JPH0240041B2 - - Google Patents
Info
- Publication number
- JPH0240041B2 JPH0240041B2 JP62061642A JP6164287A JPH0240041B2 JP H0240041 B2 JPH0240041 B2 JP H0240041B2 JP 62061642 A JP62061642 A JP 62061642A JP 6164287 A JP6164287 A JP 6164287A JP H0240041 B2 JPH0240041 B2 JP H0240041B2
- Authority
- JP
- Japan
- Prior art keywords
- fusarium
- live
- bacteria
- viable
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000223218 Fusarium Species 0.000 claims description 33
- 238000002360 preparation method Methods 0.000 claims description 21
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 20
- 239000002612 dispersion medium Substances 0.000 claims description 8
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims description 6
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 6
- 229910021536 Zeolite Inorganic materials 0.000 claims description 6
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 229960002920 sorbitol Drugs 0.000 claims description 6
- 239000010457 zeolite Substances 0.000 claims description 6
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 3
- 229930195712 glutamate Natural products 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 description 19
- 230000001580 bacterial effect Effects 0.000 description 18
- 244000017020 Ipomoea batatas Species 0.000 description 11
- 235000002678 Ipomoea batatas Nutrition 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 201000010099 disease Diseases 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 240000008067 Cucumis sativus Species 0.000 description 4
- 235000009849 Cucumis sativus Nutrition 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000009777 vacuum freeze-drying Methods 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 3
- 235000013923 monosodium glutamate Nutrition 0.000 description 3
- 239000004223 monosodium glutamate Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000005909 Kieselgur Substances 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 235000020183 skimmed milk Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 240000005528 Arctium lappa Species 0.000 description 1
- 235000003130 Arctium lappa Nutrition 0.000 description 1
- 235000008078 Arctium minus Nutrition 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000879839 Fusarium oxysporum f. sp. batatas Species 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000155437 Raphanus sativus var. niger Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 208000000260 Warts Diseases 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 235000012907 honey Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940066779 peptones Drugs 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 201000010153 skin papilloma Diseases 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Description
［産業上の利用分野］
この発明は、フザリウムの生菌を、生存状態の
まま基剤に保持して乾燥した生菌製剤に関する。
［発明の背景］
サツマイモのつる割れ病は、毒性のフザリウム
菌（Fusarium oxysporum f.sp.batatas）によつ
て引き起こされることが既に知られている。
本件発明者は、非病原性のフザリウム菌
（Fusarium oxysporum）をサツマイモの苗の基
部の切口に接種することによつて、上記サツマイ
モのつる割病を顕著に抑える効果があることを発
見した。同時に、この効果は実際のサツマイモ栽
培に於ても高い実用性を備えていることが確認で
きた。
さらに、この非病原性のフザリウム菌は、他の
作物のフザリウム病の抑制にも明確な有効性が認
められ、広くフザリウム病に対する微生物防除手
段として有効である。
上記フザリウム菌を用いたサツマイモ及びその
他の作物の試験は、液体培養した生菌をそのまま
用いて実施した。
しかし、液体培養されたままのフザリウム生菌
は、次のような理由から実用上の取扱や安定性に
問題がある。即ち、(1)液状であるため、搬送や現
場での接種作業に不便である、(2)生菌が増殖培地
の中に存在しているため、雑菌が混入して繁殖し
やすく、不安定である。このため、管理に細心の
注意が必要となる、(3)フザリウム菌は振とう培養
によつて多くの芽胞が形成されるが、静置保管す
ると菌体は菌糸体となるため、接種に適さなくな
る、等の理由である。この問題は、単に使用し難
いという問題にとどまらず、本来のつる割病の抑
制効果そのものを減殺させる可能性を含んでい
る。
フザリウム菌体の長期保存法としては、これま
で凍結乾燥法、流動パラフイン重層法、土壌保存
法、風乾法等が実施されてきたが、これらは何れ
も標本としての保存法の域を出ず、生菌製剤の製
造法としては極めて生産性が低く、得られる製剤
の品質も不安定である。
この発明の目的は、上記フザリウム菌のつる割
病に対する効果に着目すると共に、一般農家が手
軽に扱え、なおかつ生菌としての活性と安定性と
を同時に備えたフザリウム生菌製剤を提供するこ
とにある。
［問題を解決するための手段］
即ち、本件第一及び第二の発明の共通する基本
的な特徴は、原料となる生菌にフザリウム生菌を
用いることである。そして第一の発明によるフザ
リウム菌の生菌製剤は、上記フザリウムの生菌体
をゼオライト系の基材に吸着させ、自然乾燥させ
たものである。また、第二の発明によるフザリウ
ム生菌製剤は、Ｄ―ソルビトールを主体とし、こ
れにグルタミン酸塩を少量添加した分散媒にフザ
リウムの生菌体を分散し、真空凍結乾燥させたも
のである。
液体培養により得られたフザリウム菌体を濃縮
し、自然乾燥するに当り、基材としてゼオライト
系資材を用いることにより、また、真空凍結乾燥
に際し、分散媒として、Ｄ―ソルビトールに少量
のグルタミン酸塩を添加した分散媒を用いること
により、本菌の生存率が飛躍的に向上する。こう
して得られた生菌製剤は常温以下、望ましくは５
℃前後の温度下において長期間の保存が可能であ
る。
さらに、使用時に水に溶かす場合は懸濁状に分
散できるため、非常に取扱いが容易である。
この発明において使用するフザリウム菌は健全
なサツマイモの導管から分離したFusarium
oxysporumである。この菌体はキユウリ、ユウ
ガオ、マクワウリ、ダイコン、キヤベツ、トマ
ト、ゴボウ、ネギ、レタス、ホウレンソウおよび
カーネーシヨンなどに対して病原性のない非病原
性菌で、自然界に広く分布する。また、本菌はサ
ツマイモ、キユウリ、ユウガオ、マクワウリ、ト
マトなどのフザリウム病を抑制する。
なお、第二の発明において、分散媒の中に、ス
キムミルクを添加するとより効果的である。
［実施例］
次に、この発明の実施例について具体的に説明
する。
実施例 １
まず、フザリウム菌体をシヨ糖、あるいはブド
ウ糖加用ジヤガイモ煎汁液体培地で振とう培養し
（ジヤーフアーメンター培養法を用いてもよい）、
上記菌体（芽胞―budcell）を含む懸濁液を得た。
これにゼオライト系基材としてスーパーＺ、朝日
ゼオライト、グリーンキヤツチヤー、クレー、タ
ルクCTA（以上何れも商品名、以下に同じ）及び
珪藻土をそれぞれ17.5ｇ／100mlの割合で添加し、
3000rpmの回転数で５分間遠心分離を行つた。
上澄液を除去し、沈澱した菌体および吸着剤を
よく混合し、バツトの上に拡げ、27.5、15.0、5.0
℃の通風定温器の中で含水量10％以下になるまで
自然乾燥し、第一の発明による生菌製剤を製造し
た。
[Industrial Application Field] The present invention relates to a viable bacterial preparation in which Fusarium viable bacteria are held in a base in a viable state and dried. [Background of the Invention] It is already known that vine crack disease of sweet potatoes is caused by the toxic Fusarium fungus (Fusarium oxysporum f.sp.batatas). The inventors of the present invention have discovered that inoculating non-pathogenic Fusarium oxysporum into the cut end of the base of sweet potato seedlings has the effect of significantly suppressing the above-mentioned vine splitting disease of sweet potatoes. At the same time, it was confirmed that this effect is highly practical in actual sweet potato cultivation. Furthermore, this non-pathogenic Fusarium fungus is clearly effective in suppressing Fusarium disease in other crops, and is widely effective as a microbial control measure against Fusarium disease. The above-mentioned tests on sweet potatoes and other crops using Fusarium fungi were carried out using liquid-cultured live bacteria as they were. However, living Fusarium bacteria that have been cultured in liquid have problems in practical handling and stability for the following reasons. In other words, (1) it is in a liquid state, making it inconvenient for transportation and on-site inoculation work, and (2) since live bacteria are present in the growth medium, it is easy for bacteria to get mixed in and multiply, making it unstable. It is. For this reason, careful management is required. (3) Fusarium bacteria forms many spores when cultured with shaking, but when stored statically, the bacterial bodies turn into mycelium, making them unsuitable for inoculation. The reason is that it disappears. This problem is not just that it is difficult to use, but also includes the possibility that the original effect of suppressing vine wart disease itself may be diminished. Up to now, methods for long-term preservation of Fusarium cells have been used, such as freeze-drying, liquid paraffin layering, soil preservation, and air-drying, but all of these methods go beyond preserving specimens. As a method for producing viable bacterial preparations, productivity is extremely low, and the quality of the resulting preparations is unstable. The purpose of the present invention is to focus on the effect of Fusarium fungi on vine splitting disease, and to provide a live Fusarium preparation that can be easily handled by general farmers and has both the activity and stability of living fungi. be. [Means for solving the problem] That is, the basic feature that the first and second inventions have in common is that Fusarium live bacteria are used as the raw material. The viable Fusarium bacterial preparation according to the first invention is obtained by adsorbing the above-mentioned viable Fusarium cells onto a zeolite base material and naturally drying the same. Further, the live Fusarium preparation according to the second invention is obtained by dispersing live Fusarium cells in a dispersion medium containing D-sorbitol as a main ingredient and adding a small amount of glutamate, and vacuum freeze-drying. When concentrating Fusarium cells obtained by liquid culture and drying naturally, a zeolite-based material is used as a base material. Also, during vacuum freeze-drying, a small amount of glutamate is added to D-sorbitol as a dispersion medium. By using the added dispersion medium, the survival rate of this bacterium is dramatically improved. The viable bacterial preparation thus obtained is kept at room temperature or below, preferably at
It can be stored for long periods at temperatures around ℃. Furthermore, when it is dissolved in water at the time of use, it can be dispersed into a suspension, making it extremely easy to handle. The Fusarium fungus used in this invention is Fusarium isolated from the tubes of healthy sweet potatoes.
It is oxysporum. This fungus is a non-pathogenic bacterium that is not pathogenic to cucurbits, cucumbers, daikon radish, cabbage, tomatoes, burdock, green onions, lettuce, spinach, carnations, etc., and is widely distributed in nature. In addition, this fungus suppresses Fusarium disease on sweet potatoes, cucumbers, cucumbers, cucumbers, tomatoes, etc. In addition, in the second invention, it is more effective to add skim milk to the dispersion medium. [Example] Next, an example of the present invention will be specifically described. Example 1 First, Fusarium cells were cultured with shaking in a liquid culture medium containing sucrose or glucose-added potato decoction (the fermenter culture method may also be used),
A suspension containing the above-mentioned bacterial cells (spores) was obtained.
To this, Super Z, Asahi Zeolite, Green Catcher, Clay, Talc CTA (all of the above are trade names, the same applies below) and diatomaceous earth were added as zeolite base materials at a rate of 17.5 g/100 ml, respectively.
Centrifugation was performed for 5 minutes at a rotation speed of 3000 rpm. Remove the supernatant, mix the precipitated bacterial cells and adsorbent well, spread it on the vat, and add 27.5, 15.0, 5.0
The viable bacterial preparation according to the first invention was produced by air drying in a ventilation incubator at ℃ until the water content was 10% or less.
【表】
その12日後に、これら生菌製剤についてフザリ
ウム菌分離用駒田培地を用いて、希釈平板法によ
り生存菌数を調べた。この結果を表１に示す。
実施例 ２
上記と同様の方法で得られた培養液にゼオライ
ト系基材としてスーパーＺを17.5ｇ／100ml加え、
以下上記実施例と同様にして第一の発明によるフ
ザリウム生菌製剤を製造した。
その後、５℃、25℃及び37℃の温度下で200日
間保存し、その間駒田培地を用いて希釈平板法に
より単位容積（１ml）当りの経時的な菌数を調べ
た。この結果を第１図のグラフに点線で示した。
実施例 ３
上記実施例１と同様の方法で、フザリウム菌数
107個／mlの培養懸濁液、及び基材として珪藻土、
朝日ゼオライト、スーパーＺをそれぞれ用いたフ
ザリウム生菌製剤を製造した。
これを10倍と102倍の水に分散して、懸濁状と
したものにそれぞれ50株ずつのサツマイモの品種
「ベニコマチ」種の苗を17時間浸漬した。その直
後の昭和61年−５月20日に、これらの苗を茨城県
水戸市上国井町の茨城県農業試験場内の人工汚染
圃場に植え付け、同年10月15日に掘り取つた。こ
の間、同年６月26日と収穫時にサツマイモのつる
割病の発病株率（枯死株率）を調べた。また、苗
50株当りの単位収量を調べ、これらの結果を表２
に示した。[Table] After 12 days, the number of viable bacteria was examined using Komada medium for Fusarium isolation using the dilution plate method for these viable bacterial preparations. The results are shown in Table 1. Example 2 17.5g/100ml of Super Z was added as a zeolite base material to the culture solution obtained in the same manner as above,
Thereafter, a live Fusarium preparation according to the first invention was produced in the same manner as in the above example. Thereafter, it was stored at temperatures of 5°C, 25°C, and 37°C for 200 days, during which time the number of bacteria per unit volume (1 ml) was determined by the dilution plate method using Komada medium. This result is shown in the graph of FIG. 1 by a dotted line. Example 3 The number of Fusarium bacteria was determined in the same manner as in Example 1 above.
10 7 cells/ml culture suspension, and diatomaceous earth as a substrate,
Fusarium live bacterial preparations using Asahi zeolite and Super Z were manufactured. This was dispersed in 10 times and 10 2 times the amount of water, and 50 seedlings of the sweet potato variety ``Benikomachi'' were immersed in each suspension for 17 hours. Immediately thereafter, on May 20, 1986, these seedlings were planted in an artificially contaminated field within the Ibaraki Prefectural Agricultural Experiment Station in Kamikunii-cho, Mito City, Ibaraki Prefecture, and dug up on October 15 of the same year. During this period, the incidence of disease (withering rate) of sweet potato vine split disease was investigated on June 26 of the same year and at the time of harvest. Also, seedlings
The unit yield per 50 plants was investigated and the results are shown in Table 2.
It was shown to.
【表】
なお、比較のため、フザリウム生菌製剤による
処理を行わなかつた苗を植え付けて同じ圃場で同
時にサツマイモを栽培し、これについても上記の
事項を調べ、この結果を表２に併記した。
上記の結果から明らかな通り、第一の発明によ
るフザリウム生菌製剤は、サツマイモのつる割病
に顕著な効果が認められる。
実施例 ４
上記実施例１と同様にして得られた菌体培養液
を遠心分離し、濃縮した菌体を得た。これにＤ―
ソルビトール10％、グルタミン酸ナトリウム１％
及び残部が水からなる分散媒、並びにＤ―ソルビ
トール10％、スキムミルク10％、グルタミン酸ナ
トリウム１％及び残部が水からなる分散媒を上記
培養液に対して20％容積添加し、これをバイアル
瓶に詰め、−20℃で予備凍結後、−５℃で真空凍結
乾燥し、第二の発明によるフザリウム菌の生菌製
剤を製造した。
その直後の菌体の生存率を希釈平板法により調
べ、その結果を表３の最上段と２段目に示した。
さらに、分散媒にグルタミン酸ナトリウムを添
加しない場合、分散媒中のＤ―ソルビトールに代
えて、糖、ペプトン類としてハチミツ、マルトー
ス、スクロース等を用いて同様に真空凍結乾燥し
た生菌製剤を作り、凍結乾燥直後の菌体の生存率
を調べた。これらの結果を表３の３段目以降に示
した。[Table] For comparison, we planted seedlings that were not treated with the Fusarium live bacteria preparation and cultivated sweet potatoes at the same time in the same field, and the above matters were also investigated, and the results are also listed in Table 2. As is clear from the above results, the Fusarium viable bacterial preparation according to the first invention has a remarkable effect on sweet potato vine splitting disease. Example 4 A bacterial cell culture solution obtained in the same manner as in Example 1 above was centrifuged to obtain concentrated bacterial cells. D- for this
Sorbitol 10%, monosodium glutamate 1%
Add a dispersion medium consisting of 10% D-sorbitol, 10% skim milk, 1% monosodium glutamate, and the balance water to the above culture solution in a volume of 20%, and add this to the vial. The mixture was packed, pre-frozen at -20°C, and then vacuum freeze-dried at -5°C to produce a live Fusarium preparation according to the second invention. Immediately thereafter, the survival rate of the bacterial cells was examined by the dilution plate method, and the results are shown in the top and second rows of Table 3. Furthermore, when monosodium glutamate is not added to the dispersion medium, a live bacterial preparation is prepared by vacuum freeze-drying in the same manner using honey, maltose, sucrose, etc. as sugars and peptones in place of D-sorbitol in the dispersion medium, and frozen. The survival rate of bacterial cells was examined immediately after drying. These results are shown in the third and subsequent rows of Table 3.
【表】
以上の結果から明らかな通り、表３の上２段に
示す第二の発明による実施例では、他のものにく
らべて、菌の生存率が極めて高いことが確認出来
る。
実施例 ５
上記実施例４の表３に於て２段目に示した生菌
製剤を容積10mlのバイアル瓶に９mlずつ分注し、
上記実施例４と同じ条件で真空凍結乾燥した。そ
の後、一部の試料についてはシリカゲル、濾紙、
綿等の吸湿剤と共に真空封着したものと、真空封
着しなかつたものとを、それぞれ５℃、25℃、37
℃の温度下で136日間保存し、そのの間の各試料
の単位容積当りの生菌数を計数した。この結果を
第２図に示す。なお、実線は真空封着したものの
結果を、点線は真空封着しないものの結果を示
す。
以上の結果から明らかな通り、温度５℃のもと
では、何れの場合も136日後に高い生存菌数が確
認された。
［発明の効果］
以上説明した通り、この発明の生菌製剤によれ
ば、病原性のフザリウム菌による作物のつる割病
の発病防止に効果があり、これによつて作物の収
量の増大を図ることが出来る。
さらに、第一の発明、第二の発明何れによるも
のでも、常温以下の温度で長期の保存に耐えるこ
とが出来ると共に、使用時には、水中に容易に分
散するため、手軽に使用することができる。[Table] As is clear from the above results, it can be confirmed that in the example according to the second invention shown in the upper two rows of Table 3, the survival rate of bacteria is extremely high compared to the other examples. Example 5 The viable bacterial preparation shown in the second row of Table 3 of Example 4 was dispensed into 10 ml vials in 9 ml portions,
Vacuum freeze-drying was carried out under the same conditions as in Example 4 above. After that, for some samples, silica gel, filter paper,
The one that was vacuum sealed with a moisture absorbent such as cotton and the one that was not vacuum sealed were heated at 5°C, 25°C, and 37°C, respectively.
The samples were stored at ℃ for 136 days, during which time the number of viable bacteria per unit volume of each sample was counted. The results are shown in FIG. Note that the solid line shows the results with vacuum sealing, and the dotted line shows the results without vacuum sealing. As is clear from the above results, a high number of viable bacteria was confirmed after 136 days in all cases at a temperature of 5°C. [Effects of the Invention] As explained above, the live bacterial preparation of the present invention is effective in preventing the onset of vine splitting disease in crops caused by pathogenic Fusarium fungi, thereby increasing crop yields. I can do it. Furthermore, both the first invention and the second invention can withstand long-term storage at temperatures below room temperature, and can be easily used because they are easily dispersed in water when used.
第１図は実施例２に於ける生菌製剤の保存日数
と生存菌数の関係を示すグラフ、第２図は実施例
５に於ける生菌製剤の保存日数と生存菌数の関係
を示すグラフである。
Figure 1 is a graph showing the relationship between the number of days the live bacteria preparation was stored and the number of viable bacteria in Example 2, and Figure 2 is a graph showing the relationship between the number of days the live bacteria preparation was stored and the number of viable bacteria in Example 5. It is a graph.
Claims (1)
吸着させ、自然乾燥させたことを特徴とするフザ
リウム生菌製剤。 ２ Ｄ―ソルビトールを主体とし、これに少量の
グルタミン酸塩を添加した分散媒にフザリウムの
生菌体を分散し、真空凍結乾燥させたことを特徴
とするフザリウム生菌製剤。[Scope of Claims] 1. A live Fusarium preparation characterized by adsorbing living Fusarium cells onto a zeolite base material and allowing it to air dry. 2. A live Fusarium preparation, characterized in that live Fusarium cells are dispersed in a dispersion medium containing D-sorbitol and a small amount of glutamate added thereto, and then vacuum freeze-dried.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62061642A JPS63227507A (en) | 1987-03-17 | 1987-03-17 | Agent containing living fusarium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62061642A JPS63227507A (en) | 1987-03-17 | 1987-03-17 | Agent containing living fusarium |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63227507A JPS63227507A (en) | 1988-09-21 |
| JPH0240041B2 true JPH0240041B2 (en) | 1990-09-10 |
Family
ID=13177070
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62061642A Granted JPS63227507A (en) | 1987-03-17 | 1987-03-17 | Agent containing living fusarium |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS63227507A (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02240008A (en) * | 1989-03-14 | 1990-09-25 | Nok Corp | Plant culturing promoter and promoting method of plant culturing |
| JP2578302B2 (en) * | 1992-12-25 | 1997-02-05 | 日本たばこ産業株式会社 | Novel microorganism, soil disease controlling agent containing the microorganism, and soil disease controlling method using the same |
| JPH06247822A (en) * | 1992-12-28 | 1994-09-06 | Japan Tobacco Inc | Viable cell-containing formulation and its production |
| JP3697175B2 (en) | 2001-04-26 | 2005-09-21 | クミアイ化学工業株式会社 | Agricultural hydrating composition, method for producing the same, and method for storing the same |
| JP5000133B2 (en) * | 2005-12-28 | 2012-08-15 | 北興化学工業株式会社 | Microbial pesticide formulation |
| US9084434B2 (en) * | 2006-09-27 | 2015-07-21 | Little Calumet Holdings Llc | Probiotic oral dosage forms |
-
1987
- 1987-03-17 JP JP62061642A patent/JPS63227507A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63227507A (en) | 1988-09-21 |
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