JPH03112495A - Detection of microorganism floating in air - Google Patents
Detection of microorganism floating in airInfo
- Publication number
- JPH03112495A JPH03112495A JP25068889A JP25068889A JPH03112495A JP H03112495 A JPH03112495 A JP H03112495A JP 25068889 A JP25068889 A JP 25068889A JP 25068889 A JP25068889 A JP 25068889A JP H03112495 A JPH03112495 A JP H03112495A
- Authority
- JP
- Japan
- Prior art keywords
- amount
- filter
- air
- microorganisms
- atp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 40
- 238000007667 floating Methods 0.000 title claims abstract description 8
- 238000001514 detection method Methods 0.000 title claims description 4
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 241000233866 Fungi Species 0.000 claims abstract description 21
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 9
- 239000010452 phosphate Substances 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000011148 porous material Substances 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 17
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 1
- 229960005305 adenosine Drugs 0.000 claims 1
- 239000003570 air Substances 0.000 description 29
- 239000002609 medium Substances 0.000 description 9
- 238000007796 conventional method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 235000013305 food Nutrition 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000004065 semiconductor Substances 0.000 description 4
- 102100022936 ATPase inhibitor, mitochondrial Human genes 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 3
- 101000902767 Homo sapiens ATPase inhibitor, mitochondrial Proteins 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 2
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 2
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 2
- 108060001084 Luciferase Proteins 0.000 description 2
- 239000005089 Luciferase Substances 0.000 description 2
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 208000022362 bacterial infectious disease Diseases 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 230000029918 bioluminescence Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
〈産業上の利用分野〉
本発明は食品、飲料、医薬品等の製造またはこれらの充
填、包装に係わる工場、半導体製造工場、あるいは厨房
施設、病院等における環境空気中に浮遊している微生物
を、微生物由来のアデノシン−３−リン酸（以下単にＡ
ＴＰと略記する）の計測に基づいて検出する方法に関す
るものである。[Detailed Description of the Invention] <Industrial Application Field> The present invention is applicable to factories involved in the production of foods, beverages, pharmaceuticals, etc. or their filling and packaging, semiconductor manufacturing factories, kitchen facilities, hospitals, etc. Floating microorganisms are treated with microorganism-derived adenosine-3-phosphate (hereinafter simply A).
The present invention relates to a detection method based on measurement of TP (abbreviated as TP).
〈従来の技術〉
前記工場や施設、あるいは病院等においては、製品に対
する雑菌汚染や患者に対する細菌感染を防止する目的か
ら、工場内やクリーンルーム内、あるいは手術室、集中
治療室内等の室内の空気を常に清浄に保つことが必要で
あり、そのため、これらの環境空気中に浮遊している微
生物の量を常に把握することが重要な業務となっている
。<Conventional Technology> In the factories, facilities, hospitals, etc., the air inside the factories, clean rooms, operating rooms, intensive care units, etc. is controlled to prevent bacterial contamination of products and bacterial infection of patients. It is necessary to keep the environment clean at all times, so it is important to constantly monitor the amount of microorganisms floating in the air.
空気中に浮遊している微生物としては、真菌（主に黴）
および細菌が代表的であり、このような微生物量の把握
には、従来から種々の分析方法が用いられている。Microorganisms floating in the air include fungi (mainly mold)
and bacteria are typical examples, and various analytical methods have been used to determine the amount of microorganisms.
たとえば、寒天培地を拡げたシャーレを、測定すべき環
境空気中に所定時間放置して培地上に落下する微生物を
捕集した後（落下法）、あるいは検体空気をポンプによ
って吸引するとともに吸引した空気をシャーレ上に拡げ
た寒天培地上に吹き付けることによって検体空気中の微
生物を付着、捕集した後（吸引法）、培地を３５℃にて
２４〜７２時間培養し、その後培地上に形成されたコロ
ニーを、たとえばその大きさによって真菌と細菌とに区
別するとともにそれぞれの個数を計数するという方法が
採用されている。For example, after leaving a Petri dish with an agar medium spread out in the ambient air to be measured for a predetermined period of time and collecting microorganisms that fall onto the medium (dropping method), or after sucking the sample air with a pump and using the sucked air. After attaching and collecting microorganisms in the sample air by spraying them onto an agar medium spread on a petri dish (suction method), the medium was cultured at 35°C for 24 to 72 hours, and then the microorganisms formed on the medium were A method has been adopted in which colonies are classified into fungi and bacteria based on their size, and the number of each colony is counted.
また、最近では検体空気を孔径０．４５μｍフィルター
を用いて濾過して当該フィルター上に微生物を捕捉した
後、当該フィルターをフィルターごと培地上で培養し、
その後上述のようにして真菌と細菌の個数を計数すると
いった簡便法も採用されている。In addition, recently, sample air is filtered using a 0.45 μm pore size filter to capture microorganisms on the filter, and then the filter is cultured on a medium together with the filter.
A simple method has also been adopted in which the number of fungi and bacteria is then counted as described above.
〈発明が解決しようとする問題点〉
しかしながら、上述のような従来の空気中浮遊微生物の
検出方法は、いずれも培地を用いて微生物の成育を行い
、その後培地上のコロニー数を計数するという方法であ
るから、培地プレートの調整やコロニー数の目視による
計数といった極めて専門的かつ煩雑な操作が必要であり
、しかも結果が判明するのは２４時間以上も後になると
いう欠点があった。<Problems to be solved by the invention> However, the conventional methods for detecting airborne microorganisms as described above all involve growing microorganisms using a medium and then counting the number of colonies on the medium. Therefore, extremely specialized and complicated operations such as adjusting the culture medium plate and visually counting the number of colonies are required, and the results are not known until 24 hours or more later.
一方、食品、飲料、医薬品、半導体製品等の製造工場、
あるいは病院等においては、その環境空気中に真菌、細
菌等の微生物がどの程度存在するかをいち早く知ること
は、製品の安全性や歩留まり向上の面で、あるいは細菌
感染防止の面で極めて重要なことであり、以前から簡単
かつ迅速な空気中浮遊微生物の検出法の出現が望まれて
いた。On the other hand, manufacturing factories for food, beverages, pharmaceuticals, semiconductor products, etc.
In hospitals, etc., it is extremely important to know as early as possible the extent to which microorganisms such as fungi and bacteria are present in the ambient air, in order to improve product safety and yield, and to prevent bacterial infections. Therefore, there has long been a desire for a simple and rapid method for detecting airborne microorganisms.
本発明はかかる事情に鑑みてなされたものであり、上記
従来方法における操作の煩雑さを省略し、しかも微生物
がコロニーを形成するまでに要していた２４〜７２時間
という時間を極端に短縮する空気中浮遊微生物の検出法
を提供することを目的とするものである。The present invention has been made in view of the above circumstances, and eliminates the complexity of operations in the conventional method described above, and furthermore, extremely shortens the time of 24 to 72 hours required for microorganisms to form a colony. The purpose is to provide a method for detecting airborne microorganisms.
〈問題点を解決するための手段〉
本発明は、空気中に浮遊している微生物を検出するにあ
たり、検体空気を孔径２μｍ以下のフィルターを用いて
濾過すること番こよって当該空気中に存在する微生物を
フィルター上に捕捉した後、捕捉した微生物中のアデノ
シン−３−リン酸の量を計測することを特徴とする空気
中浮遊微生物の検出法である。<Means for solving the problem> The present invention, when detecting microorganisms floating in the air, filters sample air using a filter with a pore size of 2 μm or less, thereby detecting microorganisms present in the air. This is a method for detecting airborne microorganisms, which is characterized by capturing microorganisms on a filter and then measuring the amount of adenosine-3-phosphate in the captured microorganisms.
く作用〉
本発明の空気中浮遊微生物の検出法は、検体空気中に存
在する真菌や細菌等の微生物を孔径２μｍ以下のフィル
ターを用いて濾過、捕捉し、この捕捉した微生物の細胞
膜を、酸素、アニオン系界面活性剤等の溶菌剤を用いて
溶解して細胞中のＡＴＰを抽出し、更に抽出したＡＴＰ
の量を生物発光現象を利用して定量し、当該ＡＴＰ量に
基づいて検体空気中に存在する微生物量を検出するもの
である。Effect> The method for detecting airborne microorganisms of the present invention involves filtering and capturing microorganisms such as fungi and bacteria present in the sample air using a filter with a pore size of 2 μm or less, and removing the cell membranes of the captured microorganisms from oxygen. , ATP in the cells is extracted by dissolving it using a lytic agent such as an anionic surfactant, and the extracted ATP is further extracted.
The amount of ATP is quantified using the bioluminescence phenomenon, and the amount of microorganisms present in the sample air is detected based on the amount of ATP.
上記ＡＴＰＩの定量方法は、発光素であるルシフェリン
の存在下、発光酵素であるルシフェラーゼを用いて、そ
の発光量を螢光光度計（光電子検出管）にて電気的に検
出し、これをＡＴＰ量に換算することによって行う。The above method for quantifying ATPI uses luciferase, which is a luminescent enzyme, in the presence of luciferin, which is a luminescent substance, and electrically detects the amount of luminescence using a fluorophotometer (photoelectron detection tube). This is done by converting to
また、本発明法によれば濾過に使用するフィルターの孔
径を適宜選択することにより、真菌と細菌を区別して検
出することも可能であり、具体的には孔径２μｍのフィ
ルターを用いて計測したＡＴＰ量を真菌由来のＡＴＰ量
とし、また孔径０．４５μｍ以下のフィルターを用いて
計測したＡＴＰ量と孔径２μｍのフィルターを用いて計
測したＡＴＰ量との差を、細菌由来のＡＴＰ量とするも
のである。Furthermore, according to the method of the present invention, by appropriately selecting the pore size of the filter used for filtration, it is possible to distinguish between fungi and bacteria. Specifically, ATP measured using a filter with a pore size of 2 μm The amount of ATP derived from fungi is defined as the amount of ATP derived from bacteria, and the difference between the amount of ATP measured using a filter with a pore size of 0.45 μm or less and the amount of ATP measured using a filter with a pore size of 2 μm is defined as the amount of ATP derived from bacteria. be.
〈実施例〉 以下に実施例をもって本発明の詳細な説明する。<Example> The present invention will be described in detail below with reference to Examples.
去詣■上
ＮＡＳＡ （米国航空宇宙局）規格でクラス１０，００
０のクリーンルーム、および一般の化学実験室において
、孔径０．４μｍのフィルターと孔径２μｍのフィルタ
ーのそれぞれを用いて室内の空気（検体空気）１００ｆ
を吸引濾過し、空気中の微生物を捕捉した。次に、これ
らのフィルターをＡＴＰ測定用の小試験管にとり、ｔＭ
ｓｃｏ−Ｍｉｃｒｏｓｕｒｅ−１００にセットし、溶菌
酵素液を添加した。数分後、ここにルシフェリン、ルシ
フェラーゼを添加し、螢光発光量（ＡＴＰ量）を計測し
た。計測結果を第１表に示す。Class 10,00 according to NASA (National Aeronautics and Space Administration) standards
In a 0.0 clean room and a general chemical laboratory, 100 f of indoor air (sample air) was collected using filters with a pore size of 0.4 μm and a filter with a pore size of 2 μm.
was suction-filtered to capture microorganisms in the air. Next, these filters are placed in a small test tube for ATP measurement, and tM
It was set in sco-Microsure-100, and a lytic enzyme solution was added. After several minutes, luciferin and luciferase were added thereto, and the amount of fluorescence (ATP amount) was measured. The measurement results are shown in Table 1.
また、孔径０．４μｍのフィルターを用いて濾過した場
合のＡＴＰ量（Ａとする。但し、単位は×１０−１０μ
ｇ）と、孔径２μｍのフィルターを用いて濾過した場合
のＡＴＰ量（Ｂとする。同じく単位はｘ ｌ Ｑ−１１
１８ｇ）とから、次式によって検体空気１００１中の真
菌の個数および細菌の個数を計算した。なお、計算に際
しては、細菌１個あたりのＡＴＰ量として１．２Ｘｔｏ
−Ｉ０μｇＡＴＰ／ ｃ ｅ １１の値を用い、真菌１
個あたりのＡＴＰ量として、６０Ｘ１０−１０＃ｇＡＴ
Ｐ／ｃｅｌｌの値を用いた。In addition, the amount of ATP (A) when filtered using a filter with a pore size of 0.4 μm.However, the unit is ×10-10 μm.
g) and the amount of ATP when filtered using a filter with a pore size of 2 μm (denoted as B. Similarly, the unit is x l Q-11
18g), the number of fungi and bacteria in the sample air 1001 was calculated using the following formula. In addition, when calculating, the amount of ATP per bacterium is 1.2Xto
Using a value of −I0 μg ATP/ce 11, fungal 1
As the ATP amount per piece, 60X10-10#gAT
The value of P/cell was used.
細菌数＝ （Ａ−Ｂ）／１．２・・・・・■真菌数＝Ｂ
／６０ ・・・・・■結果を第１表に示す。Number of bacteria = (A-B)/1.2...■ Number of fungi = B
/60...■The results are shown in Table 1.
比較例として、検体空気１ｏｏｚを孔径０．４５μｍの
メンブレンフィルターを用いて濾過し、次いで当該フィ
ルターをフィルターごと寒天培地上に乗せて３５℃にて
４８時間培養し、培養後のコロニーを計数する従来法（
簡便法）を実施した。As a comparative example, the conventional method involves filtering 1 oz of sample air using a membrane filter with a pore size of 0.45 μm, then placing the filter together with the filter on an agar medium, culturing it at 35°C for 48 hours, and counting the colonies after culturing. Law (
A simple method) was carried out.
結果を同じく第１表に示す。The results are also shown in Table 1.
第１表 具ｌ１ｌｌ！１ＩＩＩＢ／１１ｉ。Table 1 Ingredients l1ll! 1IIIB/11i.
去ｌｌ」ｌ
一般の化学実験室において、濾過空気量を５０［１０（
１、および２００１と変えた場合のＡＴＰ量、細菌数、
および真菌数の変化を、実施例１の場合と同様にして調
べた。ＡＴＰ量、細菌数、真菌数の測定結果を第２表に
、また濾過空気量（１）とＡＴＰ検出量との関係を添付
図面に示す。In a general chemistry laboratory, the amount of filtered air is set to 50 [10 (
1, and the amount of ATP when changing from 2001, the number of bacteria,
And changes in the number of fungi were examined in the same manner as in Example 1. The measurement results of the amount of ATP, the number of bacteria, and the number of fungi are shown in Table 2, and the relationship between the amount of filtered air (1) and the detected amount of ATP is shown in the attached drawing.
比較例として、前記簡便法によっても同様な試験を行い
、その結果を第２表に示す。As a comparative example, a similar test was conducted using the above-mentioned simple method, and the results are shown in Table 2.
上記各実施例に示したように、空気中浮遊微生物のフィ
ルターによる濾過捕捉に際しては、濾過に使用するフィ
ルターの孔径によって捕捉し得る微生物の種類が異なり
、孔径０．４５μｍ以下のフィルターによる濾過では細
菌および真菌の両方を捕捉したこととなり、また孔径２
μｍのフィルターによる濾過では、細菌は捕捉されずほ
ぼ真菌のみを捕捉したこととなることがわかる。従って
、前記工場や病院等における環境管理に際して、空気中
に存在する真菌の量だけを把握すればよい場合（たとえ
ば、食品産業分野においてはこのような管理を行うこと
がある）は、孔径２μｍのフィルターを用いて計測した
ＡＴＰ量を管理指標として用いればよく、真菌および細
菌の両方を把握する必要がある場合は、孔径０．４５μ
ｍ以下のフィルターを用いて計測したＡＴＰ量、あるい
は当該ＡＴＰ量と孔径２μｍのフィルターを用いて計測
したＡＴＰＩとの両方を管理指標として用いればよい。As shown in the above examples, when airborne microorganisms are captured by a filter, the types of microorganisms that can be captured differ depending on the pore size of the filter used for filtration. This means that both the pore size and the fungus were captured, and the pore size was 2.
It can be seen that filtration with a μm filter did not capture bacteria but only captured fungi. Therefore, when controlling the environment in factories, hospitals, etc., if it is only necessary to determine the amount of fungi present in the air (for example, this type of control is sometimes carried out in the food industry), a pore size of 2 μm is recommended. The amount of ATP measured using a filter can be used as a control index, and if it is necessary to monitor both fungi and bacteria, a pore size of 0.45μ can be used.
The amount of ATP measured using a filter with a diameter of 2 μm or less, or both the amount of ATP and the ATPI measured using a filter with a pore size of 2 μm may be used as a management index.
また、本発明方法によれば、孔径２μｍのフィルターを
用いて検体空気を濾過した場合のＡＴＰ量を基にして、
前記０式によって真菌の個数を求めることができ、孔径
０．４５μｍ以下のフィルターを用いて濾過した場合の
ＡＴＰＩと、孔径２μｍのフィルターを用いて濾過した
場合のＡＴＰ量とを基にして、前記０式によって細菌の
個数を求めることができ、その結果は上記各実施例に示
したように従来法による測定結果とよく一致している。Furthermore, according to the method of the present invention, based on the amount of ATP when sample air is filtered using a filter with a pore size of 2 μm,
The number of fungi can be determined by the above formula 0, and based on the ATPI when filtered using a filter with a pore size of 0.45 μm or less and the ATP amount when filtered using a filter with a pore size of 2 μm, The number of bacteria can be determined using Equation 0, and the results are in good agreement with the measurement results using the conventional method, as shown in the above examples.
なお、真菌および細菌の両方を捕捉したい場合に用いる
フィルターの孔径は、０．４５μｍ以下であればいくつ
でもよいが、孔径があまり小さいと検体空気を濾過する
際にフィルターの目詰まりが生じて好ましくなく、実用
上は孔径０．２０μｍ以上のものを用いるとよい。Note that when it is desired to capture both fungi and bacteria, the pore size of the filter used may be any size as long as it is 0.45 μm or less, but if the pore size is too small, the filter will become clogged when filtering the sample air, so it is not preferable. For practical purposes, it is preferable to use a material with a pore diameter of 0.20 μm or more.
更に、従来法においては検体空気を濾過した際にフィル
ター上での微生物の重積が生じ易く、よって測定誤差が
大きいため、上記第２表に見られるように濾過空気量と
、検出された細菌数とが比例しないというような現象も
生じるが、本発明法においては微生物重積の影響を全く
受けないので、添付図面に示したごとく濾過空気量とＡ
ＴＰｌｉとはほぼ比例関係にあり、従ってより精度の高
い微生物の検出を行うことができる。Furthermore, in the conventional method, when sample air is filtered, microorganisms tend to accumulate on the filter, resulting in large measurement errors. Although there may be a phenomenon where the number is not proportional to the amount of air, the method of the present invention is not affected by microbial status at all, so the amount of filtered air and A are not proportional to each other as shown in the attached drawing.
There is a substantially proportional relationship with TPli, and therefore microorganisms can be detected with higher accuracy.
く効果〉
本発明によれば、従来法のような菌体の培養や、生菌コ
ロニーの目視による計数といった煩雑かつ専門的な操作
および精度の低い定量、更に培養に２４時間以上もかか
り、定量値を迅速に得られないといった問題点がすべて
解決される。その結果として、食品製造に関わる工場お
よび厨房施設、医薬品工場、半導体工場、病院等におけ
る室内の環境管理は簡便で正確かつ迅速なものとなり、
ひいては食品、医薬品の安全性および半導体製品の歩留
まり向上に貢献し、また病院においては患者に対する衛
生管理をより確実なものとすることができ、よって経済
的にも衛生的にも極めて効果が大きいものである。Effects> According to the present invention, conventional methods require complicated and specialized operations such as culture of bacterial cells and visual counting of viable bacterial colonies, and low precision of quantification, and culture takes more than 24 hours, making quantification difficult. All problems such as not being able to obtain values quickly are solved. As a result, indoor environmental management in food manufacturing factories and kitchen facilities, pharmaceutical factories, semiconductor factories, hospitals, etc. has become simpler, more accurate, and faster.
This will ultimately contribute to improving the safety of food and medicine and the yield of semiconductor products, and in hospitals, it will be possible to ensure better hygiene management for patients, making it extremely effective both economically and hygienically. It is.
添付図面は本発明法による濾過空気量とＡＴＰ測定値と
の関係を示したグラフであり、直線（イ）は孔径２μｍ
のフィルターを用いて検体空気を濾過した場合、直線（
ロ）は孔径０．４μｍのフィルターを用いて検体空気を
濾過した場合である。
濾過空気量
（０The attached drawing is a graph showing the relationship between the amount of air filtered by the method of the present invention and the measured ATP value, and the straight line (A) indicates the pore diameter of 2 μm.
When sample air is filtered using a filter of
B) is the case where the sample air was filtered using a filter with a pore size of 0.4 μm. Filtered air amount (0
Claims (1)
するにあたり、検体空気を孔径２μｍ以下のフィルター
を用いて濾過することによって当該空気中に存在する微
生物をフィルター上に捕捉した後、捕捉した微生物中の
アデノシン−３−リン酸の量を計測することを特徴とす
る空気中浮遊微生物の検出法。 ２、検体空気を孔径２μｍのフィルターを用いて濾過し
た場合に計測されるアデノシン−３−リン酸の量を、真
菌由来のアデノシン−３−リン酸量とする請求項１記載
の空気中浮遊微生物の検出法。 ３、検体空気を孔径０．４５μｍ以下のフィルターを用
いて濾過した場合に計測されるアデノシン−３−リン酸
の量から、検体空気を孔径２μｍのフィルターを用いて
濾過した場合に計測されるアデノシン−３−リン酸の量
を差し引いた値を、細菌由来のアデノシン−３−リン酸
量とする請求項１記載の空気中浮遊微生物の検出法。[Claims] 1. When detecting microorganisms such as fungi and bacteria floating in the air, the microorganisms present in the air are filtered by filtering the sample air using a filter with a pore size of 2 μm or less. 1. A method for detecting airborne microorganisms, which comprises capturing the microorganisms above and then measuring the amount of adenosine-3-phosphate in the captured microorganisms. 2. The airborne microorganism according to claim 1, wherein the amount of adenosine-3-phosphate measured when sample air is filtered using a filter with a pore size of 2 μm is the amount of adenosine-3-phosphate derived from fungi. detection method. 3. From the amount of adenosine-3-phosphate measured when the sample air is filtered using a filter with a pore size of 0.45 μm or less, the amount of adenosine measured when the sample air is filtered using a filter with a pore size of 2 μm 2. The method for detecting airborne microorganisms according to claim 1, wherein the amount of adenosine-3-phosphate derived from bacteria is determined by subtracting the amount of -3-phosphate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25068889A JPH03112495A (en) | 1989-09-28 | 1989-09-28 | Detection of microorganism floating in air |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25068889A JPH03112495A (en) | 1989-09-28 | 1989-09-28 | Detection of microorganism floating in air |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03112495A true JPH03112495A (en) | 1991-05-14 |
Family
ID=17211570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25068889A Pending JPH03112495A (en) | 1989-09-28 | 1989-09-28 | Detection of microorganism floating in air |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03112495A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0789778B1 (en) * | 1994-03-18 | 2001-05-30 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern | Cellular material detection apparatus and method |
| US20120295301A1 (en) * | 2010-01-19 | 2012-11-22 | Noe Miyashita | Floating bacteria trapping device, floating bacteria counting method and floating bacteria counting system |
| JP2015144581A (en) * | 2014-02-03 | 2015-08-13 | 株式会社日立製作所 | Microorganism measuring system and control method for the same |
| CN106662576A (en) * | 2014-07-28 | 2017-05-10 | Lg电子株式会社 | Airborne micro-organism measurement apparatus and measurement method therefor |
-
1989
- 1989-09-28 JP JP25068889A patent/JPH03112495A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0789778B1 (en) * | 1994-03-18 | 2001-05-30 | The Secretary Of State For Defence In Her Britannic Majesty's Government Of The United Kingdom Of Great Britain And Northern | Cellular material detection apparatus and method |
| US20120295301A1 (en) * | 2010-01-19 | 2012-11-22 | Noe Miyashita | Floating bacteria trapping device, floating bacteria counting method and floating bacteria counting system |
| JP2015144581A (en) * | 2014-02-03 | 2015-08-13 | 株式会社日立製作所 | Microorganism measuring system and control method for the same |
| CN106662576A (en) * | 2014-07-28 | 2017-05-10 | Lg电子株式会社 | Airborne micro-organism measurement apparatus and measurement method therefor |
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