JPH0337703B2 - - Google Patents
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- Publication number
- JPH0337703B2 JPH0337703B2 JP7238082A JP7238082A JPH0337703B2 JP H0337703 B2 JPH0337703 B2 JP H0337703B2 JP 7238082 A JP7238082 A JP 7238082A JP 7238082 A JP7238082 A JP 7238082A JP H0337703 B2 JPH0337703 B2 JP H0337703B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- hydrogen peroxide
- pretreatment
- pretreatment method
- polishing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 68
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims description 22
- 230000000694 effects Effects 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 12
- 229910052697 platinum Inorganic materials 0.000 claims description 11
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 9
- 238000002203 pretreatment Methods 0.000 claims description 9
- 229910052709 silver Inorganic materials 0.000 claims description 9
- 239000004332 silver Substances 0.000 claims description 9
- 238000005238 degreasing Methods 0.000 claims description 8
- 238000005498 polishing Methods 0.000 claims description 7
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- 230000032683 aging Effects 0.000 claims description 6
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 6
- 238000010306 acid treatment Methods 0.000 claims description 5
- 229910001651 emery Inorganic materials 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 3
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 2
- 229910017604 nitric acid Inorganic materials 0.000 claims description 2
- 239000008363 phosphate buffer Substances 0.000 claims 1
- 238000003672 processing method Methods 0.000 claims 1
- 230000000087 stabilizing effect Effects 0.000 claims 1
- 239000012528 membrane Substances 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 10
- 108010093096 Immobilized Enzymes Proteins 0.000 description 10
- 229940088598 enzyme Drugs 0.000 description 10
- 230000007774 longterm Effects 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 230000006866 deterioration Effects 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 230000004044 response Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000004043 responsiveness Effects 0.000 description 3
- 238000004381 surface treatment Methods 0.000 description 3
- 108010015776 Glucose oxidase Proteins 0.000 description 2
- 239000004366 Glucose oxidase Substances 0.000 description 2
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 2
- 238000003891 environmental analysis Methods 0.000 description 2
- 229940116332 glucose oxidase Drugs 0.000 description 2
- 235000019420 glucose oxidase Nutrition 0.000 description 2
- 239000012212 insulator Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- MUMZUERVLWJKNR-UHFFFAOYSA-N oxoplatinum Chemical compound [Pt]=O MUMZUERVLWJKNR-UHFFFAOYSA-N 0.000 description 1
- 229910052763 palladium Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229910003446 platinum oxide Inorganic materials 0.000 description 1
- 229920005597 polymer membrane Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/404—Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid, e.g. Clark-type oxygen sensors
- G01N27/4045—Cells with anode, cathode and cell electrolyte on the same side of a permeable membrane which separates them from the sample fluid, e.g. Clark-type oxygen sensors for gases other than oxygen
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrodes For Compound Or Non-Metal Manufacture (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Description
【発明の詳細な説明】
本発明は、固定化酵素膜を用いる酵素電極の一
種である過酸化水素電極（過酸化水素検出用隔膜
被覆電極）の活性が長期使用に伴ない劣化するた
め、これを改善し長期間安定で、かつ応答性の高
い電極を得るための表面処理法に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention is aimed at solving the problem that the activity of a hydrogen peroxide electrode (diaphragm-coated electrode for detecting hydrogen peroxide), which is a type of enzyme electrode using an immobilized enzyme membrane, deteriorates with long-term use. This paper relates to a surface treatment method for improving electrodes that are stable for a long period of time and have high responsiveness.
酵素電極は酵素と電極とを一体化したものであ
り、選択的、迅速、正確等の長所を有するため、
臨床化学分析、環境分析などの分野で広く用いら
れている。酵素電極を構成する電極としての過酸
化水素電極は、酵素の触媒作用により生成した過
酸化水素を白金などを用いたアノードで酸化し、
その酸化電流値から特定成分を定量する目的で使
用されている。この種の電極は一般に高感度およ
び高寿命であることが望まれる。 Enzyme electrodes integrate enzymes and electrodes, and have the advantages of being selective, rapid, and accurate.
Widely used in fields such as clinical chemistry analysis and environmental analysis. The hydrogen peroxide electrode that constitutes the enzyme electrode oxidizes hydrogen peroxide produced by the catalytic action of an enzyme with an anode made of platinum or the like.
It is used for the purpose of quantifying specific components from the oxidation current value. It is generally desired that this type of electrode has high sensitivity and long life.
過酸化水素の濃度の計測法としては、白金をア
ノード、銀をカソードとして使用し、両電極間に
一定電圧を印加しつつ電解電流を測定し、検量線
法あるいは校正法により、過酸化水素を決定する
ものが多い。白金極の電位を銀極に対して0.6V
以上印加することにより、電解電流は過酸化水素
の拡散律速となる。この計測法による過酸化水素
電極は長期間の使用に伴い、電極の活性低下が認
められる場合が多い。活性度の低下は精度の低下
と検出限界能の悪化をもたらす。劣化が著しい場
合は校正法を適用できなくなる。 To measure the concentration of hydrogen peroxide, use platinum as an anode and silver as a cathode, measure the electrolytic current while applying a constant voltage between the two electrodes, and measure the concentration of hydrogen peroxide using the calibration curve method or calibration method. There are many decisions to be made. The potential of the platinum electrode is 0.6V with respect to the silver electrode.
By applying the above amount, the electrolytic current becomes rate-limited by the diffusion of hydrogen peroxide. Hydrogen peroxide electrodes based on this measurement method often show a decrease in electrode activity after long-term use. A decrease in activity leads to a decrease in accuracy and deterioration of detection limit capability. If the deterioration is significant, the calibration method cannot be applied.
本発明は、上述の欠点を除去し、より高感度で
安定な過酸化水素電極とするための電極表面処理
法を提供することを目的とする。 An object of the present invention is to provide an electrode surface treatment method that eliminates the above-mentioned drawbacks and provides a more sensitive and stable hydrogen peroxide electrode.
活性度の劣化は、白金極には常に一定の電位が
印加されているので、長期間の使用に伴い白金ア
ノード表面に白金酸化物が生成したり、反応液中
に溶存している有機物、無機物が吸着したりする
ためと考えられている。この発明者らはこの点に
着目し、過酸化水素電極の活性度の低下を小さく
し、長期安定な電極を提供するための表面処理法
を見い出した。 Deterioration of activity is caused by the formation of platinum oxide on the surface of the platinum anode due to long-term use, as a constant potential is always applied to the platinum electrode, and organic and inorganic substances dissolved in the reaction solution. It is thought that this is due to the adsorption of The inventors focused on this point and discovered a surface treatment method for reducing the decrease in activity of the hydrogen peroxide electrode and providing a long-term stable electrode.
次に本発明の実施例を図面に基づいて詳細に説
明する。 Next, embodiments of the present invention will be described in detail based on the drawings.
対象とする過酸化水素電極は第１図に示されて
いる。第１図において、１はアノードとしての白
金電極、２は電極支持絶縁体、３はカソードとし
ての銀電極である。白金電極１は銀電極３の中心
部に設けられている。４および５はそれぞれ白金
電極１および銀電極３から導出されたリード線で
ある。このように構成された過酸化水素電極は、
第１Ａ図に示すように、固定化酵素膜装着ガイド
６に収容され、固定化酵素膜５が取付けられて、
酵素電極として使用される。なお、７は０−リン
グである。この第１Ａ図に示した酵素電極は図示
していない測定セル部に固定され、白金電極１と
銀電極３との間に0.6〜0.8Vの範囲の一定電圧が
印加される。 The hydrogen peroxide electrode of interest is shown in FIG. In FIG. 1, 1 is a platinum electrode as an anode, 2 is an electrode supporting insulator, and 3 is a silver electrode as a cathode. The platinum electrode 1 is provided at the center of the silver electrode 3. 4 and 5 are lead wires led out from the platinum electrode 1 and the silver electrode 3, respectively. The hydrogen peroxide electrode configured in this way is
As shown in FIG. 1A, the immobilized enzyme membrane 5 is accommodated in the immobilized enzyme membrane mounting guide 6 and attached.
Used as an enzyme electrode. Note that 7 is a 0-ring. The enzyme electrode shown in FIG. 1A is fixed to a measuring cell section (not shown), and a constant voltage in the range of 0.6 to 0.8 V is applied between the platinum electrode 1 and the silver electrode 3.
過酸化水素電極の前処理法のフローチヤートを
第２図に示す。まず、第１図に示すように成型し
た電極の表面を粗いエメリーペーパー（＃300〜
400程度）で粗削りし、細かいエメリーペーパー
（＃1200〜1500程度）で仕上げを行なう（研磨工
程１１）。次の工程の超音波洗浄は表面に付着し
たエメリーペーパーの粒子、ゴミ等を落すための
操作である。（超音波洗浄工程１２）。また表面の
有機物等を除去するため重曹により充分脱脂を行
なう（脱脂工程１３）。さらに超音波洗浄を行な
い（超音波洗浄工程１４）、硝酸に電極表面を浸
し、他金属粉末等を溶解し取り除く（酸処理工程
１５）。また長期使用により生成する酸化物等の
影響を低減するため電極を燐酸塩緩衝液（PH8.5）
に浸し、0.6〜0.8Vの一定電圧を印加し、エージ
ングを行なう（エージング工程１６）。これによ
り電極感度は長期の使用に渡り安定し、高寿命が
得られる。本発明につき第１図に示した型の過酸
化水素電極を用いた場合の実験結果を示し説明す
る。 A flowchart of the pretreatment method for the hydrogen peroxide electrode is shown in FIG. First, as shown in Figure 1, the surface of the molded electrode was coated with rough emery paper (#300~
400) and finish with fine emery paper (#1200 to 1500) (polishing step 11). The next step, ultrasonic cleaning, is an operation to remove emery paper particles, dirt, etc. that have adhered to the surface. (Ultrasonic cleaning step 12). Further, in order to remove organic substances on the surface, sufficient degreasing is performed using baking soda (degreasing step 13). Further, ultrasonic cleaning is performed (ultrasonic cleaning step 14), and the electrode surface is immersed in nitric acid to dissolve and remove other metal powders (acid treatment step 15). In addition, in order to reduce the effects of oxides etc. generated during long-term use, the electrodes are placed in a phosphate buffer solution (PH8.5).
A constant voltage of 0.6 to 0.8 V is applied to perform aging (aging step 16). As a result, the electrode sensitivity remains stable over long periods of use, resulting in a long service life. The present invention will be explained by showing experimental results using a hydrogen peroxide electrode of the type shown in FIG.
第３図は前処理を行つた過酸化水素電極を透過
性膜（たとえば多孔質の高分子膜）とともに反応
セル部に装着し、ポーラログラフ法により過酸化
水素溶液を測定した実験結果である。第３図に示
すように、前処理を行うことにより、過酸化水素
溶液に対し良好な直線性が得られた。また、電流
−電圧変換器を用いて同様な測定を行つた結果、
第４図に示すように、過酸化水素稀薄溶液につい
ても優れた直線性が得られた。 FIG. 3 shows the results of an experiment in which a pretreated hydrogen peroxide electrode was attached to a reaction cell together with a permeable membrane (for example, a porous polymer membrane), and a hydrogen peroxide solution was measured by the polarographic method. As shown in FIG. 3, by performing the pretreatment, good linearity with respect to the hydrogen peroxide solution was obtained. Also, as a result of similar measurements using a current-voltage converter,
As shown in FIG. 4, excellent linearity was also obtained with the dilute hydrogen peroxide solution.
第５図は、過酸化水素溶液を反応セル部に注入
したときの応答性を示すものである。第５図のよ
うに、前処理を行つた電極は過酸化水素濃度に対
し応答が極めて早く、90％応答時間定常値の90％
値に達する時間）は注入後５秒以内である。また
第６図は電極に固定化酵素（グルコースオキシタ
ーゼ）膜を装着した場合（第１Ａ図の酵素電極）
のグルコース溶液に対する応答性である。固定化
酵素膜を装着した場合でも、いずれのグルコース
濃度（500mg／dl、300mg／dl、100mg／dl）につ
いて応答は非常に早く、90％応答時間は10秒以内
である。これに対し、第７図に前処理（重曹脱
脂）をしない電極のグルコース溶液（500mg／dl、
300mg／dl、100mg／dl）に対する応答性を示す。
第６図と第７図との比較から、重曹脱脂を行うこ
とにより、固定化酵素膜を装着した場合でも、良
好な応答性が得られることがわかる。 FIG. 5 shows the response when a hydrogen peroxide solution was injected into the reaction cell section. As shown in Figure 5, the pretreated electrode responds extremely quickly to the hydrogen peroxide concentration, with a 90% response time of 90% of the steady value.
The time to reach the value is within 5 seconds after injection. Figure 6 shows a case where an immobilized enzyme (glucose oxidase) membrane is attached to the electrode (enzyme electrode in Figure 1A).
This is the responsiveness to a glucose solution. Even when equipped with an immobilized enzyme membrane, the response is very fast for all glucose concentrations (500 mg/dl, 300 mg/dl, 100 mg/dl), with a 90% response time of less than 10 seconds. In contrast, Figure 7 shows the glucose solution (500 mg/dl,
300mg/dl, 100mg/dl).
A comparison between FIG. 6 and FIG. 7 shows that by performing baking soda degreasing, good responsiveness can be obtained even when an immobilized enzyme membrane is attached.
第８図は本発明による前処理を行つた過酸化水
素電極の活性度の安定性を示したものである。固
定化グルコースオキシターゼ膜を装着した状態で
グルコース溶液150mg／dlにて測定を行い、３週
間以上にわたる安定性が得られた。また固定化酵
素膜を交換することにより初期値が再現された。
エージング処理を行わない場合には、初期に劣化
が大きく３日間で初期値の約1/4〜1/2となること
があり、エージングを行うことにより高感度で長
期間安定な過酸化水素電極を得ることができる。 FIG. 8 shows the stability of the activity of a hydrogen peroxide electrode subjected to the pretreatment according to the present invention. Measurement was performed using a glucose solution of 150 mg/dl with the immobilized glucose oxidase membrane attached, and stability for over 3 weeks was obtained. In addition, the initial values were reproduced by replacing the immobilized enzyme membrane.
If no aging treatment is performed, the deterioration will be large in the initial stage and the initial value may drop to about 1/4 to 1/2 within 3 days. can be obtained.
本発明方法を実施することにより、、酵素電極
用過酸化水素電極の活性度が安定化するため、過
酸化水素電極および固定化酵素膜の交換の回数を
減少させることができる。このことは過酸化水素
電極、固定化酵素膜の有効利用ができることを意
味する。更には酸化電流と残余電流の比が過酸化
水素電極の活性度の安定化と共に安定するため、
より正確な基質及び過酸化水素の定量が可能とな
る。 By carrying out the method of the present invention, the activity of the hydrogen peroxide electrode for enzyme electrodes is stabilized, so that the number of exchanges of the hydrogen peroxide electrode and the immobilized enzyme membrane can be reduced. This means that the hydrogen peroxide electrode and the immobilized enzyme membrane can be used effectively. Furthermore, the ratio of oxidation current to residual current stabilizes as the activity of the hydrogen peroxide electrode stabilizes.
More accurate determination of substrate and hydrogen peroxide is possible.
過酸化水素検出用隔膜被覆電極は臨床化学分
析、環境分析には広く利用されているが、今度は
有機化学分析、化学プロセス制御の分野にもその
発展が期待できる。本発明方法は他の物質（例え
ば金、パラジウム）の電極にも適用できる。ま
た、酸化物電極に対しても悪同様である。 Diaphragm-coated electrodes for detecting hydrogen peroxide are widely used in clinical chemistry analysis and environmental analysis, but their development is also expected in the fields of organic chemistry analysis and chemical process control. The method of the invention can also be applied to electrodes of other materials (eg gold, palladium). The same applies to oxide electrodes.
以上に説明したように、本発明による過酸化水
素電極の前処理方法は、過酸化水素電極の電極表
面を研磨する研磨工程と、前記電極表面に付着し
ている汚れを落とす超音波洗浄工程と、前記電極
表面に付着している有機物等を除去して脱脂を行
なう脱脂工程と、前記電極表面に付着している金
属粉末等を除去する酸処理工程と、前記電極の活
性度の安定化を図るエージング工程とを有するこ
とによつて、より高感度で安定な過酸化水素電極
を提供することができる。 As explained above, the pretreatment method for a hydrogen peroxide electrode according to the present invention includes a polishing step for polishing the electrode surface of the hydrogen peroxide electrode, and an ultrasonic cleaning step for removing dirt adhering to the electrode surface. , a degreasing step for removing organic matter etc. adhering to the electrode surface and degreasing; an acid treatment step for removing metal powder etc. adhering to the electrode surface; and stabilization of the activity of the electrode. By including the aging step, it is possible to provide a more sensitive and stable hydrogen peroxide electrode.
なお、第２図のフローチヤートにおいて、脱脂
工程は酸処理行程の後に行なつてもよい。 In addition, in the flowchart of FIG. 2, the degreasing step may be performed after the acid treatment step.
第１図は過酸化水素電極の概略断面図、第１Ａ
図は酵素電極の概略断面図、第２図は本発明によ
る前処理方法のフローチヤート、第３図ないし第
８図は本発明方法の効果を説明するための実験結
果を示す特性図である。
１……白金電極、２……絶縁体、３……銀電
極、５……固定化酵素膜。
Figure 1 is a schematic cross-sectional view of a hydrogen peroxide electrode, 1A
The figure is a schematic sectional view of an enzyme electrode, FIG. 2 is a flowchart of the pretreatment method according to the present invention, and FIGS. 3 to 8 are characteristic diagrams showing experimental results for explaining the effects of the method of the present invention. 1... Platinum electrode, 2... Insulator, 3... Silver electrode, 5... Immobilized enzyme membrane.
Claims (1)
前記銀電極の中心部に絶縁して設けられた白金電
極とから成る過酸化水素電極の前処理方法におい
て、前記過酸化水素電極の電極表面を研磨する研
磨工程と、前記電極表面に付着している汚れを落
とす超音波洗浄工程と、前記電極表面に付着して
いる有機物等を除去して脱脂を行う脱脂工程と、
前記電極表面に付着している金属粉末等を除去す
る酸処理工程と、前記電極の活性度の安定化を図
るエージング工程とを有することを特徴とする過
酸化水素電極の前処理方法。 ２ 特許請求の範囲第１項記載の前処理方法にお
いて、研磨工程は、電極表面を粗いエメリーペー
パーで粗削りし、次に細かいエメリーペーパーで
仕上げを行うことより成ることを特徴とする過酸
化水素電極の前処理方法。 ３ 特許請求の範囲第１項または第２項記載の前
処理方法において、脱脂工程は、重曹を用いて行
われることを特徴とする過酸化水素電極の前処理
方法。 ４ 特許請求の範囲第１項ないし第３項記載のい
ずれかの項記載の前処理方法において、酸処理工
程は、硝酸に電極表面を浸して行われることを特
徴とする過酸化水素電極の前処理方法。 ５ 特許請求の範囲第１項ないし第４項記載のい
ずれかの項記載の前処理方法において、エージン
グ工程は、電極を燐酸塩緩衝液に浸し、電極に所
定の電圧を印加して行われることを特徴とする過
酸化水素電極の前処理方法。[Scope of Claims] 1. A method for pretreatment of a hydrogen peroxide electrode comprising a silver electrode as a cathode and a platinum electrode insulated at the center of the silver electrode as an anode. a polishing step for polishing the electrode surface; an ultrasonic cleaning step for removing dirt adhering to the electrode surface; and a degreasing step for removing organic substances and the like adhering to the electrode surface.
A pretreatment method for a hydrogen peroxide electrode, comprising an acid treatment step for removing metal powder etc. adhering to the electrode surface, and an aging step for stabilizing the activity of the electrode. 2. A hydrogen peroxide electrode in the pretreatment method according to claim 1, characterized in that the polishing step consists of roughly polishing the electrode surface with coarse emery paper, and then finishing with fine emery paper. Pretreatment method. 3. A pretreatment method for a hydrogen peroxide electrode according to claim 1 or 2, wherein the degreasing step is performed using baking soda. 4. In the pretreatment method described in any one of claims 1 to 3, the acid treatment step is performed by immersing the electrode surface in nitric acid. Processing method. 5. In the pretreatment method according to any one of claims 1 to 4, the aging step is performed by immersing the electrode in a phosphate buffer and applying a predetermined voltage to the electrode. A method for pretreatment of a hydrogen peroxide electrode, characterized by:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7238082A JPS58189551A (en) | 1982-04-28 | 1982-04-28 | Pretreatment of hydrogen peroxide electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7238082A JPS58189551A (en) | 1982-04-28 | 1982-04-28 | Pretreatment of hydrogen peroxide electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58189551A JPS58189551A (en) | 1983-11-05 |
| JPH0337703B2 true JPH0337703B2 (en) | 1991-06-06 |
Family
ID=13487624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7238082A Granted JPS58189551A (en) | 1982-04-28 | 1982-04-28 | Pretreatment of hydrogen peroxide electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58189551A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2201248B (en) * | 1987-02-24 | 1991-04-17 | Ici Plc | Enzyme electrode sensors |
| JPH0727847B2 (en) * | 1987-06-16 | 1995-03-29 | 昭和電工株式会社 | Method for manufacturing solid electrolytic capacitor |
-
1982
- 1982-04-28 JP JP7238082A patent/JPS58189551A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58189551A (en) | 1983-11-05 |
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