JPH04357436A - Method and apparatus for diluting food sample for inspection of bacterial count - Google Patents
Method and apparatus for diluting food sample for inspection of bacterial countInfo
- Publication number
- JPH04357436A JPH04357436A JP40319990A JP40319990A JPH04357436A JP H04357436 A JPH04357436 A JP H04357436A JP 40319990 A JP40319990 A JP 40319990A JP 40319990 A JP40319990 A JP 40319990A JP H04357436 A JPH04357436 A JP H04357436A
- Authority
- JP
- Japan
- Prior art keywords
- dilution
- dilution water
- weight
- food sample
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000013305 food Nutrition 0.000 title claims abstract description 37
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 10
- 238000007865 diluting Methods 0.000 title claims abstract description 7
- 238000000034 method Methods 0.000 title claims description 12
- 238000007689 inspection Methods 0.000 title abstract 2
- 239000012895 dilution Substances 0.000 claims abstract description 100
- 238000010790 dilution Methods 0.000 claims abstract description 100
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 69
- 238000012360 testing method Methods 0.000 claims description 13
- 238000013459 approach Methods 0.000 claims description 4
- 239000012898 sample dilution Substances 0.000 claims description 2
- 238000003113 dilution method Methods 0.000 claims 1
- 239000011521 glass Substances 0.000 abstract description 2
- 239000004698 Polyethylene Substances 0.000 abstract 1
- -1 polyethylene Polymers 0.000 abstract 1
- 229920000573 polyethylene Polymers 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 1
- 238000013100 final test Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000021055 solid food Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Abstract
Description
【０００１】0001
【産業上の利用分野】本発明は、菌数検査を行うための
食品サンプルを、所定の希釈倍率で希釈するための方法
及びその装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method and apparatus for diluting a food sample at a predetermined dilution ratio for bacterial count testing.
【０００２】0002
【従来の技術】公定法に従って食品の菌数検査を行う場
合、まず食品サンプルを希釈する必要がある。このため
食品サンプルを正確に秤量して滅菌処理したサンプル容
器に投入し、さらに所定量の滅菌処理した希釈水を投入
して希釈し、次の工程（ミキシングまたはストマッキン
グ）に送られる。この希釈水は、事前に希釈倍率に応じ
て正確に秤量し、容器に入れて滅菌処理したものを多数
準備しておき、これを使用する。また場合によっては希
釈水をあらかじめサンプル容器容器内に準備しておき、
その中に正確に秤量した食品サンプルを投入することも
あるが、これらの作業のすべてが手作業によって行われ
ているのが現状である。BACKGROUND OF THE INVENTION When testing the number of bacteria on food according to the official method, it is first necessary to dilute the food sample. For this purpose, a food sample is accurately weighed and placed in a sterilized sample container, and then a predetermined amount of sterilized dilution water is added to dilute it before being sent to the next process (mixing or stomaching). This dilution water is prepared in advance in large quantities by accurately weighing it in accordance with the dilution ratio, placing it in a container, and sterilizing it. In some cases, dilution water may be prepared in advance in the sample container.
Accurately weighed food samples are sometimes put into the container, but currently all of this work is done manually.
【０００３】0003
【発明が解決しようとする課題】上記の通り、検査１件
あたり食品サンプルと希釈水を正確に秤量するという２
度にわたる秤量作業が必要であり、特に固体状の食品の
場合などでは、採取した食品サンプル量を細かく増減し
て正確な秤量を行うのに手間がかかるため、作業員の負
担が大きい。[Problems to be Solved by the Invention] As mentioned above, it is necessary to accurately weigh the food sample and dilution water for each test.
It requires repeated weighing operations, and especially in the case of solid foods, it takes time and effort to finely increase or decrease the amount of food sample taken to achieve accurate weighing, which places a heavy burden on the worker.
【０００４】またこれらはすべて手作業であるため、希
釈水を投入する際に、こぼれたり容器内に残ったりする
と希釈倍率に誤差を生ずることにもなり、作業員の個人
差や間違いが生じやすく、時間がかかり過ぎるなどの問
題がある。[0004] Furthermore, since all of this is done manually, if dilution water is spilled or remains in the container when adding dilution water, errors may occur in the dilution ratio, and individual differences and mistakes among workers are likely to occur. , there are problems such as it takes too much time.
【０００５】さらに希釈水を個々に準備しておいてこれ
を滅菌する必要があるが、その数が場合によっては数十
から数百にも及ぶため、滅菌設備が大形になる傾向があ
る。本発明は、食品サンプルを目分量で採取して、容器
収容部にセットされたサンプル容器に投入するだけの簡
単な作業により所定の希釈水が添加され、さらに正確な
希釈倍率を算出することができる、菌数検査用食品サン
プルの希釈方法とその装置を提供することを目的とする
。[0005] Furthermore, it is necessary to prepare and sterilize dilution water individually, but the number of dilution waters may range from tens to hundreds in some cases, so sterilization equipment tends to be large. According to the present invention, predetermined dilution water is added by a simple operation of taking a food sample by eye and placing it in a sample container set in a container accommodating part, and furthermore, it is possible to calculate an accurate dilution ratio. The purpose of the present invention is to provide a method and device for diluting food samples for bacterial count testing.
【０００６】[0006]
【課題を解決するための手段】上記目的は、次の方法及
び装置により達成される。[Means for Solving the Problems] The above objects are achieved by the following method and apparatus.
【０００７】請求項１の発明は、容器収容部にセットさ
れたサンプル容器の重量と、サンプル容器に食品サンプ
ルが投入されたときの重量を計測して食品サンプルの正
味重量を算出し、この正味重量からあらかじめ設定した
希釈倍率に相当する希釈水量および／または所定量の希
釈水添加後の総重量を算出し、希釈水を大小２種類の容
量をもつ２台の希釈水添加ポンプを使用して添加し、添
加された希釈水量が所定量に近付いたときに大容量の希
釈水添加ポンプを停止して小容量の希釈水添加ポンプの
みで希釈水を添加し、添加された希釈水量が所定量に達
したときに小容量の希釈水添加ポンプも停止して希釈を
終了し、最終のサンプル容器の総重量を計測して正確な
希釈倍率を算出して出力する菌数検査用食品サンプルの
希釈方法である。[0007] The invention of claim 1 calculates the net weight of the food sample by measuring the weight of the sample container set in the container accommodating part and the weight when the food sample is put into the sample container, and calculates the net weight of the food sample. From the weight, calculate the amount of dilution water corresponding to the dilution ratio set in advance and/or the total weight after adding a predetermined amount of dilution water, and add dilution water using two dilution water addition pumps with two different capacities. When the amount of dilution water added approaches the predetermined amount, the large-capacity dilution water addition pump is stopped and dilution water is added only with the small-capacity dilution water addition pump, and the amount of dilution water added reaches the predetermined amount. Dilution of food samples for bacterial count testing. It's a method.
【０００８】請求項２の発明は、容器収容部にセットさ
れたサンプル容器の重量を計測するための重量計測手段
と、重量計測手段からの重量信号を演算し、あらかじめ
設定された希釈倍率に相当する希釈水量および／または
所定量の希釈水添加後の重量を算出し、希釈水添加ポン
プの運転を制御するとともに実際の希釈倍率を算出する
ための演算制御回路と、希釈水を添加するための大小２
種類の容量をもつ２台の希釈水添加ポンプと、算出した
実際の希釈倍率を出力するための出力回路とを、有する
菌数検査用食品サンプルの希釈装置である。[0008] The invention of claim 2 includes a weight measuring means for measuring the weight of the sample container set in the container accommodating section, and a weight signal from the weight measuring means, which calculates a weight signal corresponding to a preset dilution ratio. an arithmetic control circuit that calculates the amount of dilution water to be added and/or the weight after adding a predetermined amount of dilution water, controls the operation of the dilution water addition pump, and calculates the actual dilution ratio; Large and small 2
This is a food sample dilution device for bacterial count testing, which includes two dilution water addition pumps with different capacities and an output circuit for outputting the calculated actual dilution ratio.
【０００９】[0009]
【作用】容器収容部にセットされたサンプル容器の重量
は、重量計測手段により計測されてその重量信号は演算
制御回路に送られる。サンプル容器に目分量で採取した
食品サンプルが投入されると、そのときの重量信号から
演算制御回路で食品サンプルの正味重量が算出され、あ
らかじめ設定した希釈倍率から所要の希釈水量および／
または希釈水添加後の重量が算出される。次いで大小２
種類の容量をもつ２台の希釈水添加ポンプを作動させて
希釈水を添加する。重量信号または容量信号により希釈
水量が所定量に近付いたら大容量の希釈水添加ポンプだ
けを停止する。さらに希釈水量が所定量に達したら小容
量の希釈水添加ポンプも停止して希釈を終了する。最終
の重量信号により実際の希釈倍率を算出してその値を出
力する。[Operation] The weight of the sample container set in the container accommodating section is measured by the weight measuring means, and the weight signal is sent to the arithmetic control circuit. When a food sample taken by eye is placed in a sample container, the arithmetic control circuit calculates the net weight of the food sample from the weight signal at that time, and calculates the required amount of dilution water and/or water from a preset dilution ratio.
Alternatively, the weight after addition of dilution water is calculated. Next, large and small 2
Two dilution water addition pumps with different capacities are operated to add dilution water. When the amount of dilution water approaches a predetermined amount based on the weight signal or volume signal, only the large-capacity dilution water addition pump is stopped. Further, when the amount of dilution water reaches a predetermined amount, the small capacity dilution water addition pump is also stopped to complete the dilution. The actual dilution ratio is calculated based on the final weight signal and the value is output.
【００１０】これにより、目分量で採取した食品サンプ
ルであっても所定の希釈倍率で希釈され、その正確な希
釈倍率が求められる。[0010] As a result, even a food sample taken by eye can be diluted at a predetermined dilution ratio, and an accurate dilution ratio can be determined.
【００１１】[0011]
【実施例】図１は本発明の一実施例を示す図であるが、
本発明はこの実施例に限定されるものではない。[Embodiment] FIG. 1 is a diagram showing an embodiment of the present invention.
The invention is not limited to this example.
【００１２】容器収容部１に滅菌済のガラス瓶、ポリ袋
などのサンプル容器２がセットされ、その重量は重量計
測手段３（本実施例にあってはロードセル）により計測
されて重量信号として演算制御回路４に送られる。空の
サンプル容器２がセットされているときの重量計測手段
３からの重量信号はＷ０ である。A sample container 2 such as a sterilized glass bottle or a plastic bag is set in the container storage section 1, and its weight is measured by a weight measuring means 3 (load cell in this embodiment) and calculated and controlled as a weight signal. Sent to circuit 4. The weight signal from the weight measuring means 3 when an empty sample container 2 is set is W0.
【００１３】サンプル容器２に食品サンプル５が投入さ
れると重量計測手段３からの重量信号はＷ１ となる。
このとき食品サンプル５を正確に秤量して採取する必要
はなく、本実施例にあっては約２０ｇを目分量で採取す
るだけでよい。When the food sample 5 is placed in the sample container 2, the weight signal from the weight measuring means 3 becomes W1. At this time, it is not necessary to accurately weigh and collect the food sample 5, and in this embodiment, it is sufficient to collect approximately 20 g by eye.
【００１４】次に希釈作業が行われるが、これは始動ス
イッチ（押ボタン）の操作によるか、重量計測手段３か
らの重量信号が一定値（Ｗ０ とＷ１ の間に適宜定め
る）を越えたのを検出することにより、サンプル５が投
入されたことを判別して始動させることができる。[0014] Next, dilution work is carried out, but this is done either by operating the start switch (push button) or when the weight signal from the weight measuring means 3 exceeds a certain value (appropriately determined between W0 and W1). By detecting this, it is possible to determine that sample 5 has been introduced and to start the operation.
【００１５】演算制御回路４は重量計測手段３からの重
量信号Ｗ０ 、Ｗ１ から食品サンプル５の正味重量Ｗ
ｓ（すなわちＷ１ −Ｗ０ ）を算出し、あらかじめ設
定した希釈倍率Ｎ０ （本実施例にあっては１０）の値
から希釈水量Ｗｗ（すなわちＮ０ ・Ｗｓ −Ｗｓ ）
および／または希釈水添加後の総重量Ｗ２ （すなわち
Ｗ１ ＋Ｗｗ またはＷ０ ＋Ｎ０ ・Ｗｓ ）を算出
する。このとき希釈倍率Ｎ０ はスイッチ等を利用して
所定値を設定する。The arithmetic control circuit 4 determines the net weight W of the food sample 5 from the weight signals W0 and W1 from the weight measuring means 3.
s (i.e., W1 - W0 ), and the dilution water volume Ww (i.e., N0 ・Ws - Ws ) from the value of the preset dilution ratio N0 (10 in this example).
And/or calculate the total weight W2 after addition of dilution water (ie, W1 +Ww or W0 +N0 ·Ws). At this time, the dilution ratio N0 is set to a predetermined value using a switch or the like.
【００１６】さらに演算制御回路４は大小２種類の容量
をもつ２台の希釈水添加ポンプ６、７に運転信号を送り
、希釈水添加ポンプ６、７は希釈水タンク８中の滅菌さ
れた希釈水９を添加ノズル１０を経てサンプル容器２中
に添加して希釈を開始する。本実施例では大容量の希釈
水添加ポンプ６として５００ｍｌ／分の容量のポンプを
、小容量の希釈水添加ポンプ７として１５０ｍｌ／分の
容量のポンプをそれぞれ使用した。Furthermore, the arithmetic control circuit 4 sends operation signals to two dilution water addition pumps 6 and 7 having two different capacities, and the dilution water addition pumps 6 and 7 supply the sterilized dilution water in the dilution water tank 8. Water 9 is added into the sample container 2 via the addition nozzle 10 to begin the dilution. In this example, a pump with a capacity of 500 ml/min was used as the large capacity dilution water addition pump 6, and a pump with a capacity of 150 ml/min was used as the small capacity dilution water addition pump 7.
【００１７】重量計測手段３からの重量信号がＷ２ に
近付いたら（この値はあらかじめ定めておくが本実施例
ではＷ２ の９５％とした）演算制御回路４からの停止
信号により大容量の希釈水添加ポンプ６を停止する。次
いで重量計測手段３からの重量信号がＷ２ に達したと
きに、演算制御回路４からの停止信号によって小容量の
希釈水添加ポンプ７も停止する。このとき重量計測手段
３からの重量信号に代え、実際に添加された希釈水量を
測定した信号Ｖ（希釈水添加ポンプ６、７に定容量ポン
プが用いられた時はその運転時間でもよい）と希釈水の
容量Ｖｗ（すなわちＷｗ）から希釈水添加ポンプ６、７
の運転制御を行うこともできる。When the weight signal from the weight measuring means 3 approaches W2 (this value is predetermined, but in this embodiment it is set to 95% of W2), a large volume of dilution water is sent by a stop signal from the arithmetic control circuit 4. Stop the addition pump 6. Next, when the weight signal from the weight measuring means 3 reaches W2, the small capacity dilution water addition pump 7 is also stopped by a stop signal from the arithmetic and control circuit 4. At this time, instead of the weight signal from the weight measuring means 3, a signal V measuring the amount of dilution water actually added (if a constant volume pump is used as the dilution water addition pumps 6 and 7, the operating time may also be used). From the dilution water volume Vw (i.e. Ww), the dilution water addition pumps 6, 7
It is also possible to control the operation of
【００１８】希釈水９の添加が終了したら、重量計測手
段３からの重量信号Ｗ３ を測定し、実際の希釈倍率Ｎ
１ を例えば次式により算出する。When the addition of the dilution water 9 is completed, the weight signal W3 from the weight measuring means 3 is measured, and the actual dilution ratio N is determined.
1 is calculated using the following formula, for example.
【００１９】Ｎ１ ＝（Ｗ３ −Ｗ０ ）／Ｗｓ本実施
例では、実際の希釈倍率Ｎ１ の値を９．９５〜１０．
０５の範囲に収めることができた。N1 = (W3 - W0)/Ws In this embodiment, the value of the actual dilution ratio N1 is 9.95 to 10.
I was able to keep it within the range of 0.05.
【００２０】実際の希釈倍率Ｎ１ は出力回路１１によ
って表示され、必要により記録、プリントアウトなどを
行う。The actual dilution ratio N1 is displayed by the output circuit 11, and recorded or printed out if necessary.
【００２１】[0021]
【発明の効果】本発明の方法および装置によれば、公定
法にしたがって食品の菌数検査を行う場合、食品サンプ
ルを目分量で採取してサンプル容器に投入するだけの簡
単な作業によって、自動により短時間で所定の希釈倍率
での希釈が実施でき、その正確な希釈倍率が求められる
ため、作業員の操作ミスや個人差を防止することができ
る。また正確な希釈倍率が表示、記録されることにより
最終の検査結果から菌数を算出するのが容易であり、さ
らにプリントアウトしたラベルをサンプル容器に貼付す
るようにすればサンプルの取り違いが起ることがない。
加えて希釈水の滅菌装置が簡単になるなど、工業的価値
は大なるものがある。[Effects of the Invention] According to the method and apparatus of the present invention, when testing the number of bacteria on food according to the official method, it is possible to automatically test the number of bacteria by simply taking a food sample by eye and putting it into a sample container. This allows dilution to be carried out at a predetermined dilution ratio in a short time, and since the accurate dilution ratio is determined, operator errors and individual differences can be prevented. In addition, by displaying and recording accurate dilution ratios, it is easy to calculate the number of bacteria from the final test results.Furthermore, by attaching a printed label to the sample container, there is no chance of sample mix-up. Never. In addition, it has great industrial value, such as simplifying the equipment for sterilizing dilution water.
【図１】本発明の一実施例を示す図。FIG. 1 is a diagram showing an embodiment of the present invention.
２・・・サンプル容器 ３・・・重量計測手段 ４・・・演算制御回路 ５・・・食品サンプル ６、７・・・希釈水添加ポンプ ９・・・希釈水 １１・・・出力回路 2...sample container 3...Weight measurement means 4... Arithmetic control circuit 5...Food sample 6, 7... Dilution water addition pump 9... Dilution water 11... Output circuit
Claims (2)
にあたり、食品サンプルを秤量した後、所定量の希釈水
を添加して食品サンプルの希釈を行う菌数検査用食品サ
ンプルの希釈方法において、容器収容部にセットされた
滅菌済のサンプル容器の重量と、サンプル容器に食品サ
ンプルが投入されたときの重量を計測して食品サンプル
の正味重量を算出し、この正味重量からあらかじめ設定
した希釈倍率に相当する希釈水量および／または所定量
の希釈水添加後の総重量を算出し、滅菌された希釈水を
大小２種類の容量をもつ２台の希釈水添加ポンプを使用
して添加し、添加された希釈水量が所定量に近付いたと
きに大容量の希釈水添加ポンプを停止して小容量の希釈
水添加ポンプのみで希釈水を添加し、添加された希釈水
量が所定量に達したときに小容量の希釈水添加ポンプも
停止して希釈を終了し、最終のサンプル容器の総重量を
計測して正確な希釈倍率を算出して出力することを特徴
とする菌数検査用食品サンプルの希釈方法。[Claim 1] In a method of diluting a food sample for bacterial count testing, the food sample is weighed and then a predetermined amount of dilution water is added to dilute the food sample when testing food for bacterial count according to the official method. The net weight of the food sample is calculated by measuring the weight of the sterilized sample container set in the container storage section and the weight of the food sample placed in the sample container, and the preset dilution ratio is calculated from this net weight. Calculate the amount of dilution water equivalent to and/or the total weight after adding a predetermined amount of dilution water, add sterilized dilution water using two dilution water addition pumps with two different capacities, and add When the amount of dilution water added approaches the predetermined amount, stop the large-capacity dilution water addition pump and add dilution water only with the small-capacity dilution water addition pump, and when the amount of dilution water added reaches the predetermined amount. The small-capacity dilution water addition pump is also stopped to complete the dilution, the total weight of the final sample container is measured, and an accurate dilution ratio is calculated and outputted. Dilution method.
ため、食品サンプルを秤量した後、所定量の希釈水を添
加して食品サンプルの希釈を行う菌数検査用食品サンプ
ルの希釈装置において、容器収容部にセットされたサン
プル容器の重量を計測するための重量計測手段と、重量
計測手段からの重量信号を演算し、あらかじめ設定され
た希釈倍率に相当する希釈水量および／または所定量の
希釈水添加後の総重量を算出し、希釈水添加ポンプの運
転を制御するとともに希釈終了後に実際の希釈倍率を算
出するための演算制御回路と、希釈水を添加するための
大小２種類の容量をもつ２台の希釈水添加ポンプと、算
出した実際の希釈倍率を出力するための出力回路とを、
有することを特徴とする菌数検査用食品サンプルの希釈
装置。[Claim 2] In a food sample diluter for bacterial count testing, which weighs the food sample and then adds a predetermined amount of dilution water to dilute the food sample in order to test the food sample according to the official method, A weight measuring means for measuring the weight of the sample container set in the container accommodating part, and a weight signal from the weight measuring means are calculated, and the amount of dilution water corresponding to a preset dilution ratio and/or the predetermined amount of dilution is calculated. An arithmetic control circuit that calculates the total weight after adding water, controls the operation of the dilution water addition pump, and calculates the actual dilution ratio after the completion of dilution, and two types of capacity, large and small, for adding dilution water. Two dilution water addition pumps and an output circuit to output the calculated actual dilution ratio,
A food sample dilution device for bacterial count testing, characterized in that:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP40319990A JPH04357436A (en) | 1990-12-18 | 1990-12-18 | Method and apparatus for diluting food sample for inspection of bacterial count |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP40319990A JPH04357436A (en) | 1990-12-18 | 1990-12-18 | Method and apparatus for diluting food sample for inspection of bacterial count |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04357436A true JPH04357436A (en) | 1992-12-10 |
Family
ID=18512953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP40319990A Pending JPH04357436A (en) | 1990-12-18 | 1990-12-18 | Method and apparatus for diluting food sample for inspection of bacterial count |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04357436A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011522235A (en) * | 2008-05-30 | 2011-07-28 | アエエス シェムニュー | Equipment for diluting samples |
| JP2020510194A (en) * | 2017-02-23 | 2020-04-02 | 鄭州安図生物工程股▲ふん▼有限公司 | Sample addition system that automatically adds sputum lysate |
| JP7245572B1 (en) * | 2022-12-21 | 2023-03-24 | 合同会社陶徳堂研究所 | body fluid collector |
-
1990
- 1990-12-18 JP JP40319990A patent/JPH04357436A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011522235A (en) * | 2008-05-30 | 2011-07-28 | アエエス シェムニュー | Equipment for diluting samples |
| JP2020510194A (en) * | 2017-02-23 | 2020-04-02 | 鄭州安図生物工程股▲ふん▼有限公司 | Sample addition system that automatically adds sputum lysate |
| JP7245572B1 (en) * | 2022-12-21 | 2023-03-24 | 合同会社陶徳堂研究所 | body fluid collector |
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