JPH03262478A - Culture device - Google Patents
Culture deviceInfo
- Publication number
- JPH03262478A JPH03262478A JP5792790A JP5792790A JPH03262478A JP H03262478 A JPH03262478 A JP H03262478A JP 5792790 A JP5792790 A JP 5792790A JP 5792790 A JP5792790 A JP 5792790A JP H03262478 A JPH03262478 A JP H03262478A
- Authority
- JP
- Japan
- Prior art keywords
- membrane
- culture
- porous membrane
- gas
- culture device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000012528 membrane Substances 0.000 claims abstract description 34
- 230000002209 hydrophobic effect Effects 0.000 claims abstract description 13
- 244000005700 microbiome Species 0.000 claims abstract description 3
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 3
- 239000011148 porous material Substances 0.000 claims description 10
- 238000005273 aeration Methods 0.000 claims 2
- 238000004113 cell culture Methods 0.000 claims 1
- 239000006260 foam Substances 0.000 abstract description 5
- 239000002518 antifoaming agent Substances 0.000 abstract description 2
- 239000012808 vapor phase Substances 0.000 abstract 2
- 239000007789 gas Substances 0.000 description 21
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 238000000605 extraction Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000630 rising effect Effects 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000011295 pitch Substances 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用公社〕
本発明は、微生物や細胞の液中通気培養における発泡に
よる問題を解消し、気体のみを抜き出すようにした培養
装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Corporation] The present invention relates to a culture device that eliminates problems caused by foaming in submerged aerated culture of microorganisms and cells and extracts only gas.
従来の装置ハ、＊ｂ平０１−６７９１４号公報に記載の
ように破泡を目的として行われていた。The conventional apparatus C was used for the purpose of breaking bubbles as described in *b Publication No. 01-67914.
またこの方法では、消抱層に破水剤を塗布していなけれ
ばならず、時間の経過とともにこの疎水剤も減少してい
くという問題があった。In addition, this method requires that a water-breaking agent be applied to the sorption layer, and there is a problem in that this hydrophobic agent also decreases over time.
上記従来技術は、・数顛のピッチで編んだ格子状のよう
な物に疎水性のシリコングリス等で表面を覆い、発泡し
た泡に触れさせて破泡させるという破泡を目的とした発
明であった。よって、数日〜数箇月培養を行うと表面に
塗布されたシリコノグリスは減少し、最後には破泡をし
なくなるという問題があった。The above-mentioned conventional technology is an invention aimed at breaking foam by covering the surface of a lattice-like material woven at several pitches with hydrophobic silicone grease, etc., and letting it touch the foamed foam to break it. there were. Therefore, after culturing for several days to several months, the amount of silicono grease applied to the surface decreases, and eventually bubbles no longer break.
本発明の目的は、半永久的に使用できメンテナンスフリ
ーとなる培養装置を提供することにある。An object of the present invention is to provide a culture device that can be used semi-permanently and is maintenance-free.
上記目的を達成するために、液中通気している培養槽の
気相部を、疎水性がありしかも孔径が例えば１０μＵ以
上の多孔質の膜で覆うように構成したものである。つま
り、鉄膜で気相部を密閉するように取り可けたものであ
る。In order to achieve the above object, the gas phase part of the culture tank which is aerated in the liquid is covered with a hydrophobic and porous membrane having a pore size of, for example, 10 μU or more. In other words, it was possible to seal the gas phase part with an iron membrane.
さらに、上記膜を複数段多層式にするためには、気相部
に上記膜を複数枚一定の間隔をおいて重ねて設置したも
のである。Furthermore, in order to make the above-mentioned membrane multi-stage and multi-layered, a plurality of the above-mentioned membranes are placed one on top of the other at regular intervals in the gas phase section.
培養液中に通気された気体は、上方に移動して発泡を行
う。泡は徐々Ｉこ上方に移動して行き、孔径１０μＵ以
上の孔を多く持つ疎水性多孔質の膜に接触する。泡は親
水性であるため鉄膜に接触すると破泡してしまい、泡の
中の気体のみ膜を通過する。The gas aerated into the culture solution moves upward and causes foaming. The bubbles gradually move upward and come into contact with a hydrophobic porous membrane having many pores with a pore diameter of 10 μU or more. Since the bubbles are hydrophilic, they break when they come into contact with the iron membrane, and only the gas inside the bubbles passes through the membrane.
また、通気流量の多い場合は、孔径の大きい疏水性多孔
質膜を複数段多層式にすることにより、水分の上方への
移動を防く゛ことができる。Further, when the ventilation flow rate is large, upward movement of moisture can be prevented by using a multi-stage, multi-layered hydrophobic porous membrane with large pore diameters.
し、下、本発明の一実施例′５ｒ：第１図により説明す
る。１は培養槽４の気相部を覆うように設Ｇすた例えば
孔径１０μ顛以上の多孔質で疎水性の膜、２は循環用気
体の供給によって発生した泡、３は培養液、４はエアー
リフト力により培養液３の混合を行うエアーリフト型の
培養槽である。そして、本培１ＰＮ４内の培＄０３の清
報は、培養槽４側面から取り付けたＰＨセンサー１１．
Ｄｏセンサー１２゜温度センサー１３を用いて得られる
ようになっている。Below, one embodiment of the present invention '5r will be explained with reference to FIG. 1 is a porous and hydrophobic membrane with a pore diameter of 10 μm or more, which is arranged to cover the gas phase of the culture tank 4, 2 is a bubble generated by supplying circulating gas, 3 is a culture solution, and 4 is a membrane. This is an air lift type culture tank that mixes the culture solution 3 using air lift force. Then, the latest information on the culture medium $03 in the main culture 1PN4 is detected by the PH sensor 11. attached from the side of the culture tank 4.
The temperature can be obtained using a Do sensor 12° temperature sensor 13.
培養槽４下部の循環用気体供給管當８から気体が供給さ
れるこち１こより、培養液３は混合される。The culture solution 3 is mixed through the gas supplied from the circulating gas supply pipe 8 at the bottom of the culture tank 4.
また、細胞の要求酸素は、培養槽気体供給管７から送り
込まれる。これらの供給された気体は、培ｌＩ液３中を
上方に移動しながら泡２となる。泡２は自然破泡しない
かぎり無くならないので、［１まで上昇する。！！！１
は、例えば孔径３０μＵのＰＴＦＢ（ポリテトラフルオ
ロエチレン）製の多孔膜を使用する。泡２は膜ｌに接触
すると、さらに下方より上昇してくる泡２の圧力により
泡２の大きさよりはるかに小さい孔径を持つ膜ｌに接触
して破泡する。そして、泡２内の気体は膜ｌを通過し、
気体抜き出し管９より培養槽４外に抜き出される。また
、ここで使用した上記膜ｌは、第２図（ａ） 、 （ｂ
）に示した複数段多層式（スペーサーｌＯを用いて膜１
を等間隔に設置したもの）の膜を用いて培養を行っても
よい。この場合、膜１は例えば孔径が５００μＵ以上の
ＰＴＦＢ製の多孔質膜を用いる。液面より上昇してくる
泡２は、第１段目の膜を通過するものがあるかもしれな
いが、第２段目、第３段目となるにつれて泡は全て破泡
され最上段では気体のみとなり、気体抜き出し管９より
気体は培養槽４外に抜き出される。Further, the oxygen required by the cells is sent from the culture tank gas supply pipe 7. These supplied gases become bubbles 2 while moving upward in the culture medium 3. Since bubble 2 will not disappear unless it spontaneously breaks, it will rise to [1]. ! ! ! 1
For example, a porous membrane made of PTFB (polytetrafluoroethylene) with a pore diameter of 30 μU is used. When the bubbles 2 come into contact with the membrane 1, the pressure of the bubbles 2 rising from below causes them to come into contact with the membrane 1, which has a pore diameter much smaller than the size of the bubbles 2, and break the bubbles. Then, the gas in the bubble 2 passes through the membrane l,
The gas is extracted from the culture tank 4 through the gas extraction pipe 9. In addition, the above-mentioned membrane l used here is shown in Fig. 2 (a) and (b).
) shown in the multi-stage multilayer system (membrane 1 using spacer lO)
Culture may also be performed using membranes (equally spaced membranes). In this case, the membrane 1 is, for example, a porous membrane made of PTFB with a pore size of 500 μU or more. Some of the bubbles 2 rising from the liquid level may pass through the first stage membrane, but as they reach the second and third stages, all the bubbles are broken and the top stage becomes gas. The gas is extracted from the culture tank 4 through the gas extraction pipe 9.
本実施例）こよれば、破泡が完全に行なわれ、メンテナ
ンスフリーの培養装置が得られる。According to this embodiment, bubbles are completely broken and a maintenance-free culture device is obtained.
本発明ｉこよれば、細胞によっては阻害物質となり得る
消泡剤を添加することなく、メンテナンスフリーで、し
かも安価で培養が行えるという多大な効果がある。According to the present invention, there is a great effect that culture can be carried out maintenance-free and at low cost without adding an antifoaming agent that can be an inhibitory substance depending on the cells.
第１図は本発明の一実施例の断面図、第２図（ａ） 、
（ｂ）は複数段多層式の膜の概略図である。
１・・・・・・膜、２・・・・・・泡、３・・・・・・
培養液、４・・・・・・培養槽、５・・・・・・温調ジ
ャケット、６・・・・・・細胞循環管、７・・・・・・
培養槽気体供給管、８・・・・・・循環用気体供給管、
９・・−・・・気体抜き出し管、】Ｏ・・・・・・スペ
ーサーFIG. 1 is a sectional view of one embodiment of the present invention, FIG. 2(a),
(b) is a schematic diagram of a multi-stage multilayer membrane. 1...Membrane, 2...Bubble, 3...
Culture solution, 4... Culture tank, 5... Temperature control jacket, 6... Cell circulation tube, 7...
Culture tank gas supply pipe, 8... Circulation gas supply pipe,
9...Gas extraction pipe,]O...Spacer
Claims (1)
培養槽中の気相部を疎水性の多孔質膜で覆うようにした
ことを特徴とする培養装置。 ２、前記疎水性の多孔質膜が、１０μｍ以上の孔径を有
する疎水性のポリテトラフルオロエチレンで構成したこ
とを特徴とする請求項第１様の記載の培養装置。 ３、前記培養槽の気相部を、疎水性の多孔質膜に空気相
を持たせ複数段の多層式に構成したことを特徴とする請
求項第１項の記載の培養装置。 ４、前記培養槽の気成部を、疎水性の多孔質膜に空気相
を持たせ複数段の多層式に構成すると共に、膜の多孔径
を各段毎に変えたことを特徴とする請求項第１項記載の
培養装置。[Claims] 1. In a microorganism or cell culture device that performs submerged aeration,
A culture device characterized in that a gas phase part in a culture tank is covered with a hydrophobic porous membrane. 2. The culture device according to claim 1, wherein the hydrophobic porous membrane is made of hydrophobic polytetrafluoroethylene having a pore size of 10 μm or more. 3. The culture device according to claim 1, wherein the gas phase portion of the culture tank is configured in a multi-layered structure with a plurality of stages in which a hydrophobic porous membrane has an air phase. 4. A claim characterized in that the aeration part of the culture tank is configured in a multi-layered structure with a plurality of stages in which a hydrophobic porous membrane has an air phase, and the pore diameter of the membrane is changed for each stage. The culture device according to item 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5792790A JPH03262478A (en) | 1990-03-12 | 1990-03-12 | Culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5792790A JPH03262478A (en) | 1990-03-12 | 1990-03-12 | Culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03262478A true JPH03262478A (en) | 1991-11-22 |
Family
ID=13069640
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5792790A Pending JPH03262478A (en) | 1990-03-12 | 1990-03-12 | Culture device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03262478A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018019615A (en) * | 2016-08-02 | 2018-02-08 | 株式会社日立製作所 | Single-use culture device and culture method |
| DE102018102703A1 (en) * | 2018-02-07 | 2019-08-08 | Technische Universität Dortmund | Reactor and process |
-
1990
- 1990-03-12 JP JP5792790A patent/JPH03262478A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2018019615A (en) * | 2016-08-02 | 2018-02-08 | 株式会社日立製作所 | Single-use culture device and culture method |
| WO2018025686A1 (en) * | 2016-08-02 | 2018-02-08 | 株式会社日立製作所 | Single-use culture apparatus and culture method |
| DE102018102703A1 (en) * | 2018-02-07 | 2019-08-08 | Technische Universität Dortmund | Reactor and process |
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