JPH0446120B2 - - Google Patents
Info
- Publication number
- JPH0446120B2 JPH0446120B2 JP23265183A JP23265183A JPH0446120B2 JP H0446120 B2 JPH0446120 B2 JP H0446120B2 JP 23265183 A JP23265183 A JP 23265183A JP 23265183 A JP23265183 A JP 23265183A JP H0446120 B2 JPH0446120 B2 JP H0446120B2
- Authority
- JP
- Japan
- Prior art keywords
- concentration
- enzyme
- substrate
- reaction
- distribution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000004190 Enzymes Human genes 0.000 claims description 54
- 108090000790 Enzymes Proteins 0.000 claims description 54
- 239000000758 substrate Substances 0.000 claims description 34
- 238000006243 chemical reaction Methods 0.000 claims description 26
- 230000000694 effects Effects 0.000 claims description 25
- 239000000243 solution Substances 0.000 claims description 11
- 238000004458 analytical method Methods 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000010521 absorption reaction Methods 0.000 description 10
- 239000007795 chemical reaction product Substances 0.000 description 9
- 102000003992 Peroxidases Human genes 0.000 description 6
- 108040007629 peroxidase activity proteins Proteins 0.000 description 6
- 239000000427 antigen Substances 0.000 description 5
- 102000036639 antigens Human genes 0.000 description 5
- 108091007433 antigens Proteins 0.000 description 5
- 238000006911 enzymatic reaction Methods 0.000 description 5
- 238000002372 labelling Methods 0.000 description 5
- 230000000717 retained effect Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 4
- 238000004401 flow injection analysis Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical compound C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 description 3
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 2
- 238000006957 Michael reaction Methods 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23265183A JPS60126096A (ja) | 1983-12-12 | 1983-12-12 | フロ−インジエクシヨン分析方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP23265183A JPS60126096A (ja) | 1983-12-12 | 1983-12-12 | フロ−インジエクシヨン分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60126096A JPS60126096A (ja) | 1985-07-05 |
| JPH0446120B2 true JPH0446120B2 (https=) | 1992-07-28 |
Family
ID=16942634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP23265183A Granted JPS60126096A (ja) | 1983-12-12 | 1983-12-12 | フロ−インジエクシヨン分析方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60126096A (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7371741B2 (en) | 2003-05-28 | 2008-05-13 | Entremed, Inc. | Estradiol derivatives and pharmaceutical compositions using same |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101936909B (zh) * | 2010-08-31 | 2012-05-23 | 天津工业大学 | 一种微生物降解苯环的酶活测试方法 |
-
1983
- 1983-12-12 JP JP23265183A patent/JPS60126096A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7371741B2 (en) | 2003-05-28 | 2008-05-13 | Entremed, Inc. | Estradiol derivatives and pharmaceutical compositions using same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60126096A (ja) | 1985-07-05 |
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