JPH0412956B2 - - Google Patents

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Publication number
JPH0412956B2
JPH0412956B2 JP63262575A JP26257588A JPH0412956B2 JP H0412956 B2 JPH0412956 B2 JP H0412956B2 JP 63262575 A JP63262575 A JP 63262575A JP 26257588 A JP26257588 A JP 26257588A JP H0412956 B2 JPH0412956 B2 JP H0412956B2
Authority
JP
Japan
Prior art keywords
abscisic acid
medium
culture
natural abscisic
corn
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63262575A
Other languages
Japanese (ja)
Other versions
JPH02109988A (en
Inventor
Masao Ito
Eisuke Motokawa
Shingo Marumo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shin Nihon Kagaku Kogyo KK
Original Assignee
Shin Nihon Kagaku Kogyo KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shin Nihon Kagaku Kogyo KK filed Critical Shin Nihon Kagaku Kogyo KK
Priority to JP26257588A priority Critical patent/JPH02109988A/en
Publication of JPH02109988A publication Critical patent/JPH02109988A/en
Publication of JPH0412956B2 publication Critical patent/JPH0412956B2/ja
Granted legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

【発明の詳細な説明】[Detailed description of the invention]

(産業上の利用分野) 本発明は微生物の代謝機能を利用した天然型ア
ブシジン酸の製造法に関し、生産量を著しく増加
し、かつ夾雑物が少なく精製を容易にできる方法
に係るものである。 (従来の技術) 天然型アブシジン酸は1963年カリフオルニア大
学のアデイコツトらにより棉の幼果の落果促進物
質として初めて単離され、それ以後、種々の植物
細胞に対する重要な生理作用が見い出され、今日
では巾広い生理活性を有する植物ホルモンとして
認められている。 天然型アブシジン酸の植物生理作用の特徴は他
の植物ホルモンであるオーキシン、ジベレリン、
サイトカイニン等の促進作用と拮抗する抑制作用
をもつことにあるが、そのうちでも注目すべき作
用の一つは他の植物ホルモンと異なつて植物気孔
の閉鎖作用を持つことである。将来天然型アブシ
ジン酸はこのような生理作用を利用して、干魃、
冷害等の気候変化に対応できる物質として農業生
産への応用が期待され、さらにビール醸造におけ
る品質向上とか、価格低廉化(特開昭58−101677
号公報参照)等の醸造への利用、人工種子製造に
おける不定胚の発芽促進利用開発等が期待されて
いる。 従来アブシジン酸の製造法としては有機合成的
方法を利用していたものが殆どで、これらの製造
法では光学活性を有する天然型アブシジン酸を得
ることは極めて困難であり、これを得られる特殊
な製造法が考えられ無いでは無いが製造費が高価
となり、上記の農業、醸造とか食品製造に利用す
るにはコスト高になつて実質的に使用不可能であ
つた。 このため、有機合成法に代るものとして微生物
の代謝機能を利用した発酵生産が有力手段として
検討されるに至り、本発明者等の一人によりボト
リチス菌による製造法(特公昭61−35838)、及び
セルコスポラ・ロシコラ[E xperientia,33,
1556(1977)]とその改良法(特公昭59−45359、
特公昭60−6629)が発明されて公知になつてい
る。さらにボトリチス菌の培養において柑橘類果
皮の添加によりアブシジン酸の生産量が増加する
ことも確認されている。 しかし上記の微生物を利用した製造法の最大の
欠点は発酵生産による天然型アブシジン酸の蓄積
量が極めて低く、かつ発酵速度も遅く、天然型ア
ブシジン酸の工業的規模の生産手段としては極め
て効率の悪い方法である。また柑橘類果皮添加の
場合は夾雑物が多くて精製費が高価になる問題点
がある。 (発明が解決しようとする問題点・本発明の目
的) 本発明者等は、天然型アブシジン酸を効率良く
生産する方法に関して鋭意検討を加え、前記した
問題点を解決することを目的としたものである。 (問題点を解決するための手段) 本発明は、実験の繰り返しにより、不完全菌の
一種であるボトリチス(Botrytis)属に属し、天
然型アブシジン酸生産能を有する菌株を培養する
に当たり、培地中に窒素源および菌の生育あるい
は天然型アブシジン酸の生産に必要な微量成分の
補給源としてコーン、コーン胚芽、ホミニーフイ
ード(とうもろこしヌカ)を単独にまたは2種以
上混合して添加することにより天然型アブシジン
酸の生産量が増加し、また添加物によつては生産
量が多少劣つても夾雑物が少なく、精製費用を大
巾に低下させ得ること、および充分工業化し得る
ことを見い出して本発明を完成した。 すなわち本発明は、ボトリチス属に属し、天然
型アブシジン酸生産能を有する菌株を前記した剤
を添加した培地に接種し、適切な条件で培養し、
該培養物中より天然型アブシジン酸を採取するこ
とを特徴とする天然型アブシジン酸の製造方法で
ある。 以下本発明の詳細を述べる。 本発明に使用する菌株はボトリチス属に属する
天然型アブシジン酸生産菌であり、これには通常
の変異菌や変異処理によつて生じた菌株をも含む
ものとする。 ボトリチス菌に属する天然型アブシジン酸生産
菌種の具体例として、ボトリチス・シネレア
(Botrytis cinerea)微工研菌寄第6156号(特公
昭61−35838)があげられる。 本発明は、後に記載するように前記した培地に
前記した剤を添加し、適切な方法によつて滅菌
し、前記のボトリチス属に属する天然型アブシジ
ン酸生産菌株の胞子又は菌糸体部分の水懸濁液を
培地1g当たり1×102個細胞以上接種し培養する。
培養は静置培養、振とう培養、若しくは通気培養
で行う。培養条件は温度10〜35℃、好ましくは20
〜30℃、培地のPHは3〜12、好ましくは4〜8、
期間は1〜15日間、好ましくは3〜9日間であ
る。期間は培養状態により決める。 培養終了後、有機化合物の精製法を応用するこ
とも可能であり、一般的な精製法により単離精製
することが可能である。 (原料菌株の液体培養の検討) 現在のところ天然型アブシジン酸の微生物生産
はボトリチス菌とセルコスポラ菌のみで報告され
ている。このうちセルコスポラ菌は液体培養で実
施されているがその培養液からのアブシジン酸の
収量は低くて採算に合わない。 ボトリチス菌はこれまで天然型アブシジン酸を
固体培養でよく生産するが、液体培養では殆んど
生産しないとされていた。 本発明に用いる培地は固体培地、液体培地のい
ずれでも良い。 しかし液体培養が培養管理上極く有利であるか
ら、本発明の検討に当たりボトリチス菌が液体培
養で天然型アブシジン酸を生産するかを検討し
た。本検討の終始は次の通りである。 最近に本発明者の一人が他の者との共同研究に
より、フスマ熱水抽出物とみかん果皮熱水抽出物
を添加した培地により液体培養したところ高率の
収量を得た(未公知)。本発明者等はこれとは別
個に液体培養について検討し、前記のみかん果皮
の他に本発明の培地添加物であるコーン、ホミニ
ーフイード、コーン胚芽を添加し、従来の無添加
培地による培養とを比較検討した。 その比較検討に使用した培地と培養手順は、バ
レイシヨ抽出液の4倍希釈液(この100ml中には
グルコース0.5g、バレイシヨ5g相当分の抽出物を
含む)に、コーンスチープリカー0.1g、炭酸カル
シウム2gを加えた培地80mlを300ml容三角フラス
コに分注し、120℃20分間滅菌したものを対照培
地(基本培地)とした。この他に、みかん果皮添
加培地を第2対照培地とした。先の基本培地(対
照培地)に、コーン、コーン胚芽、ホミニーフイ
ードの一種又は二種類以上添加した本発明の培地
(添加物の添加量は表−1に示す)を調製し、こ
れらの二種の対照培地と本発明の培地とにボトリ
チス・シネレア菌(FERM、P−6156)の胞子
又は菌糸体の濃厚懸濁液を培地1ml当たり1×
102個細胞以上を接種し、25℃で7日間回転振と
う培養(200rpm)を行ない、得られた培養物を
前記と同様に処理した結果を表−1に示す。
(Industrial Application Field) The present invention relates to a method for producing natural abscisic acid using the metabolic functions of microorganisms, and relates to a method that can significantly increase the production amount, contain few impurities, and facilitate purification. (Prior art) Natural abscisic acid was first isolated in 1963 by Adikotsuto et al. of the University of California as a substance that promotes fruit drop in young cotton fruits.Since then, it has been discovered that it has important physiological effects on various plant cells, and today it is known as abscisic acid. It is recognized as a plant hormone with a wide range of physiological activities. The plant physiological action of natural abscisic acid is characterized by the presence of other plant hormones such as auxin, gibberellin,
It has an inhibitory effect that antagonizes the promoting effect of cytokinins, etc., but one of its most noteworthy effects is that, unlike other plant hormones, it has a closing effect on plant stomata. In the future, natural abscisic acid will utilize these physiological effects to help with drought,
It is expected to be applied to agricultural production as a substance that can cope with climate changes such as cold damage, and it can also be used to improve the quality of beer brewing and reduce the price (Japanese Patent Application Laid-Open No. 58-101677
It is expected to be used in brewing (see the above publication), and to promote the germination of somatic embryos in the production of artificial seeds. Conventionally, most methods for producing abscisic acid have used organic synthesis methods, and it is extremely difficult to obtain optically active natural abscisic acid using these production methods. Although the production method is not inconceivable, the production cost is high, and it is practically impossible to use it for the above-mentioned agriculture, brewing, or food production. For this reason, fermentation production using the metabolic functions of microorganisms has been considered as a promising alternative to organic synthesis methods, and one of the present inventors has developed a production method using Botrytis bacteria (Japanese Patent Publication No. 61-35838). and Cercospora rosicola [E xperientia, 33,
1556 (1977)] and its improved method (Special Publication No. 59-45359,
Japanese Patent Publication No. 60-6629) was invented and is now well known. Furthermore, it has been confirmed that the production of abscisic acid increases when citrus peel is added to the culture of Botrytis bacteria. However, the biggest drawback of the above production method using microorganisms is that the amount of natural abscisic acid accumulated through fermentation production is extremely low, and the fermentation rate is also slow, making it extremely inefficient as a means of producing natural abscisic acid on an industrial scale. That's a bad method. In addition, when citrus peel is added, there is a problem that there are many impurities and the refining costs are high. (Problems to be Solved by the Invention/Object of the Invention) The present inventors have conducted intensive studies on a method for efficiently producing natural abscisic acid, and have aimed to solve the above-mentioned problems. It is. (Means for Solving the Problems) The present invention, through repeated experiments, has revealed that, in culturing a strain belonging to the genus Botrytis, which is a type of Deuteromycetes, and having the ability to produce natural abscisic acid, Natural abscisin can be produced by adding corn, corn germ, and hominy feed (corn bran) singly or in a mixture of two or more as a nitrogen source and a supplementary source of trace components necessary for bacterial growth or production of natural abscisic acid. The present invention was developed based on the discovery that the production amount of acid can be increased, and even if the production amount is slightly inferior depending on the additive, there are fewer impurities, and that the refining cost can be greatly reduced, and that it can be fully industrialized. completed. That is, the present invention involves inoculating a strain belonging to the genus Botrytis and having the ability to produce natural abscisic acid into a medium supplemented with the above-mentioned agent, culturing it under appropriate conditions,
This is a method for producing natural abscisic acid, which is characterized by collecting natural abscisic acid from the culture. The details of the present invention will be described below. The strain used in the present invention is a naturally occurring abscisic acid-producing bacterium belonging to the genus Botrytis, and includes normal mutant bacteria and strains produced by mutation treatment. A specific example of a naturally occurring abscisic acid-producing bacterial species belonging to the Botrytis bacterium is Botrytis cinerea, Microtechnical Research Institute No. 6156 (Japanese Patent Publication No. 61-35838). The present invention involves adding the above-mentioned agent to the above-mentioned medium as described later, sterilizing it by an appropriate method, and suspending the spores or mycelium of the above-mentioned naturally occurring abscisic acid-producing strain belonging to the genus Botrytis in water. Inoculate the suspension at 1×10 2 or more cells per gram of medium and culture.
Culture is performed by static culture, shaking culture, or aeration culture. Culture conditions are temperature 10-35℃, preferably 20℃
~30°C, pH of the medium is 3-12, preferably 4-8,
The period is 1 to 15 days, preferably 3 to 9 days. The period is determined depending on the culture condition. After completion of the culture, it is also possible to apply a purification method for organic compounds, and it is possible to isolate and purify by a general purification method. (Study of liquid culture of raw material strains) Currently, microbial production of natural abscisic acid has been reported only in Botrytis and Cercospora bacteria. Among these, Cercospora bacteria are cultured in liquid, but the yield of abscisic acid from the culture solution is low and unprofitable. Until now, Botrytis bacteria was thought to produce natural abscisic acid well in solid culture, but hardly to produce it in liquid culture. The medium used in the present invention may be either a solid medium or a liquid medium. However, since liquid culture is extremely advantageous in terms of culture management, in studying the present invention, we investigated whether Botrytis bacteria can produce natural abscisic acid in liquid culture. The details of this study are as follows. Recently, one of the inventors of the present invention, in collaboration with others, obtained a high yield when liquid culturing was carried out using a medium to which a hot water extract of bran and a hot water extract of mandarin peel were added (unknown). Separately, the present inventors investigated liquid culture, and added corn, hominy feed, and corn germ, which are the medium additives of the present invention, in addition to the above-mentioned tangerine peel, thereby making it different from culture using conventional additive-free medium. A comparative study was conducted. The medium and culture procedure used for the comparative study were a 4-fold dilution of potato extract (100 ml contains extract equivalent to 0.5 g of glucose and 5 g of potato), 0.1 g of corn steep liquor, and calcium carbonate. 80 ml of the medium to which 2 g was added was dispensed into a 300 ml Erlenmeyer flask and sterilized at 120°C for 20 minutes, which was used as a control medium (basic medium). In addition, a medium supplemented with mandarin peel was used as a second control medium. A medium of the present invention was prepared by adding one or more types of corn, corn germ, and hominy feed to the above basic medium (control medium) (the amounts of additives are shown in Table 1). A concentrated suspension of spores or mycelium of Botrytis cinerea (FERM, P-6156) was added to the control medium and the medium of the present invention at 1× per ml of medium.
At least 10 2 cells were inoculated, cultured with rotational shaking (200 rpm) at 25°C for 7 days, and the resulting culture was treated in the same manner as described above. The results are shown in Table 1.

【表】 上表で分かるように第1対照の無添加培地によ
る天然型アブシジン酸生産量と第2対照を含むコ
ーン、ホミニーフイード、コーン胚芽を添加した
培地による生産量とは格段の違いがあつたが、培
養液の着色情況はみかん果皮を用いた第2対照の
場合は、顕著な着色と濁りが認められ、コーン、
ホミニーフイード、コーン胚芽を添加物とした培
養液は僅かしか着色せず濁りも少ないから精製上
で極めて有利で実用的と考えられた。 (本発明の固体培養による天然型アブシジン酸の
定量) フスマ10g、炭酸カルシウム1g、水10mlを500
ml容三角フラスコ中で撹拌混合し、120℃20分間
加熱滅菌したものを対照培地とし、フスマ7g、
炭酸カルシウム1g、コーンフラワー3g、水10ml
を500ml容三角フラスコ中で撹拌混合し、120℃20
分間加熱滅菌したものを対照培地とする。次
に、フスマ、炭酸カルシウム、水からなる基本培
地にコーン、コーン胚芽、ホミニーフイード(と
うもろこしヌカ)の一種又は二種以上を添加した
培地を本発明の培地〜とし(培地組成は表−
2に示す)前記と同様に加熱滅菌して調製する。
これ等の対照培地,と本発明の培地〜と
にボトリチス、シネレア菌(FERM、P−6156)
の胞子又は菌糸体の濃厚懸濁液を培地1g当たり
1×102個細胞以上を接種し、25℃で7日間静置
培養した。 培養終了後、各試験区の培養物を夫々20倍量の
水、エタノールまたはメタノール中に約2時間浸
漬抽出し、次いで、水、エタノールまたはメタノ
ール抽出液を減圧濃縮した濃縮液をPH9.0に調節
し、酢酸エチルで抽出洗浄した。抽出から残つた
水溶液をPH3.0に調節した後、酢酸エチルで抽出
し、得られた酢酸エチル液を無水硫酸ナトリウム
で乾燥後濃縮し、酢酸エチル可溶性酸性区分を
得、その一定量をシリカゲル薄層にスポツトし、
酢酸エチル1:n−ヘキサン1の溶媒で展開して
天然型アブシジン酸の蓄積量を表−2の通り定量
した。
[Table] As can be seen from the table above, there was a marked difference between the production amount of natural abscisic acid in the first control medium without additives and the production amount in the second control medium containing corn, hominy feed, and corn germ. However, in the case of the second control using mandarin peel, the coloring of the culture solution was markedly colored and turbid, and corn,
A culture solution containing hominy feed or corn germ as an additive is only slightly colored and has little turbidity, so it is thought to be extremely advantageous and practical for purification. (Quantification of natural abscisic acid by solid culture of the present invention) 10 g of bran, 1 g of calcium carbonate, and 10 ml of water
A control medium was prepared by stirring and mixing in a ml Erlenmeyer flask and sterilizing the mixture by heating at 120°C for 20 minutes.
1g calcium carbonate, 3g cornflour, 10ml water
Stir and mix in a 500ml Erlenmeyer flask and heat at 120℃20
The control medium is one that has been heat sterilized for a minute. Next, a medium in which one or more types of corn, corn germ, and hominy feed (corn bran) were added to a basic medium consisting of bran, calcium carbonate, and water was used as the medium of the present invention (medium composition is shown in Table 1).
2) Prepare by heat sterilization in the same manner as above.
These control media, and the media of the present invention include Botrytis and Cinerea (FERM, P-6156).
A concentrated suspension of spores or mycelium was inoculated at 1×10 2 or more cells per gram of medium, and cultured stationary at 25° C. for 7 days. After culturing, the culture of each test plot was extracted by immersion in 20 times the volume of water, ethanol, or methanol for about 2 hours, and then the water, ethanol, or methanol extract was concentrated under reduced pressure to a pH of 9.0. The mixture was adjusted and extracted and washed with ethyl acetate. After adjusting the aqueous solution remaining from the extraction to PH3.0, it was extracted with ethyl acetate, and the obtained ethyl acetate solution was dried over anhydrous sodium sulfate and concentrated to obtain an ethyl acetate-soluble acidic fraction, and a certain amount of it was poured into a silica gel thin layer. Spotted on the layer,
It was developed with a solvent of ethyl acetate 1:n-hexane 1, and the accumulated amount of natural abscisic acid was quantified as shown in Table 2.

【表】【table】

【表】 上記の表−2によつて明らかであるように、本
発明の剤を加えないで炭酸カルシウムとフスマの
みを添加して調製した培地からなる対照区及び
炭素源であるコーンフラワーを添加した対照区
、本発明の実施例区〜の天然型アブシジン
酸の生産蓄積量は表−2に記載の通りで、対照区
の蓄積量8mgに対して実施例区〜は約17倍
〜14倍、対照区の蓄積量40mgに対して実施例区
〜は約3.5倍〜2.8倍であつて、窒素源および
菌の生育あるいは天然型アブシジン酸の生産に必
要な微量成分の補給源としてコーン、コーン胚
芽、ホミニーフイード(とうもろこしヌカ)の一
種又は二種以上添加した培地が天然型アブシジン
酸の製造に極めて適合することを前記の定量によ
り確認し得た。前記は標品(米国シグマケミカル
社製)と比較して確認したものである。 (実施例) フスマ7g、みかん果皮(乾燥)3g、炭酸カル
シウム1g、水10mlを500ml容三角フラスコに分注
し120℃20分加圧滅菌した対照培地と表−3に示
す実施例区〜の通りの組成にした本発明の培
地とにより前記と同じ手順に従つて培養、抽出、
精製を行つて天然型アブシジン酸の生成を定量し
た。その結果を表−3に示した。
[Table] As is clear from Table 2 above, a control group consisting of a medium prepared by adding only calcium carbonate and bran without the addition of the agent of the present invention, and a control group consisting of a medium prepared by adding only calcium carbonate and bran, and a control group consisting of a medium prepared by adding only calcium carbonate and bran, and a control group containing a medium prepared by adding corn flour as a carbon source The accumulated amount of natural abscisic acid produced in the control plot and the example plot of the present invention is as shown in Table 2, and the accumulation amount in the example plot is about 17 to 14 times that of 8 mg in the control plot. The accumulation amount in the example plots was approximately 3.5 to 2.8 times that of 40 mg in the control plot, and corn and corn were used as a nitrogen source and a supplementary source of trace components necessary for bacterial growth and production of natural abscisic acid. It was confirmed by the above quantitative determination that the medium containing one or more of germ and hominy feed (corn bran) is extremely suitable for producing natural abscisic acid. The above was confirmed by comparing with a standard product (manufactured by Sigma Chemical Co., USA). (Example) 7 g of bran, 3 g of tangerine peel (dried), 1 g of calcium carbonate, and 10 ml of water were dispensed into a 500 ml Erlenmeyer flask and sterilized under pressure at 120°C for 20 minutes.The control medium and the example groups shown in Table 3 were used. Cultivation, extraction and
Purification was performed and the production of natural abscisic acid was quantified. The results are shown in Table-3.

【表】 実施例区〜での培養物からの抽出液は、対
照区の抽出液と比較して色度(430nmに於ける吸
光度)において約1/9、乾物量において約1/4であ
つて、アブシジン酸以外の夾雑物の量が格段に低
く、精製費用の低廉さが収量の若干の低さを補つ
て余り有ることを確認し得た。 (作用及び効果) 本発明は前記によつて明らかにしたように、天
然型アブシジン酸生産能を持つ菌体を培養する培
地に窒素源および菌の生育あるいは天然型アブシ
ジン酸の生産に必要な微量成分の補給源としての
コーン、コーン胚芽、ホミニーフイード(とうも
ろこしヌカ)を単独にまたは2種以上混合して添
加することにより天然型アブシジン酸を、他の培
地により生産するに比して著増することが出来
た。柑橘類果皮を培地に添加するときは生産量の
み増加することが出来るが、この時は夾雑物が多
いため抽出液を濁らせかつ精製を困難にする。し
かるに本発明によつて生産した培養物には夾雑物
がほとんどなく澄んだ抽出液が得られ、若干の生
産量不足を充分に補うことが出来る効果を持つ。
[Table] The extracts from the cultures in the example plots ~ have approximately 1/9 of the chromaticity (absorbance at 430 nm) and approximately 1/4 of the dry matter content compared to the extracts of the control plots. It was confirmed that the amount of impurities other than abscisic acid was extremely low, and that the low purification cost more than compensated for the slightly low yield. (Functions and Effects) As clarified above, the present invention provides a medium for culturing bacterial cells capable of producing natural abscisic acid, including a nitrogen source and a trace amount necessary for bacterial growth or production of natural abscisic acid. By adding corn, corn germ, and hominy feed (corn bran) as supplementary sources of ingredients, either singly or in combination of two or more, natural abscisic acid can be significantly increased compared to production using other media. was completed. When citrus peel is added to the culture medium, only the production can be increased, but in this case there are many impurities that make the extract cloudy and difficult to purify. However, the culture produced according to the present invention has almost no impurities and a clear extract can be obtained, which has the effect of being able to sufficiently compensate for the slight production shortage.

Claims (1)

【特許請求の範囲】[Claims] 1 ボトリチス属に属し、天然型アブシジン酸生
産能を有する菌株を培地に培養し、培養物中に天
然型アブシジン酸を蓄積せしめ、該培養物から天
然型アブシジン酸を採取して天然型アブシジン酸
を製造するに際し、培地中に窒素源および菌の生
育あるいは天然型アブシジン酸の生産に必要な微
量成分の補給源としてコーン、コーン胚芽、ホミ
ニーフイード(とうもろこしヌカ)を単独にまた
は2種以上混合して添加することを特徴とする天
然型アブシジン酸の製造方法。
1. A strain belonging to the genus Botrytis and having the ability to produce natural abscisic acid is cultured in a medium, natural abscisic acid is accumulated in the culture, and natural abscisic acid is collected from the culture to produce natural abscisic acid. During production, corn, corn germ, and hominy feed (corn bran) are added to the culture medium, either singly or in a mixture of two or more, as a nitrogen source and a supplementary source of trace components necessary for bacterial growth or production of natural abscisic acid. A method for producing natural abscisic acid, characterized by:
JP26257588A 1988-10-18 1988-10-18 Production of natural type abscisic acid Granted JPH02109988A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP26257588A JPH02109988A (en) 1988-10-18 1988-10-18 Production of natural type abscisic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP26257588A JPH02109988A (en) 1988-10-18 1988-10-18 Production of natural type abscisic acid

Publications (2)

Publication Number Publication Date
JPH02109988A JPH02109988A (en) 1990-04-23
JPH0412956B2 true JPH0412956B2 (en) 1992-03-06

Family

ID=17377713

Family Applications (1)

Application Number Title Priority Date Filing Date
JP26257588A Granted JPH02109988A (en) 1988-10-18 1988-10-18 Production of natural type abscisic acid

Country Status (1)

Country Link
JP (1) JPH02109988A (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5299240A (en) * 1976-02-16 1977-08-19 Fuji Kouso Kk Edible culture medium
JPS6135838A (en) * 1984-07-28 1986-02-20 Katsuhiko Ichikawa Method and apparatus for preparing straw ash for tea ceremony
JPS63148980A (en) * 1986-12-12 1988-06-21 Okumoto Seifun Kk Production of spongy grain wastage pellet

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5299240A (en) * 1976-02-16 1977-08-19 Fuji Kouso Kk Edible culture medium
JPS6135838A (en) * 1984-07-28 1986-02-20 Katsuhiko Ichikawa Method and apparatus for preparing straw ash for tea ceremony
JPS63148980A (en) * 1986-12-12 1988-06-21 Okumoto Seifun Kk Production of spongy grain wastage pellet

Also Published As

Publication number Publication date
JPH02109988A (en) 1990-04-23

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