JPH02157204A - Stable insecticidal preparation and stabilizer - Google Patents

Abstract

PURPOSE:To obtain the subject insecticidal preparation having improved stability of Baculovirus activity and resistant to inactivation with heat, light, etc., by compounding titanium oxide and a ligninsulfonic acid salt to an insecticidal preparation containing Baculovirus. CONSTITUTION:An insecticidal preparation containing a Baculovirus such as nuclear polyhedrosis virus is added with >=1wt.%, preferably 3-60wt.% (based on the total weight of the preparation) of a viral activity stabilizer consisting of (A) titanium oxide and (B) a ligninsulfonic acid salt at a weight ratio (A:B) of 1:(0.25-4) to prevent the inactivation of the virus with heat, light, etc., and to stabilize the insecticidal effect. The prescribed amount of the above stabilizer is preferably added in the preparation process of the above agent to exhibit excellent viral effect, however, under certain circumstances, the above preparation free from the stabilizer is diluted with water, etc., and a proper amount of the stabilizer is added to the solution.

Description

DETAILED DESCRIPTION OF THE INVENTION Field of the Invention The present invention relates to a technique for stabilizing an insecticidal formulation containing an insect nuclear polyhedrosis virus or the like as an insecticidal ingredient. More specifically, baculovirus (Bac
The present invention relates to an insecticidal formulation that stabilizes the insecticidal efficacy of U lov i rus) by preventing it from being inactivated by heat, light, etc., and to such a stabilizer.

It is well known that nuclear polyhedrosis viruses produced by insects can be used as pest control agents. It is extremely effective because it can control pests. However, in actual fields, the virus is unstable and easily inactivated by ultraviolet light, so the virus does not exhibit its original effects. However, until now, the method of stabilizing virus activity was to add various proteins, pigments, sugars, etc. immediately before spraying [Journal of Invertebrate Bathology].
f Invertebrate Pathology)
Volume 17, pages 9 to 16 (1971)), a manufacturing method that stabilizes the virus against light or heat by immobilizing the virus in a solid substrate made of inactive solid protein and/or clay (special 1987-12924), a preparation in which titanium oxide is mixed in a tree to encapsulate a virus [D. L. Bull et al.
Shahnal of Economics Entromogy (Jo
urnalof Economic Entosolo
gy) Volume 69 No. 6. Pages 731 to 736] are known.

On the other hand, lignin sulfonate is used in the field of agricultural chemicals.

Widely used as a dispersant, binder, and emulsifier.

However, its use as a stabilizer for the above-mentioned virus-containing preparations is not known.

The problem that Ming is trying to solve When spraying insect nuclear polyhedrosis virus as a material for microbial control, it is reliably effective at killing many pests due to the virus's strong infectivity and propagation power against target pests. Express. However, since it is harmless to crops and host pests, it is useful as a control agent for specific pests. However, these nuclear polyhedrosis viruses of insects are inactivated by denaturing the virus protein and inactivating the enzymes contained in the virus by heat, or by being inactivated by ultraviolet rays. effectiveness decreases.

Various stabilizing agents have been studied to solve this problem, fully utilize the inherent activity of the virus, and improve the insecticidal effect against target pests. but,
Moreover, there are no sufficient preparations, and the insecticidal effect has not been maintained for a long period of time.The object of the present invention is to provide a preparation that meets such demands.

Means for Solving the Structure of the Invention In view of the above circumstances, the present inventors conducted extensive research in order to obtain a preparation with stabilized baculovirus activity. As a result, they discovered that polyhedra containing nuclear polyhedrosis virus particles or granules containing granular disease virus particles, which are insecticidal ingredients, are liquified with titanium oxide, and the present invention was completed.

In the present invention, the baculovirus is not particularly limited, but for example, Spodoptera nuclear polyhedrosis virus, which is a nuclear polyhedrosis virus.

Examples include armyworm nuclear polyhedrosis virus, and granulosis viruses such as Kokakumonhamaki granulosis virus and Chabamaki granulosis virus. These viruses can be polyhedra or granules produced by conventional methods such as breeding host insects and propagating them, or propagating them using cultured cells. Also,
It is preferable that these be purified, or when they are released from the host larva or cultured cells used for production, they may be contaminated with impurities such as separated proteins and lipids, or the remains of the insect body. There is no particular problem with the effect.

The titanium oxide used in the present invention may be a rutile type, anatase type, or a type modified singly or in combination with metal oxides, etc. The titanium oxide that can be used is It is desirable that titanium oxide be in the form of fine particles in order to achieve a uniform formulation and to achieve an effective virus deactivation prevention effect.Also, titanium oxide is chemically and physically stable, and has acid and alkali resistance. Good luck.

Since it has excellent durability, it can also be expected to be stable in formulations.

The geninsulfonate of the present invention is generally extracted from Me+ tissues or is a geninsulfone derivative, for example,
Examples include sodium ligninsulfonate, calcium ligninsulfonate, magnesium ligninsulfonate, calcium ligninsulfonate, and the like. Furthermore, two or more of these may be used in combination.

The stabilized insecticidal formulation of the present invention contains the above-mentioned titanium oxide and lignin sulfonate in a composition ratio of 0.25 to 4 lignin sulfonate per liter of titanium oxide.
(ffiffl ratio) and when it is added in a proportion of 1% by weight or more, preferably 3% to 60% of the total weight of the powder, it exhibits a remarkable effect of preventing virus inactivation.

In order to exhibit such an excellent virus stabilizing effect, it is preferable to add a predetermined amount of such stabilizers of the present invention at the time of formulation, or in some cases, these stabilizers may be added to the formulation. It is also possible to dilute a virus preparation that does not contain a curing agent with water and add an appropriate amount to the dilution when actually using it.

Furthermore, since titanium oxide is a poorly soluble substance and is a fine powder particle, even if the amount of titanium oxide is increased, it may not be possible to obtain an effect commensurate with the amount of addition, so consider biological effects and economic efficiency. It is considered practical that the amount of titanium oxide to be blended is up to 30% by weight based on the total amount of the insecticidal preparation. However, the amount of titanium oxide and geninsulfonate to be incorporated into the formulation and their ratio are determined by the method of blending titanium oxide and geninsulfonate, the type of ligninsulfonate, the dosage form of the insecticidal formulation, It may be changed as appropriate depending on the purpose of use.

The stable insecticidal formulation of the present invention can be made into a suspension by incorporating N adjuvants and an inert phase.

It can be formulated into wettable powders, wettable powders, powders, etc.

Examples of the auxiliary agents to be blended here include surfactants, antioxidants, physical property improvers, fungicides, ultraviolet absorbers, pigments, fragrances, and the like.

Examples of surfactants that can be used in the present invention are:

Examples include polyoxyethylene alkylaryl ether, polyoxyethylene alkyl ether, polyoxyethylene sorbitan monooleate, polyethylene glycol monostearate, sodium alkylnaphthalene sulfonate, sodium alkyl sulfate salt, calcium salt of alkylbenzenesulfonate, etc. It is not particularly limited to these, and if necessary, two or more of these may be used in combination.

There are no particular limitations on the inert carrier that can be used in the present invention. For example, proteins such as casein, gelatin, sodium caseinate, and globulin, talc, kaolin, clay, waxite, bentonite, zeolite, di-
Crite, sericite, pearlite, white carbon (hydrous silicon oxide), diatom, barium carbonate, barium sulfate.

Examples include inorganic mineral powders such as zinc oxide and ilmenite.

Further, one of the stable insecticidal formulations of the present invention is a fungicide.

It can also be used in combination with preservatives, plant growth inhibitors, etc.

In the stable insecticidal preparation of the present invention, both fa titanium and genin sulfonate have the effect of preventing the virus activity of the insect virus preparation from being inactivated by heat, ultraviolet rays, etc., and maintaining the stability of the insecticidal activity. have

EXAMPLES Next, examples of formulation of stable insecticidal preparations of the present invention will be described, but the invention is not limited to these examples.

In addition, all parts in the examples indicate parts by weight.

Example 1 (Suspension agent) (1) Preparation of polyhedra of Spodoptera nuclear polyhedrosis virus. Place larvae of Spodoptera larvae in the middle stage of the 5th stage one by one in a plastic cup, and add 3 to 5% of the polyhedrosis per 1 g of artificial feed into the plastic cup.
Feed mixed with xlO' polyhedra was added and added,
Infected. This is then reared for 12 days to multiply polyhedra. The diseased and dead bodies of Spodoptera larvae infected with this virus and killed are ground and suspended in deionized water in an amount more than three times their weight. The remaining layer is removed from this suspension using a 100 mesh (0.417 mm) filter to obtain a filtrate containing polygons. The filtrate is then centrifuged to remove the supernatant, and deionized water is added and centrifuged. This centrifugation operation is repeated five times to wash the precipitated polyhedra.

Finally, add deionized water to the precipitated polyhedra to suspend them.
A polyhedral suspension of Spodoptera nuclear polyhedrosis virus is obtained. The number of polyhedrons in this liquid was measured using a microscope, and 1xlo
``Adjust to a polyhedral suspension of polyhedron/g and use it for formulation.

(2) Preparation of suspension 2.0 parts of rutile titanium oxide, 0.5 parts of polyoxyethylene nonylphenyl ether, 2.0 parts of sodium di-n-octylnaphthalene sulfonate, 3.0 fi of sodium caseinate, 16.0 parts of sodium caseinate. After adding it to an aqueous solution consisting of 5 parts and mixing and dispersing it using a homomixer, 6.0 parts of sodium ligninsulfonate was added and further dispersed. Polyhedral suspension (1xlO10 polyhedron/g) 6.0i'! 30.0 parts of this liquid was obtained by the above method. Add to J and mix evenly. Furthermore, 1.0 part of colloidal hydrated aluminum silicate, pHal adjuster (0.1M citric acid 10.2M Nax HPO4 buffer (pH = 7)
) 9.0 parts and water 54. Part θ is added to adjust the pH to 7.0, and the mixture is further mixed and dispersed to obtain a uniform suspension.

Example 2 (hydrating powder) (1) Preparation of polyhedra of armyworm nuclear polyhedrosis virus Place armyworm larvae in the middle of the 5th instar one by one in a plastic cup, and add 3x10' to 5x10 per gram of artificial feed into the plastic cup. '
feed with a mixture of polyhedrons and infect the animals. This is then reared for 12 days to multiply polyhedra. The diseased and dead bodies of armyworms infected with this virus and killed are ground by adding deionized water in an amount more than three times the body weight.
Suspend. 100 meshes (0,147
The remaining layer was removed using a filtration device (mm) to obtain a filtrate containing polyhedra, which was then washed 5 times using a centrifugal separator in the same manner as in Example 1. Finally, the precipitated polyhedra are suspended in deionized water to obtain a polyhedra suspension of armyworm nuclear polyhedrosis virus. The number of polyhedra in this liquid is measured using a microscope, and a polyhedron suspension of 1XIO'' polyhedron/g is prepared and used for formulation.

(2) Preparation of wettable powder Add 5.0 parts of calcium lignin sulfonate to 5.0 parts of rutile-type titanium oxide and mix to homogenize. To this, 3.0 parts of white carbon, 7.0 parts of diatom and the polyhedron suspension obtained above (lxlO" polyhedron/
g) Add 1.0 part of the mixture and disperse it, and mix well using a blender to form a uniform powder. Furthermore, 2.0 parts of polyoxyethylene nonylphenyl ether, 3.0 parts of sodium di-n-octylnaphthalene sulfonate, 1060 parts of glucose, and 64.0 parts of kaolin clay were added and mixed well to form a wettable powder. obtain.

Example 3 (11 grains of hydrating powder) Anatase type titanium oxide 20.0 parts, calcium lignin sulfonate 5.0 parts, white carbon 5.0
Part, polyoxyethylene nonylphenyl ether 1.
0 parts, sodium lauryl sulfate 5.0 parts, polyvinyl alcohol 2.0 parts, attapulgite clay 32
．． Add 0 parts to 100 parts of wood and mix into a homogeneous suspension using a homomixer. Into this solution, 3 ml of the same polyhedral suspension (lxlo'' polyhedron/g) as used in Example 1 was added.
Add 0.0 parts, stir and mix to form a uniform suspension, and use a spray dryer to obtain a granule wettable powder.

The stable insecticidal formulation of the present invention prevents baculovirus from being inactivated by light or heat even in actual fields. Therefore, the stability of the insecticidal effect of this virus is improved, and the insecticidal effect is sustained, which could not be obtained by spraying with conventional methods, and the insecticidal effect is significantly increased. If each acid salt is used alone, it will be deactivated within a week.

Such a virus stabilizing effect brought about by the present invention can be achieved by using titanium oxide and licunin sulfonate as stabilizers in a weight ratio of 1:0.1 to 10, more preferably 1:0.25 to 1:1. It is desirable that the ratio be 1ffi&t% or more for all the virus preparations; if this range is exceeded, the effectiveness tends to gradually decrease.

Therefore, in the present invention, the combination of titanium oxide and ritanine sulfonate is useful as a stabilizer for haculovirus, and an insecticidal formulation containing the stabilizer exhibits high insecticidal effects in the field. In addition, no symptoms of chemical damage were observed in the five crops. Therefore, the present invention is highly useful.

Next, in order to specifically demonstrate the usefulness of the stable insecticidal formulation of the present invention, test examples will be shown below.

Test Example 1 A preparation prepared according to Example 1 and Example 3 was diluted with water so that the concentration of polyhedra in the spray solution was 1 x 10 r' polyhedra/ml. After adjustment, μ equivalent to 31/10 are was sprayed on soybean planting pots.

Then I left the pot outside. Immediately after that, cut more soybean leaves from the sprayed pots and place 3 leaves in each of 5 plastic petri dishes with a diameter of 9 cm. Then, 10 Spodoptera larvae (2nd instar, 1st eye) were placed in each petri dish. Then, the petri dish was covered with filter paper (container 2) as an inner lid, and incubated at 25° C. for 2 days.

After feeding with soybean confectionery (25°C, 20 hours), one larva (one larva/larva) was placed in a plastic cup containing artificial feed.
The animals were kept individually for a further 19 days for a total of 2111 days.The number of dead animals was then investigated to determine the infection fatality rate (χ). In addition, in order to confirm the sustained effect of the drug after spraying, 1 day after spraying the spray liquid, 2
After 3 days, 3 II, 5 days, and 7] 1, soybean confections were cut out from the same sprayed pots as in Section L and the infection mortality rate (%) was determined.

This test was conducted using 50 test larvae per 1 area where the drug was sprayed. The results are shown in Table 1.

Test Example 2 Spy armyworm insecticidal effect test A preparation prepared according to Example 2 was diluted with water so that the polyhedral concentration in the spray solution was 1X10' polyhedron/ml, and the mixture was applied to beet nests planted in pots for 3 to 3 hours. An amount equivalent to ゜u/10 are was sprayed. Then I left the pot outside. Immediately thereafter, cut beet leaves from the sprayed pots and place one leaf into each of five 9 cm plastic petri dishes. Then, 10 armyworm larvae (2nd instar, 1st day) were killed in each petri dish.

Then, the petri dish was covered with filter paper (No. 2) as an inner lid, and incubated at 25°C for 2 days.

After rearing on beet leaves (25°C for 12 days), larvae were placed one by one in plastic cups containing artificial food (1
(fish/l cup) and reared individually for an additional 19 days, for a total of 21 days.

After that, the number of deaths was investigated and the infection fatality rate (%) was determined.

In addition, in order to confirm the sustained effect of the drug after spraying, 1 day, 2 days, 3 days, 5 days, and 7 days after spraying the polyhedral fluid, samples were taken from the same sprayed pots using the same method as above. , cut beet leaves, infection fatality rate (%)
I asked for

This test was conducted using 50 test larvae per 1 area where the drug was sprayed. The results are shown in Table 2.

Claims (2)

[Claims] (1) A stable insecticidal formulation comprising titanium oxide and lignin sulfonate added to an insecticidal formulation containing Baculovirus. (2) Baculovirus, which is characterized by having titanium oxide and lignin sulfonate as its main components.
A virus activity stabilizer for insecticidal preparations containing P. ovirus.