JPH0346564A - Immunoassay and immune sensor - Google Patents
Immunoassay and immune sensorInfo
- Publication number
- JPH0346564A JPH0346564A JP18291889A JP18291889A JPH0346564A JP H0346564 A JPH0346564 A JP H0346564A JP 18291889 A JP18291889 A JP 18291889A JP 18291889 A JP18291889 A JP 18291889A JP H0346564 A JPH0346564 A JP H0346564A
- Authority
- JP
- Japan
- Prior art keywords
- detected
- reaction phase
- substance
- immunoassay method
- optical fiber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003018 immunoassay Methods 0.000 title claims description 19
- 238000006243 chemical reaction Methods 0.000 claims abstract description 38
- 239000013307 optical fiber Substances 0.000 claims abstract description 24
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 20
- 239000000126 substance Substances 0.000 claims description 20
- 229960001252 methamphetamine Drugs 0.000 claims description 17
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 claims description 15
- 239000000015 trinitrotoluene Substances 0.000 claims description 12
- 239000007850 fluorescent dye Substances 0.000 claims description 11
- SPSSULHKWOKEEL-UHFFFAOYSA-N 2,4,6-trinitrotoluene Chemical compound CC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O SPSSULHKWOKEEL-UHFFFAOYSA-N 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 9
- 125000001295 dansyl group Chemical group [H]C1=C([H])C(N(C([H])([H])[H])C([H])([H])[H])=C2C([H])=C([H])C([H])=C(C2=C1[H])S(*)(=O)=O 0.000 claims description 7
- 239000012491 analyte Substances 0.000 claims description 6
- 230000007423 decrease Effects 0.000 claims description 6
- 230000005284 excitation Effects 0.000 claims description 6
- 239000013076 target substance Substances 0.000 claims description 6
- KWTSXDURSIMDCE-QMMMGPOBSA-N (S)-amphetamine Chemical compound C[C@H](N)CC1=CC=CC=C1 KWTSXDURSIMDCE-QMMMGPOBSA-N 0.000 claims description 2
- 229940025084 amphetamine Drugs 0.000 claims description 2
- KWGRBVOPPLSCSI-WPRPVWTQSA-N (-)-ephedrine Chemical compound CN[C@@H](C)[C@H](O)C1=CC=CC=C1 KWGRBVOPPLSCSI-WPRPVWTQSA-N 0.000 claims 2
- 239000007853 buffer solution Substances 0.000 claims 1
- 230000002860 competitive effect Effects 0.000 claims 1
- KWGRBVOPPLSCSI-UHFFFAOYSA-N d-ephedrine Natural products CNC(C)C(O)C1=CC=CC=C1 KWGRBVOPPLSCSI-UHFFFAOYSA-N 0.000 claims 1
- 229960002179 ephedrine Drugs 0.000 claims 1
- 238000007654 immersion Methods 0.000 claims 1
- 239000000463 material Substances 0.000 abstract 6
- 230000005540 biological transmission Effects 0.000 abstract 2
- 230000004936 stimulating effect Effects 0.000 abstract 2
- 230000021615 conjugation Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 4
- 230000000171 quenching effect Effects 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Abstract
Description
【発明の詳細な説明】
産業上の利用分野
本発明（よ 主として臨床検査における病原体あるいは
疾病マーカーなどの検蟲 さらには広〈産業上の極微量
の物質を簡便にかつ迅速に測定する免疫測定方法および
免疫センサーに関する。[Detailed Description of the Invention] Industrial Fields of Application The present invention (mainly used in clinical tests to detect pathogens or disease markers, etc.) and also widely used in industrial applications (immunoassay method for simply and quickly measuring extremely small amounts of substances) and regarding immune sensors.
従来の技術
従来 極微量の物質の迅速かつ高感度な免疫的測定法に
（よ 被検出物質あるいは蛍光プローブで標識された被
検出物質（蛍光プローブ標識被検出物質）の結合による
抗体および蛍光プローブの蛍光強度の変化を蛍光測定装
置内で測定する方法が用いられて来た この方法は あ
らかじめ蛍光測定装置内のミクロセルに抗体溶液、また
は蛍光プローブを用いる場合は抗体溶液と蛍光プローブ
標識被検出物質を注入しておき、次に被検出物質を加え
て抗体に結合させ、一定時間経過後の蛍光強度を測定す
ることによって被検出物質の量を測定するものである。Conventional technology Conventional Rapid and highly sensitive immunoassay methods for ultra-trace amounts of substances include the use of antibodies and fluorescent probes by binding to the analyte or the analyte labeled with a fluorescent probe (fluorescent probe-labeled analyte). A method has been used in which changes in fluorescence intensity are measured within a fluorometer. In this method, an antibody solution, or when using a fluorescent probe, an antibody solution and a fluorescent probe-labeled analyte is placed in a microcell in the fluorometer in advance. The amount of the detected substance is measured by adding the substance to be detected and binding it to the antibody, and measuring the fluorescence intensity after a certain period of time.
発明が解決しようとする課題
前項で記載したように従来の免疫的検出方法では被検出
溶液を蛍光測定装置内のミクロセルに注入し 一定時間
後に蛍光強度を測定しなければならず、被検出溶液の運
搬が困難なものや被検出溶液の数が多い時にはミクロセ
ルへの被検出溶液の注入に時間と手間がかかり、これを
改善する方法が望まれていた
本発明は このような従来技術の課題を解決することを
目的とすも
課題を解決するための手段
本発明は 従来蛍光測定装置内に備えられていた反応相
を蛍光測定装置外に出し 光ファイバーで蛍光測定装置
と反応相を結び励起光と出力光を伝達する方法によって
被検出物質の検出を行なう。Problems to be Solved by the Invention As described in the previous section, in the conventional immunodetection method, the solution to be detected must be injected into a microcell in a fluorometer and the fluorescence intensity must be measured after a certain period of time. When transporting something difficult or when there are a large number of solutions to be detected, it takes time and effort to inject the solutions to be detected into the microcell, and a method to improve this has been desired.The present invention solves these problems of the conventional technology. The purpose of the present invention is to take the reaction phase, which was conventionally provided inside the fluorescence measurement device, out of the fluorescence measurement device, and to connect the fluorescence measurement device and the reaction phase with an optical fiber to connect the excitation light and the reaction phase. The substance to be detected is detected by a method of transmitting output light.
この反応相は検出物質は透過するが抗体は透過しない半
透膜に覆われており、反応相を検出溶液に直接浸すと、
被検出溶液中の被検出物質が半透膜を通過して反応相に
入り、これが反応相内の抗体と結合する事により蛍光強
度が変化して被検出溶液中の被検出物質の濃度が測定さ
れる。This reaction phase is covered with a semipermeable membrane that allows the detection substance to pass through but not the antibody, and when the reaction phase is directly immersed in the detection solution,
The target substance in the target solution passes through the semi-permeable membrane and enters the reaction phase, and when this binds with the antibody in the reaction phase, the fluorescence intensity changes and the concentration of the target substance in the target solution is measured. be done.
作用
本発明ｔ−１上記の手段により、被検出溶液の運搬が困
難なものや被検出溶液の数が多いものでも簡便に極微量
の物質の迅速かつ高感度な測定が可能となる。Effects of the present invention t-1 By means of the above-mentioned means, it is possible to easily and quickly and highly sensitively measure an extremely small amount of a substance even when it is difficult to transport a detection solution or when there are a large number of detection solutions.
実施例
以下に 本発明の実施例について図面を参照しながら説
明する。EXAMPLES Below, examples of the present invention will be described with reference to the drawings.
実施例１
トリニトロトルエンの免疫的測定方法；抗トリニトロト
ルエン抗体は２８０ ｎｍの励起光による３４０ｎｍの
蛍光がトリニトロトルエンと結合することにより減少す
る性質（蛍光消光性）を持１ この抗体を光ファイバー
の先端に備えられた反応相に加入 反応相を直接被検出
溶液に浸し 蛍光の伝達を光ファイバーで行うことによ
って被検出物質を検出した
（Ａ）バッファー（ｐＨ７のリン酸バッファーを０．４
５μｍのフィルターでろ過したもの）で抗トリニトロト
ルエン抗体を溶解して２Ｘ１０−’Ｍの濃度としｆＱ、
この溶液３８０μｌを反応相（２０℃に設定）に加
え１． この反応相に２８０ｎｍの蛍光を光ファイバ
ーにより伝達し３４０ ｎｍの蛍光を光ファイバーによ
り取り出して強度を測定すると蛍光強度は約４５であっ
１゜
（Ｂ）上記（Ａ）のセンサーの反応相を種々の濃度のト
リニトロトルエン溶液に浸し２８０ｎｍ励起の３４０
ｎｍの蛍光強度を測定するとトリニトロトルエンの濃度
に依存して３４０ ｎｍの蛍光強度は減少しｔも 減
少率■ （％）を、次式を用いて算出した
ここで、Ｆはトリニトロトルエン溶液に反応相を浸す前
の蛍光強度 またＦ ｏｂｓは反応相をトリニトロトル
エン溶液に浸したときの蛍光強度を示す。Example 1 Immunoassay method for trinitrotoluene; anti-trinitrotoluene antibody has a property (fluorescence quenching property) that the fluorescence at 340 nm caused by excitation light at 280 nm decreases when combined with trinitrotoluene.1 This antibody was used at the tip of an optical fiber. The reaction phase was directly immersed in the target solution, and the target substance was detected by transmitting fluorescence through an optical fiber.
Dissolve the anti-trinitrotoluene antibody to a concentration of 2 x 10-'M with fQ,
Add 380 μl of this solution to the reaction phase (set at 20°C) 1. Fluorescence at 280 nm was transmitted to this reaction phase through an optical fiber, and fluorescence at 340 nm was extracted through an optical fiber and the intensity was measured.The fluorescence intensity was approximately 45 degrees. 340 nm excitation at 280 nm.
When measuring the fluorescence intensity at 340 nm, the fluorescence intensity at 340 nm decreased depending on the concentration of trinitrotoluene, and the decrease rate (%) was calculated using the following formula. Fluorescence intensity before immersing the phase F obs indicates the fluorescence intensity when the reaction phase is immersed in the trinitrotoluene solution.
トリニトロトルエンの濃度に対する蛍光強度の減少率を
第１図に示す。第１図の結果から、約１０−７６Ｍの濃
度のトリニトロトルエンが本発明により検出できたこと
が証明され丸
な抵 本実施例では被検出物質と結合時に蛍光強度が消
光する性質を持つ抗体として抗トリニトロトルエン抗体
を用いた力丈 一般にこのような蛍光消光についての性
質を持つ抗体を用いれば上記の方法での極微量の物質の
検出が可能である。FIG. 1 shows the rate of decrease in fluorescence intensity with respect to the concentration of trinitrotoluene. The results shown in Figure 1 prove that trinitrotoluene at a concentration of about 10-76M can be detected by the present invention. Powerfulness using anti-trinitrotoluene antibody Generally, it is possible to detect trace amounts of substances using the above method by using antibodies that have such fluorescence quenching properties.
実施例２
メタンフェタミンの免疫的測定方法；
抗メタンフエタミン抗体は蛍光消光性は持っていない力
丈 ダンシル基で標識したメタンフェタミン（ダンシル
標識メタンフェタミン）と結合した時、 ２８０ｎｍの
波長で励起すると５３０ ｎｍの波長の蛍光強度が増強
する性質を持１ この抗体とダンシル標識メタンフェタ
ミンを光ファイバーの先端に備えられた反応相に角丸
反応相を直接被検出溶液に浸し 蛍光の伝達を光ファイ
バーで行うことによって被検出物質を検出し１゜（Ａ）
バッファー（ｐＨ７のリン酸バッファーを０．４５μｍ
のフィルターでろ過したもの）で抗メタンフエタミン抗
体を溶解して２Ｘ１０−’Ｍの濃度とした この溶液３
６０μｍを反応相（２０℃に設定）に入れ さらに１０
”’Ｍのダンシル標識メタンフェタミンを２０μｌ加え
１． この反応相に２８０ ｎｍの蛍光を光ファイバ
ーにより伝達し５３０ｎｍの蛍光を光ファイバーにより
取り出してその強度を測定すると蛍光強度は約６０であ
った
（Ｂ）上記（Ａ）のセンサーの反応相を種々の濃度のメ
タンフェタミン溶液に浸し２８０ ｎｍ励起の５３０ｎ
ｍの蛍光強度を測定するとメタンフェタミンの濃度に依
存して５３０ｎｍの蛍光強度は減少した 減少率■ （
％）を、次式を用いて算出しｔも
ここで、Ｆは反応相をメタンフェタミン溶液に浸す前の
蛍光強度、またＦ ｏｂｓは反応相をメタンフェタミン
溶液に浸したときの蛍光強度を示す。メタンフェタミン
の濃度に対する蛍光強度の減少率を第２図に示す。第２
図の結果から約１０−ＬＳＭの濃度のメタンフェタミン
が本発明により検出できたことが証明された
また ダンシル基で標識したメタンフェタミンの代わり
にダンシル基で標識したアンフェタミンを用いても同様
の結果を得ることができた以上 主としてメタンフェタ
ミンの検出を例にＱ−
とって本発明の説明を行なった力交 もちろんその他の
化学物質すべてに応用可能な一般的方法である。Example 2 Immunoassay method for methamphetamine; Anti-methamphetamine antibodies do not have fluorescence quenching properties. When combined with methamphetamine labeled with a dansyl group (dansyl-labeled methamphetamine), when excited at a wavelength of 280 nm, it emits a light of 530 nm. This antibody and dansyl-labeled methamphetamine have the property of increasing the fluorescence intensity at different wavelengths.
The reaction phase is directly immersed in the target solution, and the target substance is detected by transmitting fluorescence using an optical fiber.
Buffer (0.45 μm pH 7 phosphate buffer)
The anti-methamphetamine antibody was dissolved in this solution 3 to a concentration of 2 x 10-'M (filtered through a filter).
60 μm into the reaction phase (set at 20 °C) and an additional 10
1. Add 20 μl of dansyl-labeled methamphetamine of M. 1. Transmit 280 nm fluorescence to this reaction phase using an optical fiber, extract 530 nm fluorescence using an optical fiber, and measure its intensity. The fluorescence intensity was approximately 60 (B) above. The reaction phase of the sensor in (A) was immersed in methamphetamine solutions of various concentrations and 530n of 280nm excitation was applied.
When measuring the fluorescence intensity of m, the fluorescence intensity of 530 nm decreased depending on the concentration of methamphetamine. Decrease rate ■ (
%) is calculated using the following formula, where t is also the fluorescence intensity before the reaction phase is immersed in the methamphetamine solution, and F obs is the fluorescence intensity when the reaction phase is immersed in the methamphetamine solution. FIG. 2 shows the rate of decrease in fluorescence intensity with respect to the concentration of methamphetamine. Second
The results shown in the figure prove that methamphetamine at a concentration of about 10-LSM can be detected by the present invention.Also, similar results can be obtained by using amphetamine labeled with a dansyl group instead of methamphetamine labeled with a dansyl group. Now that the present invention has been explained, mainly taking the detection of methamphetamine as an example, it is of course a general method that can be applied to all other chemical substances.
実施例３
免疫センサー；
実施例１および２で説明した反応相を光ファイバーを介
して蛍光測定装置に結合させ、免疫センサーを作製した
第３図（ａ）に示すように 測定機構（よ 蛍光測定
装置ｌで調整した波長の光を光ファイバー２によって反
応相３に伝達し 反応相３内の抗体を励起し 反応相３
より出力された蛍光を光ファイバー２によって蛍光測定
装置ｌに伝達しここで蛍光強度を測定するものである。Example 3 Immunosensor: An immunosensor was fabricated by coupling the reaction phase described in Examples 1 and 2 to a fluorescence measuring device via an optical fiber.As shown in FIG. The light with the wavelength adjusted by l is transmitted to the reaction phase 3 through the optical fiber 2 to excite the antibodies in the reaction phase 3.
The fluorescence output from the fluorescent light source is transmitted to a fluorescence measuring device 1 through an optical fiber 2, and the fluorescence intensity is measured there.
第３図（ｂ）４ｉ その反応相３を中心とする暗示断
面図である。半透膜５がＯリング４を介して、光ファイ
バー２の先端に取り付けられている。FIG. 3(b) 4i is a implied cross-sectional view centered on the reaction phase 3. A semipermeable membrane 5 is attached to the tip of the optical fiber 2 via an O-ring 4.
な抵 実施例１および実施例２は本免疫センサーを用い
て行なったものである。Examples 1 and 2 were conducted using the present immunosensor.
また 光ファイバー２の末端に備えられた反応相３が光
ファイバー２と脱着が可能であってもよＩＯ−
発明の効果
本発明により、水溶液中の極微量物質を迅速にかつ簡便
に測定することが可能になったFurthermore, the reaction phase 3 provided at the end of the optical fiber 2 may be detachable from the optical fiber 2. Effects of the Invention According to the present invention, it is possible to quickly and easily measure trace amounts of substances in an aqueous solution. Became
第１図（ま本発明の実施例１の免疫的測定方法によって
測定した各トリニトロトルエン濃度における蛍光減少率
を示したグラフ、第２図は本発明の実施例２の免疫的測
定方法によって測定した各メタンフェタミン濃度におけ
る蛍光減少率を示したグラフ、第３図は本発明における
免疫センサーの暗示断面図であり、 （ａ）は免疫セン
サーの全体暗示は （ｂ）は反応相付近の拡大図を示す
ものである。
１・・・蛍光測定装置 ２・・・光ファイバー、３・・
・反応祖 ４・・・Ｏリング、 ５・・・半透風Figure 1 (a graph showing the fluorescence reduction rate at each trinitrotoluene concentration measured by the immunoassay method of Example 1 of the present invention), and Figure 2 is a graph showing the fluorescence reduction rate at each trinitrotoluene concentration measured by the immunoassay method of Example 2 of the present invention. A graph showing the fluorescence reduction rate at each methamphetamine concentration, and FIG. 3 is a cross-sectional view of the immunosensor according to the present invention. 1... Fluorescence measurement device 2... Optical fiber, 3...
・Reaction starter 4...O-ring, 5...semi-permeable wind
Claims (13)
備え、この反応相の中に被検出物質と結合する前後で蛍
光特性が変化する特徴を持つ抗体を含有させ、この反応
相を被検出溶液中に浸して蛍光特性の変化を測定する場
合に、光ファイバーを通して励起光および出力光の伝達
を行なうことを特徴とする免疫的測定方法。(1) The end of the optical fiber is equipped with a reaction phase covered with a semi-permeable membrane, and this reaction phase contains an antibody whose fluorescence characteristics change before and after binding to the target substance. 1. An immunoassay method characterized by transmitting excitation light and output light through an optical fiber when measuring changes in fluorescence characteristics by immersion in a detection target solution.
備え、この反応相の中に、被検出物質と結合する抗体お
よび蛍光プローブで標識した被検出物質を含有させ、こ
の反応相を被検出溶液中に浸した際、半透膜を通過して
反応相内に入った被検出物質と蛍光プローブで標識した
被検出物質との抗体結合の競合反応に伴う蛍光特性の変
化を測定する場合に光ファイバーを通して励起光および
出力光の伝達を行なうことを特徴とする免疫的測定方法
。(2) A reaction phase covered with a semi-permeable membrane is provided at the end of the optical fiber, and the reaction phase contains an antibody that binds to the analyte and a analyte labeled with a fluorescent probe. When immersed in a detection solution, changes in fluorescence characteristics are measured due to the competitive reaction of antibody binding between the detection substance that has passed through the semipermeable membrane and entered the reaction phase and the detection substance labeled with a fluorescent probe. An immunoassay method characterized by transmitting excitation light and output light through an optical fiber.
ｐＨ６〜９の緩衝溶液で満たされていることを特徴とす
る請求項１又は２記載の免役的測定方法。(3) The immunoassay method according to claim 1 or 2, wherein the reaction phase covered with a semipermeable membrane at the end of the optical fiber is filled with a buffer solution having a pH of 6 to 9.
体を用いることを特徴とする請求項１記載の免疫的測定
方法。(4) The immunoassay method according to claim 1, characterized in that an antibody whose fluorescence intensity decreases upon binding to a substance to be detected is used.
抗体と結合することによって増強することを特徴とする
請求項２記載の免疫的測定方法。(5) The immunoassay method according to claim 2, wherein the fluorescence intensity of the target substance labeled with a fluorescent probe is enhanced by binding to an antibody.
であることを特徴とする請求項２記載の免疫的測定方法
。(6) The immunoassay method according to claim 2, wherein the fluorescent probe that labels the substance to be detected is a dansyl group.
物質と結合する抗体がポリクローナル抗体であることを
特徴とする請求項１又は２記載の免疫的測定方法。(7) The immunoassay method according to claim 1 or 2, wherein the antibody that binds to the substance to be detected and the substance to be detected labeled with a fluorescent probe is a polyclonal antibody.
物質と結合する抗体がモノクローナル抗体であることを
特徴とする請求項１又は２記載の免疫的測定方法。(8) The immunoassay method according to claim 1 or 2, wherein the antibody that binds to the substance to be detected and the substance to be detected labeled with a fluorescent probe is a monoclonal antibody.
徴とする請求項１記載の免疫的測定方法。(9) The immunoassay method according to claim 1, wherein the substance to be detected is trinitrotoluene.
ン又はエフェドリンであることを特徴とする請求項２記
載の免疫的測定方法。(10) The immunoassay method according to claim 2, wherein the substance to be detected is methamphetamine, amphetamine, or ephedrine.
特徴とする免疫センサー。(11) An immunosensor characterized by using the immunoassay method according to claim 1.
特徴とする免疫センサー。(12) An immunosensor characterized by using the immunoassay method according to claim 2.
ァイバー部と脱着が可能であることを特徴とする請求項
１１又は１２項記載の免疫センサ。(13) The immunosensor according to claim 11 or 12, wherein the reaction phase provided at the end of the optical fiber is removable from the optical fiber portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1182918A JPH07113638B2 (en) | 1989-07-14 | 1989-07-14 | Immunoassay method and immunosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1182918A JPH07113638B2 (en) | 1989-07-14 | 1989-07-14 | Immunoassay method and immunosensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0346564A true JPH0346564A (en) | 1991-02-27 |
| JPH07113638B2 JPH07113638B2 (en) | 1995-12-06 |
Family
ID=16126660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1182918A Expired - Fee Related JPH07113638B2 (en) | 1989-07-14 | 1989-07-14 | Immunoassay method and immunosensor |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07113638B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993013418A1 (en) * | 1991-12-20 | 1993-07-08 | Ibiden Co., Ltd. | Fluorescent immunity measuring instrument |
| US5523845A (en) * | 1994-02-04 | 1996-06-04 | Biosensor Laboratories Co., Ltd. | A fiber optic device for measuring liquids which are drawn into an end of the device to a predetermined distance from the end of the optical fibers |
-
1989
- 1989-07-14 JP JP1182918A patent/JPH07113638B2/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993013418A1 (en) * | 1991-12-20 | 1993-07-08 | Ibiden Co., Ltd. | Fluorescent immunity measuring instrument |
| US5449625A (en) * | 1991-12-20 | 1995-09-12 | Ibiden Co., Ltd. | Optical fiber based fluorescent immunoassay apparatus |
| US5523845A (en) * | 1994-02-04 | 1996-06-04 | Biosensor Laboratories Co., Ltd. | A fiber optic device for measuring liquids which are drawn into an end of the device to a predetermined distance from the end of the optical fibers |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH07113638B2 (en) | 1995-12-06 |
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|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |