JPH03219815A - Method for controlling red disease of seedling of laminaria japonica areschoug - Google Patents
Method for controlling red disease of seedling of laminaria japonica areschougInfo
- Publication number
- JPH03219815A JPH03219815A JP1409990A JP1409990A JPH03219815A JP H03219815 A JPH03219815 A JP H03219815A JP 1409990 A JP1409990 A JP 1409990A JP 1409990 A JP1409990 A JP 1409990A JP H03219815 A JPH03219815 A JP H03219815A
- Authority
- JP
- Japan
- Prior art keywords
- seedling
- chlorine dioxide
- seedlings
- aqueous solution
- disease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 11
- 201000010099 disease Diseases 0.000 title abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract description 21
- 241000015177 Saccharina japonica Species 0.000 title 1
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims abstract description 40
- 239000004155 Chlorine dioxide Substances 0.000 claims abstract description 20
- 235000019398 chlorine dioxide Nutrition 0.000 claims abstract description 20
- 239000007864 aqueous solution Substances 0.000 claims abstract description 10
- 241001262617 Japonica Species 0.000 abstract 4
- 230000034303 cell budding Effects 0.000 abstract 1
- 238000007598 dipping method Methods 0.000 abstract 1
- 244000000010 microbial pathogen Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000013535 sea water Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000195493 Cryptophyta Species 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000000645 desinfectant Substances 0.000 description 3
- 241000589563 Alteromonas sp. Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 244000184734 Pyrus japonica Species 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229910001919 chlorite Inorganic materials 0.000 description 2
- 229910052619 chlorite group Inorganic materials 0.000 description 2
- QBWCMBCROVPCKQ-UHFFFAOYSA-N chlorous acid Chemical compound OCl=O QBWCMBCROVPCKQ-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000288673 Chiroptera Species 0.000 description 1
- XTEGARKTQYYJKE-UHFFFAOYSA-M Chlorate Chemical compound [O-]Cl(=O)=O XTEGARKTQYYJKE-UHFFFAOYSA-M 0.000 description 1
- 206010021033 Hypomenorrhoea Diseases 0.000 description 1
- 241001342551 Macrophyllum Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- GLMQHZPGHAPYIO-UHFFFAOYSA-L azanium;2-hydroxypropane-1,2,3-tricarboxylate;iron(2+) Chemical compound [NH4+].[Fe+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O GLMQHZPGHAPYIO-UHFFFAOYSA-L 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229940005989 chlorate ion Drugs 0.000 description 1
- QBWCMBCROVPCKQ-UHFFFAOYSA-M chlorite Chemical compound [O-]Cl=O QBWCMBCROVPCKQ-UHFFFAOYSA-M 0.000 description 1
- 229940005993 chlorite ion Drugs 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- BHDAXLOEFWJKTL-UHFFFAOYSA-L dipotassium;carboxylatooxy carbonate Chemical compound [K+].[K+].[O-]C(=O)OOC([O-])=O BHDAXLOEFWJKTL-UHFFFAOYSA-L 0.000 description 1
- VTIIJXUACCWYHX-UHFFFAOYSA-L disodium;carboxylatooxy carbonate Chemical compound [Na+].[Na+].[O-]C(=O)OOC([O-])=O VTIIJXUACCWYHX-UHFFFAOYSA-L 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004313 iron ammonium citrate Substances 0.000 description 1
- 235000000011 iron ammonium citrate Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- VISKNDGJUCDNMS-UHFFFAOYSA-M potassium;chlorite Chemical compound [K+].[O-]Cl=O VISKNDGJUCDNMS-UHFFFAOYSA-M 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 235000020989 red meat Nutrition 0.000 description 1
- 239000001054 red pigment Substances 0.000 description 1
- 201000005404 rubella Diseases 0.000 description 1
- UKLNMMHNWFDKNT-UHFFFAOYSA-M sodium chlorite Chemical compound [Na+].[O-]Cl=O UKLNMMHNWFDKNT-UHFFFAOYSA-M 0.000 description 1
- 229960002218 sodium chlorite Drugs 0.000 description 1
- 229960001922 sodium perborate Drugs 0.000 description 1
- 229940045872 sodium percarbonate Drugs 0.000 description 1
- YKLJGMBLPUQQOI-UHFFFAOYSA-M sodium;oxidooxy(oxo)borane Chemical compound [Na+].[O-]OB=O YKLJGMBLPUQQOI-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Cultivation Of Seaweed (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明はマコンブ種苗の赤変病防除方法、更に詳細には
、マコンブの種苗芽胞体に悪影響を及ぼすことなく赤変
病原因菌を除去し、マコンブ種苗の赤変病を防除する方
法に関する。[Detailed Description of the Invention] [Industrial Field of Application] The present invention provides a method for controlling red rot disease in seeds and seedlings of Macombus elegans, and more specifically, a method for removing the bacterium that causes red rot disease without adversely affecting the spore bodies of seeds and seedlings of Macombe. , relates to a method for controlling red rot of Macombus seedlings.
近年、北海道南部を中心に各地で室内培養種苗を用いた
マコンブの促成栽培が盛んに行われている。種苗培養の
概要は次の通りである。毎年８月下旬から９月中旬にか
け母藻を採り、半乾燥し低温処理して遊走子を放出させ
る。遊走子を三角枠に巻いたクレモナ糸（３００ｍ／枠
）に着生させ、培養液（Ｐｒｏｖａｓｏｌ ｉの海水補
強栄養剤：ロＳ＝−〜−西沢−俊、千原光雄編「藻類研
究法」２８３〜２８６頁参照−−−−２１’を加熱滅菌
海水１００１に加えたもの）の入った水槽（これをバッ
トと称する）に入れ、培養を開始する。培養条件は止水
式、通気で、水温８〜１７℃、白色蛍光灯による明期１
２〜１６時間で、培養液は１週間毎に交換する。In recent years, forced cultivation of Macomb using indoor cultured seedlings has been actively carried out in various places, mainly in southern Hokkaido. The outline of seedling culture is as follows. Mother algae are collected from late August to mid-September every year, semi-dried and treated at low temperatures to release zoospores. The zoospores were grown on Cremona thread (300 m/frame) wrapped around a triangular frame, and cultured in a culture solution (Provasol i seawater enrichment nutrient: RoS=-~-Toshi Nishizawa, Mitsuo Chihara, eds. "Algae Research Methods" 283). - See pages 286--21' added to heat-sterilized seawater 1001) into a water tank (this is called a vat) and culture is started. The culture conditions were static, aerated, water temperature 8-17°C, and light period 1 using white fluorescent light.
Culture medium is changed every week for 2-16 hours.
約５０日間の室内培養で葉体が約１ ｃｍになった種苗
を沿岸の施設に移植し、翌年７月に収穫する。After about 50 days of indoor cultivation, the seedlings, whose leaflets have grown to about 1 cm, are transplanted to coastal facilities and harvested in July of the following year.
ところが、昭和５３年頃から各採苗センターで培養開始
２〜５週間目にかけて種苗糸上に微少な赤黒が生じ、時
間とともに拡大し、光斑部分の種苗が脱落するいわゆる
「芽落ち現象」が多発するようになった。培養水温が高
い（１３〜１５℃）場合には一夜にして三角棒全体が赤
色に変色する。However, from around 1981, minute red and black spots appeared on the seedling threads from 2nd to 5th week after the start of cultivation at each seedling collection center, which expanded over time, and the so-called ``bud drop phenomenon'' occurred frequently, in which the seedlings in the light spots fell off. It became so. When the culture water temperature is high (13 to 15°C), the entire triangular rod turns red overnight.
過去１０年間に赤変病害によって投棄されたバット数の
割合は４〜２８％にのぼり、種苗の安定供給に大きな障
害となっている。Over the past 10 years, 4-28% of bats have been discarded due to red rot disease, posing a major obstacle to the stable supply of seeds and seedlings.
本病害の原因菌は非水溶性赤色色素プロデギオシンを産
生じ、他の細菌を溶菌する酵素を産生する海洋細菌Ａｌ
ｔｅｒｏｍｏｎａｓ ｓｐ、である。本原因菌は母藻を
介して培養水槽中に侵入するが、直ちに病害を引き起こ
すことはなく、培養開始後、種苗糸上の一般細菌数がピ
ークに達する２週間目以降に他の細菌を溶菌利用して増
殖を開始し、プロデギオシンを産生じ、種苗系を赤色に
染色するとともに種苗芽胞体に生理障害を与える。The causative bacteria of this disease are marine bacteria Al, which produces the water-insoluble red pigment prodegiosin and produces an enzyme that lyses other bacteria.
teromonas sp. Although this causative bacteria invades the culture tank via mother algae, it does not cause any disease immediately, and lyses other bacteria from the second week after the start of culture, when the number of general bacteria on the seedling threads reaches its peak. It utilizes the enzymes to initiate proliferation, produces prodegiosin, stains the seedling system red, and causes physiological damage to the seedling spore body.
現在までのところ、本病害に対する有効な対策はなく、
本原因菌の増殖下限温度が１０℃であることから、各採
苗センターとも冷凍機を設置し、培養水温を１０℃以下
に保つように努力している。To date, there are no effective measures against this disease.
Since the minimum temperature for the growth of this causative bacterium is 10°C, each seedling collection center has installed a refrigerator and is making efforts to keep the culture water temperature below 10°C.
しかし培養液の検水時には水温が一時的に高くなり、春
夏の発生を見ることが多く、その際は早期に赤身周辺の
種苗糸を切除しているが、冷凍機が故障した場合には被
害が大きくなる。However, when testing the culture solution, the water temperature becomes temporarily high, and outbreaks often occur in spring and summer.In such cases, the seed strings around the red meat are removed early, but if the refrigerator breaks down, The damage will be greater.
従って、本原因菌を効果的に殺菌し、当該赤変病を防除
する方法の開発が望まれていた。Therefore, it has been desired to develop a method for effectively killing the causative bacteria and controlling the red rot disease.
斯かる実情において、本発明者は、上記課題を解決すべ
く、多くの消毒薬についてその効果を試験した。マコン
ブ種苗の培養は止水式で行われるため、消毒処理を行う
と、種苗培養水槽中の細菌相が変動し、水槽の白濁やフ
ロックの発生を引き起し、ひいては種苗の成長に悪影響
を及ぼすおそれがある。従って、本試験では、赤変病原
因菌としてＡｌｔｅｒｏｍｏｎａｓ ｓｐ、 Ｎ（Ｌ
８ Ｒ株を、また本原因菌と同属で、水槽中の主要細菌
相を代表するＡｌｔｅｒｏｍｏｎａｓ ｓｐ、 １０５
５−４株を対照菌として用い、本原因菌を殺菌するが、
対照菌には作用の弱い消毒薬を検索した。その結果は表
１に示すとおりであり、安定化二酸化塩素が本原因菌と
対照菌の殺菌濃度に大きな差があり、本発明の目的にか
なっていることを見出した。Under such circumstances, the present inventor tested the effectiveness of many disinfectants in order to solve the above problems. Since Macomb seedlings are cultured in a still water system, disinfection treatment changes the bacterial flora in the seedling culture tank, causing cloudiness and flocs in the tank, which in turn has a negative impact on the growth of the seedlings. There is a risk. Therefore, in this study, Alteromonas sp, N(L
8 R strain, and Alteromonas sp. 105, which is the same genus as the causative bacteria and represents the main bacterial flora in aquariums.
Strain 5-4 was used as a control bacterium to kill the main causative bacterium, but
We searched for disinfectants that were less effective against control bacteria. The results are shown in Table 1, and it was found that there was a large difference in the sterilizing concentration of stabilized chlorine dioxide between the main causative bacteria and the control bacteria, which met the purpose of the present invention.
以下余白
従って、本発明は、マコンブ種苗を安定化二酸化塩素水
溶液中に浸漬処理することを特徴とするマコンブ種苗の
赤変病防除方法を提供するものである。Margins below Therefore, the present invention provides a method for controlling red blight of Macombus seedlings, which is characterized by immersing the Macombus seedlings in a stabilized chlorine dioxide aqueous solution.
本発明において安定化二酸化塩素とは、亜塩素酸イオン
（ｔＪ口、−）、及び塩素酸イオン（ＩＪ！０３− ）
を主成分とする水溶液で、酸性条件下あるいは光照射、
更には有機物質の存在下において二酸化塩素ガス（ＣＪ
’０２）を徐放するものを言う。In the present invention, stabilized chlorine dioxide refers to chlorite ion (tJ, -) and chlorate ion (IJ!03-)
It is an aqueous solution mainly composed of
Furthermore, in the presence of organic substances, chlorine dioxide gas (CJ
'02) is a sustained release product.
これは、例えば二酸化塩素を水に溶解しアルカリ塩を加
えて安定化するか又は二酸化塩素をアルカリ水溶液に溶
解あるいは亜塩素酸塩水溶液（例えば、亜塩素酸ナトリ
ウム、亜塩素酸カリウム、亜塩素酸カルシウム）に微量
の触媒（例えば、過炭酸ナトリウム、過炭酸カリウム、
過ホウ酸ナトリウム）もしくは微量の酸（塩酸、硫酸、
酢酸、乳酸、クエン酸、燐酸）を加えて製造できる。ま
た、市販の安定化二酸化塩素剤、例えば、オキシツクＡ
（第−製薬製）、ビュロジエン（米国バイオサイド・イ
ンターナショナル製）等を使用することもできる。This can be done, for example, by dissolving chlorine dioxide in water and adding an alkali salt to stabilize it, or by dissolving chlorine dioxide in an aqueous alkaline solution or aqueous chlorite solutions (e.g. sodium chlorite, potassium chlorite, chlorite). Calcium) and trace amounts of catalysts (e.g., sodium percarbonate, potassium percarbonate,
sodium perborate) or trace amounts of acids (hydrochloric acid, sulfuric acid,
It can be manufactured by adding acetic acid, lactic acid, citric acid, phosphoric acid). Additionally, commercially available stabilized chlorine dioxide agents, such as Oxytsuk A
(manufactured by Dai-Pharma), Vulodiene (manufactured by Biocide International, USA), etc. can also be used.
この安定化二酸化塩素の本原因菌及び対照菌に対する消
毒濃度を試験した。試験条件は次のとおりである。The disinfecting concentration of this stabilized chlorine dioxide against the main causative bacteria and control bacteria was tested. The test conditions are as follows.
すなわち、バタトカシトン０．５ｇ、バタトソイトン０
．５ｇ、バクト酵母エキス１．０ｇ、クエン酸鉄アンモ
ニウム０．０１ｇを７５％人工海水１１ （ｐＨ７，５
に調節）に溶解して調製した培地を１２１℃で１５分殺
菌後、菌を接種して２０℃で３日間培養を行った。発育
してきた菌を００．２゜＝１．０に人工海水を用いて稀
釈し、その０．５１ｎｌを消毒薬（検体）の人工海水溶
液〔この安定化二酸化塩素濃度は、オキシツクＡ（第−
製薬製２０．０００ｐｐｍ）を適宜人工海水で希釈した
ものである。例えば、１１００ｐｐとはオキシツクＡ５
ｄを人工海水で１１に希釈したものである。〕４４．５
ｍｆｆに加えて（作用温度１５℃）、所定時間ごとに１
白金耳ずつ上記培地の入った３本の試験管に接種して２
０℃で培養し、各試験管ごとに菌の生育の有無を評価し
た。その結果は表２に示すとおりである。That is, 0.5 g of Batato Kasiton, 0.
．． 5g, Bacto yeast extract 1.0g, iron ammonium citrate 0.01g in 75% artificial seawater 11 (pH 7,5
After sterilizing the culture medium prepared by dissolving the bacteria in 121°C for 15 minutes, the bacteria were inoculated and cultured at 20°C for 3 days. Dilute the grown bacteria to 00.2° = 1.0 using artificial seawater, and add 0.51nl of the diluted solution to an artificial seawater solution of disinfectant (sample).
(20.000 ppm) manufactured by Pharmaceutical Co., Ltd.) was appropriately diluted with artificial seawater. For example, 1100pp means Oxytsuk A5
d diluted to 11 with artificial seawater. ]44.5
mff plus (working temperature 15°C) 1
Inoculate each platinum loop into three test tubes containing the above medium.
The cells were cultured at 0°C, and the presence or absence of bacterial growth was evaluated for each test tube. The results are shown in Table 2.
表２から明らかなように、本原因菌に対しては、１２時
間の処理で５０ ｐｐｍ以上、２４時間の処理で３０ｐ
ｐｍ以上の安定化二酸化塩素濃度において殺菌効果が認
められ、また対照菌に対しては１００ ｐｐｍ、２４時
間の処理によって殺菌効果が認められた。As is clear from Table 2, the concentration of the causative bacteria was 50 ppm or more after 12 hours of treatment, and 30 ppm after 24 hours of treatment.
A bactericidal effect was observed at a stabilized chlorine dioxide concentration of pm or higher, and a bactericidal effect was observed against control bacteria when treated at 100 ppm for 24 hours.
しかしながら、この有効濃度及び処理時間は、マコンブ
種苗生産現場での水の液性、光量の多少、種苗糸による
有効成分の吸着等の環境要因によって変化するので一概
には決められないが、経済的には、３０ ｐｐｍ以上１
００ ｐｐｍ未満の安定化二酸化塩素水溶液を用いて、
マコンブ種苗を１２〜２４時間処理するのが好ましく、
就中特に約８０ｐｐｍで約２４時間処理するのが好まし
い。However, this effective concentration and treatment time cannot be determined unconditionally because they vary depending on environmental factors such as the liquid nature of the water at the production site, the amount of light, and the adsorption of the active ingredient by the seed string, but it is not economical. 30 ppm or more1
Using a stabilized chlorine dioxide aqueous solution of less than 00 ppm,
It is preferable to treat Macomb seedlings for 12 to 24 hours,
Particularly preferred is treatment at about 80 ppm for about 24 hours.
叙上の如く、本発明方法によれば、マコンブの種苗芽胞
体に悪影響を及ぼすことなく、マコンブの赤変病を有利
に防除することができる。As described above, according to the method of the present invention, it is possible to advantageously control the red rot disease of Macelon japonica without having any adverse effect on the seedling spore bodies of Macelon japonica.
次に実施例を挙げて説明する。 Next, an example will be given and explained.
実施例１
０採苗センターで１９８８年９月に培養中のマコンブ種
苗糸に赤変病が発生した。そこで、この赤変病に罹った
種苗糸を培養液を入れたバットに収容し、２０．０００
ｐｐｍの安定化二酸化塩素水溶液を加えて８０ｐｐｆｆ
＋になるようにして２４時間処理した。次いで培養液中
に移して５０日間培養を継続し、海中に移して栽培を行
ったが、種苗糸には赤変病は再発せず、成長もよく、海
中栽培９力月後のマコンブは、安定化二酸化塩素水溶液
無処理の一般群と比較し、葉長がやや短かったが、葉幅
は広く殆んど変りがなかった（第１図及び第２図）。Example 1 In September 1988, red rot disease occurred in the threads of Macomb seedlings that were being cultured at a seedling collection center. Therefore, the seedling threads infected with red rot disease were placed in a vat containing culture solution, and 20,000
Add ppm stabilized chlorine dioxide aqueous solution to 80 ppff
It was treated for 24 hours so that it became positive. Next, they were transferred to a culture solution and continued to be cultured for 50 days, and then transferred to the sea for cultivation, but the red rot disease did not recur on the seedling threads and they grew well. Compared to the general group that was not treated with the stabilized chlorine dioxide aqueous solution, the leaf length was slightly shorter, but the leaf width was wider and remained almost unchanged (Figures 1 and 2).
実施例２
に採苗センターで１９８９年９月に培養中のマコンブ種
苗糸に赤変病が発生した。この赤変病に罹った種苗糸を
１００１容のバットに収容し、実施例１と同様にして８
０ｐｐｍの安定化二酸化塩素水溶液で２４時間処理した
後、海中に移して栽培を行っているが、何ら異常は認め
られない。Example 2 In September 1989, red rot disease occurred in the threads of Macomb seedlings that were being cultured at a seedling center. The seedling threads infected with red rot disease were placed in a 1001 capacity vat, and 8.
After being treated with a 0 ppm stabilized chlorine dioxide aqueous solution for 24 hours, they were moved into the sea and cultivated, but no abnormalities were observed.
実施例３
０採苗センターで１９８９年９〜１０月に培養中のマコ
ンブ種苗糸に赤変病が発生した。この赤変病に罹った種
苗系をバットに収容し、その１槽（Ａ群）には安定化二
酸化塩素を５０ ｐｐｍになるように加えて２５時間処
理し、また他の槽（Ｂ群）には安定化二酸化塩素を８０
ｐｐｍになるように加えて２４時間処理した。Example 3 During September and October 1989, red rot disease occurred in the threads of Macomb seedlings that were being cultured at a seedling collection center. Seedlings infected with this red rot disease were housed in vats, and one tank (group A) was treated with stabilized chlorine dioxide at a concentration of 50 ppm for 25 hours, and the other tank (group B) was treated with stabilized chlorine dioxide at a concentration of 50 ppm. 80% stabilized chlorine dioxide
ppm and treated for 24 hours.
Ａ群では上記処理後３日後に赤変病症状が現われたが、
Ｂ群では種苗培養後海中栽培に移したが、その間赤変病
の再発は全く認められなかった。In group A, symptoms of red rot disease appeared 3 days after the above treatment, but
In group B, the seedlings were cultured and then transferred to underwater cultivation, but no recurrence of red rot disease was observed during this period.
第１図は赤変病感染マコンブ種苗糸を本発明方法で消毒
処理して栽培して得られるマコンブと赤変病弊感染マコ
ンブの葉長を対比した図であり、第２図は同マコンブの
葉幅を対比した図である。
以上
第１図Figure 1 is a comparison of the leaf lengths of Macelon vulgare obtained by cultivating seedlings of Macelon vulgare infected with Macrophyllum rubella by the method of the present invention and disinfected with the method of the present invention. It is a diagram comparing leaf width. Figure 1 above
Claims (1)
理することを特徴とするマコンブ種苗の赤変病防除方法
。1. A method for controlling red rot of Macombus seedlings, which comprises immersing the Macombus seedlings in a stabilized chlorine dioxide aqueous solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1409990A JP2759538B2 (en) | 1990-01-24 | 1990-01-24 | Control method of red rot disease of makombu seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1409990A JP2759538B2 (en) | 1990-01-24 | 1990-01-24 | Control method of red rot disease of makombu seedling |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03219815A true JPH03219815A (en) | 1991-09-27 |
| JP2759538B2 JP2759538B2 (en) | 1998-05-28 |
Family
ID=11851671
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1409990A Expired - Fee Related JP2759538B2 (en) | 1990-01-24 | 1990-01-24 | Control method of red rot disease of makombu seedling |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2759538B2 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008502723A (en) * | 2004-06-09 | 2008-01-31 | アラーガン、インコーポレイテッド | A stabilized composition comprising a therapeutically active agent, citric acid or a conjugate base, and chlorine dioxide |
-
1990
- 1990-01-24 JP JP1409990A patent/JP2759538B2/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008502723A (en) * | 2004-06-09 | 2008-01-31 | アラーガン、インコーポレイテッド | A stabilized composition comprising a therapeutically active agent, citric acid or a conjugate base, and chlorine dioxide |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2759538B2 (en) | 1998-05-28 |
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