JPH0399245A

JPH0399245A - Viscometer

Info

Publication number: JPH0399245A
Application number: JP23466889A
Authority: JP
Inventors: Hiroyoshi Moriyama; 浩義森山; Toshihiro Ishii; 石井　敏浩
Original assignee: Individual
Current assignee: Individual
Priority date: 1989-09-12
Filing date: 1989-09-12
Publication date: 1991-04-24

Abstract

PURPOSE:To simplify the insertion and removal of a tube and washing by forming the tube of a viscometer as a vertical tube having a simple structure, and forming the lower part of the tube as a thin tube which is suitable for sucking and dropping liquid. CONSTITUTION:For example, liquid which is made to react with enzyme and a substrate in a test tube and has viscosity is sucked through the tip of a thin, long tube 3 at the lower part of a main body 2 of a vertical tube by imparting negative pressure with a rubber tube 13. The falling time of the liquid from an upper scale 14 to a lower scale 15 is measured. The size of the slender tube 3 at the lower part is determined so that the tube is suitable for sucking and dropping the liquid and the liquid having high viscosity can be measured.

Description

【発明の詳細な説明】（産業上の利用分野〉本発明は酵素と基質を反応させた粘性のある液体の落下する時間を測定する粘度計に関するものである。[Detailed description of the invention] (Industrial application field) The present invention is based on a viscous membrane that is made by reacting an enzyme with a substrate. Measuring the time it takes for a liquid to fall The present invention relates to a viscometer.

（従来の技術〉従来の技術に番よ歴史白勺番こも実際白勺Ｇ二も最も基
本的なものである垂直形毛管粘度計であるオストワルト粘度計（第８図）及びオストワルト粘度計の変形改良形である懸垂液面形のウベローデの粘度計（第９図）があるーオストワルト粘度計は〜標準物質例えば水の粘度を基としてこれを比較した値を実測することを可能にしたものである。(Conventional technology) Turn to the conventional technology.History. There is an improved version of Ubbelohde's viscometer (Fig. 9), which uses a suspended liquid level.The Ostwald viscometer makes it possible to actually measure values based on the viscosity of a standard substance, such as water. be.

次にウベローデの粘度計は高分子希薄溶液の固有粘度の決定のためによく用いられるものであり〜オストヮルト粘度計では液量が増すと有効落差が変わるので不便であるがーウベローデの粘度計の有効落差は毛管長に比し〜上球を小さくしておけばほとんど一定であるという特徴を持っている。Next, Ubbelohde's viscometer Well for determination of intrinsic viscosity of thin solutions It is used ~ Ostwort With a viscometer, the effective head changes as the liquid volume increases. Although it is inconvenient because the The effective head of the viscometer is ~ higher than the capillary length. If the ball is made small, it will remain almost constant. It has the characteristic of being.

（発明が解決しようとする課題〉前記した従来の粘度計はいずれも構造が複雑でＵ字型管であるため一出し入れが不便一洗浄が不便であり一又管の内で１回１回酵素と基質を反応させなＣナれｃスならなｂ）力）ら時間カイカ）力）り一更
に反応の温度を一定に保つために一恒温槽が必要でありーこの恒温槽に入れて垂直に固定する場合恒温槽の上に出ている部分をクリップ等で止めなければならず所定位置に垂直に固定するの６こｙｈ常に時間力｛力）力）り〜　手間カイヵ）力
）ったーそして毛細管を通して落下する時間を測定するので〜粘度が高いとな力）な力）落ちてこす時間力｛力）力）りすぎて使用に
適さないこと等の問題点があった。(Problems to be Solved by the Invention) All of the conventional viscometers described above have a complicated structure and are U-shaped tubes, making it inconvenient to take them in and out and cleaning them. In order to keep the temperature of the reaction constant, a constant temperature bath is required. When fixing it to a thermostatic chamber, the part protruding above the thermostatic chamber must be fixed with clips, etc., and it takes 6 hours to fix it vertically in a predetermined position. Since the time it takes for the liquid to fall through a capillary tube is measured, there are problems such as when the viscosity is high, the amount of time it takes to fall is too long, making it unsuitable for use.

本発明は従来技術の有するこのような問題点に鑑みてなされたものでありその目的とするところは管の出し入れ一洗浄を簡単にし一管の内で酵素と基質を反応させる必要をなくし一又床に対し垂直に固定するのを容易かつ簡単番こし一更に粘度の高し）液体でも浪リ定することが
出来るようにすることであり一他の目白勺６よ恒温槽を必要とせず〜管の内で酵素
と基質を反応させることを可能とし〜管本体の温度を一定に保たせることにより正確に測定値を出せるようにしたことである。The present invention has been made in view of the above-mentioned problems of the prior art, and its purpose is to simplify the loading and unloading of tubes and cleaning, eliminate the need for reacting enzymes and substrates in one tube, and It is easy and simple to fix perpendicularly to the floor, and it is also possible to fix even liquids (with high viscosity), and unlike other Mejiroki 6, it does not require a constant temperature bath. This makes it possible to cause the enzyme and substrate to react within the tube, and by keeping the temperature of the tube body constant, it is possible to obtain accurate measured values.

〈課題を解決するための手段〉上記目的を達戒するため一本発明における粘度計は一上部をやや細めの管にし一下部の管を液体を吸いあげたり落下させたりするのに適した細長い管にし一中央部を上部及び下部の管よりも太めの大きさの管にした垂直管型にし〜別のもう一つは一一定に保たれた温度の水を流入一流出する管をそれぞれ上部一下部に設けた管本体の内部に前記垂直管を内包したことからなるものであるーそしてーこれらの粘度計の本体に粘度計支持板をカン合し一更にその支持板に水準計を固着したーく作用）本発明は管を構造の簡単な垂直管にしたので出し入れ〜洗浄が容易となり又粘度計本体に粘度計支持板をカン合させ一鉄製等のスタンドに磁気着させると床に対して垂直に固定することが容易となる。〈Means for solving problems〉 In order to achieve the above purpose, we have developed this invention. The top of the viscometer is a slightly narrow tube. Then suck up the liquid through the tube at the bottom. A long thin tube suitable for dropping Then, insert the center part from the upper and lower tubes. Vertical tube type with a thicker size tube Shi~ Another one was kept constant. Temperature water inflow and outflow tubes respectively Inside the tube body provided at the top and bottom A tube made by enclosing the above-mentioned vertical tube - and - these viscometers Attach the viscometer support plate to the main body and then I fixed a level gauge to the support plate. action) The present invention transforms the pipe into a vertical pipe with a simple structure. This makes it easy to put in and take out and clean. Also, attach the viscometer support plate to the viscometer body. Attach magnetically to a stand made of steel or similar material. When placed, it must be fixed perpendicular to the floor. becomes easier.

又垂直管を内包する管本体を設けたので〜この管に恒温槽に汲みこまれている一定の温度の水をポンプで下の管から流入させーその一定の温度の水を上の管を通して恒温槽に流出させ〜又更６こその温水をボンフ゜で下の管力）ら管本体番こ流
入させるとり）う具合にその一定の温度の水を循環させ
ることにより温度を一定に保っこと力く出来一前言己液体の落下時間
を正確に測定することが出来ることになる。In addition, since a tube body containing a vertical tube was provided, the water at a constant temperature, which had been pumped into the thermostatic chamber, was pumped into this tube from the lower tube, and the water at a constant temperature was passed through the upper tube. The temperature can be kept constant by circulating the water at a constant temperature as shown in Figure 6. This makes it possible to accurately measure the falling time of the liquid.

そして垂直管の下部を前記液体を吸し）あＧデたり落下させたりするの番こ適した細さの管
にした。The lower part of the vertical tube was made into a thin tube suitable for sucking up and dropping the liquid.

更に粘度計支持板に水準計を設けたので一床に対して垂直に固定されてぃるか否かを正確に把握することが出来る。Additionally, a level gauge was installed on the viscometer support plate. Therefore, it is fixed perpendicularly to the floor. It is possible to accurately determine whether Ru.

（実施例〉実施例について図面を参照して説明するー第１図におり）て〜垂直管本体２ｃよ〜酵素と基質を反
応させた粘性のある液体が落下するのが〜肉眼で見えるように透明の円形の管であることが好ましい。しかし〜多角形の管でもよいー細長い下部の管３の先端よりーたとえば試験管の内で酵素と基質を反応させた粘性のある液体をーゴムチューブ１３により陰圧を与
えて吸いあげ一上の目盛１４から下の目盛１５まで落下する時間を測定するの
である。したがって細長し）下部の管３ζよ〜前言己液体を吸し）上Ｃヂ又落下
させるのに適し〜さらに粘度の高い液体でも測定可能な幅の細さであることが必要であり〜そのため毛細管よりやや太めである。(Example) An example will be explained with reference to the drawings (see Figure 1). From the vertical tube body 2c, it is visible to the naked eye that a viscous liquid made by reacting an enzyme and a substrate falls. It is preferably a transparent circular tube. However, a polygonal tube may also be used. From the tip of the elongated lower tube 3, for example, a viscous liquid obtained by reacting an enzyme and a substrate in a test tube is sucked up by applying negative pressure to the rubber tube 13, and the upper scale is drawn up. The time it takes to fall from 14 to the lower scale 15 is measured. Therefore, the lower tube 3ζ is elongated (slender), and the upper tube (3ζ) is suitable for sucking up the liquid and letting it fall again. Furthermore, it needs to be narrow enough to be able to measure liquids with high viscosity. Therefore, the capillary tube It is slightly thicker.

第３．４図は本発明の垂直管型の粘度計及びそれを内包した粘度計に粘度計支持板１０をカン合させた状態の図であり〜その支持板
１０の一側部９が磁気を帯しているので〜その一側部９
に鉄製等で出来たクランプ８を有するスタンド１２（第５図）のそのクランプに磁気着させると床に
対して垂直に固定することカイプ１常に容易力）つ簡単６こ出来るーなお垂直
に固定できればスタンドでなくても鉄等の垂直の壁を使用しても良％）．前言己支持板１０Ｌ！＝多角形〜好適番二Ｃ−！
．四角形が良いが（第６図〉円形でも良ｂ）さらに前言己支持板１０　Ｇ．：　Ｌ！：一水準計１１
力｛設けてあるので〜粘度計が床に対して垂直に固定されているかどうかを正確にみることができる。Figure 3.4 is a diagram of the vertical tube type viscometer of the present invention and the viscometer containing the viscometer in which the viscometer support plate 10 is fitted. One side 9 of the support plate 10 is magnetically Because it has a belt ~ One side part 9
By magnetically attaching the clamp 8 of a stand 12 (Fig. 5) made of iron or the like to the clamp, it can be fixed vertically to the floor. If possible, you can use a vertical wall made of steel instead of a stand). Maego self support board 10L! = Polygon ~ Suitable number 2C-!
．． A rectangular shape is best (Fig. 6) A circular shape is also acceptable.) In addition, the previous self-supporting plate 10G. : L! :One level gauge 11
Since the force is provided, it is possible to accurately check whether the viscometer is fixed perpendicular to the floor.

第２図において一管本体１には恒温槽で一定に保たれた温度の水をーボンブで汲み入れるための管５が下部に設けられーその管５を通って流入した前記水がボンブの勢いで上昇し一管６を通って流入した前言己水力＜７Ｈンフーの勢ｂ）で上昇し
一管６を通って恒温槽へ流出しだらに恒温槽にたまった前記水をポンプが汲み出し一管５に流入させるという具合に循環させ（図示せず〉管本体１の温度を一定に保つことが出来るようにしてある。又一外で反応させた場合一その外で反応させた温度に管本体１の内の温度を一定に保つことが出来一これにより垂直管の内で酵素と基質を反応させることが出来一落下時間を正確に測定することができる。In FIG. 2, a tube body 1 is provided with a tube 5 at the bottom for pumping water at a constant temperature in a constant temperature bath with a bomb, and the water flowing through the tube 5 is pumped by the force of the bomb The pump pumps out the water, which rises with the force b) of water force < 7H and flows through the first tube 6, and flows out through the first tube 6 into the constant temperature bath, which accumulates in the constant temperature bath. 5 (not shown) so that the temperature of the tube body 1 can be kept constant.Also, when the reaction is carried out outside, the temperature of the tube body 1 is maintained at the same temperature as that of the outside. The temperature inside the tube can be kept constant, which allows the enzyme and substrate to react in the vertical tube, and allows for accurate measurement of fall time.

（発明の効果〉本発明は以上に説明したように構或されているので一粘度計の出し入れ一後又すぐ洗浄して使用可能となり〜又管の内で一回一回酵素と基質を反応させる必要がないため一短時間で多数の液体の落下時間を測定することが可能となり，また構造が簡単なので製造コストも安くできるーさらに〜粘度計を床に対して垂直に固定することが容易となり一手間がかからず便利となったー又一恒温槽に粘度計を入れる必要がないため扱いが簡単であり一特に第２図に示された粘度計を使用すると〜落下時間を正確に測定することが出来るーしたがって本発明の粘度計は使用するの力′ｃ−Ｊｔａ常にイ更オリで簡単であるとし）う
大きな利点を有するものであるー(Effects of the Invention) Since the present invention is constructed as described above, it is possible to use the viscometer by washing it immediately after putting it in and taking it out. Also, the enzyme and substrate can be reacted one by one in the tube. Since there is no need to move the viscometer vertically to the floor, it is possible to measure the falling time of a large number of liquids in a short period of time, and the simple structure reduces manufacturing costs.Furthermore, it is easy to fix the viscometer perpendicular to the floor. This is very convenient and requires no effort. Also, it is easy to handle because there is no need to put a viscometer in a constant temperature bath. Especially when using the viscometer shown in Figure 2, it is possible to accurately measure the falling time. The viscometer of the invention therefore has the great advantage of being able to measure the forces used - which are always the best and simplest.

[Brief explanation of drawings]

第１図は本発明に係る垂直管型の粘度計をあらわす図であるーわす図である− 第３．４図は支持板を固着した本発明の粘度計をあらわす図であるー第５図は本発明の粘度計を床に対して垂直に固定するためのスタンドをあらわす図である。第６図は本発明の支持板をあらわす図である。第７図は従来例のオストワルト粘度計を〜第８図は従来例のウベローデの粘度計をあらわす図である。１−・−−一管本体２−一一一・垂直管本体３−　−　−−一下部の管３　ａ−　−−一上部の管３　ｋ：）−　−−一中央部の管４　，　４　ａ−　　栓５・−−−一流入管６−一・一一流出管８−−−−−クランフ゜糸１凹力ス圏　　　　　力３１！！一２６０ー磁気を有する一側端部 −事占度言十支持板 −水準計 −スタンド・ゴムチューブ一上の目盛一下の目盛一穴部 FIG. 1 shows a vertical pipe type viscosity according to the present invention. This is a diagram showing a meter. This is a diagram. Figure 3.4 shows the main engine with the supporting plate fixed. This is a diagram showing a light viscometer. Figure 5 shows the viscometer of the present invention placed on the floor. Add a stand to fix it vertically. This is a diagram showing the details. Figure 6 shows the support plate of the present invention. It is a diagram. Figure 7 shows the Ostwald viscosity of the conventional example. Figure 8 shows the conventional example of Ubbelohde. It is a figure showing a viscometer. 1-・--One tube body 2-11/Vertical tube body 3-----One lower tube 3 a--- Upper tube 3k:)---One central tube 4, 4 a- Plug 5.---1 inflow pipe 6-1/1st outlet pipe 8------ Clamp゜ thread 1 concave Power sphere Power 31! ! -260 - One end with magnetism -Jiji divination support board -Level gauge −Stand ・Rubber tube First scale scale below One hole part

Claims

[Claims] 1. The upper part is a slightly narrow tube (3a), the lower part is a long and thin tube (3) suitable for sucking up or dropping liquid, and the central part is a tube (3b). This is a vertical tube type viscometer that is thicker than the upper tube (3a) and the lower tube (3). 2. A stopper (4) is provided at the bottom of the tube body (1), and a pipe (5) is provided in the stopper (4) through which water at a constant temperature from a constant temperature bath flows; ) is provided with a stopper (4), a pipe (6) is provided on the stopper (4a) through which water at a constant temperature flows out from the constant temperature bath, and the pipe body (1) is provided with the vertical pipe type. The viscometer is penetrated through the upper tube (3a), the lower tube (3) and the stopper (4, 4a). 3. A polygonal viscometer support plate (10) having a hole (16) that fits into the main body of the viscometer according to claim 1 or 2, and having magnetism at one end (9). . 4. The viscometer support plate (10) according to claim 3, wherein the viscometer support plate (10) is circular. 5. The viscometer according to claim 3 or 4, wherein the viscometer support plate (10) is provided with a level gauge (11).