JPH04187081A - Method for storing va mycorrhiza - Google Patents
Method for storing va mycorrhizaInfo
- Publication number
- JPH04187081A JPH04187081A JP31709590A JP31709590A JPH04187081A JP H04187081 A JPH04187081 A JP H04187081A JP 31709590 A JP31709590 A JP 31709590A JP 31709590 A JP31709590 A JP 31709590A JP H04187081 A JPH04187081 A JP H04187081A
- Authority
- JP
- Japan
- Prior art keywords
- spores
- carrier
- zeolite
- germination rate
- stored
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 14
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 17
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000010457 zeolite Substances 0.000 claims abstract description 17
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 239000002245 particle Substances 0.000 claims abstract description 7
- 241000233866 Fungi Species 0.000 claims description 21
- 238000004321 preservation Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 230000007774 longterm Effects 0.000 abstract description 3
- 230000035784 germination Effects 0.000 description 23
- 239000002689 soil Substances 0.000 description 12
- 241000196324 Embryophyta Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 4
- 239000000969 carrier Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 230000004763 spore germination Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000010455 vermiculite Substances 0.000 description 2
- 229910052902 vermiculite Inorganic materials 0.000 description 2
- 235000019354 vermiculite Nutrition 0.000 description 2
- 206010016807 Fluid retention Diseases 0.000 description 1
- 241000235500 Gigaspora Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 241001595840 Margarites Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000012773 agricultural material Substances 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000008262 pumice Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、ＶＡ菌根菌の胞子を保存する方法に関するも
のである。DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a method for preserving spores of VA mycorrhizal fungi.
ＶＡ菌根菌は植物と共生させることにより植物の成育を
旺盛にさせ、土壌病害の発病を抑制軽減させることがで
きるものである。By coexisting with plants, VA mycorrhizal fungi can promote vigorous plant growth and suppress and reduce the onset of soil diseases.
（従来の技術）
ＶＡ菌根菌はリンやミネラル等の微量成分を土壌から吸
収して宿主植物に供給することから生物肥料としての役
割が注目され、作物の成育促進、病害抑制などに有効で
あることから、新しい農業資材として有望視されている
。(Conventional technology) VA mycorrhizal fungi have attracted attention for their role as biofertilizers, as they absorb trace elements such as phosphorus and minerals from the soil and supply them to host plants, and are effective in promoting crop growth and suppressing diseases. For this reason, it is seen as a promising new agricultural material.
かかるＶＡ菌根菌の保存については一般には次のような
方法が用いられている。（１）宿根性植物の根にＶＡ菌
根菌を共生させた状態（菌根）で植物を栽培して保存し
たり、（２） Ｖ Ａ菌根菌が共生した植物の栽培終了
後の跡地土壌を冷蔵庫（０℃〜１０℃）で保管したり、
あるいは（３）跡地土壌より分離したＶＡ菌根菌の胞子
の表面を殺菌し、０℃〜１０℃に保たれ殺菌水中で保管
している。The following methods are generally used to preserve such VA mycorrhizal fungi. (1) Cultivation and preservation of plants with VA mycorrhizal fungi coexisting with the roots of perennial plants (mycorrhizae), and (2) Sites left behind after cultivation of plants with VA mycorrhizal fungi coexisting symbiotically. Store the soil in the refrigerator (0°C to 10°C),
Alternatively, (3) the surface of VA mycorrhizal fungus spores separated from the site soil is sterilized and stored in sterilized water at a temperature of 0°C to 10°C.
（発明が解決しようとする問題点）
従来の方法では、植物を栽培して保存するためには広大
な施設を必要とし、しかも植物栽培が長期に亘る合は栽
培中に雑菌による汚染の恐れがあること。跡地土壌をそ
のまま保存するためには低温で保管する必要がありエネ
ルギーコストが高いこと。また、胞子を分離して表面殺
菌する方法は操作が煩雑であり、完全に殺菌することが
困難なため保管中に雑菌による汚染の恐れがある。その
ため、定期的に殺菌を繰り返す必要があり、しかも低温
で保管しなければならないのでエネルギーコストが高い
。そのため以上に述べた方法では、少量のＶＡ菌根菌の
保存には適しているが、大量のＶＡ菌根菌を保存する方
法としては不向きである。(Problems to be solved by the invention) Conventional methods require vast facilities to cultivate and preserve plants, and when plants are cultivated for a long period of time, there is a risk of contamination by bacteria during cultivation. Something. In order to preserve the soil at the site, it must be stored at low temperatures, which requires high energy costs. In addition, the method of separating spores and sterilizing the surface requires complicated operations and is difficult to completely sterilize, so there is a risk of contamination with various bacteria during storage. Therefore, it is necessary to repeat sterilization on a regular basis, and it also has to be stored at low temperatures, resulting in high energy costs. Therefore, the method described above is suitable for preserving a small amount of VA mycorrhizal fungi, but is not suitable for preserving a large amount of VA mycorrhizal fungi.
（問題点を解決するための手段）
本発明は上記の諸問題を解決するためになされたもので
、ＶＡ菌根菌を保存する方法において、その胞子の担体
としてゼオライト混合物を用い、かつ担体の水分量を調
節することにより常温下でのＶＡ菌根菌の胞子を長期間
安定的に保存することを可能としたものである。(Means for Solving the Problems) The present invention has been made to solve the above-mentioned problems, and is a method for preserving VA mycorrhizal fungi, in which a zeolite mixture is used as a carrier for the spores, and a zeolite mixture is used as a carrier for the spores. By adjusting the moisture content, it is possible to store VA mycorrhizal fungus spores stably for a long period of time at room temperature.
以下本発明の方法を詳細に説明する。The method of the present invention will be explained in detail below.
ＶＡ菌根菌の収集は、−船釣な方法として行われている
胞子を含有した土壌を目開き２ＩＩ１ｍｌの篩に通し異
物や大きな土壌粒子を除去する。Collection of VA mycorrhizal fungi is carried out by boat fishing, in which soil containing spores is passed through a 2II 1 ml sieve to remove foreign matter and large soil particles.
ついで、ウェット・シーヴイング法等の常法を用いて胞
子を分離し、保存用担体と混合し密閉容器に充填するも
のである。The spores are then separated using a conventional method such as wet sieving, mixed with a storage carrier, and filled into a sealed container.
保存用担体としては、赤土、火山灰土、沖積土、砂、軽
石、パーライト、粉炭、ピートモスおよびバーミキュラ
イト等の園芸用培土とゼオライトとの混合物からなり、
ゼオライトを２〜５０％（容量比）、好ましくは５〜３
０％含有している担体を使用するものである。使用する
ゼオライトは特に限定するものではな（、通常は粒径５
Ｉｌｌｆｆｉ以下のものが好適に用いられる。ここで、
ゼオライトの配合がもたらす効果は明らかではないが、
保存時担体の保水に関与するものと考えられ、その配合
量が２％以下ではそれ相応の効果が乏しく、また５０％
以上では発芽に長時間を要するので好ましくない。Preservation carriers include mixtures of zeolite and horticultural soil such as red soil, volcanic ash soil, alluvial soil, sand, pumice, perlite, pulverized charcoal, peat moss, and vermiculite.
Zeolite at 2-50% (volume ratio), preferably 5-3
A carrier containing 0% is used. There are no particular restrictions on the zeolite used (the particle size is usually 5.
Illffi or less is preferably used. here,
Although the effect of zeolite combination is not clear,
It is thought to be involved in the water retention of the carrier during storage, and if the amount is less than 2%, the corresponding effect will be poor, and if the amount is less than 2%,
This is not preferable because it takes a long time for germination.
なお、保存用担体とＶＡ菌根菌の胞子を混合する前に、
予め保存用担体の含有水分を１０〜３０％、好ましくは
２０％付近に調節しておくことが長期保存に好適である
。In addition, before mixing the storage carrier and VA mycorrhizal fungus spores,
It is suitable for long-term storage to adjust the moisture content of the storage carrier in advance to 10 to 30%, preferably around 20%.
水分量が、１０％以下では保存時の乾燥が影響し胞子の
劣化が起こり発芽率が低下する。また、３０％以上にな
ると保存中に胞子が発芽するため好ましくない。If the moisture content is less than 10%, the spores will deteriorate due to dryness during storage and the germination rate will decrease. Moreover, if it exceeds 30%, spores will germinate during storage, which is not preferable.
なお、担体に対するＶＡ菌根菌胞子の混合量は特に規制
はないが、単体ｇｒ当たり５〜１０００個程度である。The amount of VA mycorrhizal fungus spores mixed with the carrier is not particularly regulated, but is about 5 to 1000 per single gr.
以下、実施例および比較例により本発明を説明するが、
これらによって限定されるものではない。The present invention will be explained below with reference to Examples and Comparative Examples.
It is not limited to these.
実施例１
大豆を栽培した跡地土壌よりウェット・シーヴイング法
でＶＡ菌根菌（Ｇｉｇａｓｐｏｒａ Ｍａｒｇａｒｉｔ
ａ）の胞子を分離した。ついで分離した胞子の発芽率を
調べるため、胞子を２％クロラミンＴ水溶岐、および２
００ｐｐｍストレプトマイシン水溶故に各１０分間浸漬
し表面殺菌を行った。Example 1 VA mycorrhizal fungi (Gigaspora Margarit
The spores of a) were isolated. Next, in order to examine the germination rate of the isolated spores, the spores were dissolved in 2% chloramine T water and 2% chloramine T.
00 ppm streptomycin was dissolved in water, so the surface was sterilized by immersion for 10 minutes each.
しかるのち、滅菌水で水洗後、寒天（デイフコ社製バク
トアガー）の平板上に殺菌法の胞子を１２粒ずつのせ、
２５℃インキュペターで培養した。After that, after washing with sterile water, 12 spores of the sterilization method were placed on a plate of agar (Bacto Agar manufactured by Difco).
The cells were cultured in an incubator at 25°C.
培養１週間後、２週間後および３週間後の胞子の発芽率
を第１表に示す。胞子の状態は良好で、２週間で約８０
％の胞子が発芽した。Table 1 shows the germination rates of spores after 1 week, 2 weeks, and 3 weeks of culture. The spores are in good condition, about 80 in 2 weeks.
% of spores germinated.
第 １ 表
実施例２
実施例工と同様に大豆を栽培した跡地より分離したＶＡ
菌根菌の胞子を保存するため保存用担体と混合し、密閉
容器に充填し５℃の恒温器中で２力月保管した。Table 1 Example 2 VA separated from the site where soybeans were cultivated in the same way as in the example work
In order to preserve the spores of mycorrhizal fungi, they were mixed with a preservation carrier, filled into a sealed container, and stored in a thermostat at 5° C. for 2 months.
保存用担体としては、粒径２ＩＩＩＩ＋以下の赤土に同
じ＜２ａｍ以下のゼオライト（日東粉化工業■）を０％
、１０％、２０％、５０％（容量比）で混合し、含有水
分を７％、１５％、２０％、３０％、４５％となるよう
に調整したものを用いた。なお、ＶＡ菌根菌胞子は担体
ｇｒ当たり１００個の割合で混合したものを用い恒温器
中で２ヵ月保管した後、密閉容器中の保存用担体から胞
子を分離し、実施例１に準じた方法で胞子の発芽率を調
べた。第２表に培養２週間後の胞子の発芽率を示す。As a storage carrier, 0% zeolite (Nitto Funka Kogyo ■) with the same particle size of <2 am or less is added to red clay with a particle size of 2III+ or less.
, 10%, 20%, and 50% (volume ratio), and the water content was adjusted to 7%, 15%, 20%, 30%, and 45%. In addition, VA mycorrhizal fungal spores were mixed at a ratio of 100 per carrier gr and stored in a thermostatic chamber for 2 months, after which the spores were separated from the storage carrier in a sealed container and prepared according to Example 1. The germination rate of spores was investigated using the method. Table 2 shows the germination rate of spores after 2 weeks of culture.
第 ２ 表
第２表からも判るように、低温下（５℃）での保存では
水分７％のときは胞子の劣化が認められたが、１５％以
上では胞子の状態は良好であり発芽率も安定している。Table 2 As can be seen from Table 2, when stored at low temperatures (5°C), spores deteriorated when the moisture content was 7%, but when the moisture content was 15% or higher, the spores were in good condition and the germination rate was low. is also stable.
また、ゼオライトの含有量が１０％〜２０％のものは発
芽率も優れており、低温保存であれば特に問題はない。In addition, those with a zeolite content of 10% to 20% have an excellent germination rate, and there are no particular problems as long as they are stored at low temperatures.
実施例３
実施例２と同様な方法で調整したＶＡ菌根菌の胞子入り
担体を密閉容器に充填し、２５℃の恒温器中で２ケ月間
保管し、実施例１に準じた方法で胞子の発芽率を調べた
。第３表に培養２週間後の発芽率を示す。Example 3 A carrier containing spores of VA mycorrhizal fungi prepared in the same manner as in Example 2 was filled into a sealed container, stored in a thermostat at 25°C for 2 months, and spore-containing was prepared in the same manner as in Example 1. The germination rate was investigated. Table 3 shows the germination rate after 2 weeks of culture.
第 ３ 表
第３表からもわかるように、含有水分７％のときは大半
の胞子が劣化していた。また、１５％では一部の胞子に
劣化が認められたが発芽は安定していた。２０％では胞
子の状態は最も良好であり、発芽率も安定していた。ま
た、３０％以上では保管中に発芽がおこり保存には不適
当であった。Table 3 As can be seen from Table 3, most of the spores were degraded when the moisture content was 7%. In addition, at 15%, some spores were observed to be degraded, but germination was stable. At 20%, the spores were in the best condition and the germination rate was also stable. Moreover, if the content was 30% or more, germination occurred during storage, making it unsuitable for preservation.
また、ゼオライトの添加により胞子の表面は汚れが少な
くなり好ましいが、含有量が５０％になると発芽率がや
や低下する。Furthermore, the addition of zeolite is preferable because it reduces the amount of dirt on the surface of the spores, but when the content reaches 50%, the germination rate decreases slightly.
実施例４
実施例２と同様な方法で調整したＶＡ菌根菌の胞子を担
体（水分含有量７％、２０％、４５％、ゼオライト含有
量２０％）と共にを密閉容器に充填し、５℃および２５
℃の恒温器中で８力月間保管した。保管中定期的に胞子
をサンプリングし、実施例２に準じた方法で発芽率を調
べた。第４表および第５表に各温度における培養２週間
後の発芽率を示す。Example 4 Spores of VA mycorrhizal fungi prepared in the same manner as in Example 2 were filled into a sealed container together with a carrier (moisture content: 7%, 20%, 45%, zeolite content: 20%) and heated at 5°C. and 25
It was stored in a thermostat at ℃ for 8 months. Spores were sampled periodically during storage, and the germination rate was examined in the same manner as in Example 2. Tables 4 and 5 show the germination rates after two weeks of culture at each temperature.
第４表 保管温度５℃における胞子の発芽率第５表 保
管温度２５℃における胞子の発芽率第４表および第５表
より５℃で保管した場合含有水分量７％以外は８力月後
も胞子の状態が良好で発芽率も安定していた。一方、２
５°Ｃで保管した場合、含有水分が大略２０％の場合は
胞子の状態は良好で且つ、発芽率も安定しており、５℃
で保管した場合と同様の発芽率をしめしていることが判
った。Table 4 Spore germination rate at a storage temperature of 5°C Table 5 Spore germination rate at a storage temperature of 25°C From Tables 4 and 5, when stored at 5°C, moisture content other than 7% will persist after 8 months. The spores were in good condition and the germination rate was stable. On the other hand, 2
When stored at 5°C, the spores are in good condition and the germination rate is stable when the moisture content is approximately 20%.
It was found that the germination rate was the same as when stored in
比較例１および２
実施例２と同様な方法で調整したＶＡ菌根菌の胞子を、
粒径２ＩＩＩＩ１１以下の赤土にバーミキュライト２０
％（容量比）混合したもの（比較例１）および赤土に川
砂２０％（容量比）混合したもの（比較例２）を含有水
分が２０％となるように調整し密閉容器に充填したのち
、２５℃の恒温器中で８力月間保管した。保管中に定期
的に胞子をサンプリングし、実施例１に準じた方法で発
芽率を調べた。Comparative Examples 1 and 2 VA mycorrhizal fungus spores prepared in the same manner as in Example 2 were
Vermiculite 20 on red soil with particle size 2III11 or less
% (volume ratio) mixture (Comparative Example 1) and red clay mixed with 20% (volume ratio) river sand (Comparative Example 2) were adjusted to have a moisture content of 20% and filled into a sealed container. It was stored in a thermostat at 25°C for 8 months. Spores were sampled periodically during storage, and the germination rate was examined in the same manner as in Example 1.
第６表に培養２週間後の発芽率を示す。Table 6 shows the germination rate after 2 weeks of culture.
第６表 （保管温度２５℃における胞子の発芽率）第６
表から判るようにゼオライトを含有しない担体は、これ
を含有する担体と比べ発芽率に可成りの差異が認められ
る。Table 6 (Germination rate of spores at storage temperature 25℃) No. 6
As can be seen from the table, there is a considerable difference in germination rate between carriers containing no zeolite and carriers containing zeolite.
（発明の効果）
本発明の方法によれば、簡便な方法でＶＡ菌根菌胞子の
常温下における長期保存が可能である。(Effects of the Invention) According to the method of the present invention, long-term storage of VA mycorrhizal fungal spores at room temperature is possible using a simple method.
Claims (3)
としてゼオライト混合物を用いることを特徴とするＶＡ
菌根菌の保存方法。(1) VA characterized in that a zeolite mixture is used as a carrier for preserving spores of VA mycorrhizal fungi.
How to preserve mycorrhizal fungi.
とを特徴とする請求項１記載の保存方法。(2) The preservation method according to claim 1, characterized in that the moisture content of the carrier is 30% or less and up to 10%.
容量比）以下２％まで混合することを特徴とする請求項
１および２記載の保存方法。(3) Zeolite with a particle size of 5 mm or less accounts for 50% of the carrier (
3. The preservation method according to claim 1, characterized in that the mixture is mixed up to a volume ratio of 2% or less.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2317095A JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2317095A JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH04187081A true JPH04187081A (en) | 1992-07-03 |
| JPH0757180B2 JPH0757180B2 (en) | 1995-06-21 |
Family
ID=18084384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2317095A Expired - Fee Related JPH0757180B2 (en) | 1990-11-21 | 1990-11-21 | Method for storing VA mycorrhizal fungus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0757180B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06189745A (en) * | 1992-12-25 | 1994-07-12 | Central Glass Co Ltd | Microorganism material |
| WO1994016055A1 (en) * | 1993-01-07 | 1994-07-21 | Idemitsu Kosan Company Limited | Method of preserving spore of va mycorrhizal fungus |
| CN108315261A (en) * | 2018-04-13 | 2018-07-24 | 四川省农业科学院土壤肥料研究所 | A kind of edible mushroom storage medium and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03128988A (en) * | 1989-07-28 | 1991-05-31 | Tochigi Pref Gov | Microbial material capable of controlling soil disease and its manufacture |
-
1990
- 1990-11-21 JP JP2317095A patent/JPH0757180B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03128988A (en) * | 1989-07-28 | 1991-05-31 | Tochigi Pref Gov | Microbial material capable of controlling soil disease and its manufacture |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06189745A (en) * | 1992-12-25 | 1994-07-12 | Central Glass Co Ltd | Microorganism material |
| WO1994016055A1 (en) * | 1993-01-07 | 1994-07-21 | Idemitsu Kosan Company Limited | Method of preserving spore of va mycorrhizal fungus |
| CN108315261A (en) * | 2018-04-13 | 2018-07-24 | 四川省农业科学院土壤肥料研究所 | A kind of edible mushroom storage medium and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0757180B2 (en) | 1995-06-21 |
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