JPH0218424A - Method for concentrating curdlan sol - Google Patents
Method for concentrating curdlan solInfo
- Publication number
- JPH0218424A JPH0218424A JP16748988A JP16748988A JPH0218424A JP H0218424 A JPH0218424 A JP H0218424A JP 16748988 A JP16748988 A JP 16748988A JP 16748988 A JP16748988 A JP 16748988A JP H0218424 A JPH0218424 A JP H0218424A
- Authority
- JP
- Japan
- Prior art keywords
- curdlan
- sol
- hydrated
- concentration
- pulverized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920002558 Curdlan Polymers 0.000 title claims abstract description 59
- 239000001879 Curdlan Substances 0.000 title claims abstract description 59
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 title claims abstract description 59
- 229940078035 curdlan Drugs 0.000 title claims abstract description 59
- 235000019316 curdlan Nutrition 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims description 16
- 238000010298 pulverizing process Methods 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 abstract description 16
- 238000004062 sedimentation Methods 0.000 abstract description 11
- 238000001035 drying Methods 0.000 abstract description 7
- 229920001282 polysaccharide Polymers 0.000 abstract description 7
- 239000005017 polysaccharide Substances 0.000 abstract description 7
- 239000002253 acid Substances 0.000 abstract description 6
- 150000004676 glycans Chemical class 0.000 abstract description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract description 4
- 239000008103 glucose Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000003349 gelling agent Substances 0.000 abstract description 2
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract 2
- 241000588986 Alcaligenes Species 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 238000010008 shearing Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 15
- 238000005119 centrifugation Methods 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 239000007788 liquid Substances 0.000 description 8
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 238000000926 separation method Methods 0.000 description 6
- 239000012670 alkaline solution Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 241000588813 Alcaligenes faecalis Species 0.000 description 3
- 229940005347 alcaligenes faecalis Drugs 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000001238 wet grinding Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- -1 composed of β-1 Chemical class 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000018044 dehydration Effects 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000011121 sodium hydroxide Nutrition 0.000 description 2
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 159000000003 magnesium salts Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 150000003751 zinc Chemical class 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Processes Of Treating Macromolecular Substances (AREA)
Abstract
Description
【発明の詳細な説明】
産業上の利用分野
本発明は、食品あるいは化学工業等の分野においてゲル
化剤・、増粘剤、品質改良剤等として有用なカードラン
について、その水和ゾルの濃縮法を提供するものである
。DETAILED DESCRIPTION OF THE INVENTION Field of Industrial Application The present invention relates to the concentration of hydrated sol of curdlan, which is useful as a gelling agent, thickener, quality improver, etc. in the food or chemical industries. It provides law.
従来の枝清
カードランは、β−１，３−グルコース結合を主体とす
る加熱凝固性多糖類であって、微生物によって生産され
る。従来、その製造法としては、特公昭４８−３２６７
３号あるいは特公昭４８３２６７４号に記載されている
ように、カードラン発酵液をアルカリ性にしたのち、菌
体等を分離し、次いで酸を加えてカードランを析出させ
、これを遠心分離等により濃縮し、必要に応じて乾燥す
る方法が採られている。この濃縮は、カードランと水と
の結合状態が強いために低濃度下ても容易でないことか
ら、カードランの水和ゾルに水溶性のカルシウム塩、マ
グネシウム塩または亜鉛塩を添加溶解し、次いで液性を
アルカリ性となし、次に酸を加えてカードランを析出仕
しめ、遠心分離で濃縮分離する方法が開発されている（
特公昭５２−３１０２５号）。Conventional Edasei curdlan is a heat-coagulable polysaccharide mainly composed of β-1,3-glucose bonds, and is produced by microorganisms. Conventionally, the manufacturing method was
As described in No. 3 or Japanese Patent Publication No. 4832674, after making the curdlan fermentation liquid alkaline, bacterial cells etc. are separated, then acid is added to precipitate curdlan, and this is concentrated by centrifugation etc. However, a method of drying as necessary is adopted. This concentration is not easy even at low concentrations due to the strong bond between curdlan and water. Therefore, a water-soluble calcium salt, magnesium salt, or zinc salt is added and dissolved in the hydrated sol of curdlan, and then A method has been developed in which the liquid is made alkaline, then an acid is added to precipitate curdlan, and the curdlan is concentrated and separated using centrifugation.
Special Publication No. 52-31025).
発明が解決しようとする課題
カードラン水和ゾルの濃縮法として、特公昭５２−３１
０２５号の方法は効果的ではあるが、水溶性のカルシウ
ム塩等を特別に添加する必要がある。このために、より
簡単な工程で高濃度の濃縮物を得る方法の開発が望まれ
ている。Problems to be Solved by the Invention As a method for concentrating curdlan hydrated sol,
Although the method of No. 025 is effective, it requires special addition of a water-soluble calcium salt or the like. For this reason, it is desired to develop a method for obtaining a highly concentrated concentrate through a simpler process.
そこで、本発明者らは種々の方面から検討したところ、
カードランのアルカリ溶解液を酸で析出させたゾルを、
−置屋式粉砕したあと遠心分離すると意外にも４％以上
の濃縮物が得られることを見出し、さらに検討して本発
明を完成したものである。Therefore, the present inventors investigated from various aspects and found that
A sol made by precipitating an alkaline solution of curdlan with an acid,
- It was unexpectedly discovered that a concentrate of 4% or more could be obtained by centrifuging the product after pulverization, and after further investigation, the present invention was completed.
すなわち、本発明はカードランの水和ゾルを湿式粉砕し
たのち、遠心分離することを特徴とするカードランゾル
の濃縮法である。That is, the present invention is a method for concentrating curdlan sol, which is characterized in that a hydrated sol of curdlan is wet-pulverized and then centrifuged.
本願明細書におけるカードラン水和ゾルの沈降容積比は
、次の方法を基準にして測定した。The sedimentation volume ratio of the curdlan hydrated sol in the present specification was measured based on the following method.
カードランの０．２５％（重量／容１ｋ）の水和ゾル２
０−を目′盛付共栓試験管（内径１５ｍｍ、高さ１５ｇ
ｍｍ）に取り、軽く反転させた後、２０℃で１５時間放
置後、カードラン水和ゾル液の沈降容積（−）を読みと
る。ここで沈降容積比は次式で表わされろ。Curdlan 0.25% (weight/volume 1k) hydrated sol 2
A stoppered test tube with a 0- scale (inner diameter 15 mm, height 15 g)
mm), invert it slightly, and leave it for 15 hours at 20°C, then read the sedimentation volume (-) of the curdlan hydrated sol solution. Here, the sedimentation volume ratio is expressed by the following formula.
カードランはβ−１，３−グルコース結合を主体とする
加熱凝固性多糖類であり、たとえばアルカリ土類金属ま
たはアグロバクテリウム属の閑が生産する多糖類が挙げ
られろ。具体的にはアルカリゲネス・フェカリス・バー
ル・ミクソゲネス菌株１０Ｃ３Ｋにより生産される多糖
類［アグリカルチエラル・バイオロジカル・ケミストリ
ー（Ａｇｒｉｃｕｒｉｕｒａｌ Ｂｉｏｌｏｇｉｃ
ａｌ Ｃｈｅｍｉｓｔｒｙ） ３ ０ 巻。Curdlan is a heat-coagulable polysaccharide mainly composed of β-1,3-glucose bonds, and includes polysaccharides produced by alkaline earth metals or Agrobacterium spp. Specifically, polysaccharides produced by Alcaligenes faecalis var myxogenes strain 10C3K [Agricuriural Biological Chemistry]
al Chemistry) Volume 30.
１９６頁（１９６６）］、アルカリゲネス・フェカリス
・バール・ミクソゲネス菌株１０Ｃ３にの変異株ＮＴＫ
−ｕ（Ｉ ＦＯｌ ３１４０）により生産さｈる多糖１
！ｆｊ（持分ＩＢ４ Ｂ −３２６７３）、７りｏバク
テリウム・ラジオバクター（ＩＦＯ１３１２７）および
その変異株Ｕ−１９（ＩＦｏ １３１２６）により生
産される多糖類（特公昭４８
３２６７４）などが挙げられる。196 (1966)], mutant strain NTK of Alcaligenes faecalis var myxogenes strain 10C3.
-polysaccharide 1 produced by u (I FOl 3140)
! fj (IB4 B-32673), a polysaccharide produced by Bacterium radiobacter 7 (IFO13127) and its mutant strain U-19 (IFo 13126) (Japanese Patent Publication No. 32674, 1974), and the like.
次に、本発明で対象とするカードランの水和ゾルとは、
上記のような公知方法によって微生物によって生産され
たカードランを発酵液から採取・分離する際に、該発酵
液から菌体等を分離したあとに得られる水和ゾル液が典
型例として挙げられる。この菌体等の分離までは常法に
よって実施でき、たとえばカードランを含む発酵液をｐ
Ｈ１１以上のアルカリ性にしてカードランを溶解し、次
いでイーストセパレーター等の遠心分離に付して菌体等
を除去し、カードランのアルカリ溶解液を得る。この液
に酸を加えてｐＨを９以下に調整することにより、カー
ドランが析出しゾル液が得られる。本発明方法は、カー
ドラン濃度が約０．１〜１％（重量／容量）で、ｐＨが
２〜９に調整された水和ゾルに好ましく適用される。カ
ードランの濃度は、約０．３〜０．６Ｋ（重量／容ＩＩ
）であることがより好ましく、ｐＨは６〜８でも濃縮度
を上げる効果を充分に達することができ、ｐＨ４以下の
酸性下、たとえばｐＨ２〜３にすると濃縮度をさらに上
げることができる。このときのｐＨＱ整に用いられる酸
は特に限定されないが、通常、塩酸が好ましい。一般１
こ、本発明においてカードランの水和ゾルは、沈降容積
比が約０，５〜０．７のものが対象とされる。Next, the hydrated sol of curdlan targeted in the present invention is:
When curdlan produced by microorganisms is collected and separated from a fermentation liquid by the above-mentioned known method, a typical example is a hydrated sol obtained after separating bacterial cells and the like from the fermentation liquid. This separation of bacterial cells etc. can be carried out by conventional methods, for example, the fermentation liquid containing curdlan is
The solution is made alkaline to H11 or higher to dissolve the curdlan, and then subjected to centrifugation using a yeast separator to remove bacterial cells and the like to obtain an alkaline solution of curdlan. By adding acid to this solution and adjusting the pH to 9 or less, curdlan is precipitated and a sol solution is obtained. The method of the present invention is preferably applied to a hydrated sol with a curdlan concentration of about 0.1-1% (weight/volume) and a pH adjusted to 2-9. The concentration of curdlan is approximately 0.3-0.6K (weight/volume II
) is more preferable, and the effect of increasing the concentration can be sufficiently achieved even at a pH of 6 to 8, and the concentration can be further increased under acidic conditions of pH 4 or less, for example, pH 2 to 3. The acid used for pH adjustment at this time is not particularly limited, but hydrochloric acid is usually preferred. General 1
In the present invention, the hydrated curdlan sol has a sedimentation volume ratio of about 0.5 to 0.7.
上記のようなカードランの水和ゾルは、次いで湿式粉砕
に付される。このときの粉砕は、沈降容積比が約０．８
〜０．９５となるように行なうのが好ましい。この粉砕
は、従来から公知の方法で実施できる。たとえば、粉砕
外力で分離すると、圧縮粉砕、衝撃圧縮粉砕、せん断粉
砕、摩擦粉砕のいずれのタイプも使用できる。粉砕機と
処理条件を例示すると次のとおりである。The hydrated curdlan sol as described above is then subjected to wet milling. At this time, the pulverization has a sedimentation volume ratio of approximately 0.8.
It is preferable to perform this so that the value becomes ˜0.95. This pulverization can be performed by a conventionally known method. For example, when separating by external crushing force, any type of compression crushing, impact compression crushing, shear crushing, or friction crushing can be used. Examples of the crusher and processing conditions are as follows.
湿式粉砕機 １）ＴＫホモミキサー（ＢＹ−型） ２）ＴＫマイコロイザ−（Ｍ型） ３）ＴＫホモミツクラインミル ４）マントンガラリン ５）ラニー高圧ホモジナイザー 注）ｔ）−３）は特殊機化（株）製。Wet crusher 1) TK homomixer (BY-type) 2) TK microloizer (M type) 3) TK homomitsu line mill 4) Manton Galarin 5) Ranney high pressure homogenizer Note) t)-3) is manufactured by Tokushu Kika Co., Ltd.
回転数
８０００〜１２０ＧＯｒｐｍ
４００Ｑ〜６０ＧＯｒｐｍ
６０００ｒｐｍ
処理時間
１０〜６０分
ｌパス
３パス
１〜３バス
１１０００ａｔ ｌパス
５）はシーベル機械（株）製
これらのなかでも、ホモミキサータイプのせん新粉砕機
が最も好ましく使用できる。Rotation speed 8000~120GOrpm 400Q~60GOrpm 6000rpm Processing time 10~60 minutes l Pass 3 passes 1~3 bath 11000 at l Pass 5) Manufactured by Siebel Machine Co., Ltd. Among these, the homo mixer type Senshin crusher is the most It can be used preferably.
湿式粉砕された水和ゾルは、次に遠心分離することによ
って濃縮物を得る。この遠心分離は約７．０００〜１５
，０ＯＯＧで実施できる。遠心分ｍ＊としては、バッチ
式遠心分離機（例、スキミング型、バスケット型）ある
いは分離仮型遠心分離機のいずれも用いることができる
。とりわけ、分離板型が好ましく、バッチ式型にくらべ
ると一般に約１〜１．５％はど濃縮物の旗度を高くする
ことができる。遠心分離機種とその使用条件についての
具体例を次にあげる。The wet-milled hydrated sol is then centrifuged to obtain a concentrate. This centrifugation is approximately 7,000 to 15
,0OOG. As the centrifugation m*, either a batch type centrifuge (eg, skimming type, basket type) or a temporary separation type centrifuge can be used. Particularly preferred is the separator type, which can generally increase the degree of concentration of the concentrate by about 1 to 1.5% compared to the batch type. Specific examples of centrifugal separation models and their usage conditions are listed below.
１）ＦＥｌｊＸ４１２（分離板型） ７０００Ｇ
１〜２ｍ’／ｌ１ｒ２）ＢＴｔｌＸＳ １０ （分
離板型） １５０００Ｇ １−４ｍ’／ｌ１ｒ３
）遠心沈降機（Ｓ−１０型） １７５０Ｇ バッ
チ式（注） １）および２）はアルファ（株）製、３）
は田辺鉄工新製。1) FEljX412 (separated plate type) 7000G
1-2m'/l1r2) BTtlXS 10 (separated plate type) 15000G 1-4m'/l1r3
) Centrifugal sedimentation machine (S-10 type) 1750G Batch type (Note) 1) and 2) are manufactured by Alpha Co., Ltd., 3)
is newly manufactured by Tanabe Iron Works.
かくして、従来のように、カードラン水和ゾルを単に遠
心分離を付す方法に比較して、濃縮度の高い水和ゾルが
得られる。この濃縮物は、必要に応じて、さらに親水性
有機溶媒による脱水や、乾燥処理等を施してもよい。In this way, a highly concentrated hydrated sol can be obtained compared to the conventional method of simply subjecting the curdlan hydrated sol to centrifugation. This concentrate may be further subjected to dehydration using a hydrophilic organic solvent, drying treatment, etc., if necessary.
実施例
以下に実施例を上げて、本発明をさらに具体的に説明す
る。パーセント（％）は特にことわらないかぎり、重電
／容量％を表わす。EXAMPLES The present invention will be explained in more detail with reference to Examples below. Percentage (%) represents heavy current/capacity % unless otherwise specified.
実施例１
アルカリゲネス・フェカリス・バール・ミクソゲネス菌
株ＫをＮＴＧ処理することによって誘導されたＮ Ｔ
Ｋ −ｕ株（ＩＦＯ１３１４０）を常法により培養して
、カードランを４．０％蓄積した発酵液を得に０この発
酵液に水を加えてカードラン１．６％に調整し、次いで
０．４Ｎ−ＮａＯＨ溶液になるようにカセイソーダを加
えて溶解し、遠心分離機（イーストセパレータ）を用い
て菌体を除去してカードランのアルカリ溶解液５００旋
を得た。Example 1 NT induced by treating Alcaligenes faecalis var myxogenes strain K with NTG
K-u strain (IFO13140) was cultured by a conventional method to obtain a fermentation liquid that accumulated 4.0% curdlan.Water was added to this fermentation liquid to adjust the concentration to 1.6% curdlan, and then 0.0% of curdlan was accumulated. Caustic soda was added and dissolved to make a .4N NaOH solution, and the bacterial cells were removed using a centrifuge (yeast separator) to obtain 500 turns of an alkaline solution of curdlan.
本アルカリ溶解液に水を加えて０．５％濃度の希釈液と
し、４Ｎ−ＨＣＩを用いて、タービン攪拌下にＩ）８６
．２に調整し、カードラン水和ゾル液を得た。本水和ゾ
ルの沈降容積比は０．５であった。次にこのカードラン
水和ゾル液をＴＫホモミキサー ＨＶ −Ｍ型（特殊機
化株式会社製）を用いて、８ 、 ＯＯＯｒｐｍで、１
０．３０および６０分の各時間ホモジナイズしｌこ。こ
れらの湿式粉砕された水和ゾルの沈降容積比は表１に示
すとおりである。Add water to this alkaline solution to make a diluted solution with a concentration of 0.5%, and use 4N-HCI to dilute it with I)86 under turbine stirring.
．． 2 to obtain a curdlan hydrated sol. The sedimentation volume ratio of this hydrated sol was 0.5. Next, this curdlan hydrated sol solution was mixed with a TK homomixer HV-M type (manufactured by Tokushu Kika Co., Ltd.) at 8.00 rpm and 1.
Homogenize for 0.30 and 60 minutes each. The sedimentation volume ratios of these wet-pulverized hydrated sols are shown in Table 1.
次に、これらの湿式粉砕された水和ゾル液を遠心分離機
０Ｍ−６０ｒｔＮローターＮｏ、９［トミー精工製コに
より１０分間遠心分離した［遠心効果１０２４０Ｇ］。Next, these wet-pulverized hydrated sol liquids were centrifuged for 10 minutes using a centrifugal separator 0M-60rtN rotor No. 9 [Tomy Seiko Co., Ltd.] [centrifugal effect 10240G].
得られたカードラン濃縮物の濃度を表１に示す。Table 1 shows the concentration of the obtained curdlan concentrate.
モミキサ−を用いて表２に示す条件でホモジナイズし、
湿式粉砕されたゲル液としたのち、遠心分離機ＣＭ−６
０１”ｔＮローターＮｏ、９（）ミー精工（株））を用
いて１０分間遠心分離して濃縮物を得た。カードラン濃
度の測定結果を表２に示す。Homogenize using a mixer under the conditions shown in Table 2,
After wet-pulverizing the gel solution, centrifuge CM-6
A concentrate was obtained by centrifugation for 10 minutes using a 01"tN rotor No. 9 (Mie Seiko Co., Ltd.). Table 2 shows the measurement results of curdlan concentration.
表２
一方、前述のｐＨ６，２のカードラン水和ゾル液を遠心
分離に付し、１．９２％に濃縮された液に水を加えて、
０．５％に調整した。この水和ゾルの沈降容積比は０．
５５であった。次に、ＴＫホ表１９表２の結果より、カ
ードランの水和ゾル濃縮物は、ホモジナイズの効果によ
り、約１％の濃度上昇が、また遠心効果により、約０．
５％の濃度上昇が見られた。Table 2 On the other hand, the above-mentioned pH 6.2 curdlan hydrated sol was subjected to centrifugation, and water was added to the concentrated solution to 1.92%.
It was adjusted to 0.5%. The settling volume ratio of this hydrated sol is 0.
It was 55. Next, from the results shown in Table 2 of TK Ho Table 19, the concentration of hydrated curdlan sol concentrate increases by about 1% due to the homogenization effect, and by about 0% due to the centrifugal effect.
A 5% increase in concentration was observed.
実施例２
実施例■こ示した方法で得られたカードランの水和ゾル
（０，５％）を遠心分離（前述のトミー精工製）により
約２％に濃縮し、次に水を加えて０．５％に希釈した。Example 2 Example ■ The hydrated sol of curdlan (0.5%) obtained by the method shown above was concentrated to about 2% by centrifugation (manufactured by Tomy Seiko, as mentioned above), and then water was added. Diluted to 0.5%.
本希釈液（沈降容積比０．５３）を６０分かけてＴＫホ
モジナイザーを用いて５０００ｒｐＩ１１６０分間、湿
式粉砕した（沈降容積比０６８８）。This diluted solution (sedimentation volume ratio 0.53) was wet-pulverized for 60 minutes using a TK homogenizer at 5000 rpm for 1160 minutes (sedimentation volume ratio 0688).
この水和ゾル液を分離板型遠心分離機［０Ｍ６０ ｎ
Ｎ −ｏ−ターＮｏ、９ ０ミー精工製）］を用いてＩ
Ｏ，０００ｒｐｍ（１６０００Ｇ）の条件の下に遠心
分離時間を１０分、３０分、６０分としてそれぞれに濃
縮された水和ゾル液を得た。濃縮物のカードラン島度を
測定して得られた結果を表３に示した。This hydrated sol solution was transferred to a separation plate centrifuge [0M60n
I
Concentrated hydrated sol solutions were obtained under conditions of 0,000 rpm (16,000 G) and centrifugation times of 10 minutes, 30 minutes, and 60 minutes. Table 3 shows the results obtained by measuring the curdlan degree of the concentrate.
表３
表３の結果に示されるように、カードランの水和ゾルを
湿式粉砕することにより、遠心分離後の濃縮物の濃度を
約１％以上上昇させることができる。Table 3 As shown in the results in Table 3, wet milling of the hydrated sol of curdlan can increase the concentration of the centrifuged concentrate by about 1% or more.
実施例３
実施例１と同様にして得たカードランのアルカリ溶解液
５００Ｑを水で希釈し、０．５％濃度とし、ｌ ｓ”Ｇ
Ｌ製櫂型攪拌槽で４ Ｎ−１（ＣＩを用いてｐＨ６，
５に調整しカードラン水和ゾル液を得た。Example 3 An alkaline solution of curdlan 500Q obtained in the same manner as in Example 1 was diluted with water to a concentration of 0.5%, and l s”G
4 N-1 (pH 6 using CI,
5 to obtain a curdlan hydrated sol.
本カードラン水和ゾル液を２種の分離板型遠心分離機（
長瀬アルファー（株）製ＦＥＵＸ４１２およびＢＴＵＸ
５１０）を用イテ約１．５ｍ３／Ｈｒで、遠心分離した
。This curdlan hydrated sol solution was passed through two types of separating plate centrifuges (
FEUX412 and BTUX manufactured by Nagase Alpha Co., Ltd.
510) was centrifuged at approximately 1.5 m3/Hr.
一方、同様にして得られた水和ゾル液を、ＴＫホモミキ
サー（７，５ｋｗ）を用いて約６０分湯式粉砕を行ない
、同様に分離仮型遠心分離機を用いて、約１．５ｍ’／
Ｈｒで濃縮分離し、濃縮物を得た。カードラン１度（％
）を測定して得られた結果を表４に示す。On the other hand, the hydrated sol solution obtained in the same manner was subjected to hot water pulverization for about 60 minutes using a TK homo mixer (7.5 kW), and then pulverized for about 1.5 m using a temporary separation type centrifugal separator. '／
Concentration separation was performed with Hr to obtain a concentrate. Curd run 1 degree (%
) are shown in Table 4.
表４
表４の結果から明らかなように、カードラン水和ゾルを
湿式粉砕することにより、遠心分離による濃縮の濃度を
１％以上増加させることができる。Table 4 As is clear from the results in Table 4, by wet-pulverizing the curdlan hydrated sol, the concentration obtained by centrifugation can be increased by 1% or more.
発明の効果
カードランの水和ゾル液は、カードランと水との結合が
強いために、従来のように単に分離板型遠心分離機を用
いても精々３．５〜４％の濃縮物しか得られない。本発
明のように、遠心分離を施す前にカードランの水和ゾル
を湿式粉砕することにより約監〜１．５％ら濃縮物の濃
度を上げることができる。この結果、カードランの水和
ゾルを湿式粉砕し、１５，０ＯＯＣ；の超遠心分離機を
用いることにより５〜６％あるいはそれ以上もの高濃度
な濃縮物を得ることができる。Effects of the Invention Due to the strong bond between curdlan and water, the hydrated sol of curdlan can only be concentrated by 3.5 to 4% even if a conventional separating plate centrifuge is used. I can't get it. As in the present invention, wet milling of the hydrated sol of curdlan before centrifugation can increase the concentration of the concentrate from about 1.5% to 1.5%. As a result, a highly concentrated concentrate of 5 to 6% or more can be obtained by wet-pulverizing the hydrated sol of curdlan and using a 15,000 OOC ultracentrifuge.
このように濃縮度を上げ得ることにより、後工程におい
て脱水・乾燥してカードランの乾燥物を得る際のエネル
ギー負荷を低減せしめることができる。たとえば、カー
ドラン水和ゾルの１縮度を１％上昇させることにより、
後工程における各種乾燥法において水蒸発負荷を２０〜
３０％減少させ得ることとなり、工業的に非常に有利な
ものとなる。By increasing the degree of concentration in this way, it is possible to reduce the energy load when dehydrating and drying curdlan to obtain dried curdlan in the subsequent process. For example, by increasing the degree of contraction of curdlan hydrated sol by 1%,
In various drying methods in post-processes, the water evaporation load is reduced to 20~
This can be reduced by 30%, which is very advantageous industrially.
さらに、５％以上もの高い濃度の水和ゾル液は、乾燥す
ることなく、食品あるいは化学工業の各種用途に利用し
得る機会が多くなる。すなわち、従来のような水和ゾル
の濃度では、そのまま利用すると対象物中の水分量が過
剰となり、さらに脱水・乾燥することが不可欠であった
。高濃度の濃縮物は、そのままもしくは適宜に希釈して
用いればよく、水和ゾル濃縮物として市場流通させるこ
とも可能である。Furthermore, a hydrated sol solution with a high concentration of 5% or more has many opportunities to be used for various applications in the food or chemical industries without drying. That is, if the concentration of the hydrated sol used in the past is used as is, the amount of water in the object becomes excessive, and further dehydration and drying are essential. Highly concentrated concentrates may be used as they are or diluted as appropriate, and can also be distributed on the market as hydrated sol concentrates.
Claims (1)
することを特徴とするカードランゾルの濃縮法。A method for concentrating curdlan sol, which comprises wet-pulverizing a hydrated sol of curdlan and then centrifuging it.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16748988A JPH0218424A (en) | 1988-07-05 | 1988-07-05 | Method for concentrating curdlan sol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16748988A JPH0218424A (en) | 1988-07-05 | 1988-07-05 | Method for concentrating curdlan sol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0218424A true JPH0218424A (en) | 1990-01-22 |
Family
ID=15850629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16748988A Pending JPH0218424A (en) | 1988-07-05 | 1988-07-05 | Method for concentrating curdlan sol |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0218424A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432534A (en) * | 2016-09-05 | 2017-02-22 | 通化安柏生物科技有限公司 | Liquid curdlan and production method thereof |
-
1988
- 1988-07-05 JP JP16748988A patent/JPH0218424A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106432534A (en) * | 2016-09-05 | 2017-02-22 | 通化安柏生物科技有限公司 | Liquid curdlan and production method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2541635C2 (en) | Method of producing polymer agarose from seaweed extract | |
| Lindahl | Improved soil dispersion procedures for total bacterial counts, extraction of indigenous bacteria and cell survival | |
| WO1997048730A1 (en) | Methods for processing bacterial cellulose | |
| Milburn et al. | Selective flocculation of nucleic acids, lipids, and colloidal particles from a yeast cell homogenate by polyethyleneimine, and its scale-up | |
| McGregor et al. | Factors affecting the flocculation of bacteria by chemical additives | |
| JP4061661B2 (en) | Method for treating bacterial cellulose concentrate | |
| JPH10158303A (en) | Alkali solution or gelled product of fine fibrous cellulose | |
| CA1244368A (en) | Processing of a heteropolysaccharides solution; heteropolysaccharides powder compositions and their use | |
| JPH0218424A (en) | Method for concentrating curdlan sol | |
| JPH05184378A (en) | Production of chitosan | |
| JPH09322795A (en) | Purification of water-insoluble glucan | |
| WO2023236718A1 (en) | Preparation method for polyhydroxyalkanoate aggregate | |
| JPS6158205B2 (en) | ||
| CA1308097C (en) | Polysaccharide isolation process | |
| JP2002355505A (en) | Coagulant | |
| US4921793A (en) | Bacterial process for the production of dispersants | |
| CN107417765B (en) | Method for separating and purifying recombinant protein in escherichia coli autolysis expression system | |
| JP3157528B2 (en) | Improved suspension clarification | |
| WO2019245398A1 (en) | Use of a polysaccharide microgel reagent in vegetable oil production | |
| JPH05295003A (en) | Production of beta-1,3-glucan | |
| JPH09183914A (en) | Production of tomato pigment | |
| JPH1157311A (en) | Flocculate originating from microorganism and flocculating method using same | |
| Farres et al. | A simple and efficient method for the purification of an exopolysaccharide from Klebsiella sp. I-714 | |
| JPH10226702A (en) | Method for restoring dried cellulose | |
| US3848812A (en) | Process for extracting protein from microorganisms |