessary, the outer or inner circumferential surface of the vertical side wall of the lower plate may also be subjected to the matte treatment.

Since the outer peripheral surface or the inner peripheral surface of the hanging side wall of the lid plate is subjected to a matte treatment, even if this portion is irradiated with a light beam, the light cannot pass through the shear glass. In this way, the optical detection system can reliably and easily detect shears.

[Effect of the invention] As explained above, the shear rate of the invention is approximately 1
A light shielding means is provided on the outer peripheral surface or the inner peripheral surface of the hanging side wall of the lid plate having a height of about cm to 2 cm.

In a device that automatically performs transplantation work using thousands to tens of thousands of sheets at a time, if the presence of shear sheets must be detected optically in the conveyance system, the lid tray with a light shielding mechanism must be suspended. If the side wall is irradiated with a light beam from the light emitting part, even if the conveyor conveying the shears is bent to some extent, the beam will hardly deviate out of the range of the hanging side wall.

In this way, shear detection errors are eliminated, so the colony transplantation process proceeds smoothly and throughput is improved.

[Brief explanation of the drawing]

Fig. 1 is a perspective view showing an example of the shear plate of the present invention, Fig. 2 is a perspective view of another example of the shear plate of the present invention, and Fig. 3 is a schematic plan view showing an example of the automatic colony transplantation device. , and Figure 4 shows the -
FIG. 5 is a schematic cross-sectional view along a line, and FIG. 5 is a schematic diagram of an example of detecting shear by an optical system. 1...Charle lower plate, 2...Charle lid plate, 3
... Drooping side wall, 4... Embossed pattern, 10... Object shear conveyor line, 20... Source shear conveyor line, 14... Object shear, 15... Lid plate, 16... Lid plate upper wall part , 17... hanging side wall, 24... source shearling, 30a and 30b... shearling handling section,
32a and 32b... Sheare pressure holding means,
34a and 34b...Sear tray lid attachment/detachment means, 4
0... Colony transplant means, 42... Transplant arm,
44...XYZ table, 46...Transplant needle, 48
...pickup section, 100...Shear detection optical system, 110...light emitting section, 120...light receiving section.

Claims (1)

[Claims for Utility Model Registration] (1) A tray consisting of a lower plate and a lid plate attached to the lower plate, the lid plate having an upper wall and a predetermined height extending from the upper wall. A shear tray comprising a hanging side wall, and having a light shielding means on the outer circumference or inner circumferential wall surface of the hanging side wall. (2) The shear recited in claim 1, wherein the light shielding means is a light-opaque or light-reflective stamped pattern. (3) The sheeret according to claim 1 of the utility model registration claim, wherein the light shielding means is a matte treated surface.