JPH041131A - Preventive and remedy for bacterial disease of fish - Google Patents
Preventive and remedy for bacterial disease of fishInfo
- Publication number
- JPH041131A JPH041131A JP2100430A JP10043090A JPH041131A JP H041131 A JPH041131 A JP H041131A JP 2100430 A JP2100430 A JP 2100430A JP 10043090 A JP10043090 A JP 10043090A JP H041131 A JPH041131 A JP H041131A
- Authority
- JP
- Japan
- Prior art keywords
- fish
- derivative
- pleuromutilin
- bacterial
- remedy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 17
- 208000035143 Bacterial infection Diseases 0.000 title claims abstract description 7
- 208000022362 bacterial infectious disease Diseases 0.000 title claims abstract 3
- 230000003449 preventive effect Effects 0.000 title description 4
- ZRZNJUXESFHSIO-VYTKZBNOSA-N pleuromutilin Chemical class C([C@H]([C@]1(C)[C@@H](C[C@@](C)(C=C)[C@@H](O)[C@@H]2C)OC(=O)CO)C)C[C@]32[C@H]1C(=O)CC3 ZRZNJUXESFHSIO-VYTKZBNOSA-N 0.000 claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims abstract description 6
- 208000017169 kidney disease Diseases 0.000 claims abstract description 6
- 229960002771 retapamulin Drugs 0.000 claims abstract description 4
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims abstract description 3
- 239000000126 substance Substances 0.000 claims abstract description 3
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims abstract description 3
- 229920002554 vinyl polymer Chemical group 0.000 claims abstract description 3
- 230000002265 prevention Effects 0.000 claims abstract 3
- -1 2-D-valylamino-isobutyl Chemical group 0.000 claims abstract 2
- 125000000217 alkyl group Chemical group 0.000 claims abstract 2
- 125000000623 heterocyclic group Chemical group 0.000 claims abstract 2
- 239000003814 drug Substances 0.000 claims description 9
- 229940124597 therapeutic agent Drugs 0.000 claims description 7
- 230000000069 prophylactic effect Effects 0.000 claims description 5
- 125000004103 aminoalkyl group Chemical group 0.000 claims 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
- 239000000843 powder Substances 0.000 abstract description 6
- 239000003242 anti bacterial agent Substances 0.000 abstract description 5
- 229940088710 antibiotic agent Drugs 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 5
- 150000003839 salts Chemical class 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 4
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- ZRZNJUXESFHSIO-UHFFFAOYSA-N Pleuromutilin Natural products CC1C(O)C(C)(C=C)CC(OC(=O)CO)C2(C)C(C)CCC31C2C(=O)CC3 ZRZNJUXESFHSIO-UHFFFAOYSA-N 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 3
- 239000002243 precursor Substances 0.000 abstract description 3
- 206010034133 Pathogen resistance Diseases 0.000 abstract description 2
- 241000186813 Renibacterium Species 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 abstract description 2
- 229930004069 diterpene Natural products 0.000 abstract description 2
- 150000004141 diterpene derivatives Chemical class 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 241000222350 Pleurotus Species 0.000 abstract 1
- 238000007385 chemical modification Methods 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 description 12
- 230000000694 effects Effects 0.000 description 7
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- 208000010824 fish disease Diseases 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 241000972773 Aulopiformes Species 0.000 description 4
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 235000019515 salmon Nutrition 0.000 description 4
- 235000002639 sodium chloride Nutrition 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000277331 Salmonidae Species 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 229960003276 erythromycin Drugs 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000004100 Oxytetracycline Substances 0.000 description 1
- 241000861914 Plecoglossus altivelis Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 239000002385 cottonseed oil Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- FZWBNHMXJMCXLU-BLAUPYHCSA-N isomaltotriose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)O1 FZWBNHMXJMCXLU-BLAUPYHCSA-N 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229960000625 oxytetracycline Drugs 0.000 description 1
- IWVCMVBTMGNXQD-PXOLEDIWSA-N oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 description 1
- 235000019366 oxytetracycline Nutrition 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- WTWSHHITWMVLBX-DKWTVANSSA-M sodium;(2s)-2-aminobutanedioate;hydron Chemical compound [Na+].[O-]C(=O)[C@@H](N)CC(O)=O WTWSHHITWMVLBX-DKWTVANSSA-M 0.000 description 1
- 239000001587 sorbitan monostearate Substances 0.000 description 1
- 229940035048 sorbitan monostearate Drugs 0.000 description 1
- 235000011076 sorbitan monostearate Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 description 1
- 229940126585 therapeutic drug Drugs 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はプリューロムチリン誘導体（以下、本誘導体と
いう。）を有効成分として含有することを特徴とする魚
類の細菌性疾患の予防・治療剤に関するものである。[Detailed Description of the Invention] [Field of Industrial Application] The present invention provides a prophylactic/therapeutic agent for bacterial diseases of fish, which is characterized by containing a pleuromutilin derivative (hereinafter referred to as the present derivative) as an active ingredient. It is related to.
本誘導体は日本特許：特開昭６０−１９９８７０号によ
り知られる公知の物質である。この本誘導体はプリュー
ロムチリンの半合成誘導体である。前駆物質プリューロ
ムチリンは１９５１年Ｋａｖａｎａｇｈらによりブリ二
一ロタス ムティルス（ＰｌｅｕｒｏＬｕｓ ５ｕｔｉ
ｌｕｓ）の培養ろ液から得られたジテルペン系抗生物質
であり、本誘導体は前駆物質プリューロムチリンに化学
修飾を加えて合成されたものである。This derivative is a known substance known from Japanese Patent: JP-A-60-199870. This derivative is a semi-synthetic derivative of pleuromutilin. The precursor pleuromutilin was derived from Pleurolus 5uti by Kavanagh et al. in 1951.
This derivative is a diterpene antibiotic obtained from the culture filtrate of M. lus), and this derivative was synthesized by chemically modifying the precursor pleuromutilin.
近年、養殖漁業の発達にともない、ブリ、ハマチ、ウナ
ギ、サケ、マス、アユ、コイ等々の海水ならびに淡水魚
類の養殖が盛んになり、より経済的養殖技法の開発が要
望され、同時により安全・有効な疾病の予防・治療剤の
出現が望まれている。In recent years, with the development of aquaculture, aquaculture of seawater and freshwater fish such as yellowtail, yellowtail, eel, salmon, trout, sweetfish, and carp has become popular, and there is a demand for the development of more economical aquaculture techniques, as well as safer and more efficient aquaculture techniques. The emergence of effective preventive and therapeutic agents for diseases is desired.
昨今、抗生物質を魚類に投与して魚病原因菌の抑制とそ
れに起因する疾病の予防・治療に用いて来た例は多数あ
り、オキシテトラサイクリン、アンピシリン、エリスロ
マイシン等々数多くの抗生物質は水産用薬としての用途
について知られて来ている。In recent years, there have been many cases in which antibiotics have been administered to fish to suppress fish disease-causing bacteria and to prevent and treat the diseases caused by them.Many antibiotics, such as oxytetracycline, ampicillin, and erythromycin, are used as fisheries drugs. It is becoming known for its uses.
一方では、常用される抗生物質の使用量の増加からしば
しば野性味に耐性菌の出現が見られるとの報告もあり、
なんらかの解決策が求められている。On the other hand, there are reports that due to the increase in the amount of commonly used antibiotics, resistant bacteria are often appearing in wild foods.
Some kind of solution is needed.
数多い魚種・魚病の中でも、ことにハマチの連鎖球菌症
は年々盛んになる／Ｘマチの養殖業において是非とも解
決の求められている問題であり、またしばしば認められ
る耐性菌の出現から新規治療法の考案が待望されている
。さらにサケ科魚類の細菌性腎臓病についても発生か問
題となっているが、適当な予防・治療薬が待望されてい
る。Among the many fish species and fish diseases, streptococcal disease in yellowtail is becoming more common year by year.It is a problem that desperately needs to be solved in the aquaculture industry, and due to the appearance of resistant bacteria that is often observed, new The development of a treatment method is eagerly awaited. Furthermore, the occurrence of bacterial kidney disease in salmonids has become a problem, and suitable preventive and therapeutic drugs are eagerly awaited.
本誘導体は、上記魚病の原因菌である連鎖球菌（ｓｔｒ
ｅｐｔｏｃｏｃｃｕｓ ｓｐ、）や細菌性腎臓病のレニ
バクテリウム属（Ｒｅｎｉｂａｃｔｅｒｉｕｍ ｓｐ、
）に対して１著しくすぐれた抗菌活性を示し、また従来
汎用されている各種抗生物質との間に交叉耐性が認めら
れないなどの点から、魚病の予防・治療剤として有用と
考えられる。本誘導体を魚病の予防・治療目的に使用し
たとの公知文献はない。This derivative contains streptococcus (str.
eptococcus sp,) and Renibacterium sp, which causes bacterial kidney disease.
), it is considered to be useful as a prophylactic and therapeutic agent for fish diseases, as it exhibits remarkable antibacterial activity against fish diseases and shows no cross-resistance with various conventionally used antibiotics. There is no known document that describes the use of this derivative for the purpose of preventing or treating fish diseases.
本誘導体は一般式
（式中、Ｒ１はエチルまたはビニルを表わし、Ｒ２は５
員飽和複素環または非置換もしくはアルキル部分がヒド
ロキシで置換されたアミノアルキルを表わす）
で示される化合物の遊離形、または酸付加塩、または第
４級塩であるプリューロムチリン誘導体である。This derivative has the general formula (wherein R1 represents ethyl or vinyl, R2 is 5
A pleuromutilin derivative is a free form, an acid addition salt, or a quaternary salt of a compound represented by:
特に、本誘導体のながで、特に好ましいのは１４−デオ
キシ−（（２−Ｄ−バリルアミノ−イソブチル）チオア
セトキシ〕 −ムチリンである。Particularly preferred among the derivatives is 14-deoxy-((2-D-valylamino-isobutyl)thioacetoxy)-mutilin.
本誘導体は、直接魚類に投与することもできるが、好ま
しくは、通常の抗生物質の魚への投与に見られる通り、
澱粉、ブドウ糖、デキストラン、食塩、炭酸カルシウム
、カオリン、トウモロコシ粉、大豆粕、魚粉、米ぬか、
ルーサンミール、アルコール類、油脂、綿実油、アスパ
ラギン酸ソーダ、ソルビタンモノステアレート、トウイ
ン、乳糖などの増量剤・賦形剤等々と混合して粉剤、顆
粒剤、水和剤、液剤、錠剤等々を形成して投与するか、
更に飼料は生餌に添加して投与する。Although the present derivative can be administered directly to fish, it is preferable that the derivative be administered directly to fish, but preferably,
Starch, glucose, dextran, salt, calcium carbonate, kaolin, corn flour, soybean meal, fish meal, rice bran,
Forms powders, granules, wettable powders, liquids, tablets, etc. by mixing with fillers and excipients such as Ruthen meal, alcohols, fats and oils, cottonseed oil, sodium aspartate, sorbitan monostearate, toin, and lactose. or administer
Furthermore, feed is administered by adding it to live feed.
本誘導体の投与量は魚類の種類等によって異なるが０．
１〜１０００ｐｐ■程度が適当であり、好ましくは１〜
５００ｐｐｍである。The dosage of this derivative varies depending on the type of fish, but 0.
Approximately 1 to 1000pp■ is appropriate, preferably 1 to 1000pp.
It is 500 ppm.
次に実施例を挙げて具体的に説明する。なお、本発明は
この実施例に限定されるものでないことはいうまでもな
い。Next, a concrete explanation will be given with reference to examples. It goes without saying that the present invention is not limited to this embodiment.
実施例 １ 液 剤
本誘導体１部、エチルアルコール８９．９部、ツルポー
ル（東邦化学社製品）０．１部を均一に混合して液剤と
する。Example 1 Liquid 1 part of the present derivative, 89.9 parts of ethyl alcohol, and 0.1 part of Tsurupol (manufactured by Toho Chemical Co., Ltd.) are uniformly mixed to prepare a liquid.
実施例 ２ 粉 剤（散剤）
本誘導体２部、食塩７９．９部、ツルポール（東邦化学
社製品）０．１部を均一に混合粉砕して粉剤（散剤）と
する。Example 2 Powder 2 parts of the present derivative, 79.9 parts of common salt, and 0.1 part of Tsurupol (manufactured by Toho Chemical Co., Ltd.) are uniformly mixed and pulverized to obtain a powder.
実施例 ３ 飼 料
本誘導体１０部をアセトン９０部に溶解して１００部と
し、これを幼魚用飼料（日本配合飼料株式会社製品）
９９９９００部に均一に散布混合してアセトンを除去し
て本誘導体を１０ｐｐｍ含有する飼料とする。Example 3 Feed 10 parts of the main derivative was dissolved in 90 parts of acetone to make 100 parts, and this was used as feed for young fish (product of Japan Compounded Feed Co., Ltd.).
999,900 parts of the feed were uniformly sprinkled and mixed to remove acetone to obtain a feed containing 10 ppm of this derivative.
実施例 ４ 飼 料
本誘導体０．２部を大豆粕の粉９９．８部に均一に混合
粉砕して１００部とし、これを魚子用飼料（日本配合飼
料株式会社製品） ９９００部に均一に混合して本誘導
体を２０ｐｌ）１ｍ含有する飼料とする。Example 4 Feed 0.2 part of the main derivative was uniformly mixed and pulverized with 99.8 parts of soybean meal powder to make 100 parts, and this was uniformly mixed with 9900 parts of fish feed (product of Japan Compounded Feed Co., Ltd.). A feed containing 20 pl) 1 m of this derivative is prepared.
次に本誘導体を用いて魚類の細菌性疾患の予防・治療効
果を試験例を挙げて具体的に説明する。Next, the preventive and therapeutic effects of bacterial diseases in fish using this derivative will be specifically explained using test examples.
試験例 １
薬剤をメタノールまたは蒸留水に溶解して、順次希釈し
、１００ｘ／ｍｌ、 ５０部ｇ／ｍ１． ５ｔｔｇ／ｍ
ｌおよびｌｕｇ／ｍｌの各濃度における抗菌活性を検討
した。Test Example 1 A drug was dissolved in methanol or distilled water and diluted sequentially to 100x/ml and 50 parts g/ml. 5ttg/m
The antibacterial activity at each concentration of lug/ml and lug/ml was investigated.
すなわち直径８關のペーパーディスクを上記希釈液に浸
漬し、クリーンベンチ内で１時間風乾後、魚病菌０．１
ｍｌをコンラージを用いて接種（＊１）した寒天培地（
＊２）上に置き、培養（＊３）後抗菌性の有無を阻止円
形成の有無を以て判定した。That is, a paper disk with a diameter of 8 mm was immersed in the above diluted solution, and after air-drying in a clean bench for 1 hour, 0.1
ml of agar medium (*1) inoculated using Conlage (
*2) After culturing (*3), the presence or absence of antibacterial properties was determined by the presence or absence of inhibition circle formation.
＊１）接種菌量 １０８ｃＵＦ
＊２）判定用培地 感性ディスク用培地＊３）培養条
件 ２６．５℃４０時間
結果は効果大（＋１＋）、効果中（＋）、効果中ωおよ
び効果なし０で表わし、次表に示す。*1) Inoculum amount 108 cUF *2) Judgment medium Sensitive disk medium *3) Culture conditions 26.5℃ 40 hours The results are: large effect (+1+), medium effect (+), medium effect ω, and no effect 0. The expression is shown in the table below.
注）ル、１＋１４−デオキシ−（（２−Ｄ−バリルアミ
ノ−イソブチル）チオアセトキシ〕 −ムチリンＮＤ、
２：アンピリシン
陥３：エリスロマイシン
＊ ：ハマチの連鎖球菌症の病原菌
試験例 ２ ハマチの連鎖球菌症に対する効果１区１０
尾のハマチの筋肉内にハマチの連鎖球菌の生菌（１尾当
り１０７個）を感染させた直後から本誘導体Ｎｏ、１（
前記のとおり） １ ｐｐｍ、 ５ ｐｐｍ。Note) 1+14-deoxy-((2-D-valylamino-isobutyl)thioacetoxy)-mutilin ND,
2: Ampicillin 3: Erythromycin *: Pathogen test for streptococcosis in yellowtail 2. Effect on streptococcus in yellowtail 1 area 10
This derivative No. 1 (
(as above) 1 ppm, 5 ppm.
１００ｐｐＩＩｌを含む水［（１００４！ ）に入れ、
感染魚をＩＯ日間連続薬浴した結果、非薬浴感染対照区
は感染部位に壊死症状を呈し感染後２週間以内に全部死
亡したが、本誘導体１ｌｌＱ、１による薬浴区は発病も
認められず全部生存した。Water containing 100 ppIIl [(1004!)]
As a result of continuous medicinal bathing of infected fish for 10 days, the non-medicated infected control group showed necrotic symptoms at the infected site and all died within 2 weeks after infection, but the fish treated with this derivative 1llQ, 1 did not develop disease. All survived.
試験例 ３ サケの細菌性腎臓病に対する効果１区２０
尾のサケを用い、その各区に１０尾の細菌性腎臓病に感
染したサケを入れ、本誘導体Ｎ０１１（前記のとおり）
２ｐｐｍを含をする餌料を１５日間与え、１ケ月後の生
存数を検査した結果、本誘導体０．２部加の区は８０％
の生存率を示したのに対し、無添加区は８５％死亡した
。Test example 3 Effect of salmon on bacterial kidney disease Group 1 20
Using tail salmon, each section contained 10 salmon infected with bacterial kidney disease, and the present derivative N011 (as described above) was prepared.
Feed containing 2 ppm was given for 15 days, and after one month, the number of survivors was examined. As a result, 80% of the rats added 0.2 parts of this derivative.
The survival rate was 85% in the non-additive group.
以上により本誘導体は魚類の細菌性疾患の予防・治療剤
として効果があることがわかる。The above shows that this derivative is effective as a prophylactic and therapeutic agent for bacterial diseases in fish.
Claims (3)
は５員飽和複素環または非置換もしくはアルキル部分が
ヒドロキシで置換されたアミノアルキルを表わす） で示される化合物の遊離形、または酸付加塩、または第
４級塩であるプリューロムチリン誘導体を有効成分とし
て含有することを特徴とする魚類の細菌性疾患の予防・
治療剤。(1) General formula ▲ There are mathematical formulas, chemical formulas, tables, etc. ▼ (In the formula, R_1 represents ethyl or vinyl, R_2
represents a 5-membered saturated heterocycle or an aminoalkyl group in which the alkyl moiety is unsubstituted or substituted with hydroxyl. Prevention and prevention of bacterial diseases of fish characterized by containing
therapeutic agent.
病である請求項１記載の予防・治療剤。(2) The prophylactic/therapeutic agent according to claim 1, wherein the bacterial disease of fish is streptococcosis and bacterial kidney disease.
４〔（２−Ｄ−バリルアミノ−イソブチル）チオアセト
キシ〕−ムチリンである請求項２記載の予防・治療剤。(3) The pleuromutilin derivative is 14-deoxy-1
The prophylactic/therapeutic agent according to claim 2, which is 4[(2-D-valylamino-isobutyl)thioacetoxy]-mutilin.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2100430A JPH041131A (en) | 1990-04-18 | 1990-04-18 | Preventive and remedy for bacterial disease of fish |
| CA 2042138 CA2042138A1 (en) | 1990-04-18 | 1991-05-08 | Agents for preventing or treating bacterial diseases of fishes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2100430A JPH041131A (en) | 1990-04-18 | 1990-04-18 | Preventive and remedy for bacterial disease of fish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH041131A true JPH041131A (en) | 1992-01-06 |
Family
ID=14273740
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2100430A Pending JPH041131A (en) | 1990-04-18 | 1990-04-18 | Preventive and remedy for bacterial disease of fish |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH041131A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110352879A (en) * | 2019-08-07 | 2019-10-22 | 广西壮族自治区水产科学研究院 | A kind of method for breeding tilapia of prevention and control Tilapia mossambica streptococcosis |
-
1990
- 1990-04-18 JP JP2100430A patent/JPH041131A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110352879A (en) * | 2019-08-07 | 2019-10-22 | 广西壮族自治区水产科学研究院 | A kind of method for breeding tilapia of prevention and control Tilapia mossambica streptococcosis |
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