JPH04285565A - Regenerative and inductive material for periodontium - Google Patents

Abstract

PURPOSE:To ensure safety, eliminate a need for reoperation after curing periodontitis, and lessen a pain and economical burden on a patient by using a material having high compatibility with a living body and very low foreign body reaction with the living body, and absorbable and decomposable in the living body. CONSTITUTION:A regeneration-inducible material to be suitably used for regenerative periodontium surgical therapy (GTR method) is obtained, comprising a mold (non-woven fabric, sponge or film type) of chitin (including deacetylated chitin and chitin derivative).

Description

[Detailed description of the invention]

0001

[Industrial Application Field] The present invention relates to regenerative periodontal surgery (G
uided tissue regeneratin
This article relates to periodontal tissue regeneration-inducing materials that are suitably used in periodontal tissue regeneration (GTR method), and specifically relates to biodegradable materials that are useful for regenerating living tissue eroded by periodontal disease and for treating periodontal disease. It relates to dental materials.

0002

[Prior Art] Regenerative periodontal surgery recommended by Linde and Nyman et al. is one of the treatment methods for periodontal disease [N
yman et al., J. Clin. Period. ,9
, 257-296 (1982)] is based on the concept that many damaged biological tissues can self-regenerate if given appropriate assistance. Conventionally, non-biodegradable Gore-Tex (trade name) has been used in periodontal tissue regeneration therapy. However, Gore-Tex (trade name), which is made of polytetrafluoroethylene, is non-biodegradable and is a foreign substance to the living body, so it is also tissue-reactive, so re-operation is required to remove it after treatment.

[0003] In consideration of the above points, a method using a biodegradable synthetic polymer material has recently been proposed (see JP-A-2-63465). However, these synthetic polymer compounds have poor water absorption properties and give a stiff and uncomfortable feeling when used. Furthermore, although the biodegradable synthetic polymer compounds mentioned above are degradable, they are recognized by living organisms as foreign substances, and some tissues have a strong foreign body reaction.

0004

[Problems to be Solved by the Invention] The purpose of the present invention is to improve the discomfort and compatibility with living organisms, which are the drawbacks of the synthetic polymer materials mentioned above, and to improve the compatibility with living organisms even if left in the living body after periodontal disease treatment. The object of the present invention is to provide a material for regenerative periodontal surgery that causes extremely little discomfort and foreign body reaction.

[0005]

[Means for solving the problem] The present inventors have discovered that crustaceans,
During research into the treatment of periodontal disease using chitin, which is widely found in nature, such as insects and mold, we discovered that chitin is excellent for regenerating periodontal tissues, and that it causes very little discomfort to living organisms and foreign body reactions to periodontal tissues. The invention was completed.

That is, the gist of the present invention is a periodontal tissue regeneration-inducing material, which is a molded article made of chitin.

The present invention will be explained in detail below. The chitin in the present invention includes chitin of any degree of deacetylation,
Alternatively, derivatives thereof may be used. Examples of chitin derivatives include etherified products, esterified products, carboxymethylated products, hydroxyesterified products, O-ethylated products, and the like. Specific examples include poly(N-acetyl-6-O-(2'-hydroxyethyl)-D-glucosamine, poly(N-acetyl-6-O-ethyl)-D-
Examples include glucosamine.

[0008] The molded article made of chitin of the present invention includes:
There are various forms such as nonwoven fabric, film, sponge, and knitted fabric. These can be molded by well-known methods. For example, after dissolving chitin in a well-known solvent such as dimethylacetamide, trifluoroacetic acid, sulfuric acid, or acetic acid, the desired molded article can be obtained.

An example of producing a sheet-like molded article will be shown below. Chitin fibers can be obtained by dissolving chitin in a well-known solvent to make a dope, extruding the dope through a nozzle with fine pores, coagulating it in a coagulating liquid such as isobutanol, and then pulling it with a roller through wet spinning. can. A nonwoven fabric is obtained by cutting the chitin fibers into pieces with a length of 1 to 5 mm, dispersing them in water, making paper using a paper machine, dehydrating them using a press, and drying them by heating.

To treat periodontal disease using the periodontal tissue regeneration-inducing material of the present invention, regenerative periodontal surgery by Linde and Nyman et al. [Nyman et al., J. et al. Clin. Per
io. , 9, 257-296 (1982)]. That is, the gingiva is incised and turned over, the tooth root surface is scraped and smoothed, and then the granulation tissue in the damaged (affected area) is removed. Next, after covering the removed portion with the material of the present invention, the peeled-up gingiva is returned to its original position and the incision is sutured. For more detailed specific methods, please refer to the training book (GORE-TEXR PERIODON) published by GORE-TEX.
AL MATERIAL WORKSHOP TRAI
NING MANUAL, 1988).

[0011]

[Examples] The present invention will be explained in more detail below with reference to Examples. Example 1 Chitin powder (manufactured by Shin Nippon Kagaku) was ground into 100 meshes, treated with 1N HCl for 1 hour, further heated in a 3% NaOH aqueous solution at 90°C for 3 hours, and then ground again in chitin powder. The calcium and protein contained in the material were removed, washed repeatedly with water, and dried. The obtained chitin was dissolved in a dimethylacetamide solution containing 8% by weight of lithium chloride to give a viscosity of 265 centipoise at 30°C to a concentration of 0.2% by weight.

This chitin was dissolved to a concentration of 8% by weight in a dimethylacetamide solution containing 8% by weight of lithium chloride. The obtained dope was filtered through a 1480-mesh wire mesh, left to defoam, and then placed in a tank, transported under pressure by a gear pump, and discharged through a nozzle with a diameter of 0.06 mm and 200 holes at a rate of 2.2 g/min. It was extruded into a butanol solution at 60°C to solidify it, and then taken up by a roller at a speed of 10 m/min. The obtained yarn was thoroughly washed with water and then dried to obtain a filament having a single yarn denier of 0.68 denier.

The obtained filament was cut into 5 mm pieces to obtain short fibers. This short fiber was treated in a 20% NaOH aqueous solution at 121° C. for 1 hour to perform a deacetylation reaction. After the reaction, the short fibrous chitin obtained by washing with water and drying had a degree of deacetylation of 40%.

The short fibrous chitin thus obtained was made into a nonwoven fabric by the following method. The device used was a sheet machine TAPPIT20503-71 manufactured by Toyo Seiki Seisakusho. First, 0.5 g of chitin fiber and 0.05 g of poval binder as a binder were dissolved and dispersed in 1 liter of water. Next, water was added to the dispersion liquid to make a total volume of 6 liters, and after uniformly dispersing the fibers, it was placed in a sheet machine, and after the water was filtered and removed, the top and bottom of the residue at the bottom of the sheet machine was sandwiched between qualitative filter paper. Afterwards, the water was removed by sandwiching it between rolls. Furthermore, using a press machine, 3 kg/cm
After pressing for 30 seconds at a pressure of 2.0° C., drying was performed at 120 to 130° C. for 6 minutes to obtain a nonwoven fabric. The thickness was 0.1 mm. This nonwoven fabric was sterilized with ethylene oxide gas.

The chitin nonwoven fabric obtained as described above was implanted in a beagle dog that had suffered from periodontal disease and had suffered from gingival recession. In other words, by covering the tooth root surface in a tent-like manner with a chitin non-woven fabric, the flap is returned and sutured to prevent the connective tissue from coming into contact with the tooth root surface and participating in the healing process. Observed.

As a result, most of the chitin nonwoven fabric was decomposed, although some of it remained. On the other hand, new attachment including supporting alveolar bone was formed around the implantation site, and healing of the periodontal disease was observed.

Example 2 A chitin nonwoven fabric with a degree of deacetylation of 65% was obtained in the same manner as in Example 1, except that the short fibers were treated in a 35% NaOH aqueous solution to carry out the deacetylation reaction. This nonwoven fabric was implanted in a beagle dog suffering from periodontal disease in the same manner as in Example 1, and the state of healing was observed after 4 months.

As a result, most of the chitin nonwoven fabric was decomposed and absorbed, periodontal ligament fibers and new bone were formed, and the periodontal disease was cured.

Example 3 The dope obtained in Example 1 was cast onto glass and dried to obtain a film-like material with a thickness of 0.1 mm. The degree of deacetylation of the chitin film was 3%. This film was implanted in a beagle dog suffering from periodontal disease in the same manner as in Example 1, and the state of healing was observed after 5 months.

[0020] As a result, although some of the chitin film remained, most of it was decomposed and absorbed, and periodontal disease was also observed to be cured.

[0021]

[Effects of the invention] When the periodontal tissue regeneration-inducing material of the present invention is implanted in the oral cavity, there is no foreign body sensation, it has excellent biocompatibility, has extremely low foreign body reaction to living bodies, and is decomposed and absorbed in living bodies. , it is extremely safe. Also,
After the periodontal disease has healed, there is no need for reoperation, reducing patient pain and financial burden.

Claims (1)

[Claims] 1. A periodontal tissue regeneration-inducing material, which is a molded article made of chitin.