JPH04169165A - Production of seasoning - Google Patents

Production of seasoning

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Publication number
JPH04169165A
JPH04169165A JP2295408A JP29540890A JPH04169165A JP H04169165 A JPH04169165 A JP H04169165A JP 2295408 A JP2295408 A JP 2295408A JP 29540890 A JP29540890 A JP 29540890A JP H04169165 A JPH04169165 A JP H04169165A
Authority
JP
Japan
Prior art keywords
seasoning
lipase
roasted
protease
raw material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2295408A
Other languages
Japanese (ja)
Inventor
Youichi Itohiya
陽一 糸日谷
Taku Kijima
木島 卓
Hikotaka Hashimoto
橋本 彦尭
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kikkoman Corp
Original Assignee
Kikkoman Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corp filed Critical Kikkoman Corp
Priority to JP2295408A priority Critical patent/JPH04169165A/en
Publication of JPH04169165A publication Critical patent/JPH04169165A/en
Pending legal-status Critical Current

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  • Seasonings (AREA)

Abstract

PURPOSE:To obtain a seasoning excellent in fanciness by hydrolysis of a roasted and dried product of fish and shellfish as raw material using two kinds of enzyme, protease and lipase. CONSTITUTION:The objective seasoning can be obtained by hydrolysis of a roasted and dried product of fish and shellfish (e.g. bonito) as raw material using two kinds of enzyme, (A) 20-3500 (pref. 400-2000) units per g weight of protein of protease and (B) 100-7200 (pref. 350-1800) units per g weight of lipid of lipase.

Description

【発明の詳細な説明】 本発明は、安価な魚介類原料を用いて、天然鰹節のフレ
ーバーを濃厚に有し、しかも透明な調味料を製造する方
法に関する。
DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing a transparent seasoning that has a rich flavor of natural bonito flakes using inexpensive seafood raw materials.

従来生または、煮熟後乾燥した、魚介類原料をプロテア
ーゼにより加水分解して調味料を製造することか、いく
つか知られているか、何れの場合も原料特有の生臭味を
有し、嗜好的に充分満足するものではなかった。
There are several known methods of producing seasonings by hydrolyzing raw or boiled and dried seafood raw materials with protease, and in either case, the raw materials have a fishy odor and taste that is appealing to the taste. It was not completely satisfying.

また、製品には程度の差はあるか、濁りを有し、しかも
この濁りは非常に微細なため通常の濾布濾過等では取除
くことか困難である。そのため従来は、高濃度の珪藻土
添加と硅藻土慮過に伴う風味の減少を余儀なくされる欠
点を有していた。
In addition, the products have turbidity to varying degrees, and this turbidity is so minute that it is difficult to remove it by ordinary filter cloth filtration. For this reason, conventional products have had the disadvantage of being forced to add a high concentration of diatomaceous earth and to reduce the flavor due to the excessive use of diatomaceous earth.

そこで、本発明者らは、このような現状に鑑み、これら
の魚介類を原料として用い、生臭味のない、嗜好的に風
味の良好で、鰹節のフレーバーを有し、透明なタイプの
調味料を、珪藻土などの慮過助剤を高濃度に用いること
なく得ることを目的として、種々検討を重ねた結果、原
料として焙乾処理した魚介類を用い、これをプロテアー
ゼとリパーゼにより酵素分解をすることにより上記目的
か達成されることを知り、この知見に基づいて本発明を
完成した。
Therefore, in view of the current situation, the present inventors have developed a transparent type of seasoning that does not have fishy odor, has a pleasant taste, has the flavor of bonito flakes, and uses these seafood as raw materials. As a result of various studies, we used roasted seafood as the raw material and enzymatically decomposed it with protease and lipase. The present invention was completed based on this knowledge.

即ち、本発明は魚介類原料を酵素により加水分解して調
味料を製造するに当り、該原料として魚分類の焙乾処理
物を用い、また酵素としてプロテアーゼ及びリパーゼを
併用添加することを特徴とする調味料の製造法である。
That is, the present invention is characterized in that when producing seasonings by hydrolyzing seafood raw materials with enzymes, a roasted and dried product of fish classification is used as the raw material, and protease and lipase are added together as enzymes. This is a method for producing seasonings.

以下、本発明の詳細な説明する。The present invention will be explained in detail below.

先ず、本発明に用いられる魚介類原料としてはカツオ、
サバ、イワシ等の魚類、イカ、タコ等の頭足類、アサリ
、ハマグリ等の貝類等が挙げられる。これらは、何れの
部位も使用可能であるが、缶詰めや、節類製造の際に副
生ずる頭部や、腹内部も利用可能である。特に、鰹節製
造の際に副生ずるカツオの腹内の部分「ハラモ」は、後
で述べる焙乾処理に依って天然鰹節風味を濃厚に有する
調味料が得られるので好ましい。
First, the seafood raw materials used in the present invention include bonito,
Examples include fish such as mackerel and sardines, cephalopods such as squid and octopus, and shellfish such as clams and clams. Any part of these can be used, but the head, which is a by-product during canning or joint production, and the inside of the abdomen can also be used. Particularly, the inner part of the bonito belly "Haramo" which is produced as a by-product during the production of bonito flakes is preferable because a seasoning with a rich natural bonito flavor can be obtained by roasting and drying, which will be described later.

本発明はこのような魚介類原料の焙乾処理物を用いるこ
とを特徴としている。このことは、極めて重要で、焙乾
処理をしていない原料では、原料の生臭味が最終製品に
まで移行し、風味の良好な調味料が得られない。
The present invention is characterized by using a roasted and dried product of such seafood raw materials. This is extremely important; if raw materials are not roasted and dried, the raw odor of the raw materials will be transferred to the final product, making it impossible to obtain seasonings with good flavor.

焙乾処理の条件としては、上記原料を加熱又は煮熟変性
したものを、■そのまま乾燥を兼ねて常法により焙乾処
理するか、又は■乾燥した後焙乾処理する。上記焙乾は
原料を薫煙室内で75〜120℃の、餅材の余煙に曝し
、水分を25〜35%とする。
The conditions for the roasting treatment are as follows: (1) The above-mentioned raw material is heated or boiled and denatured, and then either (1) it is directly roasted and then dried by a conventional method, or (2) it is dried and then roasted. In the above-mentioned roasting process, the raw material is exposed to the residual smoke of rice cake material at 75 to 120°C in a smoke room, and the moisture content is adjusted to 25 to 35%.

次に、酵素による加水分解は、上記焙乾処理した原料を
予め必要により適当な粒度に割砕したものを、1〜20
(重量)倍の水と混合し、70〜120’Cで、1〜3
0分煮熟した後室温迄冷却し、これにプロテアーゼ及び
リパーゼをそれぞれ所定量併用添加する。
Next, for enzymatic hydrolysis, the above-mentioned roasted and dried raw material is crushed into suitable particle sizes as required, and the
(weight) mix with water and heat at 70-120'C for 1-3 hours.
After boiling for 0 minutes, the mixture is cooled to room temperature, and predetermined amounts of protease and lipase are added thereto.

即ち、プロテアーゼを原料中の蛋白質g重量当り20〜
3500単位、特に400〜2000単位を添加し、ま
たリパーゼを同原料中の脂質g重量当り100〜720
0、特に350〜1800単位添加する。
That is, the protease is added at a concentration of 20 to
3500 units, especially 400-2000 units, and lipase at 100-720 units per g weight of lipid in the same raw material.
0, especially 350 to 1800 units.

なお、プロテアーゼの活性は、該酵素をカゼインに作用
させるとき、1分間に1μgのチロシンに相当する非蛋
白性のフォリン試液呈色物質を生成する酵素力を1単位
とする。また、リパーゼの活性は、日本油化学協会編 
基準油脂分析試験法による。
Regarding the activity of protease, one unit is the enzymatic power to produce a non-protein Folin reagent colored substance corresponding to 1 μg of tyrosine per minute when the enzyme is allowed to act on casein. In addition, the activity of lipase is
Based on the standard oil and fat analysis test method.

プロテアーゼ及びリパーゼを併用添加することは本発明
において極めて重要で、何れか一方のみでは風味の良好
な調味料は得られない。またそれぞれの添加量か少なす
ぎても同様に得られない。
It is extremely important in the present invention to add protease and lipase in combination; a seasoning with good flavor cannot be obtained by using either one alone. Moreover, if the amount of each added is too small, the same result cannot be obtained.

更にリパーゼを添加しないときは、透明な調味液は得ら
れない。即ち、分解液中に微細な物質か多量に懸濁浮遊
する。この物質は、通常の濾布慮過では取除くことが困
難なため珪藻土の高濃度の添加を余儀なくされ、またそ
の慮過の際風味か失われる新たな問題か発生する。
Furthermore, when lipase is not added, a clear seasoning liquid cannot be obtained. That is, a large amount of fine substances are suspended in the decomposition solution. Since this substance is difficult to remove by ordinary filter cloth filtering, it is necessary to add diatomaceous earth at a high concentration, and when filtering it, a new problem arises in that flavor is lost.

これに対し、プロテアーゼおよびリパーゼをそれぞれ上
記の範囲で併用添加するときは、風味が頗る濃厚で、し
かも透明な調味料か得られる。
On the other hand, when protease and lipase are added in combination within the above-mentioned ranges, a rich and clear seasoning with a pronounced flavor can be obtained.

上記加水分解の条件は、従来公知の方法をそのまま用い
ることか出来る。例えば、室温〜70℃で、2〜10時
間、特に3〜6時間、必要により(時々)攪拌しながら
分解を行い、その後腐敗防止のため食塩を終り濃度12
〜16%になるように加え、更に同条件下で18〜24
時間加水分解を行う。なおpHを、45〜8.5、特に
56〜75に調製するときは、嗜好的に良好な鰹節のフ
レーバーが得られるので好ましい。
The above-mentioned hydrolysis conditions may be the same as conventionally known methods. For example, decomposition is carried out at room temperature to 70°C for 2 to 10 hours, especially 3 to 6 hours, with stirring if necessary (occasionally), and then salt is added to prevent spoilage and the concentration is 12.
16% and further under the same conditions 18-24%
Perform time hydrolysis. Note that it is preferable to adjust the pH to 45 to 8.5, particularly 56 to 75, because a palatable flavor of bonito flakes can be obtained.

このようにして得られた調味液は常法により加熱処理(
例えば、100〜120℃で15〜30分)した後、濾
布慮過等を行って製品とする。
The seasoning liquid obtained in this way is heat treated (
For example, after heating at 100 to 120° C. for 15 to 30 minutes, a product is obtained by passing through a filter cloth or the like.

このようにして本発明によれば高価な鰹節を原料とする
ことなく、比較的入手が容易な魚介類を原料として、天
然の鰹節のフレーバーを濃厚に有し、アミノ酸特にグル
タミン酸か多くて旨味の濃厚な、しかも透明なタイプの
調味料を、珪藻土などの慮過助剤を用いることなく濾布
慮過等の比較的簡単な慮過手段により容易に得ることが
できる。また缶詰や、節類製造の際に副生ずる、魚介類
の頭部や、腹部を原料とするときは非常に経済的に製造
することが出来る。さらにまた、その中でもハラモを原
料とするときは天然の鰹節のフレーバーを非常に濃厚に
有する調味料が得られる。
In this way, the present invention does not use expensive bonito flakes as a raw material, but instead uses relatively easily available seafood as a raw material, it has the rich flavor of natural bonito flakes, contains a lot of amino acids, especially glutamic acid, and has a delicious taste. A rich and transparent seasoning can be easily obtained by relatively simple screening means such as filter cloth filtering without using screening aids such as diatomaceous earth. In addition, it can be produced very economically when the heads and abdomens of seafood, which are by-products during the production of canned goods and joints, are used as raw materials. Furthermore, among these, when Haramo is used as a raw material, a seasoning having a very rich flavor of natural bonito flakes can be obtained.

以下、実施例を示して本発明を更に具体的に説明する。Hereinafter, the present invention will be explained in more detail with reference to Examples.

実  施  例  1 生カツオから鰹節製造の際に副生じた腹内の部分「ハラ
モ」(脂質が多く節類製造には不適の部分)を常法によ
り煮熟処理し、次いで水切りした後薫煙処理室にて、4
日間焙乾処理し、水分的30%の処理物を得た。次いで
上記の焙乾処理物を粒経約5mmに割砕したもの500
gと水21を混和し、100℃、20分煮熟した後室温
まで放冷する。次いで、これにプロテアーゼとしてヤク
ルト社製アロアーセAP−10を2 、5g(2500
00単位)及び同社製バンチダーゼ即−2を10g(4
00000単位)を添加しさらにリノく一ゼとして8糖
産業社製リパーゼOF 0.05g(18000単位)
を添加し、45℃、6時間、攪拌しながらpH7,0に
て加水分解した。
Example 1 The abdominal part "haramo" (a part unsuitable for producing dried bonito flakes due to its high fat content), which is a by-product of producing dried bonito flakes from raw bonito, is boiled in a conventional manner, then drained and smoked. In the processing room, 4
It was roasted and dried for several days to obtain a treated product with a moisture content of 30%. Next, the above roasted and dried product was crushed into particles with a grain size of about 5 mm.
g and water 21 are mixed, boiled at 100°C for 20 minutes, and then allowed to cool to room temperature. Next, 2.5 g (2500 g
00 units) and 10 g (4
00,000 units) was added, and 0.05 g (18,000 units) of lipase OF manufactured by Octosu Sangyo Co., Ltd. was added as linokuichize.
was added and hydrolyzed at pH 7.0 at 45° C. for 6 hours with stirring.

次いで、腐敗防止のため食塩を終り濃度12%(w/W
)となるように添加し、さらに同条件で18時間加水分
解し、分解を終了した。
Next, to prevent spoilage, add salt to a final concentration of 12% (w/w).
) and further hydrolyzed under the same conditions for 18 hours to complete the decomposition.

この分解液をオートクレーブにて100°Cl2O分加
熱処理し、ナイロン製の濾布にて濾過し、本発明の調味
液を得た。
This decomposition liquid was heated in an autoclave for 100° C12O, and filtered through a nylon filter cloth to obtain a seasoning liquid of the present invention.

なお、比較のため上記本発明の調味料の製造法においで
「ハラモの焙乾処理物」を用いる代りに、「ハラモを焙
乾しないで水分を本発明の焙乾処理物と同程度に乾燥し
たもの」を用いる以外は全く同様にして、対照の調味液
を得た。
For comparison, in the method for producing the seasoning of the present invention, instead of using "roasted Haramo", "Haramo was dried to the same extent as the roasted Haramo without being roasted". A control seasoning liquid was obtained in exactly the same manner except that the same liquid was used.

また、上記本発明の調味液の製造法において、「リパー
ゼ」を用いない以外は全く同様にして、対照の調味液を
得た。
In addition, a control seasoning solution was obtained in exactly the same manner as in the method for producing a seasoning solution of the present invention except that "lipase" was not used.

上記で得られた3種類の調味液の、総窒素濃度、濁度、
及び風味について官能検査を実施した。
Total nitrogen concentration, turbidity, and
A sensory test was conducted regarding the taste and taste.

その結果を第1表に示す。The results are shown in Table 1.

第1表の結果から、比較例1に示すように焙乾処理をし
ない原料を用いるときは、生のカツオ由来の生臭味が製
品に移行し官能上良くない結果が得られ、また比較例2
に示すようにリパーゼを併用しないときは濁度の少ない
、即ち透明な調味料が得られない。
From the results in Table 1, when using raw materials that are not roasted and dried as shown in Comparative Example 1, the raw odor derived from raw bonito transfers to the product, resulting in poor sensory results. 2
As shown in Figure 2, when lipase is not used in combination, a seasoning with low turbidity, that is, a transparent seasoning cannot be obtained.

これに対し、本発明によれば原料として焙乾処理した魚
介類を用い、酵素としてプロテアーゼ及びリパーゼを併
用するものであるから、天然の鰹節の風味を有し、しか
も透明な調味料か得られることが判る。
In contrast, according to the present invention, roasted and dried seafood is used as the raw material, and protease and lipase are used together as the enzymes, so a transparent seasoning with the flavor of natural bonito flakes can be obtained. I understand that.

また、上記実施例1で得られjコ本発明の調味液のアミ
ノ酸組成を調べると第2表(こ示零−女口くであり、本
発明によればグルタミン酸のシト常(こ濃厚な、旨味の
非常に強い調味料か得られること力・半1jる。
In addition, when examining the amino acid composition of the seasoning liquid of the present invention obtained in Example 1, Table 2 shows that according to the present invention, the amino acid composition of the seasoning liquid of the present invention is very concentrated. A seasoning with a very strong flavor can be obtained by using half a cup.

Claims (2)

【特許請求の範囲】[Claims] (1)魚介類原料を酵素により加水分解して調味料を製
造するに当り、該原料として魚介類の焙乾処理物を用い
、また該酵素としてプロテアーゼ及びリパーゼを併用添
加することを特徴とする調味料の製造法。
(1) In producing a seasoning by hydrolyzing seafood raw materials with an enzyme, a roasted and dried seafood product is used as the raw material, and protease and lipase are simultaneously added as the enzymes. Method of manufacturing seasonings.
(2)プロテアーゼを、蛋白質g重量当り20〜350
0単位、リパーゼを、脂質g重量当り100〜7200
単位をそれぞれ添加する特許請求の範囲第1項記載の調
味料の製造法。
(2) Contain 20 to 350 proteases per gram of protein.
0 units, lipase from 100 to 7200 per g weight of lipid
The method for producing a seasoning according to claim 1, wherein each unit is added.
JP2295408A 1990-11-02 1990-11-02 Production of seasoning Pending JPH04169165A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2295408A JPH04169165A (en) 1990-11-02 1990-11-02 Production of seasoning

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2295408A JPH04169165A (en) 1990-11-02 1990-11-02 Production of seasoning

Publications (1)

Publication Number Publication Date
JPH04169165A true JPH04169165A (en) 1992-06-17

Family

ID=17820221

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2295408A Pending JPH04169165A (en) 1990-11-02 1990-11-02 Production of seasoning

Country Status (1)

Country Link
JP (1) JPH04169165A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003274898A (en) * 2002-03-20 2003-09-30 Nisshin Foods Kk Stock

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003274898A (en) * 2002-03-20 2003-09-30 Nisshin Foods Kk Stock

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