JPH0467916B2 - - Google Patents
Info
- Publication number
- JPH0467916B2 JPH0467916B2 JP60185612A JP18561285A JPH0467916B2 JP H0467916 B2 JPH0467916 B2 JP H0467916B2 JP 60185612 A JP60185612 A JP 60185612A JP 18561285 A JP18561285 A JP 18561285A JP H0467916 B2 JPH0467916 B2 JP H0467916B2
- Authority
- JP
- Japan
- Prior art keywords
- agent
- tracer
- sac
- liposome
- binder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000003795 chemical substances by application Substances 0.000 claims description 134
- 239000000700 radioactive tracer Substances 0.000 claims description 92
- 238000003556 assay Methods 0.000 claims description 67
- 239000012491 analyte Substances 0.000 claims description 62
- 239000011230 binding agent Substances 0.000 claims description 60
- 238000002372 labelling Methods 0.000 claims description 48
- 102000004190 Enzymes Human genes 0.000 claims description 32
- 108090000790 Enzymes Proteins 0.000 claims description 32
- 239000000126 substance Substances 0.000 claims description 28
- 239000003446 ligand Substances 0.000 claims description 27
- 239000002502 liposome Substances 0.000 claims description 27
- 230000002101 lytic effect Effects 0.000 claims description 27
- 239000002775 capsule Substances 0.000 claims description 25
- 230000002934 lysing effect Effects 0.000 claims description 16
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 14
- 229910052791 calcium Inorganic materials 0.000 claims description 14
- 239000011575 calcium Substances 0.000 claims description 14
- 239000007787 solid Substances 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- 230000000694 effects Effects 0.000 claims description 7
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 239000005515 coenzyme Substances 0.000 claims description 4
- 239000008139 complexing agent Substances 0.000 claims description 4
- 230000001419 dependent effect Effects 0.000 claims description 4
- 108010006591 Apoenzymes Proteins 0.000 claims description 3
- 230000004913 activation Effects 0.000 claims description 3
- 210000001124 body fluid Anatomy 0.000 claims description 3
- 239000010839 body fluid Substances 0.000 claims description 3
- 239000012086 standard solution Substances 0.000 claims description 3
- 239000012190 activator Substances 0.000 claims 2
- 238000000163 radioactive labelling Methods 0.000 claims 2
- 230000002285 radioactive effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 15
- 239000002609 medium Substances 0.000 description 12
- -1 alkyl phosphates Chemical class 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 7
- 230000035945 sensitivity Effects 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 6
- 239000003094 microcapsule Substances 0.000 description 6
- 239000012911 assay medium Substances 0.000 description 5
- 125000000524 functional group Chemical group 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 229910019142 PO4 Inorganic materials 0.000 description 4
- 102000015439 Phospholipases Human genes 0.000 description 4
- 108010064785 Phospholipases Proteins 0.000 description 4
- 239000000427 antigen Substances 0.000 description 4
- 102000036639 antigens Human genes 0.000 description 4
- 108091007433 antigens Proteins 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 230000008878 coupling Effects 0.000 description 4
- 238000010168 coupling process Methods 0.000 description 4
- 238000005859 coupling reaction Methods 0.000 description 4
- 230000009089 cytolysis Effects 0.000 description 4
- 229960005156 digoxin Drugs 0.000 description 4
- 239000003112 inhibitor Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 235000021317 phosphate Nutrition 0.000 description 4
- 150000003431 steroids Chemical class 0.000 description 4
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- 206010011732 Cyst Diseases 0.000 description 3
- LTMHDMANZUZIPE-AMTYYWEZSA-N Digoxin Natural products O([C@H]1[C@H](C)O[C@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@](C)([C@H](O)C4)[C@H](C4=CC(=O)OC4)CC5)CC3)CC2)C[C@@H]1O)[C@H]1O[C@H](C)[C@@H](O[C@H]2O[C@@H](C)[C@H](O)[C@@H](O)C2)[C@@H](O)C1 LTMHDMANZUZIPE-AMTYYWEZSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 208000031513 cyst Diseases 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- LTMHDMANZUZIPE-PUGKRICDSA-N digoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)[C@H](O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O LTMHDMANZUZIPE-PUGKRICDSA-N 0.000 description 3
- LTMHDMANZUZIPE-UHFFFAOYSA-N digoxine Natural products C1C(O)C(O)C(C)OC1OC1C(C)OC(OC2C(OC(OC3CC4C(C5C(C6(CCC(C6(C)C(O)C5)C=5COC(=O)C=5)O)CC4)(C)CC3)CC2O)C)CC1O LTMHDMANZUZIPE-UHFFFAOYSA-N 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- 230000002028 premature Effects 0.000 description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 2
- 102000014384 Type C Phospholipases Human genes 0.000 description 2
- 108010079194 Type C Phospholipases Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- LOUPRKONTZGTKE-LHHVKLHASA-N quinidine Chemical compound C([C@H]([C@H](C1)C=C)C2)C[N@@]1[C@H]2[C@@H](O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-LHHVKLHASA-N 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- ZFXYFBGIUFBOJW-UHFFFAOYSA-N theophylline Chemical compound O=C1N(C)C(=O)N(C)C2=C1NC=N2 ZFXYFBGIUFBOJW-UHFFFAOYSA-N 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- BQPPJGMMIYJVBR-UHFFFAOYSA-N (10S)-3c-Acetoxy-4.4.10r.13c.14t-pentamethyl-17c-((R)-1.5-dimethyl-hexen-(4)-yl)-(5tH)-Delta8-tetradecahydro-1H-cyclopenta[a]phenanthren Natural products CC12CCC(OC(C)=O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C BQPPJGMMIYJVBR-UHFFFAOYSA-N 0.000 description 1
- CHGIKSSZNBCNDW-UHFFFAOYSA-N (3beta,5alpha)-4,4-Dimethylcholesta-8,24-dien-3-ol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21 CHGIKSSZNBCNDW-UHFFFAOYSA-N 0.000 description 1
- QYIXCDOBOSTCEI-QCYZZNICSA-N (5alpha)-cholestan-3beta-ol Chemical compound C([C@@H]1CC2)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@H](C)CCCC(C)C)[C@@]2(C)CC1 QYIXCDOBOSTCEI-QCYZZNICSA-N 0.000 description 1
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical group CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 description 1
- NRJAVPSFFCBXDT-HUESYALOSA-N 1,2-distearoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCCCCCCC NRJAVPSFFCBXDT-HUESYALOSA-N 0.000 description 1
- XYTLYKGXLMKYMV-UHFFFAOYSA-N 14alpha-methylzymosterol Natural products CC12CCC(O)CC1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C XYTLYKGXLMKYMV-UHFFFAOYSA-N 0.000 description 1
- IOOMXAQUNPWDLL-UHFFFAOYSA-N 2-[6-(diethylamino)-3-(diethyliminiumyl)-3h-xanthen-9-yl]-5-sulfobenzene-1-sulfonate Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S(O)(=O)=O)C=C1S([O-])(=O)=O IOOMXAQUNPWDLL-UHFFFAOYSA-N 0.000 description 1
- POWMMMXVDBMFMP-IYGQVCONSA-N 3-[(5r,10s,12r,13s,14s,17r)-12,14-dihydroxy-10,13-dimethyl-3-oxo-2,4,5,6,7,8,9,11,12,15,16,17-dodecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2h-furan-5-one Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)C2C([C@@]4(C)CCC(=O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 POWMMMXVDBMFMP-IYGQVCONSA-N 0.000 description 1
- FPTJELQXIUUCEY-UHFFFAOYSA-N 3beta-Hydroxy-lanostan Natural products C1CC2C(C)(C)C(O)CCC2(C)C2C1C1(C)CCC(C(C)CCCC(C)C)C1(C)CC2 FPTJELQXIUUCEY-UHFFFAOYSA-N 0.000 description 1
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 1
- WDJUZGPOPHTGOT-OAXVISGBSA-N Digitoxin Natural products O([C@H]1[C@@H](C)O[C@@H](O[C@@H]2C[C@@H]3[C@@](C)([C@@H]4[C@H]([C@]5(O)[C@@](C)([C@H](C6=CC(=O)OC6)CC5)CC4)CC3)CC2)C[C@H]1O)[C@H]1O[C@@H](C)[C@H](O[C@H]2O[C@@H](C)[C@@H](O)[C@@H](O)C2)[C@@H](O)C1 WDJUZGPOPHTGOT-OAXVISGBSA-N 0.000 description 1
- ZAFNJMIOTHYJRJ-UHFFFAOYSA-N Diisopropyl ether Chemical compound CC(C)OC(C)C ZAFNJMIOTHYJRJ-UHFFFAOYSA-N 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- BKLIAINBCQPSOV-UHFFFAOYSA-N Gluanol Natural products CC(C)CC=CC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(O)C(C)(C)C4CC3 BKLIAINBCQPSOV-UHFFFAOYSA-N 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 238000012695 Interfacial polymerization Methods 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- LOPKHWOTGJIQLC-UHFFFAOYSA-N Lanosterol Natural products CC(CCC=C(C)C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 LOPKHWOTGJIQLC-UHFFFAOYSA-N 0.000 description 1
- NNJVILVZKWQKPM-UHFFFAOYSA-N Lidocaine Chemical compound CCN(CC)CC(=O)NC1=C(C)C=CC=C1C NNJVILVZKWQKPM-UHFFFAOYSA-N 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- CAHGCLMLTWQZNJ-UHFFFAOYSA-N Nerifoliol Natural products CC12CCC(O)C(C)(C)C1CCC1=C2CCC2(C)C(C(CCC=C(C)C)C)CCC21C CAHGCLMLTWQZNJ-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex⢠Substances 0.000 description 1
- 229910052771 Terbium Inorganic materials 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 125000005907 alkyl ester group Chemical group 0.000 description 1
- QYIXCDOBOSTCEI-UHFFFAOYSA-N alpha-cholestanol Natural products C1CC2CC(O)CCC2(C)C2C1C1CCC(C(C)CCCC(C)C)C1(C)CC2 QYIXCDOBOSTCEI-UHFFFAOYSA-N 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000003416 antiarrhythmic agent Substances 0.000 description 1
- 239000000924 antiasthmatic agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 229940125681 anticonvulsant agent Drugs 0.000 description 1
- 239000001961 anticonvulsive agent Substances 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 239000000480 calcium channel blocker Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 239000005081 chemiluminescent agent Substances 0.000 description 1
- LOUPRKONTZGTKE-UHFFFAOYSA-N cinchonine Natural products C1C(C(C2)C=C)CCN2C1C(O)C1=CC=NC2=CC=C(OC)C=C21 LOUPRKONTZGTKE-UHFFFAOYSA-N 0.000 description 1
- 238000005354 coacervation Methods 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 238000001212 derivatisation Methods 0.000 description 1
- RNPXCFINMKSQPQ-UHFFFAOYSA-N dicetyl hydrogen phosphate Chemical compound CCCCCCCCCCCCCCCCOP(O)(=O)OCCCCCCCCCCCCCCCC RNPXCFINMKSQPQ-UHFFFAOYSA-N 0.000 description 1
- 229940093541 dicetylphosphate Drugs 0.000 description 1
- JTXUVYOABGUBMX-UHFFFAOYSA-N didodecyl hydrogen phosphate Chemical compound CCCCCCCCCCCCOP(O)(=O)OCCCCCCCCCCCC JTXUVYOABGUBMX-UHFFFAOYSA-N 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 229960000648 digitoxin Drugs 0.000 description 1
- WDJUZGPOPHTGOT-XUDUSOBPSA-N digitoxin Chemical compound C1[C@H](O)[C@H](O)[C@@H](C)O[C@H]1O[C@@H]1[C@@H](C)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@@H]3C[C@@H]4[C@]([C@@H]5[C@H]([C@]6(CC[C@@H]([C@@]6(C)CC5)C=5COC(=O)C=5)O)CC4)(C)CC3)C[C@@H]2O)C)C[C@@H]1O WDJUZGPOPHTGOT-XUDUSOBPSA-N 0.000 description 1
- QBSJHOGDIUQWTH-UHFFFAOYSA-N dihydrolanosterol Natural products CC(C)CCCC(C)C1CCC2(C)C3=C(CCC12C)C4(C)CCC(C)(O)C(C)(C)C4CC3 QBSJHOGDIUQWTH-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011491 glass wool Substances 0.000 description 1
- RBTKNAXYKSUFRK-UHFFFAOYSA-N heliogen blue Chemical compound [Cu].[N-]1C2=C(C=CC=C3)C3=C1N=C([N-]1)C3=CC=CC=C3C1=NC([N-]1)=C(C=CC=C3)C3=C1N=C([N-]1)C3=CC=CC=C3C1=N2 RBTKNAXYKSUFRK-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000012948 isocyanate Substances 0.000 description 1
- 150000002513 isocyanates Chemical class 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- CAHGCLMLTWQZNJ-RGEKOYMOSA-N lanosterol Chemical compound C([C@]12C)C[C@@H](O)C(C)(C)[C@H]1CCC1=C2CC[C@]2(C)[C@H]([C@H](CCC=C(C)C)C)CC[C@@]21C CAHGCLMLTWQZNJ-RGEKOYMOSA-N 0.000 description 1
- 229940058690 lanosterol Drugs 0.000 description 1
- 229960004194 lidocaine Drugs 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- PWLZRLVLUJPWOB-UHFFFAOYSA-N n-ethyl-7-ethylimino-2,8-dimethylphenoxazin-3-amine Chemical compound O1C2=CC(=NCC)C(C)=CC2=NC2=C1C=C(NCC)C(C)=C2 PWLZRLVLUJPWOB-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- DDBREPKUVSBGFI-UHFFFAOYSA-N phenobarbital Chemical compound C=1C=CC=CC=1C1(CC)C(=O)NC(=O)NC1=O DDBREPKUVSBGFI-UHFFFAOYSA-N 0.000 description 1
- 229960002695 phenobarbital Drugs 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 229960001404 quinidine Drugs 0.000 description 1
- 238000007420 radioactive assay Methods 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- GZCRRIHWUXGPOV-UHFFFAOYSA-N terbium atom Chemical compound [Tb] GZCRRIHWUXGPOV-UHFFFAOYSA-N 0.000 description 1
- 229960000278 theophylline Drugs 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 229960000707 tobramycin Drugs 0.000 description 1
- NLVFBUXFDBBNBW-PBSUHMDJSA-N tobramycin Chemical compound N[C@@H]1C[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N NLVFBUXFDBBNBW-PBSUHMDJSA-N 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/829—Liposomes, e.g. encapsulation
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Description
ãçºæã®è©³çŽ°ãªèª¬æã
æ¬çºæã¯ããªã¬ã³ãçšæ€å®æ³ããã³ãã®ãããª
æ€å®æ³ã«çšãããã補åã«é¢ãããããã«è©³ãã
ã¯ãæ¬çºæã¯ãæ€å®æ床ãåäžãããªã¬ã³ãçšæ€
å®æ³ããã³ãã®ãããªæ€å®æ³ã«çšãããã補åã«
é¢ããã
æ€å®æ³ã«çšãããã補åã«é¢ãããããã«è©³ãã
ã¯ãæ¬çºæã¯ãæ€å®æ床ãåäžãããªã¬ã³ãçšæ€
å®æ³ããã³ãã®ãããªæ€å®æ³ã«çšãããã補åã«
é¢ããã
å
ç«æ€å®æ³ã¯ãäžè¬ã«ãæ€äœäžã§æž¬å®ãããã
ãéã®ç¹å®ã®è¢«æ€ç©è³ªãšæšèããã被æ€ç©è³ªãŸã
ã¯ãã®é©åœãªé¡äŒŒç©ïŒãã¬ãŒãµãŒïŒã®æ¢ç¥éãš
ãã被æ€ç©è³ªãšãã¬ãŒãµãŒã«å¯Ÿãç¹ç°çã§ããã
ã€ã³ããŒäžã®éå®ãããæ°ã®çµåå¯èœç®æã競å
ããããšã«åºã¥ããããªãã¡ãæªç¥éã®è¢«æ€ç©è³ª
ãšãæ¢ç¥éã®ãã¬ãŒãµãŒãšéå®ãããæ¢ç¥éã®ã
ã€ã³ããŒãšãå«ããã®ç³»ã«ãããŠãæ€äœäžã®è¢«æ€
ç©è³ªæ¿åºŠãé«ããªãã°ãã€ã³ããŒã«çµåãããã¬
ãŒãµãŒãæžå°ããã
ãéã®ç¹å®ã®è¢«æ€ç©è³ªãšæšèããã被æ€ç©è³ªãŸã
ã¯ãã®é©åœãªé¡äŒŒç©ïŒãã¬ãŒãµãŒïŒã®æ¢ç¥éãš
ãã被æ€ç©è³ªãšãã¬ãŒãµãŒã«å¯Ÿãç¹ç°çã§ããã
ã€ã³ããŒäžã®éå®ãããæ°ã®çµåå¯èœç®æã競å
ããããšã«åºã¥ããããªãã¡ãæªç¥éã®è¢«æ€ç©è³ª
ãšãæ¢ç¥éã®ãã¬ãŒãµãŒãšéå®ãããæ¢ç¥éã®ã
ã€ã³ããŒãšãå«ããã®ç³»ã«ãããŠãæ€äœäžã®è¢«æ€
ç©è³ªæ¿åºŠãé«ããªãã°ãã€ã³ããŒã«çµåãããã¬
ãŒãµãŒãæžå°ããã
ãã¬ãŒãµãŒãšãã€ã³ããŒã®æ¿åºŠãäžå®ã§ãã被
æ€ç©è³ªã¬ãã«ã ããå€ãããããšããã°ããã®ç³»
ã«ãããŠçµåãã¬ãŒãµãŒãšéé¢ãã¬ãŒãµãŒã®éã
枬å®ããããšã«ãã被æ€ç©è³ªã®æªç¥ã¬ãã«ã枬å®
ããæ€å®æ³ã確ç«ããããšã¯å¯èœã§ãããäžè¬ç
ãªæšèåç©ã¯ãæŸå°æ§åäœäœãè¢å ææãé µçŽ ã
ååŠçºå å€çã§ãããåºè³ªã«ãããæŸå°æ§åäœäœ
ã®æŽ»æ§ãææã®è¢å 匷床ãŸãã¯é µçŽ 掻æ§ããåæ§
ã®æ¹æ³ã§åŠçãã被æ€ç©è³ªã®æ¢ç¥éã®ããç¯å²å
ã«ããäžããããå€ãšæ¯èŒãããæšæºæ€äœã®æž¬å®
ã§åŸãããå€ããç¹å®ç³»ã«å¯Ÿããæšæºæž¬å®æ²ç·ã®
確ç«ã«äœ¿çšãã次ãã§ãã®æ²ç·ãå ¬ç¥æ€åºã«ãã
ã被æ€ç©è³ªã®æªç¥æ¿åºŠã®æž¬å®ã«çšããã
æ€ç©è³ªã¬ãã«ã ããå€ãããããšããã°ããã®ç³»
ã«ãããŠçµåãã¬ãŒãµãŒãšéé¢ãã¬ãŒãµãŒã®éã
枬å®ããããšã«ãã被æ€ç©è³ªã®æªç¥ã¬ãã«ã枬å®
ããæ€å®æ³ã確ç«ããããšã¯å¯èœã§ãããäžè¬ç
ãªæšèåç©ã¯ãæŸå°æ§åäœäœãè¢å ææãé µçŽ ã
ååŠçºå å€çã§ãããåºè³ªã«ãããæŸå°æ§åäœäœ
ã®æŽ»æ§ãææã®è¢å 匷床ãŸãã¯é µçŽ 掻æ§ããåæ§
ã®æ¹æ³ã§åŠçãã被æ€ç©è³ªã®æ¢ç¥éã®ããç¯å²å
ã«ããäžããããå€ãšæ¯èŒãããæšæºæ€äœã®æž¬å®
ã§åŸãããå€ããç¹å®ç³»ã«å¯Ÿããæšæºæž¬å®æ²ç·ã®
確ç«ã«äœ¿çšãã次ãã§ãã®æ²ç·ãå ¬ç¥æ€åºã«ãã
ã被æ€ç©è³ªã®æªç¥æ¿åºŠã®æž¬å®ã«çšããã
ãã®ãããªæ€å®æ³ã«ãããŠãäœã¬ãã«ã®è¢«æ€ç©
質ã枬å®ããããšãå¿ èŠãªå Žåãå€ãç¹ã§æ床ã¯
æãéèŠã§ããã
質ã枬å®ããããšãå¿ èŠãªå Žåãå€ãç¹ã§æ床ã¯
æãéèŠã§ããã
ããé«æ床ã®æ€å®ãæäŸããäžã€ã®è©Šã¿ã«ãã
ãŠã¯ãæ€åºå¯èœãªæšèå€ãå«æããå¢ãšçµåãã
被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ãããªããã¬ãŒ
ãµãŒã補é ãããŠããããã®ãããªæ€å®æ³ã«ãã
ãŠã¯ãå¢ã«å«ãŸãããæšèå€ã®éã¯çŽæ¥è¢«æ€ç©è³ª
ãŸãã¯ãã®é©åœãªé¡äŒŒç©ãšçµåãããæšèå€ã®é
ããå€ããããã«ãããã¬ãŒãµãŒã®åã¢ã«ã¯æšè
å€ïŒã¢ã«ä»¥äžãæããæ€å®ã®æ床ãåäžãããã
ãããªããããã¬ãŒãµãŒãæšèå€ãå«ãå¢ãã補
é ãããŠããããã®å¢ã被æ€ç©è³ªãŸãã¯ãã®é©åœ
ãªé¡äŒŒç©ã§æäœãããå Žåã«ã¯ãäžè¬ã«ãã ïŒã€
ã®å¢ã被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ã®åã¢ã«
ãšçµåããããšãã§ããã ãã§ãããšããç¹ã§ã
ãŸã ã«æ床ãéå®ãããããã®çµæããã¬ãŒãµãŒ
ãäœãã®ã«äœ¿çšãããæ€åºå¯èœãªæšèå€ã®éïŒè¢«
æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ïŒã¢ã«åœãïŒãå¢
å ããããšã«ããæ€å®ã®æ床ãããã«åäžããå¿
èŠæ§ãããã
ãŠã¯ãæ€åºå¯èœãªæšèå€ãå«æããå¢ãšçµåãã
被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ãããªããã¬ãŒ
ãµãŒã補é ãããŠããããã®ãããªæ€å®æ³ã«ãã
ãŠã¯ãå¢ã«å«ãŸãããæšèå€ã®éã¯çŽæ¥è¢«æ€ç©è³ª
ãŸãã¯ãã®é©åœãªé¡äŒŒç©ãšçµåãããæšèå€ã®é
ããå€ããããã«ãããã¬ãŒãµãŒã®åã¢ã«ã¯æšè
å€ïŒã¢ã«ä»¥äžãæããæ€å®ã®æ床ãåäžãããã
ãããªããããã¬ãŒãµãŒãæšèå€ãå«ãå¢ãã補
é ãããŠããããã®å¢ã被æ€ç©è³ªãŸãã¯ãã®é©åœ
ãªé¡äŒŒç©ã§æäœãããå Žåã«ã¯ãäžè¬ã«ãã ïŒã€
ã®å¢ã被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ã®åã¢ã«
ãšçµåããããšãã§ããã ãã§ãããšããç¹ã§ã
ãŸã ã«æ床ãéå®ãããããã®çµæããã¬ãŒãµãŒ
ãäœãã®ã«äœ¿çšãããæ€åºå¯èœãªæšèå€ã®éïŒè¢«
æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ïŒã¢ã«åœãïŒãå¢
å ããããšã«ããæ€å®ã®æ床ãããã«åäžããå¿
èŠæ§ãããã
æ¬çºæã®äžé¢ã«ããã°ãæ€å®ã«çšããããæå
ã®ïŒã€ãããã«çµåããŠæŽ»æ§ãªç¶æ ã«ããå¢æº¶è§£
å€ãæããæ€å®ã®ä»ã®æåã¯ãã®å éšã«æšèå€ãš
å¢ããæŸåºããããšãã®ã¿å¢ã溶解ããã®ã«æŽ»æ§
ãšãªãå¢æº¶è§£å€ãå«ãå¢ã§ããæ€å®æ³ãæäŸãã
ãã®ã§ããããã®æ€å®æ³ã«ãããŠã¯æŽ»æ§å¢æº¶è§£å€
ãå°ãªããšãïŒã€ã®å¢ãšæ¥è§ŠããŠå¥ã®æŽ»æ§å¢æº¶è§£
å€ãªãã³ã«æšèå€ãæŸåºãã掻æ§å¢æº¶è§£å€ã®æŸåº
ãå¥ã®å¢ã溶解ããããšã«ãªãããã®å¥ã®å¢ãã
ãã«æŽ»æ§å¢æº¶è§£å€ãšæšèå€ãæŸåºããããã«ãã
æ€å®ã«çšããããæåïŒã¢ã«ã«ã€ãæŸåºãããæš
èå€ã®éã段éçã«å¢å ããããã«ããå¢å¹ ãé
æãããã
ã®ïŒã€ãããã«çµåããŠæŽ»æ§ãªç¶æ ã«ããå¢æº¶è§£
å€ãæããæ€å®ã®ä»ã®æåã¯ãã®å éšã«æšèå€ãš
å¢ããæŸåºããããšãã®ã¿å¢ã溶解ããã®ã«æŽ»æ§
ãšãªãå¢æº¶è§£å€ãå«ãå¢ã§ããæ€å®æ³ãæäŸãã
ãã®ã§ããããã®æ€å®æ³ã«ãããŠã¯æŽ»æ§å¢æº¶è§£å€
ãå°ãªããšãïŒã€ã®å¢ãšæ¥è§ŠããŠå¥ã®æŽ»æ§å¢æº¶è§£
å€ãªãã³ã«æšèå€ãæŸåºãã掻æ§å¢æº¶è§£å€ã®æŸåº
ãå¥ã®å¢ã溶解ããããšã«ãªãããã®å¥ã®å¢ãã
ãã«æŽ»æ§å¢æº¶è§£å€ãšæšèå€ãæŸåºããããã«ãã
æ€å®ã«çšããããæåïŒã¢ã«ã«ã€ãæŸåºãããæš
èå€ã®éã段éçã«å¢å ããããã«ããå¢å¹ ãé
æãããã
ããã«è©³ããã¯ãå¢æº¶è§£å€ãçµåããŠæãããª
ã¬ã³ãïŒãã¬ãŒãµãŒïŒãšè¢«æ€ç©è³ªãå°ãªããšã被
æ€ç©è³ªã«ç¹ç°çã«çµåãããã€ã³ããŒãšæ¥è§Šãã
ããã«æ€å®ã¯å®æœãããããã¬ãŒãµãŒã®ãªã¬ã³ã
ã¯è¢«æ€ç©è³ªãšãã€ã³ããŒã®ïŒã€ãšçµåããããã€
ã³ããŒãšçµåãããã¬ãŒãµãŒã®éã¯æ€äœäžã«ãã
ã被æ€ç©è³ªã®éã«äŸåããïŒè¢«æ€ç©è³ªãšãã¬ãŒãµ
ãŒã¯ãã€ã³ããŒäžã®çµåç®æã«é¢ããŠç«¶åããã
ãŸãã¯ãã¬ãŒãµãŒã¯è¢«æ€ç©è³ªãä»ããŠãã€ã³ããŒ
ãšçµåããïŒãæ€å®ã¯ãçµåçžæïŒè¢«æ€ç©è³ªãŸã
ã¯ãã€ã³ããŒã®ããããïŒãšçµåããŠããªããã¬
ãŒãµãŒãããã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åº
å¯èœãªæšèå€ïŒåŸè¿°ããããã«ãå¢æº¶è§£å€ãšæ€åº
å¯èœãªæšèå€ã¯åãç©è³ªã®å ŽåããããïŒãå«ã
é©åœãªå¢ãšæ¥è§Šãããããã«ãå®æœãããããã®
æ¥è§Šã«ãããã¬ãŒãµãŒã«ä»ãã溶解å€ãå¢ã溶ã
ããæšèå€ãšå¢ããæŸåºããããšã掻æ§åããã
ãã«å¥ã®å¢æº¶è§£å€ãæŸåºããããæŸåºããã掻æ§
å¢æº¶è§£å€ã¯å¥ã®å¢ã溶解ããŠããã«æšèå€ãšæŽ»æ§
å¢æº¶è§£å€ãæŸåºãããããã«ããè©Šéšå¹å°äžã§æš
èå€ã®æ¿åºŠãå¢å ãããå¢ã®æº¶è§£ã«å©çšãããã
ãã¬ãŒãµãŒã®éã¯æ€äœã«ååšãã被æ€ç©è³ªã®éã«
äŸåããããäžå®æéçµéåŸã«æŸåºãããæšèå€
ã®éãŸãã¯æšèå€ãæŸåºãããå²åã¯ãæ€äœäžã®
被æ€ç©è³ªéã瀺ãã
ã¬ã³ãïŒãã¬ãŒãµãŒïŒãšè¢«æ€ç©è³ªãå°ãªããšã被
æ€ç©è³ªã«ç¹ç°çã«çµåãããã€ã³ããŒãšæ¥è§Šãã
ããã«æ€å®ã¯å®æœãããããã¬ãŒãµãŒã®ãªã¬ã³ã
ã¯è¢«æ€ç©è³ªãšãã€ã³ããŒã®ïŒã€ãšçµåããããã€
ã³ããŒãšçµåãããã¬ãŒãµãŒã®éã¯æ€äœäžã«ãã
ã被æ€ç©è³ªã®éã«äŸåããïŒè¢«æ€ç©è³ªãšãã¬ãŒãµ
ãŒã¯ãã€ã³ããŒäžã®çµåç®æã«é¢ããŠç«¶åããã
ãŸãã¯ãã¬ãŒãµãŒã¯è¢«æ€ç©è³ªãä»ããŠãã€ã³ããŒ
ãšçµåããïŒãæ€å®ã¯ãçµåçžæïŒè¢«æ€ç©è³ªãŸã
ã¯ãã€ã³ããŒã®ããããïŒãšçµåããŠããªããã¬
ãŒãµãŒãããã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åº
å¯èœãªæšèå€ïŒåŸè¿°ããããã«ãå¢æº¶è§£å€ãšæ€åº
å¯èœãªæšèå€ã¯åãç©è³ªã®å ŽåããããïŒãå«ã
é©åœãªå¢ãšæ¥è§Šãããããã«ãå®æœãããããã®
æ¥è§Šã«ãããã¬ãŒãµãŒã«ä»ãã溶解å€ãå¢ã溶ã
ããæšèå€ãšå¢ããæŸåºããããšã掻æ§åããã
ãã«å¥ã®å¢æº¶è§£å€ãæŸåºããããæŸåºããã掻æ§
å¢æº¶è§£å€ã¯å¥ã®å¢ã溶解ããŠããã«æšèå€ãšæŽ»æ§
å¢æº¶è§£å€ãæŸåºãããããã«ããè©Šéšå¹å°äžã§æš
èå€ã®æ¿åºŠãå¢å ãããå¢ã®æº¶è§£ã«å©çšãããã
ãã¬ãŒãµãŒã®éã¯æ€äœã«ååšãã被æ€ç©è³ªã®éã«
äŸåããããäžå®æéçµéåŸã«æŸåºãããæšèå€
ã®éãŸãã¯æšèå€ãæŸåºãããå²åã¯ãæ€äœäžã®
被æ€ç©è³ªéã瀺ãã
ããªãã¡ãæ¬çºæã«åŸãæ¹æ³ã«ãããåãã¬ãŒ
ãµãŒååã¯é£éç¶ãŸãã¯é段ç¶æ©æ§ã«ããå€æ°ã®
æšèå€ååãæŸåºããããšãã§ããããã«ããæ€
å®ã®å šäœçæ床ãåäžããã
ãµãŒååã¯é£éç¶ãŸãã¯é段ç¶æ©æ§ã«ããå€æ°ã®
æšèå€ååãæŸåºããããšãã§ããããã«ããæ€
å®ã®å šäœçæ床ãåäžããã
å¢æº¶è§£å€ããã³æšèå€ããã®å
éšã«å«ã¿æ€å®ã«
çšããããå¢ã¯ãå¢æº¶è§£å€ã«ãã溶解ããããåº
ãç¯å²ã®å¢ã®ãã¡ã®ïŒã€ã§ããããã®ãããªå¢ã¯
äžè¬ã«ãã®åéã§å ¬ç¥ã§ããå°èãå«ãïŒç¹ã«ãª
ããœãŒã ïŒè質å°èïŒïŒäžéå£ãŸãã¯å€éã©ã¡
ã©ïŒãããªããŒåŸ®å°ã«ãã»ã«ïŒããšãã°ã³ã¢ã»ã«
ããŒã·ãšã³ãŸãã¯çé¢éåã«ããäœããããã®ïŒ
çã§ãããæãããªãšãããæ€å®ã§çšããããå¢
ã¯ããã¬ãŒãµãŒãšããŠäœ¿ããªã¬ã³ãã«æ¥ç¶ããå¢
溶解å€ãªãã³ã«å¢ã«å«ãŸããå¢æº¶è§£å€ãšã®é¢ä¿ã
調æŽããŠãå¢ãå¢æº¶è§£å€ãšæ¥è§Šãããšã溶解ãŸã
ã¯ç Žå£ãããããã«ãããåŸè¿°ããããã«ãå¢ã«
å«ãŸããå¢æº¶è§£å€ã¯äžæŽ»æ§åœ¢ã§ãããããã«ãã
å¢ãæ©æã«ç Žå£ãããŸãã¯æº¶è§£ãããã®ã劚ãã
ãšãã§ãããå¢ã«å«ãŸããå¢æº¶è§£å€ã¯å¢ããæŸåº
ããããšæŽ»æ§ã«ãªããããã«ããå¥ã®å¢ã溶解ã
ãããšãã§ããã
çšããããå¢ã¯ãå¢æº¶è§£å€ã«ãã溶解ããããåº
ãç¯å²ã®å¢ã®ãã¡ã®ïŒã€ã§ããããã®ãããªå¢ã¯
äžè¬ã«ãã®åéã§å ¬ç¥ã§ããå°èãå«ãïŒç¹ã«ãª
ããœãŒã ïŒè質å°èïŒïŒäžéå£ãŸãã¯å€éã©ã¡
ã©ïŒãããªããŒåŸ®å°ã«ãã»ã«ïŒããšãã°ã³ã¢ã»ã«
ããŒã·ãšã³ãŸãã¯çé¢éåã«ããäœããããã®ïŒ
çã§ãããæãããªãšãããæ€å®ã§çšããããå¢
ã¯ããã¬ãŒãµãŒãšããŠäœ¿ããªã¬ã³ãã«æ¥ç¶ããå¢
溶解å€ãªãã³ã«å¢ã«å«ãŸããå¢æº¶è§£å€ãšã®é¢ä¿ã
調æŽããŠãå¢ãå¢æº¶è§£å€ãšæ¥è§Šãããšã溶解ãŸã
ã¯ç Žå£ãããããã«ãããåŸè¿°ããããã«ãå¢ã«
å«ãŸããå¢æº¶è§£å€ã¯äžæŽ»æ§åœ¢ã§ãããããã«ãã
å¢ãæ©æã«ç Žå£ãããŸãã¯æº¶è§£ãããã®ã劚ãã
ãšãã§ãããå¢ã«å«ãŸããå¢æº¶è§£å€ã¯å¢ããæŸåº
ããããšæŽ»æ§ã«ãªããããã«ããå¥ã®å¢ã溶解ã
ãããšãã§ããã
ããªããŒåŸ®å°ã«ãã»ã«ã¯åœè©²åéã§å
¬ç¥ã®æ¹æ³
ã«ãã補é ããããããã ã埮å°ã«ãã»ã«ã圢æ
ããã溶液ã«ã¯æšèå€ãšå¢æº¶è§£å€ãå«ãŸããŠã
ããããã«ããããªããŒåŸ®å°ã«ãã»ã«ã®å éšã«æš
èå€ãšæº¶è§£å€ãå«ãŸããããã«ããããã®ãããª
埮å°ã«ãã»ã«ã®èª¿è£œã¯ãããšãã°âãã¯ããšã³ã«
ãã¹ã¬ãŒã·ãšã³ã»ããã»ã·ãŒãºã»ã¢ã³ãã»ã¢ããª
ã±ãŒã·ãšã³ïŒMicroencapsulation Processes
and ApplicationïŒâããžã€ã³ã»ã€ãŒã»ãã³ããã¬
ãŒïŒJan E.VandeggerïŒèããã¬ãã ã»ãã¬ã¹
ïŒPlenum PressïŒ1974ã«èšèŒãããŠããã
ã«ãã補é ããããããã ã埮å°ã«ãã»ã«ã圢æ
ããã溶液ã«ã¯æšèå€ãšå¢æº¶è§£å€ãå«ãŸããŠã
ããããã«ããããªããŒåŸ®å°ã«ãã»ã«ã®å éšã«æš
èå€ãšæº¶è§£å€ãå«ãŸããããã«ããããã®ãããª
埮å°ã«ãã»ã«ã®èª¿è£œã¯ãããšãã°âãã¯ããšã³ã«
ãã¹ã¬ãŒã·ãšã³ã»ããã»ã·ãŒãºã»ã¢ã³ãã»ã¢ããª
ã±ãŒã·ãšã³ïŒMicroencapsulation Processes
and ApplicationïŒâããžã€ã³ã»ã€ãŒã»ãã³ããã¬
ãŒïŒJan E.VandeggerïŒèããã¬ãã ã»ãã¬ã¹
ïŒPlenum PressïŒ1974ã«èšèŒãããŠããã
åœè©²åéã§å
¬ç¥ã®ããã«ããªããœãŒã ã¯åºãç¯
å²ã®è質ãã調補ãããããšãã°ãªã³è質ãç³è
質ãã¹ããã€ããæ¯èŒçé·éã®ã¢ã«ãã«ãšã¹ã
ã«ïŒããšãã°ã¢ã«ãã«ãã¹ããšãŒããèèªé žãšã¹
ãã«ãããšãã°ã¬ã·ãã³ãèèªã¢ãã³çãå«ãã
èèªæ§ç©è³ªã®æ··åç©ããšãã°äžæ§ã¹ããã€ãã垯
é»äž¡èŠªåªæ§ç©è³ªããã³ãªã³è質ã®çµåããããŸã
䜿çšã§ãããããªã³è質ã®ä»£è¡šçäŸãšããŠã¯ãã¹
ãã€ã³ãŽããšãªã³ããžãã«ããã€ã«ãã¬ã·ãã³ç
ã§ããã代衚çã¹ããã€ããšããŠã¯ã³ã¬ã¹ãããŒ
ã«ãã³ã¬ã¹ã¿ããŒã«ãã©ãã¹ãããŒã«çã§ããã
垯é»äž¡èŠªåªæ§ååç©ã®ä»£è¡šçãªãã®ãšããŠã¯ãäž
è¬ã«ççŽ ååæ°12ãªãã30ãæãããã®ã§ããã
ã¢ããŸãã¯ãžã¢ã«ãã«ãã¹ããšãŒããšã¹ãã«ããš
ãã°ãžã»ãã«ãã¹ããšãŒãããžã©ãŠãªã«ãã¹ããš
ãŒãããžãªã¯ãã«ãã·ã«ã¹ã«ããªããŒãçã§ã
ãã
å²ã®è質ãã調補ãããããšãã°ãªã³è質ãç³è
質ãã¹ããã€ããæ¯èŒçé·éã®ã¢ã«ãã«ãšã¹ã
ã«ïŒããšãã°ã¢ã«ãã«ãã¹ããšãŒããèèªé žãšã¹
ãã«ãããšãã°ã¬ã·ãã³ãèèªã¢ãã³çãå«ãã
èèªæ§ç©è³ªã®æ··åç©ããšãã°äžæ§ã¹ããã€ãã垯
é»äž¡èŠªåªæ§ç©è³ªããã³ãªã³è質ã®çµåããããŸã
䜿çšã§ãããããªã³è質ã®ä»£è¡šçäŸãšããŠã¯ãã¹
ãã€ã³ãŽããšãªã³ããžãã«ããã€ã«ãã¬ã·ãã³ç
ã§ããã代衚çã¹ããã€ããšããŠã¯ã³ã¬ã¹ãããŒ
ã«ãã³ã¬ã¹ã¿ããŒã«ãã©ãã¹ãããŒã«çã§ããã
垯é»äž¡èŠªåªæ§ååç©ã®ä»£è¡šçãªãã®ãšããŠã¯ãäž
è¬ã«ççŽ ååæ°12ãªãã30ãæãããã®ã§ããã
ã¢ããŸãã¯ãžã¢ã«ãã«ãã¹ããšãŒããšã¹ãã«ããš
ãã°ãžã»ãã«ãã¹ããšãŒãããžã©ãŠãªã«ãã¹ããš
ãŒãããžãªã¯ãã«ãã·ã«ã¹ã«ããªããŒãçã§ã
ãã
ãªããœãŒã å¢ã¯æšèå€ããã³äžæŽ»æ§åœ¢ã®å¢æº¶è§£
å€ãå«ã氎溶液äžã§èª¿è£œãããããã«ããå¢ã¯ã
ã®å éšã«æšèå€ããã³äžæŽ»æ§å¢æº¶è§£å€ãå«ãããš
ã«ãªãããªããœãŒã å¢ã¯ã溶液äžã§æ¿ããæ¹æ
ããç¶ããŠå¢ã®å€åŽããæšèå€ãšäžæŽ»æ§å¢æº¶è§£å€
ãšãé€å»ããããšã«ãã容æã«èª¿è£œããããä»ã®
調補æ¹æ³ããŸã䜿çšããããã
å€ãå«ã氎溶液äžã§èª¿è£œãããããã«ããå¢ã¯ã
ã®å éšã«æšèå€ããã³äžæŽ»æ§å¢æº¶è§£å€ãå«ãããš
ã«ãªãããªããœãŒã å¢ã¯ã溶液äžã§æ¿ããæ¹æ
ããç¶ããŠå¢ã®å€åŽããæšèå€ãšäžæŽ»æ§å¢æº¶è§£å€
ãšãé€å»ããããšã«ãã容æã«èª¿è£œããããä»ã®
調補æ¹æ³ããŸã䜿çšããããã
å¢ã®èª¿è£œã«é¢ããããã«è©³çŽ°ã¯ãç±³åœç¹èš±ç¬¬
4342826å·ããã³PCTåœéå ¬é第W080ïŒ01515å·
ã«ãèšèŒãããŠãããâãªããœãŒã ãºïŒããã ã»
ãã€ãžã«ã«ã»ã¹ãã©ã¯ããŠã¢ã»ããŠã»ã»ã©ããŠãŒ
ãã€ã¯ã»ã¢ããªã±ãŒã·ãšã³ãºïŒLiposomesïŒ
From Physical Structure to Therapeutic
ApplicationsïŒâãã·ãŒã»ãžãŒã»ãã€ãïŒC.G.
KnightïŒèããšã«ã·ãŒããŒïŒElsevierïŒã1981ã
ãã³âã¡ã³ãã¬ã€ã³ã»ãã¡ãã€ã¯ã»ã±ãã¹ããªã€
ïŒMembrane Mimetic ChemistryïŒâããžã€ã
ã¹ã»ãšã€ãã»ããšã³ãã©ãŒïŒJanos H.FendlerïŒã
ãžãšã³ã»ãŠã€ãªãŒã»ã¢ã³ãã»ãµã³ãºïŒJohm
Wiley and SonsïŒã1982ããŸãããã«åèæç®
ãšããŠæããŠããã
4342826å·ããã³PCTåœéå ¬é第W080ïŒ01515å·
ã«ãèšèŒãããŠãããâãªããœãŒã ãºïŒããã ã»
ãã€ãžã«ã«ã»ã¹ãã©ã¯ããŠã¢ã»ããŠã»ã»ã©ããŠãŒ
ãã€ã¯ã»ã¢ããªã±ãŒã·ãšã³ãºïŒLiposomesïŒ
From Physical Structure to Therapeutic
ApplicationsïŒâãã·ãŒã»ãžãŒã»ãã€ãïŒC.G.
KnightïŒèããšã«ã·ãŒããŒïŒElsevierïŒã1981ã
ãã³âã¡ã³ãã¬ã€ã³ã»ãã¡ãã€ã¯ã»ã±ãã¹ããªã€
ïŒMembrane Mimetic ChemistryïŒâããžã€ã
ã¹ã»ãšã€ãã»ããšã³ãã©ãŒïŒJanos H.FendlerïŒã
ãžãšã³ã»ãŠã€ãªãŒã»ã¢ã³ãã»ãµã³ãºïŒJohm
Wiley and SonsïŒã1982ããŸãããã«åèæç®
ãšããŠæããŠããã
äžè¿°ããããã«ãå¢ã®å
éšã«å«ãŸããå¢æº¶è§£å€
ã¯ãå¢ãæ©ãææã«ç Žå£ãããã®ã劚ãããã«äž
掻æ§åœ¢ã§ãªããŠã¯ãªããããŸãå¢æº¶è§£å€ã¯å¢ãã
æŸåºããããšãã¯æŽ»æ§åœ¢ã§ãªããŠã¯ãªããªããã
ãªãã¡ãããšãã°å¢æº¶è§£å€ãšããŠé µçŽ ã䜿çšãã
å Žåãå¿ èŠäžå¯æ¬ ãªéå±ã€ãªã³ããšãã°ã«ã«ã·ãŠ
ã ã®äžååšäžã«å¢ã®å éšã«é µçŽ ãå°å ¥ããããšã«
ããé µçŽ æº¶è§£å€ãå¢å éšã«äžæŽ»æ§ãªåœ¢ã§ä¿æã
ããããã¯ããšãã°ãšãã¬ã³ãžã¢ãã³åé ¢é ž
ïŒEDTAïŒã®ãããªã«ã«ã·ãŠã é¯åå€ã䜿çšãã
ããšã«ããè¡ãªãããã«ã«ã·ãŠã ã®äžååšäžã§ã¯
é µçŽ æº¶è§£å€ã¯å¢ã溶解ããªããäžæŽ»æ§åœ¢ã®é µçŽ 溶
解å€ïŒã«ã«ã·ãŠã ã®äžååšïŒãã«ã«ã·ãŠã ã«å¯ã
ã 溶液ãžæŸåºãããšæº¶è§£å€ã掻æ§åãããŠä»ã®å¢
ã溶解ããããšãã§ããã
ã¯ãå¢ãæ©ãææã«ç Žå£ãããã®ã劚ãããã«äž
掻æ§åœ¢ã§ãªããŠã¯ãªããããŸãå¢æº¶è§£å€ã¯å¢ãã
æŸåºããããšãã¯æŽ»æ§åœ¢ã§ãªããŠã¯ãªããªããã
ãªãã¡ãããšãã°å¢æº¶è§£å€ãšããŠé µçŽ ã䜿çšãã
å Žåãå¿ èŠäžå¯æ¬ ãªéå±ã€ãªã³ããšãã°ã«ã«ã·ãŠ
ã ã®äžååšäžã«å¢ã®å éšã«é µçŽ ãå°å ¥ããããšã«
ããé µçŽ æº¶è§£å€ãå¢å éšã«äžæŽ»æ§ãªåœ¢ã§ä¿æã
ããããã¯ããšãã°ãšãã¬ã³ãžã¢ãã³åé ¢é ž
ïŒEDTAïŒã®ãããªã«ã«ã·ãŠã é¯åå€ã䜿çšãã
ããšã«ããè¡ãªãããã«ã«ã·ãŠã ã®äžååšäžã§ã¯
é µçŽ æº¶è§£å€ã¯å¢ã溶解ããªããäžæŽ»æ§åœ¢ã®é µçŽ 溶
解å€ïŒã«ã«ã·ãŠã ã®äžååšïŒãã«ã«ã·ãŠã ã«å¯ã
ã 溶液ãžæŸåºãããšæº¶è§£å€ã掻æ§åãããŠä»ã®å¢
ã溶解ããããšãã§ããã
å¢æº¶è§£å€ã¯æ€å®ã«çšããããå¢ã溶解ãããåº
ãç¯å²ã®ææã®ãã¡ã®ïŒã€ã§ããã䜿çšãããç¹
å®ã®å¢æº¶è§£å€ã¯æ€åºã«çšããããå¢ã«ããã奜ãŸ
ãã溶解å€ã¯é µçŽ ã§ãããç°ãªã€ãå¢ã溶解ãã
ãé µçŽ ãåœæ¥è ã«å ¬ç¥ã§ãããããªãã¡ãããšã
ã°ãã¹ããªããŒãŒããã³ã¢ã«ã«ãªãã¹ããšãŒãã¯
é µçŽ æº¶è§£å€ã«é©ãããããã¯ããšãã°EDTAã®
ãããªã«ã«ã·ãŠã é¯åå€ãçšããããšã«ããäžæŽ»
åãããããããã¢ãŒãŒé µçŽ ããŸããŒã©ãã³åŸ®å°
ã«ãã»ã«ã溶解ããã®ã«å¹æçã§ããããšãç¥ã
ããŠããã
ãç¯å²ã®ææã®ãã¡ã®ïŒã€ã§ããã䜿çšãããç¹
å®ã®å¢æº¶è§£å€ã¯æ€åºã«çšããããå¢ã«ããã奜ãŸ
ãã溶解å€ã¯é µçŽ ã§ãããç°ãªã€ãå¢ã溶解ãã
ãé µçŽ ãåœæ¥è ã«å ¬ç¥ã§ãããããªãã¡ãããšã
ã°ãã¹ããªããŒãŒããã³ã¢ã«ã«ãªãã¹ããšãŒãã¯
é µçŽ æº¶è§£å€ã«é©ãããããã¯ããšãã°EDTAã®
ãããªã«ã«ã·ãŠã é¯åå€ãçšããããšã«ããäžæŽ»
åãããããããã¢ãŒãŒé µçŽ ããŸããŒã©ãã³åŸ®å°
ã«ãã»ã«ã溶解ããã®ã«å¹æçã§ããããšãç¥ã
ããŠããã
äžè¿°ããããã«ãå¢ã«å«ãŸãã溶解å€ã¯äžæŽ»æ§
圢ãšãæ©æã«å¢ã溶解ãŸãã¯ç Žå£ããããšãé²ã
ãªããã°ãªããªãã奜ãŸããå®æœæ æ§ã«ããã°ã
é»å®³å€ãžãå¢å éšã§æº¶è§£é µçŽ ã®é µçŽ 掻æ§ãé»å®³ã
ãã€æ€å®å¹å°ã§åžéããããšãã«ã¯æº¶è§£é µçŽ ã®æº¶
解äœçšãé»æ¢ããªãæ¿åºŠã§å¢å éšã«å«ãŸããŠã
ããäžè¿°ããããã«ãEDTAãã«ã«ã·ãŠã äŸå
æ§é µçŽ ãäžæŽ»åããã®ã«çšããããããEDTA
ã®ä»£è¡šçæ¿åºŠïŒã«ã«ã·ãŠã äŸåæ§é µçŽ ãé»å®³ã
ãïŒã¯ïŒã10ïœïŒã§ããããªããœãŒã å ãžã®é»å®³
å€ã®ãã©ãã容éã¯æ€å®ç³»ã®å šå®¹éã«æ¯èŒããŠé
åžžã«å°ãªãããã®ãã溶解æã«é»å®³å€ã¯éåžžã«åž
éãããããšã«ãªãïŒåžéçã¯ïŒïŒ1000ãïŒïŒ
100000ãŸãã¯ãã以äžã®ç¯å²ã§ããïŒãé»å®³å€ã¯
氎溶æ§ã§ãããé µçŽ åã³æšèå€ãšåæ§ã®æ¹æ³ã§å
æã«å°å ¥ããããäžæŽ»æ§åã®å¥ã®æ¹æ³ã¯ãå ¬ç¥æ
è¡ã«ããããããè£é µçŽ ãé€å»ããããšã«ããã
ãã®ããã«ããŠåŸãããã¢ãé µçŽ ãå°å ¥ããè£é µ
çŽ ããªããœãŒã å€éšã®æº¶æ¶²ã«ä¿æããã溶解æã
äžæŽ»æ§åœ¢ã®ã¢ãé µçŽ ã§ããå°å ¥ãããé µçŽ ãè£é µ
çŽ ãšäžç·ã«ãªã€ãŠæŽ»æ§é µçŽ ã圢æããã
圢ãšãæ©æã«å¢ã溶解ãŸãã¯ç Žå£ããããšãé²ã
ãªããã°ãªããªãã奜ãŸããå®æœæ æ§ã«ããã°ã
é»å®³å€ãžãå¢å éšã§æº¶è§£é µçŽ ã®é µçŽ 掻æ§ãé»å®³ã
ãã€æ€å®å¹å°ã§åžéããããšãã«ã¯æº¶è§£é µçŽ ã®æº¶
解äœçšãé»æ¢ããªãæ¿åºŠã§å¢å éšã«å«ãŸããŠã
ããäžè¿°ããããã«ãEDTAãã«ã«ã·ãŠã äŸå
æ§é µçŽ ãäžæŽ»åããã®ã«çšããããããEDTA
ã®ä»£è¡šçæ¿åºŠïŒã«ã«ã·ãŠã äŸåæ§é µçŽ ãé»å®³ã
ãïŒã¯ïŒã10ïœïŒã§ããããªããœãŒã å ãžã®é»å®³
å€ã®ãã©ãã容éã¯æ€å®ç³»ã®å šå®¹éã«æ¯èŒããŠé
åžžã«å°ãªãããã®ãã溶解æã«é»å®³å€ã¯éåžžã«åž
éãããããšã«ãªãïŒåžéçã¯ïŒïŒ1000ãïŒïŒ
100000ãŸãã¯ãã以äžã®ç¯å²ã§ããïŒãé»å®³å€ã¯
氎溶æ§ã§ãããé µçŽ åã³æšèå€ãšåæ§ã®æ¹æ³ã§å
æã«å°å ¥ããããäžæŽ»æ§åã®å¥ã®æ¹æ³ã¯ãå ¬ç¥æ
è¡ã«ããããããè£é µçŽ ãé€å»ããããšã«ããã
ãã®ããã«ããŠåŸãããã¢ãé µçŽ ãå°å ¥ããè£é µ
çŽ ããªããœãŒã å€éšã®æº¶æ¶²ã«ä¿æããã溶解æã
äžæŽ»æ§åœ¢ã®ã¢ãé µçŽ ã§ããå°å ¥ãããé µçŽ ãè£é µ
çŽ ãšäžç·ã«ãªã€ãŠæŽ»æ§é µçŽ ã圢æããã
å¢å
éšã«å«æãããæšèå€ã¯å€ãã®æ€åºå¯èœãª
æšèå€ã®ãã¡ã®ïŒã€ã§ãããããã¯ããšãã°éé
å®çã«ãæŸå°æ§åäœäœãé µçŽ ïŒé µçŽ 䜿çšã®éã«
ã¯ãæšèå€ãšæº¶è§£å€ã¯åãé µçŽ ã§ãããããŸãã¯
ç°ãªã€ãé µçŽ ã§ãããïŒãã¯ãã¢ã²ã³ïŒåžçææ
ãŸãã¯è¢å ç©è³ªïŒãçºå ååç©ããªã³å æ§ååç©ã
ã¹ãã³ã©ãã«çãå«ãããã®ãããªæ€åºå¯èœãªæš
èå€ããã³æšèå€æž¬å®æ¹æ³ã¯äžè¬ã«åœè©²åéã§å ¬
ç¥ã§ããããã®ç¹ã«ã€ããŠã®ããã«è©³çŽ°ã¯æ¬çºæ
ã®å®å šãªç解ã«ã¯å¿ èŠã§ãªããšæãããã䜿çšã
ããæšèå€ã®å¥œãŸããåã¯æ¬¡ã®ããã§ããïŒ ïœ åžå ä¿æ°ã®é«ãææãããšãã°ã¹ã«ãããŒã
ãã³ïŒ¢é ãã¿ãã·ã¢ãã³ããã©ã¹ã«ãã³é žããª
ããµãžã³ïŒããŒã¯ãã¬ãŒãã ïœ è¢å ææãããšãã°ã«ã«ããã·ãã«ãªã¬ã»ã€
ã³ããŠãŒãããŠã ããã³ãã«ããŠã ã®ææ©ãã¬
ãŒããæ§ã ãªã¯ããªã³ããã³ããŒããã³ã ïœ æº¶è§£é µçŽ ä»¥å€ã®é µçŽ ãããšãã°è¥¿æŽã¯ãµãã®
ãã«ãªãã·ããŒãŒãããã¯æº¶è§£åŸã«æ¯è²ãè¢
å ãçºå ãŸãã¯é»æ°ååŠïŒé»æµæž¬å®ïŒè£ 眮ã§æž¬
å®ãããã
æšèå€ã®ãã¡ã®ïŒã€ã§ãããããã¯ããšãã°éé
å®çã«ãæŸå°æ§åäœäœãé µçŽ ïŒé µçŽ 䜿çšã®éã«
ã¯ãæšèå€ãšæº¶è§£å€ã¯åãé µçŽ ã§ãããããŸãã¯
ç°ãªã€ãé µçŽ ã§ãããïŒãã¯ãã¢ã²ã³ïŒåžçææ
ãŸãã¯è¢å ç©è³ªïŒãçºå ååç©ããªã³å æ§ååç©ã
ã¹ãã³ã©ãã«çãå«ãããã®ãããªæ€åºå¯èœãªæš
èå€ããã³æšèå€æž¬å®æ¹æ³ã¯äžè¬ã«åœè©²åéã§å ¬
ç¥ã§ããããã®ç¹ã«ã€ããŠã®ããã«è©³çŽ°ã¯æ¬çºæ
ã®å®å šãªç解ã«ã¯å¿ èŠã§ãªããšæãããã䜿çšã
ããæšèå€ã®å¥œãŸããåã¯æ¬¡ã®ããã§ããïŒ ïœ åžå ä¿æ°ã®é«ãææãããšãã°ã¹ã«ãããŒã
ãã³ïŒ¢é ãã¿ãã·ã¢ãã³ããã©ã¹ã«ãã³é žããª
ããµãžã³ïŒããŒã¯ãã¬ãŒãã ïœ è¢å ææãããšãã°ã«ã«ããã·ãã«ãªã¬ã»ã€
ã³ããŠãŒãããŠã ããã³ãã«ããŠã ã®ææ©ãã¬
ãŒããæ§ã ãªã¯ããªã³ããã³ããŒããã³ã ïœ æº¶è§£é µçŽ ä»¥å€ã®é µçŽ ãããšãã°è¥¿æŽã¯ãµãã®
ãã«ãªãã·ããŒãŒãããã¯æº¶è§£åŸã«æ¯è²ãè¢
å ãçºå ãŸãã¯é»æ°ååŠïŒé»æµæž¬å®ïŒè£ 眮ã§æž¬
å®ãããã
äžè¿°ããããã«ãæ€å®ã§äœ¿çšããããã¬ãŒãµãŒ
ã¯æŽ»æ§åœ¢ã«ãŠçµåããŠããå¢æº¶è§£å€ãæãããªã¬
ã³ãã§ãããå¢æº¶è§£å€ã¯äžè¿°ãããããªåã®ãã®
ã§ããããã¬ãŒãµãŒè£œé ã«äœ¿çšããããªã¬ã³ã
ã¯ãå®æœãããæ€å®ã«äŸåãããããªãã¡ãããš
ãã°æåãŸãã¯ãããã³ã§ãã被æ€ç©è³ªã«ã€ããŠ
ã®æ€å®ã§ããå Žåã«ã¯ããã¬ãŒãµãŒã®ãªã¬ã³ãéš
åã¯è¢«æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ã§ããã
ã¯æŽ»æ§åœ¢ã«ãŠçµåããŠããå¢æº¶è§£å€ãæãããªã¬
ã³ãã§ãããå¢æº¶è§£å€ã¯äžè¿°ãããããªåã®ãã®
ã§ããããã¬ãŒãµãŒè£œé ã«äœ¿çšããããªã¬ã³ã
ã¯ãå®æœãããæ€å®ã«äŸåãããããªãã¡ãããš
ãã°æåãŸãã¯ãããã³ã§ãã被æ€ç©è³ªã«ã€ããŠ
ã®æ€å®ã§ããå Žåã«ã¯ããã¬ãŒãµãŒã®ãªã¬ã³ãéš
åã¯è¢«æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ã§ããã
ããã§äœ¿çšãããŠããâé©åœãªé¡äŒŒç©âãšã¯ã
被æ€ç©è³ªã®é¡äŒŒç©ã«é¢ããå Žåã被æ€ç©è³ªã®é¡äŒŒ
ç©ãæ€äœã«äœ¿çšããã被æ€ç©è³ªã«å¯Ÿãããã€ã³ã
ãŒã«çµåããããšãæå³ããã被æ€ç©è³ªãæäœã®
å Žåã«ã¯ããã¬ãŒãµãŒã®ãªã¬ã³ãéšåã¯æäœã«çµ
åããæåãŸãã¯è¢«æ€ç©è³ªã«å¯Ÿå¿ããŠåŒãåºãã
ãæäœã§ããã
被æ€ç©è³ªã®é¡äŒŒç©ã«é¢ããå Žåã被æ€ç©è³ªã®é¡äŒŒ
ç©ãæ€äœã«äœ¿çšããã被æ€ç©è³ªã«å¯Ÿãããã€ã³ã
ãŒã«çµåããããšãæå³ããã被æ€ç©è³ªãæäœã®
å Žåã«ã¯ããã¬ãŒãµãŒã®ãªã¬ã³ãéšåã¯æäœã«çµ
åããæåãŸãã¯è¢«æ€ç©è³ªã«å¯Ÿå¿ããŠåŒãåºãã
ãæäœã§ããã
ãã¬ãŒãµãŒã®ãªã¬ã³ãéšåã¯ãã€ã³ããŒãŸãã¯
被æ€ç©è³ªã®ïŒã€ã«çµåãããããªãã¡ãããšãã°
ããããâãµã³ãã€ããâåæ€å®æ³ã«ãããŠã¯ã
被æ€ç©è³ªã¯ãã€ã³ããŒã«çµåããã¬ãŒãµãŒã¯ãã®
被æ€ç©è³ªã«çµåããŠããããããã«ãã被æ€ç©è³ª
ãä»ããŠãã€ã³ããŒã«çµåãããã¬ãŒãµãŒã®éã¯
æ€äœäžã®è¢«æ€ç©è³ªã®éã«ããã
被æ€ç©è³ªã®ïŒã€ã«çµåãããããªãã¡ãããšãã°
ããããâãµã³ãã€ããâåæ€å®æ³ã«ãããŠã¯ã
被æ€ç©è³ªã¯ãã€ã³ããŒã«çµåããã¬ãŒãµãŒã¯ãã®
被æ€ç©è³ªã«çµåããŠããããããã«ãã被æ€ç©è³ª
ãä»ããŠãã€ã³ããŒã«çµåãããã¬ãŒãµãŒã®éã¯
æ€äœäžã®è¢«æ€ç©è³ªã®éã«ããã
æ€å®ã®äžè¿°ã®åããã³ä»ã®ãã®ã¯æ¬æ现æžã«èš
èŒã®äºé ããåœæ¥è ã«ãšã€ãŠæããã§ããã
èŒã®äºé ããåœæ¥è ã«ãšã€ãŠæããã§ããã
ãã¬ãŒãµãŒã®ãªã¬ã³ãéšåã¯ãïŒã€ã®ååç©ã
ä»ã®ååç©ãšçµåããããšã«ã€ããŠåœè©²åéã§äž
è¬ã«å ¬ç¥ã®æ¹æ³ã«ãã溶解å€ãšçµåãããããªã
ã¡ãããšãã°æº¶è§£å€ã¯ãå ±æçµåãèªå°åã掻æ§
åçã«ãããã¬ãŒãµãŒã®ãªã¬ã³ãéšåãžçµåã
ãã
ä»ã®ååç©ãšçµåããããšã«ã€ããŠåœè©²åéã§äž
è¬ã«å ¬ç¥ã®æ¹æ³ã«ãã溶解å€ãšçµåãããããªã
ã¡ãããšãã°æº¶è§£å€ã¯ãå ±æçµåãèªå°åã掻æ§
åçã«ãããã¬ãŒãµãŒã®ãªã¬ã³ãéšåãžçµåã
ãã
溶解å€ã¯ãã«ãããªã³ã°ååç©ãŸãã¯ã¹ããŒãµ
ãŒååç©ïŒãã¬ãŒãµãŒã®å ç«åå¿æ§ãŸãã¯æº¶è§£å€
ã®å¢æº¶è§£æŽ»æ§ãæãªããªããã®ïŒã䜿çšããããš
ã«ãããã¬ãŒãµãŒã®ãªã¬ã³ãéšåã«çµåããŠãã
ããåœè©²åéã§å ¬ç¥ã®ããã«ãã«ãããªã³ã°åå
ç©ã¯ïŒã€ã®åå¿æ§å®èœåºãæããå®èœåºã®ïŒã€ã¯
ãã¬ãŒãµãŒã®ãªã¬ã³ãéšåã®å®èœåºãšåå¿ããã
é£çµããããšãã§ããä»ã®ïŒã€ã¯å¢æº¶è§£å€ã®å®èœ
åºãšåå¿ãããé£çµããããšãã§ãããããšã
ã°ãã¹ããŒãµãŒãŸãã¯ã«ãããªã³ã°ååç©ã¯å°ãª
ããšãïŒã€ã®åå¿æ§çœ®æåºãå«ã¿ãã«ã«ããã·
ã«ãã€ãœã·ã¢ããŒããã€ãœããªã·ã¢ããŒããã¢ã
ããããªãŒã«ãããããã·ãã¹ã«ããã«ãã«ã«ã
ãã«çã®çœ®æåºã®ãããããå«ãã§ããŠãããã
ãã¯äºãã«çµåãã¹ããªã¬ã³ãããã³æº¶è§£å€ã«å
åšããå®èœåºã«ããããšã¯æããã§ããã
ãŒååç©ïŒãã¬ãŒãµãŒã®å ç«åå¿æ§ãŸãã¯æº¶è§£å€
ã®å¢æº¶è§£æŽ»æ§ãæãªããªããã®ïŒã䜿çšããããš
ã«ãããã¬ãŒãµãŒã®ãªã¬ã³ãéšåã«çµåããŠãã
ããåœè©²åéã§å ¬ç¥ã®ããã«ãã«ãããªã³ã°åå
ç©ã¯ïŒã€ã®åå¿æ§å®èœåºãæããå®èœåºã®ïŒã€ã¯
ãã¬ãŒãµãŒã®ãªã¬ã³ãéšåã®å®èœåºãšåå¿ããã
é£çµããããšãã§ããä»ã®ïŒã€ã¯å¢æº¶è§£å€ã®å®èœ
åºãšåå¿ãããé£çµããããšãã§ãããããšã
ã°ãã¹ããŒãµãŒãŸãã¯ã«ãããªã³ã°ååç©ã¯å°ãª
ããšãïŒã€ã®åå¿æ§çœ®æåºãå«ã¿ãã«ã«ããã·
ã«ãã€ãœã·ã¢ããŒããã€ãœããªã·ã¢ããŒããã¢ã
ããããªãŒã«ãããããã·ãã¹ã«ããã«ãã«ã«ã
ãã«çã®çœ®æåºã®ãããããå«ãã§ããŠãããã
ãã¯äºãã«çµåãã¹ããªã¬ã³ãããã³æº¶è§£å€ã«å
åšããå®èœåºã«ããããšã¯æããã§ããã
ããã«ä»£ãããå¢æº¶è§£å€ã¯çŽæ¥ãªã¬ã³ãã«çµå
ããŠããããããªãã¡ãããšãã°ãã¬ãŒãµãŒã®ãª
ã¬ã³ãéšåãã¢ãã眮æåºãæãããã¬ãŒãµãŒã®
å¢æº¶è§£å€éšåãã«ã«ããã«ãŸãã¯ã«ã«ããã·ã«çœ®
æåºãæããå Žåã«ã¯ããªã¬ã³ããšå¢æº¶è§£å€ã¯ã
åœè©²åéã§å ¬ç¥ã®æ¹æ³ããšãã°æŽ»æ§ãšã¹ãã«æ³ã«
ããçžäºã«çŽæ¥çµåããã
ããŠããããããªãã¡ãããšãã°ãã¬ãŒãµãŒã®ãª
ã¬ã³ãéšåãã¢ãã眮æåºãæãããã¬ãŒãµãŒã®
å¢æº¶è§£å€éšåãã«ã«ããã«ãŸãã¯ã«ã«ããã·ã«çœ®
æåºãæããå Žåã«ã¯ããªã¬ã³ããšå¢æº¶è§£å€ã¯ã
åœè©²åéã§å ¬ç¥ã®æ¹æ³ããšãã°æŽ»æ§ãšã¹ãã«æ³ã«
ããçžäºã«çŽæ¥çµåããã
æ€å®ã«äœ¿çšããããã€ã³ããŒã¯è¢«æ€ç©è³ªã«å¯Ÿã
ç¹ç°çãªãã®ã®ïŒã€ã§ããã被æ€ç©è³ªãæåãŸã
ã¯ãããã³ã®å Žåã«ã¯ããã€ã³ããŒã¯æäœãŸãã¯
被æ€ç©è³ªã«ç¹ç°çãªå€©ç¶ç£åºãã€ã³ããŒã§ãã
ãã被æ€ç©è³ªãæäœã®å Žåã«ã¯ãæ€äœã«äœ¿çšãã
ããã€ã³ããŒã¯æ€å®ãããã¹ãæäœã«å¯Ÿå¿ããŠåŒ
ãåºãããæåãŸãã¯æäœã§ãããããã«ããã
ã€ã³ããŒã¯è¢«æ€ç©è³ªã«ç¹ç°çã§ããã
ç¹ç°çãªãã®ã®ïŒã€ã§ããã被æ€ç©è³ªãæåãŸã
ã¯ãããã³ã®å Žåã«ã¯ããã€ã³ããŒã¯æäœãŸãã¯
被æ€ç©è³ªã«ç¹ç°çãªå€©ç¶ç£åºãã€ã³ããŒã§ãã
ãã被æ€ç©è³ªãæäœã®å Žåã«ã¯ãæ€äœã«äœ¿çšãã
ããã€ã³ããŒã¯æ€å®ãããã¹ãæäœã«å¯Ÿå¿ããŠåŒ
ãåºãããæåãŸãã¯æäœã§ãããããã«ããã
ã€ã³ããŒã¯è¢«æ€ç©è³ªã«ç¹ç°çã§ããã
äžè¿°ã®ããã«ããã€ã³ããŒãšçµåããªããã¬ãŒ
ãµãŒããäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åºå¯èœãªæšèå€
ã®äž¡æ¹ãå«ãå¢ãšæ¥è§Šãããããã«ããããšã«ã
ãæ€å®ãè¡ãªããããã¯åºçžæ¯æäœã«ãã€ã³ããŒ
ãæ¯æãããããšã«ããéœåè¯ãå®æœããããã
ãã«ããçµåãã¬ãŒãµãŒã¯æ€å®åªäœäžãèªç±ã«ç§»
åããŠæ€å®ã§çšããããå¢ãšæ¥è§Šããããšããª
ãã
ãµãŒããäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åºå¯èœãªæšèå€
ã®äž¡æ¹ãå«ãå¢ãšæ¥è§Šãããããã«ããããšã«ã
ãæ€å®ãè¡ãªããããã¯åºçžæ¯æäœã«ãã€ã³ããŒ
ãæ¯æãããããšã«ããéœåè¯ãå®æœããããã
ãã«ããçµåãã¬ãŒãµãŒã¯æ€å®åªäœäžãèªç±ã«ç§»
åããŠæ€å®ã§çšããããå¢ãšæ¥è§Šããããšããª
ãã
ããªãã¡ãããšãã°ãã€ã³ããŒã¯ãåžçãå
±æ
çµåã掻æ§åçãå«ãåœè©²åéã§å ¬ç¥ã®æ¹æ³ã«ã
ãåºçžè¡šé¢ã«æ¯æãããããã€ã³ããŒã«å¯Ÿããåº
äœæ¯æäœã¯æ€å®ã®åŠšããšãªããªãåºãç¯å²ã®åºçž
æ¯æäœã®ãã¡ã®ïŒã€ã§ãããããšãã°ééå®ç
ã«ãããªããŒæ¯æäœããšãã°ããªãããã¬ã³ãã
ãªã¹ãã¬ã³ãããªãšãã¬ã³çïŒã¬ã©ã¹ã现è现
èïŒã€ãªã³äº€ææš¹èçãå«ãŸããããã®ãããªåº
çžæ¯æäœã¯åœè©²åéã§å ¬ç¥ã§ããããã®ç¹ã«ã€ã
ãŠããã«è©³çŽ°ãªäºé ã¯æ¬çºæãå®å šã«ç解ããã«
ã¯å¿ èŠãªããšæãããã
çµåã掻æ§åçãå«ãåœè©²åéã§å ¬ç¥ã®æ¹æ³ã«ã
ãåºçžè¡šé¢ã«æ¯æãããããã€ã³ããŒã«å¯Ÿããåº
äœæ¯æäœã¯æ€å®ã®åŠšããšãªããªãåºãç¯å²ã®åºçž
æ¯æäœã®ãã¡ã®ïŒã€ã§ãããããšãã°ééå®ç
ã«ãããªããŒæ¯æäœããšãã°ããªãããã¬ã³ãã
ãªã¹ãã¬ã³ãããªãšãã¬ã³çïŒã¬ã©ã¹ã现è现
èïŒã€ãªã³äº€ææš¹èçãå«ãŸããããã®ãããªåº
çžæ¯æäœã¯åœè©²åéã§å ¬ç¥ã§ããããã®ç¹ã«ã€ã
ãŠããã«è©³çŽ°ãªäºé ã¯æ¬çºæãå®å šã«ç解ããã«
ã¯å¿ èŠãªããšæãããã
åœè©²åéã§å
¬ç¥ã®ããã«ãåºçžæ¯æäœã¯çš®ã
ã®
圢æ ããšãã°è©Šéšç®¡ãã·ãŒãããŸãã¯æ¿ãåºçžç²
åç¶çã§ããã
圢æ ããšãã°è©Šéšç®¡ãã·ãŒãããŸãã¯æ¿ãåºçžç²
åç¶çã§ããã
ãã€ã³ããŒã¯æ€å®ã«ãããŠæªæ¯æ圢æ
ã§çšãã
ããéé¢ãã¬ãŒãµãŒãšå¢ãšãæ¥è§Šãããåã«çµå
ããã³éé¢éšåãçžäºã«åé¢ããããã«ããŠãè¯
ãããšãç解ãããã¹ãã§ããã
ããéé¢ãã¬ãŒãµãŒãšå¢ãšãæ¥è§Šãããåã«çµå
ããã³éé¢éšåãçžäºã«åé¢ããããã«ããŠãè¯
ãããšãç解ãããã¹ãã§ããã
åºçžã«æ¯æããããã€ã³ããŒã䜿çšããå Žåã
éçµåãã¬ãŒãµãŒïŒéé¢ãã¬ãŒãµãŒïŒããã®å éš
ã«æšèå€ãšäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšãå«ãå¢ãšæ¥è§Š
ããåã«ãçµåãããã¬ãŒãµãŒãæããæ¯æãã€
ã³ããŒãåé¢ããå¿ èŠãããããšãããã
éçµåãã¬ãŒãµãŒïŒéé¢ãã¬ãŒãµãŒïŒããã®å éš
ã«æšèå€ãšäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšãå«ãå¢ãšæ¥è§Š
ããåã«ãçµåãããã¬ãŒãµãŒãæããæ¯æãã€
ã³ããŒãåé¢ããå¿ èŠãããããšãããã
ãŸããã®ä»ã«ãããšãã°ãã€ã³ããŒã管ã®å£ã«
æ¯æãããæã«ã¯ãçµåãã¬ãŒãµãŒã¯æ€å®å¹å°äž
ãèªç±ã«ç§»åããããšããªãããããã€ãŠéé¢ã
ã¬ãŒãµãŒãšäžè¿°ããåã®å¢ãšãæ¥è§Šãããåã«ã
çµåãããã¬ãŒãµãŒããéé¢ãã¬ãŒãµãŒãåé¢ã
ãå¿ èŠããªãå Žåãããã
æ¯æãããæã«ã¯ãçµåãã¬ãŒãµãŒã¯æ€å®å¹å°äž
ãèªç±ã«ç§»åããããšããªãããããã€ãŠéé¢ã
ã¬ãŒãµãŒãšäžè¿°ããåã®å¢ãšãæ¥è§Šãããåã«ã
çµåãããã¬ãŒãµãŒããéé¢ãã¬ãŒãµãŒãåé¢ã
ãå¿ èŠããªãå Žåãããã
æ¯æããããã€ã³ããŒãçšããå Žåã«ã¯ã被æ€
ç©è³ªïŒæ€å®ãããã¹ããªã¬ã³ãïŒãå«ãæ€äœãšã
ã¬ãŒãµãŒãšããæ€åºå¯èœãªæšèå€ãšäžæŽ»æ§åœ¢ã®å¢
溶解å€ã®äž¡æ¹ãå«ãå¢ãå ¥ã€ãæ€å®å¹å°äžã§æ¯æ
ããããã€ã³ããŒã®ååšäžã«ã€ã³ããŠããŒããã
ããšãã§ããããã®ãããªæ¹æ³ã«ãããŠã溶解é
床ã¯ãçµåãã¬ãŒãµãŒãæ€å®å¹å°äžã§èªç±ã«åã
ãªãã®ã§éé¢ãã¬ãŒãµãŒã®æ¿åºŠã®é¢æ°ã§ãããå¢
ã¯æ€äœå¹å°äžãèªç±ã«ç§»åãããããã€ãŠçè«ç
ã«ã¯å¢ã移åããŠçµåãã¬ãŒãµãŒãšæ¥è§Šãçµåã
ã¬ãŒãµãŒã«ããå¢ã®æº¶è§£ãéå§ããããšã«ãªããš
ã¯ãããå®éçã«ã¯ãã®ãããªããšãèµ·ããå¯èœ
æ§ã¯å°ãªããå¢ã®æº¶è§£é床ã¯æ€äœå¹å°äžã®éé¢ã
ã¬ãŒãµãŒã®æ¿åºŠé¢æ°ã§ã«ããæåã«ã€ã³ããŠããŒ
ã·ãšã³ããŠéé¢ãã¬ãŒãµãŒéšåãšçµåãã¬ãŒãµãŒ
éšåã圢æããåŸå¢ãå ããããšãæå©ãªå Žåã
ãããããããšã¯å¥ã«ãçµåéšåããéé¢éšåã
åé¢ãã次ãã§ãã®éé¢éšåãæ€åºå¯èœãªæšèå€
ãšäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšãå«ãå¢ãšæ¥è§Šãããã
ãšãæå©ãªå Žåãããã
ç©è³ªïŒæ€å®ãããã¹ããªã¬ã³ãïŒãå«ãæ€äœãšã
ã¬ãŒãµãŒãšããæ€åºå¯èœãªæšèå€ãšäžæŽ»æ§åœ¢ã®å¢
溶解å€ã®äž¡æ¹ãå«ãå¢ãå ¥ã€ãæ€å®å¹å°äžã§æ¯æ
ããããã€ã³ããŒã®ååšäžã«ã€ã³ããŠããŒããã
ããšãã§ããããã®ãããªæ¹æ³ã«ãããŠã溶解é
床ã¯ãçµåãã¬ãŒãµãŒãæ€å®å¹å°äžã§èªç±ã«åã
ãªãã®ã§éé¢ãã¬ãŒãµãŒã®æ¿åºŠã®é¢æ°ã§ãããå¢
ã¯æ€äœå¹å°äžãèªç±ã«ç§»åãããããã€ãŠçè«ç
ã«ã¯å¢ã移åããŠçµåãã¬ãŒãµãŒãšæ¥è§Šãçµåã
ã¬ãŒãµãŒã«ããå¢ã®æº¶è§£ãéå§ããããšã«ãªããš
ã¯ãããå®éçã«ã¯ãã®ãããªããšãèµ·ããå¯èœ
æ§ã¯å°ãªããå¢ã®æº¶è§£é床ã¯æ€äœå¹å°äžã®éé¢ã
ã¬ãŒãµãŒã®æ¿åºŠé¢æ°ã§ã«ããæåã«ã€ã³ããŠããŒ
ã·ãšã³ããŠéé¢ãã¬ãŒãµãŒéšåãšçµåãã¬ãŒãµãŒ
éšåã圢æããåŸå¢ãå ããããšãæå©ãªå Žåã
ãããããããšã¯å¥ã«ãçµåéšåããéé¢éšåã
åé¢ãã次ãã§ãã®éé¢éšåãæ€åºå¯èœãªæšèå€
ãšäžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšãå«ãå¢ãšæ¥è§Šãããã
ãšãæå©ãªå Žåãããã
æ¬çºæã®æ€å®ã¯çš®ã
ã®è¢«æ€ç©è³ªã枬å®ããã®ã«
çšããããäžè¬ã«æ€å®ãããã¹ãç©è³ªäžã«äœæ¿åºŠ
ã§èŠããããããªè¢«æ€ç©è³ªã«ã€ããŠç¹ã«é©ããã
ãã®ãããªè¢«æ€ç©è³ªã®ä»£è¡šçäŸã¯æ¬¡ã®ããã§ã
ãïŒåŒ·å¿é ç³äœãããšãã°ãžãŽãã·ã³ããã³ãžã®
ããã·ã³ãæããæ¯å€ãããšãã°ããªãã€ãªã³ã
æçç©è³ªãããšãã°ã²ã³ã¿ãã€ã·ã³ããã³ããã©
ãã€ã·ã³ãæè «çè¬ãããšãã°ã¡ãœãã¬ãã¶ãŒ
ããæããããè¬ãããšãã°ããšããã«ãã¿ãŒ
ã«ãã«ã«ãã¡ã¶ãã³ããã³ãã«ãã«é žïŒvalparic
acidïŒãæäžæŽèè¬ãããšãã°ãªãã«ã€ã³ããã³
ãããžã³ããã«ã¢ã³ãããšãã°T4ãT3ãhCGã
TSHããã³çš®ã ã®ã¹ããã€ããæ¬çºæã¯ããã
ã®ä»£è¡šäŸã«éå®ãããªãã
çšããããäžè¬ã«æ€å®ãããã¹ãç©è³ªäžã«äœæ¿åºŠ
ã§èŠããããããªè¢«æ€ç©è³ªã«ã€ããŠç¹ã«é©ããã
ãã®ãããªè¢«æ€ç©è³ªã®ä»£è¡šçäŸã¯æ¬¡ã®ããã§ã
ãïŒåŒ·å¿é ç³äœãããšãã°ãžãŽãã·ã³ããã³ãžã®
ããã·ã³ãæããæ¯å€ãããšãã°ããªãã€ãªã³ã
æçç©è³ªãããšãã°ã²ã³ã¿ãã€ã·ã³ããã³ããã©
ãã€ã·ã³ãæè «çè¬ãããšãã°ã¡ãœãã¬ãã¶ãŒ
ããæããããè¬ãããšãã°ããšããã«ãã¿ãŒ
ã«ãã«ã«ãã¡ã¶ãã³ããã³ãã«ãã«é žïŒvalparic
acidïŒãæäžæŽèè¬ãããšãã°ãªãã«ã€ã³ããã³
ãããžã³ããã«ã¢ã³ãããšãã°T4ãT3ãhCGã
TSHããã³çš®ã ã®ã¹ããã€ããæ¬çºæã¯ããã
ã®ä»£è¡šäŸã«éå®ãããªãã
æ¬çºæã®æ€å®æ³ã®äžé¢ã«ããã°ã被æ€ç©è³ªãå«
ããŸãã¯å«ããšæãããæ€äœããå¢æº¶è§£å€ãšçµå
ãã被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ãããªãã
ã¬ãŒãµãŒããã³è¢«æ€ç©è³ªãšãã¬ãŒãµãŒã®äž¡æ¹ã«ç¹
ç°çãªãã€ã³ããŒãšãšãã«ã€ã³ããŠããŒãããã
ã€ã³ããŠããŒãã«ãããã€ã³ããŒã«ãããçµåç®
æããã¬ãŒãµãŒãšè¢«æ€ç©è³ªãšã§ç«¶åããçµæãšãª
ãããã€ã³ããŒã«çµåãããã¬ãŒãµãŒã®éã¯æ€äœ
äžã®è¢«æ€ç©è³ªéãšéæ¯äŸããã
ããŸãã¯å«ããšæãããæ€äœããå¢æº¶è§£å€ãšçµå
ãã被æ€ç©è³ªãŸãã¯ãã®é©åœãªé¡äŒŒç©ãããªãã
ã¬ãŒãµãŒããã³è¢«æ€ç©è³ªãšãã¬ãŒãµãŒã®äž¡æ¹ã«ç¹
ç°çãªãã€ã³ããŒãšãšãã«ã€ã³ããŠããŒãããã
ã€ã³ããŠããŒãã«ãããã€ã³ããŒã«ãããçµåç®
æããã¬ãŒãµãŒãšè¢«æ€ç©è³ªãšã§ç«¶åããçµæãšãª
ãããã€ã³ããŒã«çµåãããã¬ãŒãµãŒã®éã¯æ€äœ
äžã®è¢«æ€ç©è³ªéãšéæ¯äŸããã
çµåããã³éé¢æåãäºãã«åé¢ãã次ãã§é
é¢éšåãããã®å éšã«æšèå€ãšäžæŽ»æ§åœ¢å¢æº¶è§£å€
ã®äž¡æ¹ãå«ãå¢ã«ããã®å¢ãæ©ãã«ç Žå£ãããã®
ã劚ããæ¡ä»¶äžïŒå¢ã¯å¢å€åŽã®æº¶è§£å€ãšæ¥è§Šãã
ããšã«ãã€ãŠã®ã¿ç Žå£ãããïŒã§æ¥è§Šããããæ€
å®ã®ãã®éšåã¯ãéåžžããªã¹ã¢ãŒã©ãªãã€ã®å¢ã§
ç匵åãããé©åœãªç·©è¡ãæ°Žæ§å¹å°ã§è¡ãªãã
ãããããã€ãŠã枩床ãPHããã³ã€ãªã³æ¿åºŠã®æ¡
件ã¯ãå¢ã®æ©æç Žå£ãé²ãããã«èª¿ç¯ãããã
é¢éšåãããã®å éšã«æšèå€ãšäžæŽ»æ§åœ¢å¢æº¶è§£å€
ã®äž¡æ¹ãå«ãå¢ã«ããã®å¢ãæ©ãã«ç Žå£ãããã®
ã劚ããæ¡ä»¶äžïŒå¢ã¯å¢å€åŽã®æº¶è§£å€ãšæ¥è§Šãã
ããšã«ãã€ãŠã®ã¿ç Žå£ãããïŒã§æ¥è§Šããããæ€
å®ã®ãã®éšåã¯ãéåžžããªã¹ã¢ãŒã©ãªãã€ã®å¢ã§
ç匵åãããé©åœãªç·©è¡ãæ°Žæ§å¹å°ã§è¡ãªãã
ãããããã€ãŠã枩床ãPHããã³ã€ãªã³æ¿åºŠã®æ¡
件ã¯ãå¢ã®æ©æç Žå£ãé²ãããã«èª¿ç¯ãããã
ããªãã¡ãããšãã°ãªã¹ã¢ãŒã©ãªãã€ã®å¢ã§ç
匵åãããæ°Žæ§ç·©è¡å¹å°ãæäŸããããäžè¬ã«ã
ç·©è¡æ¶²ã¯PHïŒãïŒãããããã
匵åãããæ°Žæ§ç·©è¡å¹å°ãæäŸããããäžè¬ã«ã
ç·©è¡æ¶²ã¯PHïŒãïŒãããããã
ãã¬ãŒãµãŒã®æº¶è§£å€éšåãšæ¥è§Šããçµæãå¢ã
ç Žå£ããå¥ã®æº¶è§£å€ãæŸåºãããã®æŸåºããã掻
æ§åœ¢ã®æº¶è§£å€ãšãšãã«æšèå€ãæŸåºããïŒããšã
ã°ãæ€äœå¹å°ã¯ã«ã«ã·ãŠã ãå«ã¿ãäžæ¹å¢ã®å éš
ã¯ã«ã«ã·ãŠã é»å®³å€ãå«ãïŒã掻æ§æº¶è§£å€ã®æŸåº
ã¯äžè¿°ããããã«æ®µéçãŸãã¯å¢å¹ å¹æãçã
ããæšèå€ãå¹å°ãžæŸåºãããé床ã¯ååšããã
ã¬ãŒãµãŒã®æ¿åºŠã«åŸãããã¬ãŒãµãŒã®éãå¢ãã
ãšå¹å°ãžã®æšèå€æŸåºé床ãäžæããçµæãšãª
ããããªãã¡ãæšèå€ãå¹å°ãžæŸåºãããé床ãŸ
ãã¯ããã«ä»£ãã€ãŠäžå®æéåŸã®å¹å°äžã®æšèå€
éã枬å®ãããããã®å€ã被æ€ç©è³ªïŒæ¢ç¥æ¿åºŠã
æããæšæºè¢«æ€ç©è³ªïŒã®æ¢ç¥éãçšããŠåãæ¹æ³
ã§åŸãããå€ãšæ¯èŒããããšã«ãã€ãŠãæ€äœäžã«
ååšãã被æ€ç©è³ªã®éã®æž¬å®å€ãåŸãããã
ç Žå£ããå¥ã®æº¶è§£å€ãæŸåºãããã®æŸåºããã掻
æ§åœ¢ã®æº¶è§£å€ãšãšãã«æšèå€ãæŸåºããïŒããšã
ã°ãæ€äœå¹å°ã¯ã«ã«ã·ãŠã ãå«ã¿ãäžæ¹å¢ã®å éš
ã¯ã«ã«ã·ãŠã é»å®³å€ãå«ãïŒã掻æ§æº¶è§£å€ã®æŸåº
ã¯äžè¿°ããããã«æ®µéçãŸãã¯å¢å¹ å¹æãçã
ããæšèå€ãå¹å°ãžæŸåºãããé床ã¯ååšããã
ã¬ãŒãµãŒã®æ¿åºŠã«åŸãããã¬ãŒãµãŒã®éãå¢ãã
ãšå¹å°ãžã®æšèå€æŸåºé床ãäžæããçµæãšãª
ããããªãã¡ãæšèå€ãå¹å°ãžæŸåºãããé床ãŸ
ãã¯ããã«ä»£ãã€ãŠäžå®æéåŸã®å¹å°äžã®æšèå€
éã枬å®ãããããã®å€ã被æ€ç©è³ªïŒæ¢ç¥æ¿åºŠã
æããæšæºè¢«æ€ç©è³ªïŒã®æ¢ç¥éãçšããŠåãæ¹æ³
ã§åŸãããå€ãšæ¯èŒããããšã«ãã€ãŠãæ€äœäžã«
ååšãã被æ€ç©è³ªã®éã®æž¬å®å€ãåŸãããã
çµæçã«ä¿¡å·åŒ·åºŠã®å¢å ã枬å®ãããããŸãã¯
æçµç¹æ³ãããªãã¡åå¿ãäžå®æéé²ãŸã次ãã§
åæ¢ïŒããšãã°PHå¢å ã«ããïŒãããè²ïŒãŸãã¯
å Žåã«ãã€ãŠã¯è¢å ãããã¯çºå ïŒã枬å®ããæ¹
æ³ã«ãããé床ãåçã«æž¬å®ããããšãã§ããã
åå¿é床ãéããªãã°æçµç¹ã§ã®ä¿¡å·ã匷ããªã
ã§ãããã
æçµç¹æ³ãããªãã¡åå¿ãäžå®æéé²ãŸã次ãã§
åæ¢ïŒããšãã°PHå¢å ã«ããïŒãããè²ïŒãŸãã¯
å Žåã«ãã€ãŠã¯è¢å ãããã¯çºå ïŒã枬å®ããæ¹
æ³ã«ãããé床ãåçã«æž¬å®ããããšãã§ããã
åå¿é床ãéããªãã°æçµç¹ã§ã®ä¿¡å·ã匷ããªã
ã§ãããã
æ€å®ã«çšããããæ€äœå®¹éã¯ãæšèå€ã®æŸåºé
床ãééãªãæ©ããªãã®ãé²ãããã«ãããªãã¡
çµæçå€åãæ€åºå¯èœãªé床ã«ãªãããã«éžæã
ãããããªãã¡ãäºæããã被æ€ç©è³ªæ¿åºŠãäžæ
ãããšãæ€äœå®¹éã¯é床ãæ€åºå¯èœã«å€åããã
ãã«æžå°ããã
床ãééãªãæ©ããªãã®ãé²ãããã«ãããªãã¡
çµæçå€åãæ€åºå¯èœãªé床ã«ãªãããã«éžæã
ãããããªãã¡ãäºæããã被æ€ç©è³ªæ¿åºŠãäžæ
ãããšãæ€äœå®¹éã¯é床ãæ€åºå¯èœã«å€åããã
ãã«æžå°ããã
å¥ã®å®æœæ
æ§ã«ããã°ããã€ã³ããŒã¯ç®¡å£ã«æ¯
æãããŠãããããã®ãããªå Žåã«ã¯ãã¬ãŒãµãŒ
ã®çµåéšåãšéé¢éšåãäºãã«åé¢ããå¿ èŠã¯ãª
ããããã«è©³ããã¯ã被æ€ç©è³ªãå«æãããŸãã¯
被æ€ç©è³ªãå«æãããšæãããæ€äœãæ¯æããã
ãã€ã³ããŒãå«ã管ãžæåã«å ããç¶ããŠäžè¿°ã
ãåã®å¢ãå ããããã®ãããªæ€å®ã«ãããŠã管
å£ã«ãããŠãã€ã³ããŒãšçµåããããã«ãªããã¬
ãŒãµãŒã¯æ€å®å¹å°ãèªç±ã«åããŠå¢ãšæ¥è§Šããã
ããªããšã¯ãªããããã«ããå¢ããã®æšèå€æŸåº
é床ã¯æ€äœå¹å°ã«ãããéé¢ãã¬ãŒãµãŒã®éã«äŸ
åããããšã«ãªãã
æãããŠãããããã®ãããªå Žåã«ã¯ãã¬ãŒãµãŒ
ã®çµåéšåãšéé¢éšåãäºãã«åé¢ããå¿ èŠã¯ãª
ããããã«è©³ããã¯ã被æ€ç©è³ªãå«æãããŸãã¯
被æ€ç©è³ªãå«æãããšæãããæ€äœãæ¯æããã
ãã€ã³ããŒãå«ã管ãžæåã«å ããç¶ããŠäžè¿°ã
ãåã®å¢ãå ããããã®ãããªæ€å®ã«ãããŠã管
å£ã«ãããŠãã€ã³ããŒãšçµåããããã«ãªããã¬
ãŒãµãŒã¯æ€å®å¹å°ãèªç±ã«åããŠå¢ãšæ¥è§Šããã
ããªããšã¯ãªããããã«ããå¢ããã®æšèå€æŸåº
é床ã¯æ€äœå¹å°ã«ãããéé¢ãã¬ãŒãµãŒã®éã«äŸ
åããããšã«ãªãã
å¥ã®å®æœæ
æ§ã«ããã°ãäžè¿°ã®åã®ãã¬ãŒãµãŒ
ã管å£ã«æ¯æããããã€ã³ããŒãšæåã«çµåãã
ãŠãããããã®ãããªå Žåã«ã¯ã被æ€ç©è³ªãå«æ
ãããŸãã¯å«æãããšæãããæ€äœãªãã³ã«äžè¿°
ã®åã®å¢ãåæã«ç®¡ãžå ããããã®ãããªå®æœæ
æ§ã«ãããŠãæ€äœäžã®è¢«æ€ç©è³ªã¯ãã¬ãŒãµãŒã«ä»£
ãã€ãŠãã€ã³ããŒãšçµåãããã€ã³ããŒããé¢ã
ããã¬ãŒãµãŒã®éãæ€äœäžã®è¢«æ€ç©è³ªéãšçŽæ¥æ¯
äŸããããã®å®æœæ æ§ã«ãããŠããã€ã³ããŒãã
é¢ãããã¬ãŒãµãŒã¯å¢ãšæ¥è§ŠããŠæ€äœå¹å°äžãžæš
èå€ãæŸåºããããšã«ãªãã
ã管å£ã«æ¯æããããã€ã³ããŒãšæåã«çµåãã
ãŠãããããã®ãããªå Žåã«ã¯ã被æ€ç©è³ªãå«æ
ãããŸãã¯å«æãããšæãããæ€äœãªãã³ã«äžè¿°
ã®åã®å¢ãåæã«ç®¡ãžå ããããã®ãããªå®æœæ
æ§ã«ãããŠãæ€äœäžã®è¢«æ€ç©è³ªã¯ãã¬ãŒãµãŒã«ä»£
ãã€ãŠãã€ã³ããŒãšçµåãããã€ã³ããŒããé¢ã
ããã¬ãŒãµãŒã®éãæ€äœäžã®è¢«æ€ç©è³ªéãšçŽæ¥æ¯
äŸããããã®å®æœæ æ§ã«ãããŠããã€ã³ããŒãã
é¢ãããã¬ãŒãµãŒã¯å¢ãšæ¥è§ŠããŠæ€äœå¹å°äžãžæš
èå€ãæŸåºããããšã«ãªãã
ãã¬ãŒãµãŒããã€ã³ããŒãšçµåãããšãã«ãã¬
ãŒãµãŒäžã«äœ¿çšãããŠãã溶解å€ãäžæŽ»åããã
ãããªå Žåã«ã¯å質æ€å®æ³ãçšããããšãå¯èœã§
ãããããªãã¡ãããšãã°ãžãŽãã·ã³ã®æ€å®ã«ã
ããŠãã€ã³ããŒãšããŠæäœãçšããã¬ãŒãµãŒã®ãª
ã¬ã³ãéšåãžçµåããé µçŽ æº¶è§£å€ãšããŠãã¹ããª
ããŒãŒãçšãããããªå Žåããã¹ããªããŒãŒã¯æ
äœãšçµåãããšãç«äœé害ãããããã«ããçµå
ãã¬ãŒãµãŒã¯å¢æº¶è§£ã®åœ¹ã«ç«ãããäžæ¹éçµåã
ã¬ãŒãµãŒã¯å¢ã溶解ããŠãã®å¢ããæ€åºå¯èœãªæš
èå€ãšæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãæŸåºããããšãã§ã
ãã
ãŒãµãŒäžã«äœ¿çšãããŠãã溶解å€ãäžæŽ»åããã
ãããªå Žåã«ã¯å質æ€å®æ³ãçšããããšãå¯èœã§
ãããããªãã¡ãããšãã°ãžãŽãã·ã³ã®æ€å®ã«ã
ããŠãã€ã³ããŒãšããŠæäœãçšããã¬ãŒãµãŒã®ãª
ã¬ã³ãéšåãžçµåããé µçŽ æº¶è§£å€ãšããŠãã¹ããª
ããŒãŒãçšãããããªå Žåããã¹ããªããŒãŒã¯æ
äœãšçµåãããšãç«äœé害ãããããã«ããçµå
ãã¬ãŒãµãŒã¯å¢æº¶è§£ã®åœ¹ã«ç«ãããäžæ¹éçµåã
ã¬ãŒãµãŒã¯å¢ã溶解ããŠãã®å¢ããæ€åºå¯èœãªæš
èå€ãšæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãæŸåºããããšãã§ã
ãã
æ¬çºæã®ä»ã®äžé¢ã«ããã°ã被æ€ç©è³ªæ€å®ãè¡
ãªãè©Šè¬ããããŸãã¯å è£ äœãæäŸãããã®ã§ã
ããããã¯ã(a)å¢æº¶è§£å€ãçµåãã被æ€ç©è³ªãŸã
ã¯ãã®é©åœãªé¡äŒŒç©ãããªããã¬ãŒãµãŒïŒããã³
(b)ãã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åºå¯èœãªæš
èå€ãšãå«ããã®ã§ãã€ãŠãåèšå¢æº¶è§£å€ãšæšè
å€ã¯åãç©è³ªã®å Žåãããå¢ããšãå«æãããã
ã¬ãŒãµãŒã®äžéšã圢æããå¢æº¶è§£å€ãªãã³ã«å¢å
éšã®å¢æº¶è§£å€ã¯ããã®ãããã«å«ãŸããå¢ã溶解
ãããã®ã§ãããè©Šè¬ããããŸãã¯å è£ äœã¯ãæ
æãŸãã¯æªæ æ圢ã®é©åœãªãã€ã³ããŒãå«ãã§ã
ãããã®ãããªãã€ã³ããŒã¯ãã¬ãŒãµãŒãšæ€å®ã
ã¹ã被æ€ç©è³ªãšã®äž¡æ¹ã«å¯Ÿãããã€ã³ããŒã§ã
ããè©Šè¬ãããã®æåã¯ãããããŸãã¯å è£ äœã«
ãããŠå¥ã ã®å®¹åšãããšãã°ã¬ã©ã¹ã³ãã«å«ãŸã
ãŠããŠãããããããããªããïŒã€ã®ã¬ã©ã¹ã³ã
ã«äžçš®ä»¥äžã®æåãéããŠããããšããããåæ§
ã«ãäžè¿°ããããã«ããã€ã³ããŒãŸãã¯ãã€ã³ã
ãŒãšãã¬ãŒãµãŒã¯åºçžæ¯æäœã®å£ã«å¡åžããŠãã
ãããããã¯ãŸãä»ã®æåããšãã°è¢«æ€ç©è³ªã®æš
æºæ¶²ïŒæ¢ç¥æ¿åºŠã®è¢«æ€ç©è³ªãæãã被æ€ç©è³ªãµã³
ãã«ïŒãå ¬ç¥ç·©è¡æ¶²çãå«ãã§ãããã
ãªãè©Šè¬ããããŸãã¯å è£ äœãæäŸãããã®ã§ã
ããããã¯ã(a)å¢æº¶è§£å€ãçµåãã被æ€ç©è³ªãŸã
ã¯ãã®é©åœãªé¡äŒŒç©ãããªããã¬ãŒãµãŒïŒããã³
(b)ãã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åºå¯èœãªæš
èå€ãšãå«ããã®ã§ãã€ãŠãåèšå¢æº¶è§£å€ãšæšè
å€ã¯åãç©è³ªã®å Žåãããå¢ããšãå«æãããã
ã¬ãŒãµãŒã®äžéšã圢æããå¢æº¶è§£å€ãªãã³ã«å¢å
éšã®å¢æº¶è§£å€ã¯ããã®ãããã«å«ãŸããå¢ã溶解
ãããã®ã§ãããè©Šè¬ããããŸãã¯å è£ äœã¯ãæ
æãŸãã¯æªæ æ圢ã®é©åœãªãã€ã³ããŒãå«ãã§ã
ãããã®ãããªãã€ã³ããŒã¯ãã¬ãŒãµãŒãšæ€å®ã
ã¹ã被æ€ç©è³ªãšã®äž¡æ¹ã«å¯Ÿãããã€ã³ããŒã§ã
ããè©Šè¬ãããã®æåã¯ãããããŸãã¯å è£ äœã«
ãããŠå¥ã ã®å®¹åšãããšãã°ã¬ã©ã¹ã³ãã«å«ãŸã
ãŠããŠãããããããããªããïŒã€ã®ã¬ã©ã¹ã³ã
ã«äžçš®ä»¥äžã®æåãéããŠããããšããããåæ§
ã«ãäžè¿°ããããã«ããã€ã³ããŒãŸãã¯ãã€ã³ã
ãŒãšãã¬ãŒãµãŒã¯åºçžæ¯æäœã®å£ã«å¡åžããŠãã
ãããããã¯ãŸãä»ã®æåããšãã°è¢«æ€ç©è³ªã®æš
æºæ¶²ïŒæ¢ç¥æ¿åºŠã®è¢«æ€ç©è³ªãæãã被æ€ç©è³ªãµã³
ãã«ïŒãå ¬ç¥ç·©è¡æ¶²çãå«ãã§ãããã
æ¬çºæã¯ãŸããæ€å®ã«çšããããå¢ã®ç¬¬äžã®éš
åã«æº¶è§£å€ïŒäžæŽ»æ§åœ¢ïŒãåãå¢ã®ç¬¬äºã®éšåã«
æ€åºå¯èœãªæšèå€ãåã段éçå¹æã«ããå¢å¹ ã
éæããããã«ããããšãé©åœã§ããããã®äºè
æäžæ§ã¯æšèå€ãšæº¶è§£å€ãšãåãå¢ã«å ¥ããšäžé©
åã§ãããããªå Žåç¹ã«çšãããããããªãã¡ã
å¢ã®å°ãªããšãäžéšã¯å¢æº¶è§£å€ãå«ã¿å¢ã®å°ãªã
ãšãäžéšã¯æ€åºå¯èœãªæšèå€ãå«ããã奜ãŸãã
å®æœæ æ§ã¯æº¶è§£å€ãšæ€åºå¯èœãªæšèå€ã®äž¡æ¹ãå«
ãå¢ã䜿çšããããšã§ããã
åã«æº¶è§£å€ïŒäžæŽ»æ§åœ¢ïŒãåãå¢ã®ç¬¬äºã®éšåã«
æ€åºå¯èœãªæšèå€ãåã段éçå¹æã«ããå¢å¹ ã
éæããããã«ããããšãé©åœã§ããããã®äºè
æäžæ§ã¯æšèå€ãšæº¶è§£å€ãšãåãå¢ã«å ¥ããšäžé©
åã§ãããããªå Žåç¹ã«çšãããããããªãã¡ã
å¢ã®å°ãªããšãäžéšã¯å¢æº¶è§£å€ãå«ã¿å¢ã®å°ãªã
ãšãäžéšã¯æ€åºå¯èœãªæšèå€ãå«ããã奜ãŸãã
å®æœæ æ§ã¯æº¶è§£å€ãšæ€åºå¯èœãªæšèå€ã®äž¡æ¹ãå«
ãå¢ã䜿çšããããšã§ããã
ãã®æ€å®æ³ã¯ãæ§ã
ãªäœæ¶²ããšãã°è¡æž
ãå°¿ç
ã«ãããŠè¢«æ€ç©è³ªã枬å®ããã®ã«çšãããããã
ã«ãããŠè¢«æ€ç©è³ªã枬å®ããã®ã«çšãããããã
æ¬çºæã¯ããã«æ¬¡ã®å®æœäŸã«é¢ããŠèšèŒããã
ããæ¬çºæã¯ããã«éå®ããããã®ã§ã¯ãªãã
ããæ¬çºæã¯ããã«éå®ããããã®ã§ã¯ãªãã
å®æœäŸ
ãã¬ãŒãµãŒã®èª¿è£œïŒãã¹ããªããŒãŒâãžãŽãã·
ã²ãã³çµåäœ ïŒâã±ããžãŽãã·ã²ãã³ã«ã«ããã·ã¡ãããã·
ã ïŒ23.06mgã0.05ããªã¢ã«ïŒãžä¹Ÿç¥DMF0.6mlã
å ããç¶ããŠïŒ®âããããã·ã¹ã¯ã·ã³ã€ãã
ïŒ5.75mgïŒã也ç¥DMFïŒ0.1mlïŒã«æº¶è§£ãã液ãã
ã³ãžã·ã¯ãããã·ã«ã«ã«ããžã€ããïŒ10.1mgïŒã
也ç¥DMFïŒ0.1mlïŒã«æº¶è§£ãã液ãå ãããäžæ©
ïŒâã§æ··åããã¬ã©ã¹ãŠãŒã«ã§éãããã®éæ
溶液ãïŒmlã®0.1Mããªã¹âHClïŒPH8.0ïŒäžã®ãã¹
ããªããŒãŒïŒ£ïŒïŒmgïŒãžïŒâã«ãŠå ãããïŒâã«
ãŠäžæ©æ··åããé å¿åé¢ã«ãããã»ãã¢ããã¯ã¹
â25ã«ã©ã ã«éæäžæŸæ¶²ãéããçµåäœãåã
ç·©è¡æ¶²ã§æº¶åºããã
ã²ãã³çµåäœ ïŒâã±ããžãŽãã·ã²ãã³ã«ã«ããã·ã¡ãããã·
ã ïŒ23.06mgã0.05ããªã¢ã«ïŒãžä¹Ÿç¥DMF0.6mlã
å ããç¶ããŠïŒ®âããããã·ã¹ã¯ã·ã³ã€ãã
ïŒ5.75mgïŒã也ç¥DMFïŒ0.1mlïŒã«æº¶è§£ãã液ãã
ã³ãžã·ã¯ãããã·ã«ã«ã«ããžã€ããïŒ10.1mgïŒã
也ç¥DMFïŒ0.1mlïŒã«æº¶è§£ãã液ãå ãããäžæ©
ïŒâã§æ··åããã¬ã©ã¹ãŠãŒã«ã§éãããã®éæ
溶液ãïŒmlã®0.1Mããªã¹âHClïŒPH8.0ïŒäžã®ãã¹
ããªããŒãŒïŒ£ïŒïŒmgïŒãžïŒâã«ãŠå ãããïŒâã«
ãŠäžæ©æ··åããé å¿åé¢ã«ãããã»ãã¢ããã¯ã¹
â25ã«ã©ã ã«éæäžæŸæ¶²ãéããçµåäœãåã
ç·©è¡æ¶²ã§æº¶åºããã
å®æœäŸ
äžæŽ»æ§ç¶æ
ãã¹ããªããŒãŒãšæšèå€ãå
å
ãã
ããªããœãŒã ã®èª¿è£œ 100mlãã©ã¹ã³ãžãã³ã¬ã¹ãããŒã«ïŒ48mgïŒããž
ã¹ãã¢ãã€ã«ãã¹ãã¢ããžã«ã³ãªã³ïŒ104mgïŒã
ãã³ãžã€ãœãããã«ãšãŒãã«ïŒ6.0mlïŒãšã¡ã¿ã
ãŒã«ïŒ1.0mlïŒã®æ··åç©ãå ããããã®æº¶æ¶²ãå
転èžçºåšã§èžçºãããé«æžå§èžçºã«ããæåŸã®ã
ãè·¡éã®ææ©æº¶åªãé€ããEDTAïŒ0.01MïŒãšïŒ
âã¡ãã«ãŠã³ããªããšãªã«ãã¹ããšãŒã
ïŒ0.05MïŒãå«ã0.01Mãªã³é žç·©è¡æ¶²ïŒPH7.5ïŒïŒ
mläžã«ãã¹ããªããŒãŒïŒ£ïŒïŒmgïŒã溶解ãã液ã
å ããã枊巻ç¶ã«æ··ããŠè質ãåæ£ããã次ãã§
宀枩ã§ïŒåéè¶ é³æ³¢åŠçãããè£ å ¥ããããªããœ
ãŒã ããè¶ é å¿åé¢ïŒ75000ïœã§30åéãïŒåïŒ
ã«ããæ®åé µçŽ ãšæ®åæšèå€ããåé¢ããã
ããªããœãŒã ã®èª¿è£œ 100mlãã©ã¹ã³ãžãã³ã¬ã¹ãããŒã«ïŒ48mgïŒããž
ã¹ãã¢ãã€ã«ãã¹ãã¢ããžã«ã³ãªã³ïŒ104mgïŒã
ãã³ãžã€ãœãããã«ãšãŒãã«ïŒ6.0mlïŒãšã¡ã¿ã
ãŒã«ïŒ1.0mlïŒã®æ··åç©ãå ããããã®æº¶æ¶²ãå
転èžçºåšã§èžçºãããé«æžå§èžçºã«ããæåŸã®ã
ãè·¡éã®ææ©æº¶åªãé€ããEDTAïŒ0.01MïŒãšïŒ
âã¡ãã«ãŠã³ããªããšãªã«ãã¹ããšãŒã
ïŒ0.05MïŒãå«ã0.01Mãªã³é žç·©è¡æ¶²ïŒPH7.5ïŒïŒ
mläžã«ãã¹ããªããŒãŒïŒ£ïŒïŒmgïŒã溶解ãã液ã
å ããã枊巻ç¶ã«æ··ããŠè質ãåæ£ããã次ãã§
宀枩ã§ïŒåéè¶ é³æ³¢åŠçãããè£ å ¥ããããªããœ
ãŒã ããè¶ é å¿åé¢ïŒ75000ïœã§30åéãïŒåïŒ
ã«ããæ®åé µçŽ ãšæ®åæšèå€ããåé¢ããã
å®æœäŸ
æ€å®æ¹æ³
 æãžãŽãã·ã³è¢«èŠè©Šéšç®¡ãžæ¬¡ã®ãã®ãå ã
ãïŒ ïŒ ãžãŽãã·ã³æšæºæ¶²ãŸãã¯è¡æž ãµã³ãã«ã
50ÎŒ ïŒ ãã¬ãŒãµãŒïŒãžãŽãã·ã³âãã¹ããªããŒãŒ
çµåäœã100ÎŒãç·©è¡æ¶²ïŒ0.01Mãã¹ããšãŒ
ããPH7.5ã0.15M KClããã³ïŒmgïŒml
BSAïŒã«ãã®çµåäœã溶ãããæ¿åºŠãçŽ0.1
mgïŒmlã«èª¿æŽããã
ãïŒ ïŒ ãžãŽãã·ã³æšæºæ¶²ãŸãã¯è¡æž ãµã³ãã«ã
50ÎŒ ïŒ ãã¬ãŒãµãŒïŒãžãŽãã·ã³âãã¹ããªããŒãŒ
çµåäœã100ÎŒãç·©è¡æ¶²ïŒ0.01Mãã¹ããšãŒ
ããPH7.5ã0.15M KClããã³ïŒmgïŒml
BSAïŒã«ãã®çµåäœã溶ãããæ¿åºŠãçŽ0.1
mgïŒmlã«èª¿æŽããã
 宀枩ã§10ã30åéã€ã³ããŠããŒãããã
 ãã«ã³ãããç·©è¡æ¶²ïŒmlãå ãããã«ã³ãã
ãã
ãã
 åºè³ªãå ããããªããœãŒã ãããªã¹âHClç·©
è¡æ¶²ïŒããªã¹âHCl 50ïœïŒãå¡©åäºéïŒïœïŒã
å¡©åã«ã«ã·ãŠã ïŒïœïŒãBSA15mgïŒmlãPHïŒ
7.3ïŒäžã§æžæ¿åããããïŒã10åéã€ã³ããŠ
ããŒããããPHïŒã«èª¿æŽããã0.5M EDTA1
mlãå ãããè¢å 床ãèªã¿åãïŒçŽ410nïœã§
å±èµ·ã450nïœã§è¢å çºå ïŒã
è¡æ¶²ïŒããªã¹âHCl 50ïœïŒãå¡©åäºéïŒïœïŒã
å¡©åã«ã«ã·ãŠã ïŒïœïŒãBSA15mgïŒmlãPHïŒ
7.3ïŒäžã§æžæ¿åããããïŒã10åéã€ã³ããŠ
ããŒããããPHïŒã«èª¿æŽããã0.5M EDTA1
mlãå ãããè¢å 床ãèªã¿åãïŒçŽ410nïœã§
å±èµ·ã450nïœã§è¢å çºå ïŒã
æ¬çºæã¯æ€å®ã®æ床ãåäžããç¹ã§ç¹ã«æå©ã§
ãããåå¿ãçããä¿¡å·ã®é£éçæ§è³ªã¯é«ãå¢å¹
ããããããããã«ããæ¬çºææ€å®æ³ã¯åŸæ¥æè¡
ã®éæŸå°æ§æ€å®æ³ããé«æ床ã§ããã
ãããåå¿ãçããä¿¡å·ã®é£éçæ§è³ªã¯é«ãå¢å¹
ããããããããã«ããæ¬çºææ€å®æ³ã¯åŸæ¥æè¡
ã®éæŸå°æ§æ€å®æ³ããé«æ床ã§ããã
æ¬çºæã®æ§ã
ãªæ¹è¯ããã³å€åœ¢ã¯äžè¿°ã®èšèŒã
ãç §ãããŠã¿ãã°å¯èœã§ãããããããç¹èš±è«æ±
ã®ç¯å²å ã§ãæ¬çºæã¯ç¹ã«èšèŒãããã®ã«ã¯éå®
ãããªãã
ãç §ãããŠã¿ãã°å¯èœã§ãããããããç¹èš±è«æ±
ã®ç¯å²å ã§ãæ¬çºæã¯ç¹ã«èšèŒãããã®ã«ã¯éå®
ãããªãã
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ ãã¬ãŒãµãŒãšè¢«æ€ç©è³ªããå°ãªããšã被æ€ç©
質ã«ç¹ç°çã«çµåãããã€ã³ããŒãšæ¥è§Šãããã
ã®éåèšãã¬ãŒãµãŒã¯å¢æº¶è§£å€ãšçµåãããªã¬ã³
ããããªããåèšãã¬ãŒãµãŒã®åèšãªã¬ã³ãéšå
ã¯è¢«æ€ç©è³ªãšãã€ã³ããŒã®äžã€ã«çµåããããã«
ããåèšãã¬ãŒãµãŒã®äžéšããã€ã³ããŒã«çµå
ãïŒ éçµåãã¬ãŒãµãŒãå¢ãšæ¥è§Šããããã®éåèš
å¢ã®å°ãªããšãäžéšã¯ãã®å éšã«æ€åºå¯èœãªæšè
å€ãå«ã¿ãåèšå¢ã®å°ãªããšãäžéšã¯äžæŽ»æ§åœ¢ã®
å¢æº¶è§£å€ã§ãã€ãŠå¢ããæŸåºããããšæŽ»æ§ã«ãªã
ãã®ãå«ã¿ïŒ åèšå¢ããæŸåºãããæšèå€ã被æ€ç©è³ªã®éãš
ããŠæž¬å®ããããšãããªã被æ€ç©è³ªã®æ€å®æ³ã ïŒ å¢ãæ€åºå¯èœãªæšèå€ãšæº¶è§£å€ã®äž¡æ¹ãå«ã
ç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ€å®æ³ã ïŒ å¢ããªããœãŒã ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé
èšèŒã®æ€å®æ³ã ïŒ æº¶è§£å€ãé µçŽ ã§ãããå¢å éšã«ãããåèšé µ
çŽ ãäžæŽ»æ§åœ¢ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®
æ€å®æ³ã ïŒ ãã€ã³ããŒãåºçžæ¯æäœã«æ¯æãããŠããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ€å®æ³ã ïŒ ãã€ã³ããŒã被æ€ç©è³ªãšãã¬ãŒãµãŒã®äž¡æ¹ã«
察ãããã€ã³ããŒã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èš
èŒã®æ€å®æ³ã ïŒ çµåããããã³æªçµåãã¬ãŒãµãŒéšåãæšè
å€æŸåºåã«åé¢ãããæ€å®æ³ãå質æ€å®æ³
ïŒhomogeneous assayïŒã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬
ïŒé èšèŒã®æ€å®æ³ã ïŒ æ€åºå¯èœãªæšèå€ãéæŸå°æ§æšèå€ã§ããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ€å®æ³ã ïŒ è¢«æ€ç©è³ªããããã³ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬
ïŒé èšèŒã®æ€å®æ³ã ïŒïŒ 被æ€ç©è³ªãäœæ¶²æ€äœäžã«ååšãããã®ã§ã
ãç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒé èšèŒã®æ€å®æ³ã ïŒïŒ ãã¬ãŒãµãŒãšè¢«æ€ç©è³ªãå°ãªããšã被æ€ç©
質ã«ç¹ç°çã«çµåãããã€ã³ããŒãšæ¥è§Šãããã
ã®éåèšãã¬ãŒãµãŒã¯é µçŽ ãããªã掻æ§ãªããœãŒ
ã 溶解å€ãšçµåãããªã¬ã³ããããªããåèšãã¬
ãŒãµãŒã®ãªã¬ã³ãéšåã¯ãã€ã³ããŒãšè¢«æ€ç©è³ªã®
äžã€ã«çµåããããã«ãããã¬ãŒãµãŒã®äžéšãã
ã€ã³ããŒã«çµåãïŒ éçµåãã¬ãŒãµãŒãããã®å éšã«éæŸå°æ§ã®æ€
åºå¯èœãªæšèå€ããã³äžæŽ»æ§åœ¢ã®é µçŽ ãããªããª
ããœãŒã 溶解å€ãå«æãããªããœãŒã ãšæ¥è§Šã
ãããã®éãªããœãŒã ãšã®æ¥è§Šã¯ãªããœãŒã å ã®
ãªããœãŒã 溶解å€ã«å¯Ÿãã賊掻å€ã®ååšäžã«è¡ã
ããããã«ããæ€åºå¯èœãªæšèå€ã溶解ãããªã
ãœãŒã ããæŸåºããããªããœãŒã ããæŸåºããã
ãªããœãŒã 溶解å€ãããã«å¥ã®ãªããœãŒã ã溶解
ããããã®æŽ»æ§ãæããŠé£éçå¹æãããããïŒ æŸåºãããæšèå€ã被æ€ç©è³ªã®éãšããŠæž¬å®ã
ãïŒ ããšãããªãé µçŽ ã®æ€åºæ³ã§ããç¹èš±è«æ±ã®ç¯å²
第ïŒé èšèŒã®æ€å®æ³ã ïŒïŒ ãªããœãŒã å éšã«ã«ã«ã·ãŠã äŸåæ§é µçŽ 溶
解å€ãšã«ã«ã·ãŠã é¯åå€ãšãå«ãŸããŠãªããœãŒã
å éšã§ã¯é µçŽ 溶解å€ãäžæŽ»åãã賊掻å€ãæŸåºã
ãã溶解å€ã掻æ§åããã®ã«ååéã®ã«ã«ã·ãŠã
ãå«ãã§ãªãç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®æ€å®
æ³ã ïŒïŒ ãªããœãŒã å éšã«ã¢ãé µçŽ ãå«ãŸãã賊掻
å€ãè£é µçŽ ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®
æ€å®æ³ã ïŒïŒ ãã€ã³ããŒãåºçžæ¯æäœã«æ¯æãããŠãã
ç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®æ€å®æ³ã ïŒïŒ 被æ€ç©è³ªãäœæ¶²äžã§æž¬å®ãããç¹èš±è«æ±ã®
ç¯å²ç¬¬ïŒïŒé èšèŒã®æ€å®æ³ã ïŒïŒ çµåããã³æªçµåãã¬ãŒãµãŒéšåãæšèå€
æŸåºåã«åé¢ãããæ€å®æ³ãå質æ€å®æ³ã§ããç¹
èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®æ€å®æ³ã ïŒïŒ 被æ€ç©è³ªããããã³ã§ããç¹èš±è«æ±ã®ç¯å²
第ïŒïŒé èšèŒã®æ€å®æ³ã ïŒïŒ (a)å¢æº¶è§£å€ãšçµåãããªã¬ã³ããããªãã
ã¬ãŒãµãŒãšã(b)å°ãªããšã被æ€ç©è³ªã«ç¹ç°çã«çµ
åãããã€ã³ããŒãšã(c)å¢ã§ãã€ãŠã該å¢ã®å°ãª
ããšãäžéšããã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãå«
ã¿ãã€åèšå¢ã®å°ãªããšãäžéšããã®å éšã«æ€åº
å¯èœãªæšèå€ãå«ããã®ãå å«ããçµåããããª
ããåèšãã¬ãŒãµãŒã®ãªã¬ã³ãéšåã¯è¢«æ€ç©è³ªãš
ãã€ã³ããŒã®äžã€ã«çµåããããã«ããåèšãã¬
ãŒãµãŒã®äžéšããã€ã³ããŒã«çµåããããšãç¹åŸŽ
ãšãã被æ€ç©è³ªæ€å®çšè©Šè¬ãããã ïŒïŒ å¢ããã®å éšã«äžæŽ»æ§åœ¢ã®å¢æº¶è§£å€ãšæ€åº
å¯èœãªæšèå€ã®äž¡æ¹ãå«ã¿ãåèšæ€åºå¯èœãªæšè
å€ãéæŸå°æ§æšèå€ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒ
é èšèŒã®ãããã ïŒïŒ å¢ããªããœãŒã ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒ
ïŒé èšèŒã®ãããã ïŒïŒ 溶解å€ãé µçŽ ã§ãããå¢ã®å éšã«ããé µçŽ
ãäžæŽ»æ§åœ¢ã§ããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®
ãããã ïŒïŒ ãªããœãŒã äžã®é µçŽ 溶解å€ãã«ã«ã·ãŠã äŸ
åæ§é µçŽ ã§ããããªããœãŒã ã®å éšã«ã¯ã«ã«ã·ãŠ
ã é¯åå€ãããã«å«æããé µçŽ ãäžæŽ»æ§åããŠã
ãç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒïŒé èšèŒã®ãããã ïŒïŒ çµåããããã«è¢«æ€ç©è³ªçšãã€ã³ããŒãšã
被æ€ç©è³ªã®æšæºæ¶²ãšãå¢ã®å éšã«ããå¢æº¶è§£å€çš
ã®æŽ»æ§åç·©è¡æ¶²ãšãå«æããç¹èš±è«æ±ã®ç¯å²ç¬¬ïŒ
ïŒé èšèŒã®ãããã
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US643478 | 1984-08-23 | ||
US06/643,478 US4666830A (en) | 1984-08-23 | 1984-08-23 | Assay employing sacs and sac lysing agent |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS6171362A JPS6171362A (ja) | 1986-04-12 |
JPH0467916B2 true JPH0467916B2 (ja) | 1992-10-29 |
Family
ID=24580991
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP60185612A Granted JPS6171362A (ja) | 1984-08-23 | 1985-08-23 | ãªã¬ã³ãã®å¢æåææ³ |
Country Status (8)
Country | Link |
---|---|
US (1) | US4666830A (ja) |
EP (1) | EP0174753B1 (ja) |
JP (1) | JPS6171362A (ja) |
AU (1) | AU582775B2 (ja) |
DE (1) | DE3569297D1 (ja) |
MY (1) | MY100369A (ja) |
NZ (1) | NZ212778A (ja) |
ZA (1) | ZA855585B (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4828982A (en) * | 1985-05-28 | 1989-05-09 | Becton, Dickinson & Company | Vesticles and use thereof in an enzyme assay |
US4752572A (en) * | 1985-08-30 | 1988-06-21 | Eastman Kodak Company | Lipid vesicles containing labeled species and their analytical uses |
US4874710A (en) * | 1986-02-20 | 1989-10-17 | Becton Dickinson And Company | Assay and product in which binder and liposomes are supported on a solid support |
EP0274343A1 (en) * | 1987-01-06 | 1988-07-13 | IntraCel Corporation | A system of reactants involving an apo-enzyme useful in immunological analysis and a method for carrying out immunoassays with this system |
EP0300682A1 (en) * | 1987-07-13 | 1989-01-25 | The University Of Tennessee Research Corporation | A homogeneous, lipsome-based signal amplification method for assays involving enzymes |
DE3836656A1 (de) * | 1988-10-27 | 1990-05-03 | Boehringer Mannheim Gmbh | Neue digoxigenin-derivate und ihre verwendung |
US5143825A (en) * | 1990-03-01 | 1992-09-01 | Abbott Laboratories | Stabilized substrate for use in an immunoassay |
US6927025B1 (en) * | 1997-09-03 | 2005-08-09 | Biovation Limited | Methods for protein screening |
US6306642B1 (en) * | 1997-11-24 | 2001-10-23 | Quidel Corporation | Enzyme substrate delivery and product registration in one step enzyme immunoassays |
US11541105B2 (en) | 2018-06-01 | 2023-01-03 | The Research Foundation For The State University Of New York | Compositions and methods for disrupting biofilm formation and maintenance |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4235792A (en) * | 1977-04-14 | 1980-11-25 | The Governing Council Of The University Of Toronto | Immunological materials |
US4193983A (en) * | 1978-05-16 | 1980-03-18 | Syva Company | Labeled liposome particle compositions and immunoassays therewith |
JPS56500030A (ja) * | 1979-01-18 | 1981-01-08 | ||
US4342826A (en) * | 1980-02-04 | 1982-08-03 | Collaborative Research, Inc. | Immunoassay products and methods |
US4483921A (en) * | 1981-01-12 | 1984-11-20 | Collaborative Research, Inc. | Immunoassay with antigen or antibody labeled liposomes sequestering enzyme |
AU532728B2 (en) * | 1981-11-05 | 1983-10-13 | Miles Laboratories Inc. | Enzyme occluded in solvent pockets of a membrane |
US4483929A (en) * | 1982-05-03 | 1984-11-20 | Liposome Technology Incorporated | Liposomes with glycolipid-linked antibodies |
US4517303A (en) * | 1982-10-20 | 1985-05-14 | E. I. Du Pont De Nemours And Company | Specific binding assays utilizing analyte-cytolysin conjugates |
IL72269A0 (en) * | 1983-11-30 | 1984-10-31 | Miles Lab | Specific binding assay methods and reagent systems |
-
1984
- 1984-08-23 US US06/643,478 patent/US4666830A/en not_active Expired - Fee Related
-
1985
- 1985-07-18 NZ NZ212778A patent/NZ212778A/xx unknown
- 1985-07-24 ZA ZA855585A patent/ZA855585B/xx unknown
- 1985-07-30 AU AU45608/85A patent/AU582775B2/en not_active Ceased
- 1985-08-16 EP EP85305865A patent/EP0174753B1/en not_active Expired
- 1985-08-16 DE DE8585305865T patent/DE3569297D1/de not_active Expired
- 1985-08-23 JP JP60185612A patent/JPS6171362A/ja active Granted
-
1987
- 1987-09-29 MY MYPI87002136A patent/MY100369A/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU582775B2 (en) | 1989-04-13 |
AU4560885A (en) | 1986-02-27 |
ZA855585B (en) | 1986-03-26 |
EP0174753A1 (en) | 1986-03-19 |
US4666830A (en) | 1987-05-19 |
MY100369A (en) | 1990-08-28 |
DE3569297D1 (en) | 1989-05-11 |
EP0174753B1 (en) | 1989-04-05 |
NZ212778A (en) | 1989-01-27 |
JPS6171362A (ja) | 1986-04-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4708933A (en) | Immunoliposome assay-methods and products | |
US4904583A (en) | Cascade immunoassay by multiple binding reactions | |
US4483929A (en) | Liposomes with glycolipid-linked antibodies | |
US4978625A (en) | Fluorescence immunoassay using water insoluble dyes | |
US7635571B2 (en) | Amplified signal in binding assays | |
AU551950B2 (en) | Immunoassay products and methods | |
US4622294A (en) | Liposome immunoassay reagent and method | |
CA1111763A (en) | Lipophilic particles non-covalently bonded to label and ligand for binding assay | |
US4703017A (en) | Solid phase assay with visual readout | |
US4695554A (en) | Sac or liposome containing dye (sulforhodamine) for immunoassay | |
US4874710A (en) | Assay and product in which binder and liposomes are supported on a solid support | |
US4855240A (en) | Solid phase assay employing capillary flow | |
US4891324A (en) | Particle with luminescer for assays | |
WO1982002772A1 (en) | Microencapsulated fluorescer composition,test kit and system for immunoassay | |
US4713324A (en) | Inverted latency specific binding assay | |
US4656129A (en) | Assay for a ligand by use of supported binder and sac lysing agent | |
JPH0467916B2 (ja) | ||
US4888288A (en) | Vesicles resistant to enzyme lysis and use thereof in an enzyme assay | |
US4828982A (en) | Vesticles and use thereof in an enzyme assay | |
Orellana et al. | Europium chelate-loaded liposomes: a tool for the study of binding and integrity of liposomes | |
US4940669A (en) | Sac including a detectable metal marker and use thereof in an assay | |
JPH01500848A (ja) | å ç«ãªããœâã å®éæ³âæ¹æ³ãšææ | |
JPS62163966A (ja) | è£äœäŸ¡æž¬å®çšè©Šè¬ | |
JPS63158461A (ja) | å ç«åæçšè©Šè¬ | |
JPH04122859A (ja) | å ç«åæçšè©Šè¬ |