JPH0377823A - Mutation-suppressing agent - Google Patents
Mutation-suppressing agentInfo
- Publication number
- JPH0377823A JPH0377823A JP1212766A JP21276689A JPH0377823A JP H0377823 A JPH0377823 A JP H0377823A JP 1212766 A JP1212766 A JP 1212766A JP 21276689 A JP21276689 A JP 21276689A JP H0377823 A JPH0377823 A JP H0377823A
- Authority
- JP
- Japan
- Prior art keywords
- persimmon
- condensed
- tannin
- mutation
- fruit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000011511 Diospyros Nutrition 0.000 claims abstract description 23
- 244000236655 Diospyros kaki Species 0.000 claims abstract description 23
- 229920001864 tannin Polymers 0.000 claims abstract description 20
- 235000018553 tannin Nutrition 0.000 claims abstract description 20
- 239000001648 tannin Substances 0.000 claims abstract description 20
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 7
- 230000035772 mutation Effects 0.000 claims description 6
- 235000008597 Diospyros kaki Nutrition 0.000 claims description 3
- 239000003112 inhibitor Substances 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 16
- 239000003471 mutagenic agent Substances 0.000 abstract description 13
- 231100000707 mutagenic chemical Toxicity 0.000 abstract description 13
- 230000003505 mutagenic effect Effects 0.000 abstract description 9
- 231100000299 mutagenicity Toxicity 0.000 abstract description 6
- 230000007886 mutagenicity Effects 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 235000011389 fruit/vegetable juice Nutrition 0.000 abstract description 4
- 230000004913 activation Effects 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 abstract description 3
- 230000002503 metabolic effect Effects 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 238000005406 washing Methods 0.000 abstract description 3
- 208000005623 Carcinogenesis Diseases 0.000 abstract description 2
- 239000003463 adsorbent Substances 0.000 abstract description 2
- 230000036952 cancer formation Effects 0.000 abstract description 2
- 231100000504 carcinogenesis Toxicity 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 239000012141 concentrate Substances 0.000 abstract 1
- 235000015203 fruit juice Nutrition 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 239000002592 antimutagenic agent Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- XFOHWECQTFIEIX-UHFFFAOYSA-N 2-nitrofluorene Chemical compound C1=CC=C2C3=CC=C([N+](=O)[O-])C=C3CC2=C1 XFOHWECQTFIEIX-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000723267 Diospyros Species 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- FMMWHPNWAFZXNH-UHFFFAOYSA-N Benz[a]pyrene Chemical compound C1=C2C3=CC=CC=C3C=C(C=C3)C2=C2C3=CC=CC2=C1 FMMWHPNWAFZXNH-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 231100000357 carcinogen Toxicity 0.000 description 2
- 239000003183 carcinogenic agent Substances 0.000 description 2
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 2
- 235000005487 catechin Nutrition 0.000 description 2
- 229920002770 condensed tannin Polymers 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 231100000219 mutagenic Toxicity 0.000 description 2
- 125000005575 polycyclic aromatic hydrocarbon group Chemical group 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- BEFAQJJPFPNXIG-UHFFFAOYSA-N 1-nitro-9h-fluorene Chemical compound C1C2=CC=CC=C2C2=C1C([N+](=O)[O-])=CC=C2 BEFAQJJPFPNXIG-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 229930195730 Aflatoxin Natural products 0.000 description 1
- TXVHTIQJNYSSKO-UHFFFAOYSA-N BeP Natural products C1=CC=C2C3=CC=CC=C3C3=CC=CC4=CC=C1C2=C34 TXVHTIQJNYSSKO-UHFFFAOYSA-N 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- 241001504592 Trachurus trachurus Species 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000005409 aflatoxin Substances 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 150000001765 catechin Chemical class 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000019688 fish Nutrition 0.000 description 1
- 229930182497 flavan-3-ol Natural products 0.000 description 1
- 150000002206 flavan-3-ols Chemical class 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 231100000243 mutagenic effect Toxicity 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 150000002828 nitro derivatives Chemical class 0.000 description 1
- 150000002832 nitroso derivatives Chemical class 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005979 thermal decomposition reaction Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
- Pyrane Compounds (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、渋柿（旧ｏｓｐｙｒｏｓ Ｋａｋｉ Ｔｈｕ
ｒｉｂ）の果実より抽出、精製して得られた檀合型柿タ
ンニンから成る、突然変異抑ｗ１ｗ４に関し、更に詳し
くは、特に２−ニトロフルオレンに代表される直接変異
原に糾する抗変異原性剤に関する。[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to the use of astringent persimmons (formerly Ospyros Kaki Thu).
Concerning mutagenic w1w4, which is composed of tango-type persimmon tannin extracted and purified from the fruit of Rib), more specifically, it has antimutagenicity against direct mutagens, particularly 2-nitrofluorene. Regarding drugs.
我々の日常環境には敗多くの突然変異原性物質が存在し
、これらの多くは発癌物質として、我々の健康を脅かす
原因ともなっている。自然界に存在する変異原では、フ
ラボノイドやフロクマリンなどの植物成分が知られてお
り、また焼き魚中のトリプトファン熱分解物や、代表的
なカビ毒であるアフラトキシン類なども強烈な変異原性
を示す。There are many mutagenic substances in our daily environment, and many of these are carcinogens that threaten our health. Plant components such as flavonoids and furocoumarins are known to be mutagens that exist in nature, and tryptophan thermal decomposition products in grilled fish and aflatoxins, which are typical mycotoxins, also exhibit strong mutagenicity.
また、大気汚染の原因としては、工場排煙や暖房などが
挙げられるが、主たる汚染源である自動車排ガスは特に
部会における重大な社会問題の一つである。この自勅車
排ガス中には各種のニトロ化合物や多環芳香族炭化水素
が含まれており、強い変異原性を有する化合物が多い。In addition, while the causes of air pollution include factory smoke and heating, automobile exhaust gas, which is the main source of pollution, is one of the most important social issues for the committee. The exhaust gas from privately-owned vehicles contains various nitro compounds and polycyclic aromatic hydrocarbons, many of which have strong mutagenic properties.
、−トロピレン。, -tropylene.
ベンゾ（ａ）ピレン、ニトロフルオレンなどであるが、
とりわけ、２−ニトロフルオレンは生体内に於ける代謝
活性化を必要としない強い直接変異原であり、また発癌
物質でもある。benzo(a)pyrene, nitrofluorene, etc.
In particular, 2-nitrofluorene is a strong direct mutagen that does not require metabolic activation in vivo, and is also a carcinogen.
これらの変異原に対し、その化学的構造の解明だけでな
く、生体に及ぼず作用機構を明らかにし、さらには生体
に作用するのを防止するための努力も続りられている８
例えば、野菜などに含まれる食物繊鯵は変異原を１薯す
る作用があるとされ、またビタミンＣも還元作用により
ニトロソ化合物の生成を抑制することがわかり、変異原
の不活性化機構も次第に明らかになってきた。そのなか
に於て、強い変異原性を有する多環芳香族炭化水木のう
ぢ、２−ニトロフルオレンのように直接的に変異原性を
示す直接変異原に対しては今のところ有効な除去物質は
見出されていない。Efforts are being made not only to elucidate the chemical structure of these mutagens, but also to elucidate their mechanism of action without affecting living organisms, and further efforts to prevent them from acting on living organisms8.
For example, the dietary fiber contained in vegetables, horse mackerel, is said to have the effect of reducing mutagens, and vitamin C has also been found to suppress the production of nitroso compounds through its reducing action, and the mechanism for inactivating mutagens is gradually becoming more active. It's becoming clear. Among these, there are currently no effective removal methods against direct mutagens such as polycyclic aromatic hydrocarbons, which have strong mutagenicity, and 2-nitrofluorene. No substance has been found.
（発明が解決しようとする課題）
すなわち、本発明の目的は、２−二トロフルオレンに代
表される直接変異原に対する抗変異原性剤を従供するこ
とにある。(Problems to be Solved by the Invention) That is, an object of the present invention is to provide an antimutagenic agent against a direct mutagen represented by 2-nitrofluorene.
（課題を解決するための手段〕
本発明は、渋柿（Ｄｉｏｓｐｙｒｏａ Ｋａｋｉ Ｔｈ
ｕｎｂ）の果実より抽出、精製して得られた縮合型柿タ
ンニンから成る、突然変異抑制剤である。(Means for Solving the Problems) The present invention is directed to the cultivation of astringent persimmons (Diospyroa Kaki Th.
This is a mutation suppressor consisting of condensed persimmon tannin extracted and purified from the fruits of M. unb).
タンニンは加水分解型タンニンと縮合型タンニンに分け
られ、前者は糖または環状多価アルコールにガロイル基
またはその誘導体が結合したもので、後者はカテキン、
エビカテキンのようなフラバン−３−オールなどの縮合
体である。Tannins can be divided into hydrolyzed tannins and condensed tannins, the former consisting of sugars or cyclic polyhydric alcohols bound to galloyl groups or their derivatives, and the latter consisting of catechins,
It is a condensate of flavan-3-ols such as shrimp catechin.
本発明における縮合型棒タンニンは下記の構造式（１）
のごとく推定されている（松属、伊藤、化学と生物、
Ｖｏｌ、１５．４１１．７３２−６（１９７７））。The condensed tannin in the present invention has the following structural formula (1):
It is estimated that (pine genus, Ito, chemistry and biology,
Vol. 15.411.732-6 (1977)).
尚、松属らによれば重量平均分子量は１．３８ ｘ１０
４、数平均分子量は０．６４Ｘ１０’ と言われ縮合型
柿タンニンの製造法としては、例えば下記の方法が例示
される。Furthermore, according to Matsugen et al., the weight average molecular weight is 1.38 x 10
4. The number average molecular weight is said to be 0.64 x 10', and the following method is exemplified as a method for producing condensed persimmon tannin.
渋柿（Ｄｉｏｓｐｙｒｏｓ Ｋａｋｌ Ｔｈｕｎｂ）の
果実を小細片とし、圧搾して果汁を採取した後、−過に
より固形分を除去して柿渋を得る。この柿渋にエタノー
ルを添加するかまたは添加せずして、これを、合成吸着
ｊ１１（例えばダイヤイオンＩ Ｐ ２０ 、三菱化成
工業■製）を充填したカラムに接触吸着させ、そのカラ
ムを水洗した後、含水エタノールを用いて溶出させる。The fruit of Diospyros Kakl Thunb is cut into small pieces, the juice is collected by squeezing, and the solid content is removed by filtration to obtain persimmon astringent. This persimmon tannin, with or without the addition of ethanol, is catalytically adsorbed in a column filled with synthetic adsorption J11 (for example, Diaion I P 20, manufactured by Mitsubishi Chemical Industries, Ltd.), and after washing the column with water. , and elute using aqueous ethanol.
この溶出液を減圧下で濃縮した後、凍結乾燥して縮合型
柿タンニンを得る。This eluate is concentrated under reduced pressure and then freeze-dried to obtain condensed persimmon tannin.
また、別の方法としては、溶媒沈澱法（松属伊藤、「化
学と生物Ｊ 、 Ｖｏｌ、１５．１ｔｉｌ １．７３３
（１９７７） ）や、特開昭５６−５１４９７号公報に
記載の方法等が挙げられる。Another method is the solvent precipitation method (Matsugen Ito, Chemistry and Biology J, Vol. 15.1til 1.733).
(1977)) and the method described in Japanese Patent Application Laid-Open No. 56-51497.
本発明に使用する縮合型柿タンニンは、水、エタノール
、メタノールに易溶で、ピリジンに可溶であり、アセト
ン、エーテル−、クロロホルム。The condensed persimmon tannin used in the present invention is easily soluble in water, ethanol, and methanol, and soluble in pyridine, acetone, ether, and chloroform.
石油ベンジン、酢酸に難溶である。Slightly soluble in petroleum benzene and acetic acid.
ｍ＝２〜５
ング手法として近年確立されたエームズ法を用いて試験
した所、優れた突然変異抑制活性を示し、特に代謝活性
化を必要としない直接変異原による変異原性を阻害する
発癌予防上重要な意義を有する。m = 2 to 5 When tested using the Ames method, which has been established in recent years, it showed excellent mutation-suppressing activity, and is particularly effective in preventing carcinogenesis by inhibiting mutagenicity caused by direct mutagens that do not require metabolic activation. It has important significance.
（１）縮合型棒タンニンの製造法
渋柿（口１ｏａｐｙｒｏｓ Ｋａｋｉ Ｔｈｕｎｈ）の
果実を小絹片とし、圧搾して果汁を採取した後、浜過に
より固形分を除去して柿渋を得た。柿渋に適量のエタノ
ールを添加し、このエタノールを含有した柿渋１０００
ｋｇを、合成吸着剤のダイヤイオンＨＦ２０（三菱化成
工業■製）１００１を充填したカラムに接触吸着させ、
このカラムをＩｌｌ製水２０００１で水洗した後、含水
エタノール２０００Ｉ！を用いて溶出させた６次にこの
溶出液を減圧下で濃縮した後、凍結乾燥して、縮合型柿
タンニン３０ｋｇを得たゆ
（２）突然変異抑制効果の測定方法
（ｉ）突然変異原性試験方法
突然変異原性の測定には、発癌性のスクリー、ニング手
法として近年確立されたエームズ法を用い、菌株はサル
モネラ・ティフィムリウムＴＡ９８株（以ＰＴＡ９８と
略す）を使用した。(1) Method for producing condensed rod tannin The fruit of astringent persimmon (Kaki Thunh) was cut into small pieces of silk, and the juice was collected by squeezing, and the solid content was removed by filtering to obtain persimmon astringency. Kakishibu 1000, which contains ethanol, is made by adding an appropriate amount of ethanol to persimmon juice.
kg was catalytically adsorbed in a column packed with a synthetic adsorbent, Diaion HF20 (manufactured by Mitsubishi Chemical Industries, Ltd.) 1001.
After washing this column with Ill Water 20001, aqueous ethanol 2000I! Next, this eluate was concentrated under reduced pressure and lyophilized to obtain 30 kg of condensed persimmon tannin. (2) Method for measuring mutation suppression effect (i) Mutagen Sex test method: To measure mutagenicity, the Ames method, which has been established in recent years as a screening method for carcinogenicity, was used, and Salmonella typhimurium strain TA98 (hereinafter abbreviated as PTA98) was used.
（ｉｉ ）測定試料の調製
柿タンニン（以下抗変異原と略す）を水に１０ｒｎｇ／
ｍ１の濃度で溶解し、６段階に希釈し、た。(ii) Preparation of measurement sample 10rng/persimmon tannin (hereinafter abbreviated as antimutagen) in water.
It was dissolved at a concentration of ml and diluted in 6 steps.
調製した。Prepared.
（ｉｉ ）突然変異原性の測定
抗変異原の各濃度溶液１００μｆに対し、１００ｍＭリ
ン酸すトリウム緩衝１（ｐＨ７，４）５００μ！及びＴ
ａ２Ｏの前培養液１００μｌを混合し、これに１−ＮＦ
２０μ戸を加え撹はんしたのち、２ｍ７！の軟寒天を加
えて、鹸小グルコース寒天平板墳地１に重層固化し、３
７℃にて２日間培養した。培養後、プレー１〜上の復帰
変異コロニー数をカウントした。(ii) Measurement of mutagenicity 500μf of 100mM sodium phosphate buffer 1 (pH 7,4) per 100μf of each concentration solution of antimutagen! and T
Mix 100 μl of a2O preculture solution and add 1-NF to this.
After adding 20μ and stirring, 2m7! of soft agar was added to the solidified small glucose agar flat plate mound 1, and solidified in layers.
The cells were cultured at 7°C for 2 days. After culturing, the number of revertant colonies on plates 1 to 1 was counted.
抗変異原性の評価は、変異原および抗変異原を加えた系
でカウントしたプレート当りのコロニー数（Ａ）、変異
原のみのコロニー数（Ｂ）、水のみの自然復帰コロニー
数（Ｃ）をもとに次式により突然変異率を算出して行な
った。Antimutagenicity was evaluated using the number of colonies per plate counted in the system containing mutagen and antimutagen (A), the number of colonies with mutagen only (B), and the number of naturally reverted colonies with water only (C). Based on this, the mutation rate was calculated using the following formula.
（２）結果
（縮合型棒タンニンの突然変異抑制活性）表１に示した
通り、縮合型柿タンニン無添加の場合を１００％とした
時、抗変異原の濃度上昇とともに突然変異率の減少が明
らかに認められた。(2) Results (mutation suppression activity of condensed rod tannins) As shown in Table 1, when the case without condensed persimmon tannins is taken as 100%, the mutation rate decreases as the concentration of antimutagen increases. clearly recognized.
従って縮合型棒タンニンの２−ＮＦに対する突然表
１、 ２
ＮＦに対する縮合型棒タンニンの効果
０ ５ １３
６７ ５ １１
３３ ５ １０００
５ ９６７ ５
９
３３ ５ ８
００ ５ ７０
＊：ＨおＯのみのコロニー数Therefore, the effect of condensed rod tannins on 2-NF suddenly Table 1, 2 Effect of condensed rod tannins on NF 0 5 13 67 5 11 33 5 1000
5 967 5
9 33 5 8 00 5 70 *: Number of colonies of H and O only
Claims (1)
果実より抽出、精製して得られた縮合型柿タンニンから
成る、突然変異抑制剤。A mutation inhibitor consisting of condensed persimmon tannin extracted and purified from the fruit of Diospyros Kaki Thunb.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1212766A JPH0377823A (en) | 1989-08-17 | 1989-08-17 | Mutation-suppressing agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1212766A JPH0377823A (en) | 1989-08-17 | 1989-08-17 | Mutation-suppressing agent |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0377823A true JPH0377823A (en) | 1991-04-03 |
Family
ID=16628044
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1212766A Pending JPH0377823A (en) | 1989-08-17 | 1989-08-17 | Mutation-suppressing agent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0377823A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04190774A (en) * | 1990-11-26 | 1992-07-09 | Kikkoman Corp | Antimutagenic agent |
| JP2001336544A (en) * | 2000-03-22 | 2001-12-07 | Aisin Seiki Co Ltd | Friction clutch for vehicle |
| US20100240600A1 (en) * | 2007-06-12 | 2010-09-23 | Hiroshima University | Anti-Norovirus Agent and Composition Containing the Same |
-
1989
- 1989-08-17 JP JP1212766A patent/JPH0377823A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04190774A (en) * | 1990-11-26 | 1992-07-09 | Kikkoman Corp | Antimutagenic agent |
| JP2857646B2 (en) * | 1990-11-26 | 1999-02-17 | キッコーマン株式会社 | Anti-mutagenic agent |
| JP2001336544A (en) * | 2000-03-22 | 2001-12-07 | Aisin Seiki Co Ltd | Friction clutch for vehicle |
| US20100240600A1 (en) * | 2007-06-12 | 2010-09-23 | Hiroshima University | Anti-Norovirus Agent and Composition Containing the Same |
| US8431168B2 (en) * | 2007-06-12 | 2013-04-30 | Hiroshima University | Anti-norovirus agent and composition containing the same |
| US8790718B2 (en) | 2007-06-12 | 2014-07-29 | Hiroshima University | Method of disinfection or infection control against norovirus |
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