JPH03200730A - Remedy for trichophytosis - Google Patents

Abstract

PURPOSE:To provide the subject remedy for trichophytosis composed of Japanese apricot pulp extraction, aloenin and an aqueous ethanol solution, free from side effects and capable of prolonged administration. CONSTITUTION:A remedy for trichophytosis containing 1.5-4.0 pts.wt., preferably 2.0-3.5 pts.wt. solid matter aloenin obtained by grinding and pressing a mixture composed of 100 pts.wt. 30% aqueous ethanol solution, 10-15 pts.wt., preferably 13-14 pts.wt. Japanese apricot pulp extract and naturally dried mesophyll of Aloe arborescens Mill., carrying out centrifugation and heating the resultant liquid material at <=60 deg.C. Provided that citric acid is contained in an amount of 1.5 4.0 pts.wt. on sodium salt base in 10-15 pts.wt. Japanese apricot pulp extract. To the aloenin solid raw material, the Japanese apricot pulp extract and the aqueous ethanol solution are added and the resultant mixture is stirred using a mixer. The obtained liquid is allowed to stand at a cold and dark place and the supernatant is collected, thus preparing the objective remedy.

Description

[Detailed description of the invention] [Industrial application field] TECHNICAL FIELD The present invention relates to a novel therapeutic agent for white mania.

More specifically, the present invention relates to a therapeutic agent for leukoderma, which comprises plum meat extract, aloenin, and an aqueous ethanol solution.

[Prior art and its problems] It has been known that aloe juice can be used to treat white mania, but its effectiveness is not sufficient, and aloe juice or an ethanol aqueous solution containing it cannot be stored for a long period of time. The problem was that it could not withstand the

[Means for Solving the Problems] The present inventor has conducted intensive research to provide a therapeutic agent for white fungus that has strong bactericidal activity against white fungi and can withstand long-term storage. , completed the invention.

The present invention is a therapeutic agent for leukoderma, which comprises plum meat extract, aloenin, and an aqueous ethanol solution.

Aloenin used in the present invention is a component of Kidachialoe, and is different from aloin (a component of Cape Aloe), which has been known to have an antibacterial effect. Natural drying (25℃, humidity 6)
After crushing the mesophyll of Kidachialoe (0%), it is compressed and centrifuged, and the obtained liquid is heated at 60° C. or lower to obtain a solid, which is used as a raw material for aloenin. Plum meat extract prepared according to a conventional method can be used. That is, it is prepared by crushing fresh plums, squeezing them, centrifuging them, and concentrating the resulting liquid. The plum meat extract thus obtained contains a large amount of citric acid as a sodium salt, and this citric acid is thought to play an important role in the present invention in synergy with aloenin.

There is no particular limit to the concentration of the ethanol aqueous solution, but one containing about 30% ethanol is appropriate;
degree) is preferably used.

The therapeutic agent for white strep throat of the present invention is prepared by adding a plum extract and an aqueous ethanol solution to the above-mentioned solid aloenin raw material, stirring with a mixer, then standing in a cool, dark place, and collecting the supernatant liquid.

As will be shown later, when aloenin is extracted from an aloenin raw material with an aqueous ethanol solution, the presence of plum meat extract increases the extraction efficiency of aloenin.

In the therapeutic agent for white strep throat of the present invention, the ratio of each component is not critical, but 10 to 15 parts by weight (preferably 13 parts by weight) of plum meat extract to 100 parts by weight of 30% ethanol aqueous solution.
~14 parts by weight), 1.5 to 4.0 parts by weight (preferably 2.0 to 3.5 parts by weight) of aloenin, and 1 part by weight of plum meat extract.
0 to 15 parts by weight of citric acid as sodium salt 4.
It is contained in an amount of 0 to 6.0 parts by weight.

When administering the therapeutic agent for white mania of the present invention, 10 ml of liquid solution
150ml of 30% ethanol aqueous solution (preferably shochu)
Add 1 ml of the solution, stir well, and apply 1 ml of the solution to the affected area using a dropper.

Aloenin and plum meat extract, which are the main components of the therapeutic agent of the present invention, are natural products and have no side effects, so they can be used continuously for a long period of time as an anti-white-throngiasis therapeutic agent.

Next, the present invention will be explained in more detail with reference to Examples.

Example 1 32 kg of Kidachialoe mesophyll naturally dried at a temperature of 25° C. and a humidity of 60% was crushed and squeezed, and the result was 2500r, p, +g.
The resulting liquids te and efI were heated at a temperature of 60° C. or lower to obtain a solid. This has 0 plum meat extract.
．． 14N and shochu (35 degrees > 0.8 BI) were added and stirred for 10 minutes with a mixer. It was allowed to stand in a cool and dark place for one week, and the supernatant liquid was collected to obtain the therapeutic agent for leukoderma of the present invention. Thus obtained The therapeutic agent is a red wave body with a sweet alcohol odor and a specific gravity of 0.94-0.98 (g/ml, 20'C).
, pH 4.0-4.2. The composition is shown in Table 1. Furthermore, Table 2 and FIG. 1 show changes in the amount of aloenin extracted depending on the amount of plum meat extract added.

Table 3 shows the results of testing the stability over time of the therapeutic agent for leukoderma of the present invention.

Table 3 Aloenin citric acid (as sodium salt) Evaporation residue (■/ml) 25.2-30.8 44.8-56.0 340-360 As is clear from Table 3, even after two years of storage, There is only a slight decrease in component content and the therapeutic agent of the present invention is extremely stable.

Test Example In VltrO, the anti-white trout activity of this therapeutic agent was investigated.

The test preparations included the present composition, its dilutions (2 types),
Plum extract, aloe juice, shochu as the solvent used, and elot as a standard antifungal agent to confirm the effectiveness of this test.
We selected 7 types of rllaZOle.

Five strains of Trlchophyton were used as test self-binding strains, and the agar medium was
se (2%) agar (SD A), Bac
to-east morphology agar(
YMA) was used. The cells were cultured at 27° C., growth was observed every week, and the lowest drug concentration that completely inhibited growth was defined as the minimum inhibitory concentration (MIC).

The test results are summarized in Table 4.

Looking at the results, first, the MIC value of elotrllaZOlo against the test self-binding bacteria was the same as before, and the white nail fungus used was a strain with standard antibacterial susceptibility.

Next, each test preparation was as follows.

The effect of aloe juice alone was completely dependent on the culture medium, but it suggested that it may have anti-stereotypic activity. Needless to say, shochu, the solvent used, had no antibacterial activity.

Plum extract, which is another active ingredient in this preparation, has been shown to have antibacterial activity, and this preparation, which contains aloe juice and plum extract, contains only 1/7 of the plum extract. Nevertheless, it had the same effect as plum meat extract.

The anti-self-binding activity of this preparation decreased as it was diluted.

As described above, it was confirmed that this preparation has sufficient anti-self-binding activity. Furthermore, since the preparation itself is an alcoholic liquid pharmaceutical composition, it has the characteristic that when applied externally, it can be concentrated by drying to a concentration that provides sufficient antibacterial activity.

Explanation of the analysis method 1) Place the sodium citrate sample in a small glass test tube (φ9.7mm, 7.5am).
Take 50 μg of the solution and evaporate to dryness at 60°C under a nitrogen stream. Add one silylating agent (hexamethyldisilazane, trimethylchlorosilane) to this, and leave it sealed at room temperature.

Regarding the calibration curve standard (special grade sodium citrate),
Prepare an aqueous solution of 0.1.3.5 μ/ml and treat in the same manner as the sample.

Take 5 μQ of the sample solution and inject it into the gas chromatograph operating conditions shown in Table 5 to obtain a chromatogram.

Obtain the analytical value from the calibration curve.

2) Take 50 μg of an aloenin sample into a small glass test tube (described above) and evaporate to dryness at 60° C. under a nitrogen stream. 100 μg of a silylating agent (hexamethyldisilazane, trimethylchlorosilane/pylidene solution) was added to this, and the mixture was left sealed at 60° C. for 1 hour.

For the calibration curve preparation, prepare aqueous solutions of 0, 1, and 3.5 ml/ml and treat in the same manner as the samples.

Collect 5 μQ of the sample solution and inject it into a device with the gas chromatograph operating conditions shown in Table 5 to obtain a chromatogram.

Obtain the analytical value from the calibration curve.

Table 5 Representative chromatograms of these are shown in FIGS. 2 and 3.

Identification was determined from the retention time of each specimen.

Regarding aloenine, there are few impurities around the peak,
It can be seen that the extraction was extremely good. Book G, C
The conditions are that a nonpolar filler is used, and the target is made nonpolar by silylating a polar group such as 10H group, so the components are eluted in order of boiling point. Therefore, if there is a compound close to aloenine, it should show a peak around it. Aloin, the main component of Aloe catarrh, elutes around aloenin. Not detected this time.

For citrate, bistrimethylsilylamide (
4% of the total when using a silylating agent such as BSA)
I just couldn't analyze it. That is, it can be seen that citric acid exists in the present therapeutic agent as some kind of salt.

[Brief explanation of drawings]

FIG. 1 is a graph showing the relationship between plum meat extract concentration and extracted aloenin amount. FIGS. 2 and 3 show a gas chromatograph of the therapeutic agent of the present invention.

Claims (1)

[Scope of Claims] 1) A therapeutic agent for white fungus, which comprises plum meat extract, aloenin, and an ethanol aqueous solution. 2) 10-15 parts by weight of plum meat extract, 1.5-1.5 parts by weight of aloenin
The therapeutic agent for leukoderma according to claim 1, comprising 4.0 parts by weight and 100 parts by weight of a 30% ethanol aqueous solution. 3) The therapeutic agent for white fungus according to claim 2, wherein 10 to 15 parts by weight of plum meat extract contains 2.5 to 6.0 parts by weight of citric acid (as sodium salt).