JPH0253479A - Culture device - Google Patents
Culture deviceInfo
- Publication number
- JPH0253479A JPH0253479A JP20144988A JP20144988A JPH0253479A JP H0253479 A JPH0253479 A JP H0253479A JP 20144988 A JP20144988 A JP 20144988A JP 20144988 A JP20144988 A JP 20144988A JP H0253479 A JPH0253479 A JP H0253479A
- Authority
- JP
- Japan
- Prior art keywords
- medium
- hollow fiber
- fiber membrane
- culture
- line
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012528 membrane Substances 0.000 claims abstract description 42
- 239000012510 hollow fiber Substances 0.000 claims abstract description 41
- 210000002955 secretory cell Anatomy 0.000 claims abstract description 22
- 238000011084 recovery Methods 0.000 claims abstract description 11
- 239000002609 medium Substances 0.000 claims description 92
- 239000001963 growth medium Substances 0.000 claims description 35
- 230000028327 secretion Effects 0.000 claims description 15
- 238000012258 culturing Methods 0.000 claims description 4
- 239000012466 permeate Substances 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 238000004113 cell culture Methods 0.000 claims 1
- 210000004408 hybridoma Anatomy 0.000 abstract description 26
- 239000012141 concentrate Substances 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000016784 immunoglobulin production Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000004926 polymethyl methacrylate Substances 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000004627 regenerated cellulose Substances 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、分泌性細胞を培養して、該分泌性細胞から産
生じた分泌物を効率よく濃縮するための培養装置に関す
る。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a culture device for culturing secretory cells and efficiently concentrating secretions produced from the secretory cells.
本発明に使用する分泌性細胞とは、モノクロナール抗体
産生のハイブリドーマ、ワクチン産生の繊維芽細胞、サ
イトカイン産生のリンパ球、マクロファージ等であるが
、これらに限定されるものではなく、本発明の使用目的
に合致するものであれば何でも使用することができる。The secretory cells used in the present invention include, but are not limited to, monoclonal antibody-producing hybridomas, vaccine-producing fibroblasts, cytokine-producing lymphocytes, macrophages, etc. You can use anything that meets your purpose.
［従来技術および従来技術の課題］
現在、分泌性細胞の培養容器としてフラスコ、ローラー
ボトル、スピナーフラスコおよびこれらの培養容器は、
分泌性細胞を培地と共に同一容器内で培養し、分泌性細
胞の培養および分泌物の産生な行なうものであった。[Prior art and problems with the prior art] Currently, flasks, roller bottles, spinner flasks, and these culture containers are used as culture containers for secretory cells.
Secretory cells were cultured together with a medium in the same container, and secretory cells were cultured and secreted products were produced.
しかしながら、増殖した分泌性細胞と共に産生じた分泌
物を分離採取する際には、分泌性細胞と共に遠心分離容
器中に移して遠心分離処理を行ない、分泌性細胞と分泌
物を含む上澄みとに分離しなければならない、また産生
ずる分泌物の量も通常微少量なので、純粋な分泌物を精
製するには損失が多く、必ずしも最良の方法ではない。However, when separating and collecting secretions produced together with proliferated secretory cells, the secretory cells are transferred to a centrifugation container and centrifuged to separate the secretory cells and the supernatant containing the secretions. Since the amount of secretion that must be purified and produced is usually very small, purification of pure secretion involves large losses and is not necessarily the best method.
そこで、本発明者らは鋭意検討を重ねた結果、次の発明
に到達した。As a result of extensive studies, the inventors of the present invention have arrived at the following invention.
［課題を解決するための手段］
第一の発明として、
例えば、分泌性細胞の１つであるハイブリドーマを培養
し、該ハイブリドーマから産生されたモノクロナール抗
体を濃縮する培養装置１であって、モノクロナール抗体
を透過する中空糸膜７を本体４内部に装填したハイブリ
ドーマの培養容器２とモノクロナール抗体の透過な阻止
する中空糸膜１４を本体１１内部に装填したそノクロナ
ール抗体の濃縮容器３を、培地供給ライン１８、培地回
収ライン１９、培地排出ライン２０、培地循環ライン２
１からなる培地循環回路の途中に配置した培Ｉ！装置１
を提供するものであり、
第二の発明として、
前記第一の発明の培養装置１であって、途中に培地供給
ポンプＭ１を設けた培地供給ライン１８の上流側に、途
中に培地移送ポンプＭ３を設けた培地移送ライン２３を
介して培地調整タンク２２を連結した培地供給タンク１
７を設け、培地回収ライン１９の途中に設けた培地管理
センサー２４と前記培地移送ポンプＭ、を連動制御する
とともに培地排出ライン２０２０ａの途中に設けた培地
排出ポンプＭ２と前記培地移送ポンプＭ、を連動制御す
る培養装置１提供するものである。[Means for Solving the Problems] As a first invention, there is provided a culture device 1 for culturing hybridomas, which are one of secretory cells, and concentrating monoclonal antibodies produced from the hybridomas, comprising: A hybridoma culture container 2 in which a hollow fiber membrane 7 that permeates the monoclonal antibody is loaded inside the main body 4, and a concentration container 3 for the monoclonal antibody in which the hollow fiber membrane 14 that blocks the permeation of the monoclonal antibody is loaded inside the main body 11, Medium supply line 18, medium recovery line 19, medium discharge line 20, medium circulation line 2
Culture medium I placed in the middle of the culture medium circulation circuit consisting of 1! Device 1
As a second invention, in the culture apparatus 1 of the first invention, a medium transfer pump M3 is provided on the upstream side of the medium supply line 18 in which the medium supply pump M1 is provided. A culture medium supply tank 1 connected to a culture medium adjustment tank 22 via a culture medium transfer line 23 provided with
7 is provided to interlock control the medium management sensor 24 provided in the middle of the medium recovery line 19 and the medium transfer pump M, and also control the medium discharge pump M2 and the medium transfer pump M provided in the middle of the medium discharge line 2020a. A culture device 1 that is controlled in an interlocking manner is provided.
［作用］
ハイブリドーマの培養容器２内の中空糸膜７表面では、
ハイブリドーマが増殖すると共にモノクロナール抗体が
産生ずる。[Function] On the surface of the hollow fiber membrane 7 in the hybridoma culture container 2,
As hybridomas proliferate, monoclonal antibodies are produced.
産生じたモノクロナール抗体は、中空糸膜７を透過し、
中空糸１ｉ７の内部を流れる培地と共に合流し、培地導
出口ｌＯ１培地回収ライン１９、分泌物の濃縮器３の培
地導入０１６を経て、中空糸膜１４内に導入される。The produced monoclonal antibody passes through the hollow fiber membrane 7,
It merges with the medium flowing inside the hollow fiber 1i7, and is introduced into the hollow fiber membrane 14 through the medium outlet lO1 medium recovery line 19 and the medium introduction 016 of the secretion concentrator 3.
分泌物は中空糸膜１４を透過できず、中空糸膜１４内に
濃縮される。Secretions cannot pass through the hollow fiber membrane 14 and are concentrated within the hollow fiber membrane 14.
このようにして、増殖した分泌性細胞と分泌性細胞によ
り産生じた分泌物は完全に分離？！ＩＡｔａして採集す
ることができる。In this way, the proliferated secretory cells and the secretions produced by the secretory cells are completely separated? ! It can be collected as IAta.
［実施１！１ｌ１１
第１図は、本発明の分泌性細胞の１つであるハイブリド
ーマを培養する培養装置１の該略図を示す。[Execution 1! 1l11 FIG. 1 shows a schematic diagram of a culture apparatus 1 for culturing a hybridoma, which is one of the secretory cells of the present invention.
本発明は、基本的に中空糸膜７を内部に装填し側部にハ
イブリドーマ導入口８を形成した本体４と該本体４に培
地導入口９を有するキャップ５および培地導出口１０を
有するキャップ６を装填してなるハイブリドーマの培養
容器２と中空糸膜１４を内部に装填し側部に培地導出口
１５を形成した本体１１と該本体１１に培地導入口１６
を有するキャップ１２と端部を閉塞したキャップ１３を
装置してなる抗体の濃縮器３から構成される。The present invention basically consists of a main body 4 in which a hollow fiber membrane 7 is loaded and a hybridoma inlet 8 formed on the side, a cap 5 having a medium inlet 9 in the main body 4, and a cap 6 having a medium outlet 10. A hybridoma culture container 2 loaded with a main body 11 with a hollow fiber membrane 14 loaded inside and a medium outlet 15 formed on the side, and a medium inlet 16 in the main body 11.
The antibody concentrator 3 is comprised of a cap 12 having a cap 12 and a cap 13 having a closed end.
培養容器２と濃縮器３は、培地供給ライン１８、培地回
収ライン１９、培地排出ライン２０、培地循環ライン２
１により構成される培地循環回路内に配置される。The culture container 2 and the concentrator 3 are connected to a culture medium supply line 18, a culture medium recovery line 19, a culture medium discharge line 20, and a culture medium circulation line 2.
It is placed in a culture medium circulation circuit constituted by 1.
培養容器２と濃縮器３に使用する中空糸膜７．１４の材
質としては例えば、再生セルロース、セルロースアセテ
ート、ジエチルアミノエチルセルロース等のセルロース
系膜またはポリアクリロニトリル、ポリメチルメタクリ
レート、ポリスルホン、ポリカーボネート、ポリアミド
、ポリエチレン、ポリプロピレン等の合成膜からなり、
中空糸膜７は、ハイブリドーマを透過せず、抗体および
培地のみを透過するボアーサイズに設定され、他方、中
空糸膜１４は、モノクロナール抗体を透過せず培地のみ
を透過するボアーサイズに設定されている。Examples of the material for the hollow fiber membranes 7.14 used in the culture container 2 and the concentrator 3 include cellulose membranes such as regenerated cellulose, cellulose acetate, and diethylaminoethyl cellulose, or polyacrylonitrile, polymethyl methacrylate, polysulfone, polycarbonate, polyamide, and polyethylene. , made of synthetic membranes such as polypropylene,
The hollow fiber membrane 7 has a bore size that does not allow the hybridoma to pass through, but only the antibody and the medium, while the hollow fiber membrane 14 has a bore size that does not allow the monoclonal antibody to pass through, but only the medium. ing.
ハイブリドーマ、モノクロナール抗体のサイズによる異
なるが通常、中空糸膜７のボアーサイズは０．１〜０．
７ｕ、中空糸膜１４のボアーサイズは０．００３μ〜０
．０１μに設定される。Although it varies depending on the size of the hybridoma and monoclonal antibody, the bore size of the hollow fiber membrane 7 is usually 0.1 to 0.
7u, the bore size of the hollow fiber membrane 14 is 0.003μ to 0
．． It is set to 01μ.
図中、２２は培地の調整タンクで培地をハイブリドーマ
の培養条件に適した条件に調整して、培地供給ポンプＭ
、より、培地移送ライン２３を介して培地供給タンク１
７に供給する。In the figure, 22 is a medium adjustment tank that adjusts the medium to conditions suitable for hybridoma culture conditions, and a medium supply pump M.
, from the culture medium supply tank 1 via the culture medium transfer line 23.
Supply to 7.
培地回収ライン１９の途中には、培地管理センサー２４
が配置され、培地のＰＨ１乳酸、ＤＯを監視するもので
ある。A medium management sensor 24 is located in the middle of the medium recovery line 19.
is installed to monitor PH1 lactic acid and DO in the culture medium.
培地管理センサー２４と培地供給ポンプＭ。Medium management sensor 24 and medium supply pump M.
は、連動制御され、培地の交換時期が認められたら培地
供給ポンプＭ、が作動して培地調整タンク２２中の培地
を培地供給タンク２１中に送付するように制御すること
ができる。are controlled in conjunction with each other, and when it is determined that it is time to replace the medium, the medium supply pump M is activated to send the medium in the medium adjustment tank 22 into the medium supply tank 21.
更に培地供給ポンプＭ、は、培地排出ライン２０．２０
ａの途中に設けられた培地排出ポンプＭ２と連動制御さ
れ、培地調整タンク２２から培地循環回路内に供給され
る新鮮な培地と等量の使用済みの培地を排出するように
制御することができる。Furthermore, the medium supply pump M, is connected to the medium discharge line 20.20.
It is controlled in conjunction with the medium discharge pump M2 provided in the middle of a, and can be controlled to discharge the used medium in an amount equal to the fresh medium supplied into the medium circulation circuit from the medium adjustment tank 22. .
次に、本発明の使用方法について説明する。Next, a method of using the present invention will be explained.
ハイブリドーマの注入口８からハイブリドーマを培養容
器２の内部に導入する。Hybridomas are introduced into the culture container 2 through the hybridoma injection port 8.
培地供給タンク１７から培地を培地供給ライン１８、培
地導入口９を経て、培養容器２の本体４内に装填された
中空糸膜７の内部に導入する。The medium is introduced from the medium supply tank 17 into the hollow fiber membrane 7 loaded into the main body 4 of the culture container 2 via the medium supply line 18 and the medium inlet 9.
培地は、中空糸膜７の孔を通過して、中空糸膜７の表面
側のブリドーマに供給される。The medium passes through the holes in the hollow fiber membrane 7 and is supplied to the bridomomas on the surface side of the hollow fiber membrane 7.
ハイブリドーマが産生じたモノクロナール抗体と培地は
、再び中空糸膜７を通過し、キャップ６の培地導出口１
０、培地回収ライン１９゜モノクロナール抗体濃縮器３
の培地導入口１６を経て、中空糸膜１４内部に導入され
る。The monoclonal antibody produced by the hybridoma and the medium pass through the hollow fiber membrane 7 again and enter the medium outlet 1 of the cap 6.
0, Medium recovery line 19° Monoclonal antibody concentrator 3
The medium is introduced into the hollow fiber membrane 14 through the medium inlet 16 .
培地は中空糸膜１４を通過して、培地導出口１５を経て
、培地排出ライン２０、培地循環ライン２１を経て、培
地供給タンク１７へ戻り。The medium passes through the hollow fiber membrane 14, passes through the medium outlet 15, passes through the medium discharge line 20, and the medium circulation line 21, and returns to the medium supply tank 17.
培地供給ライン１８を経て、再び培養容器２に導入され
る。The medium is introduced into the culture container 2 again through the medium supply line 18.
または、ハイブリドーマが産生したモノクロナール抗体
と培地は、第２図に示すモノクロナール抗体濃縮器３ａ
を用いて処理することができる。Alternatively, the monoclonal antibody produced by the hybridoma and the culture medium can be stored in the monoclonal antibody concentrator 3a shown in FIG.
It can be processed using
すなわち前述と同様に、再び中空糸膜７を通過し、キャ
ップ６の培地導出口１０、培地回収ライン１９、モノク
ロナール抗体濃縮器３ａの培地導入口２５を経て、中空
糸膜１４ａの外部に導入される。That is, in the same way as described above, it passes through the hollow fiber membrane 7 again, passes through the medium outlet 10 of the cap 6, the medium recovery line 19, and the medium inlet 25 of the monoclonal antibody concentrator 3a, and is introduced to the outside of the hollow fiber membrane 14a. be done.
培地は中空糸膜１４ａを通過して、培地導出０２６を経
て、培地排出ライン２０、培地循環ライン２１を経て、
培地供給タンク１７に戻り、培地供給ライン１８を経て
、再び培養容器２に導入される。The medium passes through the hollow fiber membrane 14a, passes through the medium outlet 026, passes through the medium discharge line 20, and the medium circulation line 21.
It returns to the culture medium supply tank 17, passes through the culture medium supply line 18, and is introduced into the culture container 2 again.
以上のようにして、培地を循環させて培養容器２内の中
空糸膜７の表面側のハイブリドーマに供給し、バイブリ
ドーマの増殖およびモノクロナール抗体の産生を誘起さ
せる。As described above, the medium is circulated and supplied to the hybridomas on the surface side of the hollow fiber membrane 7 in the culture container 2, thereby inducing proliferation of the hybridomas and production of monoclonal antibodies.
ハイブリドーマより産生されたモノクロナル抗体は、培
地と共に培地回収ライン１９を経て、抗体濃縮容器３の
中空糸膜１４内または中空糸膜１４ａ外へ導入される。The monoclonal antibody produced by the hybridoma is introduced into the hollow fiber membrane 14 or outside the hollow fiber membrane 14a of the antibody concentration container 3 through the medium recovery line 19 together with the medium.
中空糸膜１４．１４ａは、モノクロナール抗体の透過を
阻止できる程度のボアーサイズに設定されているので、
モノクロナール抗体は中空糸膜１４内に滞留して濃縮ま
たは、中空糸膜１４ａ外に滞留して濃縮されることにな
る。Since the hollow fiber membrane 14.14a is set to have a bore size that can block the permeation of monoclonal antibodies,
The monoclonal antibody will stay within the hollow fiber membrane 14 and be concentrated, or stay outside the hollow fiber membrane 14a and be concentrated.
培地回収ライン１９の途中に配置した培地管理センサー
２４により、培地の交換時期が認められたならば、培地
排出ライン２０ａを経て、使用済みの培地を抜き出しな
がら培地調整タンク２２から、新鮮な培地を培地供給タ
ンク１７に導入する。When the culture medium management sensor 24 placed in the middle of the culture medium collection line 19 recognizes that it is time to replace the culture medium, fresh culture medium is extracted from the culture medium adjustment tank 22 through the culture medium discharge line 20a while removing the used culture medium. The medium is introduced into the medium supply tank 17.
すなわち、培地管理センサー２４と培地供給ポンプＭ、
を連動制御し、あらかじめセットした培地管理設定値以
下または以上になったら、培地供給ポンプＭ３が駆動し
、所定の条件に調整した培地を培地供給タンク１７に供
給して培地循環回路内を循環させ、ハイブリドーマの増
殖およびモノクロナール抗体の産生を誘起させるもので
ある。That is, the culture medium management sensor 24 and the culture medium supply pump M,
When the medium becomes below or above a preset medium management set value, the medium supply pump M3 is activated to supply the medium adjusted to predetermined conditions to the medium supply tank 17 and circulate it in the medium circulation circuit. , which induces hybridoma proliferation and monoclonal antibody production.
この場合、培地排出ポンプＭ２と培地供給ポンプＭ、を
連動制御して、前記の所定の条件に調整した培地の供給
量と等量の使用済みの培地を排出するようにする。培養
装置１内を循環している培地の質および量を一定維持し
て、常に所定の条件に調整された培地をハイブリドーマ
に供給、ハイブリドーマの増殖、モノクロナール抗体の
産生効率を高めることができる。In this case, the medium discharge pump M2 and the medium supply pump M are controlled in conjunction with each other to discharge the used medium in an amount equal to the supplied amount of the medium adjusted to the predetermined conditions. By maintaining the quality and quantity of the medium circulating in the culture device 1 constant, the medium adjusted to predetermined conditions can be constantly supplied to the hybridomas, thereby increasing hybridoma growth and monoclonal antibody production efficiency.
所定量のモノクロナール抗体が中空糸膜１４内に蓄積し
たと認められたならば、濃縮液導出口１３のキャップを
開け、モノクローナル抗体を回収、または濃縮器３を新
たな濃縮器と交換して、培養を継続することができる。When it is recognized that a predetermined amount of monoclonal antibody has accumulated in the hollow fiber membrane 14, the cap of the concentrate outlet 13 is opened and the monoclonal antibody is collected, or the concentrator 3 is replaced with a new one. , culture can be continued.
［発明の効果］
以上説明したように、培養容器２中に、分泌性細胞の増
殖細胞のみ、濃縮器３に分泌性細胞により産生じた分泌
物のみを収集することによって、分泌性細胞と分泌物の
分離作業を省略することができ、分泌性細胞を高密度の
状態で、分離収集することができる等の効果を有する優
れた発明である。[Effects of the Invention] As explained above, by collecting only the proliferating cells of secretory cells in the culture vessel 2 and only the secretions produced by the secretory cells in the concentrator 3, secretory cells and secretory substances can be collected. This is an excellent invention that has the following effects: it is possible to omit the work of separating substances, and it is possible to separate and collect secretory cells in a high density state.
第１図は、本発明の培養装置を示す該略図、第２図は、
分泌物の濃縮器の他の実施例を示す該略図である。
図中、１は培養装置、２は培養容器、３．３ａは分泌物
の濃縮器、１７は培地供給タンク、２２は培地調整タン
ク、２４は培地管理センサーを示す。
特許出願人 川澄化学工業株式会社
手続補正書
（自発）
補正の内容
発明の名称
培 養 装 置
補正をする者
事件との関係
住所（居所）
〒１４０FIG. 1 is a schematic diagram showing the culture apparatus of the present invention, and FIG.
Figure 3 is a diagram showing another embodiment of a secretion concentrator; In the figure, 1 is a culture device, 2 is a culture container, 3.3a is a secretion concentrator, 17 is a medium supply tank, 22 is a medium adjustment tank, and 24 is a medium management sensor. Patent applicant Kawasumi Chemical Industry Co., Ltd. Procedural amendment (voluntary) Contents of amendment Name of invention Cultivation Device Person making the amendment Address related to the case (residence) 140
Claims (2)
た分泌物を濃縮する培養装置であって、分泌物を透過す
る中空糸膜を本体内部に装填した分泌性細胞の培養容器
と分泌物の透過を阻止する中空糸膜を本体内部に装填し
た分泌物の濃縮容器を、培地供給ライン、培地回収ライ
ン、培地排出ライン、培地循環ラインからなる培地循環
回路の途中に配置したことを特徴とする培養装置。(1) A culture device for culturing secretory cells and concentrating secretions produced from the secretory cells, comprising a secretory cell culture vessel equipped with a hollow fiber membrane that permeates the secretions inside the main body. The container for concentrating secretions, which is equipped with a hollow fiber membrane inside the main body to prevent the permeation of secretions, is placed in the middle of the medium circulation circuit consisting of the medium supply line, medium recovery line, medium discharge line, and medium circulation line. Characteristic culture equipment.
供給ポンプＭ＿１を設けた培地供給ラインの上流側に、
途中に培地移送ポンプＭ＿２を設けた培地移送ラインを
介して培地調整タンクを連結した培地供給タンクを設け
、培地回収ラインの途中に設けた培地管理センサーと前
記培地移送ポンプＭ＿３を連動制御するとともに培地排
出ラインの途中に設けた培地排出ポンプＭ＿２と前記培
地移送ポンプＭ＿３を連動制御することを特徴とする培
養装置。(2) In the culture apparatus according to item 1 above, on the upstream side of the culture medium supply line in which the culture medium supply pump M_1 is provided midway,
A culture medium supply tank is provided which is connected to a culture medium adjustment tank via a culture medium transfer line with a culture medium transfer pump M_2 in the middle, and the culture medium management sensor provided in the middle of the culture medium recovery line and the medium transfer pump M_3 are controlled in conjunction with each other. A culture apparatus characterized in that a culture medium discharge pump M_2 provided in the middle of a discharge line and the culture medium transfer pump M_3 are controlled in conjunction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63201449A JP2750581B2 (en) | 1988-08-12 | 1988-08-12 | Culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63201449A JP2750581B2 (en) | 1988-08-12 | 1988-08-12 | Culture device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0253479A true JPH0253479A (en) | 1990-02-22 |
| JP2750581B2 JP2750581B2 (en) | 1998-05-13 |
Family
ID=16441273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63201449A Expired - Fee Related JP2750581B2 (en) | 1988-08-12 | 1988-08-12 | Culture device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2750581B2 (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003510068A (en) * | 1999-09-30 | 2003-03-18 | ユニサーチ リミテツド | Method and apparatus for culturing cells |
| JP2011103882A (en) * | 2009-11-16 | 2011-06-02 | Shuhei Nakaji | Cell culture apparatus, and method and program for culturing cell |
| CN112251333A (en) * | 2020-10-12 | 2021-01-22 | 北京诺德观呈医疗科技有限公司 | Device and method for purifying cell exosomes |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60137280A (en) * | 1983-12-26 | 1985-07-20 | Nippon Zenyaku Kogyo Kk | Apparatus for concentrating product produced by cell |
| JPS6249904A (en) * | 1985-08-30 | 1987-03-04 | Sumitomo Bakelite Co Ltd | Filtration method |
-
1988
- 1988-08-12 JP JP63201449A patent/JP2750581B2/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60137280A (en) * | 1983-12-26 | 1985-07-20 | Nippon Zenyaku Kogyo Kk | Apparatus for concentrating product produced by cell |
| JPS6249904A (en) * | 1985-08-30 | 1987-03-04 | Sumitomo Bakelite Co Ltd | Filtration method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003510068A (en) * | 1999-09-30 | 2003-03-18 | ユニサーチ リミテツド | Method and apparatus for culturing cells |
| JP2011103882A (en) * | 2009-11-16 | 2011-06-02 | Shuhei Nakaji | Cell culture apparatus, and method and program for culturing cell |
| CN112251333A (en) * | 2020-10-12 | 2021-01-22 | 北京诺德观呈医疗科技有限公司 | Device and method for purifying cell exosomes |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2750581B2 (en) | 1998-05-13 |
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