JPH02163657A - Means for inspecting occult blood of feces and method for inspecting occult blood of feces - Google Patents

Abstract

PURPOSE:To limit the amt. of a specimen to the size of small-sized through-holes of an intermediate surface area by thinly applying the feces which are the specimen in the small-sized through-holes on test paper which is previously impregnated with a reagent and dried. CONSTITUTION:A rectangular sheet consisting of a hydrophobic resin, such as polyester and polyamide, is bent transversely at the points A, B and C and is thereby formed with a covering area 1, a base area 2, a protective surface area 3 and the intermediate surface area 4. The test paper 5 consisting of filter paper, cotton fabric, etc., is installed on the base area 2. The protective surface area 3 is disposed with at least >=1 pisece of the through-holes 6 on the test paper 5 and fixes the test paper 5. At least >=1 pieces of the small-sized through- holes 7 of the intermediate surface area 4 are disposed in the positions corresponding to the through-holes 6 in such a manner that the internal peripheries of the through-holes 6 protrude around the small-sized through-holes 7. The feces which are the specimen are stuck to the test paper 5 within the range of the size of the small-sized through-holes 7.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a fecal occult blood testing tool and a fecal occult blood testing method.
The present invention relates to a fecal occult blood test tool and a fecal occult blood test method for detecting blood contained in stool for early diagnosis of cancer, ulcers, etc. in the digestive tract of animals.

[Conventional technology]

BACKGROUND ART A method for chemically or immunologically detecting hemoglobin from human or animal stool specimens is known as a means for early detection of abnormalities in the digestive organs of humans or animals, such as cancer or ulcers.

In general, chemical detection methods are highly sensitive and may detect hydrogen peroxide activity, which is also found in meat and vegetables, so a false positive result may occur, so strict measures must be taken before testing. Dietary Restrictions A/Important 6 In immunological detection methods, hemoglobin is susceptible to the effects of digestive enzymes and proteases produced by intestinal bacteria, and the reaction between hemoglobin and anti-hemoglobin antibodies proceeds to a sufficiently detectable level. It takes a considerable amount of time.

As a method to improve the drawbacks of fecal occult blood, it has been carried out to use two types of occult blood reactions, a high-sensitivity method and a low-sensitivity method, in combination to determine the presence or absence of bleeding in a stool sample. A control area having a positive monitor and a negative monitor is provided at the opening of the rear plate of the sheet as shown in Japanese Patent Application Laid-open No. 57-190266 as such a fecal blood hyperemia test tool, and a control area with a positive monitor and a negative monitor is provided at the opening of the rear plate of the sheet, and a guaiac monitor is placed at a position corresponding to the opening. Feces #JIl on treated paper
It has been described that fecal occult blood is tested by smearing a dye and then applying a coloring solution from the back side of treated paper.

[Problem to be solved by the invention]

However, with such fecal occult blood test tools, the guaiac-treated paper deteriorates and the test paper becomes water-repellent, which delays the penetration of the coloring liquid into the test paper and prolongs the color reaction time. Fecal occult blood test tools that have been used for a long time have a problem in that their sensitivity decreases and they cannot be used.Furthermore, a coloring liquid is applied from the back of the test paper to check for color changes, but the test paper corresponding to the opening location - Since feces is coated on the surface, the color of the feces that is the specimen interferes with the color change of the test paper, making it difficult to determine the presence or absence of fresh blood.

[Means to solve the problem]

The inventors of the present invention have made extensive studies to improve these drawbacks, and have thus arrived at the present invention.

That is, the present invention is a flat-shaped fecal occult blood test tool formed by folding a rectangular sheet made of a hydrophobic resin into a plane area divided through folding lines provided parallel to each other in the lateral direction, A bottom paper holder on which a test paper is installed, connected to the bottom area via a folding line, and having one or more through holes in the upper part, and the through holes are arranged at the upper end of the test paper. a protective surface area; an intermediate surface area connected to the protective surface area via a folding line and having a small through hole having a smaller area than the through hole arranged at a position corresponding to the through hole by bending; This is a fecal occult blood test tool consisting of a mask area covering the acid intermediate area.

The present invention also provides a fecal occult blood testing tool in which the protective surface area has at least two through holes, and reagent paper at positions corresponding to the through holes is impregnated with different reagents.

Furthermore, the present invention is a fecal occult blood testing tool comprising an adhesive body attached to the outer or inner surface of the masking area.

Furthermore, the present invention provides a method in which a rectangular sheet made of a hydrophobic resin is folded and divided through folding lines provided parallel to each other in the lateral direction, and a flat area is impregnated with a reagent that reacts with hemoglobin. A test paper is provided, a protective area in which at least one through hole is arranged on the test paper, and an intermediate surface area in which a small through hole with a smaller area than the through hole is arranged at a position corresponding to the through hole. Using a fecal occult blood test tool, smear the fecal sample uniformly on the test paper corresponding to the small hole, then drop the coloring liquid, and check the color that appears around the inside of the hole in the protected area. This is a method to test for increased blood flow.

[Effect]

The fecal blood hyperemia testing device of the present invention thinly coats the sample stool on a test paper that has been pre-impregnated with a reagent and dried, through a small hole in the middle surface area, so that the amount of sample can be controlled through the small hole. The size of the hole can be limited.

Next, either pour the coloring liquid onto the specimen on the test paper and raise the intermediate surface area, or open and raise the intermediate surface area and then pour the coloring liquid, so that the feces are absorbed around the inside of the hole in the protective surface area. A color reaction color unrelated to the color of the stool sample appears on the unadhered test paper.

A reagent that reacts with the hemoglobin impregnated into the test strip makes up the fecal occult blood test tool.Since the rectangular sheet is made of hydrophobic resin, it does not deteriorate due to moisture and can be used after long periods of storage. .

(Example) Next, an example of the fecal occult blood testing tool of the present invention will be described based on the drawings.

FIG. 1 is a cross-sectional view of the fecal occult blood test tool, FIG. 2 is a perspective view thereof, and FIG. 3 is a plan view of a rectangular sheet.

In the figure, 1 is a masking area, 2 is a bottom area, 3 is a protective area, 4 is an intermediate area, 5 and 15 are test strips, 6 and 16 are through holes, and 7
17 is a small through hole, 8 is an adhesive body, and 9 is a folding line.

The rectangular sheet is laterally bent at points A, B and C in FIG. 1 to form a masking area 1, a bottom area 2, a protective area 3 and an intermediate area 4.

The rectangular sheet is a sheet made of a hydrophobic resin such as polyester, polyamide, polyolefin, polyvinyl chloride, polycarbonate, polyvinylidene chloride, polyacrylate, polymethacrylate, polystyrene, or synthetic paper.

A test paper 5 is placed on the bottom area 2. The test paper 5 is made of filter paper, cotton cloth, etc., and the test paper 5 is impregnated with a reagent that reacts with hemoglobin at the position corresponding to the small through hole 7 and then dried, or the test paper is obtained by impregnating and drying the reagent. It is used by making the shape slightly larger than the size of the through hole 6 and placing it at the position of the test paper corresponding to the through hole 6.

Reagents that react with hemoglobin include orthotolidine;
Examples include guaiac and tetramethylhenzine.

The protective surface area 3 is formed by bending a rectangular sheet at the bend i4B, and at least one through hole 6 is formed on the test paper 5.
, and fixes the test strip 5.

The intermediate surface area 4 is formed by being bent along the folding line C, and at least one small through hole 7 is formed in the protective surface area 3.
The inner periphery of the through hole 6 is arranged around the small through hole 7 at a position corresponding to the small through hole 7 so as not to protrude. The width around the inside is 1
A range of ˜10 mm is preferred.

=7 The through holes 6 and 7 are circular, square, etc., and preferably have the same shape. The stool sample is deposited on the test paper 5 within the size of the small through hole 7. When testing fecal occult blood using both a high-sensitivity method and a low-sensitivity method, there are two or more through-holes, and the test paper at a position corresponding to each through-hole is impregnated with a reagent.

Further, by bending the covered area l, it can be made into a flattened state. At this time, by providing an adhesive on the inner surface of the masking area 1 or the outer surface of the protection area 3, the wall surface of the protection area 3 and the wall surface of the masking area can be fixed.

Further, it is preferable that a release sheet is attached to the outer surface of the adhesive body so that the release sheet can be peeled off during use. Furthermore, when an adhesive body is provided on the outer surface of the masking area 1, both ends of the adhesive body 8 can be extended to the outer surface of the bottom area 2 to fix the entire fecal occult blood testing device flat. Examples of the adhesive body include adhesive tape, glue, adhesive coating, and the like.

To use the fecal blood hyperemia test tool of the present invention, the masking area 1 is opened and a thin layer of stool sample is applied onto a test paper 5 through the small through hole 7. Next, after dropping the colored liquid onto the specimen through the small through hole 7, the intermediate surface area 4 is opened and the presence or absence of fecal blood hyperemia is determined from the color change of the test paper 5 around the inside of the through hole 6.

As the coloring liquid, hydrogen peroxide, organic hydroperoxide such as quinone hydrobaoxide, etc. are used.

When the reagent that reacts with hemoglobin is orthotolidine, it exhibits a greenish blue color due to the hydrogen peroxide solution.

Also, in the case of guaiac, when hemoglobin is present in the feces due to the hydrogen peroxide solution, it takes on a blue color.

When there are two or more through holes, the reagent that reacts with hemoglobin impregnated into the test paper corresponding to each through hole may be different, or the amount of reagent impregnated into the test paper may be different. The presence or absence of occult blood in the feces is then determined from the test results.

Examples 1 to 5 In Figures 1 to 3, test paper 5 made of filter paper with a thickness of 0.15 mm was divided into three equal parts in the longitudinal direction. The test paper corresponding to the through hole 6 was impregnated with a 0.6% ethanol solution of orthotolidine and then dried.

The test paper corresponding to the through hole 16 was impregnated with a 10% ethanol solution of guaiac and then dried. Through holes 6 and 16 are circular with a diameter of 25 mm, and the test paper corresponding to through hole 7 contains 0.4 mg of orthotolidine and through hole 1.
The test strip corresponding to No. 6 contains 0.7 mg of guaiac. The small through holes 7 and 17 are circular with a diameter of 18 mm. In this case, the masking area 1, the bottom area 2, the protective surface area 3 and the protective area 3 are made of polyethylene synthetic paper.

After storing 3 mg of the fecal occult blood testing device thus manufactured in an aluminum/polyethylene laminate bag for 4 months, a hemoglobin aqueous solution of 0.12 to 1.2 μg of hemoglobin was applied to test paper corresponding to through holes 7 and 17, respectively.
I dripped it with a dropper so that it looked like this. Next, the durability of the test paper was tested by dropping a 3% hydrogen peroxide solution in ethanol and observing the change in color of the through holes 6 and 16. The results are shown in Table 1.

Table 1 +! ' T: orthotolidine, G: guaiac.

The indications in Table 1 were judged as follows.

◎ After dropping the coloring liquid, the color develops clearly in 1 to 2 seconds.

○ After dropping the coloring liquid, the color develops clearly within 20 seconds.

△ Slight coloration occurs 30 seconds after dropping the colored liquid.

× No change in reagent paper at all.

The presence or absence of fecal occult blood was determined from the measurement results of the T method and G method. If there is no change in the test strip in both methods, it is determined that there is no possibility of occult blood. If the T method is ○ and the G method is ×, occult blood is suspected and a retest or another test is performed.

Comparative Examples 1 to 5 In Example 1, fecal occult blood test tools using paper cardboard instead of polyethylene synthetic paper for rectangular sheets were similarly stored in aluminum/polyethylene laminate bags and durability tests were conducted. . The results are shown in Table 2.

As is clear from Table 2, a fecal occult blood testing tool using a rectangular sheet made of paper-based cardboard cannot be used for testing three months after manufacture.

Example 6 Using the fecal submersion dish test tool used in Example 1, a hemoglobin-containing fecal sample obtained by adding hemoglobin to fecal EIF4 so that the amount of hemoglobin became the value shown in Table 3 was tested in the third test.
The test paper (orthotolidine-impregnated test paper and guaiac test paper) corresponding to each of the small holes 7 and 17 in the figure was uniformly smeared.

Next, after dropping a 3% hydrogen peroxide ethanol solution onto the fecal sample, the intermediate surface area was opened up and the color change in the inner periphery of each test strip corresponding to through holes 6 and 16, where feces was not smeared, was measured as a third sample. Shown in the table. The measurement criteria are the same as in Example 1.

Comparative Example 6 In the fecal occult blood test tool used in Example 1, the small holes 7 and 17 were the same size as the holes 6 and 16, and the diameter was 2.
Hemoglobin-containing feces was smeared onto the test paper in a 5 mm circle in the same manner as in Example 6 corresponding to the through hole. Next, a 3% hydrogen peroxide ethanol solution was dropped onto the stool sample, and the color change of the stool smeared on the entire surface of each test paper corresponding to through holes 6 and 16 is shown in Table 3. measurement? S quasi is Example 6
is the same as

Table 3 As is clear from Table 3, the method of Example 6 for determining the presence or absence of occult blood in stool from the change in color around the stool is better than the method of Comparative Example 6 for determining the presence or absence of occult blood in stool. It was not affected by the color of feces and had good sensitivity.

〔effect〕

In the present invention, since the rectangular sheet constituting the fecal occult blood test tool is made of hydrophobic resin, the moisture in the mount is transferred to the test paper, unlike the conventional fecal occult blood test tool in which the mount is made of paper.
The test strip will not deteriorate. In addition, the test paper becomes t8 aqueous due to deterioration, and the permeation of the coloring liquid into the test paper becomes slow, so that the coloring reaction time does not become long. Therefore, the fecal occult blood testing device of the present invention can be stored for a long period of time and can be used at any time.

Furthermore, in the present invention, two holes of different sizes are stacked on the test paper, and the fecal sample is smeared on the size of the small hole, so the feces is not smeared around the inside of the large hole, and no coloration occurs. The color developed by the reaction appears on the test paper corresponding to the inner periphery of the large through hole, making it possible to determine the presence or absence of occult blood in the feces without being affected by the color of the feces.

[Brief explanation of the drawing]

FIG. 1 is a sectional view of the fecal occult blood test tool, FIG. 2 is a perspective view thereof, and FIG. 3 is a plan view of a rectangular sheet. In the figure, 1 is a masking area, 2 is a bottom area, 3 is a protective area, 4 is an intermediate area, 5 and 15 are test strips, 6 and 16 are through holes, and 7
17 is a small through hole, 8 is an adhesive body, and 9 is a folding line. =16 Patent applicant Nissho Co., Ltd.

Claims (4)

[Claims] (1) A flat-shaped fecal occult blood testing tool made by folding a rectangular sheet made of a hydrophobic resin into a flat area divided by folding lines provided in parallel with each other in the lateral direction, and comprising a test paper. a protective surface area that is connected to the bottom area via a folding line and has at least one through hole, and the through hole is placed on the test paper; an intermediate surface area that is connected to the protective area via a folded line, and in which a small through hole with a smaller area than the through hole is arranged at a position corresponding to the through hole by bending, and a covered area that covers the intermediate surface area; A fecal occult blood test tool consisting of. (2) The fecal occult blood test device according to claim 1, wherein the protective surface area has at least two through holes, and the reagent paper at positions corresponding to the through holes is impregnated with different reagents. (3) The fecal occult blood test tool according to claim 1 or 2, wherein an adhesive is attached to the outer or inner surface of the masking area. (4) A test paper impregnated with a reagent that reacts with hemoglobin is placed on a rectangular sheet made of a hydrophobic resin, on a flat area divided by folding through folding lines provided parallel to each other in the lateral direction. a protective area in which at least one through hole is arranged on the test paper; and an intermediate area in which a small through hole with an area smaller than that of the through hole is arranged in a position corresponding to the through hole. Using an occult blood test tool, smear the fecal sample uniformly on the test paper corresponding to the small hole, then drop the coloring liquid, and detect fecal occult blood by the coloration of the test paper that appears around the inside of the hole in the protected area. How to inspect.