JPH0227975A - Tool for fungus inoculation and fungus inoculation using same - Google Patents
Tool for fungus inoculation and fungus inoculation using sameInfo
- Publication number
- JPH0227975A JPH0227975A JP17589288A JP17589288A JPH0227975A JP H0227975 A JPH0227975 A JP H0227975A JP 17589288 A JP17589288 A JP 17589288A JP 17589288 A JP17589288 A JP 17589288A JP H0227975 A JPH0227975 A JP H0227975A
- Authority
- JP
- Japan
- Prior art keywords
- inoculation
- tool
- culture medium
- tip
- fungus inoculation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000011081 inoculation Methods 0.000 title claims abstract description 38
- 241000233866 Fungi Species 0.000 title abstract 6
- 239000001963 growth medium Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 8
- 239000000835 fiber Substances 0.000 claims abstract description 7
- 229920000742 Cotton Polymers 0.000 abstract description 7
- 235000017166 Bambusa arundinacea Nutrition 0.000 abstract description 3
- 235000017491 Bambusa tulda Nutrition 0.000 abstract description 3
- 235000015334 Phyllostachys viridis Nutrition 0.000 abstract description 3
- 239000011425 bamboo Substances 0.000 abstract description 3
- 229910052751 metal Inorganic materials 0.000 abstract description 3
- 239000002184 metal Substances 0.000 abstract description 3
- 239000000853 adhesive Substances 0.000 abstract description 2
- 230000001070 adhesive effect Effects 0.000 abstract description 2
- 238000007796 conventional method Methods 0.000 abstract description 2
- 229920002994 synthetic fiber Polymers 0.000 abstract description 2
- 239000012209 synthetic fiber Substances 0.000 abstract description 2
- 239000000057 synthetic resin Substances 0.000 abstract description 2
- 229920003002 synthetic resin Polymers 0.000 abstract description 2
- 239000002023 wood Substances 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract 2
- 241001330002 Bambuseae Species 0.000 abstract 1
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 21
- 229910052697 platinum Inorganic materials 0.000 description 10
- 229920001817 Agar Polymers 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 7
- 239000008272 agar Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 244000153158 Ammi visnaga Species 0.000 description 5
- 235000010585 Ammi visnaga Nutrition 0.000 description 5
- 244000082204 Phyllostachys viridis Species 0.000 description 2
- 229920001875 Ebonite Polymers 0.000 description 1
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910001120 nichrome Inorganic materials 0.000 description 1
- 150000003057 platinum Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
Abstract
Description
【発明の詳細な説明】
（技術分野）
この発明は、植菌用具とこれを用いた培地への植苗方法
に関するものである。さらに詳しくは、培地表面を損出
することがなく、コロニー数の多い植菌を可能とする新
しい植菌用具と植菌方法に関するものである。DETAILED DESCRIPTION OF THE INVENTION (Technical Field) The present invention relates to a seedling inoculation tool and a method for planting seedlings in a medium using the same. More specifically, the present invention relates to a new inoculation tool and inoculation method that enables inoculation with a large number of colonies without damaging the surface of the medium.
（従来の技術とその課題）
従来より、医学、薬学の研究や発酵工業等の開発研究に
おいて、培地に菌を植付けて培養することが行われてき
ており、この菌の植付けには、通常、寒天培地の表面に
白金耳、木製つまようじによって菌を塗布することが行
われてきている。(Conventional technology and its issues) Traditionally, in medical and pharmaceutical research and development research in the fermentation industry, bacteria have been planted and cultured in culture media. Bacteria have been applied to the surface of an agar medium using a platinum loop or a wooden toothpick.
しかしながら、この従来の白金耳や木製つまようじを用
いる植菌方法においては、これら白金耳や木製つまよう
じからなる植菌用具によって寒天培地の表面を傷つけや
すく、コロニーの分離が不可能になって生成するコロニ
ーの個数が少ないという欠点があった。However, in this conventional inoculation method using platinum loops and wooden toothpicks, these inoculation tools made of platinum loops and wooden toothpicks tend to damage the surface of the agar medium, making it impossible to separate colonies and forming colonies. The disadvantage was that there were only a few.
たとえば第２図に示したように、植菌用の白金耳として
は、エボナイト柄からなる握り部（ア）と、その先の白
金線（現在は電熱用のニクロム線が用いられる） （イ
）とからなり、この白金線（イ）の先端はループ状部（
つ）を形成しているものが知られている。この白金耳は
、寒天培地表面に菌を塗布植菌する場合に不可欠なもの
となってもいる。しかしながら、このループ状部（つ）
を有する白金耳を用いる場合にも、培地表面への塗布時
にどうしてもこの表面を傷つけてしまうことを避けるこ
とができなかっな。For example, as shown in Figure 2, the platinum loop for inoculation consists of a grip made of ebonite handle (A) and a platinum wire at the end (Currently, nichrome wire for electric heating is used) (B) The tip of this platinum wire (A) is a loop-shaped part (
It is known what forms the This platinum loop is essential when applying and inoculating bacteria onto the surface of an agar medium. However, this loop-shaped part
Even in the case of using a platinum loop having the same properties, it is unavoidable to damage the surface of the culture medium when applying it to the surface.
この発明は、以上の通りの事情に鴛みてなされたもので
あり、従来の白金耳、さらには木製つまようじ等の植菌
用具とこれを用いた植菌方法の欠点を克服し、培地表面
を損傷することがなく、コロニー数の多い植菌を可能と
する新しい植菌用具とこれを用いた植菌方法とを提供す
ることを目的としている。This invention was made in consideration of the above-mentioned circumstances, and it overcomes the drawbacks of conventional inoculation tools such as platinum loops and wooden toothpicks, and the inoculation methods using these, and damages the surface of the culture medium. The purpose of the present invention is to provide a new inoculation tool and an inoculation method using the same, which enable inoculation with a large number of colonies without the need for inoculation.
（課題を解決するための手段）
この発明は、上記の課題を解決するために、繊維を支持
体の先端部に装着してなることを特徴とする植菌用具を
提供する。(Means for Solving the Problems) In order to solve the above problems, the present invention provides an inoculation tool characterized by having fibers attached to the tip of a support.
この繊維を装着した植菌用具としては、綿等の天然繊維
、合成繊維を、木、竹、合成樹脂、金属等の棒状の支持
体の先端部に巻付けたものや、接着剤等によって固定し
たもの、あるいはそれを着脱自在としたものなどを例示
することができる。Inoculation tools equipped with this fiber include natural fibers such as cotton or synthetic fibers wrapped around the tip of a rod-shaped support made of wood, bamboo, synthetic resin, metal, etc., or fixed with adhesive, etc. For example, it can be attached or detached.
この植菌用具は、従来の方法と同様に培地表面に塗布植
菌する場合に使用するが、この際に、シャーレの培地表
面を下方に向けて植菌するとさらに培地の損傷を防止す
るのに効果的である。This inoculation tool is used to apply and inoculate the surface of the culture medium in the same way as in the conventional method, but at this time, inoculating with the surface of the culture medium in the petri dish facing downward will further prevent damage to the culture medium. Effective.
（作 用）
この発明の植菌用具は、支持体の先端にｍ維を装着して
いるので、従来の白金耳、木製つまようじのように硬質
先端を持たないため、ソフトタッチで培地表面に植菌す
ることができる。しかも培地表面を下方に向けた状態と
すると、さらに培地表面と繊維との接触に不要な力が加
わらない、このため、培地表面を損傷することはなく、
コロニーの分離は良好であって、数多くのコロニーの生
成が可能となる。(Function) The inoculation tool of the present invention has M fibers attached to the tip of the support, so it does not have a hard tip like conventional platinum loops or wooden toothpicks, so it can be used to inoculate the culture medium surface with a soft touch. Can be bacterium. Moreover, when the culture medium surface is directed downward, unnecessary force is not applied to the contact between the culture medium surface and the fibers, and therefore the culture medium surface is not damaged.
Colony separation is good and large numbers of colonies can be produced.
（実施例）
この発明の植菌用具の一例を示したものが第１図である
。直径２〜４ｆｉ、長さ１０〜２０３の竹製、樹脂製、
金属製の棒体（１）の先端に、脱脂綿（２）を装着して
いる。この脱脂綿（２）の大きさは、たとえば、その径
を約２〜６ｆｉ程度としている。(Example) FIG. 1 shows an example of the inoculation tool of the present invention. Made of bamboo, resin, with a diameter of 2 to 4 fi and a length of 10 to 203 mm.
Absorbent cotton (2) is attached to the tip of a metal rod (1). The size of this absorbent cotton (2) is, for example, approximately 2 to 6 fi in diameter.
この植菌用具を用いて以下の植菌を行った。The following inoculation was performed using this inoculation tool.
すなわち、まず、シャーレを上下逆に机上に置き、塁を
そのままにして寒天の入ったシャーレを左手指先で持ち
上げる。That is, first, place the petri dish upside down on the desk, leave the base as it is, and lift the petri dish containing agar with the fingertips of your left hand.
寒天面をほぼ自分の顔に向ける位置に保ちながらサルモ
ネラ菌（Ｓ、＋ １１０３）を取った上記の綿棒植菌具
の先端を寒天培地（２％寒天、０．５％イーストエキス
トラクト）表面の隅に数回往復させて菌を塗付は希釈す
る。While keeping the agar side almost facing your face, place the tip of the cotton swab inoculator used to remove Salmonella (S, +1103) into a corner of the surface of the agar medium (2% agar, 0.5% yeast extract). Apply the bacteria several times to dilute it.
綿棒植菌具をかえ、植菌具をすばやく等速度で左右に動
かして培地全体に菌を塗り広げてゆく。Change the cotton swab inoculation tool and quickly move the inoculation tool from side to side at the same speed to spread the bacteria over the entire medium.
１０枚のプレートに菌を広げ一夜３７℃で静置した時に
シングルコロニーが良好に生成していたプレートの枚数
を数えた。その結果、実験者３名の各々について表１に
示したように、６枚、８枚、１０枚であった。The bacteria were spread on 10 plates and allowed to stand overnight at 37°C, and the number of plates on which single colonies were well produced was counted. As a result, as shown in Table 1 for each of the three experimenters, the number of sheets was 6, 8, and 10.
比較のために同様の操作を従来の植菌具である白金耳を
用いて行ったところ、各々、１枚、５枚、９枚であった
。For comparison, similar operations were performed using platinum loops, which are a conventional inoculation tool, and the results were 1, 5, and 9, respectively.
この結果から明らかなように、この発明の植菌用具を用
いることにより、シングルコロニーの生成は極めて良好
となる。As is clear from this result, by using the inoculation tool of the present invention, the generation of single colonies is extremely good.
もちろんこの発明は、以上の例に限定されるものではな
い、植菌用具の素材、形状などの細部については様々な
態様が可能であり、また培地の種類、植菌の方式につい
ても格別の限定はない、また、植菌処理についても適宜
に選択できることはいうまでもない。Of course, this invention is not limited to the above examples; various details such as the material and shape of the inoculation tool are possible, and there are no particular limitations on the type of culture medium or the inoculation method. It goes without saying that the inoculation treatment can be selected as appropriate.
（発明の効果）
この発明により、以上詳しく説明した通り、培地表面の
損傷を大幅に低減し、コロニーの生成個数の多い植菌が
可能となる。(Effects of the Invention) As explained in detail above, the present invention enables inoculation that greatly reduces damage to the culture medium surface and produces a large number of colonies.
また、この植菌具については製造、取扱いも極めて容易
である。Furthermore, this inoculation tool is extremely easy to manufacture and handle.
第１図は、この発明の植菌具の一例を示した斜視図であ
る。また第２図は、従来の白金耳の例を示した斜視図で
ある。
１・・・棒 体
２・・・脱脂綿FIG. 1 is a perspective view showing an example of the inoculation tool of the present invention. Moreover, FIG. 2 is a perspective view showing an example of a conventional platinum loop. 1... Stick Body 2... Absorbent cotton
Claims (2)
する植菌用具。(1) An inoculation tool characterized by having fibers attached to the tip of a support.
布植菌することを特徴とする植菌方法。(2) A method of inoculation, which comprises applying and inoculating the surface of a culture medium using the inoculation tool according to claim (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17589288A JPH0227975A (en) | 1988-07-14 | 1988-07-14 | Tool for fungus inoculation and fungus inoculation using same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17589288A JPH0227975A (en) | 1988-07-14 | 1988-07-14 | Tool for fungus inoculation and fungus inoculation using same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0227975A true JPH0227975A (en) | 1990-01-30 |
Family
ID=16004052
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17589288A Pending JPH0227975A (en) | 1988-07-14 | 1988-07-14 | Tool for fungus inoculation and fungus inoculation using same |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0227975A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007518408A (en) * | 2004-01-22 | 2007-07-12 | メドベット サイエンス ピーティーワイ リミテッド | Microbial drawing device |
-
1988
- 1988-07-14 JP JP17589288A patent/JPH0227975A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007518408A (en) * | 2004-01-22 | 2007-07-12 | メドベット サイエンス ピーティーワイ リミテッド | Microbial drawing device |
| US7863034B2 (en) | 2004-01-22 | 2011-01-04 | Medvet Science Pty Ltd | Microbial streaking device |
| JP4806637B2 (en) * | 2004-01-22 | 2011-11-02 | メドベット サイエンス ピーティーワイ リミテッド | Microbial drawing device |
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