JPH0333314B2 - - Google Patents
Info
- Publication number
- JPH0333314B2 JPH0333314B2 JP59051393A JP5139384A JPH0333314B2 JP H0333314 B2 JPH0333314 B2 JP H0333314B2 JP 59051393 A JP59051393 A JP 59051393A JP 5139384 A JP5139384 A JP 5139384A JP H0333314 B2 JPH0333314 B2 JP H0333314B2
- Authority
- JP
- Japan
- Prior art keywords
- virus
- cells
- cultured
- bovine
- cows
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000283690 Bos taurus Species 0.000 claims description 16
- 241000700605 Viruses Species 0.000 claims description 14
- 241000699800 Cricetinae Species 0.000 claims description 6
- 230000002238 attenuated effect Effects 0.000 claims description 6
- 210000003292 kidney cell Anatomy 0.000 claims 3
- 210000005265 lung cell Anatomy 0.000 claims 2
- 241000700199 Cavia porcellus Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 210000004748 cultured cell Anatomy 0.000 claims 1
- 210000003501 vero cell Anatomy 0.000 claims 1
- 230000005856 abnormality Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 230000000241 respiratory effect Effects 0.000 description 3
- 241000700198 Cavia Species 0.000 description 2
- 241000699670 Mus sp. Species 0.000 description 2
- 206010037660 Pyrexia Diseases 0.000 description 2
- 241000725643 Respiratory syncytial virus Species 0.000 description 2
- 206010061603 Respiratory syncytial virus infection Diseases 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000003928 nasal cavity Anatomy 0.000 description 2
- 229960005486 vaccine Drugs 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000711895 Bovine orthopneumovirus Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010014561 Emphysema Diseases 0.000 description 1
- 206010023644 Lacrimation increased Diseases 0.000 description 1
- 206010024264 Lethargy Diseases 0.000 description 1
- 208000037656 Respiratory Sounds Diseases 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 206010039424 Salivary hypersecretion Diseases 0.000 description 1
- 206010047924 Wheezing Diseases 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000005847 immunogenicity Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 230000004317 lacrimation Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 208000010753 nasal discharge Diseases 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 238000010837 poor prognosis Methods 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 208000026451 salivation Diseases 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Description
本発明は、牛のRSウイルス感染に起因する牛
の呼吸器病を予防するための牛のRSウイルスに
関するもので、牛に接種した場合に起病性がなく
安全であり、かつ野外で本病の感染を防御する免
疫原性を有する弱毒株をうることを目的とする。
牛がRSウイルスに感染すると、牛は発熱、食
欲の減退または廃絶や元気消失、せき、呼吸速
迫、喘鳴、鼻汁の流出、流涙及び流涎など、重篤
な呼吸器症状を発現することが知られている。我
が国では、昭和43年から44年にかけて全国的に大
流行がみられ、以来常在化して例年各地で発生
し、畜産経営に大きな損失を与えているのが現状
である。すなわち、本ウイルスに感染すると、肺
気腫をおこし、細菌の二次感染によつて症状が悪
化して予後不良の牛も生じている。
したがつて、RSウイルスの感染による損害を
なくするために、ワクチンによる予防法の確立が
望まれているが、現在に至るまで有効なワクチン
は開発されていないのが現状である。
本発明は野外における牛RSウイルス自然感染
牛から分離した牛RSウイルスを培養細胞で30℃
で継代を行い、ウイルスを弱毒化したもので、こ
れを接種することにより、牛に免疫を付与するこ
とで、RSウイルスの感染を予防し、発熱その他
の呼吸器症状の発現を防ぐことを特徴とする。
この弱毒株を実施例について説明すると、野外
で呼吸器症状を呈した牛の鼻粘液から牛腎培養細
胞に分利して得た牛RSウイルスを、ハムスター
肺培養細胞に接種して30℃で培養し、かつ同細胞
でウイルスの継代を行いウイルスを増殖させる。
増殖させたウイルスを累代継代し、３回クローニ
ングして牛RSウイルス弱毒株を得たものである。
この弱毒株の安全性を調べるために、これを、
(A) ４週齢マウスの腹腔、皮下、筋肉または、鼻
腔内に接種し、観察した結果、すべての試験マ
ウスについて異常は認められなかつた。
(B) 体重約300ｇのモルモツトの腹腔、皮下、筋
肉または鼻腔内に接種し、観察した結果、すべ
ての試験モルモツトにおいて異常は認められな
かつた。
(C) 体重約50ｇのハムスターの腹腔、皮下、筋肉
または鼻腔内に接種し、観察した結果、すべて
の試験ハムスターに異常は認められなかつた。
以上の試験結果は次表のとおりであつた。
The present invention relates to bovine respiratory syncytial virus (RSV) for preventing bovine respiratory disease caused by bovine RS virus infection. The aim is to obtain an attenuated strain with immunogenicity that protects against infection. When cows are infected with RSV, they can develop severe respiratory symptoms such as fever, decreased or absent appetite, lethargy, cough, rapid breathing, wheezing, nasal discharge, lacrimation, and salivation. Are known. In Japan, there was a nationwide outbreak from 1963 to 1962, and since then it has become endemic and occurs in various places every year, causing great losses to livestock farming. In other words, when cows are infected with this virus, they develop pulmonary emphysema, and the symptoms worsen due to secondary bacterial infection, resulting in some cows having a poor prognosis. Therefore, in order to eliminate the damage caused by RSV infection, it is desired to establish a preventive method using a vaccine, but to date no effective vaccine has been developed. The present invention involves culturing bovine RS virus isolated from cows naturally infected with bovine RS virus in the field at 30°C.
This is a weakened version of the virus that has been subcultured with the virus. By inoculating it with this virus, cows are given immunity, which prevents RSV infection and prevents the development of fever and other respiratory symptoms. Features. To explain this attenuated strain in an example, bovine RS virus obtained by dividing bovine kidney culture cells from the nasal mucus of a cow exhibiting respiratory symptoms in the field was inoculated into hamster lung culture cells and incubated at 30°C. The cells are cultured and the virus is passaged in the same cells to propagate the virus.
The grown virus was passaged and cloned three times to obtain an attenuated strain of bovine RS virus. In order to investigate the safety of this attenuated strain, it was inoculated (A) intraperitoneally, subcutaneously, intramuscularly, or intranasally into 4-week-old mice, and as a result of observation, no abnormalities were observed in any of the test mice. . (B) As a result of inoculating into the abdominal cavity, subcutaneously, muscle, or nasal cavity of guinea pigs weighing approximately 300 g, no abnormalities were observed in any of the tested guinea pigs. (C) As a result of inoculating into the abdominal cavity, subcutaneously, muscle, or nasal cavity of hamsters weighing approximately 50 g, no abnormalities were observed in any of the test hamsters. The above test results are shown in the table below.
【表】
.
２ 28 10 筋肉内 0.2
10２ ７ 異常なし 18.6 22.6
【table】
.
2 28 10 Intramuscular 0.2
10 2 7 No abnormalities 18.6 22.6
Claims (1)
ウイルスを、ハムスター肺培養細胞、ハムスター
肺由来細胞、ハムスター腎培養細胞、モルモツト
腎培養細胞、豚腎培養細胞HmLu−１細胞、
Vero細胞から選んだ培養細胞を用いて30℃で継
代を行い弱毒化した内のRSウイルス弱毒株。1 Bovine RS isolated from cows naturally infected with bovine RS virus
The virus was introduced into cultured hamster lung cells, cultured hamster lung cells, cultured hamster kidney cells, cultured guinea pig kidney cells, cultured pig kidney cells HmLu-1 cells,
An attenuated strain of RS virus that has been attenuated by passage at 30℃ using cultured cells selected from Vero cells.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5139384A JPS60196187A (en) | 1984-03-16 | 1984-03-16 | Attenuated strain of bovine rs virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5139384A JPS60196187A (en) | 1984-03-16 | 1984-03-16 | Attenuated strain of bovine rs virus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60196187A JPS60196187A (en) | 1985-10-04 |
| JPH0333314B2 true JPH0333314B2 (en) | 1991-05-16 |
Family
ID=12885691
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5139384A Granted JPS60196187A (en) | 1984-03-16 | 1984-03-16 | Attenuated strain of bovine rs virus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60196187A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4911829A (en) * | 1972-05-12 | 1974-02-01 | ||
| JPS4940925A (en) * | 1972-08-26 | 1974-04-17 | ||
| JPS50154415A (en) * | 1974-03-25 | 1975-12-12 | ||
| JPS5381617A (en) * | 1976-12-24 | 1978-07-19 | Kitasato Inst | Virus vaccine for infectious spleen breakdown of trout |
| JPS53121924A (en) * | 1977-02-09 | 1978-10-24 | Merck & Co Inc | Respiratory syncytial vaccine |
-
1984
- 1984-03-16 JP JP5139384A patent/JPS60196187A/en active Granted
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS4911829A (en) * | 1972-05-12 | 1974-02-01 | ||
| JPS4940925A (en) * | 1972-08-26 | 1974-04-17 | ||
| JPS50154415A (en) * | 1974-03-25 | 1975-12-12 | ||
| JPS5381617A (en) * | 1976-12-24 | 1978-07-19 | Kitasato Inst | Virus vaccine for infectious spleen breakdown of trout |
| JPS53121924A (en) * | 1977-02-09 | 1978-10-24 | Merck & Co Inc | Respiratory syncytial vaccine |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60196187A (en) | 1985-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |