JPH03117446A - Production of twin bovines using fsh - Google Patents
Production of twin bovines using fshInfo
- Publication number
- JPH03117446A JPH03117446A JP25175789A JP25175789A JPH03117446A JP H03117446 A JPH03117446 A JP H03117446A JP 25175789 A JP25175789 A JP 25175789A JP 25175789 A JP25175789 A JP 25175789A JP H03117446 A JPH03117446 A JP H03117446A
- Authority
- JP
- Japan
- Prior art keywords
- fsh
- rate
- twin
- administered
- production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000283690 Bos taurus Species 0.000 title abstract description 19
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 claims abstract 4
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 claims abstract 4
- 229940028334 follicle stimulating hormone Drugs 0.000 abstract 2
- 206010000210 abortion Diseases 0.000 abstract 1
- 231100000176 abortion Toxicity 0.000 abstract 1
- 238000000034 method Methods 0.000 description 23
- 230000016087 ovulation Effects 0.000 description 16
- 230000000052 comparative effect Effects 0.000 description 13
- 235000013601 eggs Nutrition 0.000 description 12
- 206010000234 Abortion spontaneous Diseases 0.000 description 10
- 208000015994 miscarriage Diseases 0.000 description 10
- 208000000995 spontaneous abortion Diseases 0.000 description 10
- 229940088597 hormone Drugs 0.000 description 9
- 239000005556 hormone Substances 0.000 description 9
- 230000012173 estrus Effects 0.000 description 8
- 230000009027 insemination Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- PXGPLTODNUVGFL-BRIYLRKRSA-N (E,Z)-(1R,2R,3R,5S)-7-(3,5-Dihydroxy-2-((3S)-(3-hydroxy-1-octenyl))cyclopentyl)-5-heptenoic acid Chemical compound CCCCC[C@H](O)C=C[C@H]1[C@H](O)C[C@H](O)[C@@H]1CC=CCCCC(O)=O PXGPLTODNUVGFL-BRIYLRKRSA-N 0.000 description 3
- 241000219198 Brassica Species 0.000 description 3
- 235000003351 Brassica cretica Nutrition 0.000 description 3
- 235000003343 Brassica rupestris Nutrition 0.000 description 3
- 102000009151 Luteinizing Hormone Human genes 0.000 description 3
- 108010073521 Luteinizing Hormone Proteins 0.000 description 3
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000029849 luteinization Effects 0.000 description 3
- 235000010460 mustard Nutrition 0.000 description 3
- 210000001672 ovary Anatomy 0.000 description 3
- 230000035935 pregnancy Effects 0.000 description 3
- PXGPLTODNUVGFL-UHFFFAOYSA-N prostaglandin F2alpha Natural products CCCCCC(O)C=CC1C(O)CC(O)C1CC=CCCCC(O)=O PXGPLTODNUVGFL-UHFFFAOYSA-N 0.000 description 3
- 238000002054 transplantation Methods 0.000 description 3
- 210000004291 uterus Anatomy 0.000 description 3
- 230000007850 degeneration Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 230000000938 luteal effect Effects 0.000 description 2
- 229940040129 luteinizing hormone Drugs 0.000 description 2
- 230000027758 ovulation cycle Effects 0.000 description 2
- 241000283086 Equidae Species 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000034702 Multiple pregnancies Diseases 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 210000004198 anterior pituitary gland Anatomy 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 210000004246 corpus luteum Anatomy 0.000 description 1
- 229960001342 dinoprost Drugs 0.000 description 1
- 230000001158 estrous effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000008217 follicular development Effects 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- PXGPLTODNUVGFL-YNNPMVKQSA-N prostaglandin F2alpha Chemical compound CCCCC[C@H](O)\C=C\[C@H]1[C@H](O)C[C@H](O)[C@@H]1C\C=C/CCCC(O)=O PXGPLTODNUVGFL-YNNPMVKQSA-N 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000004584 weight gain Effects 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Fodder In General (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、Ｆ Ｓ ＩＩによる効率的な牛の双子生産技
術に関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an efficient cow twinning production technique using F S II.
［従来の技術〕
肉用牛生産のためのコストの大部分を占めるのは、繁殖
雌牛の飼養管理費である。したがって、１頭の雌牛から
２頭の辛子を得ることができれば、１頭の辛子を得るよ
りも経済効率は極めて高くなる。そこで、肉資源の経済
的増産の手段として牛の双子生産の技術が検討されてき
た。[Prior Art] Feeding and management costs for breeding cows account for most of the costs for producing beef cattle. Therefore, if two heads of mustard can be obtained from one cow, the economic efficiency will be much higher than that of one head of mustard. Therefore, the technology of cow twin production has been considered as a means of economically increasing the production of meat resources.
従来、牛の双子生産技術には、（１）受精卵２卵移植方
式、（２）追い移植方式（人工授精した後受精卵を移植
）及び（３）ホルモン低単位投与方弐などがある。Conventional cow twin production techniques include (1) a two-fertilized egg transplantation method, (2) a follow-up transplantation method (transplanting a fertilized egg after artificial insemination), and (3) a low-unit hormone administration method.
受精卵２卵移植方式は、後述する方法で採取した受精卵
２個を、借り胎生、すなわち受卵生の子宮に移植して妊
娠させる方法である。受精卵の採取には、能力の高い特
定の雌牛、すなわら供卯牛の黄体期に総量約４０ｍｇ（
又はＡＵ）のＦ Ｓ Ｈを１日２回ずつ４〜５日間に分
割注射することにより卵巣に多数の卵胞を発育させ、Ｆ
ＳＨ初回投与から４８時間ないし７２時間経過後に黄体
退行作用のあるプロスタグランデインＦ２αを注射して
誘起された人工発情時に能力の高い特定の雄牛の精子を
授精させ、約１週間後に子宮を洗浄して受精卵を採取し
、検査、選別を行って良い受精卵、約数個を特殊設備で
保存する。The two-fertilized egg transfer method is a method in which two fertilized eggs collected by the method described below are transplanted into the uterus of a fertilized fetus, that is, a fertilized fetus, to cause pregnancy. To collect fertilized eggs, a total amount of approximately 40 mg (
A large number of follicles are developed in the ovaries by dividing injections of FSH (or AU) twice a day for 4 to 5 days, and F
48 to 72 hours after the first administration of SH, prostaglandin F2α, which has a luteal degeneration effect, is injected to induce artificial estrus, and the sperm of a specific bull with high ability is inseminated, and about a week later, the uterus is washed. The fertilized eggs are collected, inspected and sorted, and a few good fertilized eggs are stored in special equipment.
追い移植方式は、受卵生の自然発情時に人工授精を行い
、約１週間後に、更に別の供卵生から採取した受精卵１
個を移植して双子生産を図る方法である。In the follow-up transplantation method, artificial insemination is performed when the recipient is in natural heat, and approximately one week later, 1 fertilized egg is collected from another donor.
This is a method to produce twins by transplanting individuals.
他方、ホルモン投与方式は、通常１発情時に１個排卵す
る動物である雌牛にホルモンを投与して複数の排卵を誘
起し、多照妊娠させる方法であり、前述の２方法に比し
てホルモン投与以外には特殊処理がなく、手間がかから
すコストも少なくて済む利点がある。On the other hand, the hormone administration method is a method in which hormones are administered to cows, which normally ovulate one egg per estrous, to induce multiple ovulations and produce a multiple pregnancy. Other than that, there is no special processing required, and it has the advantage of requiring less time and effort.
その具体的な方法としては、性周期の黄体期６；＝ある
雌牛に対し、ＰＭＳ（好馬血清性性腺刺激ホルモン）の
注射又はＦＳＨを１日２回ずつ２〜３日間分割注射を行
って卵胞を発育させ、ホルモン初回投与後２４〜４８時
間後に黄体退行を促進させるためプロスタグランデイン
Ｆ２αを注射し、誘起された発情時に１〜２回の授精を
行って受胎・分娩させる方法である。The specific method is to inject PMS (horse serum gonadotropin) or FSH into divided injections twice a day for 2 to 3 days to a cow during the luteal phase 6 of the estrous cycle. This is a method in which prostaglandin F2α is injected to develop follicles and promote corpus luteum regression 24 to 48 hours after the first hormone administration, and one or two inseminations are performed during induced estrus to cause conception and delivery.
（発明が解決しようとする課題〕
しかしながら、移植方式は受精卵のコス１−が高く、そ
の作業には多くの手間を要し、特殊な技術と設備を必要
とするため、現実には実施することが困難な面があった
。(Problem to be solved by the invention) However, in the transplant method, the cost of fertilized eggs is high, the work requires a lot of effort, and requires special technology and equipment, so it is difficult to implement in reality. There were some aspects that were difficult.
また、ホルモン投与方式は、ホルモン投与により１〜８
個の排卵が誘発され、排卵数の増加に比例して受胎率も
向上し、多給率（双子以−トの受胎頭数／受胎頭数）４
０〜８０％の成績が得られたが、３子以上の受胎例では
物理的或いは生理的な原因で妊娠生の子宮は妊娠を継続
できず、流産の発生率が異常に高くなるという問題があ
った。双子の受胎例では流産するものが少ないので、ホ
ルモンの投与方法の調整により排卵数を２個前後に制御
する方法が検討されたが、ホルモン投与による排卵数の
制御は困難でこの方式による双子生産技術は疑問視され
ていた。In addition, the hormone administration method is 1 to 8 depending on the hormone administration.
ovulation is induced, and the pregnancy rate improves in proportion to the increase in the number of ovulations, and the multifeeding rate (number of pregnant cows after twins / number of pregnant cows) 4
The results ranged from 0 to 80%, but in cases of conception of three or more children, the pregnant uterus is unable to continue the pregnancy due to physical or physiological reasons, resulting in an abnormally high incidence of miscarriage. there were. Since few cases of twin conception result in miscarriage, a method of controlling the number of ovulations to around two by adjusting the method of administering hormones was considered, but it was difficult to control the number of ovulations by administering hormones, and this method was not used to produce twins. The technology was questionable.
（課題解決の手段〕
本発明は上記課題を解決することを目的とし、その構成
は、総量６〜１０ｍｇ（又はＡＵ）のＦＳＨを、１日１
回ずつ２日間投与することを特徴とする。(Means for Solving the Problems) The present invention aims to solve the above problems, and its composition is such that a total amount of 6 to 10 mg (or AU) of FSH is administered once a day.
It is characterized by being administered once for two days.
本発明に使用するＦ Ｓ Ｈは家畜、例えば豚、牛、羊
、馬などの下垂体前葉から抽出されるものである。通常
は少量のＬＨ（黄体形成ホルモン）を含有しているが、
本発明におけるＦＳＨ古はＬ Ｈ含有量の多いものから
、精製されＬＨを極く微量にしか含有しないものまです
べてを包含する。F S H used in the present invention is extracted from the anterior pituitary gland of livestock such as pigs, cows, sheep, and horses. Usually contains a small amount of LH (luteinizing hormone),
FSH old in the present invention includes everything from those with a high LH content to those that have been purified and contain only a trace amount of LH.
ホルモンの投与方法は、性周期の黄体期にある雌牛に対
しＦ Ｓ Ｈを１日１回ずつ２日注射して卵巣の卵胞発
育を促進させる。同一時間、すなわら２４時間後に投与
することが好ましいが多少のずれは差支えない。通常Ｆ
ＳＨの第１回投与の２４時間後に、黄体退行因子のプロ
スタグランデインＦ２αを注射し、発情が誘起されたら
１〜２回の授精を行う。Hormone administration involves injecting FSH once a day for two days to cows in the luteal phase of their estrous cycle to promote follicular development in the ovaries. It is preferable that the doses be administered at the same time, ie, 24 hours later, but a slight difference in administration is acceptable. Normal F
Twenty-four hours after the first administration of SH, prostaglandin F2α, a luteal degeneration factor, is injected, and once estrus is induced, one or two inseminations are performed.
ＦＳＨＯ力価は、Ｆ Ｓ Ｈアーマ−標準品を用いてラ
ット卵巣重量増加法によって測定され、ｍｇ又はＡＵで
表わされる。FSHO titer was determined by the rat ovary weight gain method using FSH Armor standards and is expressed in mg or AU.
ＦＳＨの総投与量は６〜１０ｍｇ（又はＡＵ）である。The total dose of FSH is 6-10 mg (or AU).
６ｍｇ （又はＡＵ）未満では２個以−ト排卵する割合
が少なく、したがって双子率が低いので好ましくない、
１０ｍｇ（又はＡＵ）を越えると、発情率、受胎率及び
双子率は高くなるが、３〜５個排卵する割合が高くなり
、流産発生率が高くなるので好ましくない。If it is less than 6 mg (or AU), the rate of ovulation of two or more eggs is low, and therefore the twin rate is low, so it is not preferable.
If it exceeds 10 mg (or AU), the estrus rate, conception rate, and twin rate will increase, but the rate of ovulation of 3 to 5 eggs will increase, and the miscarriage rate will increase, which is not preferable.
ＦＳＨは総投与量６〜１０ｒｎｇ（又はＡ Ｕ ）を２
日に分けて投与する。ＦＳＨ６〜１０ｍｇ（又はＡＵ）
の１回投与では排卵数が増加せず好ましくない。３回以
上投与してもその手間にふされしい効果がなく、投与回
数の増加は省力化の点で好ましくない。FSH is administered at a total dose of 6-10 rng (or A U ) of 2
Administer in divided doses. FSH6-10mg (or AU)
A single administration of is not preferable as it does not increase the number of ovulations. Even if the drug is administered three or more times, the effect is not worth the effort, and increasing the number of doses is not preferable from the point of view of saving labor.
本発明は、雌牛の多給による流産を避ＬＪながら可及的
に多くの双子を生産させるものである。そのためにはＦ
ＳＨの投与量を総量６〜１０ｍｇ（又はＡＵ）とし、２
日に分けて投与することにより、平均排卵数を１．４〜
１．６程度に調整し双子率を２０〜３０％に調整するも
のである。The present invention aims to produce as many twins as possible while avoiding miscarriage due to excessive feeding of cows. For that purpose F
The total dose of SH was 6 to 10 mg (or AU), and 2
By dividing the administration into daily doses, the average number of ovulations can be reduced to 1.4~
The twin rate is adjusted to about 1.6 and the twin rate is adjusted to 20-30%.
以下、実施例により本発明を具体的に説明する。 Hereinafter, the present invention will be specifically explained with reference to Examples.
ＩＵ井二上
繁殖用雌牛７３頭に対し、黄体期に６〜１０ｍｇ（又は
ＡＵ）のＦＳＨ（デンカ製薬味製、商品名アントリン）
を２日に分けて２４時間間隔で注射投与した。初日には
４〜６ｍｇ （又はＡＵ）を投与した。第１回投与の２
４時間後の第２回投与の時にプロスタグランデインＦ２
α−Ａ（クーパーズアニマルヘルス９＠製、商品名エス
トラメイト）を５００μｇ投与した。発情が誘起された
時１〜２回の授精を行った。その結果双子率は２３％、
流産率は３％、辛子率（産子数／分娩頭数）１２６％で
あった。これらの条件及び結果を第１表に記載する。6 to 10 mg (or AU) of FSH (manufactured by Denka Pharmaceutical Flavor, trade name Antolin) during the luteal phase to 73 IU Inagami breeding cows.
was administered by injection at 24-hour intervals over two days. 4-6 mg (or AU) was administered on the first day. 2 of the first dose
Prostaglandin F2 at the second dose 4 hours later
500 μg of α-A (manufactured by Coopers Animal Health 9@, trade name: Estramate) was administered. One or two inseminations were performed when estrus was induced. As a result, the twin rate was 23%.
The miscarriage rate was 3%, and the mustard rate (number of offspring/number of calves) was 126%. These conditions and results are listed in Table 1.
、Ｌ較拠二Ｊ方記支且較朋二立
繁殖用雌牛５７頭を用い、ＦＳＨの投与を行わない無処
置の自然分娩の場合を比較例１とした。Comparative Example 1 was a case in which 57 breeding cows were used and natural calving was performed without administration of FSH.
この場合は双子発生は全例に認められなかった。In this case, twin occurrence was not observed in all cases.
総ｆｆ１３〜５ｍｇ（又はＡ Ｕ ）のＦＳＨを実施例
１と同様に投与した場合を比較例２とし、総量１１〜１
５ｍｇ（又はＡＵ）のＦ Ｓ Ｈを実施例１と同様に投
与した場合を比較例３とした。比較例２の場合には、排
卵数は平均１．１個で双子の発生は全例で認められなか
った。比較例３の場合は、発情率、受胎率が高く排卵数
は平均２．３個で双子率も４０％と高かったが、３〜５
個排卵する例も多く流産発生率は２９％と異常に高がっ
た。以上の結果を第１表に併記した。Comparative Example 2 is a case where a total ff of 13 to 5 mg (or A U ) of FSH was administered in the same manner as in Example 1, and the total amount was 11 to 1.
Comparative Example 3 was a case in which 5 mg (or AU) of F S H was administered in the same manner as in Example 1. In the case of Comparative Example 2, the average number of ovulations was 1.1, and no twins were observed in any case. In the case of Comparative Example 3, the estrus rate and conception rate were high, the number of ovulations was 2.3 on average, and the twin rate was high at 40%, but 3 to 5
There were many cases of individual ovulation, and the miscarriage rate was abnormally high at 29%. The above results are also listed in Table 1.
ＦＳＨ３〜５ｍｇ（又はＡＵ）を１２時間間隔で４回投
与した場合を比較例４とし、ＦＳＨ６〜１０ｍｇ（又は
ＡＵ）を１２時間間隔で４〜６回投与した場合を比較例
５とし、ＦＳＨＩＩ〜１５ｍｇ（又はＡ Ｕ ）を１２
時間間隔で４〜６回投与した場合を比較例６とした。比
較例４では排卵数は１．２個で双子の発生は全例共認め
られながった。Comparative Example 4 is a case in which 3-5 mg (or AU) of FSH is administered 4 times at 12-hour intervals, Comparative Example 5 is a case in which 6-10 mg (or AU) of FSH is administered 4-6 times at 12-hour intervals, and FSHII- 15 mg (or A U ) for 12
Comparative Example 6 was a case in which the drug was administered 4 to 6 times at time intervals. In Comparative Example 4, the number of ovulations was 1.2, and no twins were observed in any case.
比較例５では発情発現も良好で排卵数は平均１．６個で
、双子率は２３％であり、流産発生率は４％と低かった
。比較例６では、発情率、受胎率共に高く、排卵数は平
均２．５と多くなった結果、双子率は４０％と向上した
が、３〜５個排卵する例も多く、流産発生率は４４％と
異常に高かった。以上の結果を第１表に併記した。In Comparative Example 5, the onset of estrus was good, the average number of ovulations was 1.6, the twin rate was 23%, and the miscarriage rate was as low as 4%. In Comparative Example 6, both the estrus rate and conception rate were high, and the number of ovulations increased to an average of 2.5, resulting in an improved twin rate of 40%, but there were many cases of 3 to 5 ovulations, and the miscarriage rate was low. It was an abnormally high 44%. The above results are also listed in Table 1.
（以下余白）
総量として６〜１０ｍｇ（又はＡＵ）のＦＳＨを２日収
−１−にわたって投与する実施例１と比較例５を用いる
と牛の双子生産を流産率を低く行うことができるが、両
者を比較すると比較例５の方法は獣医師の往診回数と注
射回数が多く煩雑であり、そのためのコストも高くなる
ので、実施例１の方法が省力的かつ実用的である。(Margin below) Using Example 1 and Comparative Example 5, in which a total amount of 6 to 10 mg (or AU) of FSH is administered over two days - 1 -, cow twin production can be performed with a low miscarriage rate, but Comparing the two, the method of Comparative Example 5 is complicated and requires a large number of veterinarian visits and injections, which also increases the cost, so the method of Example 1 is labor-saving and practical.
［発明の効果〕
本発明は、特殊な設備及び技術を要することなく、Ｆ
Ｓ Ｈのより少ない投与量、及び投与回数により投与の
コスト及び手間を省略し、流産率を低下させ、双子率を
向上し、産業上きわめて有利な方法である。[Effects of the invention] The present invention enables F
This is an industrially very advantageous method, as it reduces the administration cost and labor by reducing the dose of S H and the frequency of administration, reduces the miscarriage rate, and improves the twin rate.
Claims (1)
モンＦｏｌｌｉｃｌｅＳｔｉｍｕｌａｔｉｎｇＨｏｒｍ
ｏｎｅ）を、１日１回ずつ２日間投与することを特徴と
する牛の双子生産方法。A total of 6-10 mg (or AU) of FSH (Follicle Stimulating Hormone)
1) is administered once a day for 2 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25175789A JPH03117446A (en) | 1989-09-29 | 1989-09-29 | Production of twin bovines using fsh |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25175789A JPH03117446A (en) | 1989-09-29 | 1989-09-29 | Production of twin bovines using fsh |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03117446A true JPH03117446A (en) | 1991-05-20 |
| JPH056982B2 JPH056982B2 (en) | 1993-01-27 |
Family
ID=17227472
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25175789A Granted JPH03117446A (en) | 1989-09-29 | 1989-09-29 | Production of twin bovines using fsh |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03117446A (en) |
-
1989
- 1989-09-29 JP JP25175789A patent/JPH03117446A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH056982B2 (en) | 1993-01-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |