JPH02191593A - Reactor for immobilized physiologically active material - Google Patents

Abstract

PURPOSE:To facilitate the dissolution of oxygen and to prevent peeling of physiologically active materials by disposing reaction containers having overflow holes through which liquid and gas can move into multiple stages and providing porous bodies on the contact boundaries of the water surface and gaseous phase thereof. CONSTITUTION:Plural sets of the assemblies formed by combining the Porous bodies 7 (ceramics, sponge, etc.) which can carry the physiologically active materials (enzyme, bacteria, etc.) and the containers 2 opened on one side are disposed into multiple stages in a treated water tank 1 to constitute the reactor. Overflow holes 3 through which the soln. and/or gas can move between the respective containers are formed on one side of the containers 2 and are so disposed that the open ends of the holes 3 are positioned near the surfaces in the upper part of the above-mentioned porous bodies 7. The contact area of the gaseous phase/liquid phase is widened by the oscillation of the water surfaces at the time of supply of the oxygen-contg. gas and the soln., in this constitution, by which the dissolution of oxygen is accelerated and further the need for force aeration and stirring which are heretofore executed is eliminated.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a bioreactor,! l! +Regarding a reaction device used for cell culture. Among these, it relates to a reaction device that is effective for wastewater treatment.

[Prior Art] Activated sludge method has conventionally been adopted as a method for treating wastewater. However, this method has the following problems. The amount of sludge generated is large and it takes time and effort to process. ■There is a limit to the purification ability of microorganisms, and when overloaded, abnormal growth of filamentous fungi and fungi, aerobic digestion due to excessive exposure, and bulking occur due to the disintegration of microbial flocs, making it impossible to discharge water, and at the same time, it is impossible to recover activity. Requires long hours and high level of management. ■Processing time is long and equipment becomes large. Therefore,
If compressed air is sent to shorten processing time, energy consumption will increase. ■Many factories are concentrated in the suburbs of cities, do not have enough land for wastewater treatment facilities, are economically limited, and have many undeveloped management techniques.

In addition, there is a method called contact oxidation method in which microorganisms are attached to the contact material, but it is difficult to quickly attach microorganisms to the surface of the contact material, and even if it is possible to attach microorganisms, the ability to retain microorganisms is insufficient and it is difficult to adhere to the surface of the contact material. However, there was a drawback that microorganisms were removed.

There are other types such as the sprinkler hearth type, but the structure of the hearth for attaching microorganisms is complicated, large and heavy, making it difficult to manufacture and handle.Furthermore, since it is flat, it is difficult to expand the surface area. There was a problem.

When culturing cells, it is necessary to increase the amount of dissolved oxygen for cell growth. Therefore, stirring the liquid,
Alternatively, it is common to perform aeration, which is the introduction of air bubbles. However, when this aeration and stirring are performed, the cells supported on the carrier are detached. As a result, there was a problem in that cell culture became difficult.

[Problem B to be Solved by the Invention] The present invention provides an immobilized physiologically active substance that allows the reaction of a physiologically active substance to occur efficiently, prevents the peeling of the physiologically active substance due to the supply of oxygen-containing gas, and that is easy to handle. The purpose is to provide a reactor.

[Means for Solving the Problem] The present invention provides a reaction device in which a combination of a porous body (7) capable of supporting a physiologically active substance and a container (2) with one side open is arranged in multiple stages. The containers (2) are formed with overflow holes (3) through which solution and/or gas can move between the containers (2), and at least one opening of the perfusion holes (3) is formed. This is an immobilized physiologically active substance reaction device characterized in that the end is located near the upper surface of the porous body (7).

[Operation of the invention] Physiologically active substances supported on the porous body (7) include enzymes and bacterial cells. Types of enzymes and bacterial cells include those whose growth is promoted by oxygen, that is, It is an aerobic bacterium, and examples include Mycobacterium tuberculosis, Mycobacterium tuberculosis, Bacillus acetate, Mycobacterium gluconsan, and molds.

However, when treating wastewater, there is no need to limit the amount since bacterial cells naturally adhere to the porous body (7).

Porous materials (7) include ceramics, sponge non-woven fabrics, etc. Among them, non-woven fabrics are sheet-like and highly flexible, and can not only be easily processed into a predetermined shape, but also can be disposed of after use by incineration, etc. In addition, hydrophobic fibers are most suitable because they are easy to clean and cause less clogging during use.

When using a nonwoven fabric as the porous body (7), it is preferable to use fibers having a fineness of about 1d to 30d and a fiber length of 20+wm or more. In addition, the basis weight of nonwoven fabric is 20 to 300 g/ln', and the thickness is 1 DIllI to
A 20mm one is suitable. Among these ranges, particularly preferably, the constituent fibers of the nonwoven fabric have a fineness of 1 to 10 d and a fiber length of 201 m or more, and the nonwoven fabric has a basis weight of 20 to 150 m.
g/m' and a thickness of 1 to 101I+lll. These values are appropriate values for retaining physiologically active substances, and at the same time, values that do not impair filtration performance and do not cause clogging.

It should be noted that these nonwoven fabrics may have fineness, fiber length, basis weight, and thickness as described above, and the manufacturing method thereof is not particularly limited, such as dry method, spunbond method, wet method, etc.

No! If hydrophobic fibers such as polyolefin fibers and polyester fibers are used for the a-cloth, the nonwoven fabric can be easily cleaned and clogging during use is reduced.

Also, impregnation with hydrophobic latex is one way to facilitate cleaning of non-ai1 fabrics.

If a porous body (7) with a porosity of 90 to 99% is used, the reaction can be carried out more efficiently.

Here, the porosity is determined by the following formula.

(thickness) x (specific gravity) porosity (%) = X
100 (approximate size) If the porosity derived from this definition exceeds 99%, the microbial trapping effect and the solid matter trapping effect become small. Moreover, if it is less than 90%, the movement of the solution into the inside of the porous body (7) will be insufficient, which is not preferable.

Therefore, the porosity is preferably 90% to 99%.

Moreover, the porous body (7) may be one or two or more stacked. When one porous body 7) is used, a more effective reaction can be achieved by making a difference in the porosity of both surfaces of the porous body (7). In addition, in the case of two or more porous bodies (
7) may have different porosity, but it is better to set a fixed porosity for each porous body (7) and arrange them in order of increasing porosity with respect to □ in the part where the solution penetrates. Even when providing a porosity, the part where the solution enters should have a porosity of about 99%.
The portion where the solution flows out from the porous body (7) preferably has a porosity of 95% or less. This is 99 as above
% or more, the trapping effect becomes smaller.

The outflow portion preferably has a porosity of 95% or less, taking into consideration the movement of the solution.

A container with one side open and having an overflow hole is one in which the hole is simply widened in an n-shape in the vertical cross section, as shown in Figures 2, 4, and 6. Examples include a type having an n-shape and an overflow pipe, and a type having a boat shape and an overflow pipe.

The present invention will be described below with reference to illustrated FIGS. 1 and 2.

The treated water tank (1) accommodates a container (2), a porous body (7), and the like. Therefore, the size of the treated water tank (1),
The shape depends on the size and shape of the container (2) and porous body (7). Change as appropriate.

Container (2) is a container for forming a gas phase;
) to make it easier to dissolve oxygen in the gas phase in the solution and make it easier to react.

As shown in Figure 2, the container (2) is generally considered to have an n-shaped shape with a widening base in the longitudinal section, but any shape that can form a gas phase can be used. The cross section of the container (2) is generally rectangular, but may be circular, and is not particularly limited.

The material is not particularly limited either, but plastic or the like may be used to prevent rust.

It is preferable that the width (A) of the container is approximately the same length as the inner wall of the treated water tank (1) to be used. This is to allow the solution to react efficiently.

The container (2) is provided with an overflow hole (3). This overflow hole (3) is used for the flow of solution and/or gas. The overflow hole (3) can be provided by simply making a hole in the container (2) as shown in FIG. 2, or by using an overflow pipe (9) as shown in FIGS. 4 and 6.

In each stage of containers (2), it is preferable that the overflow holes (3) are arranged at different positions on the slope of the container (2). This is because when the overflow hole (3) is in the same position and oxygen-containing gas is supplied, the gas does not spread into the container (2) and immediately escapes upward, causing the oxygen to dissolve into the solution. This is because the effect will be halved.

In order to increase contact with oxygen, the overflow hole (3) should be placed as far away as possible so that oxygen can dissolve more easily and physiologically active substances can grow more easily.
If the cross section is rectangular, it is effective to install them alternately on opposite sides.

The overflow hole (3) may have any shape, but
Generally circular overflow holes (3) are used.

When the overflow hole (3) is positioned at a height similar to the top of the porous body (7), a gas phase is formed above the bottom end of the downward protrusion, and oxygen-containing gas is supplied. Porous materials (7) because the air in the gas phase cannot be compressed even if
The upper surface of the porous body (7) will be located where the top gas phase and the liquid phase meet, and if oxygen-containing gas is supplied from the air introduction vibrator (6), the water surface will shake due to the supply of oxygen, causing the porous body (7 ) is exposed in the gas phase, □ the porous body (7
) comes into contact with the air more often, promoting the dissolution of oxygen.

The container having only the overflow hole (3) is advantageous in that it is easy to manufacture the container (2) because it is sufficient to simply drill the hole.

Unlike FIG. 1, the oxygen-containing gas may be introduced not only from below the support (8) but also from anywhere. For example, it does not matter at all if it is supplied every -stage. Note that the oxygen-containing gas may be supplied using a vibrator or the like, and is not particularly limited.

The number of sets of porous bodies (7) and containers (2) to be stacked depends on various factors such as processing capacity and space issues, so it may be changed as appropriate.

In addition, these reactors are not only used alone, but also
A plurality of them may be used by arranging and connecting them.

The support (8) that supports the lowermost porous body is the porous body (7)
It is used to fix the container (2), and may be of any shape or material, but like the container (2), it is preferably made of plastic so as not to rust.

In addition, the porous body (7) due to the container (2) and the support (8)
Any method may be used for fixing, but for example, adhesive, rivets, or a method of leaving it still depending on the relationship with specific gravity can be used.

The width of the support is similar to the width of the container (^), and the width of the treated water tank (1)
Preferably, the length is about the same as the inner wall.

The artificial vibe (4) is a vibe for introducing the solution, and the exit vibe (5) is a vibe for discharging the solution after the reaction.

In FIG. 1, the inlet of the inlet vibe (4) is located below the support (8) in order to react over a larger surface area. The solution introduced by this inlet vibrator (4) occupies the space between the support (8) and the treatment water tank (1).

When that space is occupied, it passes through the overflow hole (3),
- The solution that flows in from this overflow hole (3) occupies the space above the step, occupies the space, and compresses the air to a position slightly above the lowest end of the downward protrusion and occupies the space. However, the air cannot be compressed above this position, and the solution flows from the overflow hole (3) into the space above the stage. Similarly, the downward protrusion occupies a space up to a position slightly above the lowest end, and flows into the space of the next stage. (5) The solution after the reaction is drained. Note that when oxygen-containing gas is introduced from the air introduction vibrator (6), the oxygen-containing gas moves into the gas phase formed by the container (2) and flows into it. ii) Since the liquid is compressed, the solution can be circulated more effectively, and oxygen is more easily dissolved in the water. When the oxygen-containing gas reaches the lowest end of the downward protrusion, it passes through the overflow hole (3) and moves to the second stage, producing the same effect.

Also, unlike in Figure 1, if the solution is introduced from a place other than below the bottom of the treated water tank (1), the solution will fall to the bottom step by step, and after occupying the space at the bottom, the solution will enter the treated water tank (1). (
1) As in the case where it is introduced from near the bottom, it occupies the space from the bottom in order, and finally the container (2) of the treated water tank (1)
The part other than the gas phase is filled with the solution, and the porous body (7
) undergoes a reaction due to the physiologically active substance supported on it. The reacted solution is discharged by an exit vibrator (5).

As shown in Section L, the solution may be introduced not only from near the bottom of the treated water tank (1) but also from anywhere.

Similarly, the water may be drained from some place. This introduction and drainage can be done using a vibrator, etc.
It is not particularly limited.

Next, FIGS. 3 and 4 in the case where the overflow pipe (9) is provided will be explained, focusing on the differences from FIG. 1.

In the case of an immobilized physiologically active substance reaction device using an overflow pipe (9) as shown in FIG. 3, the treatment water tank (1) may or may not be provided. This is done by firmly connecting the containers (2) together.
This is because it is unnecessary if it is possible to prevent the solution from leaking.

As the container (2), as shown in FIG. 4, a container having an n-shaped longitudinal section is used. Further, the cross section may be rectangular or circular. In addition, when using the treated water tank (1), it is preferable that the width (A) of the container is approximately the same length as the inner wall of the treated water tank (1) to allow the reaction to occur effectively.

The overflow pipe (9) is provided on the ceiling of the container (2). It is preferable to arrange the overflow pipes (9) at different positions of the containers (2) in each stage. This is because the overflow pipe (9) was in the same position, and when oxygen-containing gas was supplied, the gas did not spread into the container (2) and immediately escaped upwards, causing the oxygen to dissolve into the solution. This is because the effect of doing so will be halved. In order to increase contact with oxygen, the overflow pipe (9) should be installed as far away as possible, so that oxygen can dissolve more easily and physiologically active substances can grow more easily. If the cross section is rectangular, it is effective to install them diagonally.

The overflow pipe (9) may have any shape, but
- A cylindrical overflow pipe (9) is used for boat fishing.

For example, as shown in Figure 3, a porous body (7) is placed inside the container (2).
When the overflow pipe (9) is filled with a top porous body, the sum of the thickness of the porous body (7) and the height (C) of the downward protrusion is about the height of the container ([3]). The lower end of the overflow pipe (9) will reach a position where it will come into contact with the upper surface of (7). When the lower end of the overflow pipe (9) reaches this 112th position, a gas phase is formed above the lowest part of the downward protrusion, and the solution cannot compress the oxygen-containing gas in this gas phase. Therefore, the overflow pipe (9) can adjust the water level to around the surface of the porous body, and if oxygen-containing gas is supplied by the air introduction vibrator (6), the water surface will shake and the porous body will A part of (7) is exposed in the gas phase, and the water adhering to the porous body (7) comes into contact with air more, promoting the dissolution of oxygen.

Note that without putting the porous body (7) into the container (2),
When placed under the container (2), the same effect can be obtained if the height (C) of the downward protrusion is made comparable to the height (B) of the container.

If this overflow pipe (9) has a portion projecting from the ceiling of the container (2), this projecting portion also serves to prevent the porous body (7) from shifting. On the other hand, if there is no protrusion from the ceiling, the porous body (7) can be spread over the ceiling of the container (2), and the porous body (7) needs to be cut to fit the protrusion. Since there is no protrusion, the protrusion may or may not be present.

Next, FIGS. 5 and 6, which are other examples of the apparatus of the present invention, will be described with a focus on their differences.

In the case of an immobilized physiologically active substance reaction device as shown in FIG. 5, a treatment water tank (+) may or may not be provided. Treatment water tank (1)
When using the overflow pipe (9), it is better to provide an air circulation hole (lO) in the container (2) above the upper end of the overflow pipe (9). This is to facilitate the dissolution of oxygen into the water, which tends to be insufficient with the overflow hole (3) alone. In addition, a treated water tank (1
) is not used, a supporting rod, for example, is required between the containers (2).

In the case of the reactor shown in FIG. 5, the container (2) holds the porous body (7) and also controls the water surface level through the overflow pipe (9).

As shown in FIG. 6, the container (2) generally has a boat-shaped longitudinal section and is provided with an overflow pipe (9) in its flat part. Further, the cross section of the container (2) is generally rectangular, but may be circular, and is not particularly limited. The material is not particularly limited either, but plastic or the like may be used to prevent rust.

It is preferable that the overflow pipes (9) attached to the containers (2) be arranged at different positions of the containers (2) in each stage of the containers (2). This is because if the overflow tubes (9) were located at the same position, the solution would directly pass through to the lower stage and would not undergo any reaction by the physiologically active substance.

The overflow pipe (9) may have any shape, but
- A cylindrical overflow pipe (9) is used for boat fishing.

This overflow pipe (9) is installed so as to protrude upward from the bottom of the container to the same extent as J5 of the porous body (7). In addition, since surface tension acts on the residue, the liquid level is usually about 1 to 5 W + W higher than the top of the hot flow tube. Good even without the upward protrusion.

When the overflow pipe (9) is installed, the solution sent from the large donkey eve (4) will fill the porous body (7) to the extent that it is thick, and if more solution is sent in, it will overflow. Since the solution flows down to the lower stage through the pipe (9), the water surface level can be determined near the upper surface of the porous body (7) by means of this overflow pipe (9). In addition to this, the shaking of the water surface as the solution is supplied increases the chances of the solution coming into contact with air, promoting the dissolution of oxygen. The more oxygen is dissolved, the more physiologically active substances become reactive. Note that this overflow pipe (9) serves to fix the porous body (7) by its upwardly protruding portion. Although there is no particular need for the downward protrusion, the downward protrusion can be useful for pressing and fixing the upper surface of the lower porous body (7).

The number of sets of porous bodies (7) and containers (2) to be stacked depends on various factors such as processing capacity and space problems, so it may be changed as appropriate.

In addition, these reactions H@ are not only used alone, but also
? You may use it by arranging and concatenating jJ numbers. Note that the solution drained from the outlet vibrator (5) may be fed back to the inlet vibrator (4) for circulation.

Note that any method may be used to fix the porous body (7) to the container (2), but for example, adhesives, rivets, or a method of leaving it still depending on the relationship with the specific gravity can be used.

As for the flow of the solution, the solution is introduced from the artificial vibrator (4) and grooves are sequentially formed in the porous body (7).

The overflow pipe (9) is filled with the solution up to the upper end of the upper protrusion, and if any more solution is fed, it flows to the lower stage through the i8 flow hole (3) formed by the overflow pipe (9). Fall down.

This flowing solution also occupies the layer of the porous body (7), and when it reaches the upper end of the upper protrusion of the overflow pipe (9), it flows further into the lower stage. By repeating the above steps, when it reaches the stage where the exit vibe (5) is located, it is sent through the exit vibe (5) to the drainage or to the artificial vibe (4) again.

If wastewater is introduced from the inlet vibrator (4) as a solution to be circulated through the reaction apparatus as shown in FIGS. 1, 3, and 5, it can be used as a wastewater treatment apparatus.

Moreover, the apparatuses shown in FIGS. 1, 3, and 5 can be used not only as an apparatus for treating wastewater, but also as an apparatus for cell culture. However, in the case of cell culture, issues generally include pH1 temperature, culture time, and composition of the culture medium.

Among these, the pH 1 culture time and the composition of the culture solution may be changed as appropriate depending on the type of physiologically active substance.

Regarding the temperature, the temperature may be adjusted in the treated water tank (1) and the container (2) themselves, or the culture solution may be supplied after adjusting the temperature, and is not particularly limited.

Further, when culturing cells and using a nonwoven fabric as the porous body (7), the fineness of the constituent fibers is 0.05 to 1.

5d, the porosity is preferably 80 to 50%.

This is because the above-mentioned fineness and porosity match the size of the cells, making it easier to support the cells firmly on the nonwoven fabric.

When used as a bioreactor, pi-1, temperature, time, etc. are issues, as in the case of cell culture. Among these, the p 01 time may be changed as appropriate depending on the type of physiologically active substance.

Regarding the temperature, it is sufficient to adjust the temperature in the treated water tank (1) and container (2) themselves, and there is no particular limitation.

Immobilization methods that can be used when used as this bioreactor include a covalent bond method and an ionic bond method, but the entrapment method is common.

Among the comprehensive methods, the lattice type is mainly used. There are various methods for this lattice type, including those using polyacrylamide gel, natural polymers, radiation polymerization, photocrosslinkable prepolymers, and urethane resin prepolymers.

In addition to the lattice type, microcapsule type, ribosome type, and methods that trap physiologically active substances in the nonwoven fabric itself made of hollow fibers are also used.

In addition to the entrapment method, as a physical adsorption method, stable immobilized physiologically active substances can be obtained by using fibers with active groups such as nylon and selecting a porosity suitable for each physiologically active substance.

As a reaction product using a bioreactor equipped with a porous material fixed with physiologically active substances using the method described above,
L-amino acid, aspartic acid.

Various products such as L-glutamic acid and L-alanine can be obtained.

[Example] (Example 1) Wastewater treatment was carried out using a device as shown in FIG.

The size of the treated water tank (1) is 20cm long and 15cm wide.
, height 50C! It was set to 11. The height of the container made of plastic (B) is 12+++s, the height of the downward protrusion (C)
was set at 6 m. Using nonwoven fabric as the porous body 7),
The non-woven fabric is polypropylene fiber (6ij x 76m
), with a thickness of 611 mm and a basis weight of 120 g/m'.

The containers 'a (2) were stacked in 30 stages, and air was introduced by an air pump through an air introduction vibrator (6) provided below the support (8). The pressure of the introduced air is 0.
The pressure was 2 atm, the discharge amount was 100 cc/win, and it was closed.

The solution was introduced into the bottom of the tank through the artificial vibrator (4). The solution was domestic wastewater (mixed water of tap water, synthetic detergent, human waste, food wastewater, etc.), and the COD (according to an autoanalyzer manufactured by Technicon, USA) was 150 mg/l. Solution supply? The amount of A was 20 cc/sin, and the COD removal rate of the solution was 85% after one month of continuous treatment.

In addition, the removal rate was 85% even after three consecutive months of treatment.
The nonwoven fabric could also be easily washed.

(Example 2) A reaction for producing gluconic acid from an aqueous glucose solution was carried out using an apparatus similar to that shown in FIG. A nonwoven fabric is used as the porous body (7), and the nonwoven fabric is made of nylon 6 (3dX 5
1+us), thickness 5m++w, basis weight 150g/
+n' was used.

Add aerobic gluconic acid-producing bacteria to a 40% aqueous solution of Arakashime photocurable prepolymer (manufactured by Kansai Baint)! ! After the nonwoven fabric was immersed in the cloudy suspension, it was lightly streaked and irradiated with near ultraviolet rays for 10 minutes on each side to form gel crotches on the surface of the nonwoven fabric, thereby immobilizing bacteria on the nonwoven fabric. This nonwoven fabric was installed, and the treated water tank (1) was filled with a 10% glucose aqueous solution. Thereafter, air at a pressure of 0.2 atm was supplied from the bottom of the water tank at a flow rate of 11/gain. This produced gluconic acid, and the production rate for 5 days was 20%.

[Effects of the Invention] The immobilized physiologically active substance reaction device of the present invention can determine the water level by using a container having an overflow hole, and the water surface adjusted by the overflow hole comes into contact with the gas phase. By installing a porous material around the area, the shaking of the water surface when supplying oxygen-containing gas or solution increases the contact area between the gas phase and liquid phase, making it easier for oxygen to dissolve. The dissolved oxygen allows the reactions of physiologically active substances to occur efficiently.

The immobilized physiologically active substance reaction device of the present invention does not require forced aeration or stirring that was conventionally performed to dissolve oxygen, so it consumes less energy, helps prevent the biologically active substance from peeling off, and enables more efficient reactions. Make it.

In addition, porous materials, particularly nonwoven fabrics, have a large surface area and are suitable for fixing physiologically active substances, and also have a filtering effect, so that reactions can be carried out more effectively. In addition, since nonwoven fabrics are sheet-like and highly flexible, they can be cut into predetermined shapes, easily compressed and incinerated, and can be easily disposed of after use, giving them the advantage of being very easy to handle.

Therefore, the immobilized physiologically active substance reaction device of the present invention is extremely useful for use in bioreactors, culture vessels, water treatment devices, and the like.

[Brief explanation of the drawing]

Fig. 1 is a cross-sectional view of an example of the immobilized physiologically active substance reaction device of the present invention, Fig. 2 is an enlarged vertical cross-sectional view of a container whose vertical cross-section is 0-shaped and has an overflow hole, and Fig. 3 is a cross-sectional view of an example of the immobilized physiologically active substance reaction device of the present invention. FIG. 11 is an enlarged vertical cross-sectional view of a container with an n-shaped vertical cross section and an overflow tube; FIG. A cross-sectional view of another example of an active substance reactor; FIG. 6 is an enlarged view of a vessel having a boat-shaped longitudinal section and an overflow pipe; Explanation of symbols: Treated water tank 2: Container: Overflow hole: Artificial vibe: Exit vibe [;... Air introduction vibe: Porous body 8: Support: Overflow pipe IO... Flow hole: Convergence of the container: Height of the container: Height of the downward protrusion (Fig. 4) Drawing plan (Fig. 6)

Claims (1)

[Claims] (1) A reaction device in which a plurality of sets of a porous body (7) capable of supporting a physiologically active substance and a container (2) with one side open are arranged in multiple stages, and the container (2) is formed with an overflow hole (3) through which the solution and/or gas can move between the containers (2), and at least one open end of the overflow hole (3) is connected to the porous body (7). ) An immobilized physiologically active substance reaction device, characterized in that the device is located near the upper surface of the device.