JPH0436450Y2 - - Google Patents

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a syringe-shaped column used for chromatography, and more specifically, the invention relates to a syringe-shaped column used for chromatography. The homogeneity of the filler phase is not destroyed,
Therefore, the present invention relates to a syringe-type column with a novel structure in which the packing phase packed inside the column is not destroyed even when operations are performed to obtain high resolution.

(Prior Art) Recently, as chemical reactions become more precise, it has become necessary to develop a simple method for purifying and separating trace amounts of reaction products. One such method is
Medium-pressure chromatography using a syringe as a column has been considered. In this method, a syringe barrel with a discharge port at the tip is first filled with silica gel powder, alumina powder, cellulose fibers, etc., and then a solution containing the reaction products to be separated is placed in the barrel. A method in which a fixed amount is injected, and then a predetermined amount of a predetermined developing solvent is injected, a plunger is inserted into the barrel, the plunger is gradually pushed in, the target product is developed and separated, and it is collected from the outlet of the barrel. It is.

(Problems to be Solved by the Invention) When attempting to obtain high resolution using this method, the following inconvenient problems arise.

Generally, in order to purify and separate reaction products,
It is necessary to repeatedly inject the developing solvent here while maintaining the uniform filling state of the filler phase.
However, in the case of the method described above, the plunger must be withdrawn each time to repeatedly inject developing solvent into the barrel. When the plunger is pulled out of the barrel, there is always negative pressure inside the barrel. As a result, air flows in from the discharge port at the tip of the barrel, and the movement of this air disturbs the filling state of the filler phase, destroying its equilibrium state.

The purpose of the present invention is to provide a syringe-shaped column having a structure that does not cause the above-mentioned inconveniences when repeatedly injecting the developing solvent, and allows repeated injections of the developing solvent while maintaining the equilibrium of the packing phase. .

(Means for Solving the Problems) The syringe-shaped column of the present invention has a cylindrical barrel body having a solution discharge portion at one end and an open end at the other end; an inserted plunger body, through which at least one vent hole passes in the axial direction;

The column of the present invention will be explained in more detail with reference to the drawings. FIGS. 1 and 2 each represent a longitudinal sectional view of a cylindrical barrel body and a plunger body, which are combined to form a column.

The cylindrical barrel body has, for example, a cylindrical overall shape, and a solution discharge part 1a is provided at the bottom of the tip thereof, and an open end 1b is formed at the upper end. In addition, the plunger 2 has an upper surface 2b to a lower surface 2.
At least one ventilation hole 2a penetrates in the axial direction up to c.

The column of the present invention is operated as follows.
As shown in FIG. 3, first, the outlet of the solution discharge portion 1a of the cylindrical barrel body 1 is sealed with a liquid-permeable member 3 such as cotton. Then, a predetermined amount of filler 4 is filled here. The packing material may be appropriately selected depending on the purpose, from those used for ordinary column chromatography to those for HPLC. Thereafter, a predetermined sample solution is injected from the open end 1b, and further a predetermined amount of a predetermined developing solvent 5 is injected.

Then, insert the plunger 2 into the open end 1b,
Press fit the plunger 2 into the barrel body 1 while holding the upper surface 2b with your fingers.

As the plunger 2 is press-fitted, the developing solvent 5 permeates into the filler phase 4, and as a result, the target substance is eluted. When the plunger 2 comes into almost contact with the upper surface 2b of the plunger 2, release the finger pressing the upper surface 2b of the plunger 2 and pull out the plunger 2.

At this time, since air flows in from the vent hole 2a, the inside of the barrel body 1 does not become negative pressure. Moreover, since the air flows in through the vent hole 2a of the plunger 2 rather than through the solution discharge part 1a, the filler phase 4 is not disturbed by the movement of the incoming air.

FIG. 4 shows another embodiment of the plunger,
It has two ventilation holes passing through it. In the case of this plunger, as mentioned above, plunger 1
The developing solvent can be injected from the other vent hole 2'a without pulling out the barrel from the barrel.

Note that these cylindrical barrel bodies, plunger bodies, etc. may be made of glass, or may be made of resin such as polyethylene.

(Effects of the invention) As is clear from the above explanation, the column of the invention allows the developing solvent to be repeatedly injected without disturbing the equilibrium of the packing phase, and brings out high resolution of the target object. Its practical value is extremely great.

[Brief explanation of the drawing]

1 and 2 are longitudinal sectional views of a cylindrical barrel body and a plunger, respectively, which are one member of the column of the present invention. FIG. 3 is a diagram for explaining how to use the column of the present invention. FIG. 4 shows another embodiment of the plunger, which is one member of the column of the present invention. 1... Cylindrical barrel body, 1a... Solution discharge part, 1
b...Open end, 2...Plunger, 2a, 2'a
...Vent hole, 2b...Top surface, 2c...Bottom surface, 3...
... sealing member, 4 ... filler phase, 5 ... developing solvent.

Claims (1)

[Scope of utility model registration request] A cylindrical barrel body having a solution discharge part at one end and an open end; and a cylindrical barrel body inserted from the open end, with at least one ventilation hole passing through it in the axial direction. A syringe-shaped column for chromatography consisting of a plunger body.