JPH0367650B2 - - Google Patents

Description

[Detailed description of the invention]

The present invention relates to a method for producing horseradish seeds, and more particularly, to a method for producing horseradish seeds that can produce high-quality horseradish seeds in large quantities. Horseradish is widely used as the main raw material for powdered wasabi, etc., but because seeds cannot be produced during cultivation, the current practice is to rely solely on vegetative propagation. The so-called seed roots currently used for propagation are made by selecting particularly large horseradish from the harvested horseradish and cutting out the lateral roots, as shown in Reference Photo 1. At this time, if the lateral roots become thin or short, the next year's yield will be greatly reduced, so it takes a lot of effort and nerves to collect the roots every year. However, it is still extremely difficult to obtain high-quality seeds every year. In addition, since this cultivation relies only on vegetative propagation, viruses and various pathogens are completely transmitted from the parent plant, and on top of that, only 5 to 10 seed roots can be collected from each plant, which is an inefficient method of seed collection. Must be. The purpose of the present invention is to eliminate viruses caused by vegetative propagation.
It is an object of the present invention to provide a method for producing horseradish seeds of high quality in large quantities and easily, which improves the drawbacks of the above-mentioned known techniques, such as morbidity caused by various pathogenic bacteria and inefficient seed collection. In order to achieve the aforementioned object, according to the present invention:
It is characterized by consisting of the following three steps (a), (b) and (c). (b) A step of placing horseradish tissue on a sterile medium and culturing it to directly produce seedlings from the tissue without going through callus. (b) A step of growing the young plant into a seedling. (c) A step of growing underground stems of the seedlings to produce seed roots. Hereinafter, the present invention will be specifically explained in detail. (b) The process of placing horseradish tissue on a sterile medium and culturing it to grow seedlings. Young plants are grown directly from tissues near the growing point of horseradish. First, prepare Murashige E Skoog basic medium with the following composition.

[Table] Dispense the above-mentioned basic medium into culture tubes and sterilize it under high pressure in an autoclave to prepare a sterile medium. The sterile medium used in the present invention may be the above-mentioned basic medium to which growth regulators, cytokinins, oxins, etc. are added, or agar may be added to this to form a solid medium, or agar may be added to this to form a solid medium. A liquid medium may be used instead. Furthermore, after disinfecting the tissue near the growing point of horseradish (the part including the growing point part) with a 0.5% sodium hypochlorite solution, the growing point tissue was aseptically extracted from this material and placed on the sterile medium. , to culture. When the culture medium is a liquid medium, the tissue is placed aseptically, for example, on a filter paper, and the liquid medium is absorbed into the filter paper for culturing. The culturing is carried out at a temperature of 15 to 25° C. by irradiating with light at an intensity of 3000 lux for 12 to 16 hours a day. After 2 to 3 months, it forms leaves, roots, and grows into a young plant with a height of 1 to 5 cm. Reference photo 2 is a photo showing the growth state of horseradish growing point tissue placed on the above-mentioned sterile medium after one month of culture. (b) A step of growing the young plant into a seedling. The seedling obtained in step (a) above is taken out of the culture tube, planted, for example, in a pot containing heat-sterilized vermiyquilite, and kept in a greenhouse. The pot in which the seedling is planted is covered with a glass beaker, a vinyl sheet, etc. to keep the humidity as high as possible, and mist cultivation is performed at a temperature of 15 to 25°C to allow the roots to take root. Once the roots have taken root, remove the cover and grow into seedlings (live weight 10-50g).
Reference photo 3 is a photo showing this growth state.
It is also possible to grow the seedlings into seedlings under sterile conditions by placing the seedlings in a large Erlenmeyer flask or container containing a sterile medium and providing natural light or illuminance close to natural light. (See reference photo 4.) In addition, the process (b) of growing the young plants into seedlings mentioned above
may be carried out consecutively with step (a) using the sterile medium shown in (a) above. (c) A step of growing the seedlings or underground stems of the seedlings to produce seed roots. The seedlings grown in step (b) above or the rhizomes from which the leaves and roots of these seedlings have been removed are planted in a seed field in early spring (around April to May), and then planted in late autumn (November to December).
Horseradish seeds are grown until around 300 days (about 30 days) to obtain horseradish seed roots for seed collection. (See reference photo 5). Comparative tests were conducted on the number of seed roots (per plant) and yield of the seed roots according to the present invention produced in this way and conventional ones, and the results are shown in Tables 1 and 2.

【table】

[Table] As shown in Table 1, in the conventional seed collection method, only lateral roots that can be used as seed roots (0.5 to 2 cm in diameter,
(20 to 25 cm in length), it was possible to collect only 5 to 10 seeds per plant (see reference photo 1), but with the method of the present invention, about 50 to 90 seeds could be collected. (Reference photo 5
) Also, the following year, this seed root was planted in a general field,
We compared the yield with conventional seed roots. As a result, as is clear from Table 2, when the seed roots collected by the present invention are used, the yield is more than double compared to the seed roots collected by the conventional method, and various diseases do not develop. It was confirmed that the seeds were of high quality. As described above, it can be seen that high quality horseradish seeds can be produced in large quantities according to the method of the present invention.

Claims (1)

[Scope of Claims] 1. A seed root production method capable of producing a large amount of high quality horseradish seed roots, which comprises the following three steps (a), (b), and (c). (a) A step of placing tissue near the growth point of horseradish on a sterile medium, culturing it, and growing seedlings directly from the tissue without passing through callus. (b) A step of growing the young plant into a seedling. (c) A step of growing underground stems of the seedlings to produce seed roots.