JPH04188061A - Method and apparatus for observing hematobium parasite adult - Google Patents
Method and apparatus for observing hematobium parasite adultInfo
- Publication number
- JPH04188061A JPH04188061A JP31913890A JP31913890A JPH04188061A JP H04188061 A JPH04188061 A JP H04188061A JP 31913890 A JP31913890 A JP 31913890A JP 31913890 A JP31913890 A JP 31913890A JP H04188061 A JPH04188061 A JP H04188061A
- Authority
- JP
- Japan
- Prior art keywords
- light
- interference filter
- parasite
- observing
- specimen
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000045947 parasite Species 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims description 10
- 229910052736 halogen Inorganic materials 0.000 claims abstract description 12
- 150000002367 halogens Chemical class 0.000 claims abstract description 12
- 238000001514 detection method Methods 0.000 claims description 9
- 239000007850 fluorescent dye Substances 0.000 claims description 7
- 238000010186 staining Methods 0.000 claims description 6
- 241000242678 Schistosoma Species 0.000 claims description 3
- 239000000975 dye Substances 0.000 claims description 3
- 201000004792 malaria Diseases 0.000 claims description 3
- 238000007689 inspection Methods 0.000 abstract description 5
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 abstract description 3
- 238000004040 coloring Methods 0.000 abstract 5
- SMWDFEZZVXVKRB-UHFFFAOYSA-N Quinoline Chemical compound N1=CC=CC2=CC=CC=C21 SMWDFEZZVXVKRB-UHFFFAOYSA-N 0.000 abstract 2
- DZBUGLKDJFMEHC-UHFFFAOYSA-N acridine Chemical compound C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 abstract 2
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 1
- 238000012137 double-staining Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は住血性寄生成虫・マラリア等の検量を精度よく
、かつ安価な装置で行うＩ！察方法及びその装置に関す
るものである。[Detailed Description of the Invention] [Field of Industrial Application] The present invention is an I! This article relates to a method and device for the detection method.
住血性寄生成虫やマラリア原虫の検査は資料台に検体を
載せ、光源として高圧水銀灯を用い、この光源よりの光
を集光レンズ、ＦＩＴＣフィルター （３６０〜５００
ｎ■）及びコンデンサレンズを軽で前記検体を照射し、
この検体の上方には対物レンズ、及び５１５ｎｗ以上の
干渉フィルターを軽で観察されている。To test for schistocytic parasites and malaria parasites, place the specimen on a document stand, use a high-pressure mercury lamp as the light source, and pass the light from this light source through a condensing lens and an FITC filter (360 to 500
n■) and irradiating the specimen with a light condenser lens,
Above this specimen, an objective lens and an interference filter of 515 nw or more were observed with light.
従って従来の方法は高価な高圧水銀灯、ＦＩＴＣフィル
ター、検出用として５１５ｎｍ以上の干渉フィルターを
使用し、光源と資料台間にコンデンサーレンズを使用し
た高級顕微鯖を使用しなければならず、また検体染色用
として特定の蛍光色素としてギムザー染色を行っている
。Therefore, the conventional method requires the use of an expensive high-pressure mercury lamp, an FITC filter, an interference filter of 515 nm or more for detection, a high-quality microscope with a condenser lens between the light source and the data table, and the use of a high-quality microscope for staining the specimen. Giemser staining is performed using a specific fluorescent dye.
このため使用する顕微鏡が高価となり、しかもギムザー
染色を用いているので血液と住血性寄生成虫の染色にお
いて色差が小さく、特に核が緑色に発光しないので明白
に区別し難く、かつゴミ等がともに染色されて誤診され
ることが多くあフた。For this reason, the microscope used is expensive, and since Giemser staining is used, there is little color difference between staining blood and schistosome parasites.In particular, the nucleus does not emit green light, so it is difficult to clearly distinguish it, and dirt and other substances are stained together. The condition is often misdiagnosed and left untreated.
このため住血性寄生成虫類の多い地域特に開発途上国に
おける検査が進展せず、これに伴い対策処置が遭まない
という問題点があった。For this reason, there has been a problem that no progress has been made in testing in areas where schistosocytic parasites are prevalent, particularly in developing countries, and as a result, no countermeasures have been taken.
本発明は簡易な装置と方法で正確なＩｌ！！検査を行え
ることを目的とする。The present invention provides accurate Il! using a simple device and method. ! The purpose is to be able to perform inspections.
本発明は上記目的を達成するためになし六もので、検体
を１！祭するために照射するハロゲン光源と、このハロ
ゲン光源より−の光を集光させる集光レンズ及びこの集
光レンズを経た光を所望角に反射せしめる全反射ミラー
と、この集光反射された光線のうち特定波長のみを通す
照射用干渉フィルターと、資料台と、対物レンズと、資
料台、対物レンズを経た光のうち特定波長のみを通す検
出用干渉フィルターと、接眼レンズとを組み合わせて成
る装置を用い、住血性寄生成虫に特定の蛍光色素で染色
し、これを特定波長のみを通す干渉フィルターを透過し
た光で照射し、励起された特定波長以上の光を通す干渉
フィルターを通して顕微鏡下でＩ！察することを要旨と
する。The present invention has six features to achieve the above-mentioned objects, and uses only one sample! A halogen light source used for irradiation, a condensing lens that condenses the light from the halogen light source, a total reflection mirror that reflects the light that has passed through the condensing lens at a desired angle, and the condensed and reflected light rays. A device that combines an irradiation interference filter that passes only a specific wavelength of the light, a document stand, an objective lens, a detection interference filter that passes only a specific wavelength of the light that has passed through the document stand and the objective lens, and an eyepiece. Using a method, a schistosome parasite is dyed with a specific fluorescent dye, irradiated with light that has passed through an interference filter that passes only specific wavelengths, and then exposed under a microscope through an excited interference filter that passes light of a specific wavelength or higher. ! The main point is to understand.
光源にハロゲンランプを用いているので安価で操作性が
よく、しかも染色用の特定蛍光色素として４３０１■〜
５００ｎ＠の光で励起し、５００ｎｍ以上の蛍光を発す
るものを使用するため寄生虫の細胞質が赤色に、核が緑
色に染色され、検体の輪郭が明確となり、さらに核の存
在により寄生虫の判別が容易になり診断に誤りがなくな
る。Since a halogen lamp is used as the light source, it is inexpensive and easy to operate, and 4301■ ~ is used as a specific fluorescent dye for dyeing.
Because it is excited by 500n@ light and uses a substance that emits fluorescence of 500nm or more, the cytoplasm of the parasite is stained red and the nucleus green, making the outline of the specimen clear and further identifying the parasite by the presence of the nucleus. This makes diagnosis easier and eliminates errors in diagnosis.
しかも照射用干渉フィルターの特定波長を４４０〜５０
０ｎｖｉ、検出用干渉フィルターの特定波長を５２０ｎ
ｗ以上の波長を透過するものを使用しているので、４４
０〜５００ｇｍの特定波長で寄生虫の細胞質が赤色に、
核が緑色に励起されるため、５２０ｎ■以上の波長で観
察することＣ；より検査対象が明確に区別され、低倍率
の顕微鏡でも正確な検査を行えるとともにコンデンサー
レンズが不要となり装置が安価なものでよい。Moreover, the specific wavelength of the interference filter for irradiation is 440 to 50.
0nvi, the specific wavelength of the detection interference filter is 520n
Since we are using a material that transmits wavelengths of W or more, 44
The cytoplasm of the parasite turns red at a specific wavelength of 0 to 500gm,
Because the nucleus is excited in green, it should be observed at a wavelength of 520n or higher; the object to be inspected can be more clearly distinguished, accurate inspection can be performed even with a low-magnification microscope, and a condenser lens is not required, making the device inexpensive. That's fine.
以下本発明を図示の実施例にもとづいて説明する。 The present invention will be explained below based on the illustrated embodiments.
第１図は本発明のＩＩ寮装置の概略を示す説明図で、
１は安価で−作性のよいハロゲンランプで、このハロゲ
ンランプ１を光源として用い、２はこのハロゲンランプ
１に近接設置される集光レンズで、ハロゲンランプ１よ
りの光を集光させ、全反射ミラー３にて所望方向へ屈折
させ、この屈折された光はこの上方に配設された干渉フ
ィルター４を透過されるようになす。この照射用干渉フ
ィルター４は４３０〜５（１（ｌｎｍの特定波長のみを
通する干渉フィルターを使用する。FIG. 1 is an explanatory diagram showing the outline of the II dormitory device of the present invention,
Reference numeral 1 denotes a halogen lamp that is inexpensive and has good productivity. This halogen lamp 1 is used as a light source, and 2 is a condenser lens installed close to the halogen lamp 1, which condenses the light from the halogen lamp 1 and collects the entire light. The light is refracted in a desired direction by a reflecting mirror 3, and the refracted light is transmitted through an interference filter 4 disposed above. This irradiation interference filter 4 uses an interference filter that passes only a specific wavelength of 430 to 5 (1 nm).
この照射用干渉フィルター４の上方には資料台５があり
、資料台上には検体６例えば住血性寄生成虫・マラリア
原虫等が載置される。この検体は正確なＷ＆寮検査を行
えるよう特定の蛍光色素にて染色されている。この蛍光
色素として検査対象となる血液を４３０〜５００ｎｍの
光で励起し、５０Ｄｎｍ以上の蛍光を発する色素例えば
アクリデイン、オレンジコリフォスフイン等の単染色ま
たはクイナラランとプロビチュームアイオダイド、エチ
チュームイアオダイド等の二重染色により染色されてい
る。Above this irradiation interference filter 4 is a data table 5, on which a specimen 6, such as schistosocytic parasites, malaria parasites, etc., is placed. This specimen is stained with a specific fluorescent dye to enable accurate W&D testing. As this fluorescent dye, the blood to be tested is excited with light of 430 to 500 nm, and dyes that emit fluorescence of 50 Dnm or more are used for single staining, such as acrideine, orange corifosphin, or Quinararan, probitum iodide, ethytum iodide, etc. stained by double staining.
この資料台上方には対物レンズ７と検出用干渉用フィル
ター８、接眼レンズ９が配置され、接眼レンズ９より資
料台上の検体を観察検査するもので、上記検出用干渉用
フィルターとして５２０ｎｍ以上の特定波長を通す干渉
フィルターが用いられる。An objective lens 7, a detection interference filter 8, and an eyepiece 9 are arranged above this data table, and the specimen on the data table is observed and inspected through the eyepiece 9. An interference filter is used that passes specific wavelengths.
本発明住血性寄生成虫の観察方法及びその装置によれば
、検体を４３０〜５ＱＯｎｉの光で励起し５００１−以
上の蛍光を発する色素でもって染色をし、これをハロゲ
ンランプにて特定波長を通す照射用干渉フィルター、及
び特定波長を通す検出用干渉フィルターを通して検査す
るようになしているため、特定波長で寄生虫の細胞質が
赤色に、核が緑色に励起され、検査対象の輪郭が明確に
なり低倍率の安価な顕微鏡を用いても、判別が容易とな
り診断が正確に迅速に行える利点を有する。According to the method and device for observing schistophematous parasites of the present invention, a specimen is excited with light of 430 to 5 QOni, stained with a dye that emits fluorescence of 5001 or more, and then passed through a specific wavelength using a halogen lamp. Since the inspection is performed through an interference filter for irradiation and an interference filter for detection that passes specific wavelengths, the cytoplasm of the parasite is excited in red and the nucleus in green at specific wavelengths, making the outline of the inspection target clear. Even if an inexpensive microscope with low magnification is used, it has the advantage that discrimination can be made easily and diagnosis can be made accurately and quickly.
第１図は本発明住血性寄生成虫の観察方法及びその装置
の一実施例を示す概略の説明図である。
ｌはハロゲンランプ、４は照射用干渉フィルター、５は
資料台、６は検体、８は検出用干渉フィルター、 ９は
接眼レンズ。FIG. 1 is a schematic explanatory diagram showing one embodiment of the method and apparatus for observing schistosocytic parasites of the present invention. l is a halogen lamp, 4 is an interference filter for irradiation, 5 is a data table, 6 is a specimen, 8 is an interference filter for detection, and 9 is an eyepiece.
Claims (5)
を特定波長のみを通す干渉フィルターを透過した光で照
射し、励起された特定波長以上の光を通す干渉フィルタ
ーを通して顕微鏡下で観察することを特徴とする住血性
寄生成虫の観察方法。(1) Stain the schistosome parasite with a specific fluorescent dye, irradiate it with light that has passed through an interference filter that passes only specific wavelengths, and observe it under a microscope through the excited interference filter that passes light of a specific wavelength or higher. A method for observing schistophematous parasites.
００ｎｍ、検出用の干渉フィルターの特定波長を５２０
ｎｍ以上の波長を透過するものとする請求項（１）記載
の住血性寄生成虫の観察方法。(2) Set the specific wavelength of the interference filter for irradiation to 430-5
00nm, and the specific wavelength of the interference filter for detection is 520nm.
2. The method for observing schistocytic parasites according to claim 1, which transmits wavelengths of nm or more.
ｍの光で励起し、５００ｎｍ以上の蛍光を発する色素を
使用する請求項（１）記載の住血性寄生成虫の観察方法
。(3) 440nm to 500n for specific fluorescent dyes for staining
2. The method for observing schistocytic parasites according to claim 1, wherein a dye that is excited by light of m and emits fluorescence of 500 nm or more is used.
載の住血性寄生成虫の観察方法。(4) The method for observing a schistotic parasite according to claim (1), wherein the schistotic parasite is malaria.
このハロゲン光源よりの光を集光させる集光レンズ及び
この集光レンズを経た光を所望角に反射せしめる全反射
ミラーと、この集光反射された光線のうち特定波長のみ
を通す照射用干渉フィルターと、資料台と、対物レンズ
と、資料台、対物レンズを経た光のうち特定波長のみを
通す検出用干渉フィルターと、接眼レンズとを組み合わ
せて成ることを特徴とする住血性寄生成虫の観察装置。(5) a halogen light source that irradiates to observe the specimen;
A condensing lens that condenses the light from the halogen light source, a total reflection mirror that reflects the light that has passed through the condensing lens at a desired angle, and an irradiation interference filter that passes only a specific wavelength of the condensed and reflected light. , a document table, an objective lens, a detection interference filter that allows only a specific wavelength of light to pass through the document table and the objective lens, and an eyepiece. .
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31913890A JPH04188061A (en) | 1990-11-22 | 1990-11-22 | Method and apparatus for observing hematobium parasite adult |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31913890A JPH04188061A (en) | 1990-11-22 | 1990-11-22 | Method and apparatus for observing hematobium parasite adult |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04188061A true JPH04188061A (en) | 1992-07-06 |
Family
ID=18106875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP31913890A Pending JPH04188061A (en) | 1990-11-22 | 1990-11-22 | Method and apparatus for observing hematobium parasite adult |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH04188061A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8081312B2 (en) | 1997-05-05 | 2011-12-20 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
-
1990
- 1990-11-22 JP JP31913890A patent/JPH04188061A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8081312B2 (en) | 1997-05-05 | 2011-12-20 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
| US8125643B2 (en) | 1997-05-05 | 2012-02-28 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
| US8363221B2 (en) | 1997-05-05 | 2013-01-29 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
| US8432550B2 (en) | 1997-05-05 | 2013-04-30 | Chemometec A/S | Method and a system for determination of particles in a liquid sample |
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