JPH03249559A - Method for measuring concentration of material in activated sludge - Google Patents
Method for measuring concentration of material in activated sludgeInfo
- Publication number
- JPH03249559A JPH03249559A JP4773390A JP4773390A JPH03249559A JP H03249559 A JPH03249559 A JP H03249559A JP 4773390 A JP4773390 A JP 4773390A JP 4773390 A JP4773390 A JP 4773390A JP H03249559 A JPH03249559 A JP H03249559A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- activated sludge
- measuring
- amt
- amount
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010802 sludge Substances 0.000 title claims abstract description 19
- 238000000034 method Methods 0.000 title claims description 13
- 239000000463 material Substances 0.000 title abstract 2
- 239000000126 substance Substances 0.000 claims description 12
- 238000005259 measurement Methods 0.000 abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 238000005070 sampling Methods 0.000 abstract 2
- 244000005700 microbiome Species 0.000 description 8
- 239000010865 sewage Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 description 1
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 229960005305 adenosine Drugs 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
Description
【発明の詳細な説明】
Ａ、産業上の利用分野
本発明は、下水処理における活性汚泥法に係わり、特に
活性汚泥中の各種物質濃度を測定する方法に関する。DETAILED DESCRIPTION OF THE INVENTION A. Field of Industrial Application The present invention relates to an activated sludge method in sewage treatment, and particularly to a method for measuring the concentration of various substances in activated sludge.
Ｂ１発明の概要
本発明は、活性汚泥から試料を採取し、この試料の各種
物質濃度を測定する方法において、試料の採取量を５ｍ
ｆｆとすることにより、分析結果の信頼性を確保しなが
ら、試薬使用量および廃棄量を低減することを可能とす
るものである。B1 Summary of the Invention The present invention provides a method for collecting a sample from activated sludge and measuring the concentration of various substances in the sample.
ff, it is possible to reduce the amount of reagents used and the amount of waste while ensuring the reliability of the analysis results.
Ｃ９従来の技術
現在、下水の処理方法としては、微生物の代謝を利用し
た活性汚泥法が主に行われている。この方法において、
浄化能力は、活性汚泥の微生物の量および質に影響され
る。C9 Prior Art Currently, the activated sludge method that utilizes the metabolism of microorganisms is mainly used as a method for treating sewage. In this method,
Purification ability is influenced by the quantity and quality of microorganisms in activated sludge.
したがって下水処理の管理にあたって、汚泥中機生物の
量および質を把握することが重要となる。Therefore, in managing sewage treatment, it is important to understand the quantity and quality of organisms in sludge.
この指標としては、微生物中の特定物質（アデノシン３
リン酸（ＡＴＰ） 、蛋白質、有機性窒素、多糖類など
）の濃度が有効である。This indicator uses a specific substance (adenosine 3) in microorganisms.
Concentrations of phosphoric acid (ATP), proteins, organic nitrogen, polysaccharides, etc.) are effective.
通常、活性汚泥から試料を一定量採取して所要の物質濃
度を分析し、この分析結果を微生物の量および質の指標
としている。Usually, a certain amount of sample is taken from activated sludge and analyzed for the required substance concentration, and the results of this analysis are used as indicators of the quantity and quality of microorganisms.
Ｄ３発明が解決しようとする課題
活性汚泥は、微生物のフロック（固まり）を形成してお
り、このフロックの大きさおよび分散は極めて変わりや
すい。D3 Problems to be Solved by the Invention Activated sludge forms microbial flocs, and the size and distribution of these flocs are highly variable.
このため、試料の採取量が少ない場合、得られた値の信
頼性が低下する問題点があった。For this reason, there is a problem in that when the amount of sample collected is small, the reliability of the obtained values decreases.
一方、微生物中の物質を測定する場合、通常、その物質
を微生物から取り出すために試料に試薬を添加したうえ
で測定を行っている。On the other hand, when measuring a substance in a microorganism, a reagent is usually added to the sample in order to extract the substance from the microorganism.
このため、試料の採取量が多い場合、これに伴って試薬
の使用量が増加し、コスト面で不利となってしまい、廃
棄の面でも不便であった。また、測定操作の自動化にも
不利であった。Therefore, when a large amount of samples are collected, the amount of reagents used increases, which is disadvantageous in terms of cost and inconvenient in terms of disposal. It was also disadvantageous to automation of measurement operations.
本発明は、このような事情に鑑み、適切な試料採取を行
って活性汚泥中の物質濃度を測定する技術を提供するこ
とを目的とする。In view of such circumstances, an object of the present invention is to provide a technique for measuring the concentration of substances in activated sludge by appropriately collecting samples.
Ｅ１課題を解決するための手段および作用本発明では、
異なる試料の採取量における物質濃度の測定結果を統計
し、試料の採取量と測定結果のばらつきとの関係を分析
した。この結果、試料の採取量として５ｍＲが適当であ
ることが判明した。E1 Means and action for solving the problem In the present invention,
The measurement results of substance concentrations for different amounts of sample collected were analyzed, and the relationship between the amount of sample collected and the variation in measurement results was analyzed. As a result, it was found that 5 mR was appropriate as the amount of sample to be collected.
Ｆ、実施例 以下、図面を用いて、本発明の詳細な説明する。F. Example Hereinafter, the present invention will be explained in detail using the drawings.
第１図は、活性汚泥中のＡＴＰの測定手順を示す。FIG. 1 shows the procedure for measuring ATP in activated sludge.
まず、
試料とトリクロロ酢酸（ＴＣＡ）を等量混合してＡＴＰ
を微生物から抽出する。次に、この混合液を０．５ｍ（
！分取し、緩衝液を４，５ｍｌ注入して希釈する。次に
、この溶液を０．５ｍａ分取し、蛍ルシフェリン・ルシ
フェラーゼ試薬０．５ｍ１２を添加する。そして、この
溶液の発光量を測定することにより、ＡＴＰの濃度を分
析することができる。First, mix equal amounts of the sample and trichloroacetic acid (TCA) to prepare ATP.
is extracted from microorganisms. Next, spread this mixed solution 0.5 m (
! Aliquot it and dilute it by injecting 4.5 ml of buffer. Next, 0.5 ml of this solution is separated, and 0.5 ml of firefly luciferin/luciferase reagent is added thereto. Then, by measuring the amount of light emitted from this solution, the concentration of ATP can be analyzed.
ここで、試料採取量として、０．２５，０．５゜１．２
゜５．１０ｍ１２を選択し、上記の手順によりＡＴＰを
測定した。なお、試料は活性汚泥を十分撹拌して均一化
した状態で採取した。Here, the amount of sample collected is 0.25, 0.5°1.2
5.10 m12 was selected and ATP was measured according to the procedure described above. Note that the sample was collected after the activated sludge was thoroughly stirred and homogenized.
第２図は、この測定結果における同時再現性を示す。図
中の数値は、測定を１０回繰り返したときの変動係数（
％）である。FIG. 2 shows the simultaneous reproducibility of this measurement result. The numerical values in the figure are the coefficient of variation (
%).
生物発光法によるＡＴＰ測定では、変動係数的３％のば
らつきがある。したがって変動係数３％程度のばらつき
は測定結果に影響を及ぼさないと考えられる。この基準
に従えば、試料の採取量として５ｍ１２が適当であると
決定できた。ATP measurement using the bioluminescence method has a variation coefficient of 3%. Therefore, it is considered that a variation with a coefficient of variation of about 3% does not affect the measurement results. According to this standard, it was determined that 5 m12 was an appropriate amount of sample to be collected.
Ｈ１発明の詳細
な説明したように、本発明によれば、活性汚泥中の各種
物質を測定するにあたり、適切な試料の採取量が求まっ
たので、測定の精度が高まり、活性汚泥中の微生物の量
および質の指標としての各種物質濃度の信頼性が向上す
る。As described in detail of the H1 invention, according to the present invention, when measuring various substances in activated sludge, an appropriate amount of sample to be collected is determined, which improves measurement accuracy and improves the accuracy of microorganisms in activated sludge. The reliability of various substance concentrations as indicators of quantity and quality is improved.
また、必要最小限の採取量の試料で測定を行うので、試
薬量を節約でき、経済的に有利である。Furthermore, since the measurement is performed using the minimum necessary amount of sample, the amount of reagents can be saved, which is economically advantageous.
しかも、測定系を小型化することが可能となり、測定操
作の自動化にも資するものと期待できる。Furthermore, it is possible to downsize the measurement system, and it is expected that it will contribute to the automation of measurement operations.
第１図はＡＴＰ測定手順を示す説明図、第２図は汚泥中
ＡＴＰ測定における同時再現性を示す説明図である。
外２名
第
図
第２図
汚泥中ＡＴＰ測定における同時百現性FIG. 1 is an explanatory diagram showing the ATP measurement procedure, and FIG. 2 is an explanatory diagram showing the simultaneous reproducibility in measuring ATP in sludge. Figure 2: Simultaneous permeability in ATP measurement in sludge
Claims (1)
濃度を測定する方法において、 試料の採取量を５ｍｌとしたことを特徴とする活性汚泥
中の物質濃度測定方法。(1) A method for measuring the concentration of substances in activated sludge, characterized in that a sample is collected from activated sludge and the concentration of various substances in the sample is measured, the sample being collected in an amount of 5 ml.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4773390A JPH03249559A (en) | 1990-02-28 | 1990-02-28 | Method for measuring concentration of material in activated sludge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4773390A JPH03249559A (en) | 1990-02-28 | 1990-02-28 | Method for measuring concentration of material in activated sludge |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03249559A true JPH03249559A (en) | 1991-11-07 |
Family
ID=12783543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4773390A Pending JPH03249559A (en) | 1990-02-28 | 1990-02-28 | Method for measuring concentration of material in activated sludge |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03249559A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011211919A (en) * | 2010-03-31 | 2011-10-27 | Aquas Corp | Evaluation method for activity of microorganism, and method for regulating water-based microorganism by using the evaluation method |
-
1990
- 1990-02-28 JP JP4773390A patent/JPH03249559A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011211919A (en) * | 2010-03-31 | 2011-10-27 | Aquas Corp | Evaluation method for activity of microorganism, and method for regulating water-based microorganism by using the evaluation method |
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