JPH04161298A - Apparatus for purifying water - Google Patents

Abstract

PURPOSE:To everlastingly prevent eutrophication due to the accumulation of nitrogen and phosphorus by purifying water by inherent bacteria in the storage tanks themselves of a series of floating type unit structures. CONSTITUTION:The position of a skirt is set by the anchors 5 connected to a platform 3. Periodically supplied inherent bacteria are supported in the floating type contact tanks 6 downwardly suspended from the platform 3. Further, inherent bacteria are periodically supplied to the contact tanks 6 by a bioreactor 7 and water accompanied by air is supplied to the contact tanks 6 by an air- water supply apparatus 8. The purified water subjected to biological treatment of nitrogen and phosphorus in the room 12 demarcated by a float 2 and the skirt 4, is sucked by the discharge apparatus 9 rising above the platform 3 and the float 2 from the area under the contact tanks 6 to face to the outside to be discharged to the outside of the room 12. As a result, the eutrophication due to the accumulation of nitrogen and phosphorus can be everlastingly prevented.

Description

[Detailed Description of the Invention] (Field of Industrial Application) The present invention utilizes the water purification effect of indigenous bacteria to significantly reduce nitrogen, phosphorus, etc. in artificial ponds and large storage tanks such as golf courses. Regarding water purification equipment.

(Conventional technology) Water quality standards for treated water discharged from sewage treatment facilities and large-scale wastewater purification equipment have traditionally regulated BOD, COD, SS, pH, etc. The reality is that there are no regulations on inorganic nutrient salts, nitrogen, phosphorus, etc., which are the causes of eutrophication. Most of the treatment and purification methods used in the above-mentioned facilities employ water purification methods that rely on physical and mechanical methods such as filtration, adsorption, and coagulation to dilute nitrogen and phosphorus in wastewater. .
(Problem to be solved by the invention) With the implementation of the total amount control in 1978, the target values for nitrogen and phosphorus were lowered as a countermeasure against eutrophication in rivers, lakes, and sea areas. There are calls for drastic reductions in nitrogen and phosphorus in purified treated water discharged from large-scale facilities before discharge.

In fact, the values for nitrogen and phosphorus contained in these effluents are far different from the values that are said to cause algal blooms (see Table 1).

＼Table 1 Therefore, there is a need for technology that can significantly reduce nitrogen, phosphorus, etc. in the storage tanks of these facilities that discharge large volumes of treated water.

The present invention was devised in order to solve these problems of the conventional technology, and an object of the present invention is to provide a novel water purification device consisting of a series of floating unit structures that perform purification using indigenous bacteria in the storage tank itself. shall be.

[Means for Solving the Problems] As a means for solving the above problems, a water purification device according to the present invention includes a float forming an annular weir on the water surface;
A platform stretched over the lower bottom surface of the float, a skirt whose upper end is fixed to the outer edge of the platform and whose lower end is lifted up to near the bottom sludge, an anchor connected to the platform to keep the skirt stationary, and the platform. a floating contact tank that is suspended downward from the platform and carries indigenous bacteria that is supplied periodically; a bioreactor that is installed on land and that periodically supplies indigenous bacteria to the contact tank; and a bioreactor that is installed on the platform and carries airborne bacteria. an air/water supply device for supplying water with water to the contact tank; and a discharge device that sucks purified water that has been subjected to biological treatment of phosphorus and phosphorus and discharges it to the outside of the room.

(Operation of the Invention) Endemic bacteria regularly supplied from the bioreactor are deposited in a contact tank in an underwater chamber.

Air-containing water passes through this contact tank, and the water circulates within this chamber and deposits nitrogen, phosphorus, etc., which are substances that cause eutrophication, into the contact tank. Feed the bacteria. Endemic bacteria use the nitrogen and phosphorus as nutrients to proliferate. In other words, nitrogen and phosphorus in the room are biologically treated by being converted into indigenous bacteria. Since this indigenous bacteria is harmless (see Experimental Example 1), the water in the room is first purified (see Experimental Example 2). Next, this purified water is discharged to the outside of the room along with endemic bacteria by a suction and drainage device. Because indigenous bacteria have a lower specific gravity than water, they are widely dispersed into the water outside the room, where they use the nitrogen and phosphorus as nutrients and multiply. As a result, water outside the room is subjected to biological treatment in the same manner as described above.

In this way, water in large storage tanks, artificial ponds, etc. is purified through biological treatment from part to part, and from there, the water is discharged into rivers, lakes, and sea areas, causing the phenomenon of eutrophication. This is not the case (see Figure 3).

(Embodiment) Hereinafter, a preferred embodiment of the present invention will be described in detail with reference to the drawings.

FIG. 1 is a side view showing an outline of a water purification device according to the present invention, and FIG. 2 is a plan view thereof.

In the figure, this water purification device 1 includes a float 2, a platform 3, a skirt 4, an anchor 5, a floating contact tank 6, a bioreactor 7, an air/water supply device 8, and a discharge device 9.
It is mainly composed of.

The float 2 constitutes an annular weir on the water surface 10,
A donut bag-like bag made of rubber or synthetic resin that holds air is often used, but there are no limitations in terms of material or structure as long as it has buoyancy. Moreover, when installing in a site close to a seawall, it can also be installed in a semi-annular shape so that both end surfaces are in contact with the seawall.

The platform 3 is stretched over the bottom surface of the float 2 and is equipped with an air/water supply device 8, a discharge device 9, and the like. The material of this platform 3 is mostly iron, but is not limited to iron.

The skirt 4 is made of rubber, and its upper end is fixed to the outer edge of the platform 3, and its lower end is lifted up so as to look closely at the bottom sludge 11. Therefore, float 2
A cylindrical chamber 12 is constituted by the skirt 4 and the skirt 4.

An anchor 5 is connected to the platform 3 to stationary the position of the room 12 according to the skirt 4. Preferably, it pierces the bottom sludge 11, but even if the bottom sludge 11 is not reached, the position of the chamber 12 remains stationary.

The floating contact tank 6 has a cylindrical mesh basket 13 filled with fibrous activated carbon (A
CF) 14, and a large number of them are suspended on the platform 3 with the axial direction perpendicular to the direction parallel to the axial direction of the chamber 12. A.C.
F14 is suitable as a culture medium for indigenous bacteria. Therefore, each floating contact tank 6 is periodically and automatically supplied with BSK bacteria, which is an endemic bacteria, from the bioreactor 7, and is then absorbed into the ACF 1 4.

Bioreactor 7 is installed on land and contains BS in an inactivated state.
It is a device that activates K bacteria and automatically grows, and many of them are
It is prepared as a bio-fluid and serves as a base for the growth and supply of BSK bacteria.

One bioreactor 7 often serves as a dedicated base for one water purification device 1, but it can also be used for multiple water purification devices 1.
It may also serve as a base for distributing and supplying.

The bioreactor 7 may be in direct communication with the floating contact tank 6, but in most cases it is in communication via an air/water supply device 8 to supply the BSK bacteria to the ACF 14 and cause them to settle therein. .

The BSK bacterium was discovered in the soil by the present inventors, belongs to the Bacillus subtilis family, is a Durum-positive bacterium that has antagonistic properties against harmful bacteria such as putrefaction bacteria, and is a reducing bacterium.
It is similar to standard Bacillus subtilis but has different properties. Under a microscope, they were observed as long chains when used alone or in pairs. This BSK bacterium has already been applied for as a biopharmaceutical, and was deposited as FEIKEN No. 9643 at that time.

The air/water supply device 8 includes a submersible pump 15 and a sealed tank 16 mounted below the platform 3, and the sealed tank 16.
It is composed of a water suction pump 17 and a bio-liquid suction hose 18 which are connected to the tank, a pipe 19 which is connected to the sealed tank 16, and which sprays water into the air from the center of the float 2.

The submersible pump 15 is configured to force-feed a mixture of water supplied into the closed tank 16 and the bio-liquid of BSK bacteria to the piping 19 to produce a water fountain. The sprayed bio-liquid falls into the chamber 12 with air and is sucked downward into the contact tank 6. At that time, the BSK bacteria are contained in the ACF of contact tank 6.
14, and the water passes through the contact tank 6 in contact with it at a constant speed. As the water comes into contact with the passage of room 12
Nitrogen and phosphorus inside are supplied to the BSK bacteria carrying them. BSK bacteria proliferate using nitrogen and phosphorus as nutritional sources,
Significantly reduces nitrogen and phosphorus in room 12. The water in the room 12 is purified by biologically treating the substances that cause clogging and eutrophication in the room 12 by ESK bacteria. Then, the BSK bacteria are further multiplied, and the multiplied BSK bacteria are released to the outside of the room 12 by the release device 9 along with water.

The discharge device 9 includes a submersible pump 20 mounted on the platform 3, a suction pipe 21 whose lower end faces below the contact tank 6, whose upper end is connected to the submersible pump 20, and whose lower end is connected to the submersible pump 20. and a discharge pipe 22 whose upper end extends over the float 2 and faces the outside.

Therefore, when the submersible pump 20 is operated, purified water that has been subjected to biological treatment is sucked from the suction pipe 21 and discharged from the water discharge pipe 22 to the outside of the room 12. Room 1 is contaminated with bacteria.
2. Spreads widely in the water outside. Therefore, nitrogen, phosphorus, etc., which are substances that cause eutrophication of external water, are biologically treated and purified by the BSK bacteria in the same manner as described above.

When such a series of actions is carried out and the number of BSK bacteria carried in the contact tank 6 decreases, this is detected by an appropriate method and the bioreactor 7 and the air/water supply device 8 are automatically activated to restart the operation. Purified water is generated through biological treatment of nitrogen and phosphorus by BSK bacteria, and the discharge device 9 discharges this water to the outside of the room 12, and as described above, the outside water is also purified.

In this way, by repeating a series of actions,
Gradually, the external water metal body undergoes biological treatment and is purified.

(Effects of the Invention) As described above, since the water purification device according to the present invention is a floating type and a unit type, the manufacturing device is simple.

Multiple large storage tanks will be installed to permanently deplete nitrogen.
Since it is possible to prevent eutrophication due to phosphorus accumulation, it can be used to combat blue-green algae in artificial ponds.

In addition, BSK bacteria, which is an endemic bacterium, can speed up the biodegradation of most agricultural chemicals (Example 2), so it is useful for environmental measures for pond water at golf courses, and it can also be used as agricultural water by reducing nitrogen content. It can also be used to prevent blue-green algae.

[Experimental Example 1] In order to prove that BSK bacteria, which is one of the endemic bacteria, is harmless, the following acute toxicity test was conducted.

Acute toxicity test 1 Requested name: B.P.I.B. Co., Ltd. 2 Specimen name: BAKU- (BSK Bacteria-created preparation Route of administration: Oral 4 Test animals: male and female ddy-N mice produced by Japan Medical Science and Animal Materials Research Institute Co., Ltd.) Females were introduced when they were about 4 weeks old, and after one week of preliminary breeding to confirm that there were no abnormalities in their health, they were used for testing when they were about 5 weeks old.Males weighed 22g at the start of the test. , female weighed 18-20 g.

5 Room temperature 22±2°C 6 Test period May 24th to June 7th, 1990 7 Test solution preparation method After adding distilled water for injection to the sample, use a homogenizer to make a 10% (W/Vl suspension) A solution was prepared and used as a test solution.

8 Method of administration of test solution, etc. l) Administration method Used mice were administered from 10:00 p.m. on the day before administration to 1:00 a.m. on the day of administration.
After fasting for 13 hours until 1:00 p.m., the test solution was forcibly administered orally once using a gastric tube.

Feed provision was resumed 3 hours after administration of the sample.

2) Calculation method of LD5° value Probit method 3) Dose of sample For both male and female mice, each test solution was added at a rate of 20ml per 1kg of body weight of the mouse.
By administering 2000 mg/k as a specimen
g was administered.

4) Number of animals per test group 5 males and 5 females each 9 Test results The results are shown in Table-1.

Table 1: Mortality and Mortality Rate 1O Poisoning Symptoms No abnormalities were observed in both males and females immediately after administration, and no deaths occurred.

In addition, all the service mice started eating immediately after restarting feeding.

11 Autopsy findings All animals were sacrificed at the end of the test and macroscopically autopsied, but no abnormalities were observed in the major organs of either males or females.

12 Discussion 0Acute toxicity testing methods such as the ECD guidelines (April 11, 1986) stipulate that the maximum dose of specimen to mice is 2000 mg/kg.

In this test, as a limit test, this highest dose (1 level) was forcibly administered orally to mice once, and observations were made 2 weeks after administration.

The specimen was suspended in distilled water for injection [10% (W/V)] and administered at 20 rnl/kg.

As a result, no toxic symptoms thought to be caused by the administration of the sample were observed in either males or females, and no deaths occurred. In addition, the body weight during the test period was as shown in Table 2,
Both males and females developed normally.

From the above results, the oral LDIIo value of the sample is 2000.
It was estimated that it was more than mg/kg.

Table 2 Changes in body weight (g) Test location 821 Yoshikura, Narita City, Nichiba Prefecture Test period name: Japan Scientific Feed Association Scientific Feed Research Center Test title: Harunobu Noro, Toshimasa Shimizu, Sanzenjin Hanazumi Yuji Kazehaya・Chisato Yonemochi/Hyaku Kei Size table - 1 Solid side weight (male, g) of a working mouse - 2 Solid side weight (female, g) of a working mouse [Experiment example 2] BSK, one of the endemic bacteria In order to prove that bacteria have the ability to decompose pesticides, we conducted the following basic tests.

Basic test on the decomposition of pesticides by BSK bacteria 1, client name: B.P.B. Co., Ltd. 2, sample "Sunde Cool" BSK bacteria 3, test sample (hereinafter referred to as rBSK bacteria). Cultivate in a medium containing pesticides and test whether the pesticides are decomposed. The target pesticides are diazinon, isobrothiolane/flutolanil mixture, and TPN.

4. Test overview l) Test-1 In order to check in advance the concentration of pesticides at which BSK bacteria can grow, BSK bacteria were cultured in a medium containing a specified concentration of pesticides using an automatic nephelometer. The growth curve was obtained using

2) Test-2 BSK bacteria were added to a medium containing a pesticide at a concentration determined based on the results of Test-1, cultured with shaking at 35°C, and the amount of pesticide in the medium was measured over time.

5. Test Results and Discussion The results of Test-1 are shown in Figures 4 to 6, and the results of Test-2 are shown in Table-1.

From the results of Test-1, diazinon and isobrothiolane/flutolanil mixture had no effect on the growth of BSK bacteria even in the medium supplemented with 10 ppm. TPN
In the case of , the growth rate of BSK bacteria tended to slow down as the concentration increased, but growth was observed at all concentrations.

Considering the results of Test-1, the concentration remaining in the environment, the detection limit, etc., the concentration used in Test-2 was set at 1 ppm.

From the results of Test-2, it was found that for diazinon, TPN, or flutolanil, adding BSK bacteria was better for BSKS.
It was confirmed that the concentration of these pesticides was significantly reduced compared to the sterile culture medium. Regarding isobrothiolane, no obvious difference in concentration was observed depending on whether BSK bacteria were added or not.

Therefore, it was presumed that BSK bacteria degraded diazinon, TPN, and flutolanil.

Table-I Changes over time in pesticide concentration in the medium containing BSK bacteria (unit: ppm) Zinone No addition 0.96 1.02 0.66 0,
72 0.58 Ranil No additives 0.460.46 0
．． 46 0.46 0.46 Thiolane No additive
0.62'0.62 0.58 0.64
0.64*Flutolanil and isobrothiolane were tested by adding them to the same medium.

6. Test method l) Test pesticide ■ Diazinon ■ TPN ■ Isobrothiolane/flutolanil mixture (mixed at a weight ratio of 5:4) 2) Preparation of liquid medium for test High-pressure steam sterilized ordinary bouillon medium ( Ken Kagaku),
A pesticide methanol (10%) solution with a concentration 10 times the test concentration was added to 1/9 volume of the culture medium, and 15 ml of the solution was dispensed into an L-shaped test tube.

3) Test operation■ Test-1 0.01% BSK bacteria was added to a test liquid medium containing 10.1 or 0.01 ppm of pesticides, and an automatic nephelometer (
(manufactured by Toyo Kagaku Sangyo Co., Ltd.) at 35°C,
Growth curves were obtained by continuously measuring the absorbance at 60 nm.

In addition, a broth medium inoculated with BSK bacteria-free seeds was cultured in the same manner, and it was confirmed that there was no change in absorbance.

■Test-2 0.01% BSK bacteria was added to a test liquid medium to which pesticides were added at about 1 ppm, and cultured at 35°C.
5. After 7 and 10 days, the concentration of the pesticide in the medium was measured by gas chromadography. In addition, BSK as a target
A test liquid medium containing S sterilization was also tested in the same manner.

[Brief explanation of drawings]

Figure 1 is a side view of the water purification device according to the present invention, Figure 2 is a cross-sectional view along the line ■-■ in Figure 1, and Figure 3 shows how the same water purification device reduces nitrogen and phosphorus in an artificial pond. Graphs showing the conditions. Figure 4 is the growth curve of BSK bacteria in diazinon-supplemented medium, Figure 5 is the growth curve of BSK bacteria in TPN-supplemented medium, and Figure 6 is the growth curve of BSK bacteria in medium supplemented with flutolanyl-isobrothiolane mixture. It is a growth curve. Code 1... Water purification device, 2... Float, 3...
・Platform, 4... Skirt, 5... Anchor, 6... Contact tank, 7... Bioreactor, 8-
Air/water supply device, 9... Discharge device, 10... Water surface, 1
1...Sludge, 12...Room Patent Applicant: B.P.I.B. Co., Ltd. Agent Patent Attorney: Miki Segawa Chuobu 1v! J Figure 2 (''/,) Nitrogen Phosphorus removal test

Claims (1)

[Claims] a float forming an annular weir on the water surface; a platform stretched over the bottom surface of the float; a skirt whose upper end is fixed to the outer edge of the platform and whose lower end is lifted up to near the bottom sludge; An anchor that keeps the skirt stationary, a floating contact tank that is suspended downward from the platform and carries indigenous bacteria that is periodically supplied, and a floating contact tank that is installed on land and that periodically supplies indigenous bacteria to the contact tank. a bioreactor to be supplied; an air/water supply device provided on the platform to supply water with air to the contact tank; A water purification device comprising: a discharge device that sucks purified water that has been subjected to biological treatment of nitrogen and phosphorus in a room divided by a float and a skirt and discharges it to the outside of the room.