JPH02203255A - Gel electrophoresis apparatus - Google Patents
Gel electrophoresis apparatusInfo
- Publication number
- JPH02203255A JPH02203255A JP8923593A JP2359389A JPH02203255A JP H02203255 A JPH02203255 A JP H02203255A JP 8923593 A JP8923593 A JP 8923593A JP 2359389 A JP2359389 A JP 2359389A JP H02203255 A JPH02203255 A JP H02203255A
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- excitation light
- excitation
- gel
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001502 gel electrophoresis Methods 0.000 title claims description 9
- 230000003287 optical effect Effects 0.000 claims abstract description 12
- 238000013508 migration Methods 0.000 claims abstract description 11
- 230000005012 migration Effects 0.000 claims abstract description 11
- 230000005284 excitation Effects 0.000 claims description 42
- 239000000126 substance Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 4
- 150000007523 nucleic acids Chemical group 0.000 claims description 3
- 238000005375 photometry Methods 0.000 claims description 2
- 239000012634 fragment Substances 0.000 abstract description 10
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 abstract description 7
- JGVWCANSWKRBCS-UHFFFAOYSA-N tetramethylrhodamine thiocyanate Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=C(SC#N)C=C1C(O)=O JGVWCANSWKRBCS-UHFFFAOYSA-N 0.000 abstract description 6
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 abstract description 4
- 238000005259 measurement Methods 0.000 abstract description 2
- 230000004936 stimulating effect Effects 0.000 abstract 4
- 230000001678 irradiating effect Effects 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 description 13
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 8
- 229910052786 argon Inorganic materials 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 240000000491 Corchorus aestuans Species 0.000 description 1
- 235000011777 Corchorus aestuans Nutrition 0.000 description 1
- 235000010862 Corchorus capsularis Nutrition 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000005297 pyrex Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は蛍光を用いた塩基配列決定装置で用いられるゲ
ル電気泳動装置に関し、さらに具体的には蛍光物質でラ
ベルされた核酸断片試料をゲル電気泳動法により複数・
のレーンで同時に泳動させる泳動ゲルに対し、レーンに
直角方向に機械的に走査される励起・検出系を備え、泳
動途中で蛍光検出するゲル電気泳動装置に関するもので
ある。DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a gel electrophoresis device used in a base sequencing device using fluorescence, and more specifically, the present invention relates to a gel electrophoresis device used in a base sequencing device using fluorescence. By electrophoresis method, multiple
This invention relates to a gel electrophoresis device that is equipped with an excitation/detection system that mechanically scans a migration gel in a direction perpendicular to the lanes, and detects fluorescence during migration.
（従来の技術）
蛍光検出型ゲル電気泳動装置の一例は、第３図に示され
るものである（ｒＨｉｇｈ Ｔｅｃｈｎｏｌｏｇｙ４誌
、１９８６年１２月号、４６−５０ページ参照）。(Prior Art) An example of a fluorescence detection type gel electrophoresis device is shown in FIG. 3 (see rHigh Technology 4, December 1986 issue, pages 46-50).
サンプルは４種の蛍光色素に染め分けられて、混合状態
で、泳動ゲル７ｏの上端から複数のレーンに沿って同時
に泳動される。泳動途中でＤＮＡ断片を検出するために
、励起光光源として多波長同時発振のレーザ７２が用い
られており、レーザ７２からの特定の一波長がフィルタ
で切り換えられて泳動ゲル７０に照射される。泳動ゲル
７０を泳動中のＤＮＡ断片の各色素からの蛍光は、それ
ぞれ透過波長の異なる干渉フィルタ７４が切り換えられ
ることによって１個の光電子増倍管７６により時間分割
で測定される。励起光側のフィルタの切換えと受光側の
干渉フィルタ７４の切換えは同期して行なわれる。複数
のレーンを泳動中のＤＮＡ断片を検出するために、励起
光による照射位置は、励起光束の走査によりレーンと直
角方向に移動させられる。The sample is dyed with four types of fluorescent dyes, and in a mixed state is simultaneously migrated along a plurality of lanes from the upper end of the electrophoresis gel 7o. In order to detect DNA fragments during electrophoresis, a multi-wavelength simultaneous oscillation laser 72 is used as an excitation light source, and one specific wavelength from the laser 72 is switched by a filter and irradiated onto the electrophoresis gel 70. The fluorescence from each dye of the DNA fragment being electrophoresed in the electrophoresis gel 70 is measured in a time-divided manner by one photomultiplier tube 76 by switching the interference filters 74 having different transmission wavelengths. Switching of the filter on the excitation light side and switching of the interference filter 74 on the light receiving side are performed synchronously. In order to detect DNA fragments migrating in a plurality of lanes, the irradiation position with excitation light is moved in a direction perpendicular to the lanes by scanning the excitation light beam.
（発明が解決しようとする課題）
第３図の装置では、１個の光電子増倍管７６によって一
時に検出される螢光物質は４種類のうちのいずれか１つ
である。４種類の螢光物質が用いられるので、各螢光物
質のジューティ・ファクタは１／４となって信号の利用
率が低くなり、測定時間当たりのＳ／Ｎが悪くなる欠点
がある。(Problems to be Solved by the Invention) In the apparatus shown in FIG. 3, one of four types of fluorescent substances is detected at a time by one photomultiplier tube 76. Since four types of fluorescent substances are used, the jute factor of each fluorescent substance is 1/4, resulting in a low signal utilization rate and a disadvantage that the S/N per measurement time is poor.
本発明は複数のレーンに渡って４種類の螢光物質でラベ
ルされた核酸断片試料が混合状態で泳動される装置にお
いて、信号の利用率を上げてＳ／Ｎを高めることを目的
とするものである。The purpose of the present invention is to increase the signal utilization rate and increase the S/N ratio in an apparatus in which nucleic acid fragment samples labeled with four types of fluorescent substances are electrophoresed in a mixed state across multiple lanes. It is.
（課題を解決するための手段）
本発明では、励起光光源からの光を波長により分光し、
分光された６光を励起光として泳動ゲル上で走査方向に
沿った複数の場所を照射し、各励起光による蛍光をそれ
ぞれ独立した蛍光測光部で受光する。(Means for Solving the Problems) In the present invention, light from an excitation light source is separated into wavelengths,
The six separated lights are used as excitation light to irradiate a plurality of locations on the migration gel along the scanning direction, and the fluorescence from each excitation light is received by an independent fluorescence photometer.
（作用）
励起光光源からの光を分光し、分光後の光をそれぞれ励
起光とするとともに、各励起光により核酸断片から発生
した蛍光は、各励起光ごとに独立した蛍光測光部で受光
されるため、励起光をフィルタにより切り換えることが
避けられ、励起光の利用率が上がる。(Function) The light from the excitation light source is separated, and each light after the separation is used as excitation light, and the fluorescence generated from the nucleic acid fragments by each excitation light is received by an independent fluorescence photometer for each excitation light. Therefore, switching the excitation light using a filter can be avoided, and the utilization rate of the excitation light can be increased.
また、泳動ゲル上の励起光照射される複数の位置が泳動
ゲル上で走査方向の直線上に並んでいるので、８１！定
場所は励起波長によらず一定となる。In addition, since the plurality of positions on the electrophoresis gel that are irradiated with excitation light are lined up on a straight line in the scanning direction on the electrophoresis gel, 81! The fixed location remains constant regardless of the excitation wavelength.
（実施例）
第１図は一実施例において、励起・検出系の走査方向に
直交する方向から見た光学系を示す図である。(Example) FIG. 1 is a diagram showing an optical system in an example as seen from a direction perpendicular to the scanning direction of the excitation/detection system.
２はポリアクリルアミドの泳動ゲルであり、走査方向に
切断された状態が示されている。泳動ゲル２はパイレッ
クスガラスにてなる泳動板２．６により挾まれて保持さ
れている。2 is a polyacrylamide migration gel, which is shown cut in the scanning direction. The electrophoresis gel 2 is held between electrophoresis plates 2.6 made of Pyrex glass.
８は励起光光源であるアルゴンレーザ、１２はレーザビ
ーム１０を泳動ゲル２の面に垂直な方向に向けるミラー
である。レーザビーム１０は同時発振された４８８ｎｍ
と５１４ｎｍの光を含んでいる。１４はミラー１２で反
射されたレーザビーム１０の光路に傾斜して置かれたダ
イクロイックミラーであり、５００ｎｍ以上の波長の光
を反射し、それ未満の波長の光を通過させる特性をもっ
ている。したがって、アルゴンレーザビーム１０の内、
４８８ｎｍの光を通過させて直進させ、５１４ｎｍの光
を反射させる。8 is an argon laser which is an excitation light source, and 12 is a mirror that directs the laser beam 10 in a direction perpendicular to the surface of the migration gel 2. Laser beam 10 is simultaneously oscillated at 488 nm.
and 514 nm light. A dichroic mirror 14 is placed obliquely in the optical path of the laser beam 10 reflected by the mirror 12, and has a characteristic of reflecting light with a wavelength of 500 nm or more and allowing light with a wavelength shorter than 500 nm to pass. Therefore, of the argon laser beam 10,
Light of 488 nm is passed through and goes straight, and light of 514 nm is reflected.
ミラー１６、レンズ１８及びミラー２０はダイクロイッ
クミラー１４を通過した波長４８８ｎｍの励起光１０ａ
を集光し、泳動ゲル２に照射する光学系である。レンズ
２２及びミラー２４はダイクロイックミラー１４で反射
された通過した波長５１４ｎｍの励起光１０ｂを集光し
、泳動ゲル２に照射する光学系である。励起光１０ａ、
１０ｂでそれぞれ照射された泳動ゲル上の位置にＤＮＡ
断片が泳動されてきておれば、そのＤＮＡ断片をラベル
している螢光物質から蛍光が発生するゆレンズ２６は励
起′光１０ａで照射された位置からの蛍光２５を集め、
平行光線とするレンズであり、２８は平行化された蛍光
を干渉フィルタ３０又は３２に入射させるミラーである
。干渉フィルタ３０は５２０ｎｍの波長を通過させる特
性をもち、干渉フィルタ３２は５５０ｎｍの波長を通過
させる特性をもっている。干渉フィルタ３０と干渉フィ
ルタ３２はフィルタ回転板３４に取りつけられ、フィル
タ回転板３４の回転しこより蛍光２５の入射位置に切り
換えられて配置される。３６は光電子増倍管、３８は干
渉フィルタ３０又は３２を通過した蛍光を光電子増倍管
３６に導くレンズである。レンズ４２は励起光１０ｂで
照射された位置からの蛍光４０を集め、平行光線とする
レンズであり、４４は平行化された蛍光を干渉フィルタ
４６又は４８に入射させるミラーである。干渉フィルタ
４６は５８０ｎｍの波長を通過させる特性をもち、干渉
フ、イルタ４８は６１０ｎｍの波長を通過させる特性を
もっている。干渉フィルタ４６と干渉フィルタ４８はフ
ィルタ回転板５０に取りつけられ、フィルタ回転板５０
の回転により蛍光４０の入射位置に切り換えられて配置
される。The mirror 16, the lens 18, and the mirror 20 receive the excitation light 10a with a wavelength of 488 nm that has passed through the dichroic mirror 14.
This is an optical system that collects light and irradiates it onto the electrophoresis gel 2. The lens 22 and the mirror 24 are an optical system that collects the excitation light 10b having a wavelength of 514 nm that has been reflected by the dichroic mirror 14 and irradiates it onto the migration gel 2. Excitation light 10a,
10b at each position on the irradiated electrophoresis gel.
If the fragment has been electrophoresed, the lens 26, which generates fluorescence from the fluorescent substance that labels the DNA fragment, collects the fluorescence 25 from the position irradiated with the excitation light 10a.
It is a lens that makes parallel light rays, and 28 is a mirror that makes the collimated fluorescence enter an interference filter 30 or 32. The interference filter 30 has a characteristic of passing a wavelength of 520 nm, and the interference filter 32 has a characteristic of passing a wavelength of 550 nm. The interference filter 30 and the interference filter 32 are attached to a filter rotary plate 34, and are arranged so as to be switched to the incident position of the fluorescent light 25 through the rotation of the filter rotary plate 34. 36 is a photomultiplier tube, and 38 is a lens that guides the fluorescence that has passed through the interference filter 30 or 32 to the photomultiplier tube 36. The lens 42 is a lens that collects the fluorescent light 40 from the position irradiated with the excitation light 10b and converts it into a parallel beam, and 44 is a mirror that makes the collimated fluorescent light enter an interference filter 46 or 48. The interference filter 46 has a characteristic of passing a wavelength of 580 nm, and the interference filter 48 has a characteristic of passing a wavelength of 610 nm. The interference filter 46 and the interference filter 48 are attached to a filter rotating plate 50, and the filter rotating plate 50
The incident position of the fluorescent light 40 is switched and arranged by the rotation of the fluorescent light 40 .
５２は光電子増倍管、５４は干渉フィルタ４６又は４８
を通過した蛍光を光電子増倍管５２に導くレンズである
。52 is a photomultiplier tube, 54 is an interference filter 46 or 48
This lens guides the fluorescent light that has passed through the photomultiplier tube 52.
破線で囲まれた領域内にある光学系が一体としてＸ及び
−Ｘ方向に機械的に走査される。この光学系の走査とフ
ィルタ回転板３４．５０の回転が連動してなされる。す
なわち、走査がＸ方向に行なわれるときは干渉フィルタ
３０と干渉フィルタ４６が蛍光受光位置にセットされ、
走査が−Ｘ方向に行なわれるときは干渉フィルタ３２と
干渉フィルタ４８が蛍光受光位置にセットされる。The optical system within the area surrounded by the broken line is mechanically scanned as a unit in the X and -X directions. The scanning of this optical system and the rotation of the filter rotary plate 34,50 are performed in conjunction with each other. That is, when scanning is performed in the X direction, the interference filter 30 and the interference filter 46 are set at the fluorescence receiving position,
When scanning is performed in the -X direction, the interference filter 32 and the interference filter 48 are set at the fluorescence receiving position.
次に、本実施例の動作について説明する。Next, the operation of this embodiment will be explained.
泳動ゲル２中を泳動するＤＮＡ断片をラベルしている螢
光物質は、ＦＩＴＣ，ＮＢＤ、ＴＲ４ＴＣ、Ｔｅｘａｓ
Ｒｅｄの４種類であり、末端塩基別に染め分けている
。ＦＩＴＣはアルゴンレーザビームの４８８ｎｍで励起
され、５２０ｎｍにピークをもつ蛍光を発する。ＮＢＤ
は４８８ｎｍで励起され、５５０ｎｍにピークをもつ蛍
光を発する。ＴＲＩＴＣは５１４ｎｍで励起され、５８
０ｎｍにピークをもつ蛍光を発するｓ Ｔｅｘａｓ Ｒ
ｅｄは５１４ｎｍで励起され、６１０ｎｍにピークをも
つ蛍光を発する。The fluorescent substances that label the DNA fragments running in the electrophoresis gel 2 are FITC, NBD, TR4TC, and Texas.
There are four types of red, and they are dyed differently depending on the terminal base. FITC is excited by an argon laser beam at 488 nm and emits fluorescence with a peak at 520 nm. NBD
is excited at 488 nm and emits fluorescence with a peak at 550 nm. TRITC is excited at 514 nm and 58
Texas R emits fluorescence with a peak at 0 nm
ed is excited at 514 nm and emits fluorescence with a peak at 610 nm.
ダイクロイックミラー１４を通過した波長４８８、ｎｍ
の励起光１０ａの照射によって励起される螢光物質はＦ
ＩＴＣとＮＢＤであり、ダイクロイックミラー１４で反
射された波長５１４ｎｍの励起光１０ｂの照射によって
励起される螢光物質はＴＲＩＴＣとＴｅｘａｓ Ｒｅｄ
である。励起光１０ａで励起されて発生する蛍光２５の
うち、５２０’ ｎ ｍを通す干渉フィルタ３０を通過
して光電子増倍管３６で検出される蛍光はＦＩＴＣから
の蛍光であり、５５０ｎｍを通す干渉フィルタ３２を通
過して光電子増倍管３６で検出される蛍光はＮＢＤから
の蛍光である。また、励起光１０ｂで励起されて発生す
る蛍光４０のうち、５８０ｎｍを通す干渉フィルタ４６
を通過して光電子増倍管５２で検出される蛍光はＴＲＩ
ＴＣからの蛍光であり、６１０ｎｍを通す干渉フィルタ
４８を通過して光電子増倍管５２で検出される蛍光はＴ
ｅｘａｓ Ｒｅｄからの蛍光である。Wavelength 488 nm passed through dichroic mirror 14
The fluorescent substance excited by the irradiation with the excitation light 10a is F
The fluorescent substances excited by the irradiation of the excitation light 10b with a wavelength of 514 nm reflected by the dichroic mirror 14 are TRITC and Texas Red.
It is. Of the fluorescence 25 generated by being excited by the excitation light 10a, the fluorescence that passes through the interference filter 30 that passes 520' nm and is detected by the photomultiplier tube 36 is fluorescence from FITC, and is the fluorescence that passes through the interference filter 30 that passes 520' nm. The fluorescence that passes through the photomultiplier tube 32 and is detected by the photomultiplier tube 36 is the fluorescence from the NBD. Also, an interference filter 46 that passes 580 nm of the fluorescence 40 generated by being excited by the excitation light 10b.
The fluorescence that passes through and is detected by the photomultiplier tube 52 is TRI.
The fluorescence from TC, which passes through the interference filter 48 that passes 610 nm and is detected by the photomultiplier tube 52, is T.
Fluorescence from exas Red.
鎖線で囲まれた光学系がＸ方向に機械的に走査されると
き、干渉フィルタは５２０ｎｍの干渉フィルタ３ｏと５
８０ｎｍの干渉フィルタ４６が蛍光受光位置にセットさ
れているので、励起光１０ａ、１０ｂで照射される位置
にＦＩＴＣ又はＴＲＩＴＣでラベルされたＤＮＡ断片が
存在しておれば、ＦＩＴＣからの蛍光は光電子増倍管３
６で検出され、ＴＲＩＴＣからの蛍光は光電子増倍管５
２で検出される。光学系が−Ｘ方向に機械的に走査され
るとき、干渉フィルタは５５０ｎｍの干渉フィルタ３２
と６１０ｎｍの干渉フィルタ４８が蛍光受光位置にセッ
トされているので、励起光１０ａ、１０ｂで照射される
位置にＮＢＤ又はＴａｘａｓ ＲｅｄでラベルされたＤ
ＮＡ断片が存在しておれば、ＮＢＤからの蛍光は光電子
増倍管３６で検出され、Ｔｅｘａｓ Ｒｅｄからの蛍光
は光電子増倍管５２で検出される。When the optical system surrounded by the chain line is mechanically scanned in the X direction, the interference filters are 520 nm interference filters 3o and 5.
Since the 80 nm interference filter 46 is set at the fluorescence receiving position, if a DNA fragment labeled with FITC or TRITC exists at the position irradiated with the excitation light 10a, 10b, the fluorescence from FITC will be photoelectron-intensified. Double tube 3
6, and the fluorescence from TRITC is detected by photomultiplier tube 5.
Detected at 2. When the optical system is mechanically scanned in the -X direction, the interference filter is a 550 nm interference filter 32.
Since the 610 nm interference filter 48 is set at the fluorescence receiving position, the D labeled with NBD or Taxas Red is placed at the position irradiated with the excitation light 10a and 10b.
If NA fragments are present, fluorescence from NBD is detected by photomultiplier tube 36 and fluorescence from Texas Red is detected by photomultiplier tube 52.
第１図の実施例では、各励起光１０ａ、１０ｂで励起さ
れて発生したそれぞれ２種類の蛍光を、励起光ごとに独
立したそれぞれの光電子増倍管３６．５２で検出するた
めに、干渉フィルタ３０と３２．４６と４８を切り換え
ているが、このように干渉フィルタを切り換えるのに代
えて、第２図に示されるように、ダイクロイックミラー
５６を用い、ダイクロイックミラー５６で波長別に分光
された蛍光をそれぞれ別々の光電子増倍管５８゜６０で
検出することもできる。例えば、干渉フィルタ３０．３
２の切換え機構の部分に代わるものとしては、ダイクロ
イックミラー５６として５２０ｎｍの光を通過させ、５
５０ｎｍの光を反射させる特性のものを用いることがで
きる。３０，３２は第１図と同じ干渉フィルタ、３８．
３９は集光レンズである。第１図における干渉フィルタ
４６．４８の切換え機構の部分についても、第２図と同
様の構成に置き換えることができる。ただしダイクロイ
ックミラーの波長特性は異なったものとなる。In the embodiment shown in FIG. 1, interference filters are used to detect two types of fluorescence generated by excitation with each of the excitation lights 10a and 10b using independent photomultiplier tubes 36 and 52 for each excitation light. 30, 32, 46, and 48, but instead of switching the interference filters in this way, a dichroic mirror 56 is used as shown in FIG. They can also be detected using separate photomultiplier tubes 58 and 60. For example, interference filter 30.3
As an alternative to the switching mechanism part 2, a dichroic mirror 56 that passes 520 nm light,
A material having a characteristic of reflecting light of 50 nm can be used. 30, 32 are the same interference filters as in FIG. 1; 38.
39 is a condensing lens. The switching mechanism portion of the interference filters 46 and 48 in FIG. 1 can also be replaced with the same configuration as in FIG. 2. However, the wavelength characteristics of the dichroic mirror will be different.
（発明の効果）
本発明では励起光光源からの複数の波長の光を励起光と
して同時に泳動ゲルに照射し、各照射位置からの蛍光を
別々の蛍光測光部で受光するようにしたので、従来のよ
うに一度には１波長の励起光しか照射しないものに比較
すると２倍の信号を得ることができるようになり、測定
時間当たりのＳ／Ｎが向上する。(Effects of the Invention) In the present invention, the electrophoresis gel is simultaneously irradiated with light of a plurality of wavelengths from an excitation light source as excitation light, and the fluorescence from each irradiation position is received by a separate fluorescence photometry unit. Compared to a method in which only one wavelength of excitation light is irradiated at a time, as shown in FIG.
第１図は一実施例における光学系を示す平面図、第２図
は他の実施例における蛍光受光部を示す平面図、第３図
は従来のゲル電気泳動袋でを示す斜視図である。
２・・・・・・泳動ゲル、８・・・・・・アルゴンレー
ザ、１０・・・・・レーザビーム、１０ａ、１０ｂ・・
・・・・励起光、１４・・・・・・ダイクロイックミラ
ー、３０，３２，４６．４８・・・・・・干渉フィルタ
、３６．５２・・・・・・光電子増倍管。FIG. 1 is a plan view showing an optical system in one embodiment, FIG. 2 is a plan view showing a fluorescent light receiving section in another embodiment, and FIG. 3 is a perspective view showing a conventional gel electrophoresis bag. 2...Migration gel, 8...Argon laser, 10...Laser beam, 10a, 10b...
...Excitation light, 14...Dichroic mirror, 30, 32, 46.48...Interference filter, 36.52...Photomultiplier tube.
Claims (1)
泳動法により複数のレーンで同時に泳動させる泳動ゲル
に対し、レーンに直角方向に機械的に走査される励起・
検出系を備え、泳動途中で蛍光検出するゲル電気泳動装
置において、前記励起・検出系は、励起光光源からの光
を波長により分光し、分光された各光を励起光として泳
動ゲル上で走査方向に沿った複数の場所を照射する励起
光学系と、各励起光による蛍光をそれぞれ受光する蛍光
測光部とを備えていることを特徴とするゲル電気泳動装
置。(1) An excitation beam that is mechanically scanned in a direction perpendicular to the lanes on a migration gel in which nucleic acid fragment samples labeled with a fluorescent substance are simultaneously migrated in multiple lanes using gel electrophoresis.
In a gel electrophoresis apparatus that is equipped with a detection system and detects fluorescence during migration, the excitation/detection system separates light from an excitation light source into wavelengths, and scans the separated lights on the migration gel as excitation light. A gel electrophoresis device comprising: an excitation optical system that irradiates a plurality of locations along a direction; and a fluorescence photometry unit that receives fluorescence from each excitation light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1023593A JP2701412B2 (en) | 1989-01-31 | 1989-01-31 | Gel electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1023593A JP2701412B2 (en) | 1989-01-31 | 1989-01-31 | Gel electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02203255A true JPH02203255A (en) | 1990-08-13 |
| JP2701412B2 JP2701412B2 (en) | 1998-01-21 |
Family
ID=12114882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1023593A Expired - Fee Related JP2701412B2 (en) | 1989-01-31 | 1989-01-31 | Gel electrophoresis device |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP2701412B2 (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04204151A (en) * | 1990-11-30 | 1992-07-24 | Hitachi Software Eng Co Ltd | Polychromatic-migration-pattern reading apparatus |
| JPH04294257A (en) * | 1991-03-22 | 1992-10-19 | Hitachi Software Eng Co Ltd | Multi color migration pattern reader |
| US5290419A (en) * | 1992-04-14 | 1994-03-01 | Hitachi, Ltd. | Fluorescence detection type electrophoresis apparatus |
| JP2001074656A (en) * | 1999-09-03 | 2001-03-23 | Fuji Photo Film Co Ltd | Image data reading apparatus |
| WO2007091530A1 (en) * | 2006-02-07 | 2007-08-16 | The Furukawa Electric Co., Ltd. | Photodetector and measurement object reader |
| WO2011114578A1 (en) * | 2010-03-19 | 2011-09-22 | シャープ株式会社 | Measurement device, measurement method, measurement result processing device, measurement system, measurement result processing method, control program, and recording medium |
| US9060687B2 (en) | 2009-10-02 | 2015-06-23 | Sharp Kabushiki Kaisha | Device for monitoring blood vessel conditions and method for monitoring same |
-
1989
- 1989-01-31 JP JP1023593A patent/JP2701412B2/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04204151A (en) * | 1990-11-30 | 1992-07-24 | Hitachi Software Eng Co Ltd | Polychromatic-migration-pattern reading apparatus |
| US5213673A (en) * | 1990-11-30 | 1993-05-25 | Hitachi Software Engineering Co., Ltd. | Multi-colored electrophoresis pattern reading apparatus |
| JPH04294257A (en) * | 1991-03-22 | 1992-10-19 | Hitachi Software Eng Co Ltd | Multi color migration pattern reader |
| US5190632A (en) * | 1991-03-22 | 1993-03-02 | Hitachi Software Engineering Co., Ltd. | Multi-colored electrophoresis pattern reading system |
| US5290419A (en) * | 1992-04-14 | 1994-03-01 | Hitachi, Ltd. | Fluorescence detection type electrophoresis apparatus |
| JP2001074656A (en) * | 1999-09-03 | 2001-03-23 | Fuji Photo Film Co Ltd | Image data reading apparatus |
| WO2007091530A1 (en) * | 2006-02-07 | 2007-08-16 | The Furukawa Electric Co., Ltd. | Photodetector and measurement object reader |
| US8685710B2 (en) | 2006-02-07 | 2014-04-01 | The Furukawa Electric Co., Ltd. | Photodetector and measurement object reader |
| US9060687B2 (en) | 2009-10-02 | 2015-06-23 | Sharp Kabushiki Kaisha | Device for monitoring blood vessel conditions and method for monitoring same |
| WO2011114578A1 (en) * | 2010-03-19 | 2011-09-22 | シャープ株式会社 | Measurement device, measurement method, measurement result processing device, measurement system, measurement result processing method, control program, and recording medium |
| US9173604B2 (en) | 2010-03-19 | 2015-11-03 | Sharp Kabushiki Kaisha | Measurement device, measurement method, measurement result processing device, measurement system, measurement result processing method, control program, and recording medium |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2701412B2 (en) | 1998-01-21 |
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