JPH0292220A - Potato tuber forming and inducing agent and method for forming and inducing potato tuber - Google Patents
Potato tuber forming and inducing agent and method for forming and inducing potato tuberInfo
- Publication number
- JPH0292220A JPH0292220A JP63242432A JP24243288A JPH0292220A JP H0292220 A JPH0292220 A JP H0292220A JP 63242432 A JP63242432 A JP 63242432A JP 24243288 A JP24243288 A JP 24243288A JP H0292220 A JPH0292220 A JP H0292220A
- Authority
- JP
- Japan
- Prior art keywords
- potato tuber
- jasmonic acid
- inducing
- potato
- shoot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000061456 Solanum tuberosum Species 0.000 title claims abstract description 29
- 235000002595 Solanum tuberosum Nutrition 0.000 title claims abstract description 25
- 230000001939 inductive effect Effects 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims abstract description 12
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical class CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 claims abstract description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 12
- ZNJFBWYDHIGLCU-UHFFFAOYSA-N jasmonic acid Natural products CCC=CCC1C(CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- -1 jasmonic acid compound Chemical class 0.000 claims abstract description 8
- 150000001875 compounds Chemical class 0.000 claims abstract description 7
- 229960005070 ascorbic acid Drugs 0.000 claims abstract description 6
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims abstract description 5
- 235000010323 ascorbic acid Nutrition 0.000 claims abstract description 5
- 239000011668 ascorbic acid Substances 0.000 claims abstract description 5
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229960001669 kinetin Drugs 0.000 claims abstract description 5
- 239000003104 tissue culture media Substances 0.000 claims abstract description 5
- 230000015572 biosynthetic process Effects 0.000 claims description 22
- 239000000411 inducer Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 4
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract description 3
- 239000004062 cytokinin Substances 0.000 abstract description 3
- 239000001963 growth medium Substances 0.000 abstract description 3
- 239000012634 fragment Substances 0.000 abstract description 2
- 125000000217 alkyl group Chemical group 0.000 abstract 1
- 238000005755 formation reaction Methods 0.000 description 16
- 239000002609 medium Substances 0.000 description 11
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000005720 sucrose Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 2
- 235000012015 potatoes Nutrition 0.000 description 2
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 2
- FXRJEFIBUWNJOC-UHFFFAOYSA-N 4-benzyl-1h-benzimidazol-2-amine Chemical compound C=12NC(N)=NC2=CC=CC=1CC1=CC=CC=C1 FXRJEFIBUWNJOC-UHFFFAOYSA-N 0.000 description 1
- GOSWTRUMMSCNCW-HNNGNKQASA-N 9-ribosyl-trans-zeatin Chemical compound C1=NC=2C(NC\C=C(CO)/C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O GOSWTRUMMSCNCW-HNNGNKQASA-N 0.000 description 1
- XXFACTAYGKKOQB-SSDOTTSWSA-N Dihydrozeatin Natural products OC[C@H](C)CCNC1=NC=NC2=C1NC=N2 XXFACTAYGKKOQB-SSDOTTSWSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000002211 L-ascorbic acid Substances 0.000 description 1
- 235000000069 L-ascorbic acid Nutrition 0.000 description 1
- 101100353042 Mycobacterium bovis (strain BCG / Pasteur 1173P2) lnt gene Proteins 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000973887 Takayama Species 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 235000010290 biphenyl Nutrition 0.000 description 1
- 239000004305 biphenyl Substances 0.000 description 1
- 125000006267 biphenyl group Chemical group 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- XXFACTAYGKKOQB-ZETCQYMHSA-N dihydrozeatin Chemical compound OC[C@@H](C)CCNC1=NC=NC2=C1NC=N2 XXFACTAYGKKOQB-ZETCQYMHSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 230000032297 kinesis Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- KPRDQXYAVDJJOC-UHFFFAOYSA-N n-cyclohexyl-7h-purin-2-amine Chemical compound C1CCCCC1NC1=NC=C(NC=N2)C2=N1 KPRDQXYAVDJJOC-UHFFFAOYSA-N 0.000 description 1
- FUWGLNKQNHIDAH-UHFFFAOYSA-N n-phenyl-7h-purin-2-amine Chemical compound N=1C=C2NC=NC2=NC=1NC1=CC=CC=C1 FUWGLNKQNHIDAH-UHFFFAOYSA-N 0.000 description 1
- ZUOUZKKEUPVFJK-UHFFFAOYSA-N phenylbenzene Natural products C1=CC=CC=C1C1=CC=CC=C1 ZUOUZKKEUPVFJK-UHFFFAOYSA-N 0.000 description 1
- 101150028022 ppm1 gene Proteins 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- GOSWTRUMMSCNCW-UHFFFAOYSA-N trans-zeatin riboside Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1OC(CO)C(O)C1O GOSWTRUMMSCNCW-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Abstract
Description
【発明の詳細な説明】
（産業上の利用９腎）
本発明は、馬鈴薯塊茎形成誘導剤及び同形成誘導方法に
関する。特に、組織培養方法を用いて馬鈴菌塊茎形成誘
導する際に有用な馬鈴薯塊茎形成誘導剤及び同形成誘導
方法に関する。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application 9) The present invention relates to an agent for inducing potato tuber formation and a method for inducing tuber formation. In particular, the present invention relates to a potato tuber formation inducing agent and a method for inducing potato tuber formation that are useful when inducing tuber formation using a tissue culture method.
（従来の技術）
近年、馬鈴薯の１ｆｉＳ培養によって得られる塊茎を馬
鈴薯の無菌的急速増殖方法に用いることが注目されてい
る。この方法においては、馬鈴薯植物を組織培養して１
．塊茎を形成誘導する点にポイントがある。(Prior Art) In recent years, attention has been paid to the use of tubers obtained by 1fiS culture of potatoes in a method for aseptic rapid propagation of potatoes. In this method, potato plants are tissue cultured to
．． The key point is to induce tuber formation.
塊茎を形成誘導するのに適する組織培養培地のｆｆｉ戊
が、「園芸学会昭和６２年、秋季大会誌」第２６６．２
２７頁（発表者、歇１）求、高山真情）において、既に
提案されている。FFI, a tissue culture medium suitable for inducing tuber formation, was published in the 1986 Autumn Conference Journal of the Horticultural Society, No. 266.2.
It has already been proposed on page 27 (presenter, 1) Moto, Takayama Masjo).
同刊行物では、まず、ｆｆｌ織培前培養培地るムラシゲ
−スクーグ（Ｍｕｒａｓｉｇｅ−Ｓｋｏｏｇ）培地のシ
ュークロース温度を３％に調整した培地で組織培養して
、無菌シュートを育成（Ｐｈａｓｅ １ ） Ｌ、次に
、同培地のシュークロース濃度を高濃度（９％）に調整
した培地で組織培養（Ｐｈａｓｅ ２ ） Ｌ、て、塊
茎の形成量を増大させたことが報告されている。In the same publication, tissue culture is first performed using a Murashige-Skoog medium (FFL preculture medium) in which the sucrose temperature is adjusted to 3%, and sterile shoots are grown (Phase 1) L. Next, it has been reported that the amount of tubers formed was increased by tissue culture (Phase 2) L in the same medium in which the sucrose concentration was adjusted to a high concentration (9%).
この方法では、Ｐｈａｓｅ １で育成された無菌シュー
トをＰｈａｓｅ ２の培地に移植するか、Ｐｈａｓｅ
２の培地に取り替えることを必要とし、この際、多大の
労力を要する点に課題があった。In this method, sterile shoots grown in Phase 1 are transplanted into Phase 2 medium, or
There was a problem in that it was necessary to replace the medium with No. 2, and this required a great deal of labor.
（発明が解決しようとする課題）
本発明は、従来技術に見られる上記課題を解決するとと
もに、−層有効な馬鈴薯塊茎形成誘導剤及び固剤を用い
た馬鈴薯塊茎形成誘導方法を提供せんとするものである
。(Problems to be Solved by the Invention) The present invention aims to solve the above-mentioned problems seen in the prior art, and also to provide a method for inducing potato tuber formation using an effective potato tuber formation inducing agent and solidifying agent. It is something.
（課題を解決するための手段）及び（作用）本発明は、
アスコルビン酸とジャスモン酸類化合物とを有効成分と
して含有することを特徴とする馬鈴薯塊茎形成誘導剤、
アスコルビン酸とジャスモン酸類化合物とサイトカイニ
ン酸化合物とを有効成分として含有することを特徴とす
る馬鈴薯塊茎形成誘導剤及び前記二剤のいずれかを組織
培養培地中に添加することを特徴とする馬鈴薯塊茎形１
′Ｒ，誘導方法を要旨とするものである。(Means for solving the problem) and (action) The present invention includes:
A potato tuber formation inducer characterized by containing ascorbic acid and a jasmonic acid compound as active ingredients,
A potato tuber formation inducer characterized by containing ascorbic acid, a jasmonic acid compound, and a cytokinic acid compound as active ingredients, and a potato tuber-forming agent characterized by adding one of the two agents to a tissue culture medium. 1
'R, the gist is the guidance method.
本発明に用いられるジャスモン酸類化合物とは、次の一
般式１又は■で表される化合物である。The jasmonic acid compound used in the present invention is a compound represented by the following general formula 1 or (2).
上記ジャスモン酸類化合物は、好ましくは、１２−β−
ｏ−Ｄ−グルコピラノシロキシ−ジャスモン酸、メチル
ジャスモン酸、ジャスモン酸又は６−ヒドロキシ−ジャ
スモン酸である。The above jasmonic acid compound is preferably 12-β-
o-D-glucopyranosiloxy-jasmonic acid, methyljasmonic acid, jasmonic acid or 6-hydroxy-jasmonic acid.
本発明に用いられるサイトカイニン類化合物とは、カイ
ネチン、セニルアミノプリン、フェニルアミノプリン、
ベンジルアミノプリン、シクロへキシルアミノプリン、
０−クロロベンジルアミノプリン、０−メチルベンジル
アミノプリン、ジフェニルｉｓ、４−ピリジルフェニル
尿！、４−ベンジルアミノベンズイミダゾール、６−イ
ツベンテニルアミノプリン、トランス−ゼアチン、シス
−ゼアチン、トランスゼアチンリボシド、トランスゼア
チンモノリボチド、ジヒドロゼアチンなどである。The cytokinin compounds used in the present invention include kinetin, senylaminopurine, phenylaminopurine,
benzylaminopurine, cyclohexylaminopurine,
0-chlorobenzylaminopurine, 0-methylbenzylaminopurine, diphenyl is, 4-pyridylphenyluria! , 4-benzylaminobenzimidazole, 6-itzbentenylaminopurine, trans-zeatin, cis-zeatin, trans-zeatin riboside, trans-zeatin monoribotide, dihydrozeatin, and the like.
馬鈴薯塊茎を形成誘導するためには、まず、馬鈴薯植物
の茎頂点培養・又は発根接法により育成した頂芽又は節
を含む茎断片（以下、これを「切片」という。）を組織
培養培地で約４週間育成して、無菌シュートを得る。次
に、無菌シュートの培地中に、アスコルビンｒ１ｉ１０
０〜５０００ρρｍ１好ましくは５００〜２０００ρρ
ｍとジャスモン酸類化合物０．３〜１２ｐｐｍ。In order to induce the formation of potato tubers, first, stem fragments (hereinafter referred to as "slices") containing apical buds or nodes (hereinafter referred to as "slices") grown by stem apex culture or rooting method of potato plants are placed in a tissue culture medium. The seeds are grown for about 4 weeks to obtain sterile shoots. Next, add ascorbine r1i10 into the medium of the sterile shoots.
0-5000ρρm1 preferably 500-2000ρρ
m and 0.3 to 12 ppm of jasmonic acid compounds.
好ましくは１〜５ρρｍとを添加し、さらに２〜４週間
培養すると無菌シュートの節に塊茎が形成誘導されるの
である。Preferably, by adding 1 to 5 ρρm and culturing for 2 to 4 weeks, tubers are induced to form at the nodes of the sterile shoots.
同様にして、無菌シュートの培地中に、アスコルビンｒ
ｖ１１００〜５０００ｐｐｍ、 Ｈましくは５００〜２
０００ｐｐｍとジャスモン酸類化合物０．３〜＋２Ｐρ
ｍ１好ましくは１〜５ｐｐｍとサイトカイニン類化合物
０．５〜１０ｐｐｍ、好ましくは１〜５ρρｍを添加し
、さらに２〜４週間培着すると無菌シュートの節に塊茎
が形成誘導されるのである。Similarly, ascorbic r
v1100~5000ppm, H preferably 500~2
000ppm and jasmonic acid compounds 0.3~+2Pρ
By adding preferably 1 to 5 ppm of m1 and 0.5 to 10 ppm of a cytokinin compound, preferably 1 to 5 ρρm, and culturing for a further 2 to 4 weeks, tubers are induced to form at the nodes of sterile shoots.
（実施例）
馬鈴薯切片をＭｉ織培養する培地として、第１表に示す
組成を有するリンスマイヤー−スクーグ（ｌｎｓｍａｉ
ｅｒ＆Ｓｋｏｏｇ）培地（以下、ｒＬｓ培地」と略称す
る。）を用いた。(Example) As a medium for culturing potato sections with Mi tissue, Linsmaier-Skoog having the composition shown in Table 1 was used.
er&Skoog) medium (hereinafter abbreviated as "rLs medium") was used.
第１表ＬＳ培地組成（ｎ＋ｑ／Ｌ）
ＭｇＳＯ４−７Ｈ２０３７０ＣａＣＬ、 ・２Ｈ２０４
４０ＫＮＯ３＋、９００ ８Ｈ，ＮＯ３＋、６５０
Ｋｌ−１，Ｐｏ、 １７０ Ｆｅ５０４
−７Ｈ，０・２７．８Ｎａ２ＥＤＴ＾ ３７．３
Ｍｎ５０４−４日、０ ２２．３Ｚｎ５Ｏ，−７
Ｈ２０８，６Ｃｕ５Ｏ，−５Ｈ２００，０２５ＣｏＣＬ
２・６Ｈ２００，０２５ＫＩ Ｏ，
８３）１３９０． ６．２ Ｎａ２Ｍｏ
Ｏ４−５ＨＬ０ ０．２５シユークロース ３０
，０００ ミオイノシトール １
００塩酸チアミシ ０．４
組織培養は、直径２．２ＣＬ高さ１５ｃｍの管ビン中に
ＬＳ培地１０ｍ（を入れ、２０°Ｃ連続明条件で４週間
培養し、平均茎長１２ｃｍの無菌シュートを育成した。Table 1 LS medium composition (n+q/L) MgSO4-7H20370CaCL, ・2H204
40KNO3+, 900 8H,NO3+, 650
Kl-1, Po, 170 Fe504
-7H, 0・27.8Na2EDT^ 37.3
Mn504-4 days, 0 22.3Zn5O,-7
H208,6Cu5O,-5H200,025CoCL
2・6H200,025KI O,
83) 1390. 6.2 Na2Mo
O4-5HL0 0.25 Seuucrose 30
,000 Myo-inositol 1
For tissue culture, 10 m of LS medium was placed in a tube bottle with a diameter of 2.2 CL and a height of 15 cm, and cultured for 4 weeks under continuous light conditions at 20°C to grow sterile shoots with an average stem length of 12 cm. did.
無菌シュートを切断して得た切片を、さらに、同様の条
件で培養を繰り返し、供試無菌シュートを必要数育成し
た。The sections obtained by cutting the sterile shoots were further cultured under the same conditions to grow the required number of test sterile shoots.
このようにして得た無菌シュートの管ビン中に、予め第
２表に示す組成に調整した水溶液者１００ＰＬを添加し
た。さらに、２０°Ｃ連続暗条件に置き、２週間後及び
４週間後に塊茎′の形成数を数えた。100 PL of an aqueous solution whose composition had been adjusted in advance as shown in Table 2 was added to the tube bottle of the sterile shoot thus obtained. Furthermore, the plants were placed under continuous dark conditions at 20°C, and the number of formed tubers was counted after 2 and 4 weeks.
（只］ 余り）
第２表 調整した水ＰｅＩ液の組成（１００ＰＬ中）対
照区は、Ｌ−アスコルビン酸、１２−β−〇−Ｄ−グル
コピラノシロキシ−ジャスモン酸、メチルジャスモン酸
、ジャスモン酸、６−ヒドロキシ−ジャスモン酸及びカ
イネチンをそれぞれ単独に、同様にして添加した単独使
用区並びにシュークロース濃度を９％に調整したＬＳ培
地に、無菌シュートを移植した従来法区とし、組織培養
条件は、いずれも同一とした。(Remainder) Table 2 Composition of prepared water PeI solution (in 100 PL) Control group: L-ascorbic acid, 12-β-〇-D-glucopyranosiloxy-jasmonic acid, methyljasmonic acid, jasmonic acid , 6-hydroxy-jasmonic acid and kinetin were added individually in the same manner, and the conventional method was used in which sterile shoots were transplanted to LS medium with the sucrose concentration adjusted to 9%. The tissue culture conditions were as follows: , all were the same.
その結果を第３表に示す。The results are shown in Table 3.
第３表塊茎の形成数
２週間後 ４週間後
本発明図１ ２．１ ３．２本発明
区２ １．７ ２．６本発明区３
２．０ ２．７木発明区４
２．１ ３．２木発明区５
２．０ ３．１ｆｌｉ独使用区
アスコルし゛渕シ：＋、０００ｐｐｍ
Ｏ０，３１２−ρ−ｏ−Ｄ−り１し］ヒ０ラノシ０＋
シーシ゛ｐスｆ：を酸：３．８８ｐｐｍ
０ ０メｆルシ−ｒスｔシｍ
：２．２４ｐｐｍ ０
０． ２シ）スｔシ酸：２．１０ρρｍ
０ ０６−ＩＪ”ｏ牛シー
シ゛？スＥ：Ｉｆｉｌ：２．１２ｐｐｍ ＯＯカイネ
ヂシ＝２．５ρρｍ ０
０． １従来法区
１．６ ２．３注）１、形成数は、いずれも１
０回反復の平均イ１ｊ。Table 3 Number of tubers formed 2 weeks later 4 weeks later Invention figure 1 2.1 3.2 Invention area 2 1.7 2.6 Invention area 3
2.0 2.7 Tree invention area 4
2.1 3.2 Wood invention area 5
2.0 3.1fli German area Ascol Shibuchi: +, 000ppm
O0,312-ρ-o-D-ri1shi] Hi0ranoshi0+
Sheeps f: Acid: 3.88 ppm
0 0 mel f rst s m
:2.24ppm 0
0. 2) Stoxylic acid: 2.10ρρm
0 06-IJ”o Cow Sheet E: Ifil: 2.12ppm OO Kinesis = 2.5ρρm 0
0. 1 Conventional law district
1.6 2.3 Note) 1. The number of formations is 1.
Average i1j of 0 repetitions.
２、本発明図の培地には、いずれもアスコルビンｉ２＋
ｏｏｏρρｍを含有するとともに、本発明図１には、１
２−β−ｏ−Ｄ−グルコピラノシロキシ−ジャスモン酸
３．８８ｐｐｍ、本発明図２には、メチルジャスモン酸
２．２４ｐｐｍ、本発明図３には、ジャスモン酸２．１
０ｐｐｍ１本発明区４には、６−ヒドロキシ−ジャスモ
ン酸２．１２ｐｐｍ、本発明図５には、１２−β−ｏ−
Ｄ−グルコピラノシロキシ−ジャスモン酸３． ｓｓｐ
ｐｍ及びカイネチン２．５Ｐρｍをも含有する。2. The culture medium of the present invention contains ascorbine i2+
In addition to containing oooρρm, 1
3.88 ppm of 2-β-o-D-glucopyranosiloxy-jasmonic acid, 2.24 ppm of methyl jasmonic acid in Figure 2 of the invention, and 2.1 ppm of jasmonic acid in Figure 3 of the invention.
0 ppm1 Invention area 4 contains 2.12 ppm of 6-hydroxy-jasmonic acid, and invention area 4 contains 12-β-o-
D-glucopyranosiloxy-jasmonic acid 3. ssp
It also contains pm and kinetin 2.5Pρm.
第２表から明らかな通り、本発明図１〜５は、いずれも
２及び４週間後の塊茎形成数で、従来法区を上回り、優
れた塊茎形成能があることを示した。単独使用区は、い
ずれも塊茎をほとんど形成しなかった。As is clear from Table 2, the number of tubers formed by the present invention in FIGS. 1 to 5 after 2 and 4 weeks exceeded that of the conventional method, indicating that they had excellent tuber forming ability. Almost no tubers were formed in the single-use plots.
（効果）
本発明の馬鈴薯塊茎形成誘導剤及び馬鈴薯塊茎形成誘導
方法によって、馬鈴薯植物の組織培養によって、大量の
塊茎を確実に形成誘導することができる。(Effects) By using the potato tuber formation inducing agent and the potato tuber formation inducing method of the present invention, it is possible to reliably induce the formation of a large amount of tubers by tissue culture of potato plants.
特許出願人 日本たばこ産業株式会社Patent applicant: Japan Tobacco Inc.
Claims (5)
成分として含有することを特徴とする馬鈴薯塊茎形成誘
導剤。(1) A potato tuber formation inducer characterized by containing ascorbic acid and a jasmonic acid compound as active ingredients.
コピラノシロキシ−ジャスモン酸、メチルジャスモン酸
、ジャスモン酸又は６−ヒドロキシ−ジャスモン酸であ
る請求項１の馬鈴薯塊茎形成誘導剤。(2) The potato tuber formation inducer according to claim 1, wherein the jasmonic acid compound is 12-β-o-D-glucopyranosiloxy-jasmonic acid, methyl jasmonic acid, jasmonic acid or 6-hydroxy-jasmonic acid.
する請求項１又は２の馬鈴薯塊茎形成誘導剤。(3) The potato tuber formation inducer according to claim 1 or 2, which also contains a cytokinic acid compound as an active ingredient.
項３の馬鈴薯塊茎形成誘導剤。(4) The potato tuber formation inducer according to claim 3, wherein the cytokinic acid compound is kinetin.
薯塊茎形成誘導剤を添加することを特徴とする馬鈴薯塊
茎形成誘導方法。(5) A method for inducing potato tuber formation, which comprises adding the potato tuber formation inducing agent of claim 1, 2, 3, or 4 into a tissue culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63242432A JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63242432A JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0292220A true JPH0292220A (en) | 1990-04-03 |
| JP2598811B2 JP2598811B2 (en) | 1997-04-09 |
Family
ID=17089008
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63242432A Expired - Lifetime JP2598811B2 (en) | 1988-09-29 | 1988-09-29 | Potato tuber formation inducer and method for inducing the same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2598811B2 (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995012311A1 (en) * | 1993-11-03 | 1995-05-11 | The United States Of America, Represented By The Secretary Of Agriculture | Natural suppression of sprouting in stored potatoes using jasmonates |
| NL1001620C2 (en) * | 1995-06-22 | 1996-12-24 | Instituut Voor Agrobiologisch | Improvement in activity of plant growth regulators |
| WO1998037173A3 (en) * | 1997-02-21 | 1998-11-19 | Layla Zakaria Abdelrahman | Sugar cane production |
| WO2004057000A3 (en) * | 2002-12-23 | 2004-10-28 | Florisys Inc | Methods and compositions for modulating tuber formation in tuber-producing plants |
| CN102405831A (en) * | 2011-08-15 | 2012-04-11 | 山东省农业科学院蔬菜研究所 | Solid culture medium for callus seedlings of stem tips of potatoes |
-
1988
- 1988-09-29 JP JP63242432A patent/JP2598811B2/en not_active Expired - Lifetime
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995012311A1 (en) * | 1993-11-03 | 1995-05-11 | The United States Of America, Represented By The Secretary Of Agriculture | Natural suppression of sprouting in stored potatoes using jasmonates |
| US5436226A (en) * | 1993-11-03 | 1995-07-25 | The United States Of America, As Represented By The Secretary Of Agriculture | Natural suppression of sprouting in stored potatoes using jasmonates |
| NL1001620C2 (en) * | 1995-06-22 | 1996-12-24 | Instituut Voor Agrobiologisch | Improvement in activity of plant growth regulators |
| WO1997000614A1 (en) * | 1995-06-22 | 1997-01-09 | Instituut Voor Agrobiologisch En Bodemvruchtbaarheidsonderzoek (Ab-Dlo) | Influencing the activity of plant growth regulators |
| US6242381B1 (en) | 1995-06-22 | 2001-06-05 | Instituut Voor Agrobiologisch En Bodemvruchtbaarheidsonderzoek | Influencing the activity of plant growth regulators |
| WO1998037173A3 (en) * | 1997-02-21 | 1998-11-19 | Layla Zakaria Abdelrahman | Sugar cane production |
| WO2004057000A3 (en) * | 2002-12-23 | 2004-10-28 | Florisys Inc | Methods and compositions for modulating tuber formation in tuber-producing plants |
| CN102405831A (en) * | 2011-08-15 | 2012-04-11 | 山东省农业科学院蔬菜研究所 | Solid culture medium for callus seedlings of stem tips of potatoes |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2598811B2 (en) | 1997-04-09 |
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