JPH0460180A - Pump and reverse flow preventing device - Google Patents

Abstract

PURPOSE:To make a liquid feed possible with no damage of a flotage by forming a reverse flow-preventing valve, connecting a flexible tube on the halfway of a flow path inserted in a housing, and a feed liquid part between a plurality of housings and utilizing expansion and contraction of the tube so as to feed cell floating liquid or the like. CONSTITUTION:Pipes 11 to 41 are provided in four housings 10 to 40, and air bleed valves 13 to 43 are provided in an upper part of each housing 10 to 40 while connecting the pipes by flexible plastic tubes 12, 32, 42. A volume adjusting bellows 22 is provided in an upper part of the housing 20, and one end of this bellows 22 is opened to the outside. Further, pipes 15, 25 and 35, 45 in a lower part of each housing 10 to 40 are connected by pipes 50, 51 respectively to a pump 4 which is a pressure increasing/reducing mechanism. A part of the housing 40 and a part of the housing 10 respectively serve as a pump suction port and a pump delivery port, and a pump main unit is contracted by closing the delivery port, when the pump main unit is expanded by opening the suction port, and by closing the suction port when the delivery port is opened. A culture solution is taken in from the suction port and fed out from the delivery port by action thus obtained.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to a pump and a backflow prevention device, and in particular, to a pump and a backflow prevention device for transferring a liquid in which cultured animal/plant cells, microalgae, etc. are suspended without destroying the suspended matter. The present invention relates to a pump for circulating or circulating water, and a backflow prevention device.

Conventional technology Conventionally, turbine pumps, bellows pumps, tube pumps, screw pumps, etc. have been commonly used to transfer or circulate liquids containing cultured animal and plant cells, microalgae such as chlorella, etc. It was a target.

Problems to be Solved by the Invention According to the above-mentioned conventional method, it can be applied without particular problems to liquids that do not contain floating substances or liquids that contain floating substances that cannot be destroyed by mechanical impact. However, in the technical field of cell culture of animals, plants, and animals that has become widely practiced in recent years, these cultured materials are extremely vulnerable to mechanical shock, so rotation and sliding, which are commonly used in the past, are not suitable. Another problem is that it is impossible to use a pump whose principle is to pump liquid by squeezing the tube.

Furthermore, among conventionally used pumps, bellows pumps and piston pumps, which utilize the expansion and contraction of a bellows driven by a motor or the reciprocating motion of a piston, have relatively mild mechanical shocks. It is considered. However, metal or resin check valves are used to regulate the direction of fluid flow, and as a result, cultured cells, etc., which are susceptible to mechanical shock as mentioned above, are rarely destroyed by the check valves. There is a problem in that the contents of the destroyed cells cannot avoid the physiological adverse effects that they have on the remaining cells, and at present no pump or backflow prevention device that can satisfy these conditions has been developed. be.

The present invention was proposed in view of the above-mentioned circumstances and to solve the problems. And, the purpose of the present invention is to
A pump and a pump capable of transporting or circulating a liquid containing suspended substances that are easily destroyed by mechanical shock, such as cultured animal/plant cells or microalgae, at any speed without damaging these suspended substances. An object of the present invention is to provide a backflow prevention device with low mechanical impact.

Means for Solving the Problems The present invention employs the following means to solve the above problems.

That is, it includes a suction port housing and a discharge port housing equipped with a backflow prevention mechanism, and a liquid delivery section housing, and includes water,
A plurality of housings are provided which are filled with liquid such as oil and become airtight, and a flow path passing through the housing is formed between the plurality of housings, and a flexible tube is connected in the middle of this flow path. A non-return valve and a liquid feeding section are formed, and cells are transported by using the expansion and contraction of the tube induced by alternately repeating pressurization and depressurization by the pressurization and depressurization mechanism connected between each S/S container. The pump is characterized by pumping floating liquid, etc.

It also has two housings that serve as an inlet and an outlet and are filled with liquid such as water and oil, and within this housing, a check valve with a flexible tube connected to the inlet and outlet is installed. A backflow prevention device characterized in that the flow path is opened and closed by utilizing expansion and contraction of the tube induced by alternately repeating pressurization and depressurization by a pressurization and depressurization mechanism connected to the housing. It was used as a device.

A liquid such as water filled in the working housing is moved between the suction port housing, the liquid sending unit housing, and the discharge port housing in a closed system, and when one is pressurized, the other is set to a reduced pressure state. The cell suspension is transferred by alternately repeating expansion and contraction of the tube within the housing induced by this pressurization and depressurization.

Also, backflow prevention devices move liquid alternately between the inlet housing and the outlet housing, apply pressure or depressurization, and expand or contract the tubes in the inlet housing and the outlet housing. Since it regulates the direction of fluid flow, it can be used in conjunction with conventional pumps to
Cultured plant cells, etc. can be transferred more gently.

EXAMPLE Hereinafter, an example of the present invention will be described in detail based on the drawings.

FIG. 1 shows a pump and backflow prevention device according to the invention. According to the figure, four housings 10,
20, 30, and 40 are removably connected to each other by a flange connection (not shown) or the like. These housings 10, 20, 30° 40 are preferably made of metal or pressure-resistant plastic.

The four housings 10. 20. 30. Inside 40 are metal or plastic vibrators 11°21.
31.41, each housing 10°20.30
．． 40.

That is, the pipe 11 extends from the housing 10 to the outside, and the pipe 21 extends from the inside of the housing 10 to the housing 2.
0 and extends into the /S housing 30. In addition, the pipe 31 is connected to the nozzle 30 and the housing 4.
0, and the pipe 41 is connected to the housing 40.
Extended to the outside.

These vibrators are then connected with a flexible plastic tube. That is, no-usage leg 10
The pipe 11 and the sleeve 21 are the plastic tube 12 inside, and the pipe 2 is the plastic tube 12 inside the housing 30.
1 and 31 are tubes 32, and inside the housing 40, the pipes 31 and 41 are connected by tubes 42, respectively.

For the plastic tubes 12, 32, and 42, the tube ends are placed over each pipe end, and the covered portion is fitted with an O-ring I.
6 and a stopper 17, and is tightly connected to each pipe.

A liquid flow path 3 is formed by each of these pipes 1.1.21.31.41 and each tube 12, 32.42.

In addition, the plastic tube 12. 32. The material 42 is preferably thin and made of a flexible material. Examples include those made of fluororesin, polyethylene, polyethylene, rubber, etc. with a wall thickness of 30 to 100 microns.

At the top of each housing 10.20, 30.40, an air vent valve 13, 23, 33.43 is provided.

The air bleed valves 13, 23, 33, 43 are not only used to bleed the nozzle but also serve as an inlet for water or other liquids to be supplied into the housing.

Furthermore, a bellows 22 for capacity adjustment is provided on the upper part of the housing 20. One end of this bellows 22 is opened to the outside.

The capacitance between the bellows 22 and each of the plastic tubes 112, 32, 42 has the following relationship.

That is, now the capacity of the plastic tube 12, 32° 42 provided in the housing 10.30.40 is
Let t'LVA, VC, VD and L respectively, and the capacity of the bellows provided in the housing 20 be VB. At this time, the capacitance of each part is determined so that the relationship between them is VA+VB≧VC+VD.

Pipes 15, 25, 35, 45 are provided at the bottom of each housing 10, 20, 30, 40, respectively. The pipes 15 and 25 are connected to the pipes 35 and 45 by the pipe 50.
and are connected by piping 51 to the pump 4, which is a pressure increase/reduction mechanism.

This pump 4 is not particularly limited as long as it can reverse the liquid feeding direction.

Four housings with this structure 10°20.3
Of the 0.40, 30 parts of the housing is the liquid feeding book body,
Housing sections 10 and 40 are check valves and housing section 20 is a reservoir.

In addition, the housing 40 is the pump inlet, and the housing 1 is the pump inlet.
0 part is the pump discharge port, and this housing 40.10
The backflow prevention device 2, which is the second invention of the present invention, is constituted by the plastic tube 12° 42, and the pump 4 of the pressurization/decompression mechanism as the main elements.

The pump 1 of the first invention includes the backflow prevention device 2, the housing 2030, the plastic tube 32, the pump 4 of the pressurization/decompression mechanism, and the like.

Next, the operation of this embodiment having such a configuration will be explained.

In this embodiment, opening and closing of the valve and expansion and contraction of the pump body are used to circulate the culture solution.

That is, when the suction port valve is open and the pump body is expanding, the discharge port valve is closed, and when the discharge port valve is open, the suction port valve is closed and the pump body is contracted. Through such expansion/contraction and opening/closing, the culture solution is taken into the pump body from the suction port and sent out from the discharge port. By repeating this, the culture solution is transferred in one direction. In order to perform such expansion/contraction and opening/closing as a series of operations, in this embodiment, the liquid filled in the housing is
It is moved alternately between the suction section, the pump body, and the discharge section.

Next, the operation of the pump will be explained based on FIGS. 3(a) and 3(b).

A liquid such as water is injected into each housing 10, 20, 30.40 from the respective air vent valve 13, 23, 33.43 to fill each housing 10°20, 30.40. After that, air vent valve 13, 23, 33.43
is closed to bring each housing 10.20, 30.40 into a sealed state.

The housing 30 is operated by operating the pump 4 of the pressurization/decompression mechanism.
In the housing 10 in which the liquid in the housing 10 and 40 is moved to the housings 10 and 20, the plastic tube 12 in the housing 10 is pressurized by the sent liquid and contracts, thereby blocking the flow path 3. At this time, by making the diameter of the pipe 15 larger than that of the pipe 25, the speed at which the liquid flows into the housing 10 can be made higher than the speed at which the liquid flows into the housing 20, and shrinkage of the plastic tube 12 can be prioritized.

On the other hand, within the housing 40, as a result of the decrease in liquid, the tube 42 expands and the flow path 3 opens. At the same time, the tube 32 inside the housing 30 also expands, and this expansion causes the flow path 3
The cell suspension sent into the tube 32 is sucked into the tube 32 (FIG. 3 (a)). At this time, by making the diameter of the pipe 45 larger than that of the pipe 35, the housing 4
The outflow speed from 0 becomes faster, and tube 42 expands before tube 32.

At this time, the bellows 22 is contracted within the housing 20. As mentioned above, the capacity of the tube 12° bellows 22 and the capacity of the tube 32.42 are balanced. Thus, the amount of liquid required to fully expand the tube 32.42 can be pumped from within the housing 30.40 into the housing 10.20, and the tube 12 is then completely deflated.

Next, the liquid feeding is reversed by rotating the pump 4 of the pressurization/depressurization mechanism or by switching the solenoid valve.

The tube 42 within the housing 40 is connected to the housing 10.
It is contracted by the liquid sent from 20, and as a result, the flow path 3 is blocked.

On the other hand, inside the housing 10, as a result of the decrease in liquid, the tube 12 expands and the flow path 3 is opened.

At the same time, the cell suspension is sent out due to the contraction within the housing 30 (FIG. 3 (b)). At this time, pipes 15 and 4
5 has a larger diameter than the pipes 25 and 35, so the inflow and outflow speeds are faster, and as a result, the tube 42 contracts faster than the tube 32, and the tube I2 expands faster than the bellows 22.

Four such housings10. 20. 30°4
By repeating the process of expanding and contracting the tube 12, 32, 42 and the bellows 22, the cell suspension is continuously transferred.

In addition, in this pump, the pump 4 of the pressurization/decompression mechanism is
There is no particular limitation as long as the direction of liquid feeding can be reversed. That is, a device whose rotating direction is reversed may be used, or, as shown in FIG. 2, the liquid feeding direction may be reversed by combining a pump and a solenoid valve that rotate in the same direction.

The principle of liquid feeding by the gear pump 46 and the electromagnetic valves 47A to 47D will be described based on FIG. 2(a), port C).

When the pump 46 is operated with the electromagnetic valves 47A and 47D closed and 47B and 47C open, the liquid flows through the valves 47B and 4.
7C and flows in the direction of arrow A shown in FIG. 2(A).

Next, when the solenoid valves are switched to close the valves 47B and 47C and open the valves 47A and 47D, the liquid flows through the valves 47A and 47D.
7D, and begins to flow in the direction of arrow B in FIG. 2 (b).

Note that the transfer rate of fluid such as cell suspension by the pump of the present invention can be set to any value by setting the capacity of the tube in the housing, the flow rate of liquid by the pump of the pressurization/decompression mechanism, and the reversal interval of the flow direction. It is possible to choose.

In addition, in this pump, fluid is intermittently sucked in and discharged, resulting in so-called pulsating flow, but if pulsating flow is undesirable, two pumps can be placed in parallel and operated in opposite phases. This makes it possible to prevent pulsating flow. According to this example of a real gallery, the following effects are produced.

That is, in order to transfer the cell suspension, the liquid in the housing 10, 20, 30, 40 is transferred to the housing 1020.
and the housing 30, 40 to repeat suction and discharge. In order to move the liquid like a seesaw, a pump 4 such as a gear pump operated by a solenoid valve that can reverse the liquid feeding direction is used. Therefore, the entire device is simple and can be downsized.

In addition, as a result of the rapid movement of liquid by the operation of the pump 4 of the pressurization/decompression mechanism, the tube is expanded and contracted quickly. In this way, the response was faster and the cell suspension transfer function was improved.

In addition, plastic tubes that repeatedly expand and contract,
It is made of thin and flexible material. Therefore,
The tube easily expands and contracts even with slight pressure, and pumps out the liquid that was filled inside, containing suspended cultured animal and plant cells and microalgae that are vulnerable to mechanical shock. At this time, even if a part of the cell was caught between the thin tube membranes, it was no longer destroyed.

Next, a second embodiment will be described based on FIG.

According to this embodiment, the two housings 60 and 70 are removably connected to each other. These housings 60,70 are preferably made of metal or pressure-resistant plastic.

Inside the housing 60.70, a metal or plastic vibro 1, 7181 is provided as in the first embodiment, and a flexible plastic tube 62 is connected between these vibros 1 and 81. , pipe 81
．． A tube 72 is connected between the tube 71 and the tube 71 .

The tubes 62 and 72 are made of the same material as in the first embodiment, and are equipped with an O-ring 66 and a stopper 67 to secure each vibro 1.
, 81.71.

These vibros 1, 81, 71 and tubes 62, 72 form a flow path 5 having a backflow prevention function.

As the liquid sending unit main body 80 connected to these channels 5, a liquid sending unit of a bellows pump is used. Air vent valves 6373 are attached to the upper parts of the housings 60 and 70, respectively, and piping 64.74 attached to the lower part of the housing 60.70 is connected to the piping 65.75, respectively.
It is connected to a pump 4 of a pressure reduction mechanism.

This pump 4 is the same as that of the first embodiment.

Next, the operation of the second embodiment will be described.

Within the housings 60 and 70 are air vent valves 63.73.
After filling the housing with liquid such as water or oil, the air vent valve 63.73 is closed to make the inside of the housing 60.70 a closed system.

The pump 4 is operated to move the liquid in the housing 70 to the housing 60.

The liquid in the housing 60 increases, and the plastic tube 62 is compressed by this increased liquid and contracts to block the flow path 5.

At the same time, the liquid inside the housing 60 decreases, and the housing 7
The tube 72 inside the tube 72 expands and the flow path 5 is opened. At this time, if the bellows 80 is pulled up at the same time, the fluid to be sent is sucked into the bellows 80.

Next, when the flow of liquid by pump 4 is reversed, tube 72 within housing 70 contracts to prevent backflow of liquid. At the same time, the tube 62 within the housing 60 expands and the flow path 5 is opened.

At this time, when the bellows 80 is pushed down, the liquid stored in the bellows 80 is sent out into the housing 60.

As in the first embodiment, by repeating the process of expanding and contracting the tubes 62, 72 and bellows 80 of the housing 60.degree. 70, the cell suspension is continuously transferred.

The transfer rate of fluid such as cell suspension using this pump can be set to any desired value by setting the volume inside the bellows, the flow rate of liquid by the pump of the pressurization/decompression mechanism, and the reversal interval of the flow direction. It is.

In addition, in this pump, the fluid is intermittently sucked in and discharged, resulting in so-called pulsating flow, but if pulsating flow is undesirable, two pumps can be placed in parallel and operated in opposite phases. By doing so, pulsating flow can be prevented.

Further, in the present pump, a piston pump that is relatively gentle in mechanical impact can also be used for the liquid feeding unit main body.

According to this embodiment, the following effects occur.

That is, in this embodiment, the pump IA is constituted by the tubes 62 and 72 in the two housings 60.70 and the bellows 80. In addition to the effects of the first embodiment, the device is simpler and more compact.

It should be noted that the present invention is not limited to the above-described embodiments, but includes the following modifications and the like.

That is, in the first embodiment, the four housings were separated and connected by a flow path, etc., but the entire housing may be integrated and partitioned by partition plates to form each housing chamber. .

Further, in this embodiment, a gear pump is used to move the liquid within the housing, but a tube pump may also be used.

In addition, the specific structure, shape, etc. when implementing the present invention may be other structures, etc. within the range that can achieve the purpose of the present invention.

Effects of the Invention Since the pump and backflow prevention device according to the present invention are configured as described above, the following effects are produced.

In other words, it is an excellent technology that allows liquids containing floating substances that are easily destroyed by mechanical shock, such as cultured animal and plant cells, to be safely transferred or circulated for long periods of time without damaging these floating substances. effect occurs.

[Brief explanation of the drawing]

1 to 4 respectively show embodiments of the pump and backflow prevention device according to the present invention, FIG. 1 is an overall view of the pump of the first embodiment, FIG. 2 is a principle diagram of the same liquid feeding direction, FIG. 3 is a diagram of the operation of the pump of the pressurization/decompression mechanism, and FIG. 4 is a diagram of a second embodiment of the present invention. 1, IA...Pump, 2...Backflow prevention device, 3,5
...Flow path, 4...Pump (pressurization/reduction mechanism), 10゜2
0.30, 40, 60.70...Housing, 1.2
．． 32.42, 62.72...Plastic tube, 22...Bellows. Figure 2 (a)

Claims (2)

[Claims] (1) A plurality of housings are provided, including an inlet housing and an outlet housing equipped with a backflow prevention mechanism, and a liquid feeding unit housing, and are filled with liquid such as water and oil to form a sealed state, and the plurality of housings are A flow path passing through the housing is formed in between, and a check valve with a flexible tube connected to the flow path, and a liquid feeding section are formed, and a pressurization/depressurization mechanism is connected between each housing. A pump characterized in that it transports a cell suspension or the like by utilizing the expansion and contraction of the tube induced by alternately repeating pressurization and depressurization. (2) A check valve that has two housings that serve as an inlet and an outlet and are filled with liquid such as water and oil, and a flexible tube is connected to the inlet and outlet within the housing. is formed, and the flow path is opened and closed by utilizing expansion and contraction of the tube induced by alternately repeating pressurization and depressurization by a pressurization and depressurization mechanism connected to the housing. Prevention device.