JPH02186987A - String-shaped immobilized microorganism and production thereof - Google Patents
String-shaped immobilized microorganism and production thereofInfo
- Publication number
- JPH02186987A JPH02186987A JP487289A JP487289A JPH02186987A JP H02186987 A JPH02186987 A JP H02186987A JP 487289 A JP487289 A JP 487289A JP 487289 A JP487289 A JP 487289A JP H02186987 A JPH02186987 A JP H02186987A
- Authority
- JP
- Japan
- Prior art keywords
- string
- reinforcing body
- monomer mixture
- reinforcing material
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 48
- 238000004519 manufacturing process Methods 0.000 title claims description 16
- 239000000203 mixture Substances 0.000 claims abstract description 32
- 239000000178 monomer Substances 0.000 claims abstract description 27
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 5
- 230000003014 reinforcing effect Effects 0.000 claims description 46
- 239000000126 substance Substances 0.000 claims description 26
- 238000006116 polymerization reaction Methods 0.000 claims description 14
- 239000012779 reinforcing material Substances 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 6
- 239000012213 gelatinous substance Substances 0.000 abstract 3
- 238000009415 formwork Methods 0.000 description 12
- 238000010586 diagram Methods 0.000 description 4
- 230000002787 reinforcement Effects 0.000 description 3
- MTPJEFOSTIKRSS-UHFFFAOYSA-N 3-(dimethylamino)propanenitrile Chemical compound CN(C)CCC#N MTPJEFOSTIKRSS-UHFFFAOYSA-N 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000001879 gelation Methods 0.000 description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 2
- 238000004065 wastewater treatment Methods 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical group NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野］
本発明は、バイオリアクターに使用される固定化微生物
およびその製造方法に関する。DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to an immobilized microorganism used in a bioreactor and a method for producing the same.
一般に、廃水処理用バイオリアクターにおける汚濁物除
去媒体には、例えば、固定化微生物が使用されており、
このような固定化微生物あるいはその製造方法としては
、例えば、特開昭６２−１４４７９７号公報、特開昭６
２−１５５９９０号公報、特開昭６３−３７１５６号公
報、特願昭６０−１６８９８４号等に開示されるような
ものが知られている。Generally, immobilized microorganisms, for example, are used as pollutant removal media in bioreactors for wastewater treatment.
Examples of such immobilized microorganisms and methods for producing the same include JP-A-62-144797 and JP-A-6
Those disclosed in Japanese Patent Application Laid-open No. 2-155990, Japanese Patent Application Laid-Open No. 63-37156, Japanese Patent Application No. 168984-1984 are known.
しかしながら、このような従来の固定化微生物は、球状
あるいは板状をしていたため、量産性に劣るという問題
があった。However, such conventional immobilized microorganisms have a spherical or plate-like shape, and therefore have a problem of being inferior in mass production.
また、固定床タイプのりアクタ−における板状固定化微
生物では、充分な表面積を得ることが困難であるという
問題があった。Another problem is that it is difficult to obtain a sufficient surface area for plate-shaped immobilized microorganisms in fixed bed type glue actors.
本発明は、かかる問題を解決するためになされたもので
、従来より大幅に量産性を向上することのできる紐状固
定化微生物およびその製造方法を提供することを目的と
する。The present invention was made to solve this problem, and an object of the present invention is to provide a string-shaped immobilized microorganism and a method for producing the same, which can significantly improve mass productivity compared to the conventional method.
〔課題を解決するための手段］
本発明にかかわる紐状固定化微生物は、紐状の補強体の
外側に沿って、微生物または酵素を含むゲル状物質を固
定してなるものである。[Means for Solving the Problems] The string-like immobilized microorganism according to the present invention is formed by immobilizing a gel-like substance containing microorganisms or enzymes along the outside of a string-like reinforcing body.
また、本発明の紐状固定化微生物の製造方法は、チュー
ブ状型枠の中心部に紐状の補強体を挿通した後、前記チ
ューブ状型枠と補強体との間に、微生物または酵素を含
むモノマー混合液を注入し、このモノマー混合液を重合
反応し、得られたゲル状物質を前記補強体に固定すると
ともに、前記チューブ状型枠と補強体とを前記チューブ
状型枠の上部に新たな空間が形成されるように相対移動
し、この空間に前記モノマー混合液を注入し、この注入
されたモノマー混合液を重合反応し、得られたゲル状物
質を前記補強体に順次固定するものである。Further, in the method for producing a string-like immobilized microorganism of the present invention, after a string-like reinforcing body is inserted into the center of a tubular form, microorganisms or enzymes are inserted between the tubular form and the reinforcing body. The monomer mixture containing the monomer mixture is injected, the monomer mixture is polymerized, and the obtained gel-like substance is fixed to the reinforcing body, and the tubular form and the reinforcing body are placed on the upper part of the tubular form. The monomer mixture is relatively moved so that a new space is formed, the monomer mixture is injected into this space, the injected monomer mixture is subjected to a polymerization reaction, and the obtained gel-like substance is sequentially fixed to the reinforcing body. It is something.
本発明の紐状固定化微生物では、紐状の補強体の外側に
沿って、微生物または酵素を含むゲル状物質が固定され
る。In the string-like immobilized microorganism of the present invention, a gel-like substance containing microorganisms or enzymes is immobilized along the outside of the string-like reinforcing body.
また、本発明の紐状固定化微生物の製造方法では、チュ
ーブ状型枠の中心部に紐状の補強体が挿通された後、チ
ューブ状型枠と補強体との間に、微生物または酵素を含
むモノマー混合液が注入され、このモノマー混合液が重
合反応され、得られたゲル状物質が補強体に固定され、
この後、チューブ状型枠と補強体とがチューブ状型枠の
上部に新たな空間が形成されるように相対移動され、こ
の空間にモノマー混合液が注入され、この注入されたモ
ノマー混合液が重合反応され、得られたゲル状物質が補
強体に順次固定される。In addition, in the method for producing string-shaped immobilized microorganisms of the present invention, after the string-shaped reinforcing body is inserted into the center of the tubular form, microorganisms or enzymes are inserted between the tubular form and the reinforcing body. A monomer mixture containing the reinforcing material is injected, this monomer mixture undergoes a polymerization reaction, and the resulting gel-like substance is fixed to the reinforcing body.
After this, the tubular form and the reinforcing body are moved relative to each other so that a new space is formed at the top of the tubular form, and the monomer mixture is injected into this space. A polymerization reaction is performed, and the resulting gel-like substance is sequentially fixed to the reinforcing body.
以下、本発明の詳細を図面に示す実施例について説明す
る。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, details of the present invention will be described with reference to embodiments shown in the drawings.
第１図および第２図は、本発明の紐状固定化微生物の一
実施例を示すもので、図において符号１１は、例えば、
合成繊維あるいは天然繊維からなる紐状の補強体を示し
ている。FIG. 1 and FIG. 2 show an example of the string-shaped immobilized microorganism of the present invention, and in the figures, reference numeral 11 indicates, for example,
It shows a string-like reinforcement made of synthetic or natural fibers.
そして、この補強体１１の外側に沿って、微生物または
酵素を含むゲル状物質１３が固定されている。A gel-like substance 13 containing microorganisms or enzymes is fixed along the outside of the reinforcing body 11.
また、この実施例では、ゲル状物ｆ１３の外側には、補
強体１１に沿って、表面積を増大するため、多数の凹凸
１５が形成されている。Further, in this embodiment, a large number of irregularities 15 are formed on the outside of the gel-like material f13 along the reinforcing body 11 in order to increase the surface area.
このように構成された紐状固定化微生物は、以下述べる
ようにして製造される。The string-shaped immobilized microorganism thus configured is produced as described below.
すなわち、先ず、第３図に示すように、チューブ状型枠
１７の中心部に紐状の補強体が挿通される。That is, first, as shown in FIG. 3, a string-like reinforcing body is inserted through the center of the tubular formwork 17.
なお、この実施例では、チューブ状型枠１７の長さは、
３０ｃｍ程度とされ、その内面には、第４図に示すよう
に、軸方向に沿って多数の凹凸１９が形成されている。In addition, in this embodiment, the length of the tubular formwork 17 is as follows:
The length is approximately 30 cm, and as shown in FIG. 4, a large number of concave and convex portions 19 are formed along the axial direction on the inner surface.
また、補強体１１のゲル状物質１３の固定開始部には、
チューブ状型枠１７の下部開口を遮蔽するための蓋体２
１が固定され、この蓋体２１をチューブ状型枠エフの下
面に当接することにより遮蔽が行われている。In addition, at the fixing start part of the gel-like substance 13 of the reinforcing body 11,
Lid body 2 for shielding the lower opening of the tubular formwork 17
1 is fixed, and shielding is performed by bringing the lid 21 into contact with the lower surface of the tubular formwork F.
この後、チューブ状型枠１７と補強体１１との間に、微
生物または酵素を含むモノマー混合液が注入され、この
モノマー混合液が重合反応され、得られたゲル状物質１
３が補強体１１に固定される。Thereafter, a monomer mixture containing microorganisms or enzymes is injected between the tubular formwork 17 and the reinforcing body 11, and this monomer mixture undergoes a polymerization reaction, resulting in a gel-like substance 1.
3 is fixed to the reinforcing body 11.
すなわち、この実施例では、アクリルアミドモノマー５
〜３０重量％、架橋剤であるＢＩＳ （メチレンビスア
クリルアミド）０．１〜１重量％および微生物または酵
素を含む水溶液と、５重量％ＤＭＡＰＮ （ジメチルア
ミノプロピオニトリル）と２〜５重量％の過硫酸カリウ
ムを含む混合液がチューブ状型枠１７に注入される直前
に混合され、注液管２３を通り、チューブ状型枠１７の
上部開口から、チューブ状型枠１７と補強体１１との間
隙に充填される。That is, in this example, acrylamide monomer 5
-30% by weight, 0.1-1% by weight of BIS (methylenebisacrylamide) as a crosslinking agent, and microorganisms or enzymes, 5% by weight DMAPN (dimethylaminopropionitrile) and 2-5% by weight of filtrate. A mixed solution containing potassium sulfate is mixed immediately before being injected into the tubular form 17 , passes through the injection pipe 23 , and enters the gap between the tubular form 17 and the reinforcing body 11 from the upper opening of the tubular form 17 . is filled with.
このようにして充填された混合液は、重合反応によるゲ
ル化が進行し、５分でほぼゲル化し、ポリアクリルアミ
ドゲルからなるゲル状物質１３が補強体１１に固定され
る。The mixture filled in this manner undergoes gelation due to a polymerization reaction, becomes almost gelatinous in 5 minutes, and the gel-like substance 13 made of polyacrylamide gel is fixed to the reinforcing body 11.
この後、チューブ状型枠１７と補強体１１と力１チュー
ブ状型枠１７の上部に新たな空間が形成されるように相
対移動され、この空間にモノマー混合液が注入され、こ
の注入されたモノマー混合液が重合反応され、得られた
ゲル状物質１３が補強体１１に順次固定される。After this, the tubular formwork 17 and the reinforcement body 11 are moved relative to each other so that a new space is formed above the tubular formwork 17, and the monomer mixture is injected into this space. The monomer mixture is subjected to a polymerization reaction, and the resulting gel-like substance 13 is sequentially fixed to the reinforcing body 11.
すなわち、この実施例では、第５図に示すように、チュ
ーブ状型枠１７を固定した状態で補強体１１を下方に、
例えば、毎分６ＣＴｌｌ程度の速度で連続的に引き抜き
ながら、チューブ状型枠１７の上部開口から、チューブ
状型枠１７と補強体１１との間隙に前述した混合液を順
次供給することにより、この注入された混合液が順次重
合反応され、得られたゲル状物質１３が補強体１１に順
次固定される。That is, in this embodiment, as shown in FIG. 5, with the tubular form 17 fixed, the reinforcing body 11 is moved downward.
For example, this can be achieved by sequentially supplying the above-mentioned liquid mixture into the gap between the tubular form 17 and the reinforcing body 11 from the upper opening of the tubular form 17 while continuously drawing it out at a speed of about 6 CTll per minute. The injected liquid mixture undergoes a polymerization reaction in sequence, and the obtained gel-like substance 13 is sequentially fixed to the reinforcing body 11.
以上のように構成された紐状固定化微生物は、例えば、
第６図に示すように、得られた紐状固定化微生物２５を
、例えば、５０ｃｍ程度の長さに切断し、これ等を直方
体状の枠体２７に多数束ねることにより、バイオリアク
ターとして使用される。The string-like immobilized microorganism configured as described above is, for example,
As shown in FIG. 6, the obtained string-like immobilized microorganisms 25 are cut into lengths of, for example, about 50 cm, and a large number of them are bundled in a rectangular parallelepiped frame 27 to be used as a bioreactor. Ru.
しかして、以上のように構成された紐状固定化微生物で
は、紐状の補強体１１の外側に沿って、微生物または酵
素を含むゲル状物質１３を固定したので、従来より大幅
に量産性を向上することができる。In the string-like immobilized microorganism constructed as described above, the gel-like substance 13 containing microorganisms or enzymes is fixed along the outside of the string-like reinforcing body 11, so that mass production is much easier than in the past. can be improved.
また、以上のように構成された紐状固定化微生物では、
ゲル状物質１３の外側に凹凸１５を形成したので、ゲル
状物質１３の表面積が増大することとなるため、バイオ
リアクターとして用いた時に、処理効率を向上すること
が可能となる。In addition, in the string-shaped immobilized microorganism configured as above,
Since the unevenness 15 is formed on the outside of the gel material 13, the surface area of the gel material 13 increases, so that when used as a bioreactor, it is possible to improve the processing efficiency.
また、以上述べた紐状固定化微生物の製造方法では、チ
ューブ状型枠１７の中心部に紐状の補強体１１を挿通し
た後、チューブ状型枠１７と補強体１１との間に、微生
物または酵素を含むモノマー混合液を注入し、このモノ
マー混合液を重合反応し、得られたゲル状物質１３を補
強体１１に固定するとともに、チューブ状型枠１７と補
強体１１とをチューブ状型枠１７の上部に新たな空間２
９が形成されるように相対移動し、この空間２９にモノ
マー混合液を注入し、この注入されたモノマー混合液を
重合反応し、得られたゲル状物質１３を補強体１１に順
次固定するようにしたので、補強体１１に連続的にゲル
状物質１３を固定することが可能となり、さらに量産性
を向上することができる。In addition, in the method for producing string-shaped immobilized microorganisms described above, after the string-shaped reinforcing body 11 is inserted into the center of the tubular form 17, microorganisms are inserted between the tubular form 17 and the reinforcing body 11. Alternatively, a monomer mixture containing an enzyme is injected, this monomer mixture is subjected to a polymerization reaction, and the obtained gel-like substance 13 is fixed to the reinforcing body 11, and the tubular formwork 17 and the reinforcing body 11 are combined into a tubular mold. New space 2 above frame 17
9 is formed, a monomer mixture is injected into this space 29, the injected monomer mixture is subjected to a polymerization reaction, and the obtained gel-like substance 13 is sequentially fixed to the reinforcing body 11. Therefore, it becomes possible to continuously fix the gel-like substance 13 to the reinforcing body 11, and mass productivity can be further improved.
すなわち、例えば、第７図に示すように、チューブ状型
枠３１の中心に補強体３３を挿通し、チューブ状型枠３
１の下面開口部をゲル混合液３５内に挿入し、このチュ
ーブ状型枠３１内にゲル混合液３５を吸引し、この状態
で補強体３３にゲル状物質３７を固定することが考えら
れるが、この場合には、ゲル状物質３７とチューブ状型
枠３１との摩擦のため、ゲル状物質３７を引き抜くこと
が困難になり、５０ｃｍ以上の紐状固定化微生物を製造
することが非常に困難になるという問題が生じる。That is, for example, as shown in FIG. 7, the reinforcing body 33 is inserted into the center of the tubular form 31,
It is conceivable to insert the lower opening of 1 into the gel mixture 35, suck the gel mixture 35 into the tubular formwork 31, and fix the gel substance 37 to the reinforcing body 33 in this state. In this case, due to the friction between the gel material 37 and the tubular formwork 31, it becomes difficult to pull out the gel material 37, making it extremely difficult to produce string-shaped immobilized microorganisms with a length of 50 cm or more. The problem arises that
また、この場合には、充填すべき混合液の粘性が高いた
め、チューブ状型枠３１内に混合液を速やかに充填する
ことか困難であり、途中で重合反応が進行し、ゲル化し
てしまうという問題がある。In addition, in this case, since the mixed liquid to be filled has a high viscosity, it is difficult to quickly fill the mixed liquid into the tubular formwork 31, and a polymerization reaction progresses during the process, resulting in gelation. There is a problem.
なお、以上述べた実施例では、補強体１１を下方に移動
した例について説明したが、本発明は、かかる実施例に
限定されるものではなく、チューブ状型枠１７を上方に
移動するようにしても良いことは勿論である。In addition, in the embodiment described above, an example was explained in which the reinforcing body 11 was moved downward, but the present invention is not limited to such an embodiment, and the tubular form 17 is moved upward. Of course, it is good to do so.
また、本発明の紐状固定化微生物は、廃水処理用のバイ
オリアクターのみではなく、例えば、医薬品、化学薬品
製造等おけるバイオリアクターとしても使用できること
は勿論である。Furthermore, it goes without saying that the string-shaped immobilized microorganism of the present invention can be used not only as a bioreactor for wastewater treatment, but also as a bioreactor for, for example, manufacturing pharmaceuticals and chemicals.
以上述べたように、本発明の紐状固定化微生物では、紐
状の補強体の外側に沿って、微生物または酵素を含むゲ
ル状物質を固定したので、従来より大幅に量産性を向上
することができる。As described above, in the string-shaped immobilized microorganism of the present invention, a gel-like substance containing microorganisms or enzymes is immobilized along the outside of the string-shaped reinforcing body, so mass productivity can be significantly improved compared to the conventional method. Can be done.
また、本発明の紐状固定化微生物の製造方法では、チュ
ーブ状型枠の中心部に紐状の補強体を挿通した後、チュ
ーブ状型枠と補強体との間に、微生物または酵素を含む
モノマー混合液を注入し、このモノマー混合液を重合反
応し、得られたゲル状物質を補強体に固定するとともに
、チューブ状型枠と補強体とをチューブ状型枠の上部に
新たな空間が形成されるように相対移動し、この空間に
モノマー混合液を注入し、この注入されたモノマー混合
液を重合反応し、得られたゲル状物質を補強体に順次固
定するようにしたので、補強体に連続的にゲル状物質を
固定することが可能となり、さらに量産性を向上するこ
とができるという利点がある。In addition, in the method for producing a string-like immobilized microorganism of the present invention, after a string-like reinforcing body is inserted into the center of a tubular form, microorganisms or enzymes are inserted between the tubular form and the reinforcing body. A monomer mixture is injected, this monomer mixture undergoes a polymerization reaction, and the resulting gel-like substance is fixed to the reinforcing body, and a new space is created above the tubular form by connecting the tubular form and the reinforcing body. The monomer mixture is injected into this space, the injected monomer mixture undergoes a polymerization reaction, and the resulting gel-like substance is sequentially fixed to the reinforcing body. This method has the advantage that it becomes possible to continuously fix the gel-like substance on the body, and furthermore, mass productivity can be improved.
第１図は本発明の紐状固定化微生物の一実施例を示す縦
断面図である。
第２図は第１図の紐状固定化微生物を示す横断面図であ
る。
第３図は本発明の紐状固定化微生物の製造方法の製造開
始の状態を示す説明図である。
第４図は第３図で使用されるチューブ状型枠を示す横断
面図である。
第５図は本発明の紐状固定化微生物の製造方法の製造途
中の状態を示す説明図である。
第６図は第１図の紐状固定化微生物によりバイオリアク
ターを構成した例を示す説明口である。
第７図は本発明の紐状固定化微生物の他の例を示す説明
図である。
〔主要な部分の符号の説明〕
１１・・・補強体
１３・・・ゲル状物質
１７・・・チューブ状型枠。FIG. 1 is a longitudinal sectional view showing an embodiment of the string-shaped immobilized microorganism of the present invention. FIG. 2 is a cross-sectional view showing the string-like immobilized microorganism of FIG. 1. FIG. 3 is an explanatory diagram showing the state at the start of production of the method for producing string-shaped immobilized microorganisms of the present invention. FIG. 4 is a cross-sectional view showing the tubular form used in FIG. 3. FIG. 5 is an explanatory diagram showing a state during production of the method for producing string-shaped immobilized microorganisms of the present invention. FIG. 6 is an explanatory diagram showing an example of a bioreactor configured with the string-like immobilized microorganisms of FIG. 1. FIG. 7 is an explanatory diagram showing another example of the string-shaped immobilized microorganism of the present invention. [Explanation of symbols of main parts] 11... Reinforcement body 13... Gel-like substance 17... Tubular formwork.
Claims (2)
を含むゲル状物質を固定してなることを特徴とする紐状
固定化微生物。(1) A string-like immobilized microorganism characterized in that a gel-like substance containing microorganisms or enzymes is fixed along the outside of a string-like reinforcing body.
た後、前記チューブ状型枠と補強体との間に、微生物ま
たは酵素を含むモノマー混合液を注入し、このモノマー
混合液を重合反応し、得られたゲル状物質を前記補強体
に固定するとともに、前記チューブ状型枠と補強体とを
前記チューブ状型枠の上部に新たな空間が形成されるよ
うに相対移動し、この空間に前記モノマー混合液を注入
し、この注入されたモノマー混合液を重合反応し、得ら
れたゲル状物質を前記補強体に順次固定することを特徴
とする紐状固定化微生物の製造方法。(2) After inserting a string-like reinforcing body into the center of the tubular form, a monomer mixture containing microorganisms or enzymes is injected between the tubular form and the reinforcing body, and this monomer mixture is is subjected to a polymerization reaction, and the obtained gel-like substance is fixed to the reinforcing body, and the tubular form and the reinforcing body are moved relative to each other so that a new space is formed above the tubular form. , production of a string-shaped immobilized microorganism, characterized in that the monomer mixture is injected into this space, the injected monomer mixture is subjected to a polymerization reaction, and the obtained gel-like substance is sequentially immobilized on the reinforcing body. Method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP487289A JPH02186987A (en) | 1989-01-13 | 1989-01-13 | String-shaped immobilized microorganism and production thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP487289A JPH02186987A (en) | 1989-01-13 | 1989-01-13 | String-shaped immobilized microorganism and production thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02186987A true JPH02186987A (en) | 1990-07-23 |
| JPH0457320B2 JPH0457320B2 (en) | 1992-09-11 |
Family
ID=11595763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP487289A Granted JPH02186987A (en) | 1989-01-13 | 1989-01-13 | String-shaped immobilized microorganism and production thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02186987A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1741678A1 (en) * | 2005-06-06 | 2007-01-10 | Hitachi Plant Technologies, Ltd. | Process and apparatus for producing entrapping immobilization pellets |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3456756B2 (en) | 1994-05-30 | 2003-10-14 | 天野エンザイム株式会社 | Composition for improving quality of bread and method for producing bread using the composition |
-
1989
- 1989-01-13 JP JP487289A patent/JPH02186987A/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1741678A1 (en) * | 2005-06-06 | 2007-01-10 | Hitachi Plant Technologies, Ltd. | Process and apparatus for producing entrapping immobilization pellets |
| US8030041B2 (en) | 2005-06-06 | 2011-10-04 | Hitachi Plant Technologies, Ltd. | Process for producing pellets entrapping and immobilizing microorganisms |
| US8227238B2 (en) | 2005-06-06 | 2012-07-24 | Hitachi Plant Technologies, Ltd. | Apparatus for producing pellets containing entrapped and immobilized microorganisms |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0457320B2 (en) | 1992-09-11 |
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