JPH0387164A - Method for grinding cell membrane of alga such as chlorella - Google Patents

Abstract

PURPOSE:To obtain the title algae having high commercial value in extremely reduced production of pheohorbide by subjecting slurry of single cell grouped body containing chlorophyll to a specific treatment, grinding cell membrane thereof with a grinder and removing water content from the ground cell with spray drier. CONSTITUTION:Temperature-controlled slurry obtained by controlling the temperature of chlorophyll-containing single cellular slurry (e.g. chlorella) in water to <=5 deg.C in a temperature controlling tank 1 is heated to high temperature (110-120 deg.C) at a short time (within 10sec.) by an instant heating device 2 to give high-temperature slurry, which is then cooled to 5 deg.C by a cooling device 7 to afford a cooled slurry. The cooled slurry is ground with a grinder 9 such as pearl mill to afford ground slurry by destructing the cell membrane. Water content is removed to about 85% from the ground slurry using a spray drier 12 to provide the objective algae.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing crushed chlorella, etc., which produces very little or almost no toxic 7-eophobide.

[Conventional technology]

Regarding the conventional manufacturing method of crushed chlorella,
There are things like 2351. This conventional technology is described in the claims and is as follows.

A suspension of chlorella powder and water with a chlorella concentration of 10 to 25% by weight was adjusted to below 10°C, and a large number of glass beads with a diameter of 0.5 to 5 rnn were completely enclosed in a closed cylinder with a cooling jacket. The glass bead volume is 80-85% of the closed cylinder capacity, and the glass beads are mixed with the inflow liquid and the suspension is placed in a continuous wet pulverizer which grinds the material in the inflow liquid by 0 revolutions. The method is characterized in that the slurry immediately after crushing is pulverized to a temperature of 40° C. or lower by feeding the liquid, and after cooling the slurry to a temperature of 10° C. or lower, a drying step is performed.

[Problem to be solved by the invention]

In the conventional method for disrupting chlorella cell membranes, the cell membranes are disrupted, but as an incidental phenomenon, almost all of the chlorophyllase activity, which is 400 to 1400 mg%, is destroyed by toxic substances. There was a problem in that it changed into a Hueohornoid, which could be said to be a. In other words, the products produced by this conventional crushing method are
Eating this product causes photosensitivity and other effects, making it impossible to use it as food. Furthermore,
Conventional products made using this method have problems such as brown moldiness and decreased commercial value.

By the way, the Ministry of Health and Welfare's guidance standards for chlorella are that pheo-7orbide must be 100 mg% or less, and the sum of pheophorbite and chlorophyllase activity must be 160 mg%.
It is as follows.

The present invention has extremely high digestibility, produces almost no pheophorbite, is fully compliant with the guidance standards of the Ministry of Health and Welfare, and can be safely used as a nutritional food.
The purpose is to obtain a method for producing crushed chlorella etc. which can highly exhibit the health maintenance effect of the original chlorella and furthermore does not lose the bright green color peculiar to chlorella.

[Means to solve the problem]

In order to achieve the above object, the present invention prepares a slurry of chlorophyll-containing single cell colonies such as chlorella in water to a temperature of about 5° C. or lower in a temperature-controlled tank to obtain a low-temperature slurry,
This low-temperature slurry is introduced into an instantaneous heating device to raise the temperature to a high temperature, for example about 110° C., in a very short time, for example within 10 seconds.
The slurry is heated in a trench to about 120°C to form a high-temperature slurry, and this high-temperature slurry is led to a cooling device and cooled to about 5°C to form a cold slurry. The cell membranes are crushed to form a crushed slurry, and this crushed slurry is introduced to a spray dryer to remove water. Note that the purpose of raising the temperature to a high temperature is to completely inactivate the chlorophyllase enzyme in the temperature-controlled slurry, so the temperature is not necessarily limited to the above temperature as long as it can be deactivated.

[Effect]

When a chlorophyll-containing body is gently heated, the chlorophyllase enzyme acts on the chlorophyll, converting the chlorophyll into the toxic 7-eofluoride. - In the present invention, the temperature of this chlorophyllase enzyme is instantaneously raised to a temperature outside the action temperature range, and at the same time, the high temperature is used to thermally destroy the enzyme and deactivate its activity. In order to deactivate the chlorophyll, the crushing treatment is carried out without giving any time to act on the chlorophyll.

Cooling after crushing prevents secondary decomposition of chlorophyll due to the small amount of chlorophyllase activity remaining.

〔Example〕

Examples will be described with reference to the drawings.

A chlorophyll-containing single cell colony such as chlorella is made into a slurry with water, and the temperature is controlled to below about 5° C. and stored as a low-temperature slurry in a temperature-controlled tank 1. The solid content of chlorella or the like in the low-temperature slurry is, for example, 15%.

The valve 13 opens and closes the outlet of the temperature-controlled slurry.

The low-temperature slurry is led to the instantaneous heating device 2 and heated to about 110°C to 120°C in a very short time, for example within about 10°C.
It is heated to a high temperature of about ℃ to form a high-temperature slurry.

As the instant heating device 2, for example, a plate heater can be used. The low-temperature slurry is fed into the instant heating device 2 at a rate of, for example, about 1 to 3 kg/min by a Mono pump 3 or the like. The processing flow rate is adjusted by valves 4 and 5. The slurry in the instantaneous heating device 2 is forced to circulate by a gear pump, rotary pump, etc., causing turbulence and promoting an instantaneous temperature rise.

The forced circulation is, for example, 40 to 80 liters/minute.

The high-temperature slurry is led to a cooling device 7 through a valve 4 and cooled to about 5° C. or lower to become a cold slurry. The cold/hot slurry is guided by a Mono pump 8 to a crushing device 9 such as a Cipal mill or a Dyno mill, where the cell membranes are crushed and a crushed slurry is obtained. This crushed slurry is temporarily stored in a cooling cushion tank 10 to form a slurry at about 5°C. This slurry is introduced into a plunger pump 11 and a system blade dryer 12 to remove about 85% of the moisture to produce a crushed powder, that is, a product.

The data obtained in this example are approximately as follows.

A RiCIL/raw material 15 Normal concentration 1 Product data of pearl mill processing speed 1 liter/min Pheophorbite 20ry% (raw material is 20 md) Chlorophylase activity 0 Tomobi (raw material is 800 mg%) 2 Pearl mill processing Speed 3 liters/min Product data Pheophorbite -55mg% (Raw material is 20 rrd
) Chlorophylase activity 1DDmd ← Raw materials are 8
D D rrrz% ) B In the case of 10% concentration of chlorella raw material, almost the same residue as in the case of 15% concentration was obtained.

〔Effect of the invention〕

Since the present invention is a method as described above, it produces effects as described below.

It solves the problems of conventional methods, has extremely high digestibility, produces extremely little pheophorbite, fully meets the guidance standards of the Ministry of Health and Welfare, and can be safely used as a nutritional food. A method for disrupting cell membranes of chlorella, etc. can be obtained, which can highly exert the health-maintaining effect of chlorella, and can also increase the commercial value by maintaining the bright green color unique to chlorella.

[Brief explanation of drawings]

The figure is a system diagram of an embodiment of the present invention. In the figure, the temperature control tank 2: Instant heating device 3.8: Mono pump 4.5.13: Valve 6.11: Pump 7: Cooling device 9: Crushing device 10=Cooling cushion tank 12 Nispray dryer

Claims (1)

[Claims] 1. A water slurry of a chlorophyll-containing single cell colony such as chlorella is heated to a temperature of about 5°C or less in a temperature-controlled tank to obtain a temperature-controlled slurry, and this temperature-controlled slurry is introduced into an instantaneous heating device to heat the slurry in a very short period of time, for example, 10°C. Approximately 110℃ for about seconds
The slurry is heated to ~120°C to form a high-temperature slurry, the high-temperature slurry is led to a cooling device and rapidly cooled to form a cold slurry, and the cold slurry is led to a crushing device such as a pearl mill to crush the cell membranes to create a crushed slurry. A method for disrupting cell membranes of algae such as chlorella, which is characterized by introducing this crushed slurry into a spray dryer to remove its water content.