JPH0387164A - Method for grinding cell membrane of alga such as chlorella - Google Patents
Method for grinding cell membrane of alga such as chlorellaInfo
- Publication number
- JPH0387164A JPH0387164A JP1224295A JP22429589A JPH0387164A JP H0387164 A JPH0387164 A JP H0387164A JP 1224295 A JP1224295 A JP 1224295A JP 22429589 A JP22429589 A JP 22429589A JP H0387164 A JPH0387164 A JP H0387164A
- Authority
- JP
- Japan
- Prior art keywords
- slurry
- temperature
- chlorella
- ground
- chlorophyll
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000195649 Chlorella <Chlorellales> Species 0.000 title claims abstract description 20
- 210000000170 cell membrane Anatomy 0.000 title claims abstract description 10
- 238000000034 method Methods 0.000 title claims description 7
- 239000002002 slurry Substances 0.000 claims abstract description 46
- 229930002875 chlorophyll Natural products 0.000 claims abstract description 11
- 235000019804 chlorophyll Nutrition 0.000 claims abstract description 11
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 claims abstract description 11
- 238000001816 cooling Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 210000004027 cell Anatomy 0.000 claims abstract description 5
- 239000007921 spray Substances 0.000 claims abstract description 4
- 241000195493 Cryptophyta Species 0.000 claims abstract 3
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000001413 cellular effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 11
- 108010025790 chlorophyllase Proteins 0.000 description 6
- 239000002994 raw material Substances 0.000 description 6
- 239000011324 bead Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 235000019621 digestibility Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010034972 Photosensitivity reaction Diseases 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000036211 photosensitivity Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Landscapes
- Seeds, Soups, And Other Foods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【発明の詳細な説明】
本発明は、毒性7エオフオバイドの生成することの極め
て少ないか又はほとんど皆無の破砕クロレラ等の製法に
関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing crushed chlorella, etc., which produces very little or almost no toxic 7-eophobide.
従来の破砕クロレラの製法にあっては、特公昭55−3
2351の如きものがある。この従来の技術は、その特
許請求の範囲に記載されているものであって、次のよう
なものである。Regarding the conventional manufacturing method of crushed chlorella,
There are things like 2351. This conventional technology is described in the claims and is as follows.
クロレラ濃度10〜25重量優のクロレラ粉体、水懸濁
液を10℃以下に調整し、冷却外套を持つ密閉シリンダ
ー中に多数の直径0.5〜i、5rnnのグラスビーズ
全封入して該グラスビーズ容量が密閉シリンダー容量の
80〜85%になる何くし前記グラスビーズを流入液体
と混和0回転することによシ、流入液体中の物質を摩砕
する連続湿式微粉砕機に前記懸濁液を送入して、破砕直
後のスラリーが40℃以下になる如く微粉砕し、スラリ
ー温度を10℃以下になる如く冷却した後、乾燥工程を
経ることに特徴とするものである。A suspension of chlorella powder and water with a chlorella concentration of 10 to 25% by weight was adjusted to below 10°C, and a large number of glass beads with a diameter of 0.5 to 5 rnn were completely enclosed in a closed cylinder with a cooling jacket. The glass bead volume is 80-85% of the closed cylinder capacity, and the glass beads are mixed with the inflow liquid and the suspension is placed in a continuous wet pulverizer which grinds the material in the inflow liquid by 0 revolutions. The method is characterized in that the slurry immediately after crushing is pulverized to a temperature of 40° C. or lower by feeding the liquid, and after cooling the slurry to a temperature of 10° C. or lower, a drying step is performed.
従来のこの特公昭55−32351クロレラ細胞膜の破
砕方法にあっては、細胞膜は破砕されるが、必らずそれ
に伴う付帯現象として、400〜1400 mg%もあ
るクロロフィラーゼ活性が、殆ど全部、毒物であるとい
ってよいフエオフオルノくイドに変化してしまう問題点
があった。すなわちこの従来の破砕方法による製品は、
これを食すると光過敏症、その他の影響があって、食品
に供することができない問題点があった。さらにまた、
従来のこの方法による製品は、茶色ヲかび、商品価値が
下がる問題点があった。In the conventional method for disrupting chlorella cell membranes, the cell membranes are disrupted, but as an incidental phenomenon, almost all of the chlorophyllase activity, which is 400 to 1400 mg%, is destroyed by toxic substances. There was a problem in that it changed into a Hueohornoid, which could be said to be a. In other words, the products produced by this conventional crushing method are
Eating this product causes photosensitivity and other effects, making it impossible to use it as food. Furthermore,
Conventional products made using this method have problems such as brown moldiness and decreased commercial value.
ちなみに、厚生省のクロレラに関する指導規準は、フェ
オ7オルバイド100mg%以下であシ、フェオフォル
バイトとクロロフィラーゼ活性との総和は160mg%
以下である。By the way, the Ministry of Health and Welfare's guidance standards for chlorella are that pheo-7orbide must be 100 mg% or less, and the sum of pheophorbite and chlorophyllase activity must be 160 mg%.
It is as follows.
本発明は、消化率が極めて高く、フェオフォルバイトの
生成が皆無かそれに近く、厚生;省の指導規準に充分適
格であり、安心して栄養食品とし役立てることができ、
本来のクロレラの持つ健康維持の効果を高度に発揮する
ことのできると共に、更にはクロレル特有の鮮明なる緑
色を失わせることのない破砕クロレラ等の製法を得るこ
とをもってその目的とする。The present invention has extremely high digestibility, produces almost no pheophorbite, is fully compliant with the guidance standards of the Ministry of Health and Welfare, and can be safely used as a nutritional food.
The purpose is to obtain a method for producing crushed chlorella etc. which can highly exhibit the health maintenance effect of the original chlorella and furthermore does not lose the bright green color peculiar to chlorella.
上記目的を達成するために、本発明は、クロレラの如き
クロロフィル含有単細胞群体の水によるスラリーを調温
タンク中にて約5℃以下に調温して低温スラリーとし、
この低温スラリーを瞬間加熱装置に導いて極めて短時間
に、例えば10秒間以下に、高温に、例えば約110℃
〜120℃程度に壕で加熱して高温スラリーとし、この
高温スラリーを冷却装置に導いて約5℃程度にまで冷却
して冷温スラリーとし、この冷温スラリーをパールミル
、ダイノミル、ゴーリー等の如き破砕装置に導いてその
細胞膜を破砕して破砕スラリーとしこの破砕スラリーを
スプレードライヤーに導いて水分を除去するものである
。なお・高温にするのは調温スラリー中のクロロフィラ
ーゼ酵素を確霊に失活させるためのものであるから、失
活させ得る温度であれば必ずしも上記温度に限定される
ものではない。In order to achieve the above object, the present invention prepares a slurry of chlorophyll-containing single cell colonies such as chlorella in water to a temperature of about 5° C. or lower in a temperature-controlled tank to obtain a low-temperature slurry,
This low-temperature slurry is introduced into an instantaneous heating device to raise the temperature to a high temperature, for example about 110° C., in a very short time, for example within 10 seconds.
The slurry is heated in a trench to about 120°C to form a high-temperature slurry, and this high-temperature slurry is led to a cooling device and cooled to about 5°C to form a cold slurry. The cell membranes are crushed to form a crushed slurry, and this crushed slurry is introduced to a spray dryer to remove water. Note that the purpose of raising the temperature to a high temperature is to completely inactivate the chlorophyllase enzyme in the temperature-controlled slurry, so the temperature is not necessarily limited to the above temperature as long as it can be deactivated.
クロロフィル含有体をゆるやかに加熱するとそのクロロ
フィルにクロロフィラーゼ酵素が作用してこのクロロフ
ィルは毒性7エオフオルバイ・ドに変化させられるもの
である。・本発明においては、このクロロフィラーゼ酵
素を作用温度範囲外の温度に瞬時に昇温せしめると同時
に、その高温により熱破壊を行ない、その活性を失活さ
せる。この失活させることによう、クロロフィルに作用
するいとまを与えずに、破砕処理が行なわれるものであ
る。When a chlorophyll-containing body is gently heated, the chlorophyllase enzyme acts on the chlorophyll, converting the chlorophyll into the toxic 7-eofluoride. - In the present invention, the temperature of this chlorophyllase enzyme is instantaneously raised to a temperature outside the action temperature range, and at the same time, the high temperature is used to thermally destroy the enzyme and deactivate its activity. In order to deactivate the chlorophyll, the crushing treatment is carried out without giving any time to act on the chlorophyll.
破砕処理後の冷却により、わずかに残されたクロロフィ
ラーゼ活性により、クロロフィルが二次的に分解される
のを防ぐ。Cooling after crushing prevents secondary decomposition of chlorophyll due to the small amount of chlorophyllase activity remaining.
実施例について図面を参照して説明する。 Examples will be described with reference to the drawings.
調温タンク1にクロレラの如きクロロフィル含有単細胞
群体が水によってスラリー状にされ、約5℃以下に調温
され、低温スラリーとして収容されている。低温スラリ
ー中のクロレラ等の固形分は、例えば15%とする。A chlorophyll-containing single cell colony such as chlorella is made into a slurry with water, and the temperature is controlled to below about 5° C. and stored as a low-temperature slurry in a temperature-controlled tank 1. The solid content of chlorella or the like in the low-temperature slurry is, for example, 15%.
バルブ13によシ、調温スラリーの出口を開閉する。The valve 13 opens and closes the outlet of the temperature-controlled slurry.
低温スラリーは瞬間加熱装置2に導かれて、極めて短時
間に、例えば約10程度以内に、約 110℃〜120
℃程度の高温にまで加熱されて、高温スラリーとされる
。The low-temperature slurry is led to the instantaneous heating device 2 and heated to about 110°C to 120°C in a very short time, for example within about 10°C.
It is heated to a high temperature of about ℃ to form a high-temperature slurry.
瞬間加熱装置2としては、例えばプレートヒータを用い
ることができる。低温スラリーはモーノポンプ3等によ
って、例えば1〜3 kg/分ぐらいの割合で瞬間加熱
装置2に送シ込まれる。バルブ4、バルブ5によシ処理
流量を調節する。ギヤポンプ又はロータリポンプ等乙に
よって瞬間加熱装置2の中のスラリーは強制循環させら
れ、乱流が起こされ、瞬間的な温度上昇が促進される。As the instant heating device 2, for example, a plate heater can be used. The low-temperature slurry is fed into the instant heating device 2 at a rate of, for example, about 1 to 3 kg/min by a Mono pump 3 or the like. The processing flow rate is adjusted by valves 4 and 5. The slurry in the instantaneous heating device 2 is forced to circulate by a gear pump, rotary pump, etc., causing turbulence and promoting an instantaneous temperature rise.
強制循環は、例えば40〜80リッタ/分とする。The forced circulation is, for example, 40 to 80 liters/minute.
高温スラリーは、バルブ4を通って冷却装置7に導かれ
て約5℃以下にまで冷却されて冷温スラリーとなる。冷
温スラリーはモーノポンプ8によシパールミル、または
ダイノミルの如き破砕装置9に導かれ、細胞膜が破砕さ
れて、破砕スラリーとなる。この破砕スラリーを冷却ク
ツションタンク10に一時ためて約5℃程度のスラリー
とする。このスラリーをプランジャポンプ11にょシス
ブレードライヤー12に導いて約85%の水分を除去し
て破砕粉体、すなわち製品とするものである。The high-temperature slurry is led to a cooling device 7 through a valve 4 and cooled to about 5° C. or lower to become a cold slurry. The cold/hot slurry is guided by a Mono pump 8 to a crushing device 9 such as a Cipal mill or a Dyno mill, where the cell membranes are crushed and a crushed slurry is obtained. This crushed slurry is temporarily stored in a cooling cushion tank 10 to form a slurry at about 5°C. This slurry is introduced into a plunger pump 11 and a system blade dryer 12 to remove about 85% of the moisture to produce a crushed powder, that is, a product.
本実施例によって得られたデータは、およそ次のような
ものである。The data obtained in this example are approximately as follows.
A りCIL/う原料15常濃度の場合1 パールミル
の処理速度1リッタ/分の製品データフェオフォルバイ
ト 20ry%(原料は 20md)クロロフィラー
ゼ活性 0鳩び (原料は800 mg% )2 パ
ールミルの処理速度3リッタ/分の製品データフェオフ
ォルバイト −55mg% (原料は 20 rrd
)クロロフィラーゼ活性1DDmd ←原料は8
D D rrrz% )B クロレラ原料10%濃度の
場合
15%濃度の場合と殆ど同様の結剰;得られた。A RiCIL/raw material 15 Normal concentration 1 Product data of pearl mill processing speed 1 liter/min Pheophorbite 20ry% (raw material is 20 md) Chlorophylase activity 0 Tomobi (raw material is 800 mg%) 2 Pearl mill processing Speed 3 liters/min Product data Pheophorbite -55mg% (Raw material is 20 rrd
) Chlorophylase activity 1DDmd ← Raw materials are 8
D D rrrz% ) B In the case of 10% concentration of chlorella raw material, almost the same residue as in the case of 15% concentration was obtained.
本発明は、以上に説明したような方法であるので、以下
に記載されるような効果を奏する。Since the present invention is a method as described above, it produces effects as described below.
従来の方法の問題点を解決し、消化率が極めて高く、フ
ェオフォルバイトの生成が極めて少なく、厚生省の指導
規準に充分適格であシ、安心して栄養食品として役立て
ることができ、本来のクロレラの持つ健康維持の効果を
高度に発揮することができると共に、更にはクロレラ固
有の鮮明なる緑色をそのまま維持することにより商品価
値を高くすることのできるクロレラ等の細胞膜破砕法が
得られる。It solves the problems of conventional methods, has extremely high digestibility, produces extremely little pheophorbite, fully meets the guidance standards of the Ministry of Health and Welfare, and can be safely used as a nutritional food. A method for disrupting cell membranes of chlorella, etc. can be obtained, which can highly exert the health-maintaining effect of chlorella, and can also increase the commercial value by maintaining the bright green color unique to chlorella.
図は本発明の実施例の系統図である。 図において、に調温タンク 2:瞬間加熱装置 3.8:モーノポンプ 4.5.13:バルブ 6.11:ポンプ 7:冷却装置 9:破砕装置 10=冷却クツシヨンタンク 12ニスプレードライヤー The figure is a system diagram of an embodiment of the present invention. In the figure, the temperature control tank 2: Instant heating device 3.8: Mono pump 4.5.13: Valve 6.11: Pump 7: Cooling device 9: Crushing device 10=Cooling cushion tank 12 Nispray dryer
Claims (1)
よるスラリーを調温タンク中にて約5℃以下に調温して
調温スラリーとし、この調温スラリーを瞬間加熱装置に
導いて極めて短時間に例えば10秒間程度に約110℃
〜120℃位まで加熱して高温スラリーとし、この高温
スラリーを冷却装置に導いて急冷して冷温スラリーとし
、この冷温スラリーをパールミルの如き破砕装置に導い
てその細胞膜を破砕して破砕スラリーとし、この破砕ス
ラリーをスプレードライヤーに導いてその水分を除去す
ることを特徴とするクロレラ等藻類の細胞膜破砕法1. A water slurry of a chlorophyll-containing single cell colony such as chlorella is heated to a temperature of about 5°C or less in a temperature-controlled tank to obtain a temperature-controlled slurry, and this temperature-controlled slurry is introduced into an instantaneous heating device to heat the slurry in a very short period of time, for example, 10°C. Approximately 110℃ for about seconds
The slurry is heated to ~120°C to form a high-temperature slurry, the high-temperature slurry is led to a cooling device and rapidly cooled to form a cold slurry, and the cold slurry is led to a crushing device such as a pearl mill to crush the cell membranes to create a crushed slurry. A method for disrupting cell membranes of algae such as chlorella, which is characterized by introducing this crushed slurry into a spray dryer to remove its water content.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1224295A JPH069490B2 (en) | 1989-08-30 | 1989-08-30 | Cell membrane disruption method of algae containing Chlorella |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP1224295A JPH069490B2 (en) | 1989-08-30 | 1989-08-30 | Cell membrane disruption method of algae containing Chlorella |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH0387164A true JPH0387164A (en) | 1991-04-11 |
JPH069490B2 JPH069490B2 (en) | 1994-02-09 |
Family
ID=16811535
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP1224295A Expired - Lifetime JPH069490B2 (en) | 1989-08-30 | 1989-08-30 | Cell membrane disruption method of algae containing Chlorella |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPH069490B2 (en) |
-
1989
- 1989-08-30 JP JP1224295A patent/JPH069490B2/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH069490B2 (en) | 1994-02-09 |
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