JPH0429414Y2 - - Google Patents

Description

[Detailed description of the invention] (a) Industrial application field This invention relates to an automatic analyzer, and relates to an automatic analyzer that analyzes the components of a specimen sample using a reaction with a reagent solution.

(B) Prior art In general, as shown in Fig. 1, a flow injector is used in which a liquid feeding pump 2, a reaction coil 3, and a detector 4 are arranged in this order in a liquid feeding path 1 for feeding a buffer solution and a reagent solution. In conventional automatic analyzers, plunger pumps, peristaltic pumps, etc. have been used as liquid pumps.

However, among these pumps, the plunger pump has a complicated structure, making the device itself complicated and expensive. On the other hand, although the peristaltic pump has a simple structure, the amount of liquid fed is not stable, which reduces the reproducibility and stability of the analytical data of the device.

(c) Purpose of the invention This invention was created in view of the above circumstances.
The aim is to improve the reproducibility and stability of analytical data, and to simplify the configuration of the apparatus.

(d) Structure of the device The structure of the device is such that a reagent solution supply channel equipped with a reagent solution supply means and a buffer solution supply channel equipped with a buffer solution supply means are connected to a sample liquid supply channel through a channel switching valve. A sample pipetter, a reaction coil, and a detector are arranged in this order in the sample feed path, and an operation control section is provided to control the operation of these components, and the sample liquid is connected to the sample liquid and the components of the sample are mixed with the reagent solution. The automatic analyzer performs analysis using the reaction of After pumping the liquid toward the reaction coil, operate the sample sample dispenser to inject one sample into the buffer and/or reagent solution, and transfer the reagent solution and buffer containing this sample sample to the reagent solution. This is an automatic analyzer consisting of a central control unit that operates the supply means or the buffer supply means to force-feed the buffer solution to the detector.

(e) Examples This invention will be described in detail below based on examples shown in the figures. Note that this invention is not limited to this.

FIG. 2 shows the configuration of an automatic analyzer 5, in which 6 is a buffer solution, 7 is a reagent solution, and a specimen sample dispenser 9, a reaction coil 10, and a detector 11 are arranged in this order in a sample delivery path 8 that delivers both of these solutions.
Reference numeral 2 denotes an autosampler that measures and sends a specimen sample to the specimen sample dispenser. The liquid feed path is branched into a reagent liquid feed path 14 and a buffer liquid feed path 15 via a flow path switching valve 13 just before the specimen sample dispenser 9. Flow path switching valves 16 and 17 are provided on both liquid feed paths, respectively, and a reagent liquid syringe 18 and a buffer liquid syringe 19 are connected to these switching valves, respectively. These syringes have a volume of liquid fed of 1000 ml.
0.5 to several ml/stroke. 20 is a central control unit (CPU) that automatically controls the device 5.
Three syringes 18, 19, three flow path switching valves 1
3, 16, and 17 are connected to the specimen sample dispenser 9 and the autosampler 12, and are configured to control the operation timing of these in conjunction with each other.

Next, a method for analyzing a specimen sample using the automatic analyzer 5 having the above configuration will be described.

First, the CPU 20 is activated, and the buffer solution syringe 19 and its flow path switching valve 17 are activated in correspondence with each other, and the buffer solution 6 is sucked into the syringe 19. Next, the reagent liquid syringe 18 and its flow path switching valve 16 are operated in a corresponding manner to aspirate the reagent liquid 7. Therefore, the flow path switching valve 1 at the branch part
3, the reagent liquid feeding path 14 is connected to the sample liquid feeding path 8.
Connect. Then, the reagent liquid syringe 18 is operated to feed the reagent liquid. At this time, a reagent zone 21 as shown in FIG. 3A is formed in the liquid feeding path 8, and the end of the reagent zone 21 in the advancing direction is fixed to the sample sample by the preset capacity of the reagent liquid syringe 18. It stops at a position beyond the dispenser 9. After the discharging operation of the reagent liquid syringe 18 is stopped, the specimen sample dispenser 9 is operated to dispense the specimen sample 22 measured by the autosampler 12 into the reagent zone 21. At this time, the specimen sample is in a state of being sandwiched in the reagent solution 7 as shown in FIG. 3B. Subsequently, after stopping the dispensing operation of the sample dispenser 9, the flow path switching valve 13 at the branch portion is switched from the reagent liquid feeding path 14 to the buffer liquid feeding path 15, and the buffer solution is fed to the sample liquid feeding path 8. Liquid path 15
Connect. Then, the buffer syringe 19 is operated again to discharge a preset amount of buffer. Then, the reagent zone 21 is pumped into one reaction coil, and the sample sample 22, reagent solution 7, and buffer solution 6 are
are mixed and reacted, and the reaction product is further pressure-fed toward the detector 11 and detected. Then, the entire amount of the buffer solution 6 in the buffer solution syringe 19 is discharged, and the reaction product is discharged to the outside of the sample liquid feeding path 8. Therefore, in order to analyze the next specimen sample, the buffer syringe 19 and its flow path switching valve 17 are operated again to aspirate the buffer 6. In this way, a large number of specimen samples are sequentially analyzed.

By configuring the automatic analyzer 5 as described above and analyzing one specimen with only one reciprocating movement of the reagent liquid syringe, the reproducibility of analytical data can be improved, and the apparatus itself can be constructed easily and inexpensively. Can be configured. Furthermore, by injecting the specimen sample into the reagent zone, the peaks of the analytical data become sharp, thereby improving the accuracy of the analysis. Furthermore, when a plurality of different reagent solutions are used, it is also possible to provide a plurality of reagent supply channels corresponding to these reagent solutions, and to install a reagent solution syringe in each of these reagent supply channels. Furthermore, the dispensing operation of the specimen sample dispenser is
This may be done either when the reagent solution is being delivered or when the buffer solution is being delivered.

Although it is different from this idea, one of the reagent syringes is
It is also possible to transfer multiple specimen samples in one stroke and perform multiple analyses.

(f) Effects of the device This device allows the supply flow paths for both liquids to be connected by switching separately, so the reagent liquid can be sent only when needed, reducing the wasteful consumption of expensive reagent liquids. can be prevented. Furthermore, since the feeding of the reagent solution can be made to correspond to the dispensing of the specimen sample, a sufficient reaction can be carried out with a small amount of the reagent solution, and the reproducibility and stability of analytical data can be ensured.

[Brief explanation of the drawing]

Figure 1 is a schematic configuration diagram showing a conventional automatic analyzer, Figure 2 is a diagram corresponding to Figure 1 showing an automatic analyzer according to this invention, Figure 3 A and B are buffer solutions in the sample liquid passage, In the state explanatory diagram showing the states of the reagent solution and the specimen sample, A indicates a reagent zone, and B shows a state in which the specimen sample is injected into this reagent zone. 5... Automatic analyzer, 6... Buffer solution, 7... Reagent solution, 8... Sample liquid feeding path, 9... Specimen sample dispenser, 10... Reaction coil, 11... Detector, 14
... Reagent solution feeding path, 15... Buffer solution feeding path, 1
6, 17...Flow path switching valve, 18...Reagent liquid syringe, 19...Buffer solution syringe, 20...Central control unit.

Claims (1)

[Scope of Claim for Utility Model Registration] 1. A reagent solution supply channel provided with a reagent solution supply means and a buffer solution supply channel provided with a buffer solution supply means are connected to a sample liquid supply channel via a channel switching valve. The sample liquid supply path is equipped with a sample dispenser, a reaction coil, and a sample container in this order, and is further equipped with an operation control unit that controls the operation of these components to cause the components of the sample sample to react with the reagent solution. In the automatic analyzer, the operation control section operates the reagent solution supply means or the buffer solution supply means in correspondence with the operation of the flow path switching valve, so that the buffer solution and the reagent solution are reacted. After pressure feeding toward the coil, the specimen sample dispenser is operated to inject one specimen into the buffer solution and/or reagent solution, and the reagent solution and buffer solution containing this specimen sample are transferred to the reagent solution supply means or An automatic analyzer characterized by comprising a central control section that operates a buffer supply means and controls the buffer solution to be fed under pressure to a detector. 2. The central control unit alternately operates the buffer solution supply means and the reagent solution supply means to force-feed the buffer solution and the reagent solution to the sample feeding path in order, and to fill each reagent solution separated by the buffer solution. 2. The automatic analyzer according to claim 1, wherein a specimen sample dispenser is controlled to inject one specimen sample so that a plurality of specimen samples can be sequentially analyzed. 3. The reagent solution supply means and the buffer solution supply means are each configured by connecting a corresponding supply channel and a channel communicating with the reagent solution or buffer solution to a syringe via a switching valve, and the respective syringe 2. The automatic analyzer according to claim 1, wherein the operation of the switching valve and the switching valve are controlled by the central control section.