JPH04357946A - Biotissue freezing device - Google Patents

Abstract

PURPOSE:To provide the biotissue freezing device which is provided with a treating needle constituted to allow direct puncture into the affected part of the bioorgans from the outside of the body without incising the abdomen and to allow the introduction of biotissue freezing fluid to its tip part and can maintain the vacuum degree of the vacuum space provided on the inside wall side of the outside pipe of this treating needle over a long period of time. CONSTITUTION:This biotissue freezing device has the treating needle 1 constituted to allow the introduction of biotissue freezing fluid into its tip part and has a tubular body 6 formed to the diameter larger than the diameter of this treating needle on the base side of the treating needle 1. A freezing fluid introducing pipe 3 and a discharge pipe 4 are penetrated in the tubular body penetrating in this tubular body and a vacuum is maintained in the tubular body 6 having the large diameter. In addition, active carbon 4 is sealed into the large- diameter tubular body.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a living tissue freezing device, and more particularly to a living tissue freezing device equipped with a cryotherapy needle that is inserted into the body to freeze and necrotize the affected part of the body's internal organs without having to open the abdomen.

0002

BACKGROUND OF THE INVENTION Conventionally, surgery for cancer of internal organs, such as liver cancer, liver tumor, and pancreatic cancer, has been performed through laparotomy and removal. Doctors who perform surgery are required to have the skill to deal with the risks of the surgery and the complications of complications such as visceral adhesions after surgery, and patients undergoing surgery are required to have the ability to withstand the long duration of the surgery. Open surgery is difficult in all respects, as it requires physical strength from the patient and also takes into account the concerns and considerations of the patient's family, relatives, and friends. On the other hand, it has become possible to clearly resolve images of affected parts of the body using ultrasound diagnostic equipment, making it easier to detect early cancers. This also limited the frequency of open surgery. In addition, although there is a treatment method in which the abdomen is opened and the affected area is directly viewed and frozen and necrotized using a cryotip using liquid nitrogen evaporation and adiabatic expansion, it requires a laparotomy first, and furthermore, only the tip of the cryotip is needed. However, the temperature is not low enough to cause frost to form on the connecting flexible pipe from the liquid nitrogen container to the frozen chip, and on the frozen chip holding tube.
Due to the ice that had adhered to the holding tube, it was impossible to insert the needle into the body.

For example, in the surgical pump disclosed in Japanese Utility Model Publication No. 52-40618, liquid nitrogen is injected into the tip of the outer tube through a refrigerating fluid jetting pipe disposed inside the outer tube, which can create a vacuum inside. The tube is guided into the space inside the tip cap fixed to the tube, and the tip cap is cooled by evaporation and adiabatic expansion, and the cooled tip cap is brought into contact with the affected area and frozen. This surgical procedure has been used with good results in surgeries for basal ganglia, intracerebral tumors, pituitary glands, intracavity sarcomas, etc.

0004

However, when the above-mentioned prior art is examined in detail, it is found that the gas cylinder has a vaporized refrigerant discharge pipe inserted into the gas cylinder end cap in the center of the outer tube, and A refrigerating fluid ejection pipe is disposed below the discharge pipe, and is supported by a plurality of support rings so that the positional relationship between these two pipes within the outer tube is stabilized. The outer peripheral wall of the support ring has a diameter that closely contacts the inner peripheral wall of the outer tube, and the support ring faces the outer tube. That is,
Even if the inside of the outer tube is maintained in a vacuum and convective heat transfer is blocked, in addition to the cold radiation reaching the outer tube from the vaporized refrigerant discharge pipe and the frozen fluid jet pipe, the cold heat of both pipes is transmitted through the plurality of support rings. There is a possibility that frost and ice may be formed on the outer circumferential wall of the outer tube at least at the portion where the support ring is in contact with the outer tube. Furthermore, the end face shape of the probe tip cap is a spherical surface with a radius equal to the diameter of the outer tube;
The only way to reach the affected area is to make an incision in the epithelium or biological tissue of the affected area of the patient, or to insert the probe tip cap to the affected area from a pre-opened area such as the oral cavity or oral cavity through the insertion tube.

In view of the drawbacks of the prior art, the present invention provides a therapeutic needle that can be inserted directly into the affected part of a living organ from outside the body without having to open the abdomen, and that can also introduce a fluid for freezing living tissue into its tip. The object of the present invention is to provide a living tissue freezing device that can maintain the degree of vacuum in the vacuum space provided on the inner wall of the outer tube of the therapeutic needle for a long period of time, and in which the therapeutic needle is easy to operate. do.

[0006]

[Means for Solving the Problems] In order to achieve the above-mentioned technical problems, the present invention provides a biological tissue freezing device equipped with a therapeutic needle capable of introducing a biological tissue freezing fluid into the distal end portion of the therapeutic needle. A tubular body having a diameter larger than that of the treatment needle is provided on the proximal side, and a freezing fluid introduction pipe and a discharge pipe are provided in the tubular body penetrating the tubular body, and a vacuum is provided in the tubular body of the large diameter. We propose a biological tissue freezing device characterized in that the biological tissue is maintained at a temperature of 100 mm, and activated carbon is enclosed within the large-diameter tube.

[0007] Furthermore, a flexible joint valve for evacuation having an appropriate length is provided inside the large-diameter tube, and a freezing fluid introduction pipe is provided through the base side of the large-diameter tube. It is preferable to install a cryopot connected to the introduction pipe via a pipe. Further, a discharge pipe is branched and inserted on the upstream side of the flexible pipe, the discharge pipe is connected to an exhaust pump, the pump and the cryopot are mounted on a transport wagon, and the cryopot and the flexible pipe are connected to each other. It is even more preferable if a universal joint is interposed between the two.

[0008]

[Operation] According to this technical means, the therapeutic needle has a discharge pipe and a freezing fluid introduction pipe passing through the large diameter tubular body, and has an outer tube that is connected and sealed to the large diameter tubular body, If the annular space formed between the outer pipe and the discharge pipe is configured to be connected to the vacuum space of the large diameter pipe, the vacuum space volume of the large diameter pipe is much larger than the volume of the annular space. Because of this, even if there is some vacuum leakage, the degree of vacuum in the annular space can be maintained for a long period of time. Moreover, by enclosing activated carbon in the vacuum space, the degree of vacuum in the annular space can be maintained for a longer period of time.

[0009] Furthermore, since a vacuum evacuation universal joint valve of an appropriate length is attached to the large-diameter pipe, the members used in the joint valve that cannot withstand relatively low temperatures can be replaced with large-diameter pipes that are at low temperatures. It becomes possible to install it in a place far away from the body,
The degree of vacuum in the vacuum space/annular space can be maintained for a long period of time without impairing the function of the member. In addition, by connecting the large diameter tube and the cryopot with a flexible tube through which the freezing fluid introduction tube is inserted, and by interposing a flexible joint between the cryopot and the flexible tube, it is possible to maintain a fixed position. The living tissue freezing device can be operated without being obstructed by the cryopot installed in the body. Furthermore, since the exhaust pump and cryopot are mounted on the transport wagon, they can be installed near the patient, and the living tissue freezing device can be easily operated.

0010

DESCRIPTION OF THE PREFERRED EMBODIMENTS Preferred embodiments of the present invention will be described in detail below by way of example with reference to the drawings. However, unless otherwise specified, the dimensions, materials, shapes, and relative positions of the components described in this example are not intended to limit the scope of this invention, but are merely illustrative. Just an example.

1 to 4 show a therapeutic needle for freezing a living body according to an embodiment of the present invention, FIG. 1 is a front cutaway sectional view showing the overall configuration including related members, and FIG. 2 shows a needle for freezing a living body according to an embodiment of the present invention. FIG. 3 is a front sectional view of the tip of the needle, FIG. 3 is a front view showing a portion of the duct in the middle thereof, and FIG. 4 is a side view thereof. Reference numeral 1 designates a treatment needle for freezing living organisms, which consists of a concentric triple-tube needle body 1a consisting of an inner tube 3, a middle tube 4, and an outer tube 5, and a tip member 2 sealed to the tip of the needle body 1a.

[0012] At the tip of the needle body 1a, the tip of the middle tube 4 is expanded in diameter toward the inner wall of the outer tube 5 in a figure-eight shape and sealed by welding, and the joint part is fitted into the rear part of the tip member 2. The distal end nozzle portion 3a of the inner tube 3 is sealed and extends into a conical cavity 2a provided inside the rear end of the distal end member 2. The tip of the tip member 2 is cut obliquely with respect to the center of the outer tube, and the oval outer edge on the tip side is further polished to provide a cutting edge 2b, and the tip member 2 has approximately the same diameter as the outer diameter of the needle body 1a. Together with the needle body 1a, it is formed so that it can be inserted into the body without opening the abdomen. In addition, a fiber thread made of resin such as polyethylene or polyester is spirally wound around the outer periphery of the inner tube 4, and a gap maintaining member 5 is formed between the inner tube 4 and the outer tube 5.
The annular space 15 between the inner tube 4 and the outer tube 5 communicates with a large-diameter tube vacuum space 16, which will be described later, via a retaining ring 12A to provide vacuum suction.

A tapered portion 6a is formed at the distal end of the large diameter tube 6, and a joint portion 6b is formed at the rear end, and the outer tube 5 of the needle body 1a is inserted into the tapered portion 6a and sealed by welding. It has been stopped. The annular annular space 15 formed between the outer tube 5 and the middle tube 4 is the large diameter tube vacuum space 1 in the large diameter tube body 6.
6, and the large-diameter tube vacuum space 16 communicates with a vacuum exhaust pipe 8 and a vacuum flexible joint 7 provided at the rear of the large-diameter tube body 6 for vacuum suction. In addition, the middle pipe 4 has a retaining ring 12A.
The return gas ring passage 17 formed in the gap between the inner wall of the middle pipe 4 and the outer wall of the inner pipe 3 is held by the exhaust pipe 10 at the rear of the large diameter pipe 6 and sealed by welding. It opens into the exhaust pipe 10 and further communicates with the exhaust pipe 9. In addition, the inner tube 3 that has passed through the exhaust pipe 10 is held by a retaining ring 12B provided at the joint portion 6b, and is guided to a flexible pipe 11 having an inner diameter larger than that of the inner tube 3, and reaches the cryopot 18. . On the outer wall of the inner tube 3 that passes through the exhaust pipe 10 and reaches the cryopot 18, a tape-shaped heat insulating cloth 1 made of laminated aluminum foil and nylon threaded woven cloth is provided.
3 is wound. Furthermore, activated carbon 14 is contained in the large diameter tube vacuum space 16.

[0014] As described above, the therapeutic needle 1 for freezing a living body and the large diameter tube body 6
, and the flexible pipe 11 are configured,
An annular space 15 formed between the outer tube 5 and the middle tube 4, a large diameter tube vacuum space 16 formed between the large diameter tube body 6 and the middle tube 4, and an annular space between the flexible pipe 11 and the inner tube 3. 11b are in communication with each other, and if a vacuum pump (not shown) is connected to the vacuum universal joint 7 and the three spaces are suctioned through the vacuum exhaust pipe 8 and the vacuum flexible joint 7, the three spaces will have a vacuum degree of 10- A vacuum of 6 to 10-7 torr can be created. The volumes of the remaining two spaces are extremely large compared to the annular space 15, so that even if there is some vacuum leakage, the degree of vacuum will not drop easily, and the activity inside the large diameter tubular body 6 will be reduced. By adsorbing carbon-14 molecules, this degree of vacuum can be maintained for a long period of time.

Next, each tube of the concentric triple tube forming the needle body 1a will be explained in order.The inner tube 3 is a stainless steel thin tube with an outer diameter and an inner diameter of approximately 1.0 mm and 0.8 mm, respectively. The tip nozzle portion 3a extends into a conical cavity 2a provided inside the rear end of the tip member 2, and the liquid nitrogen 31 flowing inside the inner tube 3 is ejected from the nozzle portion 3a to the tip member 2. It is configured to collide with the inner wall surface and vaporize. The inner diameter of the middle tube 4 is approximately 1.8 mm and 1.4 m, respectively.
The vaporized nitrogen is refluxed through a return gas ring 17 formed in the gap between the inner tube 3 and the middle tube 4 through a stainless steel thin tube of m. The outer tube 5 has an outer diameter and an inner diameter of approximately 3.0 mm and 2
．． The annular space 15 formed in the gap between the inner tube 3 and the outer tube 5 is communicated with a large diameter tube vacuum space 16 of the large diameter tube body 6, which is a 4 mm rigid stainless steel tube. Further, the length of the concentric triple tube is about 200 mm, and it is configured to be inserted into the body together with the tip member 2. The large diameter tube 6 is a stainless steel tube with an outer diameter of about 25 mm and a length of about 200 mm.

According to this therapeutic needle, when the exhaust pipe 9 is suctioned and depressurized by the exhaust pump 23, the liquid nitrogen 31 is introduced through the inner tube 3, and the liquid nitrogen 31 is discharged through the nozzle portion 3a.
is injected into the conical space 2a on the inner surface of the tip member 2, collides with the tip member 2 while expanding, and after the heat of evaporation acts as freezing heat, the heat-absorbing and vaporized gas reverses and hits the outer wall of the inner tube 3. It passes through the return gas ring path 17 in the inner gap of the middle pipe 4 and is discharged as return nitrogen gas 32 to the exhaust pipe 9 and the flexible exhaust pipe 2.
5 and is exhausted by the exhaust pump 23. At this time, since the liquid or gas flows through the return gas ring path 17 under a positive pressure state, there is no need to provide a holding member, and rather the presence of a holding member is not preferable because it creates resistance. Further, even if the inner tube 3 and the middle tube 4 come into contact with each other, the influence of heat loss is small because they are at low temperatures.

Since this point is already known, a detailed explanation of the structure will be omitted, and the resin thread gap holding member 5a, which is the main part of the present invention, will be explained. As shown in FIG. 3, resin threads having a diameter a approximately 1/2 A are wound around the middle tube 4 in opposite directions or at different winding pitches so as to cross each other. The point where the two threads overlap at the intersection 5b is configured to serve as a holding fulcrum. That is, since the diameter a of the pair of gap holding members 5a is approximately 1/2A, FIG.
As shown in FIG. 4, the two pipes are not thermally connected to each other over the entire length of the annular space 15 other than the intersection 5b;
Point b serves as a support point that holds the middle tube 4 and the outer tube 5 concentrically.

FIG. 5 is a perspective view showing the configuration of a cryo-needle treatment apparatus including the treatment needle for freezing a living body according to an embodiment of the present invention, in which 18 is a cryopot which is a thermos flask filled with liquid nitrogen 31; 24 is a switch for operating the exhaust pump 23; 22 is a switch for operating the cryopot 18, the exhaust pump 23, and the switch 24; 24 is a switch for operating the exhaust pump 23; This is a wagon car equipped with the vehicle.

Flexible pipe 11 containing inner pipe 3
The distal end portion is the joint portion 6b on the rear end side of the large diameter tube body 6.
The rear end side of the flexible pipe 11 is welded to the outer diameter of the tip of the liquid feed pipe 19, and the flexible pipe 1
The inside of the annular space 11b formed between the inner tube 1 and the inner tube 3 is airtightly sealed and fixed while communicating with the large diameter tube vacuum space 16. The inner pipe 3 is connected to a liquid feed pipe 19 whose outer peripheral wall surface is insulated with a heat insulating material, and the liquid feed pipe 19 is connected to a siphon (not shown) that reaches approximately the bottom of the cryopot 18 via a universal joint 21. The liquid nitrogen 31 connected and filled in the cryopot 18 is configured to be supplied to the treatment needle 1 for freezing a living body.

The exhaust pump 23 is connected to the exhaust pipe 9 of the treatment needle 1 for freezing living organisms via a flexible exhaust pipe 25.

6 and 7 show a treatment method performed using a treatment needle for freezing living bodies according to an embodiment of the present invention, FIG. 6 is a front view showing the concept of the treatment method, and FIG. 7 is a CR used for treatment.
It is a front view of a T screen. 1 is the treatment needle for freezing a living body;
The ultrasonic sensor 41 inserted into the human body 44 via the holding/guide fitting 42 and held by the holding/guiding fitting 42 and placed in contact with the human body 44 emits a transmission wave 47 to the affected part 45 of the human body 44. Then, reflected waves 48 from human organs including the affected part 45 are received. 50 is a CRT screen;
The screen is configured to display a baseline image 51 of ultrasonic transmission and reception, an image 52 of the treatment needle, and an image 55 of the affected area.

To explain the operation of the cryo-needling treatment apparatus configured as described above, while viewing the baseline image 51 and the image 55 of the affected area on the CRT screen 50, the treatment needle 1 for freezing the living body is inserted into the human body 44. Insert it toward the affected area 45. Since the image 52 of the therapeutic needle 1 is also displayed on the CRT screen 50, the holding/guide fitting 42 is operated until the baseline image 51 and the image 52 of the therapeutic needle match up to a matching point 53. Next, when the switch 24 is operated to drive the exhaust pump 23, the flexible exhaust pipe 25, exhaust pipes 9, 10
, and the gas in the return gas loop 17 is sucked in and exhausted,
The pressure within the conical cavity 2a of the tip member 2 becomes negative pressure. Therefore, the liquid nitrogen 31 in the cryopot 18 is
9, flows through the inner tube 3, is ejected in the form of a spray from the nozzle portion 3a, collides with the inner wall surface of the tip member 2 and vaporizes, and the sharp portion 2
is cooled to -130 degrees after approximately 1 minute, and an ice ball 46 is formed on the outer periphery of the sharpened portion 2, and the ice ball has a diameter of approximately 10 to 15 mm after approximately 1 minute, and approximately 10 to 15 mm in diameter after approximately 10 minutes. Grows to 20-30mm.

Ice ball image 5 on CRT screen 50
6 and when the desired size of the ice ball 46 is reached, operate the switch 24 to turn off the exhaust pump 23.
interrupts driving. The supply of liquid nitrogen 31 is stopped, the ice ball 46 formed on the tip member 2 is thawed by the body fluid, and the treatment needle 1 can be removed from the affected area 45. The treatment needle 1 is reinserted as necessary, and the above operation is repeated until the remaining living tissue in the affected area 45 is frozen and necrotized.

Furthermore, since the outer diameter of the tip member 2 and the needle body 1a is as small as about 3 mm, they can self-recover even if bleeding occurs.Moreover, since the needle body 1a is insulated by the annular space 15, the sharp point 2 When the treatment needle 1 for living body freezing is inserted and removed, organs on the way to the affected organ will not be damaged by freezing.

It should be noted that the liquid nitrogen 31 flowing into the inner tube 3 is blocked from radiant heat radiation by the heat insulating cloth 13 and from convective heat radiation by the annular space 11b maintained in a vacuum inside the flexible pipe 11. In the living body freezing treatment needle 1, a return gas ring path 1 is formed between the inner wall of the middle tube 4 and the inner wall of the inner tube 4.
7 is protected by low-temperature gaseous nitrogen refluxing, and the needle body 1
Inside a, it is insulated by the vacuum of the annular space 15 formed between the outer tube 5 and the inner tube 4, so the cold heat is dissipated to the outside air and no excess evaporation occurs. Nitrogen 31 can be supplied to the tip nozzle portion 3a without heat loss.

Furthermore, since the freezing needle device is entirely mounted on the wagon 22, the device can be placed close to the patient during treatment, and moreover, the freezing needle device 1 can be placed close to the patient during treatment. The devices installed on the wagon 22 include a flexible pipe 11 and a flexible exhaust pipe 25.
Since the needles 1 and 2 are connected with each other, the treatment needle 1 for freezing a living body can be easily operated.

Conventionally, medical treatment has been performed while monitoring images of cell collection probes and injection needles using an ultrasonic diagnostic device, but the treatment method of the present application uses the therapeutic needle for freezing living bodies in combination with an ultrasonic diagnostic device. Not yet established. However, since the growth process of the ice ball can be clearly seen on ultrasound echo images, it has become possible to treat it without having to open the abdomen.

FIG. 8 is a front view showing a biological tissue freezing device according to another embodiment of the present invention. Reference numeral 1 designates a treatment needle for freezing a biological body, which is welded and sealed to a large diameter tube body 6. As shown in FIG. The inside of the large-diameter tubular body 6 is connected to an ultra-vacuum pump (not shown) via a vacuum flexible joint and sucked therein, and is maintained in a vacuum. The exhaust nitrogen gas 32 vaporized by the treatment needle 1 is exhausted through the exhaust pipe 9 and the flexible exhaust pipe 25 by an exhaust pump (not shown). The therapeutic needle 1 is connected to the cryopot 18 via a joint nut 61 and an insulated straight pipe 62 provided at the rear end of the large diameter tube 6.

[0029] The biological tissue freezing device configured as described above has the following features:
When the lever 63 is pressed down to drive the exhaust pump and discharge the exhaust nitrogen gas 32, the liquid nitrogen 31 in the cryopot 18 is introduced into the tip of the treatment needle 1. In particular, the biological tissue freezing device can freeze and insert intracerebral tumors, pituitary glands, and other brain tissues, since the therapeutic needle 1 can be inserted through the skull.
It is applied in brain surgery to cause necrosis.

[0030]

As described above, according to the present invention, the annular space formed between the outer tube and the middle tube of the treatment needle is
It is configured to communicate with the vacuum space of the large diameter tube body, and because the large diameter tube vacuum space is filled with activated carbon, the volume of the large diameter tube vacuum space is formed to be much larger than the volume of the annular space. Therefore, even if there is a slight vacuum leak, it is possible to maintain the degree of vacuum in the annular space for a long period of time, and the outer peripheral wall of the treatment needle will not freeze over a long period of time. It is possible to provide a living body freezing device that has a therapeutic needle that can be inserted into the body.

[0031]Furthermore, since a vacuum evacuation universal joint valve of an appropriate length is attached to the large-diameter tube, it is possible to install a member that cannot withstand low temperatures at a location away from the large-diameter tube, which is at a low temperature. It is possible to provide a biological freezing device that can maintain the degree of vacuum in the large-diameter tube vacuum space/annular space for a long period of time without impairing the function of the member. In addition, by connecting the large diameter tube and the cryopot with a flexible tube through which the freezing fluid introduction tube is inserted, and by interposing a flexible joint between the cryopot and the flexible tube, it is possible to maintain a fixed position. The living tissue freezing device can be operated without being obstructed by the cryopot installed in the body. Furthermore, since the exhaust pump and cryopot are mounted on the transport wagon, they can be installed near the patient, and the living tissue freezing device can be easily operated. It has various effects such as

[Brief explanation of drawings]

FIG. 1 is a front cross-sectional view showing the internal structure of a treatment needle for freezing a living body according to an embodiment of the present invention.

FIG. 2 is a front sectional view showing the main structure of the treatment needle for freezing living bodies according to the embodiment of the present invention.

FIG. 3 is a front view showing a portion of the midway pipe line of the treatment needle for freezing a living body according to the embodiment of the present invention;

FIG. 4 is a side view of FIG. 3;

FIG. 5 is a perspective view showing the configuration of a freezing needle treatment device including the treatment needle for freezing a living body according to an embodiment of the present invention.

FIG. 6 is a front view showing the concept of a treatment method performed using a treatment needle for freezing living organisms according to an embodiment of the present invention.

FIG. 7 is a front view of a CRT screen used for treatment using a treatment needle for freezing living organisms according to an embodiment of the present invention.

FIG. 8 is a front view showing a biological tissue freezing device according to another embodiment of the present invention.

[Explanation of symbols]

1 Treatment needle 3 Freezing fluid introduction pipe 4 Discharge pipe 6 Large diameter pipe 7 Universal joint valve for vacuuming 9 Discharge pipe 11 Flexible pipe 14 Activated carbon 16 Large diameter pipe vacuum space 18 Cryopot 21 Universal joint 22 Transport wagon 23 Exhaust pump

Claims (4)

[Claims] Claim 1: A biological tissue freezing device equipped with a treatment needle capable of introducing a biological tissue freezing fluid into its tip portion,
A tubular body having a larger diameter than the therapeutic needle is provided on the proximal side of the therapeutic needle, and a freezing fluid introduction pipe and a discharge pipe are provided in the tubular body penetrating the tubular body. A living tissue freezing device characterized by maintaining a vacuum inside the tube and encapsulating activated carbon in the large diameter tube. 2. The biological tissue freezing device according to claim 1, further comprising a vacuum evacuation universal joint valve having an appropriate length inside the large-diameter tube. 3. The living tissue according to claim 1, further comprising a cryopot connected to the introduction tube via a flexible tube having a freezing fluid introduction tube inserted therethrough, on the base side of the large-diameter tube body. freezing equipment 4. A discharge pipe according to claim 4, wherein a discharge pipe is branched and inserted on the upstream side of the flexible pipe, the discharge pipe is connected to an exhaust pump, and the pump and the cryopot are mounted on a transport wagon. 5. The living tissue freezing device according to claim 4, wherein a flexible joint is provided between the cryopot and the flexible tube.