JPH04131811A - Microscopic device, and slide and condenser for microscopy - Google Patents

Abstract

PURPOSE:To increase and improve the quantity of light projected on a sample by freely fitting the peak part of an optical condenser part which faces an objective lens system, in a through hole formed in a stage, and providing a light converging means to the optical condenser part freely detachably and successively. CONSTITUTION:The luminous flux 5 from a light source 4 is deflected by a reflecting mirror 6, an optical condenser means 1B which has a condenser lens system so as to project light from below a preparation is freely fitted in the through hole 2A of the stage 2 and also arranged opposite the objective 1A. A condenser 40 equipped with a condenser lens 41 is fitted onto the peak part of the optical condenser means 1B and the condenser lens 41 is fitted in the recessed part 31Y of the slide 31 sufficiently to converge the luminous flux 5 sufficiently and irradiate the sample S. Consequently, the luminous flux 5 can be projected on the sample S at a short distance, an extremely bright and sharp image can be viewed, and the resolution is improved.

Description

Detailed Description of the Invention [Industrial Application Field 1] The present invention provides a microscopic examination device that precisely examines a sample captured on a filter using a microscope with the microscopic pores of the filter erased. and slides and concentrators for microscopic examination.

[Conventional technology 1 Conventionally, slides used for slides were made of glass (
Those made of inorganic materials and those made of organic heavy substances are widely known. Among these slides, glass slides do not undergo elastic deformation and are excellent in weather resistance and chemical resistance, but they require considerable care when handling due to the risk of breakage or breakage.

Recently, plastic slides have been proposed as an alternative to glass slides. This plastic slide is made of an organic polymer or the like, can be slightly elastically deformed, has little risk of breakage, is lightweight, and is inexpensive, so it is increasingly used.

Also, patent application No. 61-16244 related to the application earlier than this application.
As seen in the invention of No. 3, ceramics etc. are mixed into the organic polymer in order to diffuse the transmitted light.
The pore diameter of the attached filter is approximately 0015μm-14.0
There are also devices that are ingeniously designed so that microscopic pores on the order of μm are optically erased and cannot be visually recognized during microscopic examination.

That is, the sample is captured on a filter made of a thin film of polycarbonate or polyester with uniform filtration holes as described above, subjected to a staining process, and attached to a slide together with the filter to create a preparation. By applying a process to diffuse the transmitted light on the slide, only the sample can be accurately and efficiently microscopically examined. The addition of ceramics increases the strength, and the weather resistance and chemical resistance are far superior to those made only of organic polymers. It has been received with positive reviews.

[Problems to be Solved by the Invention] By the way, during microscopic examination, it is possible to improve the resolution by providing a sufficient amount of light that passes through the slide and is projected onto the sample.
Needless to say, this is an important requirement for spermatozoa examination, and for this reason, in addition to increasing the amount of light projected onto the slide, efforts have been made to condense the light to obtain bright episcopic light. This is where I am.

On the other hand, in conventional devices, the distance between the slide and the objective lens of the microscope is approximately 4 mm to 5 mrn, and the distance between the bottom surface of the slide and the condensing lens is 2 mm. This is considered to be a bottleneck technology when trying to increase and improve the amount of light projected onto the sample on the slide, and there are strong expectations for a solution to this problem.

[Means for Solving Problems 1 Effect] The present invention provides a solution to the above-mentioned problems, and its structural features are as set forth in the claims. This invention relates to a microscopic examination device that can be equipped with an organic polymer slide that is partially thinned by a recess and a light focusing means that is inserted into the recess. The bright beam of light is powerfully projected very close to the sample on the slide, even if the slide is made of an organic polymer mixed with a light diffusing material such as ceramics.
Even though the amount of light projected onto the sample is reduced by the amount of light being diffused, it is possible to provide a sufficient amount of light to clearly see the sample, and it is now possible to obtain something that satisfies the user's needs. be.

[Example 1] Next, the content of the present invention will be explained by referring to an example. As shown in FIG. 1, an objective lens IA is placed above the preparation 3 placed on the stage 2 of the microscopic examination device 1, while
A light beam 5 from a light source 4 is deflected by a reflecting mirror 6, and an optical condenser means IB equipped with a condensing lens system is connected to the through hole of the stage 2 so as to project it from below the slide 3.
It can be freely inserted into the lens A and is placed opposite the objective lens IA. The slide 31 constituting the pre-balanced sheet 3 is formed into a thin plate of ceramic or an organic polymer mixed with a light diffusing material 31A such as silicon particles, as shown in FIGS. 1 and 2. As shown in FIGS. 4 and 6, the top surface 31B is partially made into a finely roughened surface, and a display section 31C is provided that can be freely written on with a writing instrument, a stamp, etc., while a support frame is provided. After capturing the sample S on the filter membrane 33 having micropores 33A supported on the filter membrane 32 and subjecting it to appropriate staining treatment, the sample S is mounted on a cover glass 34 using a mounting medium to form a bleeppart 3. It is a hanging structure, and a recessed portion 31Y is formed in the bottom surface 31X on the other side.

Furthermore, a condenser 40 equipped with a condenser lens 41 is fitted onto the top of the optical condenser means IB which projects the light beam 5 onto the concave portion 31Y, as shown in FIGS. It is something.

When diagnosing a sample S using the microscopic examination apparatus 1 configured as described above, the lifting adjustment drive means C is operated and the horizontal movement means H is operated to move the optical condenser means IB to the objective lens I.
By arranging the light beam 5 in the desired relationship with respect to A and fitting the condensing lens 41 of the condenser 40 sufficiently into the recessed part 31Y of the slide 31, the light beam 5 can be sufficiently condensed and irradiated onto the sample S. In addition, an elevating drive means (not shown)
By bringing the objective lens IA close to the cover glass 34 of the preparation 3, a microscopic examination is performed.

In this case, the sample S that is visually recognized through the eye lens (not shown) is caused by the condensing lens 41 of the condenser 4o being deeply inserted into the recessed part 31Y of the slide 31, and as a result, the sample S is viewed from a close distance. Since the light beam 5 is projected, it is extremely bright and can be seen clearly, and the resolution is greatly improved.Moreover, the light beam 5 projected onto the slide 31 is inside the slide 31 made of organic polymer. Since the light is diffused by the mixed diffusion material 31A such as ceramic fine particles, the micropores 33A of the filter membrane 33 are erased and are not visible, allowing accurate microscopic examination without misdiagnosis. It can be said to be a thing.

Note that the shape, dimensions, number, arrangement, etc. of the recessed portion 31Y of the slide 31 are not limited to a single quadrilateral shape, and may of course be arbitrarily selected depending on the usage. It goes without saying that there is. In addition, the display portion 31C has a finely roughened surface,
It is designed to prevent the necessary information from being abraded and erased by hands and fingers when writing the necessary information in the core part 31C with a pencil, felt-tip pen, etc., but it is sufficient as long as the displayed contents do not disappear. For example, it can be said that the function of the display section 31C is not impaired even if cellophane tape is pasted on the display area after display processing, or lacquer is sprayed after writing.

[Effects of the Invention] Since the present invention has the contents described in detail above, the sample on the organic heavy content slide is illuminated with a light beam from a position that is deep (inserted into the recessed part and sufficiently approached). The projection optical system means that enough light is projected for microscopic examination, but the microscopic pores in the filter membrane are erased and cannot be visually recognized.
The quality of the image has been improved, and the box can be easily used for microscopic examination, and the contents of the microscopic examination can be written on the display section, making it possible to effectively use it as a preparation for storage.

[Brief explanation of drawings]

FIG. 1 is a partially longitudinal side view of a microscopic examination device according to the present invention;
Figure 2 is a perspective view of the condenser, Section 3 is an enlarged vertical sectional view of part 11 in Figure 1, Figure 4 is a front view of the slide, Figure 5 is a rear view thereof, and Figure 6 4 shows a sectional view taken along line 4--2. 1...Microscopic examination device 3...Preparation 31...Slide 31C...Display section 31Y...Recessed part 40...Concentrator

Claims (4)

[Claims] (1) The top of the optical condenser placed opposite the objective lens system in the microscope can be freely inserted into a through hole made in the stage, and the condensing means equipped with a condensing lens can be attached to and removed from the optical condenser. A microscopic examination device characterized by being freely connected. (2) A slide for microscopic examination consisting of a slide made of an organic polymer mixed with a light diffusing material such as ceramics or silicon particles, and a recessed portion formed on at least one surface of the slide. (3) The slide for microscopic examination according to claim 2, further comprising a display section formed of a finely rough surface section in an area other than the recessed section of the slide. (4) comprising a condensing lens such as a concave lens or a convex lens, the top of which can be freely fitted into the recess of the slide;
A condenser that can be freely installed on the optical system of the optical condenser section of a microscope.